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Sequences

Sequence NM_000079

GTGCCAGTGAGAAGCACAGGCCACCACTCTGCCCTGGTCCACACAAGCTCCGGTAGCCCATGGAGCCCTGGCCTCTCCTCCTGCTCTTTAGCCTTTGCTC AGCTGGCCTCGTCCTGGGCTCCGAACATGAGACCCGTCTGGTGGCAAAGCTATTTAAAGACTACAGCAGCGTGGTGCGGCCAGTGGAAGACCACCGCCAG GTCGTGGAGGTCACCGTGGGCCTGCAGCTGATACAGCTCATCAATGTGGATGAAGTAAATCAGATCGTGACAACCAATGTGCGTCTGAAACAGCAATGGG TGGATTACAACCTAAAATGGAATCCAGATGACTATGGCGGTGTGAAAAAAATTCACATTCCTTCAGAAAAGATCTGGCGCCCAGACCTTGTTCTCTATAA CAATGCAGATGGTGACTTTGCTATTGTCAAGTTCACCAAAGTGCTCCTGCAGTACACTGGCCACATCACGTGGACACCTCCAGCCATCTTTAAAAGCTAC TGTGAGATCATCGTCACCCACTTTCCCTTTGATGAACAGAACTGCAGCATGAAGCTGGGCACCTGGACCTACGACGGCTCTGTCGTGGCCATCAACCCGG AAAGCGACCAGCCAGACCTGAGCAACTTCATGGAGAGCGGGGAGTGGGTGATCAAGGAGTCCCGGGGCTGGAAGCACTCCGTGACCTATTCCTGCTGCCC CGACACCCCCTACCTGGACATCACCTACCACTTCGTCATGCAGCGCCTGCCCCTCTACTTCATCGTCAACGTCATCATCCCCTGCCTGCTCTTCTCCTTC TTAACTGGCCTGGTATTCTACCTGCCCACAGACTCAGGGGAGAAGATGACTCTGAGCATCTCTGTCTTACTGTCTTTGACTGTGTTCCTTCTGGTCATCG TGGAGCTGATCCCCTCCACGTCCAGTGCTGTGCCCTTGATTGGAAAATACATGCTGTTCACCATGGTGTTCGTCATTGCCTCCATCATCATCACTGTCAT CGTCATCAACACACACCACCGCTCACCCAGCACCCATGTCATGCCCAACTGGGTGCGGAAGGTTTTTATCGACACTATCCCAAATATCATGTTTTTCTCC ACAATGAAAAGACCATCCAGAGAAAAGCAAGACAAAAAGATTTTTACAGAAGACATTGATATCTCTGACATTTCTGGAAAGCCAGGGCCTCCACCCATGG GCTTCCACTCTCCCCTGATCAAACACCCCGAGGTGAAAAGTGCCATCGAGGGCATCAAGTACATCGCAGAGACCATGAAGTCAGACCAGGAGTCTAACAA TGCGGCGGCAGAGTGGAAGTACGTTGCAATGGTGATGGACCACATACTCCTCGGAGTCTTCATGCTTGTTTGCATCATCGGAACCCTAGCCGTGTTTGCA GGTCGACTCATTGAATTAAATCAGCAAGGATGAGCAGAAAATGAGCTGAGCTTAGCTCTGCCCTGGAACCTACCAGAGCAGAGAAGGGCAGGAGAGGAAG ATTTGTCTACTTGCTCCACTCGCACTTATCAAACGTGTTATATTCCATACTTATTATTGATGATAAGATTTACCTTTATGTAAGTTTATGGCCTTGAAGT GTTTTCATATTGCTTCTCCCTTTAGTTCTGCTGTCTCCCTGAAGAGTGAACCCTCTTTAGTAAATGAAACTAATCACTAAAAAAAGTGTTCATTTCCAGT GTCTGGAAGAGTTTTTGCCAGGATAACCGAGGTTTTCTGTTGCATTGTCATTGCTGTTATATTTATATATTTACTTATTTTGTCTAACCACGAGACCACT AGGGAGTTGCTGATGTTCTAAATGAAGTGTATTCTTTGCTTAAGAGTCAGTTTTGTACCTACTAAGAAAACCCCATCTACCAGTCCTAAAGAGATAAGGC ATTTGATTTGGAGAGAGAGGAAGCAGTAGAAATGAAATAAACTAAAATGCAAACTGCATCAAAAA

refseq_CHRNA1.F1 (forward primer)
refseq_CHRNA1.R1 (reverse primer)
Single exon skipping

