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Sequences

Sequence NM_004634

CGTCGCCGCCGCTGCTGCCGCCGCCGCTGCCACAGCCTTTGCCGCCACGGTCTCCGCCGCCGCTGTCCTCGCGCTGCCTCTGGGAGCCTCCATTGTTCCA
GCAGCGCCCGGTCCCGAGACCGGGCATCTTGGGGGCTCGCGAAACGGGCGGCGGCCGACGGAGTTGAGACCCCAGGGCGCCGGCGGGACCAGGAGCGGCA
GGGGTCAGCTCAGGAATCCCGGGGAGCCGGGACCAAAGCGTGGGGCGGCAGGGGGCCGGGGTGGCGAGGTCTACGGTCTCCGGAGTTGGGGCTCCCCCTT
CCCGGCCCGACTTTCCCCGCCGACCTCCCCAAGTGGGGTCGGAGCCCCGGCGGGCGCCCCCGGCGATGAGCCCGGACTCGAGGTGGCCGATGTGACAGCA
TGGGGGTGGACTTTGATGTGAAGACTTTCTGCCACAACTTGCGGGCGACTAAGCCACCATACGAGTGCCCGGTGGAGACCTGCCGAAAGGTCTACAAGAG
TTACAGTGGTATTGAGTACCACCTGTACCACTATGACCACGACAACCCACCACCCCCACAACAAACTCCACTCCGCAAGCACAAGAAAAAGGGGCGCCAG
TCACGCCCAGCCAACAAGCAGTCACCCAGCCCCTCAGAGGTCTCACAGTCACCAGGCCGTGAGGTGATGAGCTATGCACAGGCCCAGCGCATGGTGGAGG
TGGACTTGCATGGCCGCGTCCACCGCATCAGCATCTTTGACAACCTGGATGTGGTGTCAGAGGATGAGGAAGCCCCCGAGGAGGCCCCTGAGAATGGCAG
CAACAAGGAGAACACTGAGACACCAGCTGCTACTCCCAAGTCAGGCAAACATAAGAACAAGGAGAAGCGCAAGGACTCCAACCATCACCACCACCACAAT
GTTTCTGCGAGCACCACTCCCAAGCTGCCAGAGGTGGTCTATCGGGAGCTGGAACAGGACACCCCTGATGCCCCACCCCGGCCAACTTCCTATTACCGGT
ACATCGAGAAGTCTGCAGAGGAGCTGGACGAGGAAGTAGAGTATGACATGGACGAGGAGGACTACATCTGGCTGGATATCATGAATGAGCGTCGGAAGAC
AGAGGGTGTAAGTCCCATCCCGCAGGAGATCTTTGAGTACCTAATGGACCGACTGGAGAAGGAGTCGTACTTTGAGAGTCATAATAAAGGCGACCCTAAT
GCGCTAGTGGACGAGGATGCTGTTTGCTGTATCTGCAATGATGGTGAGTGCCAGAACAGCAATGTCATCCTCTTCTGTGACATGTGCAACCTGGCCGTGC
ACCAGGAGTGCTACGGTGTCCCCTATATCCCTGAGGGCCAGTGGCTGTGCCGCCGTTGCCTGCAGTCACCCTCTCGTGCTGTGGATTGTGCCCTGTGCCC
CAACAAGGGCGGTGCCTTCAAGCAGACAGATGACGGGCGCTGGGCCCATGTGGTGTGTGCCTTGTGGATCCCTGAGGTCTGCTTCGCCAACACGGTCTTC
CTAGAGCCTATTGACAGCATTGAGCACATCCCACCAGCTCGCTGGAAGCTCACCTGCTACATTTGCAAACAACGGGGCTCAGGGGCCTGCATCCAGTGCC
ACAAGGCCAACTGTTACACAGCTTTCCATGTGACATGCGCCCAGCAGGCTGGCCTTTACATGAAGATGGAGCCTGTGCGGGAGACAGGCGCCAACGGCAC
CTCTTTCAGTGTCCGCAAGACAGCCTACTGCGACATCCACACGCCTCCAGGTTCAGCACGCCGACTGCCTGCCCTGTCCCACAGCGAGGGTGAGGAGGAT
GAAGATGAGGAGGAGGATGAGGGTAAGGGCTGGAGCTCAGAGAAAGTCAAGAAGGCCAAGGCCAAGTCCCGGATCAAAATGAAGAAGGCACGGAAGATCC
TGGCAGAGAAGCGGGCAGCAGCACCTGTGGTGTCAGTGCCCTGCATCCCACCACACAGGCTTAGTAAAATCACCAACCGCCTGACCATCCAAAGGAAGAG
CCAGTTCATGCAGAGGCTGCACAGCTACTGGACACTGAAGCGGCAGTCACGGAATGGGGTCCCATTGCTACGTCGCCTGCAGACACACCTGCAATCTCAG
AGGAACTGTGACCAAGTTGGGAGAGATTCTGAGGATAAGAACTGGGCCCTTAAAGAACAGCTCAAGTCCTGGCAGCGGCTCCGGCATGACTTGGAGCGAG
CTCGGCTGCTCGTGGAATTGATCCGCAAGCGGGAAAAACTCAAAAGGGAGACGATCAAGGTTCAGCAGATTGCCATGGAGATGCAGCTGACTCCTTTCCT
CATCCTCCTTCGCAAAACCTTGGAGCAGCTCCAAGAGAAGGACACAGGCAACATCTTCAGCGAGCCGGTCCCTCTGTCTGAGGTACCTGACTACCTAGAC
