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Sequences

Sequence NM_001005268

ACCTAAGCGATGAAATCCAAAGGTGAATCTTTTGGTTGGTGATTTTGATGATAGCCACAAAGCAAGCAGGCTGTATGGTAGCCTTGAAAACCAACAGGCC ACAATCACAGTGAAAACCTCAGGTTGGCAGCCACTGGAGGATACAAAATGAGTTTGGATCTCCTTCAATGCCCCATTCCCAGAGAACTGTCATTATTTGA CCTGCTGGTAGTTCCCTAAAGGACGTGCAAGGATGTTTTTATTTGACTGGACACTTGAAGCTTGCCCTGTATGAACAGCCCCCATCTTCCCATGCTTGTC AAAAATCAGAGGCAACTAACACTGCGGCTTCCTGAGGGAACTCCGGATGTGACTCCTGTTACATGAAAATGGAAGAGGTCAGAGTTGTCCAAGAGGGAGG CCAGCCACATCCAGGCAGGGCCCCCATGGTCAGAACCCTGCTAGAGCCATCAAGGTCCCAGATGAGTCTGGAAGTGTTAGTTGCAAAGATATGCATTTAC TTCTCTGGCTACAAAAAAGAATAGAAATGCACAAAGCAGAGCAGTGTGAAGAAGAAGAGGCGATGACCCCTAGACCGACCAAAGCCCGTGCTCCACTGCC CAGTGCCTATGTCCCACCACTGTCGCTGCCACCCTGCCCGAGAGAAAGGCTGAAGGGGATGCTAAAGGAGATAAAACCAAGGTTAAGTAGGAACTGCAGA GAAGATCCACAAGGTTGTCTGCTAAACCTGCTCCTCCAAAGCCACAGCCGAAGCCCAGAAAGGCCTCTGCAAAGAAGAGAGAGAAGGTACCTACAGAGAA GAAGGGAAAAGCTGATGCTGGCAAGGAGGGGAATAACCCTGCAGAAAATGGAGATGCCAAAACAGACTAGGCACAGAAAGCTAAAGGTGCTGGAGATGCC AAGTGAAGTGTGTGCATTTTTGATAACTGTGTACTTCTGGTGACTGTACAGTTTGAAATACTATTTTTTAATCAAGTTTTATAAAAATGCAGATTTTGTT TAGCTTTTTTTTTTTTTTTTAAGCTATGTTAGTACACAGAATACTTCATTGTTGTTTTGGGGAGAAGGGCCATATGTCACTAATAGAAAGCCCCTGAAGC TGGACTGATGTGGGGAAAACACCTTTTCCTTCTGGTTTTGAGAGACTTCCTCTTGGGTCCCAGGAAGAGGGATTCCCTGACTTTGATACACATGGCCACC TTGGTATAAAAGCCTTGTGGTATGGAAAAACAAATTCGTTTTTTATGTCCTCTTCTCTGTTTCTACCTTTCAGAATGGACTTAAATCCCTTAAACCCAGA CATCTTCTGGGACCTGACCCCCAATAATTGGTTACCAGTGTGTCAGGCAATCTGGACTTTGCCCTCAAAAGAGCAGTGGTTCCTGTTTCTAGGTTGTGGA TCTTCAGATAAATTCTGTCATTTTCCTTTCACTTCCTGAAAGCCAGGGTCGGTTCATGAAAAGCTATGAAACAGCATGCTAAATGTGAAATGTCAACCCT CACTAAACTTTGCCGGTTCAGAGCATCAGATGAAGACTTCATTGGGTTTTACAGTGGCTTTCTGATTTTTGGTAGTCCATTGAAGAAGGGAGTTTGAAAG TTGTTGTATACTGTTAACGATTGTCTGCCCATGTCCTGCCCAAAATACCATGATTCTTTATGGAAATTATCTTTAATAAAGCTGGATACAGTTTGGCTTG GG

refseq_C15orf21.F6 (forward primer)
refseq_C15orf21.R7 (reverse primer)
Single exon skipping

Frame 0

T*AMKSKGESFGW*F***PQSKQAVW*P*KPTG HNHSENLRLAATGGYKMSLDLLQCPIPRELSLFD LLVVP*RTCKDVFI*LDT*SLPCMNSPHLPMLV KNQRQLTLRLPEGTPDVTPVT*KWKRSELSKRE ASHIQAGPPWSEPC*SHQGPR*VWKC*LQRYAFT SLATKKNRNAQSRAV*RRRGDDP*TDQSPCSTA QCLCPTTVAATLPERKAEGDAKGDKTKVK*ELQ RRSTRLSAKPAPPKPQPKPRKASAKKREKVPTEK KGKADAGKEGNNPAENGDAKTD*AQKAKGAGDA K*SVCIFDNCVLLVTVQFEILFFNQVL*KCRFC LAFFFFFKLC*YTEYFIVVLGRRAICH**KAPEA GLMWGKHLFLLVLRDFLLGPRKRDSLTLIHMAT LV*KPCGMEKQIRFLCPLLCFYLSEWT*IP*TQ TSSGT*PPIIGYQCVRQSGLCPQKSSGSCF*VVD LQINSVIFLSLPESQGRFMKSYETAC*M*NVNP H*TLPVQSIR*RLHWVLQWLSDFW*SIEEGSLK VVVYC*RLSAHVLPKIP*FFMEIIFNKAGYSLAW

