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Sequences

Sequence NM_139273

ACTTCCGGGGCGGCGGTTGCATCAGATTCTAGGAAGTGTCTGTAGCCGCAGCTGCGGGTCCGGGATTCCCAGCCATGGCAGATTCCTCCGGGCAGCAGGG CAAAGGCCGGCGTGTGCAGCCCCAGTGGTCCCCTCCTGCTGGGACCCAGCCATGCAGACTCCACCTTTACAACAGCCTCACCAGGAACAAGGAAGTGTTC ATACCTCAAGATGGGAAAAAGGTGACGTGGTATTGCTGTGGGCCAACCGTCTATGACGCATCTCACATGGGGCACGCCAGGTCCTACATCTCTTTTGATA TCTTGAGAAGAGTGTTGAAGGATTACTTCAAATTTGATGTCTTTTATTGCATGAACATTACGGATATTGATGACAAGATCATCAAGAGGGCCCGGCAGAA CCACCTGTTCGAGCAGTATCGGGAGAAGAGGCCTGAAGCGGCACAGCTCTTGGAGGATGTTCAGGCCGCCCTGAAGCCATTTTCAGTAAAATTAAATGAG ACCACGGATCCCGATAAAAAGCAGATGCTCGAACGGATTCAGCACGCAGTGCAGCTTGCCACAGAGCCACTTGAGAAAGCTGTGCAGTCCAGACTCACGG GAGAGGAAGTCAACAGCTGTGTGGAGGTGTTGCTGGAAGAAGCCAAGGATTTGCTCTCTGACTGGCTGGATTCTACACTTGGCTGTGATGTCACTGACAA TTCCATCTTCTCCAAGCTGCCCAAGTTCTGGGAGGGGGACTTCCACAGAGACATGGAAGCTCTGAATGTTCTCCCTCCAGATGTCTTAACCCGGGTTAGT GAGTATGTGCCAGAAATTGTGAACTTTGTCCAGAAGATTGTGGACAACGGTTACGGCTATGTCTCCAATGGGTCTGTCTACTTTGATACAGCGAAGTTTG CTTCTAGCGAGAAGCACTCCTATGGGAAGCTGGTGCCTGAGGCCGTTGGAGATCAGAAAGCCCTTCAAGAAGGGGAAGGTGACCTGAGCATCTCTGCAGA CCGCCTGAGTGAGAAGCGCTCTCCCAACGACTTTGCCTTATGGAAGGCCTCTAAGCCCGGAGAACCGTCCTGGCCGTGCCCTTGGGGAAAGGGTCGTCCG GGCTGGCATATCGAGTGCTCGGCCATGGCAGGCACCCTCCTAGGGGCTTCGATGGACATTCACGGAGGTGGGTTCGACCTCCGGTTCCCCCACCATGACA ATGAGCTGGCACAGTCGGAGGCCTACTTTGAAAACGACTGCTGGGTCAGGTACTTCCTGCACACAGGCCACCTGACCATTGCAGGCTGCAAAATGTCAAA GTCACTAAAAAACTTCATCACCATTAAAGATGCCTTGAAAAAGCACTCAGCACGGCAGTTGCGGCTGGCCTTCCTCATGCACTCGTGGAAGGACACCCTG GACTACTCCAGCAACACCATGGAGTCAGCGCTTCAATATGAGAAGTTCTTGAATGAGTTTTTCTTAAATGTGAAAGATATCCTTCGCGCTCCTGTTGACA TCACTGGTCAGTTTGAGAAGTGGGGAGAAGAAGAAGCAGAACTGAATAAGAACTTTTATGACAAGAAGACAGCAATTCACAAAGCCCTCTGTGACAATGT TGACACCCGCACCGTCATGGAAGAGATGCGGGCCTTGGTCAGTCAGTGCAACCTCTATATGGCAGCCCGGAAAGCCGTGAGGAAGAGGCCCAACCAGGCT CTGCTGGAGAACATCGCCCTGTACCTCACCCATATGCTGAAGATCTTTGGGGCCGTAGAAGAGGACAGCTCCCTGGGATTCCCGGTCGGAGGGCCTGGAA CCAGCCTCAGTCTCGAGGCCACAGTCATGCCCTACCTTCAGGTGTTATCAGAATTCCGAGAAGGAGTGCGGAAGATTGCCCGAGAGCAAAAAGTCCCTGA GATTCTGCAGCTCAGCGATGCCCTGCGGGACAACATCCTGCCCGAGCTTGGGGTGCGGTTTGAAGACCACGAAGGACTGCCCACAGTGGTGAAACTGGTA GACAGAAACACCTTATTAAAAGAGAGAGAAGAAAAGAGACGGGTTGAAGAGGAGAAGAGGAAGAAGAAAGAGGAGGCGGCCCGGAGGAAACAGGAACAAG AAGCAGCAAAGCTGGCCAAGATGAAGATTCCCCCCAGTGAGATGTTCTTGTCAGAAACCGACAAATACTCCAAGTTTGATGAAAATGTAAGCATGGTCTG CCCACACATGACATGGAGGGCAAAGAGCTCAGCAAAGGGCAAGCCAAGAAGCTGAAGAAGCTCTTCGAGGCTCAGGAGAAGCTCTACAAGGAATATCTGC AGATGGCCCAGAATGGAAGCTTCCAGTGAGGGGGCACAGGACTGACTTTTTAAACCATTGTGGACTAGTGGCTGCTGTCTGCCTCAGTGACAATGTCCCA GCGCTCCTATCATGTTTACAGTCACCCTTGGGTCCTAAATTAAGAGTTGTGTTCATGTAGGTTCGTGTCGTCGTTGGCTCTGAGACATTGATAATAAATT TTTCTCAACAGTGA

refseq_CARS.F3 (forward primer)
refseq_CARS.R3 (reverse primer)
Single exon skipping