CACATCAAAAAGCCCATGGACTTTTTCACCATGAAGCAGAACTTGGAGGCTTACCGCTACCTGAATTTTGATGATTTTGAGGAGGACTTCAACCTCATCG
TCAGCAACTGCCTCAAGTATAACGCCAAGGACACCATCTTCTACCGGGCAGCAGTGCGGCTTCGTGAGCAGGGTGGTGCTGTGCTCCGCCAGGCCCGGCG
CCAGGCAGAAAAAATGGGCATTGACTTTGAGACGGGCATGCATATCCCCCACAGCCTGGCTGGAGATGAGGCCACACACCACACTGAAGATGCAGCCGAG
GAAGAGCGGCTGGTCTTGCTGGAGAACCAGAAGCACCTGCCAGTGGAAGAACAGCTAAAGCTGCTTCTGGAGCGGCTGGACGAAGTGAATGCCAGCAAGC
AGAGTGTGGGCCGCTCACGGCGTGCAAAGATGATCAAGAAAGAGATGACGGCACTGCGGCGGAAGCTTGCCCATCAGCGAGAGACGGGACGTGATGGCCC
TGAGCGGCATGGCCCCTCGAGCCGGGGTAGTCTGACACCCCACCCGGCAGCCTGTGACAAGGATGGGCAGACAGATAGTGCGGCAGAGGAGAGCAGCAGC
CAGGAGACAAGCAAAGGCCTGGGTCCCAACATGTCCTCAACCCCCGCACATGAGGTGGGCAGGAGAACCTCAGTTCTGTTCTCCAAAAAGAACCCGAAGA
CAGCTGGACCGCCCAAGAGGCCGGGCCGGCCCCCCAAAAACCGGGAGAGCCAGATGACCCCCAGCCACGGAGGCAGTCCTGTGGGGCCCCCCCAGCTCCC
CATCATGAGTTCCCTGCGTCAGCGCAAGCGGGGTAGGAGCCCCCGGCCCAGTTCGAGCTCAGACAGCGACAGTGATAAGTCCACAGAAGACCCCCCAATG
GACTTACCAGCCAATGGCTTCAGCGGTGGAAACCAACCAGTGAAGAAGAGTTTCTTGGTATACCGTAATGACTGCAGCCTTCCCCGGAGCAGCTCAGACT
CTGAGTCCAGCAGCAGTAGCAGTAGCAGCGCTGCTTCAGACCGGACCAGCACAACGCCCTCAAAACAAGGCCGGGGCAAACCCTCCTTCTCTCGGGGCAC
TTTCCCAGAGGACAGCAGTGAGGATACCTCAGGCACTGAGAATGAGGCCTACTCCGTGGGCACTGGCCGCGGCGTGGGCCACAGCATGGTAAGGAAGAGT
CTGGGCCGGGGAGCTGGCTGGCTGTCAGAGGATGAGGACTCCCCGCTGGATGCTCTGGACCTCGTGTGGGCCAAATGCCGAGGCTATCCATCATACCCAG
CTCTGATCATTGATCCAAAGATGCCCCGAGAAGGTATGTTCCACCATGGGGTTCCCATCCCTGTGCCCCCACTGGAGGTGCTGAAACTTGGGGAGCAGAT
GACCCAGGAAGCCCGAGAGCATCTCTACCTCGTCCTCTTCTTTGACAACAAACGAACCTGGCAGTGGCTGCCCAGGACCAAGCTGGTTCCTCTGGGTGTG
AACCAGGACCTAGACAAGGAGAAGATGCTGGAGGGCCGCAAGTCCAACATCCGCAAGTCAGTACAGATCGCCTACCACAGGGCTCTGCAGCACCGCAGCA
AGGTGCAAGGCGAGCAGAGCAGTGAGACCAGCGATAGTGATTGATACTGCTCAACACAGCCCAACCTATAGTGCCCTGTGACTTCTCTCCTCCCCTTTGC
TCACTGTCCTGGAGTGGCACCGGCCTCTGCACTGACTCATTTCTGGTCTTGGGGCCAGTCTCAGGGGAAGCTGGGTGGGGGAGGTCCCTCCTGCCCTAAG
TGCAGCTGGACTGTACAGAACACTCCAAGGGCCAATGGCAGTTCAGCGCAAGGAGAGGGAGGGCCCACAGGTCAGAAAAAGCTCCAGAGACCTCACAGCA
TTGTAGGGCGGGGTGGTGGGCCAAAGTTAGGACACTGCGTAAAACAGGCCATCCCACCACCTCTACCTGCTCATGCCAGGAGAATCCATAACTGCCTAGA
GGCCTGGGGCCCCTACCGGTCGTGAGGTGAGTGGGCATCTGTCCAGCCTGGAAGAGGGGCACTAGGTGACTCCCTCCCCTGCTGTTGTAAATACTGTAAT
TATCGGAGAATTTAAATTATTCTCATTTGTAACTGCGTTTCCGGGTCGCGCCAGAGTCATTTGGTACTAAAAAAAAAAAAAAAAAAGACTGGGGGCTGTC
CCCATTTCCCTTCTCTTTCCCATAGATTCCCCCACCTTTCAAACTGGTTTGTATTTATTTCAAAGGAAGAAAATATATTGATTCTTAGAAAATAAACTGT
CAATTTAGAA
  • refseq_BRPF1.F2 (forward primer)
  • refseq_BRPF1.R2 (reverse primer)
  • Alternative donor

