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Sequences

Sequence NM_144782

ATCGATGCCCCGTCCCCGCCCCACAGCCAACCTCCCGGGCACCGTTGCCCGCCCGGGGCCCGCTACCGGCCCAGGCCCCGCCTCGCGAGTCCTCCTCCCC GGGTGCCTTCCCGCAGCCCGCTCGGCCCAGAGGGTGGGCGCGGGGCTGCCTCTACCGGCTGGCGGCTGTAACTCAGCGACCTTGGCCCGAAGGCTCTAGC AAGGACCCACCGACCCCAGCCGCGGCGGCGGCGGCGCGGACTTTGCCCGGTGTGTGGGGCGGAGCGGACTGCGTGTCCGCGGACGGGCAGCGAAGATGTT AGCCTTCGCTGCCAGGACCGTGGTGAAGCCTCTGGGCTTCCTGAAGCCCTTCTCCTTGATGAAGGCTTCCAGCCGCTTCAAGGCACACCAGGATGCACTG CCACGGCTGCCCGTGCCCCCTCTCCAGCAGTCCCTGGACCACTACCTGAAGGCGCTGCAGCCCATCGTGAGTGAGGAGGAGTGGGCCCACACCAAGCAGC TGGTGGATGAGTTTCAGGCCTCAGGAGGTGTAGGGGAGCGCCTGCAGAAGGGGCTGGAGCGTCGGGCCAGGAAGACGGAGAACTGGCTGTCTGAGTGGTG GCTCAAGACCGCCTACCTCCAGTACCGCCAGCCTGTGGTCATCTACTCGAGCCCAGGCGTGATGCTACCCAAGCAGGACTTCGTGGACCTGCAGGGTCAG CTCCGATTTGCTGCCAAACTCATTGAGGGTGTGTTGGATTTCAAGGTCATGATTGACAACGAGACCCTGCCCGTGGAGTACCTGGGGGGGAAGCCACTGT GCATGAACCAGTACTATCAGATCTTGTCCTCCTGCCGAGTGCCGGGCCCCAAGCAGGACACAGTCAGCAACTTCAGCAAGACCAAGAAGCCTCCCACGCA CATCACCGTGGTACACAACTACCAGTTTTTTGAGCTGGATGTGTACCACAGTGACGGGACACCCCTCACTGCGGATCAGATCTTTGTGCAGCTGGAGAAG ATCTGGAACTCATCCCTACAGACCAACAAGGAGCCTGTGGGCATCCTCACCTCCAACCACCGCAACTCCTGGGCCAAGGCATACAACACCCTCATCAAAG ACAAGGTGAACCGGGATTCCGTGCGCTCCATCCAGAAGAAACCCGAGCTTGTGCGGTCTCCCCTGGTGCCCCTGCCCATGCCCAAGAAGCTGCGGTTCAA CATCACCCCCGAGATCAAGAGCGACATCGAGAAGGCCAAGCAGAACCTCAGCATCATGATCCAGGACCTGGATATCACCGTGATGGTGTTCCACCATTTT GGAAAAGACTTCCCCAAGTCGGAGAAGCTAAGCCCAGATGCCTTCATCCAGATGGCTTTGCAGCTGGCCTACTACAGGATCTACGGACAGGCATGTGCCA CCTATGAAAGTGCCTCCCTGCGCATGTTTCACCTGGGCCGCACCGACACCATCCGCTCGGCTTCCATGGACTCACTCACCTTTGTCAAGGCCATGGATGA CTCCAGCGTCACGGAGCACCAGAAGGTGGAGCTGCTGCGGAAGGCCGTGCAGGCCCACCGAGGCTACACCGACCGGGCCATCCGCGGGGAGGCCTTTGAT CGACACCTGCTGGGCCTGAAGCTGCAGGCCATCGAGGACCTGGTGAGCATGCCCGACATCTTCATGGACACCTCCTACGCCATCGCCATGCACTTCCACC TCTCCACCAGCCAGGTCCCTGCCAAGACAGACTGTGTCATGTTCTTCGGGCCCGTGGTCCCCGACGGCTACGGTGTCTGCTATAACCCCATGGAGGCCCA CATCAACTTCTCCCTGTCGGCCTACAACAGCTGCGCGGAGACCAACGCCGCCCGCCTGGCGCATTACCTGGAGAAGGCGCTCCTGGACATGCGTGCCCTG CTGCAGAGCCACCCCCGGGCCAAGCTCTGAGCCCCTAGGACTCAGGCCTGCCAATGCCACAGCCAAGCCCACCCTGGGATGGGCCACCCACCAGGGCTCA GCTCCTTGGTTCCCTCTTCCTTGGTTCCCTCTTCCCTGGTCCCCCCAAATCTACTGAGCCACGGACCGCATCCTCCAGGGGGCCTGCAGGCCCCAGCCAA GTGCCTTCCGTGGGTCATCCCAGCACCTGCCAGGGCCCGACCTGGGGCTGAGTGCAGAGGCTGAGCAGGACGTTAGGCCCGGGCCCCTGGCACGTCCTCC ACCGGTGCCTCCTCTGGGGAAGGGAACCAGCCCTCCAGAGCAGGAGACTGGCAAGAGCTCTTTGTCTCACCAGCTCAGCCCCGGCCACTCCCTGCCAACT CCATGACCAGGCCACCATCTGTACCCTGCTTCCCAAACTCCCAGGACCTGGAGACAGGATTGTCTGGGGCCGAGGGGCCAGGGTGTGAGGTTTCACCTCC GTTGCGGCTGTGCTCCTGTGGATAACATTGCTAGCGAGCCGCCTCTGGTTCCACTCAGCTTGGTTCCTGCCCCCGCCCTGCTGTATGATATTAATGTGGA AGGTCATCAATAAAGGGGAATTGTGTTGGTGAA

