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Sequences

Sequence NM_001005766

AGAGGAGAGGCCAGCGGATTAAAGGGTTTCATGTTGCCACAGCCTCTCCCCCAGTCCCTGTCTCCGAGGCCTGCTTGCTAGGTGGGGCGACTCTTCTGTG
AGCTGCAGCTGTGGGGCAGAGAGAAGCCCTCCCCACCCAGGACTGAGAGGCTGGAGACCCCAGGCTCGCCAAGGCACGGAAGACAAAGGCCTGGGGCGTC
CTGGGGTCTGGGAGGAAGGCAGACAGAGCTCATGGTCTGTGGCTGCTCTGCCCTCCTTCCCCTCCCCAACCCCAGGCCCACCATGCCTGCAACTCCCAAC
TTCCTCGCAAACCCCAGCTCCAGCAGCCGCTGGATTCCCCTCCAGCCAATGCCCGTGGCCTGGGCCTTTGTGCAGAAGACCTCGGCCCTCCTGTGGCTGC
TGCTTCTAGGCACCTCCCTGTCCCCTGCGTGGGGACAGGCCAAGATTCCTCTGGAAACAGTGAAGCTATGGGCTGACACCTTCGGCGGGGACCTGTATAA
CACTGTGACCAAATACTCAGGCTCTCTCTTGCTGCAGAAGAAGTACAAGGATGTGGAGTCCAGTCTGAAGATCGAGGAGGTGGATGGCTTGGAGCTGGTG
AGGAAGTTCTCAGAGGACATGGAGAACATGCTGCGGAGGAAAGTCGAGGCGGTCCAGAATCTGGTGGAAGCTGCCGAGGAGGCCGACCTGAACCACGAAT
TCAATGAATCCCTGGTGTTCGACTATTACAACTCGGTCCTGATCAACGAGAGGGACGAGAAGGGCAACTTCGTGGAGCTGGGCGCCGAGTTCCTCCTGGA
GTCCAATGCTCACTTCAGCAACCTGCCGGTGAACACCTCCATCAGCAGCGTGCAGCTGCCCACCAACGTGTACAACAAAGACCCAGATATTTTAAATGGA
GTCTACATGTCTGAAGCCTTGAATGCTGTCTTCGTGGAGAACTTCCAGAGAGACCCAACGTTGACCTGGCAATATTTTGGCAGTGCAACTGGATTCTTCA
GGATCTATCCAGGTATAAAATGGACACCTGATGAGAATGGAGTCATTACTTTTGACTGCCGAAACCGCGGCTGGTACATTCAAGCTGCTACTTCTCCCAA
GGACATAGTGATTTTGGTGGACGTGAGCGGCAGTATGAAGGGGCTGAGGATGACTATTGCCAAGCACACCATCACCACCATCTTGGACACCCTGGGGGAG
AATGACTTCATTAATATCATAGCGTACAATGACTACGTCCATTACATCGAGCCTTGTTTTAAAGGGATCCTCGTCCAGGCGGACCGAGACAATCGAGAGC
ATTTCAAACTGCTGGTGGAGGAGTTGATGGTCAAAGGTGTGGGGGTCGTGGACCAAGCCCTGAGAGAAGCCTTCCAGATCCTGAAGCAGTTCCAAGAGGC
CAAGCAAGGAAGCCTCTGCAACCAGGCCATCATGCTCATCAGCGACGGCGCCGTGGAGGACTACGAGCCGGTGTTTGAGAAGTATAACTGGCCAGACTGT
AAGGTCCGAGTTTTCACTTACCTCATTGGGAGAGAAGTGTCTTTTGCTGACCGCATGAAGTGGATTGCATGCAACAACAAAGGCTACTACACGCAGATCT
CAACGCTGGCGGACACCCAGGAGAACGTGATGGAATACCTGCACGTGCTCAGCCGCCCCATGGTCATCAACCACGACCACGACATCATCTGGACAGAGGC
CTACATGGACAGCAAGCTCCTCAGCTCGCAGGCTCAGAGCCTGACACTGCTCACCACTGTGGCCATGCCAGTCTTCAGCAAGAAGAACGAAACGCGATCC
CATGGCATTCTCCTGGGTGTGGTGGGCTCAGATGTGGCCCTGAGAGAGCTGATGAAGCTGGCGCCCCGGTACAAGCTTGGAGTGCACGGATACGCCTTTC
TGAACACCAACAATGGCTACATCCTCTCCCATCCCGACCTCCGGCCCCTGTACAGAGAGGGGAAGAAACTAAAACCCAAACCTAACTACAACAGTGTGGA
TCTCTCCGAAGTGGAGTGGGAAGACCAGGCTGAATCTCTGAGAACAGCCATGATCAATAGGGAAACAGGTACTCTCTCGATGGATGTGAAGGTTCCGATG
GATAAAGGGAAGCGAGTTCTTTTCCTGACCAATGACTACTTCTTCACGGACATCAGCGACACCCCTTTCAGTTTGGGGGTGGTGCTGTCCCGGGGCCACG
GAGAATACATCCTTCTGGGGAACACGTCTGTGGAAGAAGGCCTGCATGACTTGCTTCACCCAGACCTGGCCCTGGCCGGTGACTGGATCTACTGCATCAC
AGATATTGACCCAGACCACCGGAAGCTCAGCCAGCTAGAGGCCATGATCCGCTTCCTCACCAGGAAGGACCCAGACCTGGAGTGTGACGAGGAGCTGGTC
CGGGAGGTGCTGTTTGACGCGGTGGTGACAGCCCCCATGGAAGCCTACTGGACAGCGCTGGCCCTCAACATGTCCGAGGAGTCTGAACACGTGGTGGACA
TGGCCTTCCTGGGCACCCGGGCTGGCCTCCTGAGAAGCAGCTTGTTCGTGGGCTCCGAGAAGGTCTCCGACAGGAAGTTCCTGACACCTGAGGACGAGGC
CAGCGTGTTCACCCTGGACCGCTTCCCGCTGTGGTACCGCCAGGCCTCAGAGCATCCTGCTGGCAGCTTCGTCTTCAACCTCCGCTGGGCAGAAGGACCA
GAAAGTGCGGGTGAACCCATGGTGGTGACGGCAAGCACAGCTGTGGCGGTGACCGTGGACAAGAGGACAGCCATTGCTGCAGCCGCGGGCGTCCAAATGA
AGCTGGAAT
TCCTCCAGCGCAAATTCTGGGCGGCAACGCGGCAGGATCTGGACTGCTTCGTCATCGACAACAACGGGTTCATTCTGATCTCCAAGAGGTC
CCGAGAGACGGGAAGATTTCTGGGGGAGGTGGATGGTGCTGTCCTGACCCAGCTGCTCAGCATGGGGGTGTTCAGCCAAGTGACTATGTATGACTATCAG
GCCATGTGCAAACCCTCGAGTCACCACCACAGTGCAGCCCAGCCCCTGGTCAGCCCAATTTCTGCCTTCTTGACGGCGACCAGGTGGCTGCTGCAGGAGC
TGGTGCTGTTCCTGCTGGAGTGGAGTGTCTGGGGCTCCTGGTACGACAGAGGGGCCGAGGCCAAAAGTGTCTTCCATCACTCCCACAAACACAAGAAGCA
GGACCCGCTGCAGCCCTGCGACACGGAGTACCCCGTGTTCGTGTACCAGCCGGCCATCCGGGAGGCCAACGGGATCGTGGAGTGCGGGCCCTGCCAGAAG
GTATTTGTGGTGCAGCAGATTCCCAACAGTAACCTCCTCCTCCTGGTGACAGACCCCACCTGTGACTGCAGCATCTTCCCACCAGTGCTGCAGGAGGCGA
CAGAAGTCAAATATAATGCCTCTGTCAAATGTGACCGGATGCGCTCCCAGAAGCTCCGCCGGCGACCAGACTCCTGCCACGCCTTCCATCCAGAGGAGAA
TGCCCAGGACTGCGGCGGCGCCTCGGACACCTCAGCCTCGCCGCCCCTACTCCTGCTGCCTGTGTGTGCCTGGGGGCTACTGCCCCAACTCCTGCGGTGA
CACCACCCAGCCTGACCTGTGTTTTGGCAAGGTGATCCTTCCAGAGCCATCCCAAAAAGTCAGCACTGACATGGGATGCAGCTAACTGCAGTTGGGTCGC
CCCCAGGCCAACGCTCCTCTCAATCCTGGGCTGGTGGCCCCTGGCTCCGGAGAATGCTGGATGGAACAGGAAACCAATCACCTGGCACCACTTTCAAGAT
GCTTCATGGTGCCCGGTACCATCTGCCCTAGGTCTCAACATGAGCATACTTCTGACCTAACCTTCCTGTCTCCTCTTCGGGAAGCCAGCGTGAGCTCAGC
TTGGACCAAGACAAAATAATTTAGTTCTTCCTGTACTCCAGAGTCCAGACCCAGCCAAGAAAGGGTCAGTTGTTTCTGACCCTTTCTGTCGGAGTGGTCT
CTGGTAGAACCCAAGGACTTCTGGGTACTGAGAAGCAGCAGCAGAATGAGGCCAAATGCAGAGATGAGGCTAAGGCAAGAATATGCCCCAACTAAAGCAT
AGATTCCCCAAAGTGAGGCTCATGGTGGGAGGCCACTCACCTTCCTAGCTGCTGCTCGAAAAGGTTTTGACTGTGTTGGGGTGGGGGTTGGGTAAGGGAA
TGGTCAAGACTGAGAAAGGAATGAAATCCATTCAGGAAATATCGACAGGGCTACACGTGATGTCCCCAAACTGCTGCTATTGAAGAACTTCCCAAAACTT
CTTTACAAAGCCCTAAAGGAAAGTTTGCATCTATGAAAAGCCAATAGGCTGAGACATCCAATTGCTGCATGGAAATTGATGTACATTCAGGGGACGGCAA
AAATAGCTGTAAAATAGTGAAAAAGAGCAGTGGTTGTGCTCTTTTCTGGCCAATGATTTACAAAAGAATCTACTTGACTCTGTCCCTGGAGTGAAATCCT
TAGGGTTGGAACTTGTGGGAACATTCCAACTTGCTAAGCAGGGTCCACTGGGAGGGAAGCTCTATCTGGGAACTCACCCCCAGCGCACACACATCTCCCC
CAGGGTCCCAAGGCCCCGCAGCTTCCTCCCCCGACCAAACCCCAAGACCTGGATCCCAGGAGACAACAGTCTCCACAGTGAGAGCAACATTAAGGGCAAA
GCCATGGAGAAATGTGGGAGAGGCCGGCCTCAAATCTTTCCATTTAACAAACCCCAGTGATGGGTATGGACAGCATGCAGGGCTTTTGGGGCGCTTCCCC
CCGCTCCTCCATCACCCTCAGCCTCCACACTTCAAAGTTCAAGTTCAAAGCTGTTCAAGTTTCCTACCAGCAAATAGCCCTAACTTGCCTCTAGAGTAGG
CCAAATGCCAACTCTGTAAAACACACTTACATTATCGGTTACAGAATGTCACTCTTACCGTCATGTCTTGCAACAACCCTGTGAGGGCAGTATTAATGCC
CCCTTACAGCAGAAGACACTGCAGCTCGAAGACAGCTTAAGTGGCAGAATAATGCTAGAACAGCTAAGGTTTACATGTACCAAATAACATGTTTCAGCTC
ATTCCATCCTCACAACAGCCCCCTGAAAGTGGGTACTATCATTAGTCCCATGTTATAGAAACTGCAGCAGAGTTGAAAATTGCCTCCAAATTACCGGAAG
AGTGTATGAAGATTGAATGTGATGTATTCACGTAACATGCTTGAAACTGCCTGGCATATACTAAACGCTAAATAAATACATGCTAACTGCGATCATTA
  • refseq_CACNA2D4.F1 (forward primer)
  • refseq_CACNA2D4.R1 (reverse primer)
  • Single exon skipping