Frame 0

RRRRCCRRRCHSLCRHGLRRRCPRAASGSLHCS
SSARSRDRASWGLAKRAAADGVETPGRRRDQERQ
GSAQESRGAGTKAWGGRGPGWRGLRSPELGLPL
PGPTFPADLPKWGRSPGGRPRR*ARTRGGRCDS
MGVDFDVKTFCHNLRATKPPYECPVETCRKVYKS
YSGIEYHLYHYDHDNPPPPQQTPLRKHKKKGRQ
SRPANKQSPSPSEVSQSPGREVMSYAQAQRMVE
VDLHGRVHRISIFDNLDVVSEDEEAPEEAPENGS
NKENTETPAATPKSGKHKNKEKRKDSNHHHHHN
VSASTTPKLPEVVYRELEQDTPDAPPRPTSYYR
YIEKSAEELDEEVEYDMDEEDYIWLDIMNERRKT
EGVSPIPQEIFEYLMDRLEKESYFESHNKGDPN
ALVDEDAVCCICNDGECQNSNVILFCDMCNLAV
HQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCP
NKGGAFKQTDDGRWAHVVCALWIPEVCFANTVF
LEPIDSIEHIPPARWKLTCYICKQRGSGACIQC
HKANCYTAFHVTCAQQAGLYMKMEPVRETGANGT
SFSVRKTAYCDIHTPPGSARRLPALSHSEGEED
EDEEEDEGKGWSSEKVKKAKAKSRIKMKKARKI
LAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKS
QFMQRLHSYWTLKRQSRNGVPLLRRLQTHLQSQ
RNCDQVGRDSEDKNWALKEQLKSWQRLRHDLER
ARLLVELIRKREKLKRETIKVQQIAMEMQLTPFL
ILLRKTLEQLQEKDTGNIFSEPVPLSEVPDYLD
HIKKPMDFFTMKQNLEAYRYLNFDDFEEDFNLI
VSNCLKYNAKDTIFYRAAVRLREQGGAVLRQARR
QAEKMGIDFETGMHIPHSLAGDEATHHTEDAAE
EERLVLLENQKHLPVEEQLKLLLERLDEVNASK
QSVGRSRRAKMIKKEMTALRRKLAHQRETGRDGP
ERHGPSSRGSLTPHPAACDKDGQTDSAAEESSS
QETSKGLGPNMSSTPAHEVGRRTSVLFSKKNPK
TAGPPKRPGRPPKNRESQMTPSHGGSPVGPPQLP
IMSSLRQRKRGRSPRPSSSSDSDSDKSTEDPPM
DLPANGFSGGNQPVKKSFLVYRNDCSLPRSSSD
SESSSSSSSSAASDRTSTTPSKQGRGKPSFSRGT
FPEDSSEDTSGTENEAYSVGTGRGVGHSMVRKS
LGRGAGWLSEDEDSPLDALDLVWAKCRGYPSYP
ALIIDPKMPREGMFHHGVPIPVPPLEVLKLGEQM
TQEAREHLYLVLFFDNKRTWQWLPRTKLVPLGV
NQDLDKEKMLEGRKSNIRKSVQIAYHRALQHRS
KVQGEQSSETSDSD*YCSTQPNL*CPVTSLLPFA
HCPGVAPASALTHFWSWGQSQGKLGGGGPSCPK
CSWTVQNTPRANGSSAQGEGGPTGQKKLQRPHS
IVGRGGGPKLGHCVKQAIPPPLPAHARRIHNCLE
AWGPYRS*GEWASVQPGRGALGDSLPCCCKYCN
YRRI*IILICNCVSGSRQSHLVLKKKKKKTGGC
PHFPSLSHRFPHLSNWFVFISKEENILILRK*TV
NLE

Frame 1

VAAAAAAAAATAFAATVSAAAVLALPLGASIVP
AAPGPETGHLGGSRNGRRPTELRPQGAGGTRSG
RGQLRNPGEPGPKRGAAGGRGGEVYGLRSWGSPF
PARLSPPTSPSGVGAPAGAPGDEPGLEVADVTA
WGWTLM*RLSATTCGRLSHHTSARWRPAERSTR
VTVVLSTTCTTMTTTTHHPHNKLHSASTRKRGAS
HAQPTSSHPAPQRSHSHQAVR**AMHRPSAWWR
WTCMAASTASASLTTWMWCQRMRKPPRRPLRMA
ATRRTLRHQLLLPSQANIRTRRSARTPTITTTTM
FLRAPLPSCQRWSIGSWNRTPLMPHPGQLPITG
TSRSLQRSWTRK*SMTWTRRTTSGWIS*MSVGR
QRV*VPSRRRSLST*WTDWRRSRTLRVIIKATLM
R*WTRMLFAVSAMMVSARTAMSSSSVTCATWPC
TRSATVSPISLRASGCAAVACSHPLVLWIVPCA
PTRAVPSSRQMTGAGPMWCVPCGSLRSASPTRSS
*SLLTALSTSHQLAGSSPATFANNGAQGPASSA
TRPTVTQLSM*HAPSRLAFT*RWSLCGRQAPTA
PLSVSARQPTATSTRLQVQHADCLPCPTARVRRM
KMRRRMRVRAGAQRKSRRPRPSPGSK*RRHGRS
WQRSGQQHLWCQCPASHHTGLVKSPTA*PSKGR
ASSCRGCTATGH*SGSHGMGSHCYVACRHTCNLR
GTVTKLGEILRIRTGPLKNSSSPGSGSGMTWSE
LGCSWN*SASGKNSKGRRSRFSRLPWRCS*LLS
SSSFAKPWSSSKRRTQATSSASRSLCLRYLTT*T
TSKSPWTFSP*SRTWRLTAT*ILMILRRTSTSS
SATASSITPRTPSSTGQQCGFVSRVVLCSARPG
ARQKKWALTLRRACISPTAWLEMRPHTTLKMQPR
KSGWSCWRTRSTCQWKNS*SCFWSGWTK*MPAS
RVWAAHGVQR*SRKR*RHCGGSLPISERRDVMA
LSGMAPRAGVV*HPTRQPVTRMGRQIVRQRRAAA
RRQAKAWVPTCPQPPHMRWAGEPQFCSPKRTRR
QLDRPRGRAGPPKTGRAR*PPATEAVLWGPPSS
PS*VPCVSASGVGAPGPVRAQTATVISPQKTPQW
TYQPMASAVETNQ*RRVSWYTVMTAAFPGAAQT
LSPAAVAVAALLQTGPAQRPQNKAGANPPSLGA
LSQRTAVRIPQALRMRPTPWALAAAWATAW*GRV
WAGELAGCQRMRTPRWMLWTSCGPNAEAIHHTQ
L*SLIQRCPEKVCSTMGFPSLCPHWRC*NLGSR
*PRKPESISTSSSSLTTNEPGSGCPGPSWFLWV*
TRT*TRRRCWRAASPTSASQYRSPTTGLCSTAA
RCKASRAVRPAIVIDTAQHSPTYSAL*LLSSPL
LTVLEWHRPLH*LISGLGASLRGSWVGEVPPALS
AAGLYRTLQGPMAVQRKEREGPQVRKSSRDLTA
L*GGVVGQS*DTA*NRPSHHLYLLMPGESITA*
RPGAPTGREVSGHLSSLEEGH*VTPSPAVVNTVI
IGEFKLFSFVTAFPGRARVIWY*KKKKKRLGAV
PISLLFPIDSPTFQTGLYLFQRKKIY*FLENKL
SI*

