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Sequence NM_001724
GCCCACGTCTGGTTCAGGGGCTAGAAAAGAGCGTCGATGCCGGCGGCAGTGATGAGTCCTAGGAGGCGCTGGCTCTTTGGCGGCTCGGAGGAGCGGCTGCTGCTGCTGCTGCTGCTGCTGGTGGCCCCTTTGCAGATGTATTGCTGTCCTTGAATATTAGCCCATTTGAAAACGCCTGGGAAGTTCAGCCATCAGTATGT
CCAAGTACAAACTTATTATGTTAAGACATGGAGAGGGTGCTTGGAATAAGGAGAACCGTTTTTGTAGCTGGGTGGATCAGAAACTCAACAGCGAAGGAAT
GGAGGAAGCTCGGAACTGTGGGAAGCAACTCAAAGCGTTAAACTTTGAGTTTGATCTTGTATTCACATCTGTCCTTAATCGGTCCATTCACACAGCCTGG
CTGATCCTGGAAGAGCTAGGCCAGGAATGGGTGCCTGTGGAAAGCTCCTGGCGTCTAAATGAGCGTCACTATGGGGCCTTGATCGGTCTCAACAGGGAGC
AGATGGCTTTGAATCATGGTGAAGAACAAGTGAGGCTCTGGAGAAGAAGCTACAATGTAACCCCGCCTCCCATTGAGGAGTCTCATCCTTACTACCAAGA
AATCTACAACGACCGGAGGTATAAAGTATGCGATGTGCCCTTGGATCAACTGCCACGGTCGGAAAGCTTAAAGGATGTTCTGGAGAGACTCCTTCCCTAT
TGGAATGAAAGGATTGCTCCCGAAGTATTACGTGGCAAAACCATTCTGATATCTGCTCATGGAAATAGCAGTAGGGCACTCCTAAAACACCTGGAAGGTA
TCTCAGATGAAGACATCATCAACATTACTCTTCCTACTGGAGTCCCCATTCTTCTGGAATTGGATGAAAACCTGCGTGCTGTTGGGCCTCATCAGTTCCT
GGGTGACCAAGAGGCGATCCAAGCAGCCATTAAGAAAGTAGAAGATCAAGGAAAAGTGAAACAAGCTAAAAAATAGTCTTTCTCAACTGTTGGCTAAGAA
GAAATGCAAAAGAAGTGGCATAGGAGTGTGTTATGGGTGCTGAACTCTCTCTCTTTTTCCCCGATTTTCCAGAGCTAGGCTGTGGAGTAGAGTTTGTATA
GGTAACTAGGTAACTTATTGTGGCCCAGATAAGGCTTTAGGATGCCTCAGTGCTTATGTCATAGCCTTATGAGTTAGCTTTCTTGCTAGCCCCCTAGTCG
GTCACCAAACTAGTAACTAGTGGGGCTTAATGAAGGTCATAAGTTTCTGAGATGGGAGAGCAACAAGTAGAGATGAAGTTAAAGGTATTTATCATTCAAG
AAATCATTATTGAGTCACCATTGACAGGCACTATTCTAATCAGTAGTTCACTTTAATATTTAATAAGATTTTCTGGGATAACAGTAAGGGATATTAGATA
ATATACCGTATGTATTTATTACTAGTCTTTTCCTCTAGGAAAAGGGATACTTTGATAATTAAGGCCAGAGGCCCATTAGTTGAGAAAGTCACAGATATAT
TTCTCCAAGAAAGCCAACAACCACCACCACAATGACAGAAATGACAACAAGGCCCTTTAACTTGTCTTCTAGTTTAGAGACATCCTTCATTTGACATTTA
GTAGAATTCCTCTTTGGCCACAAGAATAAGCAGCAAATAAACAACTATGGCTGTTGAGGTTCTCATTTTGGTTTGTTTTAATTTTTTGAACTTTGGGTAC
CTGTAATTAGTTTAAAAATAAAGTTCCTGATAATAAAGTGACTGAAAATGGCATCCCCAAA
- refseq_BPGM.F1 (forward primer)
- refseq_BPGM.R1 (reverse primer)
- Single exon skipping
Frame 0
AHVWFRG*KRASMPAAVMSPRRRWLFGGSEERLLLLLLLLVAPLQMYCCP*ILAHLKTPGKFSHQYV
QVQTYYVKTWRGCLE*GEPFL*LGGSETQQRRN
