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Sequence NM_007087
AGCGCGAGTGCCAGAGCCCAGCCGGCGCGGAGCGGGAGCGGTGCAGGCTGAGGTCTCCGAGCGGCTCGCCATGGCTGGCCCGCAGCAGCAGCCCCCTTACCTGCACCTGGCCGAGCTGACGGCGTCCCAGTTCCTGGAAATATGGAAGCACTTTGACGCAGACGGAAATGGGTATATTGAAGGTAAAGAGCTAGAAAACT
TTTTCCAAGAGCTGGAGAAGGCAAGGAAAGGCTCTGGCATGATGTCAAAGAGTGACAACTTTGGAGAAAAGATGAAGGAGTTCATGCAGAAGTATGATAA
AAACTCAGATGGGAAAATCGAGATGGCAGAGCTGGCGCAGATCCTGCCAACCGAAGAGAACTTCCTTCTGTGCTTCAGGCAGCACGTGGGCTCCAGCGCC
GAGTTTATGGAGGCTTGGCGGAAGTACGACACAGACAGGAGTGGCTACATCGAAGCCAATGAGCTCAAGGGATTCCTGTCAGACCTGCTGAAGAAGGCGA
ACCGGCCGTACGATGAGCCCAAGCTCCAGGAATACACCCAAACCATACTACGGATGTTTGACTTGAACGGGGATGGCAAATTGGGCCTCTCAGAGATGTC
CCGGGCATGAAGCTGACCTCAGAGGAGTTTAACGCGATCTTCACATTTTACGACAAGGATAGAAGCGGCTACATTGACGAGCATGAGCTGGATGCCCTTT
TGAAGGATCTGTACGAGAAAAACAAAAAGGAAATGAATATTCAACAGCTCACCAACTACAGAAAGAGCGTCATGTCCTTGGCAGAGGCAGGGAAGCTCTA
CCGCAAGGACCTGGAGATTGTGCTCTGCAGCGAGCCCCCCATGTAAAGTGGGGACGGGGGCTGCTTCTCCACCTCCCCCAAACCCTGCTTCTGCTGCCCT
GATGCGTCTACCCAGACTCAGAGACCGTGAGCGCCCCGCCCCCACCCCTACAGCCTGCACACACCTGCCTGCAGAGCAGGAAATGAGAGATAGAGGATGG
GCAGCTGGGGGGCTGTCCTGAGCCCCCTGCACCCACCCCTGCCCAGGCAGTCTTTGCTCAGTGGATCACACACATGGAAGGTGATGGGGGCATGGGTGGA
GGGTCCCTAATTCTCTTCGCTGTGATGCATGAGCTCCCTCGCTGTATGATTTAGGCTTCTATGTCCAACAGAGTGGACTCTTCCCTCTCGCTCCCCTCTG
CCGGTCCCCCATGCCACCACCCACCCCAAACTTCCAGGTTCCATCCACCACCTTGCCAATGGTGTAGCTGTCCTCTCAGAACTCCTGTGTGTGGAAGGCA
CCCGCCCTTTCCTTGCCTTCTTTACTCGGCGTGCTCCTTTTCTCTTTGGGTTTCTTGTTTACCAAAGAAGAGTTTACAGACAATAAAATGGAAAGGTCCT
GCTGTGGAAACTT
- refseq_CALB2.F2 (forward primer)
- refseq_CALB2.R2 (reverse primer)
- Single exon skipping
Frame 0
SASARAQPARSGSGAG*GLRAARHGWPAAAAPLPAPGRADGVPVPGNMEAL*RRRKWVY*R*RARKL
FPRAGEGKERLWHDVKE*QLWRKDEGVHAEV**
KLRWENRDGRAGADPANRRELPSVLQAARGLQR
RVYGGLAEVRHRQEWLHRSQ*AQGIPVRPAEEGE
PAVR*AQAPGIHPNHTTDV*LERGWQIGPLRDV
PGMKLTSEEFNAIFTFYDKDRSGYIDEHELDAL
LKDLYEKNKKEMNIQQLTNYRKSVMSLAEAGKLY
RKDLEIVLCSEPPM*SGDGGCFSTSPKPCFCCP
DASTQTQRP*APRPHPYSLHTPACRAGNER*RM
GSWGAVLSPLHPPLPRQSLLSGSHTWKVMGAWVE
GP*FSSL*CMSSLAV*FRLLCPTEWTLPSRSPL
PVPHATTHPKLPGSIHHLANGVAVLSELLCVEG
TRPFLAFFTRRAPFLFGFLVYQRRVYRQ*NGKVL
LWKL
Frame 1