Frame 2

SPPLLPPPLPQPLPPRSPPPLSSRCLWEPPLFQ
QRPVPRPGILGARETGGGRRS*DPRAPAGPGAA
GVSSGIPGSRDQSVGRQGAGVARSTVSGVGAPP
SRPDFPRRPPQVGSEPRRAPPAMSPDSRWPM*QH
GGGL*CEDFLPQLAGD*ATIRVPGGDLPKGLQE
LQWY*VPPVPL*PRQPTTPTTNSTPQAQEKGAP
VTPSQQAVTQPLRGLTVTRP*GDELCTGPAHGGG
GLAWPRPPHQHL*QPGCGVRG*GSPRGGP*EWQ
QQGEH*DTSCYSQVRQT*EQGEAQGLQPSPPPQ
CFCEHHSQAARGGLSGAGTGHP*CPTPANFLLPV
HREVCRGAGRGSRV*HGRGGLHLAGYHE*ASED
RGCKSHPAGDL*VPNGPTGEGVVL*ES**RRP*
CASGRGCCLLYLQ*W*VPEQQCHPLL*HVQPGRA
PGVLRCPLYP*GPVAVPPLPAVTLSCCGLCPVP
QQGRCLQADR*RALGPCGVCLVDP*GLLRQHGL
PRAY*QH*AHPTSSLEAHLLHLQTTGLRGLHPVP
QGQLLHSFPCDMRPAGWPLHEDGACAGDRRQRH
LFQCPQDSLLRHPHASRFSTPTACPVPQRG*GG
*R*GGG*G*GLELRESQEGQGQVPDQNEEGTEDP
GREAGSSTCGVSALHPTTQA**NHQPPDHPKEE
PVHAEAAQLLDTEAAVTEWGPIATSPADTPAIS
EEL*PSWERF*G*ELGP*RTAQVLAAAPA*LGAS
SAARGIDPQAGKTQKGDDQGSADCHGDAADSFP
HPPSQNLGAAPREGHRQHLQRAGPSV*GT*LPR
PHQKAHGLFHHEAELGGLPLPEF**F*GGLQPHR
QQLPQV*RQGHHLLPGSSAAS*AGWCCAPPGPA
PGRKNGH*L*DGHAYPPQPGWR*GHTPH*RCSR
GRAAGLAGEPEAPASGRTAKAASGAAGRSECQQA
ECGPLTACKDDQERDDGTAAEACPSARDGT*WP
*AAWPLEPG*SDTPPGSL*QGWADR*CGRGEQQ
PGDKQRPGSQHVLNPRT*GGQENLSSVLQKEPED
SWTAQEAGPAPQKPGEPDDPQPRRQSCGAPPAP
HHEFPASAQAG*EPPAQFELRQRQ**VHRRPPN
GLTSQWLQRWKPTSEEEFLGIP**LQPSPEQLRL
*VQQQ*Q*QRCFRPDQHNALKTRPGQTLLLSGH
FPRGQQ*GYLRH*E*GLLRGHWPRRGPQHGKEE
SGPGSWLAVRG*GLPAGCSGPRVGQMPRLSIIPS
SDH*SKDAPRRYVPPWGSHPCAPTGGAETWGAD
DPGSPRASLPRPLL*QQTNLAVAAQDQAGSSGC
EPGPRQGEDAGGPQVQHPQVSTDRLPQGSAAPQQ
GARRAEQ*DQR**LILLNTAQPIVPCDFSPPLC
SLSWSGTGLCTDSFLVLGPVSGEAGWGRSLLP*
VQLDCTEHSKGQWQFSARRGRAHRSEKAPETSQH
CRAGWWAKVRTLRKTGHPTTSTCSCQENP*LPR
GLGPLPVVR*VGICPAWKRGTR*LPPLLL*IL*
LSENLNYSHL*LRFRVAPESFGTKKKKKKDWGLS
PFPFSFP*IPPPFKLVCIYFKGRKYIDS*KINC
QFR