Frame 1

PKR*NPKVNLLVGDFDDSHKASRLYGSLENQQA TITVKTSGWQPLEDTK*VWISFNAPFPENCHYL TCW*FPKGRARMFLFDWTLEACPV*TAPIFPCLS KIRGN*HCGFLRELRM*LLLHENGRGQSCPRGR PATSRQGPHGQNPARAIKVPDESGSVSCKDMHL LLWLQKRIEMHKAEQCEEEEAMTPRPTKARAPLP SAYVPPLSLPPCPRERLKGMLKEIKPRLSRNCR EDPQGCLLNLLLQSHSRSPERPLQRRERRYLQR RREKLMLARRGITLQKMEMPKQTRHRKLKVLEMP SEVCAFLITVYFW*LYSLKYYFLIKFYKNADFV *LFFFFLSYVSTQNTSLLFWGEGPYVTNRKPLK LD*CGENTFSFWF*ETSSWVPGRGIP*L*YTWPP WYKSLVVWKNKFVFYVLFSVSTFQNGLKSLKPR HLLGPDPQ*LVTSVSGNLDFALKRAVVPVSRLW IFR*ILSFSFHFLKARVGS*KAMKQHAKCEMSTL TKLCRFRASDEDFIGFYSGFLIFGSPLKKGV*K LLYTVNDCLPMSCPKYHDSLWKLSLIKLDTVWL G

Frame 2

LSDEIQR*IFWLVILMIATKQAGCMVALKTNRP QSQ*KPQVGSHWRIQNEFGSPSMPHSQRTVII* PAGSSLKDVQGCFYLTGHLKLALYEQPPSSHAC QKSEATNTAAS*GNSGCDSCYMKMEEVRVVQEGG QPHPGRAPMVRTLLEPSRSQMSLEVLVAKICIY FSGYKKE*KCTKQSSVKKKRR*PLDRPKPVLHC PVPMSHHCRCHPAREKG*RGC*RR*NQG*VGTAE KIHKVVC*TCSSKATAEAQKGLCKEEREGTYRE EGKS*CWQGGE*PCRKWRCQNRLGTES*RCWRC QVKCVHF**LCTSGDCTV*NTIF*SSFIKMQILF SFFFFF*AMLVHRILHCCFGEKGHMSLIESP*S WTDVGKTPFPSGFERLPLGSQEEGFPDFDTHGH LGIKALWYGKTNSFFMSSSLFLPFRMDLNPLNPD IFWDLTPNNWLPVCQAIWTLPSKEQWFLFLGCG SSDKFCHFPFTS*KPGSVHEKL*NSMLNVKCQP SLNFAGSEHQMKTSLGFTVAF*FLVVH*RREFES CCILLTIVCPCPAQNTMILYGNYL**SWIQFGL

Sequence NM_001005266

ACCTAAGCGATGAAATCCAAAGGTGAATCTTTTGGTTGGTGATTTTGATGATAGCCACAAAGCAAGCAGGCTGTATGGTAGCCTTGAAAACCAACAGGCC ACAATCACAGTGAAAACCTCAGGTTGGCAGCCACTGGAGGATACAAAATGAGTTTGGATCTCCTTCAATGCCCCATTCCCAGAGAACTGTCATTATTTGA CCTGCTGGTAGTTCCCTAAAGGACGTGCAAGGATGTTTTTATTTGACTGGACACTTGAAGCTTGCCCTGTATGAACAGCCCCCATCTTCCCATGCTTGTC AAAAATCAGAGGCAACTAACACTGCGGCTTCCTGAGAGTCTCACTCTGTCACCCAGGCTGGAGAGCAATTGCACAAACACTTGGCTCACTGCAGCCTCAA CTTCTTGTCCTCAAGTGATCCTCCCACCTTAGCCTCCTAAGTAGCTGGGACCACAGGGAACTCCGGATGTGACTCCTGTTACATGAAAATGGAAGAGGTC AGAGTTGTCCAAGAGGGAGGCCAGCCACATCCAGGCAGGGCCCCCATGGTCAGAACCCTGCTAGAGCCATCAAGGTCCCAGAGCTCCCAAGATGGTGGCA AGCCTTTTGTTCTCTGACCAGAGGTTCCTGGCCTCACGGATTCCAAGGAATGGAACCTTGGGCCATGCGTGCACTTGATTTTGCAGATGAGTCTGGAAGT GTTAGTTGCAAAGATATGCATTTACTTCTCTGGCTACAAAAAAGAATAGAAATGCACAAAGCAGAGCAGTGTGAAGAAGAAGAGGCGATGACCCCTAGAC CGACCAAAGCCCGTGCTCCACTGCCCAGTGCCTATGTCCCACCACTGTCGCTGCCACCCTGCCCGAGAGAAAGGCTGAAGGGGATGCTAAAGGAGATAAA ACCAAGGTTAAGTAGGAACTGCAGAGAAGATCCACAAGGTTGTCTGCTAAACCTGCTCCTCCAAAGCCACAGCCGAAGCCCAGAAAGGCCTCTGCAAAGA AGAGAGAGAAGGTACCTACAGAGAAGAAGGGAAAAGCTGATGCTGGCAAGGAGGGGAATAACCCTGCAGAAAATGGAGATGCCAAAACAGACTAGGCACA GAAAGCTAAAGGTGCTGGAGATGCCAAGTGAAGTGTGTGCATTTTTGATAACTGTGTACTTCTGGTGACTGTACAGTTTGAAATACTATTTTTTAATCAA GTTTTATAAAAATGCAGATTTTGTTTAGCTTTTTTTTTTTTTTTTAAGCTATGTTAGTACACAGAATACTTCATTGTTGTTTTGGGGAGAAGGGCCATAT GTCACTAATAGAAAGCCCCTGAAGCTGGACTGATGTGGGGAAAACACCTTTTCCTTCTGGTTTTGAGAGACTTCCTCTTGGGTCCCAGGAAGAGGGATTC CCTGACTTTGATACACATGGCCACCTTGGTATAAAAGCCTTGTGGTATGGAAAAACAAATTCGTTTTTTATGTCCTCTTCTCTGTTTCTACCTTTCAGAA TGGACTTAAATCCCTTAAACCCAGACATCTTCTGGGACCTGACCCCCAATAATTGGTTACCAGTGTGTCAGGCAATCTGGACTTTGCCCTCAAAAGAGCA GTGGTTCCTGTTTCTAGGTTGTGGATCTTCAGATAAATTCTGTCATTTTCCTTTCACTTCCTGAAAGCCAGGGTCGGTTCATGAAAAGCTATGAAACAGC ATGCTAAATGTGAAATGTCAACCCTCACTAAACTTTGCCGGTTCAGAGCATCAGATGAAGACTTCATTGGGTTTTACAGTGGCTTTCTGATTTTTGGTAG TCCATTGAAGAAGGGAGTTTGAAAGTTGTTGTATACTGTTAACGATTGTCTGCCCATGTCCTGCCCAAAATACCATGATTCTTTATGGAAATTATCTTTA ATAAAGCTGGATACAGTTTGGCTTGGG

