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Sequences

Sequence NM_001006628

AATCTTTTCCTTTTCTTTTGCAAGATCAAGGGAGAAAGAGAACCGGCAGCTGGCCTCGGACTCTAAGCGGTGCGCAGCTCCAGCCCGAGCGGATCGGCCC
TGAACCCACAAAGGACTCCTCGCTCCTTCAAGCCTCCACCACCTCGCAGCCGGGGAGGCAACTGGAGCGAAACCAGCGACAGGTTGTTAGCGACATGAGT
ACAGGGTCTGGATCTTACCCATTCAAGAGTGTATTCTCAGCACCCAATGCCTGGCATATAGTTTAAATGTTTATTTGCTACTTGGCTACTGATTAGAGAA
CGCAAAATGAATAACTCAACAAACTCCTCTAACAATAGCCTGGCTCTTACAAGTCCTTATAAGACATTTGAAGTGGTGTTTATTGTCCTGGTGGCTGGAT
CCCTCAGTTTGGTGACCATTATCGGGAACATCCTAGTCATGGTTTCCATTAAAGTCAACCGCCACCTCCAGACCGTCAACAATTACTTTTTATTCAGCTT
GGCCTGTGCTGACCTTATCATAGGTGTTTTCTCCATGAACTTGTACACCCTCTACACTGTGATTGGTTACTGGCCTTTGGGACCTGTGGTGTGTGACCTT
TGGCTAGCCCTGGACTATGTGGTCAGCAATGCCTCAGTTATGAATCTGCTCATCATCAGCTTTGACAGGTACTTCTGTGTCACAAAACCTCTGACCTACC
CAGTCAAGCGGACCACAAAAATGGCAGGTATGATGATTGCAGCTGCCTGGGTCCTCTCTTTCATCCTCTGGGCTCCAGCCATTCTCTTCTGGCAGTTCAT
TGTAGGGGTGAGAACTGTGGAGGATGGGGAGTGCTACATTCAGTTTTTTTCCAATGCTGCTGTCACCTTTGGTACGGCTATTGCAGCCTTCTATTTGCCA
GTGATCATCATGACTGTGCTATATTGGCACATATCCCGAGCCAGCAAGAGCAGGATAAAGAAGGACAAGAAGGAGCCTGTTGCCAACCAAGACCCCGTTT
CTCCAAGTCTGGTACAAGGAAGGATAGTGAAGCCAAACAATAACAACATGCCCAGCAGTGACGATGGCCTGGAGCACAACAAAATCCAGAATGGCAAAGC
CCCCAGGGATCCTGTGACTGAAAACTGTGTTCAGGGAGAGGAGAAGGAGAGCTCCAATGACTCCACCTCAGTCAGTGCTGTTGCCTCTAATATGAGAGAT
GATGAAATAACCCAGGATGAAAACACAGTTTCCACTTCCCTGGGCCATTCCAAAGATGAGAACTCTAAGCAAACATGCATCAGAATTGGCACCAAGACCC
CAAAAAGTGACTCATGTACCCCAACTAATACCACCGTGGAGGTAGTGGGGTCTTCAGGTCAGAATGGAGATGAAAAGCAGAATATTGTAGCCCGCAAGAT
TGTGAAGATGACTAAGCAGCCTGCAAAAAAGAAGCCTCCTCCTTCCCGGGAAAAGAAAGTCACCAGGACAATCTTGGCTATTCTGTTGGCTTTCATCATC
ACTTGGGCCCCATACAATGTCATGGTGCTCATTAACACCTTTTGTGCACCTTGCATCCCCAACACTGTGTGGACAATTGGTTACTGGCTTTGTTACATCA
ACAGCACTATCAACCCTGCCTGCTATGCACTTTGCAATGCCACCTTCAAGAAGACCTTTAAACACCTTCTCATGTGTCATTATAAGAACATAGGCGCTAC
AAGGTAAAATATCTTTGAAAAAGATAGAAGGTGGGCAAGGGGAGCTTGAGAAGAATAAAAGGGATAAACGAGCTCCTAGTTTTAAAATCTCTGCCATTGC
ACTTTATAGTCTGATTACAAAACGTGCAATTCAGGAGCCCAGCAGTGACACACTTATCACGCCTAGGCTCCAGTTTGCAAAAATTGCACCTTATAAACTG
TCAGTATTAGGAGCAATGAGACAATGAAAGAAACATGTTGGGATCGTGGATTTAAGAAACTATACACTGTTTCTCATAATCTCTTGAAGAAGGGCTTCTG
ATTCTACAATTTTATCAGTCTCTGCACAAGAGGAATAACCTTGTTCCTTTTTTGTTACTTTTGTTGTTGTTGTTCTCATGTGTCCTTAAGAGAAGGAAAT
GCCACAGTTACAAGGTAAACATGGAGACTTAAACATAAAGAAATAGGCACTATACAATGGGGACATAAAAAAAGAAAATCAAAGAAGGATGCAGAAATTG
TCTCCGGAGTGTTAAGCATATTTTATTCTTTTGTTACGGTCCTATTTAGAGGATTGGAATGTAATAAATGCTTATTTTTTGCCTTTCTTTTTCCCACCAT
GAAGAGAAAGCAAACAAACAGAAACCCCAACTAGGTCACACCATTTTTCTTCTTGTTATGCCACTATTTTGTACATCCCTGTTCTATATTGTTTATAGGG
AAAACCTACAGGACTTTCACTAAAGCTAGCCAGAGACAGCCATGAAAATGGACGTTTCACCCCTC
  • refseq_CHRM2.F3 (forward primer)
  • refseq_CHRM2.R3 (reverse primer)
  • Single exon skipping

Frame 0

NLFLFFCKIKGEREPAAGLGL*AVRSSSPSGSA
LNPQRTPRSFKPPPPRSRGGNWSETSDRLLAT*V
QGLDLTHSRVYSQHPMPGI*FKCLFATWLLIRE
RKMNNSTNSSNNSLALTSPYKTFEVVFIVLVAG
SLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSL
ACADLIIGVFSMNLYTLYTVIGYWPLGPVVCDL
WLALDYVVSNASVMNLLIISFDRYFCVTKPLTY
PVKRTTKMAGMMIAAAWVLSFILWAPAILFWQFI
VGVRTVEDGECYIQFFSNAAVTFGTAIAAFYLP
VIIMTVLYWHISRASKSRIKKDKKEPVANQDPV
SPSLVQGRIVKPNNNNMPSSDDGLEHNKIQNGKA
PRDPVTENCVQGEEKESSNDSTSVSAVASNMRD
DEITQDENTVSTSLGHSKDENSKQTCIRIGTKT
PKSDSCTPTNTTVEVVGSSGQNGDEKQNIVARKI
VKMTKQPAKKKPPPSREKKVTRTILAILLAFII
TWAPYNVMVLINTFCAPCIPNTVWTIGYWLCYI
NSTINPACYALCNATFKKTFKHLLMCHYKNIGAT
R*NIFEKDRRWARGA*EE*KG*TSS*F*NLCHC
TL*SDYKTCNSGAQQ*HTYHA*APVCKNCTL*T
VSIRSNETMKETCWDRGFKKLYTVSHNLLKKGF*
FYNFISLCTRGITLFLFCYFCCCCSHVSLREGN
ATVTR*TWRLKHKEIGTIQWGHKKRKSKKDAEI
VSGVLSIFYSFVTVLFRGLECNKCLFFAFLFPTM
KRKQTNRNPN*VTPFFFLLCHYFVHPCSILFIG
KTYRTFTKASQRQP*KWTFHP