Frame 0

TSGAAVASDSRKCL*PQLRVRDSQPWQIPPGSR AKAGVCSPSGPLLLGPSHADSTFTTASPGTRKCS YLKMGKR*RGIAVGQPSMTHLTWGTPGPTSLLI S*EEC*RITSNLMSFIA*TLRILMTRSSRGPGR TTCSSSIGRRGLKRHSSWRMFRPP*SHFQ*N*MR PRIPIKSRCSNGFSTQCSLPQSHLRKLCSPDSR ERKSTAVWRCCWKKPRICSLTGWILHLAVMSLT IPSSPSCPSSGRGTSTETWKL*MFSLQMS*PGLV SMCQKL*TLSRRLWTTVTAMSPMGLSTLIQRSL LLARSTPMGSWCLRPLEIRKPFKKGKVT*ASLQ TA*VRSALPTTLPYGRPLSPENRPGRALGERVVR AGISSARPWQAPS*GLRWTFTEVGSTSGSPTMT MSWHSRRPTLKTTAGSGTSCTQAT*PLQAAKCQ SH*KTSSPLKMP*KSTQHGSCGWPSSCTRGRTPW TTPATPWSQRFNMRSS*MSFS*M*KISFALLLT SLVSLRSGEKKKQN*IRTFMTRRQQFTKPSVTM LTPAPSWKRCGPWSVSATSIWQPGKP*GRGPTRL CWRTSPCTSPIC*RSLGP*KRTAPWDSRSEGLE PASVSRPQSCPTFRCYQNSEKECGRLPESKKSL RFCSSAMPCGTTSCPSLGCGLKTTKDCPQW*NW* TETPY*KREKKRDGLKRRRGRRKRRRPGGNRNK KQQSWPR*RFPPVRCSCQKPTNTPSLMKM*AWS AHT*HGGQRAQQRASQEAEEALRGSGEALQGISA DGPEWKLPVRGHRTDFLNHCGLVAAVCLSDNVP ALLSCLQSPLGPKLRVVFM*VRVVVGSETLIIN FSQQ*

Frame 1

LPGRRLHQILGSVCSRSCGSGIPSHGRFLRAAG QRPACAAPVVPSCWDPAMQTPPLQQPHQEQGSV HTSRWEKGDVVLLWANRL*RISHGARQVLHLF*Y LEKSVEGLLQI*CLLLHEHYGY**QDHQEGPAE PPVRAVSGEEA*SGTALGGCSGRPEAIFSKIK* DHGSR*KADARTDSARSAACHRAT*ESCAVQTHG RGSQQLCGGVAGRSQGFAL*LAGFYTWL*CH*Q FHLLQAAQVLGGGLPQRHGSSECSPSRCLNPG* *VCARNCELCPEDCGQRLRLCLQWVCLL*YSEVC F*REALLWEAGA*GRWRSESPSRRGR*PEHLCR PPE*EALSQRLCLMEGL*ARRTVLAVPLGKGSS GLAYRVLGHGRHPPRGFDGHSRRWVRPPVPPP*Q *AGTVGGLL*KRLLGQVLPAHRPPDHCRLQNVK VTKKLHHH*RCLEKALSTAVAAGLPHALVEGHP GLLQQHHGVSASI*EVLE*VFLKCERYPSRSC*H HWSV*EVGRRRSRTE*ELL*QEDSNSQSPL*QC *HPHRHGRDAGLGQSVQPLYGSPESREEEAQPG SAGEHRPVPHPYAEDLWGRRRGQLPGIPGRRAWN QPQSRGHSHALPSGVIRIPRRSAEDCPRAKSP* DSAAQRCPAGQHPARAWGAV*RPRRTAHSGETG RQKHLIKRERRKETG*RGEEEEERGGGPEETGTR SSKAGQDEDSPQ*DVLVRNRQILQV**KCKHGL PTHDMEGKELSKGQAKKLKKLFEAQEKLYKEYL QMAQNGSFQ*GGTGLTF*TIVD*WLLSASVTMSQ RSYHVYSHPWVLN*ELCSCRFVSSLALRH***I FLNS

Frame 2

FRGGGCIRF*EVSVAAAAGPGFPAMADSSGQQG KGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVF IPQDGKKVTWYCCGPTVYDASHMGHARSYISFD ILRRVLKDYFKFDVFYCMNITDIDDKIIKRARQN HLFEQYREKRPEAAQLLEDVQAALKPFSVKLNE TTDPDKKQMLERIQHAVQLATEPLEKAVQSRLT GEEVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDN SIFSKLPKFWEGDFHRDMEALNVLPPDVLTRVS EYVPEIVNFVQKIVDNGYGYVSNGSVYFDTAKF ASSEKHSYGKLVPEAVGDQKALQEGEGDLSISAD RLSEKRSPNDFALWKASKPGEPSWPCPWGKGRP GWHIECSAMAGTLLGASMDIHGGGFDLRFPHHD NELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSK SLKNFITIKDALKKHSARQLRLAFLMHSWKDTL DYSSNTMESALQYEKFLNEFFLNVKDILRAPVD ITGQFEKWGEEEAELNKNFYDKKTAIHKALCDNV DTRTVMEEMRALVSQCNLYMAARKAVRKRPNQA LLENIALYLTHMLKIFGAVEEDSSLGFPVGGPG TSLSLEATVMPYLQVLSEFREGVRKIAREQKVPE ILQLSDALRDNILPELGVRFEDHEGLPTVVKLV DRNTLLKEREEKRRVEEEKRKKKEEAARRKQEQ EAAKLAKMKIPPSEMFLSETDKYSKFDENVSMVC PHMTWRAKSSAKGKPRS*RSSSRLRRSSTRNIC RWPRMEASSEGAQD*LFKPLWTSGCCLPQ*QCP SAPIMFTVTLGS*IKSCVHVGSCRRWL*DIDNKF FSTV

