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Sequence NM_031423
GGAATGGGGCGGGACTTCCAGTAGGAGGCGGCAAGTTTGAAAAGTGATGACGGTTGACGTTTGCTGATTTTTGACTTTGCTTGTAGCTGCTCCCCGAACTCGCCGTCTTCCTGTCGGCGGCCGGCACTGTAGATTAACAGGAAACTTCCAAGATGGAAACTTTGTCTTTCCCCAGATATAATGTAGCTGAGATTGTGATT
CATATTCGCAATAAGATCTTAACAGGAGCTGATGGTAAAAACCTCACCAAGAATGATCTTTATCCAAATCCAAAGCCTGAAGTCTTGCACATGATCTACA
TGAGAGCCTTACAAATAGTATATGGAATTCGACTGGAACATTTTTACATGATGCCAGTGAACTCTGAAGTCATGTATCCACATTTAATGGAAGGCTTCTT
ACCATTCAGCAATTTAGTTACTCATCTGGACTCATTTTTGCCTATCTGCCGGGTGAATGACTTTGAGACTGCTGATATTCTATGTCCAAAAGCAAAACGG
ACAAGTCGGTTTTTAAGTGGCATTATCAACTTTATTCACTTCAGAGAAGCATGCCGTGAAACGTATATGGAATTTCTTTGGCAATATAAATCCTCTGCGG
ACAAAATGCAACAGTTAAACGCCGCACACCAGGAGGCATTAATGAAACTGGAGAGACTTGATTCTGTTCCAGTTGAAGAGCAAGAAGAGTTCAAGCAGCT
TTCAGATGGAATTCAGGAGCTACAACAATCACTAAATCAGGATTTTCATCAAAAAACGATAGTGCTGCAAGAGGGAAATTCCCAAAAGAAGTCAAATATT
TCAGAGAAAACCAAGCGTTTGAATGAACTAAAATTGTCGGTGGTTTCTTTGAAAGAAATACAAGAGAGTTTGAAAACAAAAATTGTGGATTCTCCAGAGA
AGTTAAAGAATTATAAAGAAAAAATGAAAGATACGGTCCAGAAGCTTAAAAATGCCAGACAAGAAGTGGTGGAGAAATATGAAATCTATGGAGACTCAGT
TGACTGCCTGCCTTCATGTCAGTTGGAAGTGCAGTTATATCAAAAGAAAATACAGGACCTTTCAGATAATAGGGAAAAATTAGCCAGTATCTTAAAGGAG
AGCCTGAACTTGGAGGACCAAATTGAGAGTGATGAGTCAGAACTGAAGAAATTGAAGACTGAAGAAAATTCGTTCAAAAGACTGATGATTGTGAAGAAGG
AAAAACTTGCCACAGCACAATTCAAAATAAATAAGAAGCATGAAGATGTTAAGCAATACAAACGCACAGTAATTGAGGATTGCAATAAAGTTCAAGAAAA
AAGAGGTGCTGTCTATGAACGAGTAACCACAATTAATCAAGAAATCCAAAAAATTAAACTTGGAATTCAACAACTAAAAGATGCTGCTGAAAGGGAGAAA
CTGAAGTCCCAGGAAATATTTCTAAACTTGAAAACTGCTTTGGAGAAATACCACGACGGTATTGAAAAGGCAGCAGAGGACTCCTATGCTAAGATAGATG
AGAAGACAGCTGAACTGAAGAGGAAGATGTTCAAAATGTCAACCTGATTAACAAAATTACATGTCTTTTTGTAAATGGCTTGCCATCTTTTAATTTTCTA
TTTAGAAAGAAAAGTTGAAGCGAATGGAAGTATCAGAAGTACCAAATAATGTTGGCTTCATCAGTTTTTATACACTCTCATAAGTAGTTAATAAGATGAA
TTTAATGTAGGCTTTTATTAATTTATAATTAAAATAACTTGTGCAGCTATTCATGTCTCTACTCTGCCCCTTGTTGTAAATAGTTTGAGTAAAACAAAAC
TAGTTACCTTTGAAATATATATATTTTTTTCTGTTA
- refseq_CDCA1.F1 (forward primer)
- refseq_CDCA1.R1 (reverse primer)
- Alternative donor
Frame 0