Frame 1

IFSFSFARSREKENRQLASDSKRCAAPARADRP
*THKGLLAPSSLHHLAAGEATGAKPATGC*RHE
YRVWILPIQECILSTQCLAYSLNVYLLLGY*LEN
AK*ITQQTPLTIAWLLQVLIRHLKWCLLSWWLD
PSVW*PLSGTS*SWFPLKSTATSRPSTITFYSA
WPVLTLS*VFSP*TCTPSTL*LVTGLWDLWCVTF
G*PWTMWSAMPQL*ICSSSALTGTSVSQNL*PT
QSSGPQKWQV**LQLPGSSLSSSGLQPFSSGSS
L*G*ELWRMGSATFSFFPMLLSPLVRLLQPSICQ
*SS*LCYIGTYPEPARAG*RRTRRSLLPTKTPF
LQVWYKEG**SQTITTCPAVTMAWSTTKSRMAK
PPGIL*LKTVFRERRRRAPMTPPQSVLLPLI*EM
MK*PRMKTQFPLPWAIPKMRTLSKHASELAPRP
QKVTHVPQLIPPWR*WGLQVRMEMKSRIL*PAR
L*R*LSSLQKRSLLLPGKRKSPGQSWLFCWLSSS
LGPHTMSWCSLTPFVHLASPTLCGQLVTGFVTS
TALSTLPAMHFAMPPSRRPLNTFSCVIIRT*AL
QGKISLKKIEGGQGELEKNKRDKRAPSFKISAIA
LYSLITKRAIQEPSSDTLITPRLQFAKIAPYKL
SVLGAMRQ*KKHVGIVDLRNYTLFLIIS*RRAS
DSTILSVSAQEE*PCSFFVTFVVVVLMCP*EKEM
PQLQGKHGDLNIKK*ALYNGDIKKENQRRMQKL
SPEC*AYFILLLRSYLEDWNVINAYFLPFFFPP
*RESKQTETPTRSHHFSSCYATILYIPVLYCL*G
KPTGLSLKLARDSHENGRFTP

Frame 2

SFPFLLQDQGRKRTGSWPRTLSGAQLQPERIGP
EPTKDSSLLQASTTSQPGRQLERNQRQVVSDMS
TGSGSYPFKSVFSAPNAWHIV*MFICYLATD*R
TQNE*LNKLL*Q*PGSYKSL*DI*SGVYCPGGWI
PQFGDHYREHPSHGFH*SQPPPPDRQQLLFIQL
GLC*PYHRCFLHELVHPLHCDWLLAFGTCGV*P
LASPGLCGQQCLSYESAHHQL*QVLLCHKTSDLP
SQADHKNGRYDDCSCLGPLFHPLGSSHSLLAVH
CRGENCGGWGVLHSVFFQCCCHLWYGYCSLLFA
SDHHDCAILAHIPSQQEQDKEGQEGACCQPRPRF
SKSGTRKDSEAKQ*QHAQQ*RWPGAQQNPEWQS
PQGSCD*KLCSGRGEGELQ*LHLSQCCCL*YER
**NNPG*KHSFHFPGPFQR*EL*ANMHQNWHQDP
KK*LMYPN*YHRGGSGVFRSEWR*KAEYCSPQD
CEDD*AACKKEASSFPGKESHQDNLGYSVGFHH
HLGPIQCHGAH*HLLCTLHPQHCVDNWLLALLHQ
QHYQPCLLCTLQCHLQEDL*TPSHVSL*EHRRY
KVKYL*KR*KVGKGSLRRIKGINELLVLKSLPL
HFIV*LQNVQFRSPAVTHLSRLGSSLQKLHLINC
QY*EQ*DNERNMLGSWI*ETIHCFS*SLEEGLL
ILQFYQSLHKRNNLVPFLLLLLLLFSCVLKRRK
CHSYKVNMET*T*RNRHYTMGT*KKKIKEGCRNC
LRSVKHILFFCYGPI*RIGM**MLIFCLSFSHH
EEKANKQKPQLGHTIFLLVMPLFCTSLFYIVYR
ENLQDFH*S*PETAMKMDVSPL

Sequence NM_000739

ACAGACTAGCCAAGTGGCTGAGACGAGTGTGGGGTGCGTGACTCTGCCTGCGCGCGCGCCAGCCCCGCAGCTCTCGCCAGAGCCTTGGGATCAAGGGAGA
AAGAGAACCGGCAGCTGGCCTCGGACTCTAAGCGGTGCGCAGCTCCAGCCCGAGCGGATCGGCCCTGAACCCACAAAGGACTCCTCGCTCCTTCAAGCCT
CCACCACCTCGCAGCCGGGGAGGCAACTGGAGCGAAACCAGCGACAGGTTGTTAGCGACATGAGTACAGGGTCTGGATCTTACCCATTCAAGAGTGTATT
CTCAGCACCCAATGCCTGGCATATAGTTTAAATGTTTATTTGCTACTTGGCTACTGATTAGAGAACGCAAAATGAATAACTCAACAAACTCCTCTAACAA
TAGCCTGGCTCTTACAAGTCCTTATAAGACATTTGAAGTGGTGTTTATTGTCCTGGTGGCTGGATCCCTCAGTTTGGTGACCATTATCGGGAACATCCTA
GTCATGGTTTCCATTAAAGTCAACCGCCACCTCCAGACCGTCAACAATTACTTTTTATTCAGCTTGGCCTGTGCTGACCTTATCATAGGTGTTTTCTCCA
TGAACTTGTACACCCTCTACACTGTGATTGGTTACTGGCCTTTGGGACCTGTGGTGTGTGACCTTTGGCTAGCCCTGGACTATGTGGTCAGCAATGCCTC
AGTTATGAATCTGCTCATCATCAGCTTTGACAGGTACTTCTGTGTCACAAAACCTCTGACCTACCCAGTCAAGCGGACCACAAAAATGGCAGGTATGATG
ATTGCAGCTGCCTGGGTCCTCTCTTTCATCCTCTGGGCTCCAGCCATTCTCTTCTGGCAGTTCATTGTAGGGGTGAGAACTGTGGAGGATGGGGAGTGCT
ACATTCAGTTTTTTTCCAATGCTGCTGTCACCTTTGGTACGGCTATTGCAGCCTTCTATTTGCCAGTGATCATCATGACTGTGCTATATTGGCACATATC
CCGAGCCAGCAAGAGCAGGATAAAGAAGGACAAGAAGGAGCCTGTTGCCAACCAAGACCCCGTTTCTCCAAGTCTGGTACAAGGAAGGATAGTGAAGCCA
AACAATAACAACATGCCCAGCAGTGACGATGGCCTGGAGCACAACAAAATCCAGAATGGCAAAGCCCCCAGGGATCCTGTGACTGAAAACTGTGTTCAGG
GAGAGGAGAAGGAGAGCTCCAATGACTCCACCTCAGTCAGTGCTGTTGCCTCTAATATGAGAGATGATGAAATAACCCAGGATGAAAACACAGTTTCCAC
TTCCCTGGGCCATTCCAAAGATGAGAACTCTAAGCAAACATGCATCAGAATTGGCACCAAGACCCCAAAAAGTGACTCATGTACCCCAACTAATACCACC
GTGGAGGTAGTGGGGTCTTCAGGTCAGAATGGAGATGAAAAGCAGAATATTGTAGCCCGCAAGATTGTGAAGATGACTAAGCAGCCTGCAAAAAAGAAGC
CTCCTCCTTCCCGGGAAAAGAAAGTCACCAGGACAATCTTGGCTATTCTGTTGGCTTTCATCATCACTTGGGCCCCATACAATGTCATGGTGCTCATTAA
CACCTTTTGTGCACCTTGCATCCCCAACACTGTGTGGACAATTGGTTACTGGCTTTGTTACATCAACAGCACTATCAACCCTGCCTGCTATGCACTTTGC
AATGCCACCTTCAAGAAGACCTTTAAACACCTTCTCATGTGTCATTATAAGAACATAGGCGCTACAAGGTAAAATATCTTTGAAAAAGATAGAAGGTGGG
CAAGGGGAGCTTGAGAAGAATAAAAGGGATAAACGAGCTCCTAGTTTTAAAATCTCTGCCATTGCACTTTATAGTCTGATTACAAAACGTGCAATTCAGG
AGCCCAGCAGTGACACACTTATCACGCCTAGGCTCCAGTTTGCAAAAATTGCACCTTATAAACTGTCAGTATTAGGAGCAATGAGACAATGAAAGAAACA
TGTTGGGATCGTGGATTTAAGAAACTATACACTGTTTCTCATAATCTCTTGAAGAAGGGCTTCTGATTCTACAATTTTATCAGTCTCTGCACAAGAGGAA
TAACCTTGTTCCTTTTTTGTTACTTTTGTTGTTGTTGTTCTCATGTGTCCTTAAGAGAAGGAAATGCCACAGTTACAAGGTAAACATGGAGACTTAAACA
TAAAGAAATAGGCACTATACAATGGGGACATAAAAAAAGAAAATCAAAGAAGGATGCAGAAATTGTCTCCGGAGTGTTAAGCATATTTTATTCTTTTGTT
ACGGTCCTATTTAGAGGATTGGAATGTAATAAATGCTTATTTTTTGCCTTTCTTTTTCCCACCATGAAGAGAAAGCAAACAAACAGAAACCCCAACTAGG
TCACACCATTTTTCTTCTTGTTATGCCACTATTTTGTACATCCCTGTTCTATATTGTTTATAGGGAAAACCTACAGGACTTTCACTAAAGCTAGCCAGAG
ACAGCCATGAAAATGGACGTTTCACCCCTC
  • refseq_CHRM2.F3 (forward primer)
  • refseq_CHRM2.R3 (reverse primer)
  • Single exon skipping