Sequence NM_001003694

CGTCGCCGCCGCTGCTGCCGCCGCCGCTGCCACAGCCTTTGCCGCCACGGTCTCCGCCGCCGCTGTCCTCGCGCTGCCTCTGGGAGCCTCCATTGTTCCA
GCAGCGCCCGGTCCCGAGACCGGGCATCTTGGGGGCTCGCGAAACGGGCGGCGGCCGACGGAGTTGAGACCCCAGGGCGCCGGCGGGACCAGGAGCGGCA
GGGGTCAGCTCAGGAATCCCGGGGAGCCGGGACCAAAGCGTGGGGCGGCAGGGGGCCGGGGTGGCGAGGTCTACGGTCTCCGGAGTTGGGGCTCCCCCTT
CCCGGCCCGACTTTCCCCGCCGACCTCCCCAAGTGGGGTCGGAGCCCCGGCGGGCGCCCCCGGCGATGAGCCCGGACTCGAGGTGGCCGATGTGACAGCA
TGGGGGTGGACTTTGATGTGAAGACTTTCTGCCACAACTTGCGGGCGACTAAGCCACCATACGAGTGCCCGGTGGAGACCTGCCGAAAGGTCTACAAGAG
TTACAGTGGTATTGAGTACCACCTGTACCACTATGACCACGACAACCCACCACCCCCACAACAAACTCCACTCCGCAAGCACAAGAAAAAGGGGCGCCAG
TCACGCCCAGCCAACAAGCAGTCACCCAGCCCCTCAGAGGTCTCACAGTCACCAGGCCGTGAGGTGATGAGCTATGCACAGGCCCAGCGCATGGTGGAGG
TGGACTTGCATGGCCGCGTCCACCGCATCAGCATCTTTGACAACCTGGATGTGGTGTCAGAGGATGAGGAAGCCCCCGAGGAGGCCCCTGAGAATGGCAG
CAACAAGGAGAACACTGAGACACCAGCTGCTACTCCCAAGTCAGGCAAACATAAGAACAAGGAGAAGCGCAAGGACTCCAACCATCACCACCACCACAAT
GTTTCTGCGAGCACCACTCCCAAGCTGCCAGAGGTGGTCTATCGGGAGCTGGAACAGGACACCCCTGATGCCCCACCCCGGCCAACTTCCTATTACCGGT
ACATCGAGAAGTCTGCAGAGGAGCTGGACGAGGAAGTAGAGTATGACATGGACGAGGAGGACTACATCTGGCTGGATATCATGAATGAGCGTCGGAAGAC
AGAGGGTGTAAGTCCCATCCCGCAGGAGATCTTTGAGTACCTAATGGACCGACTGGAGAAGGAGTCGTACTTTGAGAGTCATAATAAAGGCGACCCTAAT
GCGCTAGTGGACGAGGATGCTGTTTGCTGTATCTGCAATGATGGTGAGTGCCAGAACAGCAATGTCATCCTCTTCTGTGACATGTGCAACCTGGCCGTGC
ACCAGGAGTGCTACGGTGTCCCCTATATCCCTGAGGGCCAGTGGCTGTGCCGCCGTTGCCTGCAGTCACCCTCTCGTGCTGTGGATTGTGCCCTGTGCCC
CAACAAGGGCGGTGCCTTCAAGCAGACAGATGACGGGCGCTGGGCCCATGTGGTGTGTGCCTTGTGGATCCCTGAGGTCTGCTTCGCCAACACGGTCTTC
CTAGAGCCTATTGACAGCATTGAGCACATCCCACCAGCTCGCTGGAAGCTCACCTGCTACATTTGCAAACAACGGGGCTCAGGGGCCTGCATCCAGTGCC
ACAAGGCCAACTGTTACACAGCTTTCCATGTGACATGCGCCCAGCAGGCTGGCCTTTACATGAAGATGGAGCCTGTGCGGGAGACAGGCGCCAACGGCAC
CTCTTTCAGTGTCCGCAAGACAGCCTACTGCGACATCCACACGCCTCCAGGTTCAGCACGCCGACTGCCTGCCCTGTCCCACAGCGAGGGTGAGGAGGAT
GAAGATGAGGAGGAGGATGAGGGTAAGGGCTGGAGCTCAGAGAAAGTCAAGAAGGCCAAGGCCAAGTCCCGGATCAAAATGAAGAAGGCACGGAAGATCC
TGGCAGAGAAGCGGGCAGCAGCACCTGTGGTGTCAGTGCCCTGCATCCCACCACACAGGCTTAGTAAAATCACCAACCGCCTGACCATCCAAAGGAAGAG
CCAGTTCATGCAGAGGCTGCACAGCTACTGGACACTGAAGCGGCAGTCACGGAATGGGGTCCCATTGCTACGTCGCCTGCAGACACACCTGCAATCTCAG
AGGAACTGTGACCAAGTTGGGAGAGATTCTGAGGATAAGAACTGGGCCCTTAAAGAACAGCTCAAGTCCTGGCAGCGGCTCCGGCATGACTTGGAGCGAG
CTCGGCTGCTCGTGGAATTGATCCGCAAGCGGGAAAAACTCAAAAGGGAGACGATCAAGGTTCAGCAGATTGCCATGGAGATGCAGCTGACTCCTTTCCT
CATCCTCCTTCGCAAAACCTTGGAGCAGCTCCAAGAGAAGGACACAGGCAACATCTTCAGCGAGCCGGTCCCTCTGTCTGAGGTAACCGAATTGGACGAA
