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Sequences

Sequence NM_020674

GGACAGAGCACTACTAACTCCTAACTTTTCTCAGAAAGCTGGGCTCTGCTTTCGCCAGCAACCCGGTCCGAAGTCGCGGGCATATTCTGTCTGAAATCGT GTGCACCGAAATCCCCGCCTTGCGGTGGAGGCTGGCGCTAGGCGGCCTCAGCCTCGGCCTGCTGCGCTCAGGAACCCGCGCCCCGGCTCCTCGGCGATCC ATTGCTCTTTCCTCTGGCGCCGGCCGCAGGCCTCGGTCACGCCCCCAGCGGCCCGTTGGTTTCCGGGTCCCGCGGGGTGCCCCCGCCCACACGCTATGCC TTAAATTGGGCCAGGCTGAGGCGCTGCTGCTGGAGCGGCCGATCCGAGACGTGGCTCCCTGGGCGGCAGAACCATGTTGGACTTCGCGATCTTCGCCGTT ACCTTCTTGCTGGCGTTGGTGGGAGCCGTGCTCTACCTCTATCCGGCTTCCAGACAAGCTGCAGGAATTCCAGGGATTACTCCAACTGAAGAAAAAGATG GTAATCTTCCAGATATTGTGAATAGTGGAAGTTTGCATGAGTTCCTGGTTAATTTGCATGAGAGATATGGGCCTGTGGTCTCCTTCTGGTTTGGCAGGCG CCTCGTGGTTAGTTTGGGCACTGTTGATGTACTGAAGCAGCATATCAATCCCAATAAGACATCGGACCCTTTTGAAACCATGCTGAAGTCATTATTAAGG TATCAATCTGGTGGTGGCAGTGTGAGTGAAAACCACATGAGGAAAAAATTGTATGAAAATGGTGTGACTGATTCTCTGAAGAGTAACTTTGCCCTCCTCC TAAAGCTTTCAGAAGAATTATTAGATAAATGGCTCTCCTACCCAGAGACCCAGCACGTGCCCCTCAGCCAGCATATGCTTGGTTTTGCTATGAAGTCTGT TACACAGATGGTAATGGGTAGTACATTTGAAGATGATCAGGAAGTCATTCGCTTCCAGAAGAATCATGGCACACCCTCATGCAACTGGAGTCTGTTTTAA GGAACATCATAAAAGAACGAAAAGGAAGGAACTTCAGTCAACATATTTTCATTGACTCCTTAGTACAAGGGAACCTTAATGACCAACAGATCCTAGAAGA CAGTATGATATTTTCTCTGGCCAGTTGCATAATAACTGCAAAATTGTGTACCTGGGCAATCTGTTTTTTAACCACCTCTGAAGAAGTTCAAAAAAAATTA TATGAAGAGATAAACCAAGTTTTTGGAAATGGTCCTGTTACTCCAGAGAAAATTGAGCAGCTCAGATATTGTCAGCATGTGCTTTGTGAAACTGTTCGAA CTGCCAAACTGACTCCAGTTTCTGCCCAGCTTCAAGATATTGAAGGAAAAATTGACCGATTTATTATTCCTAGAGAGACCCTCGTCCTTTATGCCCTTGG TGTGGTACTTCAGGATCCTAATACTTGGCCATCTCCACACAAGTTTGATCCAGATCGGTTTGATGATGAATTAGTAATGAAAACTTTTTCCTCACTTGGA TTCTCAGGCACACAGGAGTGTCCAGAGTTGAGGTTTGCATATATGGTGACCACAGTACTTCTTAGTGTATTGGTGAAGAGACTGCACCTACTTTCTGTGG AGGGACAGGTTATTGAAACAAAGTATGAACTGGTAACATCATCAAGGGAAGAAGCTTGGATCACTGTCTCAAAGAGATATTAAAATTTTATACATTTAAA ATCATTGTTAAATTGATTGAGGAAAACAACCATTTAAAAAAAATCTATGTTGAATCCTTTTATAAACCAGTATCACTTTGTAATATAAACACCTATTTGT ACTTAATTTTGTAAATTTGGATTTTTATATATCATATTTTCTTAATTCATTGTACACATTTGACTTACTGCACAGTATATTGATCATTTTAATGGGAAAC TTTAGCTTTCTACTTTTTATTTTTGTTTTTTCACTTTCTATGCCATTATTTTTGTATTCTTTTTCTTAGTGTGAGCTCTAAAATCAATGTTCTTGAAAAA GAAATTATTTTG