Frame 0

TD*PSG*DECGVRDSACARASPAALARALGSRE
KENRQLASDSKRCAAPARADRP*THKGLLAPSSL
HHLAAGEATGAKPATGC*RHEYRVWILPIQECI
LSTQCLAYSLNVYLLLGY*LENAK*ITQQTPLT
IAWLLQVLIRHLKWCLLSWWLDPSVW*PLSGTS*
SWFPLKSTATSRPSTITFYSAWPVLTLS*VFSP
*TCTPSTL*LVTGLWDLWCVTFG*PWTMWSAMP
QL*ICSSSALTGTSVSQNL*PTQSSGPQKWQV**
LQLPGSSLSSSGLQPFSSGSSL*G*ELWRMGSA
TFSFFPMLLSPLVRLLQPSICQ*SS*LCYIGTY
PEPARAG*RRTRRSLLPTKTPFLQVWYKEG**SQ
TITTCPAVTMAWSTTKSRMAKPPGIL*LKTVFR
ERRRRAPMTPPQSVLLPLI*EMMK*PRMKTQFP
LPWAIPKMRTLSKHASELAPRPQKVTHVPQLIPP
WR*WGLQVRMEMKSRIL*PARL*R*LSSLQKRS
LLLPGKRKSPGQSWLFCWLSSSLGPHTMSWCSL
TPFVHLASPTLCGQLVTGFVTSTALSTLPAMHFA
MPPSRRPLNTFSCVIIRT*ALQGKISLKKIEGG
QGELEKNKRDKRAPSFKISAIALYSLITKRAIQ
EPSSDTLITPRLQFAKIAPYKLSVLGAMRQ*KKH
VGIVDLRNYTLFLIIS*RRASDSTILSVSAQEE
*PCSFFVTFVVVVLMCP*EKEMPQLQGKHGDLN
IKK*ALYNGDIKKENQRRMQKLSPEC*AYFILLL
RSYLEDWNVINAYFLPFFFPP*RESKQTETPTR
SHHFSSCYATILYIPVLYCL*GKPTGLSLKLAR
DSHENGRFTP

Frame 1

QTSQVAETSVGCVTLPARAPAPQLSPEPWDQGR
KRTGSWPRTLSGAQLQPERIGPEPTKDSSLLQA
STTSQPGRQLERNQRQVVSDMSTGSGSYPFKSVF
SAPNAWHIV*MFICYLATD*RTQNE*LNKLL*Q
*PGSYKSL*DI*SGVYCPGGWIPQFGDHYREHP
SHGFH*SQPPPPDRQQLLFIQLGLC*PYHRCFLH
ELVHPLHCDWLLAFGTCGV*PLASPGLCGQQCL
SYESAHHQL*QVLLCHKTSDLPSQADHKNGRYD
DCSCLGPLFHPLGSSHSLLAVHCRGENCGGWGVL
HSVFFQCCCHLWYGYCSLLFASDHHDCAILAHI
PSQQEQDKEGQEGACCQPRPRFSKSGTRKDSEA
KQ*QHAQQ*RWPGAQQNPEWQSPQGSCD*KLCSG
RGEGELQ*LHLSQCCCL*YER**NNPG*KHSFH
FPGPFQR*EL*ANMHQNWHQDPKK*LMYPN*YH
RGGSGVFRSEWR*KAEYCSPQDCEDD*AACKKEA
SSFPGKESHQDNLGYSVGFHHHLGPIQCHGAH*
HLLCTLHPQHCVDNWLLALLHQQHYQPCLLCTL
QCHLQEDL*TPSHVSL*EHRRYKVKYL*KR*KVG
KGSLRRIKGINELLVLKSLPLHFIV*LQNVQFR
SPAVTHLSRLGSSLQKLHLINCQY*EQ*DNERN
MLGSWI*ETIHCFS*SLEEGLLILQFYQSLHKRN
NLVPFLLLLLLLFSCVLKRRKCHSYKVNMET*T
*RNRHYTMGT*KKKIKEGCRNCLRSVKHILFFC
YGPI*RIGM**MLIFCLSFSHHEEKANKQKPQLG
HTIFLLVMPLFCTSLFYIVYRENLQDFH*S*PE
TAMKMDVSPL

Frame 2

RLAKWLRRVWGA*LCLRARQPRSSRQSLGIKGE
REPAAGLGL*AVRSSSPSGSALNPQRTPRSFKP
PPPRSRGGNWSETSDRLLAT*VQGLDLTHSRVY
SQHPMPGI*FKCLFATWLLIRERKMNNSTNSSNN
SLALTSPYKTFEVVFIVLVAGSLSLVTIIGNIL
VMVSIKVNRHLQTVNNYFLFSLACADLIIGVFS
MNLYTLYTVIGYWPLGPVVCDLWLALDYVVSNAS
VMNLLIISFDRYFCVTKPLTYPVKRTTKMAGMM
IAAAWVLSFILWAPAILFWQFIVGVRTVEDGEC
YIQFFSNAAVTFGTAIAAFYLPVIIMTVLYWHIS
RASKSRIKKDKKEPVANQDPVSPSLVQGRIVKP
NNNNMPSSDDGLEHNKIQNGKAPRDPVTENCVQ
GEEKESSNDSTSVSAVASNMRDDEITQDENTVST
SLGHSKDENSKQTCIRIGTKTPKSDSCTPTNTT
VEVVGSSGQNGDEKQNIVARKIVKMTKQPAKKK
PPPSREKKVTRTILAILLAFIITWAPYNVMVLIN
TFCAPCIPNTVWTIGYWLCYINSTINPACYALC
NATFKKTFKHLLMCHYKNIGATR*NIFEKDRRW
ARGA*EE*KG*TSS*F*NLCHCTL*SDYKTCNSG
AQQ*HTYHA*APVCKNCTL*TVSIRSNETMKET
CWDRGFKKLYTVSHNLLKKGF*FYNFISLCTRG
ITLFLFCYFCCCCSHVSLREGNATVTR*TWRLKH
KEIGTIQWGHKKRKSKKDAEIVSGVLSIFYSFV
TVLFRGLECNKCLFFAFLFPTMKRKQTNRNPN*
VTPFFFLLCHYFVHPCSILFIGKTYRTFTKASQR
QP*KWTFHP