Frame 0

VPVRSTGHHSALVHTSSGSPWSPGLSSCSLAFA QLASSWAPNMRPVWWQSYLKTTAAWCGQWKTTAR SWRSPWACS*YSSSMWMK*IRS*QPMCV*NSNG WITT*NGIQMTMAV*KKFTFLQKRSGAQTLFSI TMQMVTLLLSSSPKCSCSTLATSRGHLQPSLKAT VRSSSPTFPLMNRTAA*SWAPGPTTALSWPSTR KATSQT*ATSWRAGSG*SRSPGAGSTP*PIPAA PTPPTWTSPTTSSCSACPSTSSSTSSSPACSSPS *LAWYSTCPQTQGRR*L*ASLSYCL*LCSFWSS WS*SPPRPVLCP*LENTCCSPWCSSLPPSSSLS SSSTHTTAHPAPMSCPTGCGRFLSTLSQISCFSP Q*KDHPEKSKTKRFLQKTLISLTFLESQGLHPW ASTLP*SNTPR*KVPSRASSTSQRP*SQTRSLT MRRQSGSTLQW*WTTYSSESSCLFASSEP*PCLQ VDSLN*ISKDEQKMS*A*LCPGTYQSREGQERK ICLLAPLALIKRVIFHTYY***DLPLCKFMALK CFHIASPFSSAVSLKSEPSLVNETNH*KKCSFPV SGRVFARITEVFCCIVIAVIFIYLLILSNHETT RELLMF*MKCILCLRVSFVPTKKTPSTSPKEIR HLIWRERKQ*K*NKLKCKLHQK

Frame 1

CQ*EAQATTLPWSTQAPVAHGALASPPAL*PLL SWPRPGLRT*DPSGGKAI*RLQQRGAASGRPPP GRGGHRGPAADTAHQCG*SKSDRDNQCASETAMG GLQPKMESR*LWRCEKNSHSFRKDLAPRPCSL* QCRW*LCYCQVHQSAPAVHWPHHVDTSSHL*KL L*DHRHPLSL**TELQHEAGHLDLRRLCRGHQPG KRPARPEQLHGERGVGDQGVPGLEALRDLFLLP RHPLPGHHLPLRHAAPAPLLHRQRHHPLPALLL LNWPGILPAHRLRGEDDSEHLCLTVFDCVPSGHR GADPLHVQCCALDWKIHAVHHGVRHCLHHHHCH RHQHTPPLTQHPCHAQLGAEGFYRHYPKYHVFL HNEKTIQRKARQKDFYRRH*YL*HFWKARASTHG LPLSPDQTPRGEKCHRGHQVHRRDHEVRPGV*Q CGGRVEVRCNGDGPHTPRSLHACLHHRNPSRVC RSTH*IKSARMSRK*AELSSALEPTRAEKGRRGR FVYLLHSHLSNVLYSILIIDDKIYLYVSLWP*S VFILLLPLVLLSP*RVNPL**MKLITKKSVHFQ CLEEFLPG*PRFSVALSLLLYLYIYLFCLTTRPL GSC*CSK*SVFFA*ESVLYLLRKPHLPVLKR*G I*FGERGSSRNEIN*NANCIK

Frame 2

ASEKHRPPLCPGPHKLR*PMEPWPLLLLFSLCS AGLVLGSEHETRLVAKLFKDYSSVVRPVEDHRQ VVEVTVGLQLIQLINVDEVNQIVTTNVRLKQQW VDYNLKWNPDDYGGVKKIHIPSEKIWRPDLVLYN NADGDFAIVKFTKVLLQYTGHITWTPPAIFKSY CEIIVTHFPFDEQNCSMKLGTWTYDGSVVAINP ESDQPDLSNFMESGEWVIKESRGWKHSVTYSCCP DTPYLDITYHFVMQRLPLYFIVNVIIPCLLFSF LTGLVFYLPTDSGEKMTLSISVLLSLTVFLLVI VELIPSTSSAVPLIGKYMLFTMVFVIASIIITVI VINTHHRSPSTHVMPNWVRKVFIDTIPNIMFFS TMKRPSREKQDKKIFTEDIDISDISGKPGPPPM GFHSPLIKHPEVKSAIEGIKYIAETMKSDQESNN AAAEWKYVAMVMDHILLGVFMLVCIIGTLAVFA GRLIELNQQG*AENELSLALPWNLPEQRRAGEE DLSTCSTRTYQTCYIPYLLLMIRFTFM*VYGLEV FSYCFSL*FCCLPEE*TLFSK*N*SLKKVFISS VWKSFCQDNRGFLLHCHCCYIYIFTYFV*PRDH *GVADVLNEVYSLLKSQFCTY*ENPIYQS*RDKA FDLEREEAVEMK*TKMQTASK