Frame 0

RGEASGLKGFMLPQPLPQSLSPRPAC*VGRLFC
ELQLWGREKPSPPRTERLETPGSPRHGRQRPGAS
WGLGGRQTELMVCGCSALLPLPNPRPTMPATPN
FLANPSSSSRWIPLQPMPVAWAFVQKTSALLWL
LLLGTSLSPAWGQAKIPLETVKLWADTFGGDLYN
TVTKYSGSLLLQKKYKDVESSLKIEEVDGLELV
RKFSEDMENMLRRKVEAVQNLVEAAEEADLNHE
FNESLVFDYYNSVLINERDEKGNFVELGAEFLLE
SNAHFSNLPVNTSISSVQLPTNVYNKDPDILNG
VYMSEALNAVFVENFQRDPTLTWQYFGSATGFF
RIYPGIKWTPDENGVITFDCRNRGWYIQAATSPK
DIVILVDVSGSMKGLRMTIAKHTITTILDTLGE
NDFINIIAYNDYVHYIEPCFKGILVQADRDNRE
HFKLLVEELMVKGVGVVDQALREAFQILKQFQEA
KQGSLCNQAIMLISDGAVEDYEPVFEKYNWPDC
KVRVFTYLIGREVSFADRMKWIACNNKGYYTQI
STLADTQENVMEYLHVLSRPMVINHDHDIIWTEA
YMDSKLLSSQAQSLTLLTTVAMPVFSKKNETRS
HGILLGVVGSDVALRELMKLAPRYKLGVHGYAF
LNTNNGYILSHPDLRPLYREGKKLKPKPNYNSVD
LSEVEWEDQAESLRTAMINRETGTLSMDVKVPM
DKGKRVLFLTNDYFFTDISDTPFSLGVVLSRGH
GEYILLGNTSVEEGLHDLLHPDLALAGDWIYCIT
DIDPDHRKLSQLEAMIRFLTRKDPDLECDEELV
REVLFDAVVTAPMEAYWTALALNMSEESEHVVD
MAFLGTRAGLLRSSLFVGSEKVSDRKFLTPEDEA
SVFTLDRFPLWYRQASEHPAGSFVFNLRWAEGP
ESAGEPMVVTASTAVAVTVDKRTAIAAAAGVQM
KLE
FLQRKFWAATRQDLDCFVIDNNGFILISKRS
RETGRFLGEVDGAVLTQLLSMGVFSQVTMYDYQ
AMCKPSSHHHSAAQPLVSPISAFLTATRWLLQE
LVLFLLEWSVWGSWYDRGAEAKSVFHHSHKHKKQ
DPLQPCDTEYPVFVYQPAIREANGIVECGPCQK
VFVVQQIPNSNLLLLVTDPTCDCSIFPPVLQEA
TEVKYNASVKCDRMRSQKLRRRPDSCHAFHPEEN
AQDCGGASDTSASPPLLLLPVCAWGLLPQLLR*
HHPA*PVFWQGDPSRAIPKSQH*HGMQLTAVGS
PPGQRSSQSWAGGPWLRRMLDGTGNQSPGTTFKM
LHGARYHLP*VST*AYF*PNLPVSSSGSQRELS
LDQDKII*FFLYSRVQTQPRKGQLFLTLSVGVV
SGRTQGLLGTEKQQQNEAKCRDEAKARICPN*SI
DSPK*GSWWEATHLPSCCSKRF*LCWGGGWVRE
WSRLRKE*NPFRKYRQGYT*CPQTAAIEELPKT
SLQSPKGKFASMKSQ*AETSNCCMEIDVHSGDGK
NSCKIVKKSSGCALFWPMIYKRIYLTLSLE*NP
*GWNLWEHSNLLSRVHWEGSSIWELTPSAHTSP
PGSQGPAASSPDQTPRPGSQETTVSTVRATLRAK
PWRNVGEAGLKSFHLTNPSDGYGQHAGLLGRFP
PLLHHPQPPHFKVQVQSCSSFLPANSPNLPLE*
AKCQLCKTHLHYRLQNVTLTVMSCNNPVRAVLMP
PYSRRHCSSKTA*VAE*C*NS*GLHVPNNMFQL
IPSSQQPPESGYYH*SHVIETAAELKIASKLPE
ECMKIECDVFT*HA*NCLAYTKR*INTC*LRSL

Frame 1

EERPAD*RVSCCHSLSPSPCLRGLLARWGDSSV
SCSCGAERSPPHPGLRGWRPQARQGTEDKGLGR
PGVWEEGRQSSWSVAALPSFPSPTPGPPCLQLPT
SSQTPAPAAAGFPSSQCPWPGPLCRRPRPSCGC
CF*APPCPLRGDRPRFLWKQ*SYGLTPSAGTCI
TL*PNTQALSCCRRSTRMWSPV*RSRRWMAWSW*
GSSQRTWRTCCGGKSRRSRIWWKLPRRPT*TTN
SMNPWCSTITTRS*STRGTRRATSWSWAPSSSW
SPMLTSATCR*TPPSAACSCPPTCTTKTQIF*ME
STCLKP*MLSSWRTSRETQR*PGNILAVQLDSS
GSIQV*NGHLMRMESLLLTAETAAGTFKLLLLP
RT**FWWT*AAV*RG*G*LLPSTPSPPSWTPWGR
MTSLIS*RTMTTSITSSLVLKGSSSRRTETIES
ISNCWWRS*WSKVWGSWTKP*EKPSRS*SSSKR
PSKEASATRPSCSSATAPWRTTSRCLRSITGQTV
RSEFSLTSLGEKCLLLTA*SGLHATTKATTRRS
QRWRTPRRT*WNTCTCSAAPWSSTTTTTSSGQR
PTWTASSSARRLRA*HCSPLWPCQSSARRTKRDP
MAFSWVWWAQMWP*ES**SWRPGTSLECTDTPF
*TPTMATSSPIPTSGPCTERGRN*NPNLTTTVW
ISPKWSGKTRLNL*EQP*SIGKQVLSRWM*RFRW
IKGSEFFS*PMTTSSRTSATPLSVWGWCCPGAT
ENTSFWGTRLWKKACMTCFTQTWPWPVTGSTAS
QILTQTTGSSAS*RP*SASSPGRTQTWSVTRSWS
GRCCLTRW*QPPWKPTGQRWPSTCPRSLNTWWT
WPSWAPGLAS*EAACSWAPRRSPTGSS*HLRTR
PACSPWTASRCGTARPQSILLAASSSTSAGQKDQ
KVRVNPWW*RQAQLWR*PWTRGQPLLQPRASK*
SWN
SSSANSGRQRGRIWTASSSTTTGSF*SPRG
PERREDFWGRWMVLS*PSCSAWGCSAK*LCMTIR
PCANPRVTTTVQPSPWSAQFLPS*RRPGGCCRS
WCCSCWSGVSGAPGTTEGPRPKVSSITPTNTRS
RTRCSPATRSTPCSCTSRPSGRPTGSWSAGPARR
YLWCSRFPTVTSSSW*QTPPVTAASSHQCCRRR
QKSNIMPLSNVTGCAPRSSAGDQTPATPSIQRR
MPRTAAAPRTPQPRRPYSCCLCVPGGYCPNSCGD
TTQPDLCFGKVILPEPSQKVSTDMGCS*LQLGR
PQANAPLNPGLVAPGSGECWMEQETNHLAPLSR
CFMVPGTICPRSQHEHTSDLTFLSPLREASVSSA
WTKTK*FSSSCTPESRPSQERVSCF*PFLSEWS
LVEPKDFWVLRSSSRMRPNAEMRLRQEYAPTKA
*IPQSEAHGGRPLTFLAAARKGFDCVGVGVG*GN
GQD*ERNEIHSGNIDRATRDVPKLLLLKNFPKL
LYKALKESLHL*KANRLRHPIAAWKLMYIQGTA
KIAVK**KRAVVVLFSGQ*FTKEST*LCPWSEIL
RVGTCGNIPTC*AGSTGREALSGNSPPAHTHLP
QGPKAPQLPPPTKPQDLDPRRQQSPQ*EQH*GQ
SHGEMWERPASNLSI*QTPVMGMDSMQGFWGASP
RSSITLSLHTSKFKFKAVQVSYQQIALTCL*SR
PNANSVKHTYIIGYRMSLLPSCLATTL*GQY*C
PLTAEDTAARRQLKWQNNARTAKVYMYQITCFSS
FHPHNSPLKVGTIISPML*KLQQS*KLPPNYRK
SV*RLNVMYSRNMLETAWHILNAK*IHANCDH

