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Sequences

Sequence NM_001037631

GCCTTCTGTGTGTGCACATGTGTAATACATATCTGGGATCAAAGCTATCTATATAAAGTCCTTGATTCTGTGTGGGTTCAAACACATTTCAAAGCTTCAG
GATCCTGAAAGGTTTTGCTCTACTTCCTGAAGACCTGAACACCGCTCCCATAAAGCCATGGCTTGCCTTGGATTTCAGCGGCACAAGGCTCAGCTGAACC
TGGCTACCAGGACCTGGCCCTGCACTCTCCTGTTTTTTCTTCTCTTCATCCCTGTCTTCTGCAAAGCAATGCACGTGGCCCAGCCTGCTGTGGTACTGGC
CAGCAGCCGAGGCATCGCCAGCTTTGTGTGTGAGTATGCATCTCCAGGCAAAGCCACTGAGGTCCGGGTGACAGTGCTTCGGCAGGCTGACAGCCAGGTG
ACTGAAGTCTGTGCGGCAACCTACATGATGGGGAATGAGTTGACCTTCCTAGATGATTCCATCTGCACGGGCACCTCCAGTGGAAATCAAGTGAACCTCA
CTATCCAAGGACTGAGGGCCATGGACACGGGACTCTACATCTGCAAGGTGGAGCTCATGTACCCACCGCCATACTACCTGGGCATAGGCAACGGAACCCA
GATTTATGTAATTGCTAAAGAAAAGAAGCCCTCTTACAACAGGGGTCTATGTGAAAATGCCCCCAACAGAGCCAGAATGTGAAAAGCAATTTCAGCCTTA
TTTTATTCCCATCAATTGAGAAACCATTATGAAGAAGAGAGTCCATATTTCAATTTCCAAGAGCTGAGGCAATTCTAACTTTTTTGCTATCCAGCTATTT
TTATTTGTTTGTGCATTTGGGGGGAATTCATCTCTCTTTAATATAAAGTTGGATGCGGAACCCAAATTACGTGTACTACAATTTAAAGCAAAGGAGTAGA
AAGACAGAGCTGGGATGTTTCTGTCACATCAGCTCCACTTTCAGTGAAAGCATCACTTGGGATTAATATGGGGATGCAGCATTATGATGTGGGTCAAGGA
ATTAAGTTAGGGAATGGCACAGCCCAAAGAAGGAAAAGGCAGGGAGCGAGGGAGAAGACTATATTGTACACACCTTATATTTACGTATGAGACGTTTATA
GCCGAAATGATCTTTTCAAGTTAAATTTTATGCCTTTTATTTCTTAAACAAATGTATGATTACATCAAGGCTTCAAAAATACTCACATGGCTATGTTTTA
GCCAGTGATGCTAAAGGTTGTATTGCATATATACATATATATATATATATATATATATATATATATATATATATATATATATATATATATTTTAATTTGA
TAGTATTGTGCATAGAGCCACGTATGTTTTTGTGTATTTGTTAATGGTTTGAATATAAACACTATATGGCAGTGTCTTTCCACCTTGGGTCCCAGGGAAG
TTTTGTGGAGGAGCTCAGGACACTAATACACCAGGTAGAACACAAGGTCATTTGCTAACTAGCTTGGAAACTGGATGAGGTCATAGCAGTGCTTGATTGC
GTGGAATTGTGCTGAGTTGGTGTTGACATGTGCTTTGGGGCTTTTACACCAGTTCCTTTCAATGGTTTGCAAGGAAGCCACAGCTGGTGGTATCTGAGTT
GACTTGACAGAACACTGTCTTGAAGACAATGGCTTACTCCAGGAGACCCACAGGTATGACCTTCTAGGAAGCTCCAGTTCGATGGGCCCAATTCTTACAA
ACATGTGGTTAATGCCATGGACAGAAGAAGGCAGCAGGTGGCAGAATGGGGTGCATGAAGGTTTCTGAAAATTAACACTGCTTGTGTTTTTAACTCAATA
TTTTCCATGAAAATGCAACAACATGTATAATATTTTTAATTAAATAAAAATCTGTGGTGGTCGTTTT
  • refseq_CTLA4.F2 (forward primer)
  • refseq_CTLA4.R2 (reverse primer)
  • Single exon skipping