GMGRDFQ*EAASLKSDDG*RLLIFDFACSCSPNSPSSCRRPAL*INRKLPRWKLCLSPDIM*LRL*F
IFAIRS*QELMVKTSPRMIFIQIQSLKSCT*ST
*EPYK*YMEFDWNIFT*CQ*TLKSCIHI*WKAS
YHSAI*LLIWTHFCLSAG*MTLRLLIFYVQKQNG
QVGF*VALSTLFTSEKHAVKRIWNFFGNINPLR
TKCNS*TPHTRRH**NWRDLILFQLKSKKSSSS
FQMEFRSYNNH*IRIFIKKR*CCKREIPKRSQIF
QRKPSV*MN*NCRWFL*KKYKRV*KQKLWILQR
S*RIIKKK*KIRSRSLKMPDKKWWRNMKSMETQ
LTACLHVSWKCSYIKRKYRTFQIIGKN*PVS*RR
A*TWRTKLRVMSQN*RN*RLKKIRSKD**L*RR
KNLPQHNSK*IRSMKMLSNTNAQ*LRIAIKFKK
KEVLSMNE*PQLIKKSKKLNLEFNN*KMLLKGRN
*SPRKYF*T*KLLWRNTTTVLKRQQRTPMLR*M
RRQLN*RGRCSKCQPD*QNYMSFCKWLAIF*FS
I*KEKLKRMEVSEVPNNVGFISFYTLS*VVNKMN
LM*AFINL*LK*LVQLFMSLLCPLL*IV*VKQN
*LPLKYIYFFLL
Frame 1
EWGGTSSRRRQV*KVMTVDVC*FLTLLVAAPRTRRLPVGGRHCRLTGNFQDGNFVFPQI*CS*DCD
SYSQ*DLNRS*W*KPHQE*SLSKSKA*SLAHDLH
ESLTNSIWNSTGTFLHDASEL*SHVSTFNGRLL
TIQQFSYSSGLIFAYLPGE*L*DC*YSMSKSKT
DKSVFKWHYQLYSLQRSMP*NVYGISLAI*ILCG
QNATVKRRTPGGINETGET*FCSS*RARRVQAA
FRWNSGATTITKSGFSSKNDSAARGKFPKEVKY
FRENQAFE*TKIVGGFFERNTREFENKNCGFSRE
VKEL*RKNERYGPEA*KCQTRSGGEI*NLWRLS
*LPAFMSVGSAVISKENTGPFR**GKISQYLKG
EPELGGPN*E**VRTEEIED*RKFVQKTDDCEEG
KTCHSTIQNK*EA*RC*AIQTHSN*GLQ*SSRK
KRCCL*TSNHN*SRNPKN*TWNSTTKRCC*KGE
TEVPGNISKLENCFGEIPRRY*KGSRGLLC*DR*
EDS*TEEEDVQNVNLINKITCLFVNGLPSFNFL
FRKKS*SEWKYQKYQIMLASSVFIHSHK*LIR*
I*CRLLLIYN*NNLCSYSCLYSAPCCK*FE*NKT
SYL*NIYIFFC
Frame 2
NGAGLPVGGGKFEK**RLTFADF*LCL*LLPELAVFLSAAGTVD*QETSKMETLSFPRYNVAEIVI
HIRNKILTGADGKNLTKNDLYPNPKPEVLHMIY
MRALQIVYGIRLEHFYMMPVNSEVMYPHLMEGFL
PFSNLVTHLDSFLPICRVNDFETADILCPKAKR
TSRFLSGIINFIHFREACRETYMEFLWQYKSSA
DKMQQLNAAHQEALMKLERLDSVPVEEQEEFKQL
SDGIQELQQSLNQDFHQKTIVLQEGNSQKKSNI
SEKTKRLNELKLSVVSLKEIQESLKTKIVDSPE
KLKNYKEKMKDTVQKLKNARQEVVEKYEIYGDSV
DCLPSCQLEVQLYQKKIQDLSDNREKLASILKE
SLNLEDQIESDESELKKLKTEENSFKRLMIVKK
EKLATAQFKINKKHEDVKQYKRTVIEDCNKVQEK
RGAVYERVTTINQEIQKIKLGIQQLKDAAEREK
LKSQEIFLNLKTALEKYHDGIEKAAEDSYAKID
EKTAELKRKMFKMST*LTKLHVFL*MACHLLIFY
LERKVEANGSIRSTK*CWLHQFLYTLISS**DE
FNVGFY*FIIKITCAAIHVSTLPLVVNSLSKTK
LVTFEIYIFFSV
Sequence NM_145697
GGAATGGGGCGGGACTTCCAGTAGGAGGCGGCAAGTTTGAAAAGTGATGACGGTTGACGTTTGCTGATTTTTGACTTTGCTTGTAGCTGCTCCCCGAACTCGCCGTCTTCCTGTCGGCGGCCGGCACTGTAGGTGAGCGCGAGAGGACGGAGGAAGGAAGCCTGCAGACAGACGCCTTCTCCATCCCAAGGCGCGGGCAG