refseq_CRAT.F2 (forward primer)
refseq_CRAT.R2 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

IDAPSPPHSQPPGHRCPPGARYRPRPRLASPPP RVPSRSPLGPEGGRGAASTGWRL*LSDLGPKALA RTHRPQPRRRRRGLCPVCGAERTACPRTGSEDV SLRCQDRGEASGLPEALLLDEGFQPLQGTPGCT ATAARAPSPAVPGPLPEGAAAHRE*GGVGPHQAA GG*VSGLRRCRGAPAEGAGASGQEDGELAV*VV AQDRLPPVPPACGHLLEPRRDATQAGLRGPAGS APICCQTH*GCVGFQGHD*QRDPARGVPGGEATV HEPVLSDLVLLPSAGPQAGHSQQLQQDQEASHA HHRGTQLPVF*AGCVPQ*RDTPHCGSDLCAAGE DLELIPTDQQGACGHPHLQPPQLLGQGIQHPHQR QGEPGFRALHPEETRACAVSPGAPAHAQEAAVQ HHPRDQERHREGQAEPQHHDPGPGYHRDGVPPF WKRLPQVGEAKPRCLHPDGFAAGLLQDLRTGMCH L*KCLPAHVSPGPHRHHPLGFHGLTHLCQGHG* LQRHGAPEGGAAAEGRAGPPRLHRPGHPRGGL* STPAGPEAAGHRGPGEHARHLHGHLLRHRHALPP LHQPGPCQDRLCHVLRARGPRRLRCLL*PHGGP HQLLPVGLQQLRGDQRRPPGALPGEGAPGHACP AAEPPPGQALSP*DSGLPMPQPSPPWDGPPTRAQ LLGSLFLGSLFPGPPKSTEPRTASSRGPAGPSQ VPSVGHPSTCQGPTWG*VQRLSRTLGPGPWHVL HRCLLWGREPALQSRRLARALCLTSSAPATPCQL HDQATICTLLPKLPGPGDRIVWGRGARV*GFTS VAAVLLWITLLASRLWFHSAWFLPPPCCMILMW KVINKGELCW*

Frame 1

SMPRPRPTANLPGTVARPGPATGPGPASRVLLP GCLPAARSAQRVGAGLPLPAGGCNSATLARRL* QGPTDPSRGGGGADFARCVGRSGLRVRGRAAKML AFAARTVVKPLGFLKPFSLMKASSRFKAHQDAL PRLPVPPLQQSLDHYLKALQPIVSEEEWAHTKQ LVDEFQASGGVGERLQKGLERRARKTENWLSEWW LKTAYLQYRQPVVIYSSPGVMLPKQDFVDLQGQ LRFAAKLIEGVLDFKVMIDNETLPVEYLGGKPL CMNQYYQILSSCRVPGPKQDTVSNFSKTKKPPTH ITVVHNYQFFELDVYHSDGTPLTADQIFVQLEK IWNSSLQTNKEPVGILTSNHRNSWAKAYNTLIK DKVNRDSVRSIQKKPELVRSPLVPLPMPKKLRFN ITPEIKSDIEKAKQNLSIMIQDLDITVMVFHHF GKDFPKSEKLSPDAFIQMALQLAYYRIYGQACA TYESASLRMFHLGRTDTIRSASMDSLTFVKAMDD SSVTEHQKVELLRKAVQAHRGYTDRAIRGEAFD RHLLGLKLQAIEDLVSMPDIFMDTSYAIAMHFH LSTSQVPAKTDCVMFFGPVVPDGYGVCYNPMEAH INFSLSAYNSCAETNAARLAHYLEKALLDMRAL LQSHPRAKL*APRTQACQCHSQAHPGMGHPPGL SSLVPSSLVPSSLVPPNLLSHGPHPPGGLQAPAK CLPWVIPAPARARPGAECRG*AGR*ARAPGTSS TGASSGEGNQPSRAGDWQELFVSPAQPRPLPAN SMTRPPSVPCFPNSQDLETGLSGAEGPGCEVSPP LRLCSCG*HC*RAASGSTQLGSCPRPAV*Y*CG RSSIKGNCVGE