GGSSELWEATQSVKL*V*SCIHICP*SVHSHSL
ADPGRARPGMGACGKLLASK*ASLWGLDRSQQGA
DGFESW*RTSEALEKKLQCNPASH*GVSSLLPR
NLQRPEV*SMRCALGSTATVGKLKGCSGETPSL
LE*KDCSRSITWQNHSDICSWK*Q*GTPKTPGRY
LR*RHHQHYSSYWSPHSSGIG*KPACCWASSVP
G*PRGDPSSH*ESRRSRKSETS*KIVFLNCWLR
RNAKEVA*ECVMGAELSLFFPDFPELGCGVEFV*
VTR*LIVAQIRL*DASVLMS*PYELAFLLAP*S
VTKLVTSGA**RS*VSEMGEQQVEMKLKVFIIQ
EIIIESPLTGTILISSSL*YLIRFSGITVRDIR*
YTVCIYY*SFPLGKGIL**LRPEAH*LRKSQIY
FSKKANNHHHNDRNDNKAL*LVF*FRDILHLTF
SRIPLWPQE*AANKQLWLLRFSFWFVLIF*TLGT
CN*FKNKVPDNKVTENGIPK
Frame 1
PTSGSGARKERRCRRQ**VLGGAGSLAARRSGCCCCCCCWWPLCRCIAVLEY*PI*KRLGSSAISM
SKYKLIMLRHGEGAWNKENRFCSWVDQKLNSEGM
EEARNCGKQLKALNFEFDLVFTSVLNRSIHTAW
LILEELGQEWVPVESSWRLNERHYGALIGLNRE
QMALNHGEEQVRLWRRSYNVTPPPIEESHPYYQE
IYNDRRYKVCDVPLDQLPRSESLKDVLERLLPY
WNERIAPEVLRGKTILISAHGNSSRALLKHLEG
ISDEDIINITLPTGVPILLELDENLRAVGPHQFL
GDQEAIQAAIKKVEDQGKVKQAKK*SFSTVG*E
EMQKKWHRSVLWVLNSLSFSPIFQS*AVE*SLY
R*LGNLLWPR*GFRMPQCLCHSLMS*LSC*PPSR
SPN**LVGLNEGHKFLRWESNK*R*S*RYLSFK
KSLLSHH*QALF*SVVHFNI**DFLG*Q*GILD
NIPYVFITSLFL*EKGYFDN*GQRPIS*ESHRYI
SPRKPTTTTTMTEMTTRPFNLSSSLETSFI*HL
VEFLFGHKNKQQINNYGC*GSHFGLF*FFELWV
PVISLKIKFLIIK*LKMASP
Frame 2
PRLVQGLEKSVDAGGSDES*EALALWRLGGAAAAAAAAAGGPFADVLLSLNISPFENAWEVQPSVC
PSTNLLC*DMERVLGIRRTVFVAGWIRNSTAKE
WRKLGTVGSNSKR*TLSLILYSHLSLIGPFTQPG
*SWKS*ARNGCLWKAPGV*MSVTMGP*SVSTGS
RWL*IMVKNK*GSGEEATM*PRLPLRSLILTTK
KSTTTGGIKYAMCPWINCHGRKA*RMFWRDSFPI
GMKGLLPKYYVAKPF*YLLMEIAVGHS*NTWKV
SQMKTSSTLLFLLESPFFWNWMKTCVLLGLISS
WVTKRRSKQPLRK*KIKEK*NKLKNSLSQLLAKK
KCKRSGIGVCYGC*TLSLFPRFSRARLWSRVCI
GN*VTYCGPDKALGCLSAYVIAL*VSFLASPLV
GHQTSN*WGLMKVISF*DGRATSRDEVKGIYHSR
NHY*VTIDRHYSNQ*FTLIFNKIFWDNSKGY*I
IYRMYLLLVFSSRKRDTLIIKARGPLVEKVTDI
FLQESQQPPPQ*QK*QQGPLTCLLV*RHPSFDI*
*NSSLATRISSK*TTMAVEVLILVCFNFLNFGY
L*LV*K*SS***SD*KWHPQ
Sequence NM_199186
GCCCACGTCTGGTTCAGGGGCTAGAAAAGAGCGTCGATGCCGGCGGCAGTGATGAGTCCTAGGAGGCGCTGGCTCTTTGGCGGCTCGGAGGAGCGGCTGCTGCTGCTGCTGCTGCTGCTGGTGGCCCCTTTGCAGAAAAGTTCTAAGAACAGTGCTGTTGGCTCAGTGAGATGAGGTTATCGGAAAACTTTCTGTTTACA
CAAGAGTTGCCTCCTTCAAAGAAGCATGCATGGTCCTTGCAGGTCACTGTGCCCATCACTCTGCATAGTCCAGCTGCACTCTGCTTCACACAGCTGCCTG