ARVPEPSRRGAGAVQAEVSERLAMAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELEN
FFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDK
NSDGKIEMAELAQILPTEENFLLCFRQHVGSSA
EFMEAWRKYDTDRSGYIEANELKGFLSDLLKKA
NRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMS
RA*S*PQRSLTRSSHFTTRIEAATLTSMSWMPF
*RICTRKTKRK*IFNSSPTTERASCPWQRQGSS
TARTWRLCSAASPPCKVGTGAASPPPPNPASAAL
MRLPRLRDRERPAPTPTACTHLPAEQEMRDRGW
AAGGLS*APCTHPCPGSLCSVDHTHGR*WGHGW
RVPNSLRCDA*APSLYDLGFYVQQSGLFPLAPLC
RSPMPPPTPNFQVPSTTLPMV*LSSQNSCVWKA
PALSLPSLLGVLLFSLGFLFTKEEFTDNKMERS
CCGN
Frame 2
RECQSPAGAERERCRLRSPSGSPWLARSSSPLTCTWPS*RRPSSWKYGSTLTQTEMGILKVKS*KT
FSKSWRRQGKALA*CQRVTTLEKR*RSSCRSMI
KTQMGKSRWQSWRRSCQPKRTSFCASGSTWAPAP
SLWRLGGSTTQTGVATSKPMSSRDSCQTC*RRR
TGRTMSPSSRNTPKPYYGCLT*TGMANWASQRC
PGHEADLRGV*RDLHILRQG*KRLH*RA*AGCPF
EGSVREKQKGNEYSTAHQLQKERHVLGRGREAL
PQGPGDCALQRAPHVKWGRGLLLHLPQTLLLLP
*CVYPDSETVSAPPPPLQPAHTCLQSRK*EIEDG
QLGGCPEPPAPTPAQAVFAQWITHMEGDGGMGG
GSLILFAVMHELPRCMI*ASMSNRVDSSLSLPS
AGPPCHHPPQTSRFHPPPCQWCSCPLRTPVCGRH
PPFPCLLYSACSFSLWVSCLPKKSLQTIKWKGP
AVET
Sequence NM_001740
AGCGCGAGTGCCAGAGCCCAGCCGGCGCGGAGCGGGAGCGGTGCAGGCTGAGGTCTCCGAGCGGCTCGCCATGGCTGGCCCGCAGCAGCAGCCCCCTTACCTGCACCTGGCCGAGCTGACGGCGTCCCAGTTCCTGGAAATATGGAAGCACTTTGACGCAGACGGAAATGGGTATATTGAAGGTAAAGAGCTAGAAAACT
TTTTCCAAGAGCTGGAGAAGGCAAGGAAAGGCTCTGGCATGATGTCAAAGAGTGACAACTTTGGAGAAAAGATGAAGGAGTTCATGCAGAAGTATGATAA
AAACTCAGATGGGAAAATCGAGATGGCAGAGCTGGCGCAGATCCTGCCAACCGAAGAGAACTTCCTTCTGTGCTTCAGGCAGCACGTGGGCTCCAGCGCC
GAGTTTATGGAGGCTTGGCGGAAGTACGACACAGACAGGAGTGGCTACATCGAAGCCAATGAGCTCAAGGGATTCCTGTCAGACCTGCTGAAGAAGGCGA
ACCGGCCGTACGATGAGCCCAAGCTCCAGGAATACACCCAAACCATACTACGGATGTTTGACTTGAACGGGGATGGCAAATTGGGCCTCTCAGAGATGTC
CCGACTCCTGCCTGTCCAGGAAAACTTCCTGCTTAAATTTCAGGGCATGAAGCTGACCTCAGAGGAGTTTAACGCGATCTTCACATTTTACGACAAGGAT
AGAAGCGGCTACATTGACGAGCATGAGCTGGATGCCCTTTTGAAGGATCTGTACGAGAAAAACAAAAAGGAAATGAATATTCAACAGCTCACCAACTACA
GAAAGAGCGTCATGTCCTTGGCAGAGGCAGGGAAGCTCTACCGCAAGGACCTGGAGATTGTGCTCTGCAGCGAGCCCCCCATGTAAAGTGGGGACGGGGG
CTGCTTCTCCACCTCCCCCAAACCCTGCTTCTGCTGCCCTGATGCGTCTACCCAGACTCAGAGACCGTGAGCGCCCCGCCCCCACCCCTACAGCCTGCAC