Frame 2

RCPVPAPQPTSRAPLPARGPLPAQAPPRESSSP GAFPQPARPRGWARGCLYRLAAVTQRPWPEGSS KDPPTPAAAAAARTLPGVWGGADCVSADGQRRC *PSLPGPW*SLWAS*SPSP**RLPAASRHTRMHC HGCPCPLSSSPWTTT*RRCSPS*VRRSGPTPSS WWMSFRPQEV*GSACRRGWSVGPGRRRTGCLSG GSRPPTSSTASLWSSTRAQA*CYPSRTSWTCRVS SDLLPNSLRVCWISRS*LTTRPCPWSTWGGSHC A*TSTIRSCPPAECRAPSRTQSATSARPRSLPR TSPWYTTTSFLSWMCTTVTGHPSLRIRSLCSWRR SGTHPYRPTRSLWASSPPTTATPGPRHTTPSSK TR*TGIPCAPSRRNPSLCGLPWCPCPCPRSCGS TSPPRSRATSRRPSRTSAS*SRTWISP*WCSTIL EKTSPSRRS*AQMPSSRWLCSWPTTGSTDRHVP PMKVPPCACFTWAAPTPSARLPWTHSPLSRPWM TPASRSTRRWSCCGRPCRPTEATPTGPSAGRPLI DTCWA*SCRPSRTW*ACPTSSWTPPTPSPCTST SPPARSLPRQTVSCSSGPWSPTATVSAITPWRP TSTSPCRPTTAARRPTPPAWRITWRRRSWTCVPC CRATPGPSSEPLGLRPANATAKPTLGWATHQGS APWFPLPWFPLPWSPQIY*ATDRILQGACRPQP SAFRGSSQHLPGPDLGLSAEAEQDVRPGPLARPP PVPPLGKGTSPPEQETGKSSLSHQLSPGHSLPT P*PGHHLYPASQTPRTWRQDCLGPRGQGVRFHL RCGCAPVDNIASEPPLVPLSLVPAPALLYDINVE GHQ*RGIVLV