Sequence NM_001751

ACTTCCGGGGCGGCGGTTGCATCAGATTCTAGGAAGTGTCTGTAGCCGCAGCTGCGGGTCCGGGATTCCCAGCCATGGCAGATTCCTCCGGGCAGCAGGG CAAAGGCCGGCGTGTGCAGCCCCAGTGGTCCCCTCCTGCTGGGACCCAGCCATGCAGACTCCACCTTTACAACAGCCTCACCAGGAACAAGGAAGTGTTC ATACCTCAAGATGGGAAAAAGGTGACGTGGTATTGCTGTGGGCCAACCGTCTATGACGCATCTCACATGGGGCACGCCAGGTCCTACATCTCTTTTGATA TCTTGAGAAGAGTGTTGAAGGATTACTTCAAATTTGATGTCTTTTATTGCATGAACATTACGGATATTGATGACAAGATCATCAAGAGGGCCCGGCAGAA CCACCTGTTCGAGCAGTATCGGGAGAAGAGGCCTGAAGCGGCACAGCTCTTGGAGGATGTTCAGGCCGCCCTGAAGCCATTTTCAGTAAAATTAAATGAG ACCACGGATCCCGATAAAAAGCAGATGCTCGAACGGATTCAGCACGCAGTGCAGCTTGCCACAGAGCCACTTGAGAAAGCTGTGCAGTCCAGACTCACGG GAGAGGAAGTCAACAGCTGTGTGGAGGTGTTGCTGGAAGAAGCCAAGGATTTGCTCTCTGACTGGCTGGATTCTACACTTGGCTGTGATGTCACTGACAA TTCCATCTTCTCCAAGCTGCCCAAGTTCTGGGAGGGGGACTTCCACAGAGACATGGAAGCTCTGAATGTTCTCCCTCCAGATGTCTTAACCCGGGTTAGT GAGTATGTGCCAGAAATTGTGAACTTTGTCCAGAAGATTGTGGACAACGGTTACGGCTATGTCTCCAATGGGTCTGTCTACTTTGATACAGCGAAGTTTG CTTCTAGCGAGAAGCACTCCTATGGGAAGCTGGTGCCTGAGGCCGTTGGAGATCAGAAAGCCCTTCAAGAAGGGGAAGGTGACCTGAGCATCTCTGCAGA CCGCCTGAGTGAGAAGCGCTCTCCCAACGACTTTGCCTTATGGAAGGCCTCTAAGCCCGGAGAACCGTCCTGGCCGTGCCCTTGGGGAAAGGGTCGTCCG GGCTGGCATATCGAGTGCTCGGCCATGGCAGGCACCCTCCTAGGGGCTTCGATGGACATTCACGGAGGTGGGTTCGACCTCCGGTTCCCCCACCATGACA ATGAGCTGGCACAGTCGGAGGCCTACTTTGAAAACGACTGCTGGGTCAGGTACTTCCTGCACACAGGCCACCTGACCATTGCAGGCTGCAAAATGTCAAA GTCACTAAAAAACTTCATCACCATTAAAGATGCCTTGAAAAAGCACTCAGCACGGCAGTTGCGGCTGGCCTTCCTCATGCACTCGTGGAAGGACACCCTG GACTACTCCAGCAACACCATGGAGTCAGCGCTTCAATATGAGAAGTTCTTGAATGAGTTTTTCTTAAATGTGAAAGATATCCTTCGCGCTCCTGTTGACA TCACTGGTCAGTTTGAGAAGTGGGGAGAAGAAGAAGCAGAACTGAATAAGAACTTTTATGACAAGAAGACAGCAATTCACAAAGCCCTCTGTGACAATGT TGACACCCGCACCGTCATGGAAGAGATGCGGGCCTTGGTCAGTCAGTGCAACCTCTATATGGCAGCCCGGAAAGCCGTGAGGAAGAGGCCCAACCAGGCT CTGCTGGAGAACATCGCCCTGTACCTCACCCATATGCTGAAGATCTTTGGGGCCGTAGAAGAGGACAGCTCCCTGGGATTCCCGGTCGGAGGGCCTGGAA CCAGCCTCAGTCTCGAGGCCACAGTCATGCCCTACCTTCAGGTGTTATCAGAATTCCGAGAAGGAGTGCGGAAGATTGCCCGAGAGCAAAAAGTCCCTGA GATTCTGCAGCTCAGCGATGCCCTGCGGGACAACATCCTGCCCGAGCTTGGGGTGCGGTTTGAAGACCACGAAGGACTGCCCACAGTGGTGAAACTGGTA GACAGAAACACCTTATTAAAAGAGAGAGAAGAAAAGAGACGGGTTGAAGAGGAGAAGAGGAAGAAGAAAGAGGAGGCGGCCCGGAGGAAACAGGAACAAG AAGCAGCAAAGCTGGCCAAGATGAAGATTCCCCCCAGTGAGATGTTCTTGTCAGAAACCGACAAATACTCCAAGTTTGATGAAAATGGTCTGCCCACACA TGACATGGAGGGCAAAGAGCTCAGCAAAGGGCAAGCCAAGAAGCTGAAGAAGCTCTTCGAGGCTCAGGAGAAGCTCTACAAGGAATATCTGCAGATGGCC CAGAATGGAAGCTTCCAGTGAGGGGGCACAGGACTGACTTTTTAAACCATTGTGGACTAGTGGCTGCTGTCTGCCTCAGTGACAATGTCCCAGCGCTCCT ATCATGTTTACAGTCACCCTTGGGTCCTAAATTAAGAGTTGTGTTCATGTAGGTTCGTGTCGTCGTTGGCTCTGAGACATTGATAATAAATTTTTCTCAA CAGTGA

refseq_CARS.F3 (forward primer)
refseq_CARS.R3 (reverse primer)
Single exon skipping

Frame 0

TSGAAVASDSRKCL*PQLRVRDSQPWQIPPGSR AKAGVCSPSGPLLLGPSHADSTFTTASPGTRKCS YLKMGKR*RGIAVGQPSMTHLTWGTPGPTSLLI S*EEC*RITSNLMSFIA*TLRILMTRSSRGPGR TTCSSSIGRRGLKRHSSWRMFRPP*SHFQ*N*MR PRIPIKSRCSNGFSTQCSLPQSHLRKLCSPDSR ERKSTAVWRCCWKKPRICSLTGWILHLAVMSLT IPSSPSCPSSGRGTSTETWKL*MFSLQMS*PGLV SMCQKL*TLSRRLWTTVTAMSPMGLSTLIQRSL LLARSTPMGSWCLRPLEIRKPFKKGKVT*ASLQ TA*VRSALPTTLPYGRPLSPENRPGRALGERVVR AGISSARPWQAPS*GLRWTFTEVGSTSGSPTMT MSWHSRRPTLKTTAGSGTSCTQAT*PLQAAKCQ SH*KTSSPLKMP*KSTQHGSCGWPSSCTRGRTPW TTPATPWSQRFNMRSS*MSFS*M*KISFALLLT SLVSLRSGEKKKQN*IRTFMTRRQQFTKPSVTM LTPAPSWKRCGPWSVSATSIWQPGKP*GRGPTRL CWRTSPCTSPIC*RSLGP*KRTAPWDSRSEGLE PASVSRPQSCPTFRCYQNSEKECGRLPESKKSL RFCSSAMPCGTTSCPSLGCGLKTTKDCPQW*NW* TETPY*KREKKRDGLKRRRGRRKRRRPGGNRNK KQQSWPR*RFPPVRCSCQKPTNTPSLMKMVCPH MTWRAKSSAKGKPRS*RSSSRLRRSSTRNICRWP RMEASSEGAQD*LFKPLWTSGCCLPQ*QCPSAP IMFTVTLGS*IKSCVHVGSCRRWL*DIDNKFFS TV

