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Sequence NM_004032
GGGCATGAGACCAGCCAGGCACTGGGAAACAAGGTTTGGTGCCAGAGATTTTGGTGGCTTCCAAGACTGCTTTTTCAGAGACAGGCTCATGGACACAGCACGGATTGCAGTTGTCGGGGCAGGTGTGGTGGGGCTCTCCACGGCTGTGTGCATCTCCAAACTGGTGCCCCGATGCTCCGTTACCATCATTTCAGACAAGT
TTACTCCAGATACCACCAGTGATGTGGCAGCCGGAATGCTTATTCCTCACACTTATCCAGATACACCCATTCACACGCAGAAGCAGTGGTTCAGAGAAAC
CTTTAATCACCTCTTTGCAATTGCCAATTCTGCAGAAGCTGGAGATGCTGGTGTTCATTTGGTATCAGGGATAAAGGGAAGTGGAGGCTGGACACTCACT
CGGCGAATAGAAGACCTGTGGGAACTTCATCCGTCCTTTGACATCGTGGTCAACTGTTCAGGCCTTGGAAGCAGACAGCTTGCAGGAGACTCAAAGATTT
TCCCTGTAAGGGGCCAAGTCCTCCAAGTTCAGGCTCCCTGGGTGGAGCATTTTATCCGAGATGGCAGTGGGCTGACATATATTTATCCTGGTACATCCCA
TGTAACCCTAGGTGGAACTAGGCAAAAAGGGGACTGGAATCTGTCCCCGGATGCAGAAAATAGCAGAGAGATTCTTTCCCGATGCTGTGCTCTGGAGCCC
TCCCTCCACGGAGCCTGCAACATCAGGGAGAAGGTGGGCTTGAGGCCCTACAGGCCAGGCGTGCGACTGCAGACAGAGCTCCTTGCGCGAGATGGACAGA
GGCTGCCTGTAGTCCACCACTATGGCCATGGGAGTGGGGGCATCTCAGTGCACTGGGGCACTGCTCTGGAGGCCGCCAGGCTGGTGAGCGAGTGTGTCCA
TGCCCTCAGGACCCCCATTCCCAAGTCAAACCTGTAGATGACATAAAATGACAGCAAAGAGACTGAGAGACTGTTGATCAAAGCACAGAACAGGTTCAAA
TAACTTTTCCACTGCATGAAAGTTTAATTAGACATTTCTTTGTTTTCAACATTAGAAGTGGTGTAACATGTAAGCTGAGCACGGTGGCATGCCTATAGTC
CCAGCTACTTGAGAGGCTGAGGCAGGAGGATTGTTGAGCCCAGGAGTTTGAGTGCAATCTGGGTAACATTTTTTAGAGGTGCCAGTTCCCACCTCTAAAA
AAAACACCAACAACAAAAGAAATGATGCAACATGTAGGCTTACTTAGGAAGTCCAGCATTAATGGCATAGGGCATAAAGCTCTATTTTTTGTTAAAAATA
CTTCTTATAGAGTAAAATTTATTTAGAACTGATGTCCAAGGACCTATGCAGATTTATATGAATGTTGGAAGCTATAGAGATTTTGATATCATTTGGCTTA
TAAATCCACAGGAGGAGATAATGTATGGAAAACATTTAAATCACTGTCAATTGCAGAGCTGCCTGTGATCTTCTTAGGTTATAGCCAAGTCAGCAACATA
ATTCCTCTAAATAAAATTACATTGTGCTCACA
- refseq_DDO.F1 (forward primer)
- refseq_DDO.R1 (reverse primer)
- Single exon skipping
Frame 0