Sequence NM_001039523

GTGCCAGTGAGAAGCACAGGCCACCACTCTGCCCTGGTCCACACAAGCTCCGGTAGCCCATGGAGCCCTGGCCTCTCCTCCTGCTCTTTAGCCTTTGCTC AGCTGGCCTCGTCCTGGGCTCCGAACATGAGACCCGTCTGGTGGCAAAGCTATTTAAAGACTACAGCAGCGTGGTGCGGCCAGTGGAAGACCACCGCCAG GTCGTGGAGGTCACCGTGGGCCTGCAGCTGATACAGCTCATCAATGTGGATGAAGTAAATCAGATCGTGACAACCAATGTGCGTCTGAAACAGGGTGACA TGGTAGATCTGCCACGCCCCAGCTGCGTGACTTTGGGAGTTCCTTTGTTTTCTCATCTGCAGAATGAGCAATGGGTGGATTACAACCTAAAATGGAATCC AGATGACTATGGCGGTGTGAAAAAAATTCACATTCCTTCAGAAAAGATCTGGCGCCCAGACCTTGTTCTCTATAACAATGCAGATGGTGACTTTGCTATT GTCAAGTTCACCAAAGTGCTCCTGCAGTACACTGGCCACATCACGTGGACACCTCCAGCCATCTTTAAAAGCTACTGTGAGATCATCGTCACCCACTTTC CCTTTGATGAACAGAACTGCAGCATGAAGCTGGGCACCTGGACCTACGACGGCTCTGTCGTGGCCATCAACCCGGAAAGCGACCAGCCAGACCTGAGCAA CTTCATGGAGAGCGGGGAGTGGGTGATCAAGGAGTCCCGGGGCTGGAAGCACTCCGTGACCTATTCCTGCTGCCCCGACACCCCCTACCTGGACATCACC TACCACTTCGTCATGCAGCGCCTGCCCCTCTACTTCATCGTCAACGTCATCATCCCCTGCCTGCTCTTCTCCTTCTTAACTGGCCTGGTATTCTACCTGC CCACAGACTCAGGGGAGAAGATGACTCTGAGCATCTCTGTCTTACTGTCTTTGACTGTGTTCCTTCTGGTCATCGTGGAGCTGATCCCCTCCACGTCCAG TGCTGTGCCCTTGATTGGAAAATACATGCTGTTCACCATGGTGTTCGTCATTGCCTCCATCATCATCACTGTCATCGTCATCAACACACACCACCGCTCA CCCAGCACCCATGTCATGCCCAACTGGGTGCGGAAGGTTTTTATCGACACTATCCCAAATATCATGTTTTTCTCCACAATGAAAAGACCATCCAGAGAAA AGCAAGACAAAAAGATTTTTACAGAAGACATTGATATCTCTGACATTTCTGGAAAGCCAGGGCCTCCACCCATGGGCTTCCACTCTCCCCTGATCAAACA CCCCGAGGTGAAAAGTGCCATCGAGGGCATCAAGTACATCGCAGAGACCATGAAGTCAGACCAGGAGTCTAACAATGCGGCGGCAGAGTGGAAGTACGTT GCAATGGTGATGGACCACATACTCCTCGGAGTCTTCATGCTTGTTTGCATCATCGGAACCCTAGCCGTGTTTGCAGGTCGACTCATTGAATTAAATCAGC AAGGATGAGCAGAAAATGAGCTGAGCTTAGCTCTGCCCTGGAACCTACCAGAGCAGAGAAGGGCAGGAGAGGAAGATTTGTCTACTTGCTCCACTCGCAC TTATCAAACGTGTTATATTCCATACTTATTATTGATGATAAGATTTACCTTTATGTAAGTTTATGGCCTTGAAGTGTTTTCATATTGCTTCTCCCTTTAG TTCTGCTGTCTCCCTGAAGAGTGAACCCTCTTTAGTAAATGAAACTAATCACTAAAAAAAGTGTTCATTTCCAGTGTCTGGAAGAGTTTTTGCCAGGATA ACCGAGGTTTTCTGTTGCATTGTCATTGCTGTTATATTTATATATTTACTTATTTTGTCTAACCACGAGACCACTAGGGAGTTGCTGATGTTCTAAATGA AGTGTATTCTTTGCTTAAGAGTCAGTTTTGTACCTACTAAGAAAACCCCATCTACCAGTCCTAAAGAGATAAGGCATTTGATTTGGAGAGAGAGGAAGCA GTAGAAATGAAATAAACTAAAATGCAAACTGCATCAAAAA

refseq_CHRNA1.F1 (forward primer)
refseq_CHRNA1.R1 (reverse primer)
Single exon skipping

Frame 0

VPVRSTGHHSALVHTSSGSPWSPGLSSCSLAFA QLASSWAPNMRPVWWQSYLKTTAAWCGQWKTTAR SWRSPWACS*YSSSMWMK*IRS*QPMCV*NRVT W*ICHAPAA*LWEFLCFLICRMSNGWITT*NGI QMTMAV*KKFTFLQKRSGAQTLFSITMQMVTLLL SSSPKCSCSTLATSRGHLQPSLKATVRSSSPTF PLMNRTAA*SWAPGPTTALSWPSTRKATSQT*A TSWRAGSG*SRSPGAGSTP*PIPAAPTPPTWTSP TTSSCSACPSTSSSTSSSPACSSPS*LAWYSTC PQTQGRR*L*ASLSYCL*LCSFWSSWS*SPPRP VLCP*LENTCCSPWCSSLPPSSSLSSSSTHTTAH PAPMSCPTGCGRFLSTLSQISCFSPQ*KDHPEK SKTKRFLQKTLISLTFLESQGLHPWASTLP*SN TPR*KVPSRASSTSQRP*SQTRSLTMRRQSGSTL QW*WTTYSSESSCLFASSEP*PCLQVDSLN*IS KDEQKMS*A*LCPGTYQSREGQERKICLLAPLA LIKRVIFHTYY***DLPLCKFMALKCFHIASPFS SAVSLKSEPSLVNETNH*KKCSFPVSGRVFARI TEVFCCIVIAVIFIYLLILSNHETTRELLMF*M KCILCLRVSFVPTKKTPSTSPKEIRHLIWRERKQ *K*NKLKCKLHQK

Frame 1

CQ*EAQATTLPWSTQAPVAHGALASPPAL*PLL SWPRPGLRT*DPSGGKAI*RLQQRGAASGRPPP GRGGHRGPAADTAHQCG*SKSDRDNQCASETG*H GRSATPQLRDFGSSFVFSSAE*AMGGLQPKMES R*LWRCEKNSHSFRKDLAPRPCSL*QCRW*LCY CQVHQSAPAVHWPHHVDTSSHL*KLL*DHRHPLS L**TELQHEAGHLDLRRLCRGHQPGKRPARPEQ LHGERGVGDQGVPGLEALRDLFLLPRHPLPGHH LPLRHAAPAPLLHRQRHHPLPALLLLNWPGILPA HRLRGEDDSEHLCLTVFDCVPSGHRGADPLHVQ CCALDWKIHAVHHGVRHCLHHHHCHRHQHTPPL TQHPCHAQLGAEGFYRHYPKYHVFLHNEKTIQRK ARQKDFYRRH*YL*HFWKARASTHGLPLSPDQT PRGEKCHRGHQVHRRDHEVRPGV*QCGGRVEVR CNGDGPHTPRSLHACLHHRNPSRVCRSTH*IKSA RMSRK*AELSSALEPTRAEKGRRGRFVYLLHSH LSNVLYSILIIDDKIYLYVSLWP*SVFILLLPL VLLSP*RVNPL**MKLITKKSVHFQCLEEFLPG* PRFSVALSLLLYLYIYLFCLTTRPLGSC*CSK* SVFFA*ESVLYLLRKPHLPVLKR*GI*FGERGS SRNEIN*NANCIK

Frame 2

ASEKHRPPLCPGPHKLR*PMEPWPLLLLFSLCS AGLVLGSEHETRLVAKLFKDYSSVVRPVEDHRQ VVEVTVGLQLIQLINVDEVNQIVTTNVRLKQGD MVDLPRPSCVTLGVPLFSHLQNEQWVDYNLKWNP DDYGGVKKIHIPSEKIWRPDLVLYNNADGDFAI VKFTKVLLQYTGHITWTPPAIFKSYCEIIVTHF PFDEQNCSMKLGTWTYDGSVVAINPESDQPDLSN FMESGEWVIKESRGWKHSVTYSCCPDTPYLDIT YHFVMQRLPLYFIVNVIIPCLLFSFLTGLVFYL PTDSGEKMTLSISVLLSLTVFLLVIVELIPSTSS AVPLIGKYMLFTMVFVIASIIITVIVINTHHRS PSTHVMPNWVRKVFIDTIPNIMFFSTMKRPSRE KQDKKIFTEDIDISDISGKPGPPPMGFHSPLIKH PEVKSAIEGIKYIAETMKSDQESNNAAAEWKYV AMVMDHILLGVFMLVCIIGTLAVFAGRLIELNQ QG*AENELSLALPWNLPEQRRAGEEDLSTCSTRT YQTCYIPYLLLMIRFTFM*VYGLEVFSYCFSL* FCCLPEE*TLFSK*N*SLKKVFISSVWKSFCQD NRGFLLHCHCCYIYIFTYFV*PRDH*GVADVLNE VYSLLKSQFCTY*ENPIYQS*RDKAFDLEREEA VEMK*TKMQTASK