Sequence NM_004003

CTTCTAGAGTCCAGGAGCTTCCCCTGCCACTGCCACAACCCCCGATTCCACCACAGCCATCGCCACTGCCACCTGCCTCCCGCTGAATCTTGGCTCTTGT CTTCTCTTTCCAGCCCAGGTGAAGCCTCTGGGCTTCCTGAAGCCCTTCTCCTTGATGAAGGCTTCCAGCCGCTTCAAGGCACACCAGGATGCACTGCCAC GGCTGCCCGTGCCCCCTCTCCAGCAGTCCCTGGACCACTACCTGAAGGCGCTGCAGCCCATCGTGAGTGAGGAGGAGTGGGCCCACACCAAGCAGCTGGT GGATGAGTTTCAGGCCTCAGGAGGTGTAGGGGAGCGCCTGCAGAAGGGGCTGGAGCGTCGGGCCAGGAAGACGGAGAACTGGCTGTCTGAGTGGTGGCTC AAGACCGCCTACCTCCAGTACCGCCAGCCTGTGGTCATCTACTCGAGCCCAGGCGTGATGCTACCCAAGCAGGACTTCGTGGACCTGCAGGGTCAGCTCC GATTTGCTGCCAAACTCATTGAGGGTGTGTTGGATTTCAAGGTCATGATTGACAACGAGACCCTGCCCGTGGAGTACCTGGGGGGGAAGCCACTGTGCAT GAACCAGTACTATCAGATCTTGTCCTCCTGCCGAGTGCCGGGCCCCAAGCAGGACACAGTCAGCAACTTCAGCAAGACCAAGAAGCCTCCCACGCACATC ACCGTGGTACACAACTACCAGTTTTTTGAGCTGGATGTGTACCACAGTGACGGGACACCCCTCACTGCGGATCAGATCTTTGTGCAGCTGGAGAAGATCT GGAACTCATCCCTACAGACCAACAAGGAGCCTGTGGGCATCCTCACCTCCAACCACCGCAACTCCTGGGCCAAGGCATACAACACCCTCATCAAAGACAA GGTGAACCGGGATTCCGTGCGCTCCATCCAGAAGAGCATCTTCACCGTGTGCCTAGATGCAACCATGCCCAGGGTCTCAGAAGACGTGTACCGCAGCCAC GTGGCAGGCCAGATGCTGCATGGGGGCGGCAGCAGGCTCAACAGCGGCAACCGCTGGTTCGACAAGACGCTGCAGTTCATCGTGGCAGAAGATGGCTCCT GTGGGCTTGTGTACGAGCATGCTGCAGCGGAGGGGCCCCCTATTGTCACCCTTCTGGACTATGTCATCGAGTACACGAAGAAACCCGAGCTTGTGCGGTC TCCCCTGGTGCCCCTGCCCATGCCCAAGAAGCTGCGGTTCAACATCACCCCCGAGATCAAGAGCGACATCGAGAAGGCCAAGCAGAACCTCAGCATCATG ATCCAGGACCTGGATATCACCGTGATGGTGTTCCACCATTTTGGAAAAGACTTCCCCAAGTCGGAGAAGCTAAGCCCAGATGCCTTCATCCAGATGGCTT TGCAGCTGGCCTACTACAGGATCTACGGACAGGCATGTGCCACCTATGAAAGTGCCTCCCTGCGCATGTTTCACCTGGGCCGCACCGACACCATCCGCTC GGCTTCCATGGACTCACTCACCTTTGTCAAGGCCATGGATGACTCCAGCGTCACGGAGCACCAGAAGGTGGAGCTGCTGCGGAAGGCCGTGCAGGCCCAC CGAGGCTACACCGACCGGGCCATCCGCGGGGAGGCCTTTGATCGACACCTGCTGGGCCTGAAGCTGCAGGCCATCGAGGACCTGGTGAGCATGCCCGACA TCTTCATGGACACCTCCTACGCCATCGCCATGCACTTCCACCTCTCCACCAGCCAGGTCCCTGCCAAGACAGACTGTGTCATGTTCTTCGGGCCCGTGGT CCCCGACGGCTACGGTGTCTGCTATAACCCCATGGAGGCCCACATCAACTTCTCCCTGTCGGCCTACAACAGCTGCGCGGAGACCAACGCCGCCCGCCTG GCGCATTACCTGGAGAAGGCGCTCCTGGACATGCGTGCCCTGCTGCAGAGCCACCCCCGGGCCAAGCTCTGAGCCCCTAGGACTCAGGCCTGCCAATGCC ACAGCCAAGCCCACCCTGGGATGGGCCACCCACCAGGGCTCAGCTCCTTGGTTCCCTCTTCCTTGGTTCCCTCTTCCCTGGTCCCCCCAAATCTACTGAG CCACGGACCGCATCCTCCAGGGGGCCTGCAGGCCCCAGCCAAGTGCCTTCCGTGGGTCATCCCAGCACCTGCCAGGGCCCGACCTGGGGCTGAGTGCAGA GGCTGAGCAGGACGTTAGGCCCGGGCCCCTGGCACGTCCTCCACCGGTGCCTCCTCTGGGGAAGGGAACCAGCCCTCCAGAGCAGGAGACTGGCAAGAGC TCTTTGTCTCACCAGCTCAGCCCCGGCCACTCCCTGCCAACTCCATGACCAGGCCACCATCTGTACCCTGCTTCCCAAACTCCCAGGACCTGGAGACAGG ATTGTCTGGGGCCGAGGGGCCAGGGTGTGAGGTTTCACCTCCGTTGCGGCTGTGCTCCTGTGGATAACATTGCTAGCGAGCCGCCTCTGGTTCCACTCAG CTTGGTTCCTGCCCCCGCCCTGCTGTATGATATTAATGTGGAAGGTCATCAATAAAGGGGAATTGTGTTGGTGAA

refseq_CRAT.F2 (forward primer)
refseq_CRAT.R2 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