GTACCTGACTACCTAGACCACATCAAAAAGCCCATGGACTTTTTCACCATGAAGCAGAACTTGGAGGCTTACCGCTACCTGAATTTTGATGATTTTGAGG
AGGACTTCAACCTCATCGTCAGCAACTGCCTCAAGTATAACGCCAAGGACACCATCTTCTACCGGGCAGCAGTGCGGCTTCGTGAGCAGGGTGGTGCTGT
GCTCCGCCAGGCCCGGCGCCAGGCAGAAAAAATGGGCATTGACTTTGAGACGGGCATGCATATCCCCCACAGCCTGGCTGGAGATGAGGCCACACACCAC
ACTGAAGATGCAGCCGAGGAAGAGCGGCTGGTCTTGCTGGAGAACCAGAAGCACCTGCCAGTGGAAGAACAGCTAAAGCTGCTTCTGGAGCGGCTGGACG
AAGTGAATGCCAGCAAGCAGAGTGTGGGCCGCTCACGGCGTGCAAAGATGATCAAGAAAGAGATGACGGCACTGCGGCGGAAGCTTGCCCATCAGCGAGA
GACGGGACGTGATGGCCCTGAGCGGCATGGCCCCTCGAGCCGGGGTAGTCTGACACCCCACCCGGCAGCCTGTGACAAGGATGGGCAGACAGATAGTGCG
GCAGAGGAGAGCAGCAGCCAGGAGACAAGCAAAGGCCTGGGTCCCAACATGTCCTCAACCCCCGCACATGAGGTGGGCAGGAGAACCTCAGTTCTGTTCT
CCAAAAAGAACCCGAAGACAGCTGGACCGCCCAAGAGGCCGGGCCGGCCCCCCAAAAACCGGGAGAGCCAGATGACCCCCAGCCACGGAGGCAGTCCTGT
GGGGCCCCCCCAGCTCCCCATCATGAGTTCCCTGCGTCAGCGCAAGCGGGGTAGGAGCCCCCGGCCCAGTTCGAGCTCAGACAGCGACAGTGATAAGTCC
ACAGAAGACCCCCCAATGGACTTACCAGCCAATGGCTTCAGCGGTGGAAACCAACCAGTGAAGAAGAGTTTCTTGGTATACCGTAATGACTGCAGCCTTC
CCCGGAGCAGCTCAGACTCTGAGTCCAGCAGCAGTAGCAGTAGCAGCGCTGCTTCAGACCGGACCAGCACAACGCCCTCAAAACAAGGCCGGGGCAAACC
CTCCTTCTCTCGGGGCACTTTCCCAGAGGACAGCAGTGAGGATACCTCAGGCACTGAGAATGAGGCCTACTCCGTGGGCACTGGCCGCGGCGTGGGCCAC
AGCATGGTAAGGAAGAGTCTGGGCCGGGGAGCTGGCTGGCTGTCAGAGGATGAGGACTCCCCGCTGGATGCTCTGGACCTCGTGTGGGCCAAATGCCGAG
GCTATCCATCATACCCAGCTCTGATCATTGATCCAAAGATGCCCCGAGAAGGTATGTTCCACCATGGGGTTCCCATCCCTGTGCCCCCACTGGAGGTGCT
GAAACTTGGGGAGCAGATGACCCAGGAAGCCCGAGAGCATCTCTACCTCGTCCTCTTCTTTGACAACAAACGAACCTGGCAGTGGCTGCCCAGGACCAAG
CTGGTTCCTCTGGGTGTGAACCAGGACCTAGACAAGGAGAAGATGCTGGAGGGCCGCAAGTCCAACATCCGCAAGTCAGTACAGATCGCCTACCACAGGG
CTCTGCAGCACCGCAGCAAGGTGCAAGGCGAGCAGAGCAGTGAGACCAGCGATAGTGATTGATACTGCTCAACACAGCCCAACCTATAGTGCCCTGTGAC
TTCTCTCCTCCCCTTTGCTCACTGTCCTGGAGTGGCACCGGCCTCTGCACTGACTCATTTCTGGTCTTGGGGCCAGTCTCAGGGGAAGCTGGGTGGGGGA
GGTCCCTCCTGCCCTAAGTGCAGCTGGACTGTACAGAACACTCCAAGGGCCAATGGCAGTTCAGCGCAAGGAGAGGGAGGGCCCACAGGTCAGAAAAAGC
TCCAGAGACCTCACAGCATTGTAGGGCGGGGTGGTGGGCCAAAGTTAGGACACTGCGTAAAACAGGCCATCCCACCACCTCTACCTGCTCATGCCAGGAG
AATCCATAACTGCCTAGAGGCCTGGGGCCCCTACCGGTCGTGAGGTGAGTGGGCATCTGTCCAGCCTGGAAGAGGGGCACTAGGTGACTCCCTCCCCTGC
TGTTGTAAATACTGTAATTATCGGAGAATTTAAATTATTCTCATTTGTAACTGCGTTTCCGGGTCGCGCCAGAGTCATTTGGTACTAAAAAAAAAAAAAA
AAAAGACTGGGGGCTGTCCCCATTTCCCTTCTCTTTCCCATAGATTCCCCCACCTTTCAAACTGGTTTGTATTTATTTCAAAGGAAGAAAATATATTGAT
TCTTAGAAAATAAACTGTCAATTTAGAA
  • refseq_BRPF1.F2 (forward primer)
  • refseq_BRPF1.R2 (reverse primer)
  • Alternative donor