refseq_CYP20A1.F1 (forward primer)
refseq_CYP20A1.R1 (reverse primer)
Single exon skipping

Frame 0

GQSTTNS*LFSESWALLSPATRSEVAGIFCLKS CAPKSPPCGGGWR*AASASACCAQEPAPRLLGDP LLFPLAPAAGLGHAPSGPLVSGSRGVPPPTRYA LNWARLRRCCWSGRSETWLPGRQNHVGLRDLRR YLLAGVGGSRALPLSGFQTSCRNSRDYSN*RKRW *SSRYCE*WKFA*VPG*FA*EIWACGLLLVWQA PRG*FGHC*CTEAAYQSQ*DIGPF*NHAEVIIK VSIWWWQCE*KPHEEKIV*KWCD*FSEE*LCPPP KAFRRIIR*MALLPRDPARAPQPAYAWFCYEVC YTDGNG*YI*R*SGSHSLPEESWHTLMQLESVL RNIIKERKGRNFSQHIFIDSLVQGNLNDQQILED SMIFSLASCIITAKLCTWAICFLTTSEEVQKKL YEEINQVFGNGPVTPEKIEQLRYCQHVLCETVR TAKLTPVSAQLQDIEGKIDRFIIPRETLVLYALG VVLQDPNTWPSPHKFDPDRFDDELVMKTFSSLG FSGTQECPELRFAYMVTTVLLSVLVKRLHLLSV EGQVIETKYELVTSSREEAWITVSKRY*NFIHLK SLLN*LRKTTI*KKSMLNPFINQYHFVI*TPIC T*FCKFGFLYIIFS*FIVHI*LTAQYIDHFNGK L*LSTFYFCFFTFYAIIFVFFFLV*ALKSMFLKK KLF

Frame 1

DRALLTPNFSQKAGLCFRQQPGPKSRAYSV*NR VHRNPRLAVEAGARRPQPRPAALRNPRPGSSAI HCSFLWRRPQASVTPPAARWFPGPAGCPRPHAMP *IGPG*GAAAGAADPRRGSLGGRTMLDFAIFAV TFLLALVGAVLYLYPASRQAAGIPGITPTEEKD GNLPDIVNSGSLHEFLVNLHERYGPVVSFWFGRR LVVSLGTVDVLKQHINPNKTSDPFETMLKSLLR YQSGGGSVSENHMRKKLYENGVTDSLKSNFALL LKLSEELLDKWLSYPETQHVPLSQHMLGFAMKSV TQMVMGSTFEDDQEVIRFQKNHGTPSCNWSLF* GTS*KNEKEGTSVNIFSLTP*YKGTLMTNRS*K TV*YFLWPVA**LQNCVPGQSVF*PPLKKFKKNY MKR*TKFLEMVLLLQRKLSSSDIVSMCFVKLFE LPN*LQFLPSFKILKEKLTDLLFLERPSSFMPL VWYFRILILGHLHTSLIQIGLMMN***KLFPHLD SQAHRSVQS*GLHIW*PQYFLVYW*RDCTYFLW RDRLLKQSMNW*HHQGKKLGSLSQRDIKILYI* NHC*ID*GKQPFKKNLC*ILL*TSITL*YKHLFV LNFVNLDFYISYFLNSLYTFDLLHSILIILMGN FSFLLFIFVFSLSMPLFLYSFS*CEL*NQCS*K RNYF