LLESRSFPCHCHNPRFHHSHRHCHLPPAESWLL SSLSSPGEASGLPEALLLDEGFQPLQGTPGCTAT AARAPSPAVPGPLPEGAAAHRE*GGVGPHQAAG G*VSGLRRCRGAPAEGAGASGQEDGELAV*VVA QDRLPPVPPACGHLLEPRRDATQAGLRGPAGSAP ICCQTH*GCVGFQGHD*QRDPARGVPGGEATVH EPVLSDLVLLPSAGPQAGHSQQLQQDQEASHAH HRGTQLPVF*AGCVPQ*RDTPHCGSDLCAAGEDL ELIPTDQQGACGHPHLQPPQLLGQGIQHPHQRQ GEPGFRALHPEEHLHRVPRCNHAQGLRRRVPQP RGRPDAAWGRQQAQQRQPLVRQDAAVHRGRRWLL WACVRACCSGGAPYCHPSGLCHRVHEETRACAV SPGAPAHAQEAAVQHHPRDQERHREGQAEPQHH DPGPGYHRDGVPPFWKRLPQVGEAKPRCLHPDGF AAGLLQDLRTGMCHL*KCLPAHVSPGPHRHHPL GFHGLTHLCQGHG*LQRHGAPEGGAAAEGRAGP PRLHRPGHPRGGL*STPAGPEAAGHRGPGEHARH LHGHLLRHRHALPPLHQPGPCQDRLCHVLRARG PRRLRCLL*PHGGPHQLLPVGLQQLRGDQRRPP GALPGEGAPGHACPAAEPPPGQALSP*DSGLPMP QPSPPWDGPPTRAQLLGSLFLGSLFPGPPKSTE PRTASSRGPAGPSQVPSVGHPSTCQGPTWG*VQ RLSRTLGPGPWHVLHRCLLWGREPALQSRRLARA LCLTSSAPATPCQLHDQATICTLLPKLPGPGDR IVWGRGARV*GFTSVAAVLLWITLLASRLWFHS AWFLPPPCCMILMWKVINKGELCW*

Frame 1

F*SPGASPATATTPDSTTAIATATCLPLNLGSC LLFPAQVKPLGFLKPFSLMKASSRFKAHQDALP RLPVPPLQQSLDHYLKALQPIVSEEEWAHTKQLV DEFQASGGVGERLQKGLERRARKTENWLSEWWL KTAYLQYRQPVVIYSSPGVMLPKQDFVDLQGQL RFAAKLIEGVLDFKVMIDNETLPVEYLGGKPLCM NQYYQILSSCRVPGPKQDTVSNFSKTKKPPTHI TVVHNYQFFELDVYHSDGTPLTADQIFVQLEKI WNSSLQTNKEPVGILTSNHRNSWAKAYNTLIKDK VNRDSVRSIQKSIFTVCLDATMPRVSEDVYRSH VAGQMLHGGGSRLNSGNRWFDKTLQFIVAEDGS CGLVYEHAAAEGPPIVTLLDYVIEYTKKPELVRS PLVPLPMPKKLRFNITPEIKSDIEKAKQNLSIM IQDLDITVMVFHHFGKDFPKSEKLSPDAFIQMA LQLAYYRIYGQACATYESASLRMFHLGRTDTIRS ASMDSLTFVKAMDDSSVTEHQKVELLRKAVQAH RGYTDRAIRGEAFDRHLLGLKLQAIEDLVSMPD IFMDTSYAIAMHFHLSTSQVPAKTDCVMFFGPVV PDGYGVCYNPMEAHINFSLSAYNSCAETNAARL AHYLEKALLDMRALLQSHPRAKL*APRTQACQC HSQAHPGMGHPPGLSSLVPSSLVPSSLVPPNLLS HGPHPPGGLQAPAKCLPWVIPAPARARPGAECR G*AGR*ARAPGTSSTGASSGEGNQPSRAGDWQE LFVSPAQPRPLPANSMTRPPSVPCFPNSQDLETG LSGAEGPGCEVSPPLRLCSCG*HC*RAASGSTQ LGSCPRPAV*Y*CGRSSIKGNCVGE

Frame 2

SRVQELPLPLPQPPIPPQPSPLPPASR*ILALV FSFQPR*SLWAS*SPSP**RLPAASRHTRMHCH GCPCPLSSSPWTTT*RRCSPS*VRRSGPTPSSW WMSFRPQEV*GSACRRGWSVGPGRRRTGCLSGGS RPPTSSTASLWSSTRAQA*CYPSRTSWTCRVSS DLLPNSLRVCWISRS*LTTRPCPWSTWGGSHCA *TSTIRSCPPAECRAPSRTQSATSARPRSLPRTS PWYTTTSFLSWMCTTVTGHPSLRIRSLCSWRRS GTHPYRPTRSLWASSPPTTATPGPRHTTPSSKT R*TGIPCAPSRRASSPCA*MQPCPGSQKTCTAAT WQARCCMGAAAGSTAATAGSTRRCSSSWQKMAP VGLCTSMLQRRGPLLSPFWTMSSSTRRNPSLCG LPWCPCPCPRSCGSTSPPRSRATSRRPSRTSAS* SRTWISP*WCSTILEKTSPSRRS*AQMPSSRWL CSWPTTGSTDRHVPPMKVPPCACFTWAAPTPSA RLPWTHSPLSRPWMTPASRSTRRWSCCGRPCRPT EATPTGPSAGRPLIDTCWA*SCRPSRTW*ACPT SSWTPPTPSPCTSTSPPARSLPRQTVSCSSGPW SPTATVSAITPWRPTSTSPCRPTTAARRPTPPAW RITWRRRSWTCVPCCRATPGPSSEPLGLRPANA TAKPTLGWATHQGSAPWFPLPWFPLPWSPQIY* ATDRILQGACRPQPSAFRGSSQHLPGPDLGLSAE AEQDVRPGPLARPPPVPPLGKGTSPPEQETGKS SLSHQLSPGHSLPTP*PGHHLYPASQTPRTWRQ DCLGPRGQGVRFHLRCGCAPVDNIASEPPLVPLS LVPAPALLYDINVEGHQ*RGIVLV

