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Sequence NM_001921
AGGGCTTCGGGCCCCCGGGGCCAGCCGCCGTCCTGCGAACGCGCGGCGCCCGGGCCGCTGCCCCCTTCCGGCCCCGCGGCGGCCCCGCCTCCTCCCCCGACTTCCTTCCCTGAGCACGGCGGCGGCGGGGACGAGCACCGGCCTGCGCGCGGAGCCGGCACCGGATGACCCAACATGAGTGAAGTTTCCTGCAAGAAACG
GGACGACTATTTGGAATGGCCAGAGTATTTTATGGCTGTGGCCTTCTTATCAGCACAGAGAAGCAAAGATCCAAATTCCCAGGTCGGCGCCTGCATCGTG
AATTCAGAAAACAAGATTGTCGGGATTGGGTACAATGGGATGCCAAATGGGTGCAGTGATGACGTGTTGCCTTGGAGAAGGACAGCAGAGAATAAGCTGG
ACACCAAATACCCGTACGTGTGCCATGCGGAGCTGAATGCCATCATGAACAAAAATTCGACCGATGTGAAAGGCTGTAGTATGTATGTTGCCTTGTTCCC
TTGTAATGAATGCGCTAAGCTCATCATCCAGGCAGGTATAAAAGAAGTGATTTTCATGTCTGATAAATACCATGATAGTGACGAGGCAACTGCTGCGAGG
CTCCTGTTTAATATGGCCGGGGTGACATTCCGGAAATTCATACCGAAGTGCAGCAAGATTGTCATTGACTTTGATTCAATTAACAGCAGACCGAGTCAAA
AGCTTCAGTGAGTTACATCTCATTCAATCTCCAGAAGATTGGGATTATCGTCTTCTAAGAGGTTGCTAATGCCTTTCATCTTGAAGTTACACATAACTTC
TTACTAGCCAGTATGGCAAAAGTAGGCATCTAAAGAATATAAAGCCTCAAATCTTCCTTACTGTCTCTCTTGTCACATGGAATCTACATGTGTTTGAACT
ATTGCTTTAGGGTTTAAAATAGGGGAGCCTGTGGTGGCCTGGTGCACAGGGCTAGAACGAGAGTGCCTCCCCTTCTTGTGTCCTGGCTGGCTGGGATGCT
GGTGGCTCTTCAGAGGAGCATCAGCTGTCTGTCATCTGCTGCGATCCGGCAGCCTCTCTTCACTGCTACATGTGCTGGAAGGACAAATAAATAATTGTGG
TTGTGTTCTTAATGGGGACGAGCAGACACACTGATCTGAACATCTGGCCCAAGTGAAGCATGGCATATAGTGCCCTTGGAAGAAAATTAGGCCTCAAATG
ACAGTAGCATTGAAGTGTTTGCTGCAGAGTTGAGGGAAACCCCCAGCCACCCTCCCGGAATCCGAGATAGGGTGGCACATCTGTCCTGACAGACGAGGAG
TGTAACTGAACCAGGAATATTTCCTCCATTCCTGCTCTCCCACTGCACACAGGGTGGTGGCACATTATCCCTCTGGGGGGTGGGGACGCCTGTTGTTTTG
GCTCAATTTGGGTTTGTTGGTCACATGGAGCTCTTCCATTTCGTTTAGCTGAATAATGAGTTGTTCCTAGAGGAGACAGCCTGTCTCTCCTTGTTGCCCC
CAAAGCCCATGCCCTGCCGTGGTGGCAGCTGGGGCTGTGGATGGGAGGGGTCCCCAACATGGATGTGTTGCCCCTCCTCCGCATGCCAACGCAGTTCATG