Frame 1

LPGRRLHQILGSVCSRSCGSGIPSHGRFLRAAG QRPACAAPVVPSCWDPAMQTPPLQQPHQEQGSV HTSRWEKGDVVLLWANRL*RISHGARQVLHLF*Y LEKSVEGLLQI*CLLLHEHYGY**QDHQEGPAE PPVRAVSGEEA*SGTALGGCSGRPEAIFSKIK* DHGSR*KADARTDSARSAACHRAT*ESCAVQTHG RGSQQLCGGVAGRSQGFAL*LAGFYTWL*CH*Q FHLLQAAQVLGGGLPQRHGSSECSPSRCLNPG* *VCARNCELCPEDCGQRLRLCLQWVCLL*YSEVC F*REALLWEAGA*GRWRSESPSRRGR*PEHLCR PPE*EALSQRLCLMEGL*ARRTVLAVPLGKGSS GLAYRVLGHGRHPPRGFDGHSRRWVRPPVPPP*Q *AGTVGGLL*KRLLGQVLPAHRPPDHCRLQNVK VTKKLHHH*RCLEKALSTAVAAGLPHALVEGHP GLLQQHHGVSASI*EVLE*VFLKCERYPSRSC*H HWSV*EVGRRRSRTE*ELL*QEDSNSQSPL*QC *HPHRHGRDAGLGQSVQPLYGSPESREEEAQPG SAGEHRPVPHPYAEDLWGRRRGQLPGIPGRRAWN QPQSRGHSHALPSGVIRIPRRSAEDCPRAKSP* DSAAQRCPAGQHPARAWGAV*RPRRTAHSGETG RQKHLIKRERRKETG*RGEEEEERGGGPEETGTR SSKAGQDEDSPQ*DVLVRNRQILQV**KWSAHT *HGGQRAQQRASQEAEEALRGSGEALQGISADG PEWKLPVRGHRTDFLNHCGLVAAVCLSDNVPALL SCLQSPLGPKLRVVFM*VRVVVGSETLIINFSQ Q*

Frame 2

FRGGGCIRF*EVSVAAAAGPGFPAMADSSGQQG KGRRVQPQWSPPAGTQPCRLHLYNSLTRNKEVF IPQDGKKVTWYCCGPTVYDASHMGHARSYISFD ILRRVLKDYFKFDVFYCMNITDIDDKIIKRARQN HLFEQYREKRPEAAQLLEDVQAALKPFSVKLNE TTDPDKKQMLERIQHAVQLATEPLEKAVQSRLT GEEVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDN SIFSKLPKFWEGDFHRDMEALNVLPPDVLTRVS EYVPEIVNFVQKIVDNGYGYVSNGSVYFDTAKF ASSEKHSYGKLVPEAVGDQKALQEGEGDLSISAD RLSEKRSPNDFALWKASKPGEPSWPCPWGKGRP GWHIECSAMAGTLLGASMDIHGGGFDLRFPHHD NELAQSEAYFENDCWVRYFLHTGHLTIAGCKMSK SLKNFITIKDALKKHSARQLRLAFLMHSWKDTL DYSSNTMESALQYEKFLNEFFLNVKDILRAPVD ITGQFEKWGEEEAELNKNFYDKKTAIHKALCDNV DTRTVMEEMRALVSQCNLYMAARKAVRKRPNQA LLENIALYLTHMLKIFGAVEEDSSLGFPVGGPG TSLSLEATVMPYLQVLSEFREGVRKIAREQKVPE ILQLSDALRDNILPELGVRFEDHEGLPTVVKLV DRNTLLKEREEKRRVEEEKRKKKEEAARRKQEQ EAAKLAKMKIPPSEMFLSETDKYSKFDENGLPTH DMEGKELSKGQAKKLKKLFEAQEKLYKEYLQMA QNGSFQ*GGTGLTF*TIVD*WLLSASVTMSQRS YHVYSHPWVLN*ELCSCRFVSSLALRH***IFLN S