Sequence NM_000755

ATCGATGCCCCGTCCCCGCCCCACAGCCAACCTCCCGGGCACCGTTGCCCGCCCGGGGCCCGCTACCGGCCCAGGCCCCGCCTCGCGAGTCCTCCTCCCC GGGTGCCTTCCCGCAGCCCGCTCGGCCCAGAGGGTGGGCGCGGGGCTGCCTCTACCGGCTGGCGGCTGTAACTCAGCGACCTTGGCCCGAAGGCTCTAGC AAGGACCCACCGACCCCAGCCGCGGCGGCGGCGGCGCGGACTTTGCCCGGTGTGTGGGGCGGAGCGGACTGCGTGTCCGCGGACGGGCAGCGAAGATGTT AGCCTTCGCTGCCAGGACCGTGGTGAAGCCTCTGGGCTTCCTGAAGCCCTTCTCCTTGATGAAGGCTTCCAGCCGCTTCAAGGCACACCAGGATGCACTG CCACGGCTGCCCGTGCCCCCTCTCCAGCAGTCCCTGGACCACTACCTGAAGGCGCTGCAGCCCATCGTGAGTGAGGAGGAGTGGGCCCACACCAAGCAGC TGGTGGATGAGTTTCAGGCCTCAGGAGGTGTAGGGGAGCGCCTGCAGAAGGGGCTGGAGCGTCGGGCCAGGAAGACGGAGAACTGGCTGTCTGAGTGGTG GCTCAAGACCGCCTACCTCCAGTACCGCCAGCCTGTGGTCATCTACTCGAGCCCAGGCGTGATGCTACCCAAGCAGGACTTCGTGGACCTGCAGGGTCAG CTCCGATTTGCTGCCAAACTCATTGAGGGTGTGTTGGATTTCAAGGTCATGATTGACAACGAGACCCTGCCCGTGGAGTACCTGGGGGGGAAGCCACTGT GCATGAACCAGTACTATCAGATCTTGTCCTCCTGCCGAGTGCCGGGCCCCAAGCAGGACACAGTCAGCAACTTCAGCAAGACCAAGAAGCCTCCCACGCA CATCACCGTGGTACACAACTACCAGTTTTTTGAGCTGGATGTGTACCACAGTGACGGGACACCCCTCACTGCGGATCAGATCTTTGTGCAGCTGGAGAAG ATCTGGAACTCATCCCTACAGACCAACAAGGAGCCTGTGGGCATCCTCACCTCCAACCACCGCAACTCCTGGGCCAAGGCATACAACACCCTCATCAAAG ACAAGGTGAACCGGGATTCCGTGCGCTCCATCCAGAAGAGCATCTTCACCGTGTGCCTAGATGCAACCATGCCCAGGGTCTCAGAAGACGTGTACCGCAG CCACGTGGCAGGCCAGATGCTGCATGGGGGCGGCAGCAGGCTCAACAGCGGCAACCGCTGGTTCGACAAGACGCTGCAGTTCATCGTGGCAGAAGATGGC TCCTGTGGGCTTGTGTACGAGCATGCTGCAGCGGAGGGGCCCCCTATTGTCACCCTTCTGGACTATGTCATCGAGTACACGAAGAAACCCGAGCTTGTGC GGTCTCCCCTGGTGCCCCTGCCCATGCCCAAGAAGCTGCGGTTCAACATCACCCCCGAGATCAAGAGCGACATCGAGAAGGCCAAGCAGAACCTCAGCAT CATGATCCAGGACCTGGATATCACCGTGATGGTGTTCCACCATTTTGGAAAAGACTTCCCCAAGTCGGAGAAGCTAAGCCCAGATGCCTTCATCCAGATG GCTTTGCAGCTGGCCTACTACAGGATCTACGGACAGGCATGTGCCACCTATGAAAGTGCCTCCCTGCGCATGTTTCACCTGGGCCGCACCGACACCATCC GCTCGGCTTCCATGGACTCACTCACCTTTGTCAAGGCCATGGATGACTCCAGCGTCACGGAGCACCAGAAGGTGGAGCTGCTGCGGAAGGCCGTGCAGGC CCACCGAGGCTACACCGACCGGGCCATCCGCGGGGAGGCCTTTGATCGACACCTGCTGGGCCTGAAGCTGCAGGCCATCGAGGACCTGGTGAGCATGCCC GACATCTTCATGGACACCTCCTACGCCATCGCCATGCACTTCCACCTCTCCACCAGCCAGGTCCCTGCCAAGACAGACTGTGTCATGTTCTTCGGGCCCG TGGTCCCCGACGGCTACGGTGTCTGCTATAACCCCATGGAGGCCCACATCAACTTCTCCCTGTCGGCCTACAACAGCTGCGCGGAGACCAACGCCGCCCG CCTGGCGCATTACCTGGAGAAGGCGCTCCTGGACATGCGTGCCCTGCTGCAGAGCCACCCCCGGGCCAAGCTCTGAGCCCCTAGGACTCAGGCCTGCCAA TGCCACAGCCAAGCCCACCCTGGGATGGGCCACCCACCAGGGCTCAGCTCCTTGGTTCCCTCTTCCTTGGTTCCCTCTTCCCTGGTCCCCCCAAATCTAC TGAGCCACGGACCGCATCCTCCAGGGGGCCTGCAGGCCCCAGCCAAGTGCCTTCCGTGGGTCATCCCAGCACCTGCCAGGGCCCGACCTGGGGCTGAGTG CAGAGGCTGAGCAGGACGTTAGGCCCGGGCCCCTGGCACGTCCTCCACCGGTGCCTCCTCTGGGGAAGGGAACCAGCCCTCCAGAGCAGGAGACTGGCAA GAGCTCTTTGTCTCACCAGCTCAGCCCCGGCCACTCCCTGCCAACTCCATGACCAGGCCACCATCTGTACCCTGCTTCCCAAACTCCCAGGACCTGGAGA CAGGATTGTCTGGGGCCGAGGGGCCAGGGTGTGAGGTTTCACCTCCGTTGCGGCTGTGCTCCTGTGGATAACATTGCTAGCGAGCCGCCTCTGGTTCCAC TCAGCTTGGTTCCTGCCCCCGCCCTGCTGTATGATATTAATGTGGAAGGTCATCAATAAAGGGGAATTGTGTTGGTGAA