refseq_C15orf21.F6 (forward primer)
refseq_C15orf21.R7 (reverse primer)
Single exon skipping

Frame 0

T*AMKSKGESFGW*F***PQSKQAVW*P*KPTG HNHSENLRLAATGGYKMSLDLLQCPIPRELSLFD LLVVP*RTCKDVFI*LDT*SLPCMNSPHLPMLV KNQRQLTLRLPESLTLSPRLESNCTNTWLTAAS TSCPQVILPP*PPK*LGPQGTPDVTPVT*KWKRS ELSKREASHIQAGPPWSEPC*SHQGPRAPKMVA SLLFSDQRFLASRIPRNGTLGHACT*FCR*VWK C*LQRYAFTSLATKKNRNAQSRAV*RRRGDDP*T DQSPCSTAQCLCPTTVAATLPERKAEGDAKGDK TKVK*ELQRRSTRLSAKPAPPKPQPKPRKASAK KREKVPTEKKGKADAGKEGNNPAENGDAKTD*AQ KAKGAGDAK*SVCIFDNCVLLVTVQFEILFFNQ VL*KCRFCLAFFFFFKLC*YTEYFIVVLGRRAI CH**KAPEAGLMWGKHLFLLVLRDFLLGPRKRDS LTLIHMATLV*KPCGMEKQIRFLCPLLCFYLSE WT*IP*TQTSSGT*PPIIGYQCVRQSGLCPQKS SGSCF*VVDLQINSVIFLSLPESQGRFMKSYETA C*M*NVNPH*TLPVQSIR*RLHWVLQWLSDFW* SIEEGSLKVVVYC*RLSAHVLPKIP*FFMEIIF NKAGYSLAW

Frame 1

PKR*NPKVNLLVGDFDDSHKASRLYGSLENQQA TITVKTSGWQPLEDTK*VWISFNAPFPENCHYL TCW*FPKGRARMFLFDWTLEACPV*TAPIFPCLS KIRGN*HCGFLRVSLCHPGWRAIAQTLGSLQPQ LLVLK*SSHLSLLSSWDHRELRM*LLLHENGRG QSCPRGRPATSRQGPHGQNPARAIKVPELPRWWQ AFCSLTRGSWPHGFQGMEPWAMRALDFADESGS VSCKDMHLLLWLQKRIEMHKAEQCEEEEAMTPR PTKARAPLPSAYVPPLSLPPCPRERLKGMLKEIK PRLSRNCREDPQGCLLNLLLQSHSRSPERPLQR RERRYLQRRREKLMLARRGITLQKMEMPKQTRH RKLKVLEMPSEVCAFLITVYFW*LYSLKYYFLIK FYKNADFV*LFFFFLSYVSTQNTSLLFWGEGPY VTNRKPLKLD*CGENTFSFWF*ETSSWVPGRGI P*L*YTWPPWYKSLVVWKNKFVFYVLFSVSTFQN GLKSLKPRHLLGPDPQ*LVTSVSGNLDFALKRA VVPVSRLWIFR*ILSFSFHFLKARVGS*KAMKQ HAKCEMSTLTKLCRFRASDEDFIGFYSGFLIFGS PLKKGV*KLLYTVNDCLPMSCPKYHDSLWKLSL IKLDTVWLG