Frame 0

RRRRCCRRRCHSLCRHGLRRRCPRAASGSLHCS
SSARSRDRASWGLAKRAAADGVETPGRRRDQERQ
GSAQESRGAGTKAWGGRGPGWRGLRSPELGLPL
PGPTFPADLPKWGRSPGGRPRR*ARTRGGRCDS
MGVDFDVKTFCHNLRATKPPYECPVETCRKVYKS
YSGIEYHLYHYDHDNPPPPQQTPLRKHKKKGRQ
SRPANKQSPSPSEVSQSPGREVMSYAQAQRMVE
VDLHGRVHRISIFDNLDVVSEDEEAPEEAPENGS
NKENTETPAATPKSGKHKNKEKRKDSNHHHHHN
VSASTTPKLPEVVYRELEQDTPDAPPRPTSYYR
YIEKSAEELDEEVEYDMDEEDYIWLDIMNERRKT
EGVSPIPQEIFEYLMDRLEKESYFESHNKGDPN
ALVDEDAVCCICNDGECQNSNVILFCDMCNLAV
HQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCP
NKGGAFKQTDDGRWAHVVCALWIPEVCFANTVF
LEPIDSIEHIPPARWKLTCYICKQRGSGACIQC
HKANCYTAFHVTCAQQAGLYMKMEPVRETGANGT
SFSVRKTAYCDIHTPPGSARRLPALSHSEGEED
EDEEEDEGKGWSSEKVKKAKAKSRIKMKKARKI
LAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKS
QFMQRLHSYWTLKRQSRNGVPLLRRLQTHLQSQ
RNCDQVGRDSEDKNWALKEQLKSWQRLRHDLER
ARLLVELIRKREKLKRETIKVQQIAMEMQLTPFL
ILLRKTLEQLQEKDTGNIFSEPVPLSEVTELDE
VPDYLDHIKKPMDFFTMKQNLEAYRYLNFDDFE
EDFNLIVSNCLKYNAKDTIFYRAAVRLREQGGAV
LRQARRQAEKMGIDFETGMHIPHSLAGDEATHH
TEDAAEEERLVLLENQKHLPVEEQLKLLLERLD
EVNASKQSVGRSRRAKMIKKEMTALRRKLAHQRE
TGRDGPERHGPSSRGSLTPHPAACDKDGQTDSA
AEESSSQETSKGLGPNMSSTPAHEVGRRTSVLF
SKKNPKTAGPPKRPGRPPKNRESQMTPSHGGSPV
GPPQLPIMSSLRQRKRGRSPRPSSSSDSDSDKS
TEDPPMDLPANGFSGGNQPVKKSFLVYRNDCSL
PRSSSDSESSSSSSSSAASDRTSTTPSKQGRGKP
SFSRGTFPEDSSEDTSGTENEAYSVGTGRGVGH
SMVRKSLGRGAGWLSEDEDSPLDALDLVWAKCR
GYPSYPALIIDPKMPREGMFHHGVPIPVPPLEVL
KLGEQMTQEAREHLYLVLFFDNKRTWQWLPRTK
LVPLGVNQDLDKEKMLEGRKSNIRKSVQIAYHR
ALQHRSKVQGEQSSETSDSD*YCSTQPNL*CPVT
SLLPFAHCPGVAPASALTHFWSWGQSQGKLGGG
GPSCPKCSWTVQNTPRANGSSAQGEGGPTGQKK
LQRPHSIVGRGGGPKLGHCVKQAIPPPLPAHARR
IHNCLEAWGPYRS*GEWASVQPGRGALGDSLPC
CCKYCNYRRI*IILICNCVSGSRQSHLVLKKKK
KKTGGCPHFPSLSHRFPHLSNWFVFISKEENILI
LRK*TVNLE

Frame 1

VAAAAAAAAATAFAATVSAAAVLALPLGASIVP
AAPGPETGHLGGSRNGRRPTELRPQGAGGTRSG
RGQLRNPGEPGPKRGAAGGRGGEVYGLRSWGSPF
PARLSPPTSPSGVGAPAGAPGDEPGLEVADVTA
WGWTLM*RLSATTCGRLSHHTSARWRPAERSTR
VTVVLSTTCTTMTTTTHHPHNKLHSASTRKRGAS
HAQPTSSHPAPQRSHSHQAVR**AMHRPSAWWR
WTCMAASTASASLTTWMWCQRMRKPPRRPLRMA
ATRRTLRHQLLLPSQANIRTRRSARTPTITTTTM
FLRAPLPSCQRWSIGSWNRTPLMPHPGQLPITG
TSRSLQRSWTRK*SMTWTRRTTSGWIS*MSVGR
QRV*VPSRRRSLST*WTDWRRSRTLRVIIKATLM
R*WTRMLFAVSAMMVSARTAMSSSSVTCATWPC
TRSATVSPISLRASGCAAVACSHPLVLWIVPCA
PTRAVPSSRQMTGAGPMWCVPCGSLRSASPTRSS
*SLLTALSTSHQLAGSSPATFANNGAQGPASSA
TRPTVTQLSM*HAPSRLAFT*RWSLCGRQAPTA
PLSVSARQPTATSTRLQVQHADCLPCPTARVRRM
KMRRRMRVRAGAQRKSRRPRPSPGSK*RRHGRS
WQRSGQQHLWCQCPASHHTGLVKSPTA*PSKGR
ASSCRGCTATGH*SGSHGMGSHCYVACRHTCNLR
GTVTKLGEILRIRTGPLKNSSSPGSGSGMTWSE
LGCSWN*SASGKNSKGRRSRFSRLPWRCS*LLS
SSSFAKPWSSSKRRTQATSSASRSLCLR*PNWTK
YLTT*TTSKSPWTFSP*SRTWRLTAT*ILMILR
RTSTSSSATASSITPRTPSSTGQQCGFVSRVVL
CSARPGARQKKWALTLRRACISPTAWLEMRPHTT
LKMQPRKSGWSCWRTRSTCQWKNS*SCFWSGWT
K*MPASRVWAAHGVQR*SRKR*RHCGGSLPISE
RRDVMALSGMAPRAGVV*HPTRQPVTRMGRQIVR
QRRAAARRQAKAWVPTCPQPPHMRWAGEPQFCS
PKRTRRQLDRPRGRAGPPKTGRAR*PPATEAVL
WGPPSSPS*VPCVSASGVGAPGPVRAQTATVISP
QKTPQWTYQPMASAVETNQ*RRVSWYTVMTAAF
PGAAQTLSPAAVAVAALLQTGPAQRPQNKAGAN
PPSLGALSQRTAVRIPQALRMRPTPWALAAAWAT
AW*GRVWAGELAGCQRMRTPRWMLWTSCGPNAE
AIHHTQL*SLIQRCPEKVCSTMGFPSLCPHWRC
*NLGSR*PRKPESISTSSSSLTTNEPGSGCPGPS
WFLWV*TRT*TRRRCWRAASPTSASQYRSPTTG
LCSTAARCKASRAVRPAIVIDTAQHSPTYSAL*
LLSSPLLTVLEWHRPLH*LISGLGASLRGSWVGE
VPPALSAAGLYRTLQGPMAVQRKEREGPQVRKS
SRDLTAL*GGVVGQS*DTA*NRPSHHLYLLMPG
ESITA*RPGAPTGREVSGHLSSLEEGH*VTPSPA
VVNTVIIGEFKLFSFVTAFPGRARVIWY*KKKK
KRLGAVPISLLFPIDSPTFQTGLYLFQRKKIY*
FLENKLSI*