CCCATTGCAGGCATTTGAGTTTGCAATCCATGCCTTAAACTTTAAATCACACTGGCTACAGCGTTGTGTGTTTCTTGGTAAACACTGGGTAAATCAGCTC
CTGTGGATGTTGACTGAAGTTTCTATGTTTGCAAGCTAATCCTCAGCAGTTTCTTTATGTATTGCTGTCCTTGAATATTAGCCCATTTGAAAACGCCTGG
GAAGTTCAGCCATCAGTATGTCCAAGTACAAACTTATTATGTTAAGACATGGAGAGGGTGCTTGGAATAAGGAGAACCGTTTTTGTAGCTGGGTGGATCA
GAAACTCAACAGCGAAGGAATGGAGGAAGCTCGGAACTGTGGGAAGCAACTCAAAGCGTTAAACTTTGAGTTTGATCTTGTATTCACATCTGTCCTTAAT
CGGTCCATTCACACAGCCTGGCTGATCCTGGAAGAGCTAGGCCAGGAATGGGTGCCTGTGGAAAGCTCCTGGCGTCTAAATGAGCGTCACTATGGGGCCT
TGATCGGTCTCAACAGGGAGCAGATGGCTTTGAATCATGGTGAAGAACAAGTGAGGCTCTGGAGAAGAAGCTACAATGTAACCCCGCCTCCCATTGAGGA
GTCTCATCCTTACTACCAAGAAATCTACAACGACCGGAGGTATAAAGTATGCGATGTGCCCTTGGATCAACTGCCACGGTCGGAAAGCTTAAAGGATGTT
CTGGAGAGACTCCTTCCCTATTGGAATGAAAGGATTGCTCCCGAAGTATTACGTGGCAAAACCATTCTGATATCTGCTCATGGAAATAGCAGTAGGGCAC
TCCTAAAACACCTGGAAGGTATCTCAGATGAAGACATCATCAACATTACTCTTCCTACTGGAGTCCCCATTCTTCTGGAATTGGATGAAAACCTGCGTGC
TGTTGGGCCTCATCAGTTCCTGGGTGACCAAGAGGCGATCCAAGCAGCCATTAAGAAAGTAGAAGATCAAGGAAAAGTGAAACAAGCTAAAAAATAGTCT
TTCTCAACTGTTGGCTAAGAAGAAATGCAAAAGAAGTGGCATAGGAGTGTGTTATGGGTGCTGAACTCTCTCTCTTTTTCCCCGATTTTCCAGAGCTAGG
CTGTGGAGTAGAGTTTGTATAGGTAACTAGGTAACTTATTGTGGCCCAGATAAGGCTTTAGGATGCCTCAGTGCTTATGTCATAGCCTTATGAGTTAGCT
TTCTTGCTAGCCCCCTAGTCGGTCACCAAACTAGTAACTAGTGGGGCTTAATGAAGGTCATAAGTTTCTGAGATGGGAGAGCAACAAGTAGAGATGAAGT
TAAAGGTATTTATCATTCAAGAAATCATTATTGAGTCACCATTGACAGGCACTATTCTAATCAGTAGTTCACTTTAATATTTAATAAGATTTTCTGGGAT
AACAGTAAGGGATATTAGATAATATACCGTATGTATTTATTACTAGTCTTTTCCTCTAGGAAAAGGGATACTTTGATAATTAAGGCCAGAGGCCCATTAG
TTGAGAAAGTCACAGATATATTTCTCCAAGAAAGCCAACAACCACCACCACAATGACAGAAATGACAACAAGGCCCTTTAACTTGTCTTCTAGTTTAGAG
ACATCCTTCATTTGACATTTAGTAGAATTCCTCTTTGGCCACAAGAATAAGCAGCAAATAAACAACTATGGCTGTTGAGGTTCTCATTTTGGTTTGTTTT
AATTTTTTGAACTTTGGGTACCTGTAATTAGTTTAAAAATAAAGTTCCTGATAATAAAGTGACTGAAAATGGCATCCCCAAA
- refseq_BPGM.F1 (forward primer)
- refseq_BPGM.R1 (reverse primer)
- Single exon skipping
Frame 0
AHVWFRG*KRASMPAAVMSPRRRWLFGGSEERLLLLLLLLVAPLQKSSKNSAVGSVR*GYRKTFCLH
KSCLLQRSMHGPCRSLCPSLCIVQLHSASHSCL
PIAGI*VCNPCLKL*ITLATALCVSW*TLGKSA
PVDVD*SFYVCKLILSSFFMYCCP*ILAHLKTPG