Frame 2

RRGQRIKGFHVATASPPVPVSEACLLGGATLL*
AAAVGQREALPTQD*EAGDPRLAKARKTKAWGV
LGSGRKADRAHGLWLLCPPSPPQPQAHHACNSQ
LPRKPQLQQPLDSPPANARGLGLCAEDLGPPVAA
ASRHLPVPCVGTGQDSSGNSEAMG*HLRRGPV*
HCDQILRLSLAAEEVQGCGVQSEDRGGGWLGAG
EEVLRGHGEHAAEESRGGPESGGSCRGGRPEPRI
Q*IPGVRLLQLGPDQREGREGQLRGAGRRVPPG
VQCSLQQPAGEHLHQQRAAAHQRVQQRPRYFKW
SLHV*SLECCLRGELPERPNVDLAIFWQCNWILQ
DLSRYKMDT**EWSHYF*LPKPRLVHSSCYFSQ
GHSDFGGRERQYEGAEDDYCQAHHHHHLGHPGG
E*LH*YHSVQ*LRPLHRALF*RDPRPGGPRQSRA
FQTAGGGVDGQRCGGRGPSPERSLPDPEAVPRG
QARKPLQPGHHAHQRRRRGGLRAGV*EV*LARL
*GPSFHLPHWERSVFC*PHEVDCMQQQRLLHADL
NAGGHPGERDGIPARAQPPHGHQPRPRHHLDRG
LHGQQAPQLAGSEPDTAHHCGHASLQQEERNAI
PWHSPGCGGLRCGPERADEAGAPVQAWSARIRLS
EHQQWLHPLPSRPPAPVQRGEETKTQT*LQQCG
SLRSGVGRPG*ISENSHDQ*GNRYSLDGCEGSD
G*REASSFPDQ*LLLHGHQRHPFQFGGGAVPGPR
RIHPSGEHVCGRRPA*LASPRPGPGR*LDLLHH
RY*PRPPEAQPARGHDPLPHQEGPRPGV*RGAG
PGGAV*RGGDSPHGSLLDSAGPQHVRGV*TRGGH
GLPGHPGWPPEKQLVRGLREGLRQEVPDT*GRG
QRVHPGPLPAVVPPGLRASCWQLRLQPPLGRRT
RKCG*THGGDGKHSCGGDRGQEDSHCCSRGRPNE
AGI
PPAQILGGNAAGSGLLRHRQQRVHSDLQEV
PRDGKISGGGGWCCPDPAAQHGGVQPSDYV*LS
GHVQTLESPPQCSPAPGQPNFCLLDGDQVAAAGA
GAVPAGVECLGLLVRQRGRGQKCLPSLPQTQEA
GPAAALRHGVPRVRVPAGHPGGQRDRGVRALPE
GICGAADSQQ*PPPPGDRPHL*LQHLPTSAAGGD
RSQI*CLCQM*PDALPEAPPATRLLPRLPSRGE
CPGLRRRLGHLSLAAPTPAACVCLGATAPTPAV
TPPSLTCVLAR*SFQSHPKKSALTWDAANCSWVA
PRPTLLSILGWWPLAPENAGWNRKPITWHHFQD
ASWCPVPSALGLNMSILLT*PSCLLFGKPA*AQ
LGPRQNNLVLPVLQSPDPAKKGSVVSDPFCRSGL
W*NPRTSGY*EAAAE*GQMQR*G*GKNMPQLKH
RFPKVRLMVGGHSPS*LLLEKVLTVLGWGLGKG
MVKTEKGMKSIQEISTGLHVMSPNCCY*RTSQNF
FTKP*RKVCIYEKPIG*DIQLLHGN*CTFRGRQ
K*L*NSEKEQWLCSFLANDLQKNLLDSVPGVKS
LGLELVGTFQLAKQGPLGGKLYLGTHPQRTHISP
RVPRPRSFLPRPNPKTWIPGDNSLHSESNIKGK
AMEKCGRGRPQIFPFNKPQ*WVWTACRAFGALP
PAPPSPSASTLQSSSSKLFKFPTSK*P*LASRVG
QMPTL*NTLTLSVTECHSYRHVLQQPCEGSINA
PLQQKTLQLEDSLSGRIMLEQLRFTCTK*HVSA
HSILTTAP*KWVLSLVPCYRNCSRVENCLQITGR
VYED*M*CIHVTCLKLPGIY*TLNKYMLTAII