Frame 2

TEHY*LLTFLRKLGSAFASNPVRSRGHILSEIV CTEIPALRWRLALGGLSLGLLRSGTRAPAPRRS IALSSGAGRRPRSRPQRPVGFRVPRGAPAHTLC LKLGQAEALLLERPIRDVAPWAAEPCWTSRSSPL PSCWRWWEPCSTSIRLPDKLQEFQGLLQLKKKM VIFQIL*IVEVCMSSWLICMRDMGLWSPSGLAG ASWLVWALLMY*SSISIPIRHRTLLKPC*SHY*G INLVVAV*VKTT*GKNCMKMV*LIL*RVTLPSS *SFQKNY*INGSPTQRPSTCPSASICLVLL*SL LHRW*WVVHLKMIRKSFASRRIMAHPHATGVCFK EHHKRTKRKELQSTYFH*LLSTREP**PTDPRR QYDIFSGQLHNNCKIVYLGNLFFNHL*RSSKKI I*RDKPSFWKWSCYSREN*AAQILSACAL*NCSN CQTDSSFCPASRY*RKN*PIYYS*RDPRPLCPW CGTSGS*YLAISTQV*SRSV***ISNENFFLTW ILRHTGVSRVEVCIYGDHSTS*CIGEETAPTFCG GTGY*NKV*TGNIIKGRSLDHCLKEILKFYTFK IIVKLIEENNHLKKIYVESFYKPVSLCNINTYL YLIL*IWIFIYHIFLIHCTHLTYCTVY*SF*WET LAFYFLFLFFHFLCHYFCILFLSVSSKINVLEK EIIL