GHETSQALGNKVWCQRFWWLPRLLFQRQAHGHSTDCSCRGRCGGALHGCVHLQTGAPMLRYHHFRQV
YSRYHQ*CGSRNAYSSHLSRYTHSHAEAVVQRN
L*SPLCNCQFCRSWRCWCSFGIRDKGKWRLDTH
SANRRPVGTSSVL*HRGQLFRPWKQTACRRLKDF
PCKGPSPPSSGSLGGAFYPRWQWADIYLSWYIP
CNPRWN*AKRGLESVPGCRK*QRDSFPMLCSGA
LPPRSLQHQGEGGLEALQARRATADRAPCARWTE
AACSPPLWPWEWGHLSALGHCSGGRQAGERVCP
CPQDPHSQVKPVDDIK*QQRD*ETVDQSTEQVQ
ITFPLHESLIRHFFVFNIRSGVTCKLSTVACL*S
QLLERLRQEDC*AQEFECNLGNIF*RCQFPPLK
KTPTTKEMMQHVGLLRKSSINGIGHKALFFVKN
TSYRVKFI*N*CPRTYADLYECWKL*RF*YHLAY
KSTGGDNVWKTFKSLSIAELPVIFLGYSQVSNI
IPLNKITLCS
Frame 1
GMRPARHWETRFGARDFGGFQDCFFRDRLMDTARIAVVGAGVVGLSTAVCISKLVPRCSVTIISDK
FTPDTTSDVAAGMLIPHTYPDTPIHTQKQWFRET
FNHLFAIANSAEAGDAGVHLVSGIKGSGGWTLT
RRIEDLWELHPSFDIVVNCSGLGSRQLAGDSKI
FPVRGQVLQVQAPWVEHFIRDGSGLTYIYPGTSH
VTLGGTRQKGDWNLSPDAENSREILSRCCALEP
SLHGACNIREKVGLRPYRPGVRLQTELLARDGQ
RLPVVHHYGHGSGGISVHWGTALEAARLVSECVH
ALRTPIPKSNL*MT*NDSKETERLLIKAQNRFK
*LFHCMKV*LDISLFSTLEVV*HVS*ARWHAYS
PSYLRG*GRRIVEPRSLSAIWVTFFRGASSHL*K
KHQQQKK*CNM*AYLGSPALMA*GIKLYFLLKI
LLIE*NLFRTDVQGPMQIYMNVGSYRDFDIIWL
INPQEEIMYGKHLNHCQLQSCL*SS*VIAKSAT*
FL*IKLHCAH
Frame 2
A*DQPGTGKQGLVPEILVASKTAFSETGSWTQHGLQLSGQVWWGSPRLCASPNWCPDAPLPSFQTS
LLQIPPVMWQPECLFLTLIQIHPFTRRSSGSEK
PLITSLQLPILQKLEMLVFIWYQG*REVEAGHSL
GE*KTCGNFIRPLTSWSTVQALEADSLQETQRF
SL*GAKSSKFRLPGWSILSEMAVG*HIFILVHP
M*P*VELGKKGTGICPRMQKIAERFFPDAVLWSP
PSTEPATSGRRWA*GPTGQACDCRQSSLREMDR
GCL*STTMAMGVGASQCTGALLWRPPGW*ASVS
MPSGPPFPSQTCR*HKMTAKRLRDC*SKHRTGSN
NFSTA*KFN*TFLCFQH*KWCNM*AEHGGMPIV
PAT*EAEAGGLLSPGV*VQSG*HFLEVPVPTSK
KNTNNKRNDATCRLT*EVQH*WHRA*SSIFC*KY
FL*SKIYLELMSKDLCRFI*MLEAIEILISFGL
*IHRRR*CMENI*ITVNCRAACDLLRL*PSQQH
NSSK*NYIVLT
Sequence NM_003649
GGGCATGAGACCAGCCAGGCACTGGGAAACAAGGTTTGGTGCCAGAGATTTTGGTGGCTTCCAAGACTGCTTTTTCAGAGACAGGCTCATGGACACAGCACGGATTGCAGTTGTCGGGGCAGGTGTGGTGGGGCTCTCCACGGCTGTGTGCATCTCCAAACTGGTGCCCCGATGCTCCGTTACCATCATTTCAGACAAGT