ACACCTGCCTGCAGAGCAGGAAATGAGAGATAGAGGATGGGCAGCTGGGGGGCTGTCCTGAGCCCCCTGCACCCACCCCTGCCCAGGCAGTCTTTGCTCA
GTGGATCACACACATGGAAGGTGATGGGGGCATGGGTGGAGGGTCCCTAATTCTCTTCGCTGTGATGCATGAGCTCCCTCGCTGTATGATTTAGGCTTCT
ATGTCCAACAGAGTGGACTCTTCCCTCTCGCTCCCCTCTGCCGGTCCCCCATGCCACCACCCACCCCAAACTTCCAGGTTCCATCCACCACCTTGCCAAT
GGTGTAGCTGTCCTCTCAGAACTCCTGTGTGTGGAAGGCACCCGCCCTTTCCTTGCCTTCTTTACTCGGCGTGCTCCTTTTCTCTTTGGGTTTCTTGTTT
ACCAAAGAAGAGTTTACAGACAATAAAATGGAAAGGTCCTGCTGTGGAAACTT
- refseq_CALB2.F2 (forward primer)
- refseq_CALB2.R2 (reverse primer)
- Single exon skipping
Frame 0
SASARAQPARSGSGAG*GLRAARHGWPAAAAPLPAPGRADGVPVPGNMEAL*RRRKWVY*R*RARKL
FPRAGEGKERLWHDVKE*QLWRKDEGVHAEV**
KLRWENRDGRAGADPANRRELPSVLQAARGLQR
RVYGGLAEVRHRQEWLHRSQ*AQGIPVRPAEEGE
PAVR*AQAPGIHPNHTTDV*LERGWQIGPLRDV
PTPACPGKLPA*ISGHEADLRGV*RDLHILRQG
*KRLH*RA*AGCPFEGSVREKQKGNEYSTAHQLQ
KERHVLGRGREALPQGPGDCALQRAPHVKWGRG
LLLHLPQTLLLLP*CVYPDSETVSAPPPPLQPA
HTCLQSRK*EIEDGQLGGCPEPPAPTPAQAVFAQ
WITHMEGDGGMGGGSLILFAVMHELPRCMI*AS
MSNRVDSSLSLPSAGPPCHHPPQTSRFHPPPCQ
WCSCPLRTPVCGRHPPFPCLLYSACSFSLWVSCL
PKKSLQTIKWKGPAVET
Frame 1
ARVPEPSRRGAGAVQAEVSERLAMAGPQQQPPYLHLAELTASQFLEIWKHFDADGNGYIEGKELEN
FFQELEKARKGSGMMSKSDNFGEKMKEFMQKYDK
NSDGKIEMAELAQILPTEENFLLCFRQHVGSSA
EFMEAWRKYDTDRSGYIEANELKGFLSDLLKKA
NRPYDEPKLQEYTQTILRMFDLNGDGKLGLSEMS
RLLPVQENFLLKFQGMKLTSEEFNAIFTFYDKD
RSGYIDEHELDALLKDLYEKNKKEMNIQQLTNY
RKSVMSLAEAGKLYRKDLEIVLCSEPPM*SGDGG
CFSTSPKPCFCCPDASTQTQRP*APRPHPYSLH
TPACRAGNER*RMGSWGAVLSPLHPPLPRQSLL
SGSHTWKVMGAWVEGP*FSSL*CMSSLAV*FRLL
CPTEWTLPSRSPLPVPHATTHPKLPGSIHHLAN
GVAVLSELLCVEGTRPFLAFFTRRAPFLFGFLV
YQRRVYRQ*NGKVLLWKL
Frame 2
RECQSPAGAERERCRLRSPSGSPWLARSSSPLTCTWPS*RRPSSWKYGSTLTQTEMGILKVKS*KT
FSKSWRRQGKALA*CQRVTTLEKR*RSSCRSMI
KTQMGKSRWQSWRRSCQPKRTSFCASGSTWAPAP
SLWRLGGSTTQTGVATSKPMSSRDSCQTC*RRR
TGRTMSPSSRNTPKPYYGCLT*TGMANWASQRC
PDSCLSRKTSCLNFRA*S*PQRSLTRSSHFTTRI
EAATLTSMSWMPF*RICTRKTKRK*IFNSSPTT
ERASCPWQRQGSSTARTWRLCSAASPPCKVGTG
AASPPPPNPASAALMRLPRLRDRERPAPTPTACT
HLPAEQEMRDRGWAAGGLS*APCTHPCPGSLCS
VDHTHGR*WGHGWRVPNSLRCDA*APSLYDLGF
YVQQSGLFPLAPLCRSPMPPPTPNFQVPSTTLPM
V*LSSQNSCVWKAPALSLPSLLGVLLFSLGFLF
TKEEFTDNKMERSCCGN
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