TACAAGGCCCCTCTGCAACTGGAGAGAAAATTAATTCCTATCCCGTGAGTGGATTGTGAGAAATTCCACCCACGTGGAGACAGCTTACTGCAGCACTGTT
GGTGTTCGGAGCTCTTCTGTGCCCTGGCTCCATGCTTTCACCTACACAAGCATCACCTTCCTAATCACCGCGGGGCGGGGAGCGTGTGGCTGTGCCCCTT
CTCTTTAATCTCATTTAATTTTTATTAAACATGCTCAGTACCTGTGTTGAGAAAAGGCTTTCTTTATCCTAAAGATTATTACCTTTTTAAAGTGCTCTTA
TATTTTCATGAGTTTTTATTTTGTCTCTGAGATTTTGTATTCCACATTCTAGGGTATTCTGTAATTTGGCTCCTTACCAATATTATTAAAATCTTATTAA
AATCTACCCTACCAAAGCA
- refseq_DCTD.F2 (forward primer)
- refseq_DCTD.R2 (reverse primer)
- Alternative donor
Frame 0
RASGPRGQPPSCERAAPGPLPPSGPAAAPPPPPTSFPEHGGGGDEHRPARGAGTG*PNMSEVSCKKR
DDYLEWPEYFMAVAFLSAQRSKDPNSQVGACIV
NSENKIVGIGYNGMPNGCSDDVLPWRRTAENKL
DTKYPYVCHAELNAIMNKNSTDVKGCSMYVALFP
CNECAKLIIQAGIKEVIFMSDKYHDSDEATAAR
LLFNMAGVTFRKFIPKCSKIVIDFDSINSRPSQ
KLQ*VTSHSISRRLGLSSSKRLLMPFILKLHITS
Y*PVWQK*ASKEYKASNLPYCLSCHMESTCV*T
IALGFKIGEPVVAWCTGLERECLPFLCPGWLGC
WWLFRGASAVCHLLRSGSLSSLLHVLEGQINNCG
CVLNGDEQTH*SEHLAQVKHGI*CPWKKIRPQM
TVALKCLLQS*GKPPATLPESEIGWHICPDRRG
V*LNQEYFLHSCSPTAHRVVAHYPSGGWGRLLFW
LNLGLLVTWSSSISFS*IMSCS*RRQPVSPCCP
QSPCPAVVAAGAVDGRGPQHGCVAPPPHANAVH
VQGPSATGEKINSYPVSGL*EIPPTWRQLTAALL
VFGALLCPGSMLSPTQASPS*SPRGGERVAVPL
LFNLI*FLLNMLSTCVEKRLSLS*RLLPF*SAL
IFS*VFILSLRFCIPHSRVFCNLAPYQYY*NLIK
IYPTKA
Frame 1
GLRAPGASRRPANARRPGRCPLPAPRRPRLLPRLPSLSTAAAGTSTGLRAEPAPDDPT*VKFPARN
GTTIWNGQSILWLWPSYQHREAKIQIPRSAPAS*
IQKTRLSGLGTMGCQMGAVMTCCLGEGQQRISW
TPNTRTCAMRS*MPS*TKIRPM*KAVVCMLPCS
LVMNALSSSSRQV*KK*FSCLINTMIVTRQLLRG
SCLIWPG*HSGNSYRSAARLSLTLIQLTADRVK
SFSELHLIQSPEDWDYRLLRGC*CLSS*SYT*L
LTSQYGKSRHLKNIKPQIFLTVSLVTWNLHVFEL
LL*GLK*GSLWWPGAQG*NESASPSCVLAGWDA
GGSSEEHQLSVICCDPAASLHCYMCWKDK*IIV
VVFLMGTSRHTDLNIWPK*SMAYSALGRKLGLK*
Q*H*SVCCRVEGNPQPPSRNPR*GGTSVLTDEE
CN*TRNISSIPALPLHTGWWHIIPLGGGDACCF
GSIWVCWSHGALPFRLAE**VVPRGDSLSLLVAP
KAHALPWWQLGLWMGGVPNMDVLPLLRMPTQFM