Sequence NM_172364

AGAGGAGAGGCCAGCGGATTAAAGGGTTTCATGTTGCCACAGCCTCTCCCCCAGTCCCTGTCTCCGAGGCCTGCTTGCTAGGTGGGGCGACTCTTCTGTG
AGCTGCAGCTGTGGGGCAGAGAGAAGCCCTCCCCACCCAGGACTGAGAGGCTGGAGACCCCAGGCTCGCCAAGGCACGGAAGACAAAGGCCTGGGGCGTC
CTGGGGTCTGGGAGGAAGGCAGACAGAGCTCATGGTCTGTGGCTGCTCTGCCCTCCTTCCCCTCCCCAACCCCAGGCCCACCATGCCTGCAACTCCCAAC
TTCCTCGCAAACCCCAGCTCCAGCAGCCGCTGGATTCCCCTCCAGCCAATGCCCGTGGCCTGGGCCTTTGTGCAGAAGACCTCGGCCCTCCTGTGGCTGC
TGCTTCTAGGCACCTCCCTGTCCCCTGCGTGGGGACAGGCCAAGATTCCTCTGGAAACAGTGAAGCTATGGGCTGACACCTTCGGCGGGGACCTGTATAA
CACTGTGACCAAATACTCAGGCTCTCTCTTGCTGCAGAAGAAGTACAAGGATGTGGAGTCCAGTCTGAAGATCGAGGAGGTGGATGGCTTGGAGCTGGTG
AGGAAGTTCTCAGAGGACATGGAGAACATGCTGCGGAGGAAAGTCGAGGCGGTCCAGAATCTGGTGGAAGCTGCCGAGGAGGCCGACCTGAACCACGAAT
TCAATGAATCCCTGGTGTTCGACTATTACAACTCGGTCCTGATCAACGAGAGGGACGAGAAGGGCAACTTCGTGGAGCTGGGCGCCGAGTTCCTCCTGGA
GTCCAATGCTCACTTCAGCAACCTGCCGGTGAACACCTCCATCAGCAGCGTGCAGCTGCCCACCAACGTGTACAACAAAGACCCAGATATTTTAAATGGA
GTCTACATGTCTGAAGCCTTGAATGCTGTCTTCGTGGAGAACTTCCAGAGAGACCCAACGTTGACCTGGCAATATTTTGGCAGTGCAACTGGATTCTTCA
GGATCTATCCAGGTATAAAATGGACACCTGATGAGAATGGAGTCATTACTTTTGACTGCCGAAACCGCGGCTGGTACATTCAAGCTGCTACTTCTCCCAA
GGACATAGTGATTTTGGTGGACGTGAGCGGCAGTATGAAGGGGCTGAGGATGACTATTGCCAAGCACACCATCACCACCATCTTGGACACCCTGGGGGAG
AATGACTTCATTAATATCATAGCGTACAATGACTACGTCCATTACATCGAGCCTTGTTTTAAAGGGATCCTCGTCCAGGCGGACCGAGACAATCGAGAGC
ATTTCAAACTGCTGGTGGAGGAGTTGATGGTCAAAGGTGTGGGGGTCGTGGACCAAGCCCTGAGAGAAGCCTTCCAGATCCTGAAGCAGTTCCAAGAGGC
CAAGCAAGGAAGCCTCTGCAACCAGGCCATCATGCTCATCAGCGACGGCGCCGTGGAGGACTACGAGCCGGTGTTTGAGAAGTATAACTGGCCAGACTGT
AAGGTCCGAGTTTTCACTTACCTCATTGGGAGAGAAGTGTCTTTTGCTGACCGCATGAAGTGGATTGCATGCAACAACAAAGGCTACTACACGCAGATCT
CAACGCTGGCGGACACCCAGGAGAACGTGATGGAATACCTGCACGTGCTCAGCCGCCCCATGGTCATCAACCACGACCACGACATCATCTGGACAGAGGC
CTACATGGACAGCAAGCTCCTCAGCTCGCAGGCTCAGAGCCTGACACTGCTCACCACTGTGGCCATGCCAGTCTTCAGCAAGAAGAACGAAACGCGATCC
CATGGCATTCTCCTGGGTGTGGTGGGCTCAGATGTGGCCCTGAGAGAGCTGATGAAGCTGGCGCCCCGGTACAAGCTTGGAGTGCACGGATACGCCTTTC
TGAACACCAACAATGGCTACATCCTCTCCCATCCCGACCTCCGGCCCCTGTACAGAGAGGGGAAGAAACTAAAACCCAAACCTAACTACAACAGTGTGGA
TCTCTCCGAAGTGGAGTGGGAAGACCAGGCTGAATCTCTGAGAACAGCCATGATCAATAGGGAAACAGGTACTCTCTCGATGGATGTGAAGGTTCCGATG
GATAAAGGGAAGCGAGTTCTTTTCCTGACCAATGACTACTTCTTCACGGACATCAGCGACACCCCTTTCAGTTTGGGGGTGGTGCTGTCCCGGGGCCACG
GAGAATACATCCTTCTGGGGAACACGTCTGTGGAAGAAGGCCTGCATGACTTGCTTCACCCAGACCTGGCCCTGGCCGGTGACTGGATCTACTGCATCAC
AGATATTGACCCAGACCACCGGAAGCTCAGCCAGCTAGAGGCCATGATCCGCTTCCTCACCAGGAAGGACCCAGACCTGGAGTGTGACGAGGAGCTGGTC
CGGGAGGTGCTGTTTGACGCGGTGGTGACAGCCCCCATGGAAGCCTACTGGACAGCGCTGGCCCTCAACATGTCCGAGGAGTCTGAACACGTGGTGGACA
TGGCCTTCCTGGGCACCCGGGCTGGCCTCCTGAGAAGCAGCTTGTTCGTGGGCTCCGAGAAGGTCTCCGACAGGAAGTTCCTGACACCTGAGGACGAGGC
CAGCGTGTTCACCCTGGACCGCTTCCCGCTGTGGTACCGCCAGGCCTCAGAGCATCCTGCTGGCAGCTTCGTCTTCAACCTCCGCTGGGCAGAAGGACCA
GAAAGTGCGGGTGAACCCATGGTGGTGACGGCAAGCACAGCTGTGGCGGTGACCGTGGACAAGAGGACAGCCATTGCTGCAGCCGCGGGCGTCCAAATGA
AGCTGGAAT
TCCTCCAGCGCAAATTCTGGGCGGCAACGCGGCAGTGCAGCACTGTGGATGGGCCGTGCACACAGAGCTGCGAGGACAGTGATCTGGACTG
CTTCGTCATCGACAACAACGGGTTCATTCTGATCTCCAAGAGGTCCCGAGAGACGGGAAGATTTCTGGGGGAGGTGGATGGTGCTGTCCTGACCCAGCTG
CTCAGCATGGGGGTGTTCAGCCAAGTGACTATGTATGACTATCAGGCCATGTGCAAACCCTCGAGTCACCACCACAGTGCAGCCCAGCCCCTGGTCAGCC
CAATTTCTGCCTTCTTGACGGCGACCAGGTGGCTGCTGCAGGAGCTGGTGCTGTTCCTGCTGGAGTGGAGTGTCTGGGGCTCCTGGTACGACAGAGGGGC
CGAGGCCAAAAGTGTCTTCCATCACTCCCACAAACACAAGAAGCAGGACCCGCTGCAGCCCTGCGACACGGAGTACCCCGTGTTCGTGTACCAGCCGGCC
ATCCGGGAGGCCAACGGGATCGTGGAGTGCGGGCCCTGCCAGAAGGTATTTGTGGTGCAGCAGATTCCCAACAGTAACCTCCTCCTCCTGGTGACAGACC
CCACCTGTGACTGCAGCATCTTCCCACCAGTGCTGCAGGAGGCGACAGAAGTCAAATATAATGCCTCTGTCAAATGTGACCGGATGCGCTCCCAGAAGCT
CCGCCGGCGACCAGACTCCTGCCACGCCTTCCATCCAGAGGAGAATGCCCAGGACTGCGGCGGCGCCTCGGACACCTCAGCCTCGCCGCCCCTACTCCTG
CTGCCTGTGTGTGCCTGGGGGCTACTGCCCCAACTCCTGCGGTGACACCACCCAGCCTGACCTGTGTTTTGGCAAGGTGATCCTTCCAGAGCCATCCCAA
AAAGTCAGCACTGACATGGGATGCAGCTAACTGCAGTTGGGTCGCCCCCAGGCCAACGCTCCTCTCAATCCTGGGCTGGTGGCCCCTGGCTCCGGAGAAT
GCTGGATGGAACAGGAAACCAATCACCTGGCACCACTTTCAAGATGCTTCATGGTGCCCGGTACCATCTGCCCTAGGTCTCAACATGAGCATACTTCTGA
CCTAACCTTCCTGTCTCCTCTTCGGGAAGCCAGCGTGAGCTCAGCTTGGACCAAGACAAAATAATTTAGTTCTTCCTGTACTCCAGAGTCCAGACCCAGC
CAAGAAAGGGTCAGTTGTTTCTGACCCTTTCTGTCGGAGTGGTCTCTGGTAGAACCCAAGGACTTCTGGGTACTGAGAAGCAGCAGCAGAATGAGGCCAA
ATGCAGAGATGAGGCTAAGGCAAGAATATGCCCCAACTAAAGCATAGATTCCCCAAAGTGAGGCTCATGGTGGGAGGCCACTCACCTTCCTAGCTGCTGC
TCGAAAAGGTTTTGACTGTGTTGGGGTGGGGGTTGGGTAAGGGAATGGTCAAGACTGAGAAAGGAATGAAATCCATTCAGGAAATATCGACAGGGCTACA
CGTGATGTCCCCAAACTGCTGCTATTGAAGAACTTCCCAAAACTTCTTTACAAAGCCCTAAAGGAAAGTTTGCATCTATGAAAAGCCAATAGGCTGAGAC
ATCCAATTGCTGCATGGAAATTGATGTACATTCAGGGGACGGCAAAAATAGCTGTAAAATAGTGAAAAAGAGCAGTGGTTGTGCTCTTTTCTGGCCAATG
ATTTACAAAAGAATCTACTTGACTCTGTCCCTGGAGTGAAATCCTTAGGGTTGGAACTTGTGGGAACATTCCAACTTGCTAAGCAGGGTCCACTGGGAGG
GAAGCTCTATCTGGGAACTCACCCCCAGCGCACACACATCTCCCCCAGGGTCCCAAGGCCCCGCAGCTTCCTCCCCCGACCAAACCCCAAGACCTGGATC
CCAGGAGACAACAGTCTCCACAGTGAGAGCAACATTAAGGGCAAAGCCATGGAGAAATGTGGGAGAGGCCGGCCTCAAATCTTTCCATTTAACAAACCCC
AGTGATGGGTATGGACAGCATGCAGGGCTTTTGGGGCGCTTCCCCCCGCTCCTCCATCACCCTCAGCCTCCACACTTCAAAGTTCAAGTTCAAAGCTGTT
CAAGTTTCCTACCAGCAAATAGCCCTAACTTGCCTCTAGAGTAGGCCAAATGCCAACTCTGTAAAACACACTTACATTATCGGTTACAGAATGTCACTCT
TACCGTCATGTCTTGCAACAACCCTGTGAGGGCAGTATTAATGCCCCCTTACAGCAGAAGACACTGCAGCTCGAAGACAGCTTAAGTGGCAGAATAATGC
TAGAACAGCTAAGGTTTACATGTACCAAATAACATGTTTCAGCTCATTCCATCCTCACAACAGCCCCCTGAAAGTGGGTACTATCATTAGTCCCATGTTA
TAGAAACTGCAGCAGAGTTGAAAATTGCCTCCAAATTACCGGAAGAGTGTATGAAGATTGAATGTGATGTATTCACGTAACATGCTTGAAACTGCCTGGC
ATATACTAAACGCTAAATAAATACATGCTAACTGCGATCATTA
  • refseq_CACNA2D4.F1 (forward primer)
  • refseq_CACNA2D4.R1 (reverse primer)
  • Single exon skipping