Sequence NM_001014437

ACTTCCGGGGCGGCGGTTGCATCAGATTCTAGGAAGTGTCTGTAGCCGCAGCTGCGGGTCCGGGATTCCCAGCCATGGCAGATTCCTCCGGGCAGCAGGC TCCTGACTACAGGTCCATTCTGAGCATTAGTGACGAGGCAGCCAGGGCACAAGCCCTGAACGAGCACCTCAGCACGCGTAGCTATGTCCAGGGGTACTCA CTGTCCCAGGCAGACGTGGACGCGTTCAGGCAGCTCTCGGCCCCGCCCGCTGACCCCCAGCTCTTCCACGTGGCTCGGTGGTTCAGGCACATAGAAGCGC TCCTGGGTAGCCCCTGTGGCAAAGGCCAGCCCTGCAGGCTCCAAGCAAGCAAAGGCCGGCGTGTGCAGCCCCAGTGGTCCCCTCCTGCTGGGACCCAGCC ATGCAGACTCCACCTTTACAACAGCCTCACCAGGAACAAGGAAGTGTTCATACCTCAAGATGGGAAAAAGGTGACGTGGTATTGCTGTGGGCCAACCGTC TATGACGCATCTCACATGGGGCACGCCAGGTCCTACATCTCTTTTGATATCTTGAGAAGAGTGTTGAAGGATTACTTCAAATTTGATGTCTTTTATTGCA TGAACATTACGGATATTGATGACAAGATCATCAAGAGGGCCCGGCAGAACCACCTGTTCGAGCAGTATCGGGAGAAGAGGCCTGAAGCGGCACAGCTCTT GGAGGATGTTCAGGCCGCCCTGAAGCCATTTTCAGTAAAATTAAATGAGACCACGGATCCCGATAAAAAGCAGATGCTCGAACGGATTCAGCACGCAGTG CAGCTTGCCACAGAGCCACTTGAGAAAGCTGTGCAGTCCAGACTCACGGGAGAGGAAGTCAACAGCTGTGTGGAGGTGTTGCTGGAAGAAGCCAAGGATT TGCTCTCTGACTGGCTGGATTCTACACTTGGCTGTGATGTCACTGACAATTCCATCTTCTCCAAGCTGCCCAAGTTCTGGGAGGGGGACTTCCACAGAGA CATGGAAGCTCTGAATGTTCTCCCTCCAGATGTCTTAACCCGGGTTAGTGAGTATGTGCCAGAAATTGTGAACTTTGTCCAGAAGATTGTGGACAACGGT TACGGCTATGTCTCCAATGGGTCTGTCTACTTTGATACAGCGAAGTTTGCTTCTAGCGAGAAGCACTCCTATGGGAAGCTGGTGCCTGAGGCCGTTGGAG ATCAGAAAGCCCTTCAAGAAGGGGAAGGTGACCTGAGCATCTCTGCAGACCGCCTGAGTGAGAAGCGCTCTCCCAACGACTTTGCCTTATGGAAGGCCTC TAAGCCCGGAGAACCGTCCTGGCCGTGCCCTTGGGGAAAGGGTCGTCCGGGCTGGCATATCGAGTGCTCGGCCATGGCAGGCACCCTCCTAGGGGCTTCG ATGGACATTCACGGAGGTGGGTTCGACCTCCGGTTCCCCCACCATGACAATGAGCTGGCACAGTCGGAGGCCTACTTTGAAAACGACTGCTGGGTCAGGT ACTTCCTGCACACAGGCCACCTGACCATTGCAGGCTGCAAAATGTCAAAGTCACTAAAAAACTTCATCACCATTAAAGATGCCTTGAAAAAGCACTCAGC ACGGCAGTTGCGGCTGGCCTTCCTCATGCACTCGTGGAAGGACACCCTGGACTACTCCAGCAACACCATGGAGTCAGCGCTTCAATATGAGAAGTTCTTG AATGAGTTTTTCTTAAATGTGAAAGATATCCTTCGCGCTCCTGTTGACATCACTGGTCAGTTTGAGAAGTGGGGAGAAGAAGAAGCAGAACTGAATAAGA ACTTTTATGACAAGAAGACAGCAATTCACAAAGCCCTCTGTGACAATGTTGACACCCGCACCGTCATGGAAGAGATGCGGGCCTTGGTCAGTCAGTGCAA CCTCTATATGGCAGCCCGGAAAGCCGTGAGGAAGAGGCCCAACCAGGCTCTGCTGGAGAACATCGCCCTGTACCTCACCCATATGCTGAAGATCTTTGGG GCCGTAGAAGAGGACAGCTCCCTGGGATTCCCGGTCGGAGGGCCTGGAACCAGCCTCAGTCTCGAGGCCACAGTCATGCCCTACCTTCAGGTGTTATCAG AATTCCGAGAAGGAGTGCGGAAGATTGCCCGAGAGCAAAAAGTCCCTGAGATTCTGCAGCTCAGCGATGCCCTGCGGGACAACATCCTGCCCGAGCTTGG GGTGCGGTTTGAAGACCACGAAGGACTGCCCACAGTGGTGAAACTGGTAGACAGAAACACCTTATTAAAAGAGAGAGAAGAAAAGAGACGGGTTGAAGAG GAGAAGAGGAAGAAGAAAGAGGAGGCGGCCCGGAGGAAACAGGAACAAGAAGCAGCAAAGCTGGCCAAGATGAAGATTCCCCCCAGTGAGATGTTCTTGT CAGAAACCGACAAATACTCCAAGTTTGATGAAAATGGTCTGCCCACACATGACATGGAGGGCAAAGAGCTCAGCAAAGGGCAAGCCAAGAAGCTGAAGAA GCTCTTCGAGGCTCAGGAGAAGCTCTACAAGGAATATCTGCAGATGGCCCAGAATGGAAGCTTCCAGTGAGGGGGCACAGGACTGACTTTTTAAACCATT GTGGACTAGTGGCTGCTGTCTGCCTCAGTGACAATGTCCCAGCGCTCCTATCATGTTTACAGTCACCCTTGGGTCCTAAATTAAGAGTTGTGTTCATGTA GGTTCGTGTCGTCGTTGGCTCTGAGACATTGATAATAAATTTTTCTCAACAGTGA

refseq_CARS.F3 (forward primer)
refseq_CARS.R3 (reverse primer)
Single exon skipping

Frame 0

TSGAAVASDSRKCL*PQLRVRDSQPWQIPPGSR LLTTGPF*ALVTRQPGHKP*TSTSARVAMSRGTH CPRQTWTRSGSSRPRPLTPSSSTWLGGSGT*KR SWVAPVAKASPAGSKQAKAGVCSPSGPLLLGPS HADSTFTTASPGTRKCSYLKMGKR*RGIAVGQPS MTHLTWGTPGPTSLLIS*EEC*RITSNLMSFIA *TLRILMTRSSRGPGRTTCSSSIGRRGLKRHSS WRMFRPP*SHFQ*N*MRPRIPIKSRCSNGFSTQC SLPQSHLRKLCSPDSRERKSTAVWRCCWKKPRI CSLTGWILHLAVMSLTIPSSPSCPSSGRGTSTE TWKL*MFSLQMS*PGLVSMCQKL*TLSRRLWTTV TAMSPMGLSTLIQRSLLLARSTPMGSWCLRPLE IRKPFKKGKVT*ASLQTA*VRSALPTTLPYGRP LSPENRPGRALGERVVRAGISSARPWQAPS*GLR WTFTEVGSTSGSPTMTMSWHSRRPTLKTTAGSG TSCTQAT*PLQAAKCQSH*KTSSPLKMP*KSTQ HGSCGWPSSCTRGRTPWTTPATPWSQRFNMRSS* MSFS*M*KISFALLLTSLVSLRSGEKKKQN*IR TFMTRRQQFTKPSVTMLTPAPSWKRCGPWSVSA TSIWQPGKP*GRGPTRLCWRTSPCTSPIC*RSLG P*KRTAPWDSRSEGLEPASVSRPQSCPTFRCYQ NSEKECGRLPESKKSLRFCSSAMPCGTTSCPSL GCGLKTTKDCPQW*NW*TETPY*KREKKRDGLKR RRGRRKRRRPGGNRNKKQQSWPR*RFPPVRCSC QKPTNTPSLMKMVCPHMTWRAKSSAKGKPRS*R SSSRLRRSSTRNICRWPRMEASSEGAQD*LFKPL WTSGCCLPQ*QCPSAPIMFTVTLGS*IKSCVHV GSCRRWL*DIDNKFFSTV

Frame 1

LPGRRLHQILGSVCSRSCGSGIPSHGRFLRAAG S*LQVHSEH**RGSQGTSPERAPQHA*LCPGVL TVPGRRGRVQAALGPAR*PPALPRGSVVQAHRSA PG*PLWQRPALQAPSKQRPACAAPVVPSCWDPA MQTPPLQQPHQEQGSVHTSRWEKGDVVLLWANR L*RISHGARQVLHLF*YLEKSVEGLLQI*CLLLH EHYGY**QDHQEGPAEPPVRAVSGEEA*SGTAL GGCSGRPEAIFSKIK*DHGSR*KADARTDSARS AACHRAT*ESCAVQTHGRGSQQLCGGVAGRSQGF AL*LAGFYTWL*CH*QFHLLQAAQVLGGGLPQR HGSSECSPSRCLNPG**VCARNCELCPEDCGQR LRLCLQWVCLL*YSEVCF*REALLWEAGA*GRWR SESPSRRGR*PEHLCRPPE*EALSQRLCLMEGL *ARRTVLAVPLGKGSSGLAYRVLGHGRHPPRGF DGHSRRWVRPPVPPP*Q*AGTVGGLL*KRLLGQV LPAHRPPDHCRLQNVKVTKKLHHH*RCLEKALS TAVAAGLPHALVEGHPGLLQQHHGVSASI*EVL E*VFLKCERYPSRSC*HHWSV*EVGRRRSRTE*E LL*QEDSNSQSPL*QC*HPHRHGRDAGLGQSVQ PLYGSPESREEEAQPGSAGEHRPVPHPYAEDLW GRRRGQLPGIPGRRAWNQPQSRGHSHALPSGVIR IPRRSAEDCPRAKSP*DSAAQRCPAGQHPARAW GAV*RPRRTAHSGETGRQKHLIKRERRKETG*R GEEEEERGGGPEETGTRSSKAGQDEDSPQ*DVLV RNRQILQV**KWSAHT*HGGQRAQQRASQEAEE ALRGSGEALQGISADGPEWKLPVRGHRTDFLNH CGLVAAVCLSDNVPALLSCLQSPLGPKLRVVFM* VRVVVGSETLIINFSQQ*

Frame 2

FRGGGCIRF*EVSVAAAAGPGFPAMADSSGQQA PDYRSILSISDEAARAQALNEHLSTRSYVQGYS LSQADVDAFRQLSAPPADPQLFHVARWFRHIEA LLGSPCGKGQPCRLQASKGRRVQPQWSPPAGTQP CRLHLYNSLTRNKEVFIPQDGKKVTWYCCGPTV YDASHMGHARSYISFDILRRVLKDYFKFDVFYC MNITDIDDKIIKRARQNHLFEQYREKRPEAAQLL EDVQAALKPFSVKLNETTDPDKKQMLERIQHAV QLATEPLEKAVQSRLTGEEVNSCVEVLLEEAKD LLSDWLDSTLGCDVTDNSIFSKLPKFWEGDFHRD MEALNVLPPDVLTRVSEYVPEIVNFVQKIVDNG YGYVSNGSVYFDTAKFASSEKHSYGKLVPEAVG DQKALQEGEGDLSISADRLSEKRSPNDFALWKAS KPGEPSWPCPWGKGRPGWHIECSAMAGTLLGAS MDIHGGGFDLRFPHHDNELAQSEAYFENDCWVR YFLHTGHLTIAGCKMSKSLKNFITIKDALKKHSA RQLRLAFLMHSWKDTLDYSSNTMESALQYEKFL NEFFLNVKDILRAPVDITGQFEKWGEEEAELNK NFYDKKTAIHKALCDNVDTRTVMEEMRALVSQCN LYMAARKAVRKRPNQALLENIALYLTHMLKIFG AVEEDSSLGFPVGGPGTSLSLEATVMPYLQVLS EFREGVRKIAREQKVPEILQLSDALRDNILPELG VRFEDHEGLPTVVKLVDRNTLLKEREEKRRVEE EKRKKKEEAARRKQEQEAAKLAKMKIPPSEMFL SETDKYSKFDENGLPTHDMEGKELSKGQAKKLKK LFEAQEKLYKEYLQMAQNGSFQ*GGTGLTF*TI VD*WLLSASVTMSQRSYHVYSHPWVLN*ELCSC RFVSSLALRH***IFLNS

Sequence NM_001014438

ACTTCCGGGGCGGCGGTTGCATCAGATTCTAGGAAGTGTCTGTAGCCGCAGCTGCGGGTCCGGGATTCCCAGCCATGGCAGATTCCTCCGGGCAGCAGGC TCCTGACTACAGGTCCATTCTGAGCATTAGTGACGAGGCAGCCAGGGCACAAGCCCTGAACGAGCACCTCAGCACGCGTAGCTATGTCCAGGGGTACTCA CTGTCCCAGGCAGACGTGGACGCGTTCAGGCAGCTCTCGGCCCCGCCCGCTGACCCCCAGCTCTTCCACGTGGCTCGGTGGTTCAGGCACATAGAAGCGC TCCTGGGTAGCCCCTGTGGCAAAGGCCAGCCCTGCAGGCTCCAAGCAAGCAAAGGCCGGCGTGTGCAGCCCCAGTGGTCCCCTCCTGCTGGGACCCAGCC ATGCAGACTCCACCTTTACAACAGCCTCACCAGGAACAAGTGTTTCTTGCATTTCTTGTGATCGTCATTCCTTCATTCCTAACAAAGGAAGTGTTCATAC CTCAAGATGGGAAAAAGGTGACGTGGTATTGCTGTGGGCCAACCGTCTATGACGCATCTCACATGGGGCACGCCAGGTCCTACATCTCTTTTGATATCTT GAGAAGAGTGTTGAAGGATTACTTCAAATTTGATGTCTTTTATTGCATGAACATTACGGATATTGATGACAAGATCATCAAGAGGGCCCGGCAGAACCAC CTGTTCGAGCAGTATCGGGAGAAGAGGCCTGAAGCGGCACAGCTCTTGGAGGATGTTCAGGCCGCCCTGAAGCCATTTTCAGTAAAATTAAATGAGACCA CGGATCCCGATAAAAAGCAGATGCTCGAACGGATTCAGCACGCAGTGCAGCTTGCCACAGAGCCACTTGAGAAAGCTGTGCAGTCCAGACTCACGGGAGA GGAAGTCAACAGCTGTGTGGAGGTGTTGCTGGAAGAAGCCAAGGATTTGCTCTCTGACTGGCTGGATTCTACACTTGGCTGTGATGTCACTGACAATTCC ATCTTCTCCAAGCTGCCCAAGTTCTGGGAGGGGGACTTCCACAGAGACATGGAAGCTCTGAATGTTCTCCCTCCAGATGTCTTAACCCGGGTTAGTGAGT ATGTGCCAGAAATTGTGAACTTTGTCCAGAAGATTGTGGACAACGGTTACGGCTATGTCTCCAATGGGTCTGTCTACTTTGATACAGCGAAGTTTGCTTC TAGCGAGAAGCACTCCTATGGGAAGCTGGTGCCTGAGGCCGTTGGAGATCAGAAAGCCCTTCAAGAAGGGGAAGGTGACCTGAGCATCTCTGCAGACCGC CTGAGTGAGAAGCGCTCTCCCAACGACTTTGCCTTATGGAAGGCCTCTAAGCCCGGAGAACCGTCCTGGCCGTGCCCTTGGGGAAAGGGTCGTCCGGGCT GGCATATCGAGTGCTCGGCCATGGCAGGCACCCTCCTAGGGGCTTCGATGGACATTCACGGAGGTGGGTTCGACCTCCGGTTCCCCCACCATGACAATGA GCTGGCACAGTCGGAGGCCTACTTTGAAAACGACTGCTGGGTCAGGTACTTCCTGCACACAGGCCACCTGACCATTGCAGGCTGCAAAATGTCAAAGTCA CTAAAAAACTTCATCACCATTAAAGATGCCTTGAAAAAGCACTCAGCACGGCAGTTGCGGCTGGCCTTCCTCATGCACTCGTGGAAGGACACCCTGGACT ACTCCAGCAACACCATGGAGTCAGCGCTTCAATATGAGAAGTTCTTGAATGAGTTTTTCTTAAATGTGAAAGATATCCTTCGCGCTCCTGTTGACATCAC TGGTCAGTTTGAGAAGTGGGGAGAAGAAGAAGCAGAACTGAATAAGAACTTTTATGACAAGAAGACAGCAATTCACAAAGCCCTCTGTGACAATGTTGAC ACCCGCACCGTCATGGAAGAGATGCGGGCCTTGGTCAGTCAGTGCAACCTCTATATGGCAGCCCGGAAAGCCGTGAGGAAGAGGCCCAACCAGGCTCTGC TGGAGAACATCGCCCTGTACCTCACCCATATGCTGAAGATCTTTGGGGCCGTAGAAGAGGACAGCTCCCTGGGATTCCCGGTCGGAGGGCCTGGAACCAG CCTCAGTCTCGAGGCCACAGTCATGCCCTACCTTCAGGTGTTATCAGAATTCCGAGAAGGAGTGCGGAAGATTGCCCGAGAGCAAAAAGTCCCTGAGATT CTGCAGCTCAGCGATGCCCTGCGGGACAACATCCTGCCCGAGCTTGGGGTGCGGTTTGAAGACCACGAAGGACTGCCCACAGTGGTGAAACTGGTAGACA GAAACACCTTATTAAAAGAGAGAGAAGAAAAGAGACGGGTTGAAGAGGAGAAGAGGAAGAAGAAAGAGGAGGCGGCCCGGAGGAAACAGGAACAAGAAGC AGCAAAGCTGGCCAAGATGAAGATTCCCCCCAGTGAGATGTTCTTGTCAGAAACCGACAAATACTCCAAGTTTGATGAAAATGGTCTGCCCACACATGAC ATGGAGGGCAAAGAGCTCAGCAAAGGGCAAGCCAAGAAGCTGAAGAAGCTCTTCGAGGCTCAGGAGAAGCTCTACAAGGAATATCTGCAGATGGCCCAGA ATGGAAGCTTCCAGTGAGGGGGCACAGGACTGACTTTTTAAACCATTGTGGACTAGTGGCTGCTGTCTGCCTCAGTGACAATGTCCCAGCGCTCCTATCA TGTTTACAGTCACCCTTGGGTCCTAAATTAAGAGTTGTGTTCATGTAGGTTCGTGTCGTCGTTGGCTCTGAGACATTGATAATAAATTTTTCTCAACAGT GA

refseq_CARS.F3 (forward primer)
refseq_CARS.R3 (reverse primer)
Single exon skipping

Frame 0

TSGAAVASDSRKCL*PQLRVRDSQPWQIPPGSR LLTTGPF*ALVTRQPGHKP*TSTSARVAMSRGTH CPRQTWTRSGSSRPRPLTPSSSTWLGGSGT*KR SWVAPVAKASPAGSKQAKAGVCSPSGPLLLGPS HADSTFTTASPGTSVSCISCDRHSFIPNKGSVHT SRWEKGDVVLLWANRL*RISHGARQVLHLF*YL EKSVEGLLQI*CLLLHEHYGY**QDHQEGPAEP PVRAVSGEEA*SGTALGGCSGRPEAIFSKIK*DH GSR*KADARTDSARSAACHRAT*ESCAVQTHGR GSQQLCGGVAGRSQGFAL*LAGFYTWL*CH*QF HLLQAAQVLGGGLPQRHGSSECSPSRCLNPG**V CARNCELCPEDCGQRLRLCLQWVCLL*YSEVCF *REALLWEAGA*GRWRSESPSRRGR*PEHLCRP PE*EALSQRLCLMEGL*ARRTVLAVPLGKGSSGL AYRVLGHGRHPPRGFDGHSRRWVRPPVPPP*Q* AGTVGGLL*KRLLGQVLPAHRPPDHCRLQNVKV TKKLHHH*RCLEKALSTAVAAGLPHALVEGHPGL LQQHHGVSASI*EVLE*VFLKCERYPSRSC*HH WSV*EVGRRRSRTE*ELL*QEDSNSQSPL*QC* HPHRHGRDAGLGQSVQPLYGSPESREEEAQPGSA GEHRPVPHPYAEDLWGRRRGQLPGIPGRRAWNQ PQSRGHSHALPSGVIRIPRRSAEDCPRAKSP*D SAAQRCPAGQHPARAWGAV*RPRRTAHSGETGRQ KHLIKRERRKETG*RGEEEEERGGGPEETGTRS SKAGQDEDSPQ*DVLVRNRQILQV**KWSAHT* HGGQRAQQRASQEAEEALRGSGEALQGISADGPE WKLPVRGHRTDFLNHCGLVAAVCLSDNVPALLS CLQSPLGPKLRVVFM*VRVVVGSETLIINFSQQ *

Frame 1

LPGRRLHQILGSVCSRSCGSGIPSHGRFLRAAG S*LQVHSEH**RGSQGTSPERAPQHA*LCPGVL TVPGRRGRVQAALGPAR*PPALPRGSVVQAHRSA PG*PLWQRPALQAPSKQRPACAAPVVPSCWDPA MQTPPLQQPHQEQVFLAFLVIVIPSFLTKEVFI PQDGKKVTWYCCGPTVYDASHMGHARSYISFDIL RRVLKDYFKFDVFYCMNITDIDDKIIKRARQNH LFEQYREKRPEAAQLLEDVQAALKPFSVKLNET TDPDKKQMLERIQHAVQLATEPLEKAVQSRLTGE EVNSCVEVLLEEAKDLLSDWLDSTLGCDVTDNS IFSKLPKFWEGDFHRDMEALNVLPPDVLTRVSE YVPEIVNFVQKIVDNGYGYVSNGSVYFDTAKFAS SEKHSYGKLVPEAVGDQKALQEGEGDLSISADR LSEKRSPNDFALWKASKPGEPSWPCPWGKGRPG WHIECSAMAGTLLGASMDIHGGGFDLRFPHHDNE LAQSEAYFENDCWVRYFLHTGHLTIAGCKMSKS LKNFITIKDALKKHSARQLRLAFLMHSWKDTLD YSSNTMESALQYEKFLNEFFLNVKDILRAPVDIT GQFEKWGEEEAELNKNFYDKKTAIHKALCDNVD TRTVMEEMRALVSQCNLYMAARKAVRKRPNQAL LENIALYLTHMLKIFGAVEEDSSLGFPVGGPGTS LSLEATVMPYLQVLSEFREGVRKIAREQKVPEI LQLSDALRDNILPELGVRFEDHEGLPTVVKLVD RNTLLKEREEKRRVEEEKRKKKEEAARRKQEQEA AKLAKMKIPPSEMFLSETDKYSKFDENGLPTHD MEGKELSKGQAKKLKKLFEAQEKLYKEYLQMAQ NGSFQ*GGTGLTF*TIVD*WLLSASVTMSQRSYH VYSHPWVLN*ELCSCRFVSSLALRH***IFLNS

Frame 2

FRGGGCIRF*EVSVAAAAGPGFPAMADSSGQQA PDYRSILSISDEAARAQALNEHLSTRSYVQGYS LSQADVDAFRQLSAPPADPQLFHVARWFRHIEA LLGSPCGKGQPCRLQASKGRRVQPQWSPPAGTQP CRLHLYNSLTRNKCFLHFL*SSFLHS*QRKCSY LKMGKR*RGIAVGQPSMTHLTWGTPGPTSLLIS *EEC*RITSNLMSFIA*TLRILMTRSSRGPGRTT CSSSIGRRGLKRHSSWRMFRPP*SHFQ*N*MRP RIPIKSRCSNGFSTQCSLPQSHLRKLCSPDSRE RKSTAVWRCCWKKPRICSLTGWILHLAVMSLTIP SSPSCPSSGRGTSTETWKL*MFSLQMS*PGLVS MCQKL*TLSRRLWTTVTAMSPMGLSTLIQRSLL LARSTPMGSWCLRPLEIRKPFKKGKVT*ASLQTA *VRSALPTTLPYGRPLSPENRPGRALGERVVRA GISSARPWQAPS*GLRWTFTEVGSTSGSPTMTM SWHSRRPTLKTTAGSGTSCTQAT*PLQAAKCQSH *KTSSPLKMP*KSTQHGSCGWPSSCTRGRTPWT TPATPWSQRFNMRSS*MSFS*M*KISFALLLTS LVSLRSGEKKKQN*IRTFMTRRQQFTKPSVTMLT PAPSWKRCGPWSVSATSIWQPGKP*GRGPTRLC WRTSPCTSPIC*RSLGP*KRTAPWDSRSEGLEP ASVSRPQSCPTFRCYQNSEKECGRLPESKKSLRF CSSAMPCGTTSCPSLGCGLKTTKDCPQW*NW*T ETPY*KREKKRDGLKRRRGRRKRRRPGGNRNKK QQSWPR*RFPPVRCSCQKPTNTPSLMKMVCPHMT WRAKSSAKGKPRS*RSSSRLRRSSTRNICRWPR MEASSEGAQD*LFKPLWTSGCCLPQ*QCPSAPI MFTVTLGS*IKSCVHVGSCRRWL*DIDNKFFSTV