Frame 2

LSDEIQR*IFWLVILMIATKQAGCMVALKTNRP QSQ*KPQVGSHWRIQNEFGSPSMPHSQRTVII* PAGSSLKDVQGCFYLTGHLKLALYEQPPSSHAC QKSEATNTAAS*ESHSVTQAGEQLHKHLAHCSLN FLSSSDPPTLAS*VAGTTGNSGCDSCYMKMEEV RVVQEGGQPHPGRAPMVRTLLEPSRSQSSQDGG KPFVL*PEVPGLTDSKEWNLGPCVHLILQMSLEV LVAKICIYFSGYKKE*KCTKQSSVKKKRR*PLD RPKPVLHCPVPMSHHCRCHPAREKG*RGC*RR* NQG*VGTAEKIHKVVC*TCSSKATAEAQKGLCKE EREGTYREEGKS*CWQGGE*PCRKWRCQNRLGT ES*RCWRCQVKCVHF**LCTSGDCTV*NTIF*S SFIKMQILFSFFFFF*AMLVHRILHCCFGEKGHM SLIESP*SWTDVGKTPFPSGFERLPLGSQEEGF PDFDTHGHLGIKALWYGKTNSFFMSSSLFLPFR MDLNPLNPDIFWDLTPNNWLPVCQAIWTLPSKEQ WFLFLGCGSSDKFCHFPFTS*KPGSVHEKL*NS MLNVKCQPSLNFAGSEHQMKTSLGFTVAF*FLV VH*RREFESCCILLTIVCPCPAQNTMILYGNYL* *SWIQFGL

Sequence NM_001005267

ACCTAAGCGATGAAATCCAAAGGTGAATCTTTTGGTTGGTGATTTTGATGATAGCCACAAAGCAAGCAGGCTGTATGGTAGCCTTGAAAACCAACAGGCC ACAATCACAGTGAAAACCTCAGGTTGGCAGCCACTGGAGGATACAAAATGAGTTTGGATCTCCTTCAATGCCCCATTCCCAGAGAACTGTCATTATTTGA CCTGCTGGTAGTTCCCTAAAGGACGTGCAAGGATGTTTTTATTTGACTGGACACTTGAAGCTTGCCCTGTATGAACAGCCCCCATCTTCCCATGCTTGTC AAAAATCAGAGGCAACTAACACTGCGGCTTCCTGAGAGCTCCCAAGATGGTGGCAAGCCTTTTGTTCTCTGACCAGAGGTTCCTGGCCTCACGGATTCCA AGGAATGGAACCTTGGGCCATGCGTGCACTTGATTTTGCAGATGAGTCTGGAAGTGTTAGTTGCAAAGATATGCATTTACTTCTCTGGCTACAAAAAAGA ATAGAAATGCACAAAGCAGAGCAGTGTGAAGAAGAAGAGGCGATGACCCCTAGACCGACCAAAGCCCGTGCTCCACTGCCCAGTGCCTATGTCCCACCAC TGTCGCTGCCACCCTGCCCGAGAGAAAGGCTGAAGGGGATGCTAAAGGAGATAAAACCAAGGTTAAGTAGGAACTGCAGAGAAGATCCACAAGGTTGTCT GCTAAACCTGCTCCTCCAAAGCCACAGCCGAAGCCCAGAAAGGCCTCTGCAAAGAAGAGAGAGAAGGTACCTACAGAGAAGAAGGGAAAAGCTGATGCTG GCAAGGAGGGGAATAACCCTGCAGAAAATGGAGATGCCAAAACAGACTAGGCACAGAAAGCTAAAGGTGCTGGAGATGCCAAGTGAAGTGTGTGCATTTT TGATAACTGTGTACTTCTGGTGACTGTACAGTTTGAAATACTATTTTTTAATCAAGTTTTATAAAAATGCAGATTTTGTTTAGCTTTTTTTTTTTTTTTT AAGCTATGTTAGTACACAGAATACTTCATTGTTGTTTTGGGGAGAAGGGCCATATGTCACTAATAGAAAGCCCCTGAAGCTGGACTGATGTGGGGAAAAC ACCTTTTCCTTCTGGTTTTGAGAGACTTCCTCTTGGGTCCCAGGAAGAGGGATTCCCTGACTTTGATACACATGGCCACCTTGGTATAAAAGCCTTGTGG TATGGAAAAACAAATTCGTTTTTTATGTCCTCTTCTCTGTTTCTACCTTTCAGAATGGACTTAAATCCCTTAAACCCAGACATCTTCTGGGACCTGACCC CCAATAATTGGTTACCAGTGTGTCAGGCAATCTGGACTTTGCCCTCAAAAGAGCAGTGGTTCCTGTTTCTAGGTTGTGGATCTTCAGATAAATTCTGTCA TTTTCCTTTCACTTCCTGAAAGCCAGGGTCGGTTCATGAAAAGCTATGAAACAGCATGCTAAATGTGAAATGTCAACCCTCACTAAACTTTGCCGGTTCA GAGCATCAGATGAAGACTTCATTGGGTTTTACAGTGGCTTTCTGATTTTTGGTAGTCCATTGAAGAAGGGAGTTTGAAAGTTGTTGTATACTGTTAACGA TTGTCTGCCCATGTCCTGCCCAAAATACCATGATTCTTTATGGAAATTATCTTTAATAAAGCTGGATACAGTTTGGCTTGGG

refseq_C15orf21.F6 (forward primer)
refseq_C15orf21.R7 (reverse primer)
Single exon skipping

Frame 0

T*AMKSKGESFGW*F***PQSKQAVW*P*KPTG HNHSENLRLAATGGYKMSLDLLQCPIPRELSLFD LLVVP*RTCKDVFI*LDT*SLPCMNSPHLPMLV KNQRQLTLRLPESSQDGGKPFVL*PEVPGLTDS KEWNLGPCVHLILQMSLEVLVAKICIYFSGYKKE *KCTKQSSVKKKRR*PLDRPKPVLHCPVPMSHH CRCHPAREKG*RGC*RR*NQG*VGTAEKIHKVV C*TCSSKATAEAQKGLCKEEREGTYREEGKS*CW QGGE*PCRKWRCQNRLGTES*RCWRCQVKCVHF **LCTSGDCTV*NTIF*SSFIKMQILFSFFFFF *AMLVHRILHCCFGEKGHMSLIESP*SWTDVGKT PFPSGFERLPLGSQEEGFPDFDTHGHLGIKALW YGKTNSFFMSSSLFLPFRMDLNPLNPDIFWDLT PNNWLPVCQAIWTLPSKEQWFLFLGCGSSDKFCH FPFTS*KPGSVHEKL*NSMLNVKCQPSLNFAGS EHQMKTSLGFTVAF*FLVVH*RREFESCCILLT IVCPCPAQNTMILYGNYL**SWIQFGL

Frame 1

PKR*NPKVNLLVGDFDDSHKASRLYGSLENQQA TITVKTSGWQPLEDTK*VWISFNAPFPENCHYL TCW*FPKGRARMFLFDWTLEACPV*TAPIFPCLS KIRGN*HCGFLRAPKMVASLLFSDQRFLASRIP RNGTLGHACT*FCR*VWKC*LQRYAFTSLATKK NRNAQSRAV*RRRGDDP*TDQSPCSTAQCLCPTT VAATLPERKAEGDAKGDKTKVK*ELQRRSTRLS AKPAPPKPQPKPRKASAKKREKVPTEKKGKADA GKEGNNPAENGDAKTD*AQKAKGAGDAK*SVCIF DNCVLLVTVQFEILFFNQVL*KCRFCLAFFFFF KLC*YTEYFIVVLGRRAICH**KAPEAGLMWGK HLFLLVLRDFLLGPRKRDSLTLIHMATLV*KPCG MEKQIRFLCPLLCFYLSEWT*IP*TQTSSGT*P PIIGYQCVRQSGLCPQKSSGSCF*VVDLQINSV IFLSLPESQGRFMKSYETAC*M*NVNPH*TLPVQ SIR*RLHWVLQWLSDFW*SIEEGSLKVVVYC*R LSAHVLPKIP*FFMEIIFNKAGYSLAW

Frame 2

LSDEIQR*IFWLVILMIATKQAGCMVALKTNRP QSQ*KPQVGSHWRIQNEFGSPSMPHSQRTVII* PAGSSLKDVQGCFYLTGHLKLALYEQPPSSHAC QKSEATNTAAS*ELPRWWQAFCSLTRGSWPHGFQ GMEPWAMRALDFADESGSVSCKDMHLLLWLQKR IEMHKAEQCEEEEAMTPRPTKARAPLPSAYVPP LSLPPCPRERLKGMLKEIKPRLSRNCREDPQGCL LNLLLQSHSRSPERPLQRRERRYLQRRREKLML ARRGITLQKMEMPKQTRHRKLKVLEMPSEVCAF LITVYFW*LYSLKYYFLIKFYKNADFV*LFFFFL SYVSTQNTSLLFWGEGPYVTNRKPLKLD*CGEN TFSFWF*ETSSWVPGRGIP*L*YTWPPWYKSLV VWKNKFVFYVLFSVSTFQNGLKSLKPRHLLGPDP Q*LVTSVSGNLDFALKRAVVPVSRLWIFR*ILS FSFHFLKARVGS*KAMKQHAKCEMSTLTKLCRF RASDEDFIGFYSGFLIFGSPLKKGV*KLLYTVND CLPMSCPKYHDSLWKLSLIKLDTVWLG

Sequence NM_001005269

ACCTAAGCGATGAAATCCAAAGGTGAATCTTTTGGTTGGTGATTTTGATGATAGCCACAAAGCAAGCAGGCTGTATGGTAGCCTTGAAAACCAACAGGCC ACAATCACAGTGAAAACCTCAGGTTGGCAGCCACTGGAGGATACAAAATGAGTTTGGATCTCCTTCAATGCCCCATTCCCAGAGAACTGTCATTATTTGA CCTGCTGGTAGTTCCCTAAAGGACGTGCAAGGATGTTTTTATTTGACTGGACACTTGAAGCTTGCCCTGTATGAACAGCCCCCATCTTCCCATGCTTGTC AAAAATCAGAGGCAACTAACACTGCGGCTTCCTGAGAGTCTCACTCTGTCACCCAGGCTGGAGAGCAATTGCACAAACACTTGGCTCACTGCAGCCTCAA CTTCTTGTCCTCAAGTGATCCTCCCACCTTAGCCTCCTAAGTAGCTGGGACCACAGATGAGTCTGGAAGTGTTAGTTGCAAAGATATGCATTTACTTCTC TGGCTACAAAAAAGAATAGAAATGCACAAAGCAGAGCAGTGTGAAGAAGAAGAGGCGATGACCCCTAGACCGACCAAAGCCCGTGCTCCACTGCCCAGTG CCTATGTCCCACCACTGTCGCTGCCACCCTGCCCGAGAGAAAGGCTGAAGGGGATGCTAAAGGAGATAAAACCAAGGTTAAGTAGGAACTGCAGAGAAGA TCCACAAGGTTGTCTGCTAAACCTGCTCCTCCAAAGCCACAGCCGAAGCCCAGAAAGGCCTCTGCAAAGAAGAGAGAGAAGGTACCTACAGAGAAGAAGG GAAAAGCTGATGCTGGCAAGGAGGGGAATAACCCTGCAGAAAATGGAGATGCCAAAACAGACTAGGCACAGAAAGCTAAAGGTGCTGGAGATGCCAAGTG AAGTGTGTGCATTTTTGATAACTGTGTACTTCTGGTGACTGTACAGTTTGAAATACTATTTTTTAATCAAGTTTTATAAAAATGCAGATTTTGTTTAGCT TTTTTTTTTTTTTTTAAGCTATGTTAGTACACAGAATACTTCATTGTTGTTTTGGGGAGAAGGGCCATATGTCACTAATAGAAAGCCCCTGAAGCTGGAC TGATGTGGGGAAAACACCTTTTCCTTCTGGTTTTGAGAGACTTCCTCTTGGGTCCCAGGAAGAGGGATTCCCTGACTTTGATACACATGGCCACCTTGGT ATAAAAGCCTTGTGGTATGGAAAAACAAATTCGTTTTTTATGTCCTCTTCTCTGTTTCTACCTTTCAGAATGGACTTAAATCCCTTAAACCCAGACATCT TCTGGGACCTGACCCCCAATAATTGGTTACCAGTGTGTCAGGCAATCTGGACTTTGCCCTCAAAAGAGCAGTGGTTCCTGTTTCTAGGTTGTGGATCTTC AGATAAATTCTGTCATTTTCCTTTCACTTCCTGAAAGCCAGGGTCGGTTCATGAAAAGCTATGAAACAGCATGCTAAATGTGAAATGTCAACCCTCACTA AACTTTGCCGGTTCAGAGCATCAGATGAAGACTTCATTGGGTTTTACAGTGGCTTTCTGATTTTTGGTAGTCCATTGAAGAAGGGAGTTTGAAAGTTGTT GTATACTGTTAACGATTGTCTGCCCATGTCCTGCCCAAAATACCATGATTCTTTATGGAAATTATCTTTAATAAAGCTGGATACAGTTTGGCTTGGG

refseq_C15orf21.F6 (forward primer)
refseq_C15orf21.R7 (reverse primer)
Single exon skipping

Frame 0

T*AMKSKGESFGW*F***PQSKQAVW*P*KPTG HNHSENLRLAATGGYKMSLDLLQCPIPRELSLFD LLVVP*RTCKDVFI*LDT*SLPCMNSPHLPMLV KNQRQLTLRLPESLTLSPRLESNCTNTWLTAAS TSCPQVILPP*PPK*LGPQMSLEVLVAKICIYFS GYKKE*KCTKQSSVKKKRR*PLDRPKPVLHCPV PMSHHCRCHPAREKG*RGC*RR*NQG*VGTAEK IHKVVC*TCSSKATAEAQKGLCKEEREGTYREEG KS*CWQGGE*PCRKWRCQNRLGTES*RCWRCQV KCVHF**LCTSGDCTV*NTIF*SSFIKMQILFS FFFFF*AMLVHRILHCCFGEKGHMSLIESP*SWT DVGKTPFPSGFERLPLGSQEEGFPDFDTHGHLG IKALWYGKTNSFFMSSSLFLPFRMDLNPLNPDI FWDLTPNNWLPVCQAIWTLPSKEQWFLFLGCGSS DKFCHFPFTS*KPGSVHEKL*NSMLNVKCQPSL NFAGSEHQMKTSLGFTVAF*FLVVH*RREFESC CILLTIVCPCPAQNTMILYGNYL**SWIQFGL

Frame 1

PKR*NPKVNLLVGDFDDSHKASRLYGSLENQQA TITVKTSGWQPLEDTK*VWISFNAPFPENCHYL TCW*FPKGRARMFLFDWTLEACPV*TAPIFPCLS KIRGN*HCGFLRVSLCHPGWRAIAQTLGSLQPQ LLVLK*SSHLSLLSSWDHR*VWKC*LQRYAFTS LATKKNRNAQSRAV*RRRGDDP*TDQSPCSTAQC LCPTTVAATLPERKAEGDAKGDKTKVK*ELQRR STRLSAKPAPPKPQPKPRKASAKKREKVPTEKK GKADAGKEGNNPAENGDAKTD*AQKAKGAGDAK* SVCIFDNCVLLVTVQFEILFFNQVL*KCRFCLA FFFFFKLC*YTEYFIVVLGRRAICH**KAPEAG LMWGKHLFLLVLRDFLLGPRKRDSLTLIHMATLV *KPCGMEKQIRFLCPLLCFYLSEWT*IP*TQTS SGT*PPIIGYQCVRQSGLCPQKSSGSCF*VVDL QINSVIFLSLPESQGRFMKSYETAC*M*NVNPH* TLPVQSIR*RLHWVLQWLSDFW*SIEEGSLKVV VYC*RLSAHVLPKIP*FFMEIIFNKAGYSLAW

Frame 2

LSDEIQR*IFWLVILMIATKQAGCMVALKTNRP QSQ*KPQVGSHWRIQNEFGSPSMPHSQRTVII* PAGSSLKDVQGCFYLTGHLKLALYEQPPSSHAC QKSEATNTAAS*ESHSVTQAGEQLHKHLAHCSLN FLSSSDPPTLAS*VAGTTDESGSVSCKDMHLLL WLQKRIEMHKAEQCEEEEAMTPRPTKARAPLPS AYVPPLSLPPCPRERLKGMLKEIKPRLSRNCRED PQGCLLNLLLQSHSRSPERPLQRRERRYLQRRR EKLMLARRGITLQKMEMPKQTRHRKLKVLEMPS EVCAFLITVYFW*LYSLKYYFLIKFYKNADFV*L FFFFLSYVSTQNTSLLFWGEGPYVTNRKPLKLD *CGENTFSFWF*ETSSWVPGRGIP*L*YTWPPW YKSLVVWKNKFVFYVLFSVSTFQNGLKSLKPRHL LGPDPQ*LVTSVSGNLDFALKRAVVPVSRLWIF R*ILSFSFHFLKARVGS*KAMKQHAKCEMSTLT KLCRFRASDEDFIGFYSGFLIFGSPLKKGV*KLL YTVNDCLPMSCPKYHDSLWKLSLIKLDTVWLG

Sequence NM_173609

ACCTAAGCGATGAAATCCAAAGGTGAATCTTTTGGTTGGTGATTTTGATGATAGCCACAAAGCAAGCAGGGTAATGCTGATGCTTCAAGTCTTGGAACCA CACTTCGAGAATCACTGTTATAATGTACAGCAGAACTGCATGATCAGCTGTATGGTAGCCTTGAAAACCAACAGGCCACAATCACAGTGAAAACCTCAGG TTGGCAGCCACTGGAGGATACAAAATGAGTTTGGATCTCCTTCAATGCCCCATTCCCAGAGAACTGTCATTATTTGACCTGCTGGTAGTTCCCTAAAGGA CGTGCAAGGATGTTTTTATTTGACTGGACACTTGAAGCTTGCCCTGTATGAACAGCCCCCATCTTCCCATGCTTGTCAAAAATCAGAGGCAACTAACACT GCGGCTTCCTGAGAGTCTCACTCTGTCACCCAGGCTGGAGAGCAATTGCACAAACACTTGGCTCACTGCAGCCTCAACTTCTTGTCCTCAAGTGATCCTC CCACCTTAGCCTCCTAAGTAGCTGGGACCACAGTGAGGTCAAAAAATGTTTGAAAGCCACTGAGATAGAAGGACCAGCCACTTTGAACGCCTTAGAGGCA AAAAGAGCTTTGTGAGTTGTAAGAGGAAAAAGAAGACCAGCATGGCTGAAGCGAGCCCTGGGGAGGAGTGTTATGAAAGAAGATGAGTCTGGAAGTGTTA GTTGCAAAGATATGCATTTACTTCTCTGGCTACAAAAAAGAATAGAAATGCACAAAGCAGAGCAGTGTGAAGAAGAAGAGGCGATGACCCCTAGACCGAC CAAAGCCCGTGCTCCACTGCCCAGTGCCTATGTCCCACCACTGTCGCTGCCACCCTGCCCGAGAGAAAGGCTGAAGGGGATGCTAAAGGAGATAAAACCA AGGTTAAGTAGGAACTGCAGAGAAGATCCACAAGGTTGTCTGCTAAACCTGCTCCTCCAAAGCCACAGCCGAAGCCCAGAAAGGCCTCTGCAAAGAAGAG AGAGAAGGTACCTACAGAGAAGAAGGGAAAAGCTGATGCTGGCAAGGAGGGGAATAACCCTGCAGAAAATGGAGATGCCAAAACAGACTAGGCACAGAAA GCTAAAGGTGCTGGAGATGCCAAGTGAAGTGTGTGCATTTTTGATAACTGTGTACTTCTGGTGACTGTACAGTTTGAAATACTATTTTTTAATCAAGTTT TATAAAAATGCAGATTTTGTTTAGCTTTTTTTTTTTTTTTTAAGCTATGTTAGTACACAGAATACTTCATTGTTGTTTTGGGGAGAAGGGCCATATGTCA CTAATAGAAAGCCCCTGAAGCTGGACTGATGTGGGGAAAACACCTTTTCCTTCTGGTTTTGAGAGACTTCCTCTTGGGTCCCAGGAAGAGGGATTCCCTG ACTTTGATACACATGGCCACCTTGGTATAAAAGCCTTGTGGTATGGAAAAACAAATTCGTTTTTTATGTCCTCTTCTCTGTTTCTACCTTTCAGAATGGA CTTAAATCCCTTAAACCCAGACATCTTCTGGGACCTGACCCCCAATAATTGGTTACCAGTGTGTCAGGCAATCTGGACTTTGCCCTCAAAAGAGCAGTGG TTCCTGTTTCTAGGTTGTGGATCTTCAGATAAATTCTGTCATTTTCCTTTCACTTCCTGAAAGCCAGGGTCGGTTCATGAAAAGCTATGAAACAGCATGC TAAATGTGAAATGTCAACCCTCACTAAACTTTGCCGGTTCAGAGCATCAGATGAAGACTTCATTGGGTTTTACAGTGGCTTTCTGATTTTTGGTAGTCCA TTGAAGAAGGGAGTTTGAAAGTTGTTGTATACTGTTAACGATTGTCTGCCCATGTCCTGCCCAAAATACCATGATTCTTTATGGAAATTATCTTTAATAA AGCTGGATACAGTTTGGCTTGGG

refseq_C15orf21.F6 (forward primer)
refseq_C15orf21.R7 (reverse primer)
Single exon skipping

Frame 0

T*AMKSKGESFGW*F***PQSKQGNADASSLGT TLRESLL*CTAELHDQLYGSLENQQATITVKTSG WQPLEDTK*VWISFNAPFPENCHYLTCW*FPKG RARMFLFDWTLEACPV*TAPIFPCLSKIRGN*H CGFLRVSLCHPGWRAIAQTLGSLQPQLLVLK*SS HLSLLSSWDHSEVKKCLKATEIEGPATLNALEA KRAL*VVRGKRRPAWLKRALGRSVMKEDESGSV SCKDMHLLLWLQKRIEMHKAEQCEEEEAMTPRPT KARAPLPSAYVPPLSLPPCPRERLKGMLKEIKP RLSRNCREDPQGCLLNLLLQSHSRSPERPLQRR ERRYLQRRREKLMLARRGITLQKMEMPKQTRHRK LKVLEMPSEVCAFLITVYFW*LYSLKYYFLIKF YKNADFV*LFFFFLSYVSTQNTSLLFWGEGPYV TNRKPLKLD*CGENTFSFWF*ETSSWVPGRGIP* L*YTWPPWYKSLVVWKNKFVFYVLFSVSTFQNG LKSLKPRHLLGPDPQ*LVTSVSGNLDFALKRAV VPVSRLWIFR*ILSFSFHFLKARVGS*KAMKQHA KCEMSTLTKLCRFRASDEDFIGFYSGFLIFGSP LKKGV*KLLYTVNDCLPMSCPKYHDSLWKLSLI KLDTVWLG

Frame 1

PKR*NPKVNLLVGDFDDSHKASRVMLMLQVLEP HFENHCYNVQQNCMISCMVALKTNRPQSQ*KPQ VGSHWRIQNEFGSPSMPHSQRTVII*PAGSSLKD VQGCFYLTGHLKLALYEQPPSSHACQKSEATNT AAS*ESHSVTQAGEQLHKHLAHCSLNFLSSSDP PTLAS*VAGTTVRSKNV*KPLR*KDQPL*TP*RQ KELCEL*EEKEDQHG*SEPWGGVL*KKMSLEVL VAKICIYFSGYKKE*KCTKQSSVKKKRR*PLDR PKPVLHCPVPMSHHCRCHPAREKG*RGC*RR*NQ G*VGTAEKIHKVVC*TCSSKATAEAQKGLCKEE REGTYREEGKS*CWQGGE*PCRKWRCQNRLGTE S*RCWRCQVKCVHF**LCTSGDCTV*NTIF*SSF IKMQILFSFFFFF*AMLVHRILHCCFGEKGHMS LIESP*SWTDVGKTPFPSGFERLPLGSQEEGFP DFDTHGHLGIKALWYGKTNSFFMSSSLFLPFRMD LNPLNPDIFWDLTPNNWLPVCQAIWTLPSKEQW FLFLGCGSSDKFCHFPFTS*KPGSVHEKL*NSM LNVKCQPSLNFAGSEHQMKTSLGFTVAF*FLVVH *RREFESCCILLTIVCPCPAQNTMILYGNYL** SWIQFGL

Frame 2

LSDEIQR*IFWLVILMIATKQAG*C*CFKSWNH TSRITVIMYSRTA*SAVW*P*KPTGHNHSENLR LAATGGYKMSLDLLQCPIPRELSLFDLLVVP*R TCKDVFI*LDT*SLPCMNSPHLPMLVKNQRQLTL RLPESLTLSPRLESNCTNTWLTAASTSCPQVIL PP*PPK*LGPQ*GQKMFESH*DRRTSHFERLRG KKSFVSCKRKKKTSMAEASPGEECYERR*VWKC* LQRYAFTSLATKKNRNAQSRAV*RRRGDDP*TD QSPCSTAQCLCPTTVAATLPERKAEGDAKGDKT KVK*ELQRRSTRLSAKPAPPKPQPKPRKASAKKR EKVPTEKKGKADAGKEGNNPAENGDAKTD*AQK AKGAGDAK*SVCIFDNCVLLVTVQFEILFFNQV L*KCRFCLAFFFFFKLC*YTEYFIVVLGRRAICH **KAPEAGLMWGKHLFLLVLRDFLLGPRKRDSL TLIHMATLV*KPCGMEKQIRFLCPLLCFYLSEW T*IP*TQTSSGT*PPIIGYQCVRQSGLCPQKSSG SCF*VVDLQINSVIFLSLPESQGRFMKSYETAC *M*NVNPH*TLPVQSIR*RLHWVLQWLSDFW*S IEEGSLKVVVYC*RLSAHVLPKIP*FFMEIIFNK AGYSLAW