GTGCCGGGACGCTGGGCCTGGCGGTGTTTTCGTCGTGCTCAGCGGTGGGAGGAGGCGGAAGAAACCAGAGCCTGGGAGATTAACAGGAAACTTCCAAGAT
GGAAACTTTGTCTTTCCCCAGATATAATGTAGCTGAGATTGTGATTCATATTCGCAATAAGATCTTAACAGGAGCTGATGGTAAAAACCTCACCAAGAAT
GATCTTTATCCAAATCCAAAGCCTGAAGTCTTGCACATGATCTACATGAGAGCCTTACAAATAGTATATGGAATTCGACTGGAACATTTTTACATGATGC
CAGTGAACTCTGAAGTCATGTATCCACATTTAATGGAAGGCTTCTTACCATTCAGCAATTTAGTTACTCATCTGGACTCATTTTTGCCTATCTGCCGGGT
GAATGACTTTGAGACTGCTGATATTCTATGTCCAAAAGCAAAACGGACAAGTCGGTTTTTAAGTGGCATTATCAACTTTATTCACTTCAGAGAAGCATGC
CGTGAAACGTATATGGAATTTCTTTGGCAATATAAATCCTCTGCGGACAAAATGCAACAGTTAAACGCCGCACACCAGGAGGCATTAATGAAACTGGAGA
GACTTGATTCTGTTCCAGTTGAAGAGCAAGAAGAGTTCAAGCAGCTTTCAGATGGAATTCAGGAGCTACAACAATCACTAAATCAGGATTTTCATCAAAA
AACGATAGTGCTGCAAGAGGGAAATTCCCAAAAGAAGTCAAATATTTCAGAGAAAACCAAGCGTTTGAATGAACTAAAATTGTCGGTGGTTTCTTTGAAA
GAAATACAAGAGAGTTTGAAAACAAAAATTGTGGATTCTCCAGAGAAGTTAAAGAATTATAAAGAAAAAATGAAAGATACGGTCCAGAAGCTTAAAAATG
CCAGACAAGAAGTGGTGGAGAAATATGAAATCTATGGAGACTCAGTTGACTGCCTGCCTTCATGTCAGTTGGAAGTGCAGTTATATCAAAAGAAAATACA
GGACCTTTCAGATAATAGGGAAAAATTAGCCAGTATCTTAAAGGAGAGCCTGAACTTGGAGGACCAAATTGAGAGTGATGAGTCAGAACTGAAGAAATTG
AAGACTGAAGAAAATTCGTTCAAAAGACTGATGATTGTGAAGAAGGAAAAACTTGCCACAGCACAATTCAAAATAAATAAGAAGCATGAAGATGTTAAGC
AATACAAACGCACAGTAATTGAGGATTGCAATAAAGTTCAAGAAAAAAGAGGTGCTGTCTATGAACGAGTAACCACAATTAATCAAGAAATCCAAAAAAT
TAAACTTGGAATTCAACAACTAAAAGATGCTGCTGAAAGGGAGAAACTGAAGTCCCAGGAAATATTTCTAAACTTGAAAACTGCTTTGGAGAAATACCAC
GACGGTATTGAAAAGGCAGCAGAGGACTCCTATGCTAAGATAGATGAGAAGACAGCTGAACTGAAGAGGAAGATGTTCAAAATGTCAACCTGATTAACAA
AATTACATGTCTTTTTGTAAATGGCTTGCCATCTTTTAATTTTCTATTTAGAAAGAAAAGTTGAAGCGAATGGAAGTATCAGAAGTACCAAATAATGTTG
GCTTCATCAGTTTTTATACACTCTCATAAGTAGTTAATAAGATGAATTTAATGTAGGCTTTTATTAATTTATAATTAAAATAACTTGTGCAGCTATTCAT
GTCTCTACTCTGCCCCTTGTTGTAAATAGTTTGAGTAAAACAAAACTAGTTACCTTTGAAATATATATATTTTTTTCTGTTA
- refseq_CDCA1.F1 (forward primer)
- refseq_CDCA1.R1 (reverse primer)
- Alternative donor
Frame 0
GMGRDFQ*EAASLKSDDG*RLLIFDFACSCSPNSPSSCRRPAL*VSARGRRKEACRQTPSPSQGAGR