Sequence NM_177538

GGACAGAGCACTACTAACTCCTAACTTTTCTCAGAAAGCTGGGCTCTGCTTTCGCCAGCAACCCGGTCCGAAGTCGCGGGCATATTCTGTCTGAAATCGT GTGCACCGAAATCCCCGCCTTGCGGTGGAGGCTGGCGCTAGGCGGCCTCAGCCTCGGCCTGCTGCGCTCAGGAACCCGCGCCCCGGCTCCTCGGCGATCC ATTGCTCTTTCCTCTGGCGCCGGCCGCAGGCCTCGGTCACGCCCCCAGCGGCCCGTTGGTTTCCGGGTCCCGCGGGGTGCCCCCGCCCACACGCTATGCC TTAAATTGGGCCAGGCTGAGGCGCTGCTGCTGGAGCGGCCGATCCGAGACGTGGCTCCCTGGGCGGCAGAACCATGTTGGACTTCGCGATCTTCGCCGTT ACCTTCTTGCTGGCGTTGGTGGGAGCCGTGCTCTACCTCTATCCGGCTTCCAGACAAGCTGCAGGAATTCCAGGGATTACTCCAACTGAAGAAAAAGATG GTAATCTTCCAGATATTGTGAATAGTGGAAGTTTGCATGAGTTCCTGGTTAATTTGCATGAGAGATATGGGCCTGTGGTCTCCTTCTGGTTTGGCAGGCG CCTCGTGGTTAGTTTGGGCACTGTTGATGTACTGAAGCAGCATATCAATCCCAATAAGACATCGGACCCTTTTGAAACCATGCTGAAGTCATTATTAAGG TATCAATCTGGTGGTGGCAGTGTGAGTGAAAACCACATGAGGAAAAAATTGTATGAAAATGGTGTGACTGATTCTCTGAAGAGTAACTTTGCCCTCCTCC TAAAGCTTTCAGAAGAATTATTAGATAAATGGCTCTCCTACCCAGAGACCCAGCACGTGCCCCTCAGCCAGCATATGCTTGGTTTTGCTATGAAGTCTGT TACACAGATGGTAATGGGTAGTACATTTGAAGATGATCAGGAAGTCATTCGCTTCCAGAAGAATCATGGCACAGTTTGGTCTGAGATTGGAAAAGGCTTT CTAGATGGGTCACTTGATAAAAACATGACTCGGAAAAAACAATATGAAGATGCCCTCATGCAACTGGAGTCTGTTTTAAGGAACATCATAAAAGAACGAA AAGGAAGGAACTTCAGTCAACATATTTTCATTGACTCCTTAGTACAAGGGAACCTTAATGACCAACAGATCCTAGAAGACAGTATGATATTTTCTCTGGC CAGTTGCATAATAACTGCAAAATTGTGTACCTGGGCAATCTGTTTTTTAACCACCTCTGAAGAAGTTCAAAAAAAATTATATGAAGAGATAAACCAAGTT TTTGGAAATGGTCCTGTTACTCCAGAGAAAATTGAGCAGCTCAGATATTGTCAGCATGTGCTTTGTGAAACTGTTCGAACTGCCAAACTGACTCCAGTTT CTGCCCAGCTTCAAGATATTGAAGGAAAAATTGACCGATTTATTATTCCTAGAGAGACCCTCGTCCTTTATGCCCTTGGTGTGGTACTTCAGGATCCTAA TACTTGGCCATCTCCACACAAGTTTGATCCAGATCGGTTTGATGATGAATTAGTAATGAAAACTTTTTCCTCACTTGGATTCTCAGGCACACAGGAGTGT CCAGAGTTGAGGTTTGCATATATGGTGACCACAGTACTTCTTAGTGTATTGGTGAAGAGACTGCACCTACTTTCTGTGGAGGGACAGGTTATTGAAACAA AGTATGAACTGGTAACATCATCAAGGGAAGAAGCTTGGATCACTGTCTCAAAGAGATATTAAAATTTTATACATTTAAAATCATTGTTAAATTGATTGAG GAAAACAACCATTTAAAAAAAATCTATGTTGAATCCTTTTATAAACCAGTATCACTTTGTAATATAAACACCTATTTGTACTTAATTTTGTAAATTTGGA TTTTTATATATCATATTTTCTTAATTCATTGTACACATTTGACTTACTGCACAGTATATTGATCATTTTAATGGGAAACTTTAGCTTTCTACTTTTTATT TTTGTTTTTTCACTTTCTATGCCATTATTTTTGTATTCTTTTTCTTAGTGTGAGCTCTAAAATCAATGTTCTTGAAAAAGAAATTATTTTG

refseq_CYP20A1.F1 (forward primer)
refseq_CYP20A1.R1 (reverse primer)
Single exon skipping

Frame 0

GQSTTNS*LFSESWALLSPATRSEVAGIFCLKS CAPKSPPCGGGWR*AASASACCAQEPAPRLLGDP LLFPLAPAAGLGHAPSGPLVSGSRGVPPPTRYA LNWARLRRCCWSGRSETWLPGRQNHVGLRDLRR YLLAGVGGSRALPLSGFQTSCRNSRDYSN*RKRW *SSRYCE*WKFA*VPG*FA*EIWACGLLLVWQA PRG*FGHC*CTEAAYQSQ*DIGPF*NHAEVIIK VSIWWWQCE*KPHEEKIV*KWCD*FSEE*LCPPP KAFRRIIR*MALLPRDPARAPQPAYAWFCYEVC YTDGNG*YI*R*SGSHSLPEESWHSLV*DWKRL SRWVT**KHDSEKTI*RCPHATGVCFKEHHKRTK RKELQSTYFH*LLSTREP**PTDPRRQYDIFSG QLHNNCKIVYLGNLFFNHL*RSSKKII*RDKPS FWKWSCYSREN*AAQILSACAL*NCSNCQTDSSF CPASRY*RKN*PIYYS*RDPRPLCPWCGTSGS* YLAISTQV*SRSV***ISNENFFLTWILRHTGV SRVEVCIYGDHSTS*CIGEETAPTFCGGTGY*NK V*TGNIIKGRSLDHCLKEILKFYTFKIIVKLIE ENNHLKKIYVESFYKPVSLCNINTYLYLIL*IW IFIYHIFLIHCTHLTYCTVY*SF*WETLAFYFLF LFFHFLCHYFCILFLSVSSKINVLEKEIIL