TTACTCCAGATACCACCAGTGATGTGGCAGCCGGAATGCTTATTCCTCACACTTATCCAGATACACCCATTCACACGCAGAAGCAGTGGTTCAGAGAAAC
CTTTAATCACCTCTTTGCAATTGCCAATTCTGCAGAAGCTGGAGATGCTGGTGTTCATTTGGTATCAGGTTGGCAGATATTTCAGAGCACTCCGACTGAA
GAAGTGCCATTCTGGGCTGACGTGGTTCTGGGATTTCGAAAGATGACTGAGGCTGAGCTGAAGAAATTCCCCCAGTATGTGTTTGGTCAGGCTTTTACAA
CCCTGAAATGTGAATGCCCTGCCTACCTCCCGTGGTTGGAGAAAAGGATAAAGGGAAGTGGAGGCTGGACACTCACTCGGCGAATAGAAGACCTGTGGGA
ACTTCATCCGTCCTTTGACATCGTGGTCAACTGTTCAGGCCTTGGAAGCAGACAGCTTGCAGGAGACTCAAAGATTTTCCCTGTAAGGGGCCAAGTCCTC
CAAGTTCAGGCTCCCTGGGTGGAGCATTTTATCCGAGATGGCAGTGGGCTGACATATATTTATCCTGGTACATCCCATGTAACCCTAGGTGGAACTAGGC
AAAAAGGGGACTGGAATCTGTCCCCGGATGCAGAAAATAGCAGAGAGATTCTTTCCCGATGCTGTGCTCTGGAGCCCTCCCTCCACGGAGCCTGCAACAT
CAGGGAGAAGGTGGGCTTGAGGCCCTACAGGCCAGGCGTGCGACTGCAGACAGAGCTCCTTGCGCGAGATGGACAGAGGCTGCCTGTAGTCCACCACTAT
GGCCATGGGAGTGGGGGCATCTCAGTGCACTGGGGCACTGCTCTGGAGGCCGCCAGGCTGGTGAGCGAGTGTGTCCATGCCCTCAGGACCCCCATTCCCA
AGTCAAACCTGTAGATGACATAAAATGACAGCAAAGAGACTGAGAGACTGTTGATCAAAGCACAGAACAGGTTCAAATAACTTTTCCACTGCATGAAAGT
TTAATTAGACATTTCTTTGTTTTCAACATTAGAAGTGGTGTAACATGTAAGCTGAGCACGGTGGCATGCCTATAGTCCCAGCTACTTGAGAGGCTGAGGC
AGGAGGATTGTTGAGCCCAGGAGTTTGAGTGCAATCTGGGTAACATTTTTTAGAGGTGCCAGTTCCCACCTCTAAAAAAAACACCAACAACAAAAGAAAT
GATGCAACATGTAGGCTTACTTAGGAAGTCCAGCATTAATGGCATAGGGCATAAAGCTCTATTTTTTGTTAAAAATACTTCTTATAGAGTAAAATTTATT
TAGAACTGATGTCCAAGGACCTATGCAGATTTATATGAATGTTGGAAGCTATAGAGATTTTGATATCATTTGGCTTATAAATCCACAGGAGGAGATAATG
TATGGAAAACATTTAAATCACTGTCAATTGCAGAGCTGCCTGTGATCTTCTTAGGTTATAGCCAAGTCAGCAACATAATTCCTCTAAATAAAATTACATT
GTGCTCACA
- refseq_DDO.F1 (forward primer)
- refseq_DDO.R1 (reverse primer)
- Single exon skipping
Frame 0
GHETSQALGNKVWCQRFWWLPRLLFQRQAHGHSTDCSCRGRCGGALHGCVHLQTGAPMLRYHHFRQV