Sequence NM_001006631

ACAGACTAGCCAAGTGGCTGAGACGAGTGTGGGATCAAGGGAGAAAGAGAACCGGCAGCTGGCCTCGGACTCTAAGCGGTGCGCAGCTCCAGCCCGAGCG
GATCGGCCCTGAACCCACAAAGGACTCCTCGCTCCTTCAAGCCTCCACCACCTCGCAGCCGGGGAGGCAACTGGAGCGAAACCAGCGACAGGTTGTTAGC
GACATGAGTACAGGGTCTGGATCTTACCCATTCAAGAGTGTATTCTCAGCACCCAATGCCTGGCATATAGTTTAAATGTTTATTTGCTACTTGGCTACTG
ATTAGAGAACGCAAAATGAATAACTCAACAAACTCCTCTAACAATAGCCTGGCTCTTACAAGTCCTTATAAGACATTTGAAGTGGTGTTTATTGTCCTGG
TGGCTGGATCCCTCAGTTTGGTGACCATTATCGGGAACATCCTAGTCATGGTTTCCATTAAAGTCAACCGCCACCTCCAGACCGTCAACAATTACTTTTT
ATTCAGCTTGGCCTGTGCTGACCTTATCATAGGTGTTTTCTCCATGAACTTGTACACCCTCTACACTGTGATTGGTTACTGGCCTTTGGGACCTGTGGTG
TGTGACCTTTGGCTAGCCCTGGACTATGTGGTCAGCAATGCCTCAGTTATGAATCTGCTCATCATCAGCTTTGACAGGTACTTCTGTGTCACAAAACCTC
TGACCTACCCAGTCAAGCGGACCACAAAAATGGCAGGTATGATGATTGCAGCTGCCTGGGTCCTCTCTTTCATCCTCTGGGCTCCAGCCATTCTCTTCTG
GCAGTTCATTGTAGGGGTGAGAACTGTGGAGGATGGGGAGTGCTACATTCAGTTTTTTTCCAATGCTGCTGTCACCTTTGGTACGGCTATTGCAGCCTTC
TATTTGCCAGTGATCATCATGACTGTGCTATATTGGCACATATCCCGAGCCAGCAAGAGCAGGATAAAGAAGGACAAGAAGGAGCCTGTTGCCAACCAAG
ACCCCGTTTCTCCAAGTCTGGTACAAGGAAGGATAGTGAAGCCAAACAATAACAACATGCCCAGCAGTGACGATGGCCTGGAGCACAACAAAATCCAGAA
TGGCAAAGCCCCCAGGGATCCTGTGACTGAAAACTGTGTTCAGGGAGAGGAGAAGGAGAGCTCCAATGACTCCACCTCAGTCAGTGCTGTTGCCTCTAAT
ATGAGAGATGATGAAATAACCCAGGATGAAAACACAGTTTCCACTTCCCTGGGCCATTCCAAAGATGAGAACTCTAAGCAAACATGCATCAGAATTGGCA
CCAAGACCCCAAAAAGTGACTCATGTACCCCAACTAATACCACCGTGGAGGTAGTGGGGTCTTCAGGTCAGAATGGAGATGAAAAGCAGAATATTGTAGC
CCGCAAGATTGTGAAGATGACTAAGCAGCCTGCAAAAAAGAAGCCTCCTCCTTCCCGGGAAAAGAAAGTCACCAGGACAATCTTGGCTATTCTGTTGGCT
TTCATCATCACTTGGGCCCCATACAATGTCATGGTGCTCATTAACACCTTTTGTGCACCTTGCATCCCCAACACTGTGTGGACAATTGGTTACTGGCTTT
GTTACATCAACAGCACTATCAACCCTGCCTGCTATGCACTTTGCAATGCCACCTTCAAGAAGACCTTTAAACACCTTCTCATGTGTCATTATAAGAACAT
AGGCGCTACAAGGTAAAATATCTTTGAAAAAGATAGAAGGTGGGCAAGGGGAGCTTGAGAAGAATAAAAGGGATAAACGAGCTCCTAGTTTTAAAATCTC
TGCCATTGCACTTTATAGTCTGATTACAAAACGTGCAATTCAGGAGCCCAGCAGTGACACACTTATCACGCCTAGGCTCCAGTTTGCAAAAATTGCACCT
TATAAACTGTCAGTATTAGGAGCAATGAGACAATGAAAGAAACATGTTGGGATCGTGGATTTAAGAAACTATACACTGTTTCTCATAATCTCTTGAAGAA
GGGCTTCTGATTCTACAATTTTATCAGTCTCTGCACAAGAGGAATAACCTTGTTCCTTTTTTGTTACTTTTGTTGTTGTTGTTCTCATGTGTCCTTAAGA
GAAGGAAATGCCACAGTTACAAGGTAAACATGGAGACTTAAACATAAAGAAATAGGCACTATACAATGGGGACATAAAAAAAGAAAATCAAAGAAGGATG
CAGAAATTGTCTCCGGAGTGTTAAGCATATTTTATTCTTTTGTTACGGTCCTATTTAGAGGATTGGAATGTAATAAATGCTTATTTTTTGCCTTTCTTTT
TCCCACCATGAAGAGAAAGCAAACAAACAGAAACCCCAACTAGGTCACACCATTTTTCTTCTTGTTATGCCACTATTTTGTACATCCCTGTTCTATATTG
TTTATAGGGAAAACCTACAGGACTTTCACTAAAGCTAGCCAGAGACAGCCATGAAAATGGACGTTTCACCCCTC
  • refseq_CHRM2.F3 (forward primer)
  • refseq_CHRM2.R3 (reverse primer)
  • Single exon skipping

Frame 0

TD*PSG*DECGIKGEREPAAGLGL*AVRSSSPS
GSALNPQRTPRSFKPPPPRSRGGNWSETSDRLLA
T*VQGLDLTHSRVYSQHPMPGI*FKCLFATWLL
IRERKMNNSTNSSNNSLALTSPYKTFEVVFIVL
VAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFL
FSLACADLIIGVFSMNLYTLYTVIGYWPLGPVV
CDLWLALDYVVSNASVMNLLIISFDRYFCVTKP
LTYPVKRTTKMAGMMIAAAWVLSFILWAPAILFW
QFIVGVRTVEDGECYIQFFSNAAVTFGTAIAAF
YLPVIIMTVLYWHISRASKSRIKKDKKEPVANQ
DPVSPSLVQGRIVKPNNNNMPSSDDGLEHNKIQN
GKAPRDPVTENCVQGEEKESSNDSTSVSAVASN
MRDDEITQDENTVSTSLGHSKDENSKQTCIRIG
TKTPKSDSCTPTNTTVEVVGSSGQNGDEKQNIVA
RKIVKMTKQPAKKKPPPSREKKVTRTILAILLA
FIITWAPYNVMVLINTFCAPCIPNTVWTIGYWL
CYINSTINPACYALCNATFKKTFKHLLMCHYKNI
GATR*NIFEKDRRWARGA*EE*KG*TSS*F*NL
CHCTL*SDYKTCNSGAQQ*HTYHA*APVCKNCT
L*TVSIRSNETMKETCWDRGFKKLYTVSHNLLKK
GF*FYNFISLCTRGITLFLFCYFCCCCSHVSLR
EGNATVTR*TWRLKHKEIGTIQWGHKKRKSKKD
AEIVSGVLSIFYSFVTVLFRGLECNKCLFFAFLF
PTMKRKQTNRNPN*VTPFFFLLCHYFVHPCSIL
FIGKTYRTFTKASQRQP*KWTFHP

Frame 1

QTSQVAETSVGSREKENRQLASDSKRCAAPARA
DRP*THKGLLAPSSLHHLAAGEATGAKPATGC*
RHEYRVWILPIQECILSTQCLAYSLNVYLLLGY*
LENAK*ITQQTPLTIAWLLQVLIRHLKWCLLSW
WLDPSVW*PLSGTS*SWFPLKSTATSRPSTITF
YSAWPVLTLS*VFSP*TCTPSTL*LVTGLWDLWC
VTFG*PWTMWSAMPQL*ICSSSALTGTSVSQNL
*PTQSSGPQKWQV**LQLPGSSLSSSGLQPFSS
GSSL*G*ELWRMGSATFSFFPMLLSPLVRLLQPS
ICQ*SS*LCYIGTYPEPARAG*RRTRRSLLPTK
TPFLQVWYKEG**SQTITTCPAVTMAWSTTKSR
MAKPPGIL*LKTVFRERRRRAPMTPPQSVLLPLI
*EMMK*PRMKTQFPLPWAIPKMRTLSKHASELA
PRPQKVTHVPQLIPPWR*WGLQVRMEMKSRIL*
PARL*R*LSSLQKRSLLLPGKRKSPGQSWLFCWL
SSSLGPHTMSWCSLTPFVHLASPTLCGQLVTGF
VTSTALSTLPAMHFAMPPSRRPLNTFSCVIIRT
*ALQGKISLKKIEGGQGELEKNKRDKRAPSFKIS
AIALYSLITKRAIQEPSSDTLITPRLQFAKIAP
YKLSVLGAMRQ*KKHVGIVDLRNYTLFLIIS*R
RASDSTILSVSAQEE*PCSFFVTFVVVVLMCP*E
KEMPQLQGKHGDLNIKK*ALYNGDIKKENQRRM
QKLSPEC*AYFILLLRSYLEDWNVINAYFLPFF
FPP*RESKQTETPTRSHHFSSCYATILYIPVLYC
L*GKPTGLSLKLARDSHENGRFTP

Frame 2

RLAKWLRRVWDQGRKRTGSWPRTLSGAQLQPER
IGPEPTKDSSLLQASTTSQPGRQLERNQRQVVS
DMSTGSGSYPFKSVFSAPNAWHIV*MFICYLAT
D*RTQNE*LNKLL*Q*PGSYKSL*DI*SGVYCPG
GWIPQFGDHYREHPSHGFH*SQPPPPDRQQLLF
IQLGLC*PYHRCFLHELVHPLHCDWLLAFGTCG
V*PLASPGLCGQQCLSYESAHHQL*QVLLCHKTS
DLPSQADHKNGRYDDCSCLGPLFHPLGSSHSLL
AVHCRGENCGGWGVLHSVFFQCCCHLWYGYCSL
LFASDHHDCAILAHIPSQQEQDKEGQEGACCQPR
PRFSKSGTRKDSEAKQ*QHAQQ*RWPGAQQNPE
WQSPQGSCD*KLCSGRGEGELQ*LHLSQCCCL*
YER**NNPG*KHSFHFPGPFQR*EL*ANMHQNWH
QDPKK*LMYPN*YHRGGSGVFRSEWR*KAEYCS
PQDCEDD*AACKKEASSFPGKESHQDNLGYSVG
FHHHLGPIQCHGAH*HLLCTLHPQHCVDNWLLAL
LHQQHYQPCLLCTLQCHLQEDL*TPSHVSL*EH
RRYKVKYL*KR*KVGKGSLRRIKGINELLVLKS
LPLHFIV*LQNVQFRSPAVTHLSRLGSSLQKLHL
INCQY*EQ*DNERNMLGSWI*ETIHCFS*SLEE
GLLILQFYQSLHKRNNLVPFLLLLLLLFSCVLK
RRKCHSYKVNMET*T*RNRHYTMGT*KKKIKEGC
RNCLRSVKHILFFCYGPI*RIGM**MLIFCLSF
SHHEEKANKQKPQLGHTIFLLVMPLFCTSLFYI
VYRENLQDFH*S*PETAMKMDVSPL

Sequence NM_001006630

ATTGCAGCTAATCGAACACACACGCACGAATACAGACACGCTCTCCTGCTGTACTAAAGGCGCCAGGGCGCAAAGACCTAGGGAGCGCGCGCGGGCACAC
ACACACACACACACACACACACAGACACACACACACTCACACACTCCAGGCTGCGGGTTGGCCAGAGCCTGGAAATCGCAGATTTCACCAGGAAATCGCC
GAGGTACCCGAATAGGTGTTGCCTCCCAAAACTAATTCCTACTTCCCCAAGCACTTTGGTGAAGAAATGAATCCAGCCCAGCTCGCCGAGGCATCCAGGT
CTCCATTCTATTTTGGTCTCCAGGCTCTTCGTGAAAGCCAGATCAAGGGAGAAAGAGAACCGGCAGCTGGCCTCGGACTCTAAGCGGTGCGCAGCTCCAG
CCCGAGCGGATCGGCCCTGAACCCACAAAGGACTCCTCGCTCCTTCAAGCCTCCACCACCTCGCAGCCGGGGAGGCAACTGGAGCGAAACCAGCGACAGG
TTGTTAGCGACATGAGTACAGGGTCTGGATCTTACCCATTCAAGAGTGTATTCTCAGCACCCAATGCCTGGCATATAGTTTAAATGTTTATTTGCTACTT
GGCTACTGATTAGAGAACGCAAAATGAATAACTCAACAAACTCCTCTAACAATAGCCTGGCTCTTACAAGTCCTTATAAGACATTTGAAGTGGTGTTTAT
TGTCCTGGTGGCTGGATCCCTCAGTTTGGTGACCATTATCGGGAACATCCTAGTCATGGTTTCCATTAAAGTCAACCGCCACCTCCAGACCGTCAACAAT
TACTTTTTATTCAGCTTGGCCTGTGCTGACCTTATCATAGGTGTTTTCTCCATGAACTTGTACACCCTCTACACTGTGATTGGTTACTGGCCTTTGGGAC
CTGTGGTGTGTGACCTTTGGCTAGCCCTGGACTATGTGGTCAGCAATGCCTCAGTTATGAATCTGCTCATCATCAGCTTTGACAGGTACTTCTGTGTCAC
AAAACCTCTGACCTACCCAGTCAAGCGGACCACAAAAATGGCAGGTATGATGATTGCAGCTGCCTGGGTCCTCTCTTTCATCCTCTGGGCTCCAGCCATT
CTCTTCTGGCAGTTCATTGTAGGGGTGAGAACTGTGGAGGATGGGGAGTGCTACATTCAGTTTTTTTCCAATGCTGCTGTCACCTTTGGTACGGCTATTG
CAGCCTTCTATTTGCCAGTGATCATCATGACTGTGCTATATTGGCACATATCCCGAGCCAGCAAGAGCAGGATAAAGAAGGACAAGAAGGAGCCTGTTGC
CAACCAAGACCCCGTTTCTCCAAGTCTGGTACAAGGAAGGATAGTGAAGCCAAACAATAACAACATGCCCAGCAGTGACGATGGCCTGGAGCACAACAAA
ATCCAGAATGGCAAAGCCCCCAGGGATCCTGTGACTGAAAACTGTGTTCAGGGAGAGGAGAAGGAGAGCTCCAATGACTCCACCTCAGTCAGTGCTGTTG
CCTCTAATATGAGAGATGATGAAATAACCCAGGATGAAAACACAGTTTCCACTTCCCTGGGCCATTCCAAAGATGAGAACTCTAAGCAAACATGCATCAG
AATTGGCACCAAGACCCCAAAAAGTGACTCATGTACCCCAACTAATACCACCGTGGAGGTAGTGGGGTCTTCAGGTCAGAATGGAGATGAAAAGCAGAAT
ATTGTAGCCCGCAAGATTGTGAAGATGACTAAGCAGCCTGCAAAAAAGAAGCCTCCTCCTTCCCGGGAAAAGAAAGTCACCAGGACAATCTTGGCTATTC
TGTTGGCTTTCATCATCACTTGGGCCCCATACAATGTCATGGTGCTCATTAACACCTTTTGTGCACCTTGCATCCCCAACACTGTGTGGACAATTGGTTA
CTGGCTTTGTTACATCAACAGCACTATCAACCCTGCCTGCTATGCACTTTGCAATGCCACCTTCAAGAAGACCTTTAAACACCTTCTCATGTGTCATTAT
AAGAACATAGGCGCTACAAGGTAAAATATCTTTGAAAAAGATAGAAGGTGGGCAAGGGGAGCTTGAGAAGAATAAAAGGGATAAACGAGCTCCTAGTTTT
AAAATCTCTGCCATTGCACTTTATAGTCTGATTACAAAACGTGCAATTCAGGAGCCCAGCAGTGACACACTTATCACGCCTAGGCTCCAGTTTGCAAAAA
TTGCACCTTATAAACTGTCAGTATTAGGAGCAATGAGACAATGAAAGAAACATGTTGGGATCGTGGATTTAAGAAACTATACACTGTTTCTCATAATCTC
TTGAAGAAGGGCTTCTGATTCTACAATTTTATCAGTCTCTGCACAAGAGGAATAACCTTGTTCCTTTTTTGTTACTTTTGTTGTTGTTGTTCTCATGTGT
CCTTAAGAGAAGGAAATGCCACAGTTACAAGGTAAACATGGAGACTTAAACATAAAGAAATAGGCACTATACAATGGGGACATAAAAAAAGAAAATCAAA
GAAGGATGCAGAAATTGTCTCCGGAGTGTTAAGCATATTTTATTCTTTTGTTACGGTCCTATTTAGAGGATTGGAATGTAATAAATGCTTATTTTTTGCC
TTTCTTTTTCCCACCATGAAGAGAAAGCAAACAAACAGAAACCCCAACTAGGTCACACCATTTTTCTTCTTGTTATGCCACTATTTTGTACATCCCTGTT
CTATATTGTTTATAGGGAAAACCTACAGGACTTTCACTAAAGCTAGCCAGAGACAGCCATGAAAATGGACGTTTCACCCCTC
  • refseq_CHRM2.F3 (forward primer)
  • refseq_CHRM2.R3 (reverse primer)
  • Single exon skipping

Frame 0

IAANRTHTHEYRHALLLY*RRQGAKT*GARAGT
HTHTHTHTDTHTLTHSRLRVGQSLEIADFTRKSP
RYPNRCCLPKLIPTSPSTLVKK*IQPSSPRHPG
LHSILVSRLFVKARSREKENRQLASDSKRCAAP
ARADRP*THKGLLAPSSLHHLAAGEATGAKPATG
C*RHEYRVWILPIQECILSTQCLAYSLNVYLLL
GY*LENAK*ITQQTPLTIAWLLQVLIRHLKWCL
LSWWLDPSVW*PLSGTS*SWFPLKSTATSRPSTI
TFYSAWPVLTLS*VFSP*TCTPSTL*LVTGLWD
LWCVTFG*PWTMWSAMPQL*ICSSSALTGTSVS
QNL*PTQSSGPQKWQV**LQLPGSSLSSSGLQPF
SSGSSL*G*ELWRMGSATFSFFPMLLSPLVRLL
QPSICQ*SS*LCYIGTYPEPARAG*RRTRRSLL
PTKTPFLQVWYKEG**SQTITTCPAVTMAWSTTK
SRMAKPPGIL*LKTVFRERRRRAPMTPPQSVLL
PLI*EMMK*PRMKTQFPLPWAIPKMRTLSKHAS
ELAPRPQKVTHVPQLIPPWR*WGLQVRMEMKSRI
L*PARL*R*LSSLQKRSLLLPGKRKSPGQSWLF
CWLSSSLGPHTMSWCSLTPFVHLASPTLCGQLV
TGFVTSTALSTLPAMHFAMPPSRRPLNTFSCVII
RT*ALQGKISLKKIEGGQGELEKNKRDKRAPSF
KISAIALYSLITKRAIQEPSSDTLITPRLQFAK
IAPYKLSVLGAMRQ*KKHVGIVDLRNYTLFLIIS
*RRASDSTILSVSAQEE*PCSFFVTFVVVVLMC
P*EKEMPQLQGKHGDLNIKK*ALYNGDIKKENQ
RRMQKLSPEC*AYFILLLRSYLEDWNVINAYFLP
FFFPP*RESKQTETPTRSHHFSSCYATILYIPV
LYCL*GKPTGLSLKLARDSHENGRFTP

Frame 1

LQLIEHTRTNTDTLSCCTKGARAQRPRERARAH
THTHTHTQTHTHSHTPGCGLARAWKSQISPGNR
RGTRIGVASQN*FLLPQALW*RNESSPARRGIQV
SILFWSPGSS*KPDQGRKRTGSWPRTLSGAQLQ
PERIGPEPTKDSSLLQASTTSQPGRQLERNQRQ
VVSDMSTGSGSYPFKSVFSAPNAWHIV*MFICYL
ATD*RTQNE*LNKLL*Q*PGSYKSL*DI*SGVY
CPGGWIPQFGDHYREHPSHGFH*SQPPPPDRQQ
LLFIQLGLC*PYHRCFLHELVHPLHCDWLLAFGT
CGV*PLASPGLCGQQCLSYESAHHQL*QVLLCH
KTSDLPSQADHKNGRYDDCSCLGPLFHPLGSSH
SLLAVHCRGENCGGWGVLHSVFFQCCCHLWYGYC
SLLFASDHHDCAILAHIPSQQEQDKEGQEGACC
QPRPRFSKSGTRKDSEAKQ*QHAQQ*RWPGAQQ
NPEWQSPQGSCD*KLCSGRGEGELQ*LHLSQCCC
L*YER**NNPG*KHSFHFPGPFQR*EL*ANMHQ
NWHQDPKK*LMYPN*YHRGGSGVFRSEWR*KAE
YCSPQDCEDD*AACKKEASSFPGKESHQDNLGYS
VGFHHHLGPIQCHGAH*HLLCTLHPQHCVDNWL
LALLHQQHYQPCLLCTLQCHLQEDL*TPSHVSL
*EHRRYKVKYL*KR*KVGKGSLRRIKGINELLVL
KSLPLHFIV*LQNVQFRSPAVTHLSRLGSSLQK
LHLINCQY*EQ*DNERNMLGSWI*ETIHCFS*S
LEEGLLILQFYQSLHKRNNLVPFLLLLLLLFSCV
LKRRKCHSYKVNMET*T*RNRHYTMGT*KKKIK
EGCRNCLRSVKHILFFCYGPI*RIGM**MLIFC
LSFSHHEEKANKQKPQLGHTIFLLVMPLFCTSLF
YIVYRENLQDFH*S*PETAMKMDVSPL

Frame 2

CS*SNTHARIQTRSPAVLKAPGRKDLGSARGHT
HTHTHTHRHTHTHTLQAAGWPEPGNRRFHQEIA
EVPE*VLPPKTNSYFPKHFGEEMNPAQLAEASR
SPFYFGLQALRESQIKGEREPAAGLGL*AVRSSS
PSGSALNPQRTPRSFKPPPPRSRGGNWSETSDR
LLAT*VQGLDLTHSRVYSQHPMPGI*FKCLFAT
WLLIRERKMNNSTNSSNNSLALTSPYKTFEVVFI
VLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNN
YFLFSLACADLIIGVFSMNLYTLYTVIGYWPLG
PVVCDLWLALDYVVSNASVMNLLIISFDRYFCVT
KPLTYPVKRTTKMAGMMIAAAWVLSFILWAPAI
LFWQFIVGVRTVEDGECYIQFFSNAAVTFGTAI
AAFYLPVIIMTVLYWHISRASKSRIKKDKKEPVA
NQDPVSPSLVQGRIVKPNNNNMPSSDDGLEHNK
IQNGKAPRDPVTENCVQGEEKESSNDSTSVSAV
ASNMRDDEITQDENTVSTSLGHSKDENSKQTCIR
IGTKTPKSDSCTPTNTTVEVVGSSGQNGDEKQN
IVARKIVKMTKQPAKKKPPPSREKKVTRTILAI
LLAFIITWAPYNVMVLINTFCAPCIPNTVWTIGY
WLCYINSTINPACYALCNATFKKTFKHLLMCHY
KNIGATR*NIFEKDRRWARGA*EE*KG*TSS*F
*NLCHCTL*SDYKTCNSGAQQ*HTYHA*APVCKN
CTL*TVSIRSNETMKETCWDRGFKKLYTVSHNL
LKKGF*FYNFISLCTRGITLFLFCYFCCCCSHV
SLREGNATVTR*TWRLKHKEIGTIQWGHKKRKSK
KDAEIVSGVLSIFYSFVTVLFRGLECNKCLFFA
FLFPTMKRKQTNRNPN*VTPFFFLLCHYFVHPC
SILFIGKTYRTFTKASQRQP*KWTFHP

Sequence NM_001006626

ACAGACTAGCCAAGTGGCTGAGACGAGTGTGGGGTGCGTGACTCTGCCTGCGCGCGCGCCAGCCCCGCAGCTCTCGCCAGAGCCTTGGGATCAAGGGAGA
AAGAGAACCGGCAGCTGGCCTCGGACTCTAAGCGGTGCGCAGCTCCAGCCCGAGCGGATCGGCCCTGAACCCACAAAGGACTCCTCGCTCCTTCAAGCCT
CCACCACCTCGCAGCCGGGGAGGCAACTGGAGCGAAACCAGCGACAGGTTGTCCAGGCTGATCTTATACTCCTGGCCTCAAGTGATCCTCCTGCCTCAGT
CTCCAAAAGCACTGGGAATACATAGGTTGTTAGCGACATGAGTACAGGGTCTGGATCTTACCCATTCAAGAGTGTATTCTCAGCACCCAATGCCTGGCAT
ATAGTTTAAATGTTTATTTGCTACTTGGCTACTGATTAGAGAACGCAAAATGAATAACTCAACAAACTCCTCTAACAATAGCCTGGCTCTTACAAGTCCT
TATAAGACATTTGAAGTGGTGTTTATTGTCCTGGTGGCTGGATCCCTCAGTTTGGTGACCATTATCGGGAACATCCTAGTCATGGTTTCCATTAAAGTCA
ACCGCCACCTCCAGACCGTCAACAATTACTTTTTATTCAGCTTGGCCTGTGCTGACCTTATCATAGGTGTTTTCTCCATGAACTTGTACACCCTCTACAC
TGTGATTGGTTACTGGCCTTTGGGACCTGTGGTGTGTGACCTTTGGCTAGCCCTGGACTATGTGGTCAGCAATGCCTCAGTTATGAATCTGCTCATCATC
AGCTTTGACAGGTACTTCTGTGTCACAAAACCTCTGACCTACCCAGTCAAGCGGACCACAAAAATGGCAGGTATGATGATTGCAGCTGCCTGGGTCCTCT
CTTTCATCCTCTGGGCTCCAGCCATTCTCTTCTGGCAGTTCATTGTAGGGGTGAGAACTGTGGAGGATGGGGAGTGCTACATTCAGTTTTTTTCCAATGC
TGCTGTCACCTTTGGTACGGCTATTGCAGCCTTCTATTTGCCAGTGATCATCATGACTGTGCTATATTGGCACATATCCCGAGCCAGCAAGAGCAGGATA
AAGAAGGACAAGAAGGAGCCTGTTGCCAACCAAGACCCCGTTTCTCCAAGTCTGGTACAAGGAAGGATAGTGAAGCCAAACAATAACAACATGCCCAGCA
GTGACGATGGCCTGGAGCACAACAAAATCCAGAATGGCAAAGCCCCCAGGGATCCTGTGACTGAAAACTGTGTTCAGGGAGAGGAGAAGGAGAGCTCCAA
TGACTCCACCTCAGTCAGTGCTGTTGCCTCTAATATGAGAGATGATGAAATAACCCAGGATGAAAACACAGTTTCCACTTCCCTGGGCCATTCCAAAGAT
GAGAACTCTAAGCAAACATGCATCAGAATTGGCACCAAGACCCCAAAAAGTGACTCATGTACCCCAACTAATACCACCGTGGAGGTAGTGGGGTCTTCAG
GTCAGAATGGAGATGAAAAGCAGAATATTGTAGCCCGCAAGATTGTGAAGATGACTAAGCAGCCTGCAAAAAAGAAGCCTCCTCCTTCCCGGGAAAAGAA
AGTCACCAGGACAATCTTGGCTATTCTGTTGGCTTTCATCATCACTTGGGCCCCATACAATGTCATGGTGCTCATTAACACCTTTTGTGCACCTTGCATC
CCCAACACTGTGTGGACAATTGGTTACTGGCTTTGTTACATCAACAGCACTATCAACCCTGCCTGCTATGCACTTTGCAATGCCACCTTCAAGAAGACCT
TTAAACACCTTCTCATGTGTCATTATAAGAACATAGGCGCTACAAGGTAAAATATCTTTGAAAAAGATAGAAGGTGGGCAAGGGGAGCTTGAGAAGAATA
AAAGGGATAAACGAGCTCCTAGTTTTAAAATCTCTGCCATTGCACTTTATAGTCTGATTACAAAACGTGCAATTCAGGAGCCCAGCAGTGACACACTTAT
CACGCCTAGGCTCCAGTTTGCAAAAATTGCACCTTATAAACTGTCAGTATTAGGAGCAATGAGACAATGAAAGAAACATGTTGGGATCGTGGATTTAAGA
AACTATACACTGTTTCTCATAATCTCTTGAAGAAGGGCTTCTGATTCTACAATTTTATCAGTCTCTGCACAAGAGGAATAACCTTGTTCCTTTTTTGTTA
CTTTTGTTGTTGTTGTTCTCATGTGTCCTTAAGAGAAGGAAATGCCACAGTTACAAGGTAAACATGGAGACTTAAACATAAAGAAATAGGCACTATACAA
TGGGGACATAAAAAAAGAAAATCAAAGAAGGATGCAGAAATTGTCTCCGGAGTGTTAAGCATATTTTATTCTTTTGTTACGGTCCTATTTAGAGGATTGG
AATGTAATAAATGCTTATTTTTTGCCTTTCTTTTTCCCACCATGAAGAGAAAGCAAACAAACAGAAACCCCAACTAGGTCACACCATTTTTCTTCTTGTT
ATGCCACTATTTTGTACATCCCTGTTCTATATTGTTTATAGGGAAAACCTACAGGACTTTCACTAAAGCTAGCCAGAGACAGCCATGAAAATGGACGTTT
CACCCCTC
  • refseq_CHRM2.F3 (forward primer)
  • refseq_CHRM2.R3 (reverse primer)
  • Single exon skipping

Frame 0

TD*PSG*DECGVRDSACARASPAALARALGSRE
KENRQLASDSKRCAAPARADRP*THKGLLAPSSL
HHLAAGEATGAKPATGCPG*SYTPGLK*SSCLS
LQKHWEYIGC*RHEYRVWILPIQECILSTQCLA
YSLNVYLLLGY*LENAK*ITQQTPLTIAWLLQVL
IRHLKWCLLSWWLDPSVW*PLSGTS*SWFPLKS
TATSRPSTITFYSAWPVLTLS*VFSP*TCTPST
L*LVTGLWDLWCVTFG*PWTMWSAMPQL*ICSSS
ALTGTSVSQNL*PTQSSGPQKWQV**LQLPGSS
LSSSGLQPFSSGSSL*G*ELWRMGSATFSFFPM
LLSPLVRLLQPSICQ*SS*LCYIGTYPEPARAG*
RRTRRSLLPTKTPFLQVWYKEG**SQTITTCPA
VTMAWSTTKSRMAKPPGIL*LKTVFRERRRRAP
MTPPQSVLLPLI*EMMK*PRMKTQFPLPWAIPKM
RTLSKHASELAPRPQKVTHVPQLIPPWR*WGLQ
VRMEMKSRIL*PARL*R*LSSLQKRSLLLPGKR
KSPGQSWLFCWLSSSLGPHTMSWCSLTPFVHLAS
PTLCGQLVTGFVTSTALSTLPAMHFAMPPSRRP
LNTFSCVIIRT*ALQGKISLKKIEGGQGELEKN
KRDKRAPSFKISAIALYSLITKRAIQEPSSDTLI
TPRLQFAKIAPYKLSVLGAMRQ*KKHVGIVDLR
NYTLFLIIS*RRASDSTILSVSAQEE*PCSFFV
TFVVVVLMCP*EKEMPQLQGKHGDLNIKK*ALYN
GDIKKENQRRMQKLSPEC*AYFILLLRSYLEDW
NVINAYFLPFFFPP*RESKQTETPTRSHHFSSC
YATILYIPVLYCL*GKPTGLSLKLARDSHENGRF
TP

Frame 1

QTSQVAETSVGCVTLPARAPAPQLSPEPWDQGR
KRTGSWPRTLSGAQLQPERIGPEPTKDSSLLQA
STTSQPGRQLERNQRQVVQADLILLASSDPPASV
SKSTGNT*VVSDMSTGSGSYPFKSVFSAPNAWH
IV*MFICYLATD*RTQNE*LNKLL*Q*PGSYKS
L*DI*SGVYCPGGWIPQFGDHYREHPSHGFH*SQ
PPPPDRQQLLFIQLGLC*PYHRCFLHELVHPLH
CDWLLAFGTCGV*PLASPGLCGQQCLSYESAHH
QL*QVLLCHKTSDLPSQADHKNGRYDDCSCLGPL
FHPLGSSHSLLAVHCRGENCGGWGVLHSVFFQC
CCHLWYGYCSLLFASDHHDCAILAHIPSQQEQD
KEGQEGACCQPRPRFSKSGTRKDSEAKQ*QHAQQ
*RWPGAQQNPEWQSPQGSCD*KLCSGRGEGELQ
*LHLSQCCCL*YER**NNPG*KHSFHFPGPFQR
*EL*ANMHQNWHQDPKK*LMYPN*YHRGGSGVFR
SEWR*KAEYCSPQDCEDD*AACKKEASSFPGKE
SHQDNLGYSVGFHHHLGPIQCHGAH*HLLCTLH
PQHCVDNWLLALLHQQHYQPCLLCTLQCHLQEDL
*TPSHVSL*EHRRYKVKYL*KR*KVGKGSLRRI
KGINELLVLKSLPLHFIV*LQNVQFRSPAVTHL
SRLGSSLQKLHLINCQY*EQ*DNERNMLGSWI*E
TIHCFS*SLEEGLLILQFYQSLHKRNNLVPFLL
LLLLLFSCVLKRRKCHSYKVNMET*T*RNRHYT
MGT*KKKIKEGCRNCLRSVKHILFFCYGPI*RIG
M**MLIFCLSFSHHEEKANKQKPQLGHTIFLLV
MPLFCTSLFYIVYRENLQDFH*S*PETAMKMDV
SPL

Frame 2

RLAKWLRRVWGA*LCLRARQPRSSRQSLGIKGE
REPAAGLGL*AVRSSSPSGSALNPQRTPRSFKP
PPPRSRGGNWSETSDRLSRLILYSWPQVILLPQ
SPKALGIHRLLAT*VQGLDLTHSRVYSQHPMPGI
*FKCLFATWLLIRERKMNNSTNSSNNSLALTSP
YKTFEVVFIVLVAGSLSLVTIIGNILVMVSIKV
NRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYT
VIGYWPLGPVVCDLWLALDYVVSNASVMNLLII
SFDRYFCVTKPLTYPVKRTTKMAGMMIAAAWVL
SFILWAPAILFWQFIVGVRTVEDGECYIQFFSNA
AVTFGTAIAAFYLPVIIMTVLYWHISRASKSRI
KKDKKEPVANQDPVSPSLVQGRIVKPNNNNMPS
SDDGLEHNKIQNGKAPRDPVTENCVQGEEKESSN
DSTSVSAVASNMRDDEITQDENTVSTSLGHSKD
ENSKQTCIRIGTKTPKSDSCTPTNTTVEVVGSS
GQNGDEKQNIVARKIVKMTKQPAKKKPPPSREKK
VTRTILAILLAFIITWAPYNVMVLINTFCAPCI
PNTVWTIGYWLCYINSTINPACYALCNATFKKT
FKHLLMCHYKNIGATR*NIFEKDRRWARGA*EE*
KG*TSS*F*NLCHCTL*SDYKTCNSGAQQ*HTY
HA*APVCKNCTL*TVSIRSNETMKETCWDRGFK
KLYTVSHNLLKKGF*FYNFISLCTRGITLFLFCY
FCCCCSHVSLREGNATVTR*TWRLKHKEIGTIQ
WGHKKRKSKKDAEIVSGVLSIFYSFVTVLFRGL
ECNKCLFFAFLFPTMKRKQTNRNPN*VTPFFFLL
CHYFVHPCSILFIGKTYRTFTKASQRQP*KWTF
HP

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