CRDAGPGGVFVVLSGGRRRKKPEPGRLTGNFQD
GNFVFPQI*CS*DCDSYSQ*DLNRS*W*KPHQE
*SLSKSKA*SLAHDLHESLTNSIWNSTGTFLHDA
SEL*SHVSTFNGRLLTIQQFSYSSGLIFAYLPG
E*L*DC*YSMSKSKTDKSVFKWHYQLYSLQRSM
P*NVYGISLAI*ILCGQNATVKRRTPGGINETGE
T*FCSS*RARRVQAAFRWNSGATTITKSGFSSK
NDSAARGKFPKEVKYFRENQAFE*TKIVGGFFE
RNTREFENKNCGFSREVKEL*RKNERYGPEA*KC
QTRSGGEI*NLWRLS*LPAFMSVGSAVISKENT
GPFR**GKISQYLKGEPELGGPN*E**VRTEEI
ED*RKFVQKTDDCEEGKTCHSTIQNK*EA*RC*A
IQTHSN*GLQ*SSRKKRCCL*TSNHN*SRNPKN
*TWNSTTKRCC*KGETEVPGNISKLENCFGEIP
RRY*KGSRGLLC*DR*EDS*TEEEDVQNVNLINK
ITCLFVNGLPSFNFLFRKKS*SEWKYQKYQIML
ASSVFIHSHK*LIR*I*CRLLLIYN*NNLCSYS
CLYSAPCCK*FE*NKTSYL*NIYIFFC
Frame 1
EWGGTSSRRRQV*KVMTVDVC*FLTLLVAAPRTRRLPVGGRHCR*AREDGGRKPADRRLLHPKARA
GAGTLGLAVFSSCSAVGGGGRNQSLGD*QETSKM
ETLSFPRYNVAEIVIHIRNKILTGADGKNLTKN
DLYPNPKPEVLHMIYMRALQIVYGIRLEHFYMM
PVNSEVMYPHLMEGFLPFSNLVTHLDSFLPICRV
NDFETADILCPKAKRTSRFLSGIINFIHFREAC
RETYMEFLWQYKSSADKMQQLNAAHQEALMKLE
RLDSVPVEEQEEFKQLSDGIQELQQSLNQDFHQK
TIVLQEGNSQKKSNISEKTKRLNELKLSVVSLK
EIQESLKTKIVDSPEKLKNYKEKMKDTVQKLKN
ARQEVVEKYEIYGDSVDCLPSCQLEVQLYQKKIQ
DLSDNREKLASILKESLNLEDQIESDESELKKL
KTEENSFKRLMIVKKEKLATAQFKINKKHEDVK
QYKRTVIEDCNKVQEKRGAVYERVTTINQEIQKI
KLGIQQLKDAAEREKLKSQEIFLNLKTALEKYH
DGIEKAAEDSYAKIDEKTAELKRKMFKMST*LT
KLHVFL*MACHLLIFYLERKVEANGSIRSTK*CW
LHQFLYTLISS**DEFNVGFY*FIIKITCAAIH
VSTLPLVVNSLSKTKLVTFEIYIFFSV
Frame 2
NGAGLPVGGGKFEK**RLTFADF*LCL*LLPELAVFLSAAGTVGERERTEEGSLQTDAFSIPRRGQ
VPGRWAWRCFRRAQRWEEAEETRAWEINRKLPR
WKLCLSPDIM*LRL*FIFAIRS*QELMVKTSPRM
IFIQIQSLKSCT*ST*EPYK*YMEFDWNIFT*C
Q*TLKSCIHI*WKASYHSAI*LLIWTHFCLSAG
*MTLRLLIFYVQKQNGQVGF*VALSTLFTSEKHA
VKRIWNFFGNINPLRTKCNS*TPHTRRH**NWR
DLILFQLKSKKSSSSFQMEFRSYNNH*IRIFIK
KR*CCKREIPKRSQIFQRKPSV*MN*NCRWFL*K
KYKRV*KQKLWILQRS*RIIKKK*KIRSRSLKM
PDKKWWRNMKSMETQLTACLHVSWKCSYIKRKY
RTFQIIGKN*PVS*RRA*TWRTKLRVMSQN*RN*
RLKKIRSKD**L*RRKNLPQHNSK*IRSMKMLS
NTNAQ*LRIAIKFKKKEVLSMNE*PQLIKKSKK
LNLEFNN*KMLLKGRN*SPRKYF*T*KLLWRNTT
TVLKRQQRTPMLR*MRRQLN*RGRCSKCQPD*Q
NYMSFCKWLAIF*FSI*KEKLKRMEVSEVPNNV
GFISFYTLS*VVNKMNLM*AFINL*LK*LVQLFM
SLLCPLL*IV*VKQN*LPLKYIYFFLL
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