YSRYHQ*CGSRNAYSSHLSRYTHSHAEAVVQRN
L*SPLCNCQFCRSWRCWCSFGIRLADISEHSD*
RSAILG*RGSGISKDD*G*AEEIPPVCVWSGFYN
PEM*MPCLPPVVGEKDKGKWRLDTHSANRRPVG
TSSVL*HRGQLFRPWKQTACRRLKDFPCKGPSP
PSSGSLGGAFYPRWQWADIYLSWYIPCNPRWN*A
KRGLESVPGCRK*QRDSFPMLCSGALPPRSLQH
QGEGGLEALQARRATADRAPCARWTEAACSPPL
WPWEWGHLSALGHCSGGRQAGERVCPCPQDPHSQ
VKPVDDIK*QQRD*ETVDQSTEQVQITFPLHES
LIRHFFVFNIRSGVTCKLSTVACL*SQLLERLR
QEDC*AQEFECNLGNIF*RCQFPPLKKTPTTKEM
MQHVGLLRKSSINGIGHKALFFVKNTSYRVKFI
*N*CPRTYADLYECWKL*RF*YHLAYKSTGGDN
VWKTFKSLSIAELPVIFLGYSQVSNIIPLNKITL
CS
Frame 1
GMRPARHWETRFGARDFGGFQDCFFRDRLMDTARIAVVGAGVVGLSTAVCISKLVPRCSVTIISDK
FTPDTTSDVAAGMLIPHTYPDTPIHTQKQWFRET
FNHLFAIANSAEAGDAGVHLVSGWQIFQSTPTE
EVPFWADVVLGFRKMTEAELKKFPQYVFGQAFT
TLKCECPAYLPWLEKRIKGSGGWTLTRRIEDLWE
LHPSFDIVVNCSGLGSRQLAGDSKIFPVRGQVL
QVQAPWVEHFIRDGSGLTYIYPGTSHVTLGGTR
QKGDWNLSPDAENSREILSRCCALEPSLHGACNI
REKVGLRPYRPGVRLQTELLARDGQRLPVVHHY
GHGSGGISVHWGTALEAARLVSECVHALRTPIP
KSNL*MT*NDSKETERLLIKAQNRFK*LFHCMKV
*LDISLFSTLEVV*HVS*ARWHAYSPSYLRG*G
RRIVEPRSLSAIWVTFFRGASSHL*KKHQQQKK
*CNM*AYLGSPALMA*GIKLYFLLKILLIE*NLF
RTDVQGPMQIYMNVGSYRDFDIIWLINPQEEIM
YGKHLNHCQLQSCL*SS*VIAKSAT*FL*IKLH
CAH
Frame 2
A*DQPGTGKQGLVPEILVASKTAFSETGSWTQHGLQLSGQVWWGSPRLCASPNWCPDAPLPSFQTS
LLQIPPVMWQPECLFLTLIQIHPFTRRSSGSEK
PLITSLQLPILQKLEMLVFIWYQVGRYFRALRLK
KCHSGLTWFWDFER*LRLS*RNSPSMCLVRLLQ
P*NVNALPTSRGWRKG*REVEAGHSLGE*KTCG
NFIRPLTSWSTVQALEADSLQETQRFSL*GAKSS
KFRLPGWSILSEMAVG*HIFILVHPM*P*VELG
KKGTGICPRMQKIAERFFPDAVLWSPPSTEPAT
SGRRWA*GPTGQACDCRQSSLREMDRGCL*STTM
AMGVGASQCTGALLWRPPGW*ASVSMPSGPPFP
SQTCR*HKMTAKRLRDC*SKHRTGSNNFSTA*K
FN*TFLCFQH*KWCNM*AEHGGMPIVPAT*EAEA
GGLLSPGV*VQSG*HFLEVPVPTSKKNTNNKRN
DATCRLT*EVQH*WHRA*SSIFC*KYFL*SKIY
LELMSKDLCRFI*MLEAIEILISFGL*IHRRR*C
MENI*ITVNCRAACDLLRL*PSQQHNSSK*NYI
VLT
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