YKAPLQLERKLIPIP*VDCEKFHPRGDSLLQHC
WCSELFCALAPCFHLHKHHLPNHRGAGSVWLCPF
SLISFNFY*TCSVPVLRKGFLYPKDYYLFKVLL
YFHEFLFCL*DFVFHILGYSVIWLLTNIIKILL
KSTLPK
Frame 2
GFGPPGPAAVLRTRGARAAAPFRPRGGPASSPDFLP*ARRRRGRAPACARSRHRMTQHE*SFLQET
GRLFGMARVFYGCGLLISTEKQRSKFPGRRLHR
EFRKQDCRDWVQWDAKWVQ**RVALEKDSRE*AG
HQIPVRVPCGAECHHEQKFDRCERL*YVCCLVP
L**MR*AHHPGRYKRSDFHV**IP***RGNCCE
APV*YGRGDIPEIHTEVQQDCH*L*FN*QQTESK
ASVSYISFNLQKIGIIVF*EVANAFHLEVTHNF
LLASMAKVGI*RI*SLKSSLLSLLSHGIYMCLN
YCFRV*NRGACGGLVHRARTRVPPLLVSWLAGML
VALQRSISCLSSAAIRQPLFTATCAGRTNK*LW
LCS*WGRADTLI*TSGPSEAWHIVPLEEN*ASN
DSSIEVFAAELRETPSHPPGIRDRVAHLS*QTRS
VTEPGIFPPFLLSHCTQGGGTLSLWGVGTPVVL
AQFGFVGHMELFHFV*LNNELFLEETACLSLLP
PKPMPCRGGSWGCGWEGSPTWMCCPSSACQRSSC
TRPLCNWREN*FLSREWIVRNSTHVETAYCSTV
GVRSSSVPWLHAFTYTSITFLITAGRGACGCAP
SL*SHLIFIKHAQYLC*EKAFFILKIITFLKCSY
IFMSFYFVSEILYSTF*GIL*FGSLPILLKSY*
NLPYQS
Sequence NM_001012732
AGGGCTTCGGGCCCCCGGGGCCAGCCGCCGTCCTGCGAACGCGCGGCGCCCGGGCCGCTGCCCCCTTCCGGCCCCGCGGCGGCCCCGCCTCCTCCCCCGACTTCCTTCCCTGAGCACGGCGGCGGCGGGGACGAGCACCGGCCTGCGCGCGGAGCCGGCACCGGATGGTGGGCGGGGGCCAGCCGTGCGGACCCAACATG
AGTGAAGTTTCCTGCAAGAAACGGGACGACTATTTGGAATGGCCAGAGTATTTTATGGCTGTGGCCTTCTTATCAGCACAGAGAAGCAAAGATCCAAATT
CCCAGGTCGGCGCCTGCATCGTGAATTCAGAAAACAAGATTGTCGGGATTGGGTACAATGGGATGCCAAATGGGTGCAGTGATGACGTGTTGCCTTGGAG
AAGGACAGCAGAGAATAAGCTGGACACCAAATACCCGTACGTGTGCCATGCGGAGCTGAATGCCATCATGAACAAAAATTCGACCGATGTGAAAGGCTGT
AGTATGTATGTTGCCTTGTTCCCTTGTAATGAATGCGCTAAGCTCATCATCCAGGCAGGTATAAAAGAAGTGATTTTCATGTCTGATAAATACCATGATA
GTGACGAGGCAACTGCTGCGAGGCTCCTGTTTAATATGGCCGGGGTGACATTCCGGAAATTCATACCGAAGTGCAGCAAGATTGTCATTGACTTTGATTC
AATTAACAGCAGACCGAGTCAAAAGCTTCAGTGAGTTACATCTCATTCAATCTCCAGAAGATTGGGATTATCGTCTTCTAAGAGGTTGCTAATGCCTTTC
ATCTTGAAGTTACACATAACTTCTTACTAGCCAGTATGGCAAAAGTAGGCATCTAAAGAATATAAAGCCTCAAATCTTCCTTACTGTCTCTCTTGTCACA
TGGAATCTACATGTGTTTGAACTATTGCTTTAGGGTTTAAAATAGGGGAGCCTGTGGTGGCCTGGTGCACAGGGCTAGAACGAGAGTGCCTCCCCTTCTT
GTGTCCTGGCTGGCTGGGATGCTGGTGGCTCTTCAGAGGAGCATCAGCTGTCTGTCATCTGCTGCGATCCGGCAGCCTCTCTTCACTGCTACATGTGCTG
GAAGGACAAATAAATAATTGTGGTTGTGTTCTTAATGGGGACGAGCAGACACACTGATCTGAACATCTGGCCCAAGTGAAGCATGGCATATAGTGCCCTT
GGAAGAAAATTAGGCCTCAAATGACAGTAGCATTGAAGTGTTTGCTGCAGAGTTGAGGGAAACCCCCAGCCACCCTCCCGGAATCCGAGATAGGGTGGCA
CATCTGTCCTGACAGACGAGGAGTGTAACTGAACCAGGAATATTTCCTCCATTCCTGCTCTCCCACTGCACACAGGGTGGTGGCACATTATCCCTCTGGG
GGGTGGGGACGCCTGTTGTTTTGGCTCAATTTGGGTTTGTTGGTCACATGGAGCTCTTCCATTTCGTTTAGCTGAATAATGAGTTGTTCCTAGAGGAGAC
AGCCTGTCTCTCCTTGTTGCCCCCAAAGCCCATGCCCTGCCGTGGTGGCAGCTGGGGCTGTGGATGGGAGGGGTCCCCAACATGGATGTGTTGCCCCTCC
TCCGCATGCCAACGCAGTTCATGTACAAGGCCCCTCTGCAACTGGAGAGAAAATTAATTCCTATCCCGTGAGTGGATTGTGAGAAATTCCACCCACGTGG
AGACAGCTTACTGCAGCACTGTTGGTGTTCGGAGCTCTTCTGTGCCCTGGCTCCATGCTTTCACCTACACAAGCATCACCTTCCTAATCACCGCGGGGCG
GGGAGCGTGTGGCTGTGCCCCTTCTCTTTAATCTCATTTAATTTTTATTAAACATGCTCAGTACCTGTGTTGAGAAAAGGCTTTCTTTATCCTAAAGATT
ATTACCTTTTTAAAGTGCTCTTATATTTTCATGAGTTTTTATTTTGTCTCTGAGATTTTGTATTCCACATTCTAGGGTATTCTGTAATTTGGCTCCTTAC
CAATATTATTAAAATCTTATTAAAATCTACCCTACCAAAGCA
- refseq_DCTD.F2 (forward primer)
- refseq_DCTD.R2 (reverse primer)
- Alternative donor
Frame 0
RASGPRGQPPSCERAAPGPLPPSGPAAAPPPPPTSFPEHGGGGDEHRPARGAGTGWWAGASRADPT*
VKFPARNGTTIWNGQSILWLWPSYQHREAKIQI
PRSAPAS*IQKTRLSGLGTMGCQMGAVMTCCLG
EGQQRISWTPNTRTCAMRS*MPS*TKIRPM*KAV
VCMLPCSLVMNALSSSSRQV*KK*FSCLINTMI
VTRQLLRGSCLIWPG*HSGNSYRSAARLSLTLI
QLTADRVKSFSELHLIQSPEDWDYRLLRGC*CLS
S*SYT*LLTSQYGKSRHLKNIKPQIFLTVSLVT
WNLHVFELLL*GLK*GSLWWPGAQG*NESASPS
CVLAGWDAGGSSEEHQLSVICCDPAASLHCYMCW
KDK*IIVVVFLMGTSRHTDLNIWPK*SMAYSAL
GRKLGLK*Q*H*SVCCRVEGNPQPPSRNPR*GG
TSVLTDEECN*TRNISSIPALPLHTGWWHIIPLG
GGDACCFGSIWVCWSHGALPFRLAE**VVPRGD
SLSLLVAPKAHALPWWQLGLWMGGVPNMDVLPL
LRMPTQFMYKAPLQLERKLIPIP*VDCEKFHPRG
DSLLQHCWCSELFCALAPCFHLHKHHLPNHRGA
GSVWLCPFSLISFNFY*TCSVPVLRKGFLYPKD
YYLFKVLLYFHEFLFCL*DFVFHILGYSVIWLLT
NIIKILLKSTLPK
Frame 1
GLRAPGASRRPANARRPGRCPLPAPRRPRLLPRLPSLSTAAAGTSTGLRAEPAPDGGRGPAVRTQH
E*SFLQETGRLFGMARVFYGCGLLISTEKQRSKF
PGRRLHREFRKQDCRDWVQWDAKWVQ**RVALE
KDSRE*AGHQIPVRVPCGAECHHEQKFDRCERL
*YVCCLVPL**MR*AHHPGRYKRSDFHV**IP**
*RGNCCEAPV*YGRGDIPEIHTEVQQDCH*L*F
N*QQTESKASVSYISFNLQKIGIIVF*EVANAF
HLEVTHNFLLASMAKVGI*RI*SLKSSLLSLLSH
GIYMCLNYCFRV*NRGACGGLVHRARTRVPPLL
VSWLAGMLVALQRSISCLSSAAIRQPLFTATCA
GRTNK*LWLCS*WGRADTLI*TSGPSEAWHIVPL
EEN*ASNDSSIEVFAAELRETPSHPPGIRDRVA
HLS*QTRSVTEPGIFPPFLLSHCTQGGGTLSLW
GVGTPVVLAQFGFVGHMELFHFV*LNNELFLEET
ACLSLLPPKPMPCRGGSWGCGWEGSPTWMCCPS
SACQRSSCTRPLCNWREN*FLSREWIVRNSTHV
ETAYCSTVGVRSSSVPWLHAFTYTSITFLITAGR
GACGCAPSL*SHLIFIKHAQYLC*EKAFFILKI
ITFLKCSYIFMSFYFVSEILYSTF*GIL*FGSL
PILLKSY*NLPYQS
Frame 2
GFGPPGPAAVLRTRGARAAAPFRPRGGPASSPDFLP*ARRRRGRAPACARSRHRMVGGGQPCGPNM
SEVSCKKRDDYLEWPEYFMAVAFLSAQRSKDPN
SQVGACIVNSENKIVGIGYNGMPNGCSDDVLPWR
RTAENKLDTKYPYVCHAELNAIMNKNSTDVKGC
SMYVALFPCNECAKLIIQAGIKEVIFMSDKYHD
SDEATAARLLFNMAGVTFRKFIPKCSKIVIDFDS
INSRPSQKLQ*VTSHSISRRLGLSSSKRLLMPF
ILKLHITSY*PVWQK*ASKEYKASNLPYCLSCH
MESTCV*TIALGFKIGEPVVAWCTGLERECLPFL
CPGWLGCWWLFRGASAVCHLLRSGSLSSLLHVL
EGQINNCGCVLNGDEQTH*SEHLAQVKHGI*CP
WKKIRPQMTVALKCLLQS*GKPPATLPESEIGWH
ICPDRRGV*LNQEYFLHSCSPTAHRVVAHYPSG
GWGRLLFWLNLGLLVTWSSSISFS*IMSCS*RR
QPVSPCCPQSPCPAVVAAGAVDGRGPQHGCVAPP
PHANAVHVQGPSATGEKINSYPVSGL*EIPPTW
RQLTAALLVFGALLCPGSMLSPTQASPS*SPRG
GERVAVPLLFNLI*FLLNMLSTCVEKRLSLS*RL
LPF*SALIFS*VFILSLRFCIPHSRVFCNLAPY
QYY*NLIKIYPTKA
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