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Sequences

Sequence NM_001005737

AGAGGAGAGGCCAGCGGATTAAAGGGTTTCATGTTGCCACAGCCTCTCCCCCAGTCCCTGTCTCCGAGGCCTGCTTGCTAGGTGGGGCGACTCTTCTGTG AGCTGCAGCTGTGGGGCAGAGAGAAGCCCTCCCCACCCAGGACTGAGAGGCTGGAGACCCCAGGCTCGCCAAGGCACGGAAGACAAAGGCCTGGGGCGTC CTGGGGTCTGGGAGGAAGGCAGACAGAGCTCATGGTCTGTGGCTGCTCTGCCCTCCTTCCCCTCCCCAACCCCAGGCCCACCATGCCTGCAACTCCCAAC TTCCTCGCAAACCCCAGCTCCAGCAGCCGCTGGATTCCCCTCCAGCCAATGCCCGTGGCCTGGGCCTTTGTGCAGAAGACCTCGGCCCTCCTGTGGCTGC TGCTTCTAGGCACCTCCCTGTCCCCTGCGTGGGGACAGGCCAAGATTCCTCTGGAAACAGTGAAGCTATGGGCTGACACCTTCGGCGGGGACCTGTATAA CACTGTGACCAAATACTCAGGCTCTCTCTTGCTGCAGAAGAAGTACAAGGATGTGGAGTCCAGTCTGAAGATCGAGGAGGTGGATGGCTTGGAGCTGGTG AGGAAGTTCTCAGAGGACATGGAGAACATGCTGCGGAGGAAAGTCGAGGCGGTCCAGAATCTGGTGGAAGCTGCCGAGGAGGCCGACCTGAACCACGAAT TCAATGAATCCCTGGTGTTCGACTATTACAACTCGGTCCTGATCAACGAGAGGGACGAGAAGGGCAACTTCGTGGAGCTGGGCGCCGAGTTCCTCCTGGA GTCCAATGCTCACTTCAGCAACCTGCCGGTGAACACCTCCATCAGCAGCGTGCAGCTGCCCACCAACGTGTACAACAAAGACCCAGATATTTTAAATGGA GTCTACATGTCTGAAGCCTTGAATGCTGTCTTCGTGGAGAACTTCCAGAGAGACCCAACGTTGACCTGGCAATATTTTGGCAGTGCAACTGGATTCTTCA GGATCTATCCAGGTATAAAATGGACACCTGATGAGAATGGAGTCATTACTTTTGACTGCCGAAACCGCGGCTGGTACATTCAAGCTGCTACTTCTCCCAA GGACATAGTGATTTTGGTGGACGTGAGCGGCAGTATGAAGGGGCTGAGGATGACTATTGCCAAGCACACCATCACCACCATCTTGGACACCCTGGGGGAG AATGACTTCATTAATATCATAGCGTACAATGACTACGTCCATTACATCGAGCCTTGTTTTAAAGGGATCCTCGTCCAGGCGGACCGAGACAATCGAGAGC ATTTCAAACTGCTGGTGGAGGAGTTGATGGTCAAAGGTGTGGGGGTCGTGGACCAAGCCCTGAGAGAAGCCTTCCAGATCCTGAAGCAGTTCCAAGAGGC CAAGCAAGGAAGCCTCTGCAACCAGGCCATCATGCTCATCAGCGACGGCGCCGTGGAGGACTACGAGCCGGTGTTTGAGAAGTATAACTGGCCAGACTGT AAGGTCCGAGTTTTCACTTACCTCATTGGGAGAGAAGTGTCTTTTGCTGACCGCATGAAGTGGATTGCATGCAACAACAAAGGCTACTACACGCAGATCT CAACGCTGGCGGACACCCAGGAGAACGTGATGGAATACCTGCACGTGCTCAGCCGCCCCATGGTCATCAACCACGACCACGACATCATCTGGACAGAGGC CTACATGGACAGCAAGCTCCTCAGCTCGCAGGCTCAGAGCCTGACACTGCTCACCACTGTGGCCATGCCAGTCTTCAGCAAGAAGAACGAAACGCGATCC CATGGCATTCTCCTGGGTGTGGTGGGCTCAGATGTGGCCCTGAGAGAGCTGATGAAGCTGGCGCCCCGGTACAAGCTTGGAGTGCACGGATACGCCTTTC TGAACACCAACAATGGCTACATCCTCTCCCATCCCGACCTCCGGCCCCTGTACAGAGAGGGGAAGAAACTAAAACCCAAACCTAACTACAACAGTGTGGA