refseq_CRAT.F2 (forward primer)
refseq_CRAT.R2 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

IDAPSPPHSQPPGHRCPPGARYRPRPRLASPPP RVPSRSPLGPEGGRGAASTGWRL*LSDLGPKALA RTHRPQPRRRRRGLCPVCGAERTACPRTGSEDV SLRCQDRGEASGLPEALLLDEGFQPLQGTPGCT ATAARAPSPAVPGPLPEGAAAHRE*GGVGPHQAA GG*VSGLRRCRGAPAEGAGASGQEDGELAV*VV AQDRLPPVPPACGHLLEPRRDATQAGLRGPAGS APICCQTH*GCVGFQGHD*QRDPARGVPGGEATV HEPVLSDLVLLPSAGPQAGHSQQLQQDQEASHA HHRGTQLPVF*AGCVPQ*RDTPHCGSDLCAAGE DLELIPTDQQGACGHPHLQPPQLLGQGIQHPHQR QGEPGFRALHPEEHLHRVPRCNHAQGLRRRVPQ PRGRPDAAWGRQQAQQRQPLVRQDAAVHRGRRW LLWACVRACCSGGAPYCHPSGLCHRVHEETRACA VSPGAPAHAQEAAVQHHPRDQERHREGQAEPQH HDPGPGYHRDGVPPFWKRLPQVGEAKPRCLHPD GFAAGLLQDLRTGMCHL*KCLPAHVSPGPHRHHP LGFHGLTHLCQGHG*LQRHGAPEGGAAAEGRAG PPRLHRPGHPRGGL*STPAGPEAAGHRGPGEHA RHLHGHLLRHRHALPPLHQPGPCQDRLCHVLRAR GPRRLRCLL*PHGGPHQLLPVGLQQLRGDQRRP PGALPGEGAPGHACPAAEPPPGQALSP*DSGLP MPQPSPPWDGPPTRAQLLGSLFLGSLFPGPPKST EPRTASSRGPAGPSQVPSVGHPSTCQGPTWG*V QRLSRTLGPGPWHVLHRCLLWGREPALQSRRLA RALCLTSSAPATPCQLHDQATICTLLPKLPGPGD RIVWGRGARV*GFTSVAAVLLWITLLASRLWFH SAWFLPPPCCMILMWKVINKGELCW*