Frame 0

RGEASGLKGFMLPQPLPQSLSPRPAC*VGRLFC
ELQLWGREKPSPPRTERLETPGSPRHGRQRPGAS
WGLGGRQTELMVCGCSALLPLPNPRPTMPATPN
FLANPSSSSRWIPLQPMPVAWAFVQKTSALLWL
LLLGTSLSPAWGQAKIPLETVKLWADTFGGDLYN
TVTKYSGSLLLQKKYKDVESSLKIEEVDGLELV
RKFSEDMENMLRRKVEAVQNLVEAAEEADLNHE
FNESLVFDYYNSVLINERDEKGNFVELGAEFLLE
SNAHFSNLPVNTSISSVQLPTNVYNKDPDILNG
VYMSEALNAVFVENFQRDPTLTWQYFGSATGFF
RIYPGIKWTPDENGVITFDCRNRGWYIQAATSPK
DIVILVDVSGSMKGLRMTIAKHTITTILDTLGE
NDFINIIAYNDYVHYIEPCFKGILVQADRDNRE
HFKLLVEELMVKGVGVVDQALREAFQILKQFQEA
KQGSLCNQAIMLISDGAVEDYEPVFEKYNWPDC
KVRVFTYLIGREVSFADRMKWIACNNKGYYTQI
STLADTQENVMEYLHVLSRPMVINHDHDIIWTEA
YMDSKLLSSQAQSLTLLTTVAMPVFSKKNETRS
HGILLGVVGSDVALRELMKLAPRYKLGVHGYAF
LNTNNGYILSHPDLRPLYREGKKLKPKPNYNSVD
LSEVEWEDQAESLRTAMINRETGTLSMDVKVPM
DKGKRVLFLTNDYFFTDISDTPFSLGVVLSRGH
GEYILLGNTSVEEGLHDLLHPDLALAGDWIYCIT
DIDPDHRKLSQLEAMIRFLTRKDPDLECDEELV
REVLFDAVVTAPMEAYWTALALNMSEESEHVVD
MAFLGTRAGLLRSSLFVGSEKVSDRKFLTPEDEA
SVFTLDRFPLWYRQASEHPAGSFVFNLRWAEGP
ESAGEPMVVTASTAVAVTVDKRTAIAAAAGVQM
KLE
FLQRKFWAATRQCSTVDGPCTQSCEDSDLDC
FVIDNNGFILISKRSRETGRFLGEVDGAVLTQL
LSMGVFSQVTMYDYQAMCKPSSHHHSAAQPLVS
PISAFLTATRWLLQELVLFLLEWSVWGSWYDRGA
EAKSVFHHSHKHKKQDPLQPCDTEYPVFVYQPA
IREANGIVECGPCQKVFVVQQIPNSNLLLLVTD
PTCDCSIFPPVLQEATEVKYNASVKCDRMRSQKL
RRRPDSCHAFHPEENAQDCGGASDTSASPPLLL
LPVCAWGLLPQLLR*HHPA*PVFWQGDPSRAIP
KSQH*HGMQLTAVGSPPGQRSSQSWAGGPWLRRM
LDGTGNQSPGTTFKMLHGARYHLP*VST*AYF*
PNLPVSSSGSQRELSLDQDKII*FFLYSRVQTQ
PRKGQLFLTLSVGVVSGRTQGLLGTEKQQQNEAK
CRDEAKARICPN*SIDSPK*GSWWEATHLPSCC
SKRF*LCWGGGWVREWSRLRKE*NPFRKYRQGY
T*CPQTAAIEELPKTSLQSPKGKFASMKSQ*AET
SNCCMEIDVHSGDGKNSCKIVKKSSGCALFWPM
IYKRIYLTLSLE*NP*GWNLWEHSNLLSRVHWE
GSSIWELTPSAHTSPPGSQGPAASSPDQTPRPGS
QETTVSTVRATLRAKPWRNVGEAGLKSFHLTNP
SDGYGQHAGLLGRFPPLLHHPQPPHFKVQVQSC
SSFLPANSPNLPLE*AKCQLCKTHLHYRLQNVTL
TVMSCNNPVRAVLMPPYSRRHCSSKTA*VAE*C
*NS*GLHVPNNMFQLIPSSQQPPESGYYH*SHV
IETAAELKIASKLPEECMKIECDVFT*HA*NCLA
YTKR*INTC*LRSL

Frame 1

EERPAD*RVSCCHSLSPSPCLRGLLARWGDSSV
SCSCGAERSPPHPGLRGWRPQARQGTEDKGLGR
PGVWEEGRQSSWSVAALPSFPSPTPGPPCLQLPT
SSQTPAPAAAGFPSSQCPWPGPLCRRPRPSCGC
CF*APPCPLRGDRPRFLWKQ*SYGLTPSAGTCI
TL*PNTQALSCCRRSTRMWSPV*RSRRWMAWSW*
GSSQRTWRTCCGGKSRRSRIWWKLPRRPT*TTN
SMNPWCSTITTRS*STRGTRRATSWSWAPSSSW
SPMLTSATCR*TPPSAACSCPPTCTTKTQIF*ME
STCLKP*MLSSWRTSRETQR*PGNILAVQLDSS
GSIQV*NGHLMRMESLLLTAETAAGTFKLLLLP
RT**FWWT*AAV*RG*G*LLPSTPSPPSWTPWGR
MTSLIS*RTMTTSITSSLVLKGSSSRRTETIES
ISNCWWRS*WSKVWGSWTKP*EKPSRS*SSSKR
PSKEASATRPSCSSATAPWRTTSRCLRSITGQTV
RSEFSLTSLGEKCLLLTA*SGLHATTKATTRRS
QRWRTPRRT*WNTCTCSAAPWSSTTTTTSSGQR
PTWTASSSARRLRA*HCSPLWPCQSSARRTKRDP
MAFSWVWWAQMWP*ES**SWRPGTSLECTDTPF
*TPTMATSSPIPTSGPCTERGRN*NPNLTTTVW
ISPKWSGKTRLNL*EQP*SIGKQVLSRWM*RFRW
IKGSEFFS*PMTTSSRTSATPLSVWGWCCPGAT
ENTSFWGTRLWKKACMTCFTQTWPWPVTGSTAS
QILTQTTGSSAS*RP*SASSPGRTQTWSVTRSWS
GRCCLTRW*QPPWKPTGQRWPSTCPRSLNTWWT
WPSWAPGLAS*EAACSWAPRRSPTGSS*HLRTR
PACSPWTASRCGTARPQSILLAASSSTSAGQKDQ
KVRVNPWW*RQAQLWR*PWTRGQPLLQPRASK*
SWN
SSSANSGRQRGSAALWMGRAHRAARTVIWT
ASSSTTTGSF*SPRGPERREDFWGRWMVLS*PSC
SAWGCSAK*LCMTIRPCANPRVTTTVQPSPWSA
QFLPS*RRPGGCCRSWCCSCWSGVSGAPGTTEG
PRPKVSSITPTNTRSRTRCSPATRSTPCSCTSRP
SGRPTGSWSAGPARRYLWCSRFPTVTSSSW*QT
PPVTAASSHQCCRRRQKSNIMPLSNVTGCAPRS
SAGDQTPATPSIQRRMPRTAAAPRTPQPRRPYSC
CLCVPGGYCPNSCGDTTQPDLCFGKVILPEPSQ
KVSTDMGCS*LQLGRPQANAPLNPGLVAPGSGE
CWMEQETNHLAPLSRCFMVPGTICPRSQHEHTSD
LTFLSPLREASVSSAWTKTK*FSSSCTPESRPS
QERVSCF*PFLSEWSLVEPKDFWVLRSSSRMRP
NAEMRLRQEYAPTKA*IPQSEAHGGRPLTFLAAA
RKGFDCVGVGVG*GNGQD*ERNEIHSGNIDRAT
RDVPKLLLLKNFPKLLYKALKESLHL*KANRLR
HPIAAWKLMYIQGTAKIAVK**KRAVVVLFSGQ*
FTKEST*LCPWSEILRVGTCGNIPTC*AGSTGR
EALSGNSPPAHTHLPQGPKAPQLPPPTKPQDLD
PRRQQSPQ*EQH*GQSHGEMWERPASNLSI*QTP
VMGMDSMQGFWGASPRSSITLSLHTSKFKFKAV
QVSYQQIALTCL*SRPNANSVKHTYIIGYRMSL
LPSCLATTL*GQY*CPLTAEDTAARRQLKWQNNA
RTAKVYMYQITCFSSFHPHNSPLKVGTIISPML
*KLQQS*KLPPNYRKSV*RLNVMYSRNMLETAW
HILNAK*IHANCDH