Frame 0

AFCVCTCVIHIWDQSYLYKVLDSVWVQTHFKAS
GS*KVLLYFLKT*TPLP*SHGLPWISAAQGSAEP
GYQDLALHSPVFSSLHPCLLQSNARGPACCGTG
QQPRHRQLCV*VCISRQSH*GPGDSASAG*QPG
D*SLCGNLHDGE*VDLPR*FHLHGHLQWKSSEPH
YPRTEGHGHGTLHLQGGAHVPTAILPGHRQRNP
DLCNC*RKEALLQQGSM*KCPQQSQNVKSNFSL
ILFPSIEKPL*RRESIFQFPRAEAILTFLLSSYF
YLFVHLGGIHLSLI*SWMRNPNYVYYNLKQRSR
KTELGCFCHISSTFSESITWD*YGDAAL*CGSR
N*VREWHSPKKEKAGSEGEDYIVHTLYLRMRRL*
PK*SFQVKFYAFYFLNKCMITSRLQKYSHGYVL
ASDAKGCIAYIHIYIYIYIYIYIYIYIYIYFNL
IVLCIEPRMFLCIC*WFEYKHYMAVSFHLGSQGS
FVEELRTLIHQVEHKVIC*LAWKLDEVIAVLDC
VELC*VGVDMCFGAFTPVPFNGLQGSHSWWYLS
*LDRTLS*RQWLTPGDPQV*PSRKLQFDGPNSYK
HVVNAMDRRRQQVAEWGA*RFLKINTACVFNSI
FSMKMQQHV*YF*LNKNLWWSF

Frame 1

PSVCAHV*YISGIKAIYIKSLILCGFKHISKLQ
DPERFCSTS*RPEHRSHKAMACLGFQRHKAQLN
LATRTWPCTLLFFLLFIPVFCKAMHVAQPAVVLA
SSRGIASFVCEYASPGKATEVRVTVLRQADSQV
TEVCAATYMMGNELTFLDDSICTGTSSGNQVNL
TIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQ
IYVIAKEKKPSYNRGLCENAPNRARM*KAISAL
FYSHQLRNHYEEESPYFNFQELRQF*LFCYPAI
FICLCIWGEFISL*YKVGCGTQITCTTI*SKGVE
RQSWDVSVTSAPLSVKASLGINMGMQHYDVGQG
IKLGNGTAQRRKRQGAREKTILYTPYIYV*DVY
SRNDLFKLNFMPFIS*TNV*LHQGFKNTHMAMF*
PVMLKVVLHIYIYIYIYIYIYIYIYIYIYILI*
*YCA*SHVCFCVFVNGLNINTIWQCLSTLGPRE
VLWRSSGH*YTR*NTRSFAN*LGNWMRS*QCLIA
WNCAELVLTCALGLLHQFLSMVCKEATAGGI*V
DLTEHCLEDNGLLQETHRYDLLGSSSSMGPILT
NMWLMPWTEEGSRWQNGVHEGF*KLTLLVFLTQY
FP*KCNNMYNIFN*IKICGGRF

Frame 2

LLCVHMCNTYLGSKLSI*SP*FCVGSNTFQSFR
ILKGFALLPEDLNTAPIKPWLALDFSGTRLS*T
WLPGPGPALSCFFFSSSLSSAKQCTWPSLLWYW
PAAEASPALCVSMHLQAKPLRSG*QCFGRLTAR*
LKSVRQPT*WGMS*PS*MIPSARAPPVEIK*TS
LSKD*GPWTRDSTSARWSSCTHRHTTWA*ATEP
RFM*LLKKRSPLTTGVYVKMPPTEPECEKQFQPY
FIPIN*ETIMKKRVHISISKS*GNSNFFAIQLF
LFVCAFGGNSSLFNIKLDAEPKLRVLQFKAKE*
KDRAGMFLSHQLHFQ*KHHLGLIWGCSIMMWVKE
LS*GMAQPKEGKGRERGRRLYCTHLIFTYETFI
AEMIFSS*ILCLLFLKQMYDYIKASKILTWLCF
SQ*C*RLYCIYTYIYIYIYIYIYIYIYIYIF*FD
SIVHRATYVFVYLLMV*I*TLYGSVFPPWVPGK
FCGGAQDTNTPGRTQGHLLTSLETG*GHSSA*L
RGIVLSWC*HVLWGFYTSSFQWFARKPQLVVSEL
T*QNTVLKTMAYSRRPTGMTF*EAPVRWAQFLQ
TCG*CHGQKKAAGGRMGCMKVSEN*HCLCF*LN
IFHENATTCIIFLIK*KSVVVV