Frame 1

SMPRPRPTANLPGTVARPGPATGPGPASRVLLP GCLPAARSAQRVGAGLPLPAGGCNSATLARRL* QGPTDPSRGGGGADFARCVGRSGLRVRGRAAKML AFAARTVVKPLGFLKPFSLMKASSRFKAHQDAL PRLPVPPLQQSLDHYLKALQPIVSEEEWAHTKQ LVDEFQASGGVGERLQKGLERRARKTENWLSEWW LKTAYLQYRQPVVIYSSPGVMLPKQDFVDLQGQ LRFAAKLIEGVLDFKVMIDNETLPVEYLGGKPL CMNQYYQILSSCRVPGPKQDTVSNFSKTKKPPTH ITVVHNYQFFELDVYHSDGTPLTADQIFVQLEK IWNSSLQTNKEPVGILTSNHRNSWAKAYNTLIK DKVNRDSVRSIQKSIFTVCLDATMPRVSEDVYRS HVAGQMLHGGGSRLNSGNRWFDKTLQFIVAEDG SCGLVYEHAAAEGPPIVTLLDYVIEYTKKPELV RSPLVPLPMPKKLRFNITPEIKSDIEKAKQNLSI MIQDLDITVMVFHHFGKDFPKSEKLSPDAFIQM ALQLAYYRIYGQACATYESASLRMFHLGRTDTI RSASMDSLTFVKAMDDSSVTEHQKVELLRKAVQA HRGYTDRAIRGEAFDRHLLGLKLQAIEDLVSMP DIFMDTSYAIAMHFHLSTSQVPAKTDCVMFFGP VVPDGYGVCYNPMEAHINFSLSAYNSCAETNAAR LAHYLEKALLDMRALLQSHPRAKL*APRTQACQ CHSQAHPGMGHPPGLSSLVPSSLVPSSLVPPNL LSHGPHPPGGLQAPAKCLPWVIPAPARARPGAEC RG*AGR*ARAPGTSSTGASSGEGNQPSRAGDWQ ELFVSPAQPRPLPANSMTRPPSVPCFPNSQDLE TGLSGAEGPGCEVSPPLRLCSCG*HC*RAASGST QLGSCPRPAV*Y*CGRSSIKGNCVGE

Frame 2

RCPVPAPQPTSRAPLPARGPLPAQAPPRESSSP GAFPQPARPRGWARGCLYRLAAVTQRPWPEGSS KDPPTPAAAAAARTLPGVWGGADCVSADGQRRC *PSLPGPW*SLWAS*SPSP**RLPAASRHTRMHC HGCPCPLSSSPWTTT*RRCSPS*VRRSGPTPSS WWMSFRPQEV*GSACRRGWSVGPGRRRTGCLSG GSRPPTSSTASLWSSTRAQA*CYPSRTSWTCRVS SDLLPNSLRVCWISRS*LTTRPCPWSTWGGSHC A*TSTIRSCPPAECRAPSRTQSATSARPRSLPR TSPWYTTTSFLSWMCTTVTGHPSLRIRSLCSWRR SGTHPYRPTRSLWASSPPTTATPGPRHTTPSSK TR*TGIPCAPSRRASSPCA*MQPCPGSQKTCTA ATWQARCCMGAAAGSTAATAGSTRRCSSSWQKMA PVGLCTSMLQRRGPLLSPFWTMSSSTRRNPSLC GLPWCPCPCPRSCGSTSPPRSRATSRRPSRTSA S*SRTWISP*WCSTILEKTSPSRRS*AQMPSSRW LCSWPTTGSTDRHVPPMKVPPCACFTWAAPTPS ARLPWTHSPLSRPWMTPASRSTRRWSCCGRPCR PTEATPTGPSAGRPLIDTCWA*SCRPSRTW*ACP TSSWTPPTPSPCTSTSPPARSLPRQTVSCSSGP WSPTATVSAITPWRPTSTSPCRPTTAARRPTPP AWRITWRRRSWTCVPCCRATPGPSSEPLGLRPAN ATAKPTLGWATHQGSAPWFPLPWFPLPWSPQIY *ATDRILQGACRPQPSAFRGSSQHLPGPDLGLS AEAEQDVRPGPLARPPPVPPLGKGTSPPEQETGK SSLSHQLSPGHSLPTP*PGHHLYPASQTPRTWR QDCLGPRGQGVRFHLRCGCAPVDNIASEPPLVP LSLVPAPALLYDINVEGHQ*RGIVLV