Frame 2

RRGQRIKGFHVATASPPVPVSEACLLGGATLL*
AAAVGQREALPTQD*EAGDPRLAKARKTKAWGV
LGSGRKADRAHGLWLLCPPSPPQPQAHHACNSQ
LPRKPQLQQPLDSPPANARGLGLCAEDLGPPVAA
ASRHLPVPCVGTGQDSSGNSEAMG*HLRRGPV*
HCDQILRLSLAAEEVQGCGVQSEDRGGGWLGAG
EEVLRGHGEHAAEESRGGPESGGSCRGGRPEPRI
Q*IPGVRLLQLGPDQREGREGQLRGAGRRVPPG
VQCSLQQPAGEHLHQQRAAAHQRVQQRPRYFKW
SLHV*SLECCLRGELPERPNVDLAIFWQCNWILQ
DLSRYKMDT**EWSHYF*LPKPRLVHSSCYFSQ
GHSDFGGRERQYEGAEDDYCQAHHHHHLGHPGG
E*LH*YHSVQ*LRPLHRALF*RDPRPGGPRQSRA
FQTAGGGVDGQRCGGRGPSPERSLPDPEAVPRG
QARKPLQPGHHAHQRRRRGGLRAGV*EV*LARL
*GPSFHLPHWERSVFC*PHEVDCMQQQRLLHADL
NAGGHPGERDGIPARAQPPHGHQPRPRHHLDRG
LHGQQAPQLAGSEPDTAHHCGHASLQQEERNAI
PWHSPGCGGLRCGPERADEAGAPVQAWSARIRLS
EHQQWLHPLPSRPPAPVQRGEETKTQT*LQQCG
SLRSGVGRPG*ISENSHDQ*GNRYSLDGCEGSD
G*REASSFPDQ*LLLHGHQRHPFQFGGGAVPGPR
RIHPSGEHVCGRRPA*LASPRPGPGR*LDLLHH
RY*PRPPEAQPARGHDPLPHQEGPRPGV*RGAG
PGGAV*RGGDSPHGSLLDSAGPQHVRGV*TRGGH
GLPGHPGWPPEKQLVRGLREGLRQEVPDT*GRG
QRVHPGPLPAVVPPGLRASCWQLRLQPPLGRRT
RKCG*THGGDGKHSCGGDRGQEDSHCCSRGRPNE
AGI
PPAQILGGNAAVQHCGWAVHTELRGQ*SGL
LRHRQQRVHSDLQEVPRDGKISGGGGWCCPDPA
AQHGGVQPSDYV*LSGHVQTLESPPQCSPAPGQP
NFCLLDGDQVAAAGAGAVPAGVECLGLLVRQRG
RGQKCLPSLPQTQEAGPAAALRHGVPRVRVPAG
HPGGQRDRGVRALPEGICGAADSQQ*PPPPGDRP
HL*LQHLPTSAAGGDRSQI*CLCQM*PDALPEA
PPATRLLPRLPSRGECPGLRRRLGHLSLAAPTP
AACVCLGATAPTPAVTPPSLTCVLAR*SFQSHPK
KSALTWDAANCSWVAPRPTLLSILGWWPLAPEN
AGWNRKPITWHHFQDASWCPVPSALGLNMSILL
T*PSCLLFGKPA*AQLGPRQNNLVLPVLQSPDPA
KKGSVVSDPFCRSGLW*NPRTSGY*EAAAE*GQ
MQR*G*GKNMPQLKHRFPKVRLMVGGHSPS*LL
LEKVLTVLGWGLGKGMVKTEKGMKSIQEISTGLH
VMSPNCCY*RTSQNFFTKP*RKVCIYEKPIG*D
IQLLHGN*CTFRGRQK*L*NSEKEQWLCSFLAN
DLQKNLLDSVPGVKSLGLELVGTFQLAKQGPLGG
KLYLGTHPQRTHISPRVPRPRSFLPRPNPKTWI
PGDNSLHSESNIKGKAMEKCGRGRPQIFPFNKP
Q*WVWTACRAFGALPPAPPSPSASTLQSSSSKLF
KFPTSK*P*LASRVGQMPTL*NTLTLSVTECHS
YRHVLQQPCEGSINAPLQQKTLQLEDSLSGRIM
LEQLRFTCTK*HVSAHSILTTAP*KWVLSLVPCY
RNCSRVENCLQITGRVYED*M*CIHVTCLKLPG
IY*TLNKYMLTAII

Sequence NM_001005737

AGAGGAGAGGCCAGCGGATTAAAGGGTTTCATGTTGCCACAGCCTCTCCCCCAGTCCCTGTCTCCGAGGCCTGCTTGCTAGGTGGGGCGACTCTTCTGTG
AGCTGCAGCTGTGGGGCAGAGAGAAGCCCTCCCCACCCAGGACTGAGAGGCTGGAGACCCCAGGCTCGCCAAGGCACGGAAGACAAAGGCCTGGGGCGTC
CTGGGGTCTGGGAGGAAGGCAGACAGAGCTCATGGTCTGTGGCTGCTCTGCCCTCCTTCCCCTCCCCAACCCCAGGCCCACCATGCCTGCAACTCCCAAC
TTCCTCGCAAACCCCAGCTCCAGCAGCCGCTGGATTCCCCTCCAGCCAATGCCCGTGGCCTGGGCCTTTGTGCAGAAGACCTCGGCCCTCCTGTGGCTGC
TGCTTCTAGGCACCTCCCTGTCCCCTGCGTGGGGACAGGCCAAGATTCCTCTGGAAACAGTGAAGCTATGGGCTGACACCTTCGGCGGGGACCTGTATAA
CACTGTGACCAAATACTCAGGCTCTCTCTTGCTGCAGAAGAAGTACAAGGATGTGGAGTCCAGTCTGAAGATCGAGGAGGTGGATGGCTTGGAGCTGGTG
AGGAAGTTCTCAGAGGACATGGAGAACATGCTGCGGAGGAAAGTCGAGGCGGTCCAGAATCTGGTGGAAGCTGCCGAGGAGGCCGACCTGAACCACGAAT
TCAATGAATCCCTGGTGTTCGACTATTACAACTCGGTCCTGATCAACGAGAGGGACGAGAAGGGCAACTTCGTGGAGCTGGGCGCCGAGTTCCTCCTGGA
GTCCAATGCTCACTTCAGCAACCTGCCGGTGAACACCTCCATCAGCAGCGTGCAGCTGCCCACCAACGTGTACAACAAAGACCCAGATATTTTAAATGGA
GTCTACATGTCTGAAGCCTTGAATGCTGTCTTCGTGGAGAACTTCCAGAGAGACCCAACGTTGACCTGGCAATATTTTGGCAGTGCAACTGGATTCTTCA
GGATCTATCCAGGTATAAAATGGACACCTGATGAGAATGGAGTCATTACTTTTGACTGCCGAAACCGCGGCTGGTACATTCAAGCTGCTACTTCTCCCAA
GGACATAGTGATTTTGGTGGACGTGAGCGGCAGTATGAAGGGGCTGAGGATGACTATTGCCAAGCACACCATCACCACCATCTTGGACACCCTGGGGGAG
AATGACTTCATTAATATCATAGCGTACAATGACTACGTCCATTACATCGAGCCTTGTTTTAAAGGGATCCTCGTCCAGGCGGACCGAGACAATCGAGAGC
ATTTCAAACTGCTGGTGGAGGAGTTGATGGTCAAAGGTGTGGGGGTCGTGGACCAAGCCCTGAGAGAAGCCTTCCAGATCCTGAAGCAGTTCCAAGAGGC
CAAGCAAGGAAGCCTCTGCAACCAGGCCATCATGCTCATCAGCGACGGCGCCGTGGAGGACTACGAGCCGGTGTTTGAGAAGTATAACTGGCCAGACTGT
AAGGTCCGAGTTTTCACTTACCTCATTGGGAGAGAAGTGTCTTTTGCTGACCGCATGAAGTGGATTGCATGCAACAACAAAGGCTACTACACGCAGATCT
CAACGCTGGCGGACACCCAGGAGAACGTGATGGAATACCTGCACGTGCTCAGCCGCCCCATGGTCATCAACCACGACCACGACATCATCTGGACAGAGGC
CTACATGGACAGCAAGCTCCTCAGCTCGCAGGCTCAGAGCCTGACACTGCTCACCACTGTGGCCATGCCAGTCTTCAGCAAGAAGAACGAAACGCGATCC
CATGGCATTCTCCTGGGTGTGGTGGGCTCAGATGTGGCCCTGAGAGAGCTGATGAAGCTGGCGCCCCGGTACAAGCTTGGAGTGCACGGATACGCCTTTC
TGAACACCAACAATGGCTACATCCTCTCCCATCCCGACCTCCGGCCCCTGTACAGAGAGGGGAAGAAACTAAAACCCAAACCTAACTACAACAGTGTGGA
TCTCTCCGAAGTGGAGTGGGAAGACCAGGCTGAATCTCTGAGAACAGCCATGATCAATAGGGAAACAGGTACTCTCTCGATGGATGTGAAGGTTCCGATG
GATAAAGGGAAGCGAGTTCTTTTCCTGACCAATGACTACTTCTTCACGGACATCAGCGACACCCCTTTCAGTTTGGGGGTGGTGCTGTCCCGGGGCCACG
GAGAATACATCCTTCTGGGGAACACGTCTGTGGAAGAAGGCCTGCATGACTTGCTTCACCCAGACCTGGCCCTGGCCGGTGACTGGATCTACTGCATCAC
AGATATTGACCCAGACCACCGGAAGCTCAGCCAGCTAGAGGCCATGATCCGCTTCCTCACCAGGAAGGACCCAGACCTGGAGTGTGACGAGGAGCTGGTC
CGGGAGGTGCTGTTTGACGCGGTGGTGACAGCCCCCATGGAAGCCTACTGGACAGCGCTGGCCCTCAACATGTCCGAGGAGTCTGAACACGTGGTGGACA
TGGCCTTCCTGGGCACCCGGGCTGGCCTCCTGAGAAGCAGCTTGTTCGTGGGCTCCGAGAAGGTCTCCGACAGGAAGTTCCTGACACCTGAGGACGAGGC
CAGCGTGTTCACCCTGGACCGCTTCCCGCTGTGGTACCGCCAGGCCTCAGAGCATCCTGCTGGCAGCTTCGTCTTCAACCTCCGCTGGGCAGAAGGACCA
GAAAGTGCGGGTGAACCCATGGTGGTGACGGCAAGCACAGCTGTGGCGGTGACCGTGGACAAGAGGACAGCCATTGCTGCAGCCGCGGGCGTCCAAATGA
AGCTGGAAT
TCCTCCAGCGCAAATTCTGGGCGGCAACGCGGCAGTGCAGCACTGTGGATGGGCCGTGCACACAGAGCTGCGAGGACAGTGATCTGGACTG
CTTCGTCATCGACAACAACGGGTTCATTCTGATCTCCAAGAGGTCCCGAGAGAGTGACTATGTATGACTATCAGGCCATGTGCAAACCCTCGAGTCACCA
CCACAGTGCAGCCCAGCCCCTGGTCAGCCCAATTTCTGCCTTCTTGACGGCGACCAGGTGGCTGCTGCAGGAGCTGGTGCTGTTCCTGCTGGAGTGGAGT
GTCTGGGGCTCCTGGTACGACAGAGGGGCCGAGGCCAAAAGTGTCTTCCATCACTCCCACAAACACAAGAAGCAGGACCCGCTGCAGCCCTGCGACACGG
AGTACCCCGTGTTCGTGTACCAGCCGGCCATCCGGGAGGCCAACGGGATCGTGGAGTGCGGGCCCTGCCAGAAGGTATTTGTGGTGCAGCAGATTCCCAA
CAGTAACCTCCTCCTCCTGGTGACAGACCCCACCTGTGACTGCAGCATCTTCCCACCAGTGCTGCAGGAGGCGACAGAAGTCAAATATAATGCCTCTGTC
AAATGTGACCGGATGCGCTCCCAGAAGCTCCGCCGGCGACCAGACTCCTGCCACGCCTTCCATCCAGAGGAGAATGCCCAGGACTGCGGCGGCGCCTCGG
ACACCTCAGCCTCGCCGCCCCTACTCCTGCTGCCTGTGTGTGCCTGGGGGCTACTGCCCCAACTCCTGCGGTGACACCACCCAGCCTGACCTGTGTTTTG
GCAAGGTGATCCTTCCAGAGCCATCCCAAAAAGTCAGCACTGACATGGGATGCAGCTAACTGCAGTTGGGTCGCCCCCAGGCCAACGCTCCTCTCAATCC
TGGGCTGGTGGCCCCTGGCTCCGGAGAATGCTGGATGGAACAGGAAACCAATCACCTGGCACCACTTTCAAGATGCTTCATGGTGCCCGGTACCATCTGC
CCTAGGTCTCAACATGAGCATACTTCTGACCTAACCTTCCTGTCTCCTCTTCGGGAAGCCAGCGTGAGCTCAGCTTGGACCAAGACAAAATAATTTAGTT
CTTCCTGTACTCCAGAGTCCAGACCCAGCCAAGAAAGGGTCAGTTGTTTCTGACCCTTTCTGTCGGAGTGGTCTCTGGTAGAACCCAAGGACTTCTGGGT
ACTGAGAAGCAGCAGCAGAATGAGGCCAAATGCAGAGATGAGGCTAAGGCAAGAATATGCCCCAACTAAAGCATAGATTCCCCAAAGTGAGGCTCATGGT
GGGAGGCCACTCACCTTCCTAGCTGCTGCTCGAAAAGGTTTTGACTGTGTTGGGGTGGGGGTTGGGTAAGGGAATGGTCAAGACTGAGAAAGGAATGAAA
TCCATTCAGGAAATATCGACAGGGCTACACGTGATGTCCCCAAACTGCTGCTATTGAAGAACTTCCCAAAACTTCTTTACAAAGCCCTAAAGGAAAGTTT
GCATCTATGAAAAGCCAATAGGCTGAGACATCCAATTGCTGCATGGAAATTGATGTACATTCAGGGGACGGCAAAAATAGCTGTAAAATAGTGAAAAAGA
GCAGTGGTTGTGCTCTTTTCTGGCCAATGATTTACAAAAGAATCTACTTGACTCTGTCCCTGGAGTGAAATCCTTAGGGTTGGAACTTGTGGGAACATTC
CAACTTGCTAAGCAGGGTCCACTGGGAGGGAAGCTCTATCTGGGAACTCACCCCCAGCGCACACACATCTCCCCCAGGGTCCCAAGGCCCCGCAGCTTCC
TCCCCCGACCAAACCCCAAGACCTGGATCCCAGGAGACAACAGTCTCCACAGTGAGAGCAACATTAAGGGCAAAGCCATGGAGAAATGTGGGAGAGGCCG
GCCTCAAATCTTTCCATTTAACAAACCCCAGTGATGGGTATGGACAGCATGCAGGGCTTTTGGGGCGCTTCCCCCCGCTCCTCCATCACCCTCAGCCTCC
ACACTTCAAAGTTCAAGTTCAAAGCTGTTCAAGTTTCCTACCAGCAAATAGCCCTAACTTGCCTCTAGAGTAGGCCAAATGCCAACTCTGTAAAACACAC
TTACATTATCGGTTACAGAATGTCACTCTTACCGTCATGTCTTGCAACAACCCTGTGAGGGCAGTATTAATGCCCCCTTACAGCAGAAGACACTGCAGCT
CGAAGACAGCTTAAGTGGCAGAATAATGCTAGAACAGCTAAGGTTTACATGTACCAAATAACATGTTTCAGCTCATTCCATCCTCACAACAGCCCCCTGA
AAGTGGGTACTATCATTAGTCCCATGTTATAGAAACTGCAGCAGAGTTGAAAATTGCCTCCAAATTACCGGAAGAGTGTATGAAGATTGAATGTGATGTA
TTCACGTAACATGCTTGAAACTGCCTGGCATATACTAAACGCTAAATAAATACATGCTAACTGCGATCATTA
  • refseq_CACNA2D4.F1 (forward primer)
  • refseq_CACNA2D4.R1 (reverse primer)
  • Single exon skipping