KFSHQYVQVQTYYVKTWRGCLE*GEPFL*LGGS
ETQQRRNGGSSELWEATQSVKL*V*SCIHICP*
SVHSHSLADPGRARPGMGACGKLLASK*ASLWGL
DRSQQGADGFESW*RTSEALEKKLQCNPASH*G
VSSLLPRNLQRPEV*SMRCALGSTATVGKLKGC
SGETPSLLE*KDCSRSITWQNHSDICSWK*Q*GT
PKTPGRYLR*RHHQHYSSYWSPHSSGIG*KPAC
CWASSVPG*PRGDPSSH*ESRRSRKSETS*KIV
FLNCWLRRNAKEVA*ECVMGAELSLFFPDFPELG
CGVEFV*VTR*LIVAQIRL*DASVLMS*PYELA
FLLAP*SVTKLVTSGA**RS*VSEMGEQQVEMK
LKVFIIQEIIIESPLTGTILISSSL*YLIRFSGI
TVRDIR*YTVCIYY*SFPLGKGIL**LRPEAH*
LRKSQIYFSKKANNHHHNDRNDNKAL*LVF*FR
DILHLTFSRIPLWPQE*AANKQLWLLRFSFWFVL
IF*TLGTCN*FKNKVPDNKVTENGIPK
Frame 1
PTSGSGARKERRCRRQ**VLGGAGSLAARRSGCCCCCCCWWPLCRKVLRTVLLAQ*DEVIGKLSVY
TRVASFKEACMVLAGHCAHHSA*SSCTLLHTAAC
PLQAFEFAIHALNFKSHWLQRCVFLGKHWVNQL
LWMLTEVSMFAS*SSAVSLCIAVLEY*PI*KRL
GSSAISMSKYKLIMLRHGEGAWNKENRFCSWVDQ
KLNSEGMEEARNCGKQLKALNFEFDLVFTSVLN
RSIHTAWLILEELGQEWVPVESSWRLNERHYGA
LIGLNREQMALNHGEEQVRLWRRSYNVTPPPIEE
SHPYYQEIYNDRRYKVCDVPLDQLPRSESLKDV
LERLLPYWNERIAPEVLRGKTILISAHGNSSRA
LLKHLEGISDEDIINITLPTGVPILLELDENLRA
VGPHQFLGDQEAIQAAIKKVEDQGKVKQAKK*S
FSTVG*EEMQKKWHRSVLWVLNSLSFSPIFQS*
AVE*SLYR*LGNLLWPR*GFRMPQCLCHSLMS*L
SC*PPSRSPN**LVGLNEGHKFLRWESNK*R*S
*RYLSFKKSLLSHH*QALF*SVVHFNI**DFLG
*Q*GILDNIPYVFITSLFL*EKGYFDN*GQRPIS
*ESHRYISPRKPTTTTTMTEMTTRPFNLSSSLE
TSFI*HLVEFLFGHKNKQQINNYGC*GSHFGLF
*FFELWVPVISLKIKFLIIK*LKMASP
Frame 2
PRLVQGLEKSVDAGGSDES*EALALWRLGGAAAAAAAAAGGPFAEKF*EQCCWLSEMRLSENFLFT
QELPPSKKHAWSLQVTVPITLHSPAALCFTQLP
AHCRHLSLQSMP*TLNHTGYSVVCFLVNTG*ISS
CGC*LKFLCLQANPQQFLYVLLSLNISPFENAW
EVQPSVCPSTNLLC*DMERVLGIRRTVFVAGWI
RNSTAKEWRKLGTVGSNSKR*TLSLILYSHLSLI
GPFTQPG*SWKS*ARNGCLWKAPGV*MSVTMGP
*SVSTGSRWL*IMVKNK*GSGEEATM*PRLPLR
SLILTTKKSTTTGGIKYAMCPWINCHGRKA*RMF
WRDSFPIGMKGLLPKYYVAKPF*YLLMEIAVGH
S*NTWKVSQMKTSSTLLFLLESPFFWNWMKTCV
LLGLISSWVTKRRSKQPLRK*KIKEK*NKLKNSL
SQLLAKKKCKRSGIGVCYGC*TLSLFPRFSRAR
LWSRVCIGN*VTYCGPDKALGCLSAYVIAL*VS
FLASPLVGHQTSN*WGLMKVISF*DGRATSRDEV
KGIYHSRNHY*VTIDRHYSNQ*FTLIFNKIFWD
NSKGY*IIYRMYLLLVFSSRKRDTLIIKARGPL
VEKVTDIFLQESQQPPPQ*QK*QQGPLTCLLV*R
HPSFDI**NSSLATRISSK*TTMAVEVLILVCF
NFLNFGYL*LV*K*SS***SD*KWHPQ
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