Frame 1

DRALLTPNFSQKAGLCFRQQPGPKSRAYSV*NR VHRNPRLAVEAGARRPQPRPAALRNPRPGSSAI HCSFLWRRPQASVTPPAARWFPGPAGCPRPHAMP *IGPG*GAAAGAADPRRGSLGGRTMLDFAIFAV TFLLALVGAVLYLYPASRQAAGIPGITPTEEKD GNLPDIVNSGSLHEFLVNLHERYGPVVSFWFGRR LVVSLGTVDVLKQHINPNKTSDPFETMLKSLLR YQSGGGSVSENHMRKKLYENGVTDSLKSNFALL LKLSEELLDKWLSYPETQHVPLSQHMLGFAMKSV TQMVMGSTFEDDQEVIRFQKNHGTVWSEIGKGF LDGSLDKNMTRKKQYEDALMQLESVLRNIIKER KGRNFSQHIFIDSLVQGNLNDQQILEDSMIFSLA SCIITAKLCTWAICFLTTSEEVQKKLYEEINQV FGNGPVTPEKIEQLRYCQHVLCETVRTAKLTPV SAQLQDIEGKIDRFIIPRETLVLYALGVVLQDPN TWPSPHKFDPDRFDDELVMKTFSSLGFSGTQEC PELRFAYMVTTVLLSVLVKRLHLLSVEGQVIET KYELVTSSREEAWITVSKRY*NFIHLKSLLN*LR KTTI*KKSMLNPFINQYHFVI*TPICT*FCKFG FLYIIFS*FIVHI*LTAQYIDHFNGKL*LSTFY FCFFTFYAIIFVFFFLV*ALKSMFLKKKLF

Frame 2

TEHY*LLTFLRKLGSAFASNPVRSRGHILSEIV CTEIPALRWRLALGGLSLGLLRSGTRAPAPRRS IALSSGAGRRPRSRPQRPVGFRVPRGAPAHTLC LKLGQAEALLLERPIRDVAPWAAEPCWTSRSSPL PSCWRWWEPCSTSIRLPDKLQEFQGLLQLKKKM VIFQIL*IVEVCMSSWLICMRDMGLWSPSGLAG ASWLVWALLMY*SSISIPIRHRTLLKPC*SHY*G INLVVAV*VKTT*GKNCMKMV*LIL*RVTLPSS *SFQKNY*INGSPTQRPSTCPSASICLVLL*SL LHRW*WVVHLKMIRKSFASRRIMAQFGLRLEKAF *MGHLIKT*LGKNNMKMPSCNWSLF*GTS*KNE KEGTSVNIFSLTP*YKGTLMTNRS*KTV*YFLW PVA**LQNCVPGQSVF*PPLKKFKKNYMKR*TKF LEMVLLLQRKLSSSDIVSMCFVKLFELPN*LQF LPSFKILKEKLTDLLFLERPSSFMPLVWYFRIL ILGHLHTSLIQIGLMMN***KLFPHLDSQAHRSV QS*GLHIW*PQYFLVYW*RDCTYFLWRDRLLKQ SMNW*HHQGKKLGSLSQRDIKILYI*NHC*ID* GKQPFKKNLC*ILL*TSITL*YKHLFVLNFVNLD FYISYFLNSLYTFDLLHSILIILMGNFSFLLFI FVFSLSMPLFLYSFS*CEL*NQCS*KRNYF