Frame 0

RGEASGLKGFMLPQPLPQSLSPRPAC*VGRLFC
ELQLWGREKPSPPRTERLETPGSPRHGRQRPGAS
WGLGGRQTELMVCGCSALLPLPNPRPTMPATPN
FLANPSSSSRWIPLQPMPVAWAFVQKTSALLWL
LLLGTSLSPAWGQAKIPLETVKLWADTFGGDLYN
TVTKYSGSLLLQKKYKDVESSLKIEEVDGLELV
RKFSEDMENMLRRKVEAVQNLVEAAEEADLNHE
FNESLVFDYYNSVLINERDEKGNFVELGAEFLLE
SNAHFSNLPVNTSISSVQLPTNVYNKDPDILNG
VYMSEALNAVFVENFQRDPTLTWQYFGSATGFF
RIYPGIKWTPDENGVITFDCRNRGWYIQAATSPK
DIVILVDVSGSMKGLRMTIAKHTITTILDTLGE
NDFINIIAYNDYVHYIEPCFKGILVQADRDNRE
HFKLLVEELMVKGVGVVDQALREAFQILKQFQEA
KQGSLCNQAIMLISDGAVEDYEPVFEKYNWPDC
KVRVFTYLIGREVSFADRMKWIACNNKGYYTQI
STLADTQENVMEYLHVLSRPMVINHDHDIIWTEA
YMDSKLLSSQAQSLTLLTTVAMPVFSKKNETRS
HGILLGVVGSDVALRELMKLAPRYKLGVHGYAF
LNTNNGYILSHPDLRPLYREGKKLKPKPNYNSVD
LSEVEWEDQAESLRTAMINRETGTLSMDVKVPM
DKGKRVLFLTNDYFFTDISDTPFSLGVVLSRGH
GEYILLGNTSVEEGLHDLLHPDLALAGDWIYCIT
DIDPDHRKLSQLEAMIRFLTRKDPDLECDEELV
REVLFDAVVTAPMEAYWTALALNMSEESEHVVD
MAFLGTRAGLLRSSLFVGSEKVSDRKFLTPEDEA
SVFTLDRFPLWYRQASEHPAGSFVFNLRWAEGP
ESAGEPMVVTASTAVAVTVDKRTAIAAAAGVQM
KLE
FLQRKFWAATRQCSTVDGPCTQSCEDSDLDC
FVIDNNGFILISKRSRESDYV*LSGHVQTLESP
PQCSPAPGQPNFCLLDGDQVAAAGAGAVPAGVE
CLGLLVRQRGRGQKCLPSLPQTQEAGPAAALRHG
VPRVRVPAGHPGGQRDRGVRALPEGICGAADSQ
Q*PPPPGDRPHL*LQHLPTSAAGGDRSQI*CLC
QM*PDALPEAPPATRLLPRLPSRGECPGLRRRLG
HLSLAAPTPAACVCLGATAPTPAVTPPSLTCVL
AR*SFQSHPKKSALTWDAANCSWVAPRPTLLSI
LGWWPLAPENAGWNRKPITWHHFQDASWCPVPSA
LGLNMSILLT*PSCLLFGKPA*AQLGPRQNNLV
LPVLQSPDPAKKGSVVSDPFCRSGLW*NPRTSG
Y*EAAAE*GQMQR*G*GKNMPQLKHRFPKVRLMV
GGHSPS*LLLEKVLTVLGWGLGKGMVKTEKGMK
SIQEISTGLHVMSPNCCY*RTSQNFFTKP*RKV
CIYEKPIG*DIQLLHGN*CTFRGRQK*L*NSEKE
QWLCSFLANDLQKNLLDSVPGVKSLGLELVGTF
QLAKQGPLGGKLYLGTHPQRTHISPRVPRPRSF
LPRPNPKTWIPGDNSLHSESNIKGKAMEKCGRGR
PQIFPFNKPQ*WVWTACRAFGALPPAPPSPSAS
TLQSSSSKLFKFPTSK*P*LASRVGQMPTL*NT
LTLSVTECHSYRHVLQQPCEGSINAPLQQKTLQL
EDSLSGRIMLEQLRFTCTK*HVSAHSILTTAP*
KWVLSLVPCYRNCSRVENCLQITGRVYED*M*C
IHVTCLKLPGIY*TLNKYMLTAII