TCTCTCCGAAGTGGAGTGGGAAGACCAGGCTGAATCTCTGAGAACAGCCATGATCAATAGGGAAACAGGTACTCTCTCGATGGATGTGAAGGTTCCGATG GATAAAGGGAAGCGAGTTCTTTTCCTGACCAATGACTACTTCTTCACGGACATCAGCGACACCCCTTTCAGTTTGGGGGTGGTGCTGTCCCGGGGCCACG GAGAATACATCCTTCTGGGGAACACGTCTGTGGAAGAAGGCCTGCATGACTTGCTTCACCCAGACCTGGCCCTGGCCGGTGACTGGATCTACTGCATCAC AGATATTGACCCAGACCACCGGAAGCTCAGCCAGCTAGAGGCCATGATCCGCTTCCTCACCAGGAAGGACCCAGACCTGGAGTGTGACGAGGAGCTGGTC CGGGAGGTGCTGTTTGACGCGGTGGTGACAGCCCCCATGGAAGCCTACTGGACAGCGCTGGCCCTCAACATGTCCGAGGAGTCTGAACACGTGGTGGACA TGGCCTTCCTGGGCACCCGGGCTGGCCTCCTGAGAAGCAGCTTGTTCGTGGGCTCCGAGAAGGTCTCCGACAGGAAGTTCCTGACACCTGAGGACGAGGC CAGCGTGTTCACCCTGGACCGCTTCCCGCTGTGGTACCGCCAGGCCTCAGAGCATCCTGCTGGCAGCTTCGTCTTCAACCTCCGCTGGGCAGAAGGACCA GAAAGTGCGGGTGAACCCATGGTGGTGACGGCAAGCACAGCTGTGGCGGTGACCGTGGACAAGAGGACAGCCATTGCTGCAGCCGCGGGCGTCCAAATGA AGCTGGAATTCCTCCAGCGCAAATTCTGGGCGGCAACGCGGCAGTGCAGCACTGTGGATGGGCCGTGCACACAGAGCTGCGAGGACAGTGATCTGGACTG CTTCGTCATCGACAACAACGGGTTCATTCTGATCTCCAAGAGGTCCCGAGAGAGTGACTATGTATGACTATCAGGCCATGTGCAAACCCTCGAGTCACCA CCACAGTGCAGCCCAGCCCCTGGTCAGCCCAATTTCTGCCTTCTTGACGGCGACCAGGTGGCTGCTGCAGGAGCTGGTGCTGTTCCTGCTGGAGTGGAGT GTCTGGGGCTCCTGGTACGACAGAGGGGCCGAGGCCAAAAGTGTCTTCCATCACTCCCACAAACACAAGAAGCAGGACCCGCTGCAGCCCTGCGACACGG AGTACCCCGTGTTCGTGTACCAGCCGGCCATCCGGGAGGCCAACGGGATCGTGGAGTGCGGGCCCTGCCAGAAGGTATTTGTGGTGCAGCAGATTCCCAA CAGTAACCTCCTCCTCCTGGTGACAGACCCCACCTGTGACTGCAGCATCTTCCCACCAGTGCTGCAGGAGGCGACAGAAGTCAAATATAATGCCTCTGTC AAATGTGACCGGATGCGCTCCCAGAAGCTCCGCCGGCGACCAGACTCCTGCCACGCCTTCCATCCAGAGGAGAATGCCCAGGACTGCGGCGGCGCCTCGG ACACCTCAGCCTCGCCGCCCCTACTCCTGCTGCCTGTGTGTGCCTGGGGGCTACTGCCCCAACTCCTGCGGTGACACCACCCAGCCTGACCTGTGTTTTG GCAAGGTGATCCTTCCAGAGCCATCCCAAAAAGTCAGCACTGACATGGGATGCAGCTAACTGCAGTTGGGTCGCCCCCAGGCCAACGCTCCTCTCAATCC TGGGCTGGTGGCCCCTGGCTCCGGAGAATGCTGGATGGAACAGGAAACCAATCACCTGGCACCACTTTCAAGATGCTTCATGGTGCCCGGTACCATCTGC CCTAGGTCTCAACATGAGCATACTTCTGACCTAACCTTCCTGTCTCCTCTTCGGGAAGCCAGCGTGAGCTCAGCTTGGACCAAGACAAAATAATTTAGTT CTTCCTGTACTCCAGAGTCCAGACCCAGCCAAGAAAGGGTCAGTTGTTTCTGACCCTTTCTGTCGGAGTGGTCTCTGGTAGAACCCAAGGACTTCTGGGT ACTGAGAAGCAGCAGCAGAATGAGGCCAAATGCAGAGATGAGGCTAAGGCAAGAATATGCCCCAACTAAAGCATAGATTCCCCAAAGTGAGGCTCATGGT GGGAