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Sequence NM_001292
GGGGCACTGGTACCTCCAGGACCTGGAGTGTACTGGAAGAAATGGTGCAGTCCAGATGCATCACTGTAAGCGATACCGCTCCCCTGAACCAGACCCGTACCTGAGCTACCGATGGAAGAGGAGGAGGTCCTACAGTCGGGAACATGAAGGGAGACTGCGATACCCGTCCCGAAGGGAGCCTCCCCCACGAAGATCTCGGT
CCAGAAGCCATGACCGCCTGCCCTACCAGAGGAGGTACCGGGAGCGCCGTGACAGCGATACATACCGGTGTGAAGAGCGGAGCCCATCCTTTGGAGAGGA
CTACTATGGACCTTCACGTTCTCGTCATCGTCGGCGATCGCGGGAGAGGGGGCCATACCGGACCCGCAAGCATGCCCACCACTGCCACAAACGCCGCACC
AGGTCTTGTAGCAGCGCCTCCTCGATGAGATTGTGGGGAACCTGGGTGAAGGCACCTTTGGCAAGGTGGTGGAGTGCTTGGACCATGCCAGAGGGAAGTC
TCAGGTTGCCCTGAAGATCATCCGCAACGTGGGCAAGTACCGGGAGGCTGCCCGGCTAGAAATCAACGTGCTCAAAAAAATCAAGGAGAAGGACAAAGAA
AACAAGTTCCTGTGTGTCTTGATGTCTGACTGGTTCAACTTCCACGGTCACATGTGCATCGCCTTTGAGCTCCTGGGCAAGAACACCTTTGAGTTCCTGA
AGGAGAATAACTTCCAGCCTTACCCCCTACCACATGTCCGGCACATGGCCTACCAGCTCTGCCACGCCCTTAGATTTCTGCATGAGAATCAGCTGACCCA
TACAGACTTGAAACCAGAGAACATCCTGTTTGTGAATTCTGAGTTTGAAACCCTCTACAATGAGCACAAGAGCTGTGAGGAGAAGTCAGTGAAGAACACC
AGCATCCGAGTGGCTGACTTTGGCAGTGCCACATTTGACCATGAGCACCACACCACCATTGTGGCCACCCGTCACTATCGCCCGCCTGAGGTGATCCTTG
AGCTGGGCTGGGCACAGCCCTGTGACGTCTGGAGCATTGGCTGCATTCTCTTTGAGTACTACCGGGGCTTCACACTCTTCCAGACCCACGAAAACCGAGA
GCACCTGGTGATGATGGAGAAGATCCTAGGGCCCATCCCATCACACATGATCCACCGTACCAGGAAGCAGAAATATTTCTACAAAGGGGGCCTAGTTTGG
GATGAGAACAGCTCTGACGGCCGGTATGTGAAGGAGAACTGCAAACCTCTGAAGAGTTACATGCTCCAAGACTCCCTGGAGCACGTGCAGCTGTTTGACC
TGATGAGGAGGATGTTAGAATTTGACCCTGCCCAGCGCATCACACTGGCCGAGGCCCTGCTGCACCCCTTCTTTGCTGGCCTGACCCCTGAGGAGCGGTC
CTTCCACACCAGCCGCAACCCAAGCAGATGACAGGCACAGGCCACCGCATGAGGAGATGGAGGGCGGGACTGGGCCGCCCAGCCCCTTGACTCCAGCCTC
GACCGCCAGGCCCCAGGCCAGAGCCACCCAATGAACAGTGCAATGTGAAGGAAGGCAGGAGCCTGCAGGGGAGCAGACTTGGTGCCCAGCTGCCAGAAAG
CACAGATTTGACCCAAGCTATTTATATGTTATAAAGTTATAATAAAGTGTTTCTTACTGTTTGTA
- refseq_CLK3.F2 (forward primer)
- refseq_CLK3.R2 (reverse primer)
- Single exon skipping
Frame 0
GALVPPGPGVYWKKWCSPDASL*AIPLP*TRPVPELPMEEEEVLQSGT*RETAIPVPKGASPTKISV
QKP*PPALPEEVPGAP*QRYIPV*RAEPILWRG
LLWTFTFSSSSAIAGEGAIPDPQACPPLPQTPH
QVL*QRLLDEIVGNLGEGTFGKVVECLDHARGKS
QVALKIIRNVGKYREAARLEINVLKKIKEKDKE
NKFLCVLMSDWFNFHGHMCIAFELLGKNTFEFL
KENNFQPYPLPHVRHMAYQLCHALRFLHENQLTH
TDLKPENILFVNSEFETLYNEHKSCEEKSVKNT
SIRVADFGSATFDHEHHTTIVATRHYRPPEVIL
ELGWAQPCDVWSIGCILFEYYRGFTLFQTHENRE
HLVMMEKILGPIPSHMIHRTRKQKYFYKGGLVW
DENSSDGRYVKENCKPLKSYMLQDSLEHVQLFD
LMRRMLEFDPAQRITLAEALLHPFFAGLTPEERS
FHTSRNPSR*QAQATA*GDGGRDWAAQPLDSSL
DRQAPGQSHPMNSAM*RKAGACRGADLVPSCQK
AQI*PKLFICYKVIIKCFLLFV
Frame 1
GHWYLQDLECTGRNGAVQMHHCKRYRSPEPDPYLSYRWKRRRSYSREHEGRLRYPSRREPPPRRSR
SRSHDRLPYQRRYRERRDSDTYRCEERSPSFGED
YYGPSRSRHRRRSRERGPYRTRKHAHHCHKRRT
RSCSSASSMRLWGTWVKAPLARWWSAWTMPEGS
LRLP*RSSATWASTGRLPG*KSTCSKKSRRRTKK
TSSCVS*CLTGSTSTVTCASPLSSWARTPLSS*
RRITSSLTPYHMSGTWPTSSATPLDFCMRIS*P
IQT*NQRTSCL*ILSLKPSTMSTRAVRRSQ*RTP
ASEWLTLAVPHLTMSTTPPLWPPVTIARLR*SL
SWAGHSPVTSGALAAFSLSTTGASHSSRPTKTE
STW**WRRS*GPSHHT*STVPGSRNISTKGA*FG
MRTALTAGM*RRTANL*RVTCSKTPWSTCSCLT
**GGC*NLTLPSASHWPRPCCTPSLLA*PLRSG
PSTPAATQADDRHRPPHEEMEGGTGPPSPLTPAS
TARPQARATQ*TVQCEGRQEPAGEQTWCPAARK
HRFDPSYLYVIKL**SVSYCL
Frame 2
GTGTSRTWSVLEEMVQSRCITVSDTAPLNQTRT*ATDGRGGGPTVGNMKGDCDTRPEGSLPHEDLG
PEAMTACPTRGGTGSAVTAIHTGVKSGAHPLER
TTMDLHVLVIVGDRGRGGHTGPASMPTTATNAAP
GLVAAPPR*DCGEPG*RHLWQGGGVLGPCQREV
SGCPEDHPQRGQVPGGCPARNQRAQKNQGEGQR
KQVPVCLDV*LVQLPRSHVHRL*APGQEHL*VPE
GE*LPALPPTTCPAHGLPALPRP*ISA*ESADP
YRLETREHPVCEF*V*NPLQ*AQEL*GEVSEEH
QHPSG*LWQCHI*P*APHHHCGHPSLSPA*GDP*
AGLGTAL*RLEHWLHSL*VLPGLHTLPDPRKPR
APGDDGEDPRAHPITHDPPYQEAEIFLQRGPSL
G*EQL*RPVCEGELQTSEELHAPRLPGARAAV*P
DEEDVRI*PCPAHHTGRGPAAPLLCWPDP*GAV
LPHQPQPKQMTGTGHRMRRWRAGLGRPAP*LQP
RPPGPRPEPPNEQCNVKEGRSLQGSRLGAQLPES
TDLTQAIYML*SYNKVFLTVC
Sequence NM_003992
GGGGCACTGGTACCTCCAGGACCTGGAGTGTACTGGAAGAAATGGTGCAGTCCAGATGCATCACTGTAAGCGATACCGCTCCCCTGAACCAGACCCGTACCTGAGCTACCGATGGAAGAGGAGGAGGTCCTACAGTCGGGAACATGAAGGGAGACTGCGATACCCGTCCCGAAGGGAGCCTCCCCCACGAAGATCTCGGT
CCAGAAGCCATGACCGCCTGCCCTACCAGAGGAGGTACCGGGAGCGCCGTGACAGCGATACATACCGGTGTGAAGAGCGGAGCCCATCCTTTGGAGAGGA
CTACTATGGACCTTCACGTTCTCGTCATCGTCGGCGATCGCGGGAGAGGGGGCCATACCGGACCCGCAAGCATGCCCACCACTGCCACAAACGCCGCACC
AGGTCTTGTAGCAGCGCCTCCTCGAGAAGCCAACAGAGCAGTAAGCGCAGCAGCCGGAGTGTGGAAGATGACAAGGAGGGTCACCTGGTGTGCCGGATCG
GCGATTGGCTCCAAGAGCGATATGAGATTGTGGGGAACCTGGGTGAAGGCACCTTTGGCAAGGTGGTGGAGTGCTTGGACCATGCCAGAGGGAAGTCTCA
GGTTGCCCTGAAGATCATCCGCAACGTGGGCAAGTACCGGGAGGCTGCCCGGCTAGAAATCAACGTGCTCAAAAAAATCAAGGAGAAGGACAAAGAAAAC
AAGTTCCTGTGTGTCTTGATGTCTGACTGGTTCAACTTCCACGGTCACATGTGCATCGCCTTTGAGCTCCTGGGCAAGAACACCTTTGAGTTCCTGAAGG
AGAATAACTTCCAGCCTTACCCCCTACCACATGTCCGGCACATGGCCTACCAGCTCTGCCACGCCCTTAGATTTCTGCATGAGAATCAGCTGACCCATAC
AGACTTGAAACCAGAGAACATCCTGTTTGTGAATTCTGAGTTTGAAACCCTCTACAATGAGCACAAGAGCTGTGAGGAGAAGTCAGTGAAGAACACCAGC
ATCCGAGTGGCTGACTTTGGCAGTGCCACATTTGACCATGAGCACCACACCACCATTGTGGCCACCCGTCACTATCGCCCGCCTGAGGTGATCCTTGAGC
TGGGCTGGGCACAGCCCTGTGACGTCTGGAGCATTGGCTGCATTCTCTTTGAGTACTACCGGGGCTTCACACTCTTCCAGACCCACGAAAACCGAGAGCA
CCTGGTGATGATGGAGAAGATCCTAGGGCCCATCCCATCACACATGATCCACCGTACCAGGAAGCAGAAATATTTCTACAAAGGGGGCCTAGTTTGGGAT
GAGAACAGCTCTGACGGCCGGTATGTGAAGGAGAACTGCAAACCTCTGAAGAGTTACATGCTCCAAGACTCCCTGGAGCACGTGCAGCTGTTTGACCTGA
TGAGGAGGATGTTAGAATTTGACCCTGCCCAGCGCATCACACTGGCCGAGGCCCTGCTGCACCCCTTCTTTGCTGGCCTGACCCCTGAGGAGCGGTCCTT
CCACACCAGCCGCAACCCAAGCAGATGACAGGCACAGGCCACCGCATGAGGAGATGGAGGGCGGGACTGGGCCGCCCAGCCCCTTGACTCCAGCCTCGAC
CGCCAGGCCCCAGGCCAGAGCCACCCAATGAACAGTGCAATGTGAAGGAAGGCAGGAGCCTGCAGGGGAGCAGACTTGGTGCCCAGCTGCCAGAAAGCAC
AGATTTGACCCAAGCTATTTATATGTTATAAAGTTATAATAAAGTGTTTCTTACTGTTTGTA
- refseq_CLK3.F2 (forward primer)
- refseq_CLK3.R2 (reverse primer)
- Single exon skipping
Frame 0
GALVPPGPGVYWKKWCSPDASL*AIPLP*TRPVPELPMEEEEVLQSGT*RETAIPVPKGASPTKISV
QKP*PPALPEEVPGAP*QRYIPV*RAEPILWRG
LLWTFTFSSSSAIAGEGAIPDPQACPPLPQTPH
QVL*QRLLEKPTEQ*AQQPECGR*QGGSPGVPDR
RLAPRAI*DCGEPG*RHLWQGGGVLGPCQREVS
GCPEDHPQRGQVPGGCPARNQRAQKNQGEGQRK
QVPVCLDV*LVQLPRSHVHRL*APGQEHL*VPEG
E*LPALPPTTCPAHGLPALPRP*ISA*ESADPY
RLETREHPVCEF*V*NPLQ*AQEL*GEVSEEHQ
HPSG*LWQCHI*P*APHHHCGHPSLSPA*GDP*A
GLGTAL*RLEHWLHSL*VLPGLHTLPDPRKPRA
PGDDGEDPRAHPITHDPPYQEAEIFLQRGPSLG
*EQL*RPVCEGELQTSEELHAPRLPGARAAV*PD
EEDVRI*PCPAHHTGRGPAAPLLCWPDP*GAVL
PHQPQPKQMTGTGHRMRRWRAGLGRPAP*LQPR
PPGPRPEPPNEQCNVKEGRSLQGSRLGAQLPEST
DLTQAIYML*SYNKVFLTVC
Frame 1
GHWYLQDLECTGRNGAVQMHHCKRYRSPEPDPYLSYRWKRRRSYSREHEGRLRYPSRREPPPRRSR
SRSHDRLPYQRRYRERRDSDTYRCEERSPSFGED
YYGPSRSRHRRRSRERGPYRTRKHAHHCHKRRT
RSCSSASSRSQQSSKRSSRSVEDDKEGHLVCRI
GDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQ
VALKIIRNVGKYREAARLEINVLKKIKEKDKEN
KFLCVLMSDWFNFHGHMCIAFELLGKNTFEFLK
ENNFQPYPLPHVRHMAYQLCHALRFLHENQLTHT
DLKPENILFVNSEFETLYNEHKSCEEKSVKNTS
IRVADFGSATFDHEHHTTIVATRHYRPPEVILE
LGWAQPCDVWSIGCILFEYYRGFTLFQTHENREH
LVMMEKILGPIPSHMIHRTRKQKYFYKGGLVWD
ENSSDGRYVKENCKPLKSYMLQDSLEHVQLFDL
MRRMLEFDPAQRITLAEALLHPFFAGLTPEERSF
HTSRNPSR*QAQATA*GDGGRDWAAQPLDSSLD
RQAPGQSHPMNSAM*RKAGACRGADLVPSCQKA
QI*PKLFICYKVIIKCFLLFV
Frame 2
GTGTSRTWSVLEEMVQSRCITVSDTAPLNQTRT*ATDGRGGGPTVGNMKGDCDTRPEGSLPHEDLG
PEAMTACPTRGGTGSAVTAIHTGVKSGAHPLER
TTMDLHVLVIVGDRGRGGHTGPASMPTTATNAAP
GLVAAPPREANRAVSAAAGVWKMTRRVTWCAGS
AIGSKSDMRLWGTWVKAPLARWWSAWTMPEGSL
RLP*RSSATWASTGRLPG*KSTCSKKSRRRTKKT
SSCVS*CLTGSTSTVTCASPLSSWARTPLSS*R
RITSSLTPYHMSGTWPTSSATPLDFCMRIS*PI
QT*NQRTSCL*ILSLKPSTMSTRAVRRSQ*RTPA
SEWLTLAVPHLTMSTTPPLWPPVTIARLR*SLS
WAGHSPVTSGALAAFSLSTTGASHSSRPTKTES
TW**WRRS*GPSHHT*STVPGSRNISTKGA*FGM
RTALTAGM*RRTANL*RVTCSKTPWSTCSCLT*
*GGC*NLTLPSASHWPRPCCTPSLLA*PLRSGP
STPAATQADDRHRPPHEEMEGGTGPPSPLTPAST
ARPQARATQ*TVQCEGRQEPAGEQTWCPAARKH
RFDPSYLYVIKL**SVSYCL
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