Frame 1

EERPAD*RVSCCHSLSPSPCLRGLLARWGDSSV
SCSCGAERSPPHPGLRGWRPQARQGTEDKGLGR
PGVWEEGRQSSWSVAALPSFPSPTPGPPCLQLPT
SSQTPAPAAAGFPSSQCPWPGPLCRRPRPSCGC
CF*APPCPLRGDRPRFLWKQ*SYGLTPSAGTCI
TL*PNTQALSCCRRSTRMWSPV*RSRRWMAWSW*
GSSQRTWRTCCGGKSRRSRIWWKLPRRPT*TTN
SMNPWCSTITTRS*STRGTRRATSWSWAPSSSW
SPMLTSATCR*TPPSAACSCPPTCTTKTQIF*ME
STCLKP*MLSSWRTSRETQR*PGNILAVQLDSS
GSIQV*NGHLMRMESLLLTAETAAGTFKLLLLP
RT**FWWT*AAV*RG*G*LLPSTPSPPSWTPWGR
MTSLIS*RTMTTSITSSLVLKGSSSRRTETIES
ISNCWWRS*WSKVWGSWTKP*EKPSRS*SSSKR
PSKEASATRPSCSSATAPWRTTSRCLRSITGQTV
RSEFSLTSLGEKCLLLTA*SGLHATTKATTRRS
QRWRTPRRT*WNTCTCSAAPWSSTTTTTSSGQR
PTWTASSSARRLRA*HCSPLWPCQSSARRTKRDP
MAFSWVWWAQMWP*ES**SWRPGTSLECTDTPF
*TPTMATSSPIPTSGPCTERGRN*NPNLTTTVW
ISPKWSGKTRLNL*EQP*SIGKQVLSRWM*RFRW
IKGSEFFS*PMTTSSRTSATPLSVWGWCCPGAT
ENTSFWGTRLWKKACMTCFTQTWPWPVTGSTAS
QILTQTTGSSAS*RP*SASSPGRTQTWSVTRSWS
GRCCLTRW*QPPWKPTGQRWPSTCPRSLNTWWT
WPSWAPGLAS*EAACSWAPRRSPTGSS*HLRTR
PACSPWTASRCGTARPQSILLAASSSTSAGQKDQ
KVRVNPWW*RQAQLWR*PWTRGQPLLQPRASK*
SWN
SSSANSGRQRGSAALWMGRAHRAARTVIWT
ASSSTTTGSF*SPRGPERVTMYDYQAMCKPSSHH
HSAAQPLVSPISAFLTATRWLLQELVLFLLEWS
VWGSWYDRGAEAKSVFHHSHKHKKQDPLQPCDT
EYPVFVYQPAIREANGIVECGPCQKVFVVQQIPN
SNLLLLVTDPTCDCSIFPPVLQEATEVKYNASV
KCDRMRSQKLRRRPDSCHAFHPEENAQDCGGAS
DTSASPPLLLLPVCAWGLLPQLLR*HHPA*PVFW
QGDPSRAIPKSQH*HGMQLTAVGSPPGQRSSQS
WAGGPWLRRMLDGTGNQSPGTTFKMLHGARYHL
P*VST*AYF*PNLPVSSSGSQRELSLDQDKII*F
FLYSRVQTQPRKGQLFLTLSVGVVSGRTQGLLG
TEKQQQNEAKCRDEAKARICPN*SIDSPK*GSW
WEATHLPSCCSKRF*LCWGGGWVREWSRLRKE*N
PFRKYRQGYT*CPQTAAIEELPKTSLQSPKGKF
ASMKSQ*AETSNCCMEIDVHSGDGKNSCKIVKK
SSGCALFWPMIYKRIYLTLSLE*NP*GWNLWEHS
NLLSRVHWEGSSIWELTPSAHTSPPGSQGPAAS
SPDQTPRPGSQETTVSTVRATLRAKPWRNVGEA
GLKSFHLTNPSDGYGQHAGLLGRFPPLLHHPQPP
HFKVQVQSCSSFLPANSPNLPLE*AKCQLCKTH
LHYRLQNVTLTVMSCNNPVRAVLMPPYSRRHCS
SKTA*VAE*C*NS*GLHVPNNMFQLIPSSQQPPE
SGYYH*SHVIETAAELKIASKLPEECMKIECDV
FT*HA*NCLAYTKR*INTC*LRSL

Frame 2

RRGQRIKGFHVATASPPVPVSEACLLGGATLL*
AAAVGQREALPTQD*EAGDPRLAKARKTKAWGV
LGSGRKADRAHGLWLLCPPSPPQPQAHHACNSQ
LPRKPQLQQPLDSPPANARGLGLCAEDLGPPVAA
ASRHLPVPCVGTGQDSSGNSEAMG*HLRRGPV*
HCDQILRLSLAAEEVQGCGVQSEDRGGGWLGAG
EEVLRGHGEHAAEESRGGPESGGSCRGGRPEPRI
Q*IPGVRLLQLGPDQREGREGQLRGAGRRVPPG
VQCSLQQPAGEHLHQQRAAAHQRVQQRPRYFKW
SLHV*SLECCLRGELPERPNVDLAIFWQCNWILQ
DLSRYKMDT**EWSHYF*LPKPRLVHSSCYFSQ
GHSDFGGRERQYEGAEDDYCQAHHHHHLGHPGG
E*LH*YHSVQ*LRPLHRALF*RDPRPGGPRQSRA
FQTAGGGVDGQRCGGRGPSPERSLPDPEAVPRG
QARKPLQPGHHAHQRRRRGGLRAGV*EV*LARL
*GPSFHLPHWERSVFC*PHEVDCMQQQRLLHADL
NAGGHPGERDGIPARAQPPHGHQPRPRHHLDRG
LHGQQAPQLAGSEPDTAHHCGHASLQQEERNAI
PWHSPGCGGLRCGPERADEAGAPVQAWSARIRLS
EHQQWLHPLPSRPPAPVQRGEETKTQT*LQQCG
SLRSGVGRPG*ISENSHDQ*GNRYSLDGCEGSD
G*REASSFPDQ*LLLHGHQRHPFQFGGGAVPGPR
RIHPSGEHVCGRRPA*LASPRPGPGR*LDLLHH
RY*PRPPEAQPARGHDPLPHQEGPRPGV*RGAG
PGGAV*RGGDSPHGSLLDSAGPQHVRGV*TRGGH
GLPGHPGWPPEKQLVRGLREGLRQEVPDT*GRG
QRVHPGPLPAVVPPGLRASCWQLRLQPPLGRRT
RKCG*THGGDGKHSCGGDRGQEDSHCCSRGRPNE
AGI
PPAQILGGNAAVQHCGWAVHTELRGQ*SGL
LRHRQQRVHSDLQEVPRE*LCMTIRPCANPRVT
TTVQPSPWSAQFLPS*RRPGGCCRSWCCSCWSGV
SGAPGTTEGPRPKVSSITPTNTRSRTRCSPATR
STPCSCTSRPSGRPTGSWSAGPARRYLWCSRFP
TVTSSSW*QTPPVTAASSHQCCRRRQKSNIMPLS
NVTGCAPRSSAGDQTPATPSIQRRMPRTAAAPR
TPQPRRPYSCCLCVPGGYCPNSCGDTTQPDLCF
GKVILPEPSQKVSTDMGCS*LQLGRPQANAPLNP
GLVAPGSGECWMEQETNHLAPLSRCFMVPGTIC
PRSQHEHTSDLTFLSPLREASVSSAWTKTK*FS
SSCTPESRPSQERVSCF*PFLSEWSLVEPKDFWV
LRSSSRMRPNAEMRLRQEYAPTKA*IPQSEAHG
GRPLTFLAAARKGFDCVGVGVG*GNGQD*ERNE
IHSGNIDRATRDVPKLLLLKNFPKLLYKALKESL
HL*KANRLRHPIAAWKLMYIQGTAKIAVK**KR
AVVVLFSGQ*FTKEST*LCPWSEILRVGTCGNI
PTC*AGSTGREALSGNSPPAHTHLPQGPKAPQLP
PPTKPQDLDPRRQQSPQ*EQH*GQSHGEMWERP
ASNLSI*QTPVMGMDSMQGFWGASPRSSITLSL
HTSKFKFKAVQVSYQQIALTCL*SRPNANSVKHT
YIIGYRMSLLPSCLATTL*GQY*CPLTAEDTAA
RRQLKWQNNARTAKVYMYQITCFSSFHPHNSPL
KVGTIISPML*KLQQS*KLPPNYRKSV*RLNVMY
SRNMLETAWHILNAK*IHANCDH

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