Frame 2

SPPLLPPPLPQPLPPRSPPPLSSRCLWEPPLFQ
QRPVPRPGILGARETGGGRRS*DPRAPAGPGAA
GVSSGIPGSRDQSVGRQGAGVARSTVSGVGAPP
SRPDFPRRPPQVGSEPRRAPPAMSPDSRWPM*QH
GGGL*CEDFLPQLAGD*ATIRVPGGDLPKGLQE
LQWY*VPPVPL*PRQPTTPTTNSTPQAQEKGAP
VTPSQQAVTQPLRGLTVTRP*GDELCTGPAHGGG
GLAWPRPPHQHL*QPGCGVRG*GSPRGGP*EWQ
QQGEH*DTSCYSQVRQT*EQGEAQGLQPSPPPQ
CFCEHHSQAARGGLSGAGTGHP*CPTPANFLLPV
HREVCRGAGRGSRV*HGRGGLHLAGYHE*ASED
RGCKSHPAGDL*VPNGPTGEGVVL*ES**RRP*
CASGRGCCLLYLQ*W*VPEQQCHPLL*HVQPGRA
PGVLRCPLYP*GPVAVPPLPAVTLSCCGLCPVP
QQGRCLQADR*RALGPCGVCLVDP*GLLRQHGL
PRAY*QH*AHPTSSLEAHLLHLQTTGLRGLHPVP
QGQLLHSFPCDMRPAGWPLHEDGACAGDRRQRH
LFQCPQDSLLRHPHASRFSTPTACPVPQRG*GG
*R*GGG*G*GLELRESQEGQGQVPDQNEEGTEDP
GREAGSSTCGVSALHPTTQA**NHQPPDHPKEE
PVHAEAAQLLDTEAAVTEWGPIATSPADTPAIS
EEL*PSWERF*G*ELGP*RTAQVLAAAPA*LGAS
SAARGIDPQAGKTQKGDDQGSADCHGDAADSFP
HPPSQNLGAAPREGHRQHLQRAGPSV*GNRIGR
ST*LPRPHQKAHGLFHHEAELGGLPLPEF**F*G
GLQPHRQQLPQV*RQGHHLLPGSSAAS*AGWCC
APPGPAPGRKNGH*L*DGHAYPPQPGWR*GHTP
H*RCSRGRAAGLAGEPEAPASGRTAKAASGAAGR
SECQQAECGPLTACKDDQERDDGTAAEACPSAR
DGT*WP*AAWPLEPG*SDTPPGSL*QGWADR*C
GRGEQQPGDKQRPGSQHVLNPRT*GGQENLSSVL
QKEPEDSWTAQEAGPAPQKPGEPDDPQPRRQSC
GAPPAPHHEFPASAQAG*EPPAQFELRQRQ**V
HRRPPNGLTSQWLQRWKPTSEEEFLGIP**LQPS
PEQLRL*VQQQ*Q*QRCFRPDQHNALKTRPGQT
LLLSGHFPRGQQ*GYLRH*E*GLLRGHWPRRGP
QHGKEESGPGSWLAVRG*GLPAGCSGPRVGQMPR
LSIIPSSDH*SKDAPRRYVPPWGSHPCAPTGGA
ETWGADDPGSPRASLPRPLL*QQTNLAVAAQDQ
AGSSGCEPGPRQGEDAGGPQVQHPQVSTDRLPQG
SAAPQQGARRAEQ*DQR**LILLNTAQPIVPCD
FSPPLCSLSWSGTGLCTDSFLVLGPVSGEAGWG
RSLLP*VQLDCTEHSKGQWQFSARRGRAHRSEKA
PETSQHCRAGWWAKVRTLRKTGHPTTSTCSCQE
NP*LPRGLGPLPVVR*VGICPAWKRGTR*LPPL
LL*IL*LSENLNYSHL*LRFRVAPESFGTKKKKK
KDWGLSPFPFSFP*IPPPFKLVCIYFKGRKYID
S*KINCQFR

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