Sequence NM_005214

GCCTTCTGTGTGTGCACATGTGTAATACATATCTGGGATCAAAGCTATCTATATAAAGTCCTTGATTCTGTGTGGGTTCAAACACATTTCAAAGCTTCAG
GATCCTGAAAGGTTTTGCTCTACTTCCTGAAGACCTGAACACCGCTCCCATAAAGCCATGGCTTGCCTTGGATTTCAGCGGCACAAGGCTCAGCTGAACC
TGGCTACCAGGACCTGGCCCTGCACTCTCCTGTTTTTTCTTCTCTTCATCCCTGTCTTCTGCAAAGCAATGCACGTGGCCCAGCCTGCTGTGGTACTGGC
CAGCAGCCGAGGCATCGCCAGCTTTGTGTGTGAGTATGCATCTCCAGGCAAAGCCACTGAGGTCCGGGTGACAGTGCTTCGGCAGGCTGACAGCCAGGTG
ACTGAAGTCTGTGCGGCAACCTACATGATGGGGAATGAGTTGACCTTCCTAGATGATTCCATCTGCACGGGCACCTCCAGTGGAAATCAAGTGAACCTCA
CTATCCAAGGACTGAGGGCCATGGACACGGGACTCTACATCTGCAAGGTGGAGCTCATGTACCCACCGCCATACTACCTGGGCATAGGCAACGGAACCCA
GATTTATGTAATTGATCCAGAACCGTGCCCAGATTCTGACTTCCTCCTCTGGATCCTTGCAGCAGTTAGTTCGGGGTTGTTTTTTTATAGCTTTCTCCTC
ACAGCTGTTTCTTTGAGCAAAATGCTAAAGAAAAGAAGCCCTCTTACAACAGGGGTCTATGTGAAAATGCCCCCAACAGAGCCAGAATGTGAAAAGCAAT
TTCAGCCTTATTTTATTCCCATCAATTGAGAAACCATTATGAAGAAGAGAGTCCATATTTCAATTTCCAAGAGCTGAGGCAATTCTAACTTTTTTGCTAT
CCAGCTATTTTTATTTGTTTGTGCATTTGGGGGGAATTCATCTCTCTTTAATATAAAGTTGGATGCGGAACCCAAATTACGTGTACTACAATTTAAAGCA
AAGGAGTAGAAAGACAGAGCTGGGATGTTTCTGTCACATCAGCTCCACTTTCAGTGAAAGCATCACTTGGGATTAATATGGGGATGCAGCATTATGATGT
GGGTCAAGGAATTAAGTTAGGGAATGGCACAGCCCAAAGAAGGAAAAGGCAGGGAGCGAGGGAGAAGACTATATTGTACACACCTTATATTTACGTATGA
GACGTTTATAGCCGAAATGATCTTTTCAAGTTAAATTTTATGCCTTTTATTTCTTAAACAAATGTATGATTACATCAAGGCTTCAAAAATACTCACATGG
CTATGTTTTAGCCAGTGATGCTAAAGGTTGTATTGCATATATACATATATATATATATATATATATATATATATATATATATATATATATATATATATAT
TTTAATTTGATAGTATTGTGCATAGAGCCACGTATGTTTTTGTGTATTTGTTAATGGTTTGAATATAAACACTATATGGCAGTGTCTTTCCACCTTGGGT
CCCAGGGAAGTTTTGTGGAGGAGCTCAGGACACTAATACACCAGGTAGAACACAAGGTCATTTGCTAACTAGCTTGGAAACTGGATGAGGTCATAGCAGT
GCTTGATTGCGTGGAATTGTGCTGAGTTGGTGTTGACATGTGCTTTGGGGCTTTTACACCAGTTCCTTTCAATGGTTTGCAAGGAAGCCACAGCTGGTGG
TATCTGAGTTGACTTGACAGAACACTGTCTTGAAGACAATGGCTTACTCCAGGAGACCCACAGGTATGACCTTCTAGGAAGCTCCAGTTCGATGGGCCCA
ATTCTTACAAACATGTGGTTAATGCCATGGACAGAAGAAGGCAGCAGGTGGCAGAATGGGGTGCATGAAGGTTTCTGAAAATTAACACTGCTTGTGTTTT
TAACTCAATATTTTCCATGAAAATGCAACAACATGTATAATATTTTTAATTAAATAAAAATCTGTGGTGGTCGTTTT
  • refseq_CTLA4.F2 (forward primer)
  • refseq_CTLA4.R2 (reverse primer)
  • Single exon skipping

