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Sequence NM_181640
ATGCGCGCAAGAGAGCGGGAAGCCGAGCTGGGCGAGAAGTAGGGGAGGGCGGTGCTCCGCCGCGGTGGCGGTTGCTATCGCTTCGCAGAACCTACTCAGGCAGCCAGCTGAGAAGAGTTGAGGGAAAGTGCTGCTGCTGGGTCTGCAGACGCGATGGATAACGTGCAGCCGAAAATAAAACATCGCCCCTTCTGCTTCAG
TGTGAAAGGCCACGTGAAGATGCTGCGGCTGGATATTATCAACTCACTGGTAACAACAGTATTCATGCTCATCGTATCTGTGTTGGCACTGATACCAGAA
ACCACAACATTGACAGTTGGTGGAGGGGTGTTTGCACTTGTGACAGCAGTATGCTGTCTTGCCGACGGGGCCCTTATTTACCGGAAGCTTCTGTTCAATC
CCAGCGGTCCTTACCAGAAAAAGCCTGTGCATGAAAAAAAAGAAGTTTTGTAATTTTATATTACTTTTTAGTTTGATACTAAGTATTAAACATATTTCTG
TATTCTTCCA
- refseq_CKLF.F3 (forward primer)
- refseq_CKLF.R3 (reverse primer)
- Single exon skipping
Frame 0
MRAREREAELGEK*GRAVLRRGGGCYRFAEPTQAAS*EELRESAAAGSADAMDNVQPKIKHRPFCFS
VKGHVKMLRLDIINSLVTTVFMLIVSVLALIPE
TTTLTVGGGVFALVTAVCCLADGALIYRKLLFN
PSGPYQKKPVHEKKEVL*FYITF*FDTKY*TYFC
ILP
Frame 1
CAQESGKPSWARSRGGRCSAAVAVAIASQNLLRQPAEKS*GKVLLLGLQTRWITCSRK*NIAPSAS
V*KAT*RCCGWILSTHW*QQYSCSSYLCWH*YQK
PQH*QLVEGCLHL*QQYAVLPTGPLFTGSFCSI
PAVLTRKSLCMKKKKFCNFILLFSLILSIKHIS
VFF
Frame 2
ARKRAGSRAGREVGEGGAPPRWRLLSLRRTYSGSQLRRVEGKCCCWVCRRDG*RAAENKTSPLLLQ
CERPREDAAAGYYQLTGNNSIHAHRICVGTDTR
NHNIDSWWRGVCTCDSSMLSCRRGPYLPEASVQS
QRSLPEKACA*KKRSFVILYYFLV*Y*VLNIFL
YSS
Sequence NM_016951
ATGCGCGCAAGAGAGCGGGAAGCCGAGCTGGGCGAGAAGTAGGGGAGGGCGGTGCTCCGCCGCGGTGGCGGTTGCTATCGCTTCGCAGAACCTACTCAGGCAGCCAGCTGAGAAGAGTTGAGGGAAAGTGCTGCTGCTGGGTCTGCAGACGCGATGGATAACGTGCAGCCGAAAATAAAACATCGCCCCTTCTGCTTCAG
TGTGAAAGGCCACGTGAAGATGCTGCGGCTGGCACTAACTGTGACATCTATGACCTTTTTTATCATCGCACAAGCCCCTGAACCATATATTGTTATCACT
GGATTTGAAGTCACCGTTATCTTATTTTTCATACTTTTATATGTACTCAGACTTGATCGATTAATGAAGTGGTTATTTTGGCCTTTGCTTGATATTATCA
ACTCACTGGTAACAACAGTATTCATGCTCATCGTATCTGTGTTGGCACTGATACCAGAAACCACAACATTGACAGTTGGTGGAGGGGTGTTTGCACTTGT
GACAGCAGTATGCTGTCTTGCCGACGGGGCCCTTATTTACCGGAAGCTTCTGTTCAATCCCAGCGGTCCTTACCAGAAAAAGCCTGTGCATGAAAAAAAA
GAAGTTTTGTAATTTTATATTACTTTTTAGTTTGATACTAAGTATTAAACATATTTCTGTATTCTTCCA
- refseq_CKLF.F3 (forward primer)
- refseq_CKLF.R3 (reverse primer)
- Single exon skipping
Frame 0
MRAREREAELGEK*GRAVLRRGGGCYRFAEPTQAAS*EELRESAAAGSADAMDNVQPKIKHRPFCFS
VKGHVKMLRLALTVTSMTFFIIAQAPEPYIVIT
GFEVTVILFFILLYVLRLDRLMKWLFWPLLDII
NSLVTTVFMLIVSVLALIPETTTLTVGGGVFALV
TAVCCLADGALIYRKLLFNPSGPYQKKPVHEKK
EVL*FYITF*FDTKY*TYFCILP
Frame 1
CAQESGKPSWARSRGGRCSAAVAVAIASQNLLRQPAEKS*GKVLLLGLQTRWITCSRK*NIAPSAS
V*KAT*RCCGWH*L*HL*PFLSSHKPLNHILLSL
DLKSPLSYFSYFYMYSDLID**SGYFGLCLILS
THW*QQYSCSSYLCWH*YQKPQH*QLVEGCLHL
*QQYAVLPTGPLFTGSFCSIPAVLTRKSLCMKKK
KFCNFILLFSLILSIKHISVFF
Frame 2
ARKRAGSRAGREVGEGGAPPRWRLLSLRRTYSGSQLRRVEGKCCCWVCRRDG*RAAENKTSPLLLQ
CERPREDAAAGTNCDIYDLFYHRTSP*TIYCYH
WI*SHRYLIFHTFICTQT*SINEVVILAFA*YYQ
LTGNNSIHAHRICVGTDTRNHNIDSWWRGVCTC
DSSMLSCRRGPYLPEASVQSQRSLPEKACA*KK
RSFVILYYFLV*Y*VLNIFLYSS
Sequence NM_001040138
ATGCGCGCAAGAGAGCGGGAAGCCGAGCTGGGCGAGAAGTAGGGGAGGGCGGTGCTCCGCCGCGGTGGCGGTTGCTATCGCTTCGCAGAACCTACTCAGGCAGCCAGCTGAGAAGAGTTGAGGGAAAGTGCTGCTGCTGGGTCTGCAGACGCGATGGATAACGTGCAGCCGAAAATAAAACATCGCCCCTTCTGCTTCAG
TGTGAAAGGCCACGTGAAGATGCTGCGGCTGGCACTAACTGTGACATCTATGACCTTTTTTATCATCGCACAAGCCCCTGAACCATATATTGTTATCACT
GGATTTGAAGTCACCGTTATCTTATTTTTCATACTTTTATATGTACTCAGACTTGATCGATTAATGAAGTGGTTATTTTGGCCTTTGCTTGATATTATCA
ACTCACTGGTAACAACAGTATTCATGCTCATCGTATCTGTGTTGGCACTGATACCAGAAACCACAACATTGACAGTTGGTGGAGGGTTATTTTAACTCAG
TATAGGAGCTAGAGGAAGAGATTTCCGAAGTCTGCACCCCGCGCAGAGCACTACTGTAACTTCCAAGGGAGCGCTGGGAGCAGCGGGATCGGGTTTTCCG
GCACCCGGGCCTGGGTGGCAGGGAAGAATGTGCCGGGATCCGCCTCAGGGATCTT
- refseq_CKLF.F3 (forward primer)
- refseq_CKLF.R3 (reverse primer)
- Single exon skipping
Frame 0
MRAREREAELGEK*GRAVLRRGGGCYRFAEPTQAAS*EELRESAAAGSADAMDNVQPKIKHRPFCFS
VKGHVKMLRLALTVTSMTFFIIAQAPEPYIVIT
GFEVTVILFFILLYVLRLDRLMKWLFWPLLDII
NSLVTTVFMLIVSVLALIPETTTLTVGGGLF*LS
IGARGRDFRSLHPAQSTTVTSKGALGAAGSGFP
APGPGWQGRMCRDPPQGS
Frame 1
CAQESGKPSWARSRGGRCSAAVAVAIASQNLLRQPAEKS*GKVLLLGLQTRWITCSRK*NIAPSAS
V*KAT*RCCGWH*L*HL*PFLSSHKPLNHILLSL
DLKSPLSYFSYFYMYSDLID**SGYFGLCLILS
THW*QQYSCSSYLCWH*YQKPQH*QLVEGYFNS
V*ELEEEISEVCTPRRALL*LPRERWEQRDRVFR
HPGLGGREECAGIRLRDL
Frame 2
ARKRAGSRAGREVGEGGAPPRWRLLSLRRTYSGSQLRRVEGKCCCWVCRRDG*RAAENKTSPLLLQ
CERPREDAAAGTNCDIYDLFYHRTSP*TIYCYH
WI*SHRYLIFHTFICTQT*SINEVVILAFA*YYQ
LTGNNSIHAHRICVGTDTRNHNIDSWWRVILTQ
YRS*RKRFPKSAPRAEHYCNFQGSAGSSGIGFS
GTRAWVAGKNVPGSASGI
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