Frame 0

AFCVCTCVIHIWDQSYLYKVLDSVWVQTHFKAS
GS*KVLLYFLKT*TPLP*SHGLPWISAAQGSAEP
GYQDLALHSPVFSSLHPCLLQSNARGPACCGTG
QQPRHRQLCV*VCISRQSH*GPGDSASAG*QPG
D*SLCGNLHDGE*VDLPR*FHLHGHLQWKSSEPH
YPRTEGHGHGTLHLQGGAHVPTAILPGHRQRNP
DLCN*SRTVPRF*LPPLDPCSS*FGVVFL*LSP
HSCFFEQNAKEKKPSYNRGLCENAPNRARM*KAI
SALFYSHQLRNHYEEESPYFNFQELRQF*LFCY
PAIFICLCIWGEFISL*YKVGCGTQITCTTI*S
KGVERQSWDVSVTSAPLSVKASLGINMGMQHYDV
GQGIKLGNGTAQRRKRQGAREKTILYTPYIYV*
DVYSRNDLFKLNFMPFIS*TNV*LHQGFKNTHM
AMF*PVMLKVVLHIYIYIYIYIYIYIYIYIYIYI
LI**YCA*SHVCFCVFVNGLNINTIWQCLSTLG
PREVLWRSSGH*YTR*NTRSFAN*LGNWMRS*Q
CLIAWNCAELVLTCALGLLHQFLSMVCKEATAGG
I*VDLTEHCLEDNGLLQETHRYDLLGSSSSMGP
ILTNMWLMPWTEEGSRWQNGVHEGF*KLTLLVF
LTQYFP*KCNNMYNIFN*IKICGGRF

Frame 1

PSVCAHV*YISGIKAIYIKSLILCGFKHISKLQ
DPERFCSTS*RPEHRSHKAMACLGFQRHKAQLN
LATRTWPCTLLFFLLFIPVFCKAMHVAQPAVVLA
SSRGIASFVCEYASPGKATEVRVTVLRQADSQV
TEVCAATYMMGNELTFLDDSICTGTSSGNQVNL
TIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQ
IYVIDPEPCPDSDFLLWILAAVSSGLFFYSFLL
TAVSLSKMLKKRSPLTTGVYVKMPPTEPECEKQ
FQPYFIPIN*ETIMKKRVHISISKS*GNSNFFAI
QLFLFVCAFGGNSSLFNIKLDAEPKLRVLQFKA
KE*KDRAGMFLSHQLHFQ*KHHLGLIWGCSIMM
WVKELS*GMAQPKEGKGRERGRRLYCTHLIFTYE
TFIAEMIFSS*ILCLLFLKQMYDYIKASKILTW
LCFSQ*C*RLYCIYTYIYIYIYIYIYIYIYIYI
F*FDSIVHRATYVFVYLLMV*I*TLYGSVFPPWV
PGKFCGGAQDTNTPGRTQGHLLTSLETG*GHSS
A*LRGIVLSWC*HVLWGFYTSSFQWFARKPQLV
VSELT*QNTVLKTMAYSRRPTGMTF*EAPVRWAQ
FLQTCG*CHGQKKAAGGRMGCMKVSEN*HCLCF
*LNIFHENATTCIIFLIK*KSVVVV

Frame 2

LLCVHMCNTYLGSKLSI*SP*FCVGSNTFQSFR
ILKGFALLPEDLNTAPIKPWLALDFSGTRLS*T
WLPGPGPALSCFFFSSSLSSAKQCTWPSLLWYW
PAAEASPALCVSMHLQAKPLRSG*QCFGRLTAR*
LKSVRQPT*WGMS*PS*MIPSARAPPVEIK*TS
LSKD*GPWTRDSTSARWSSCTHRHTTWA*ATEP
RFM*LIQNRAQILTSSSGSLQQLVRGCFFIAFSS
QLFL*AKC*RKEALLQQGSM*KCPQQSQNVKSN
FSLILFPSIEKPL*RRESIFQFPRAEAILTFLL
SSYFYLFVHLGGIHLSLI*SWMRNPNYVYYNLKQ
RSRKTELGCFCHISSTFSESITWD*YGDAAL*C
GSRN*VREWHSPKKEKAGSEGEDYIVHTLYLRM
RRL*PK*SFQVKFYAFYFLNKCMITSRLQKYSHG
YVLASDAKGCIAYIHIYIYIYIYIYIYIYIYIY
FNLIVLCIEPRMFLCIC*WFEYKHYMAVSFHLG
SQGSFVEELRTLIHQVEHKVIC*LAWKLDEVIAV
LDCVELC*VGVDMCFGAFTPVPFNGLQGSHSWW
YLS*LDRTLS*RQWLTPGDPQV*PSRKLQFDGP
NSYKHVVNAMDRRRQQVAEWGA*RFLKINTACVF
NSIFSMKMQQHV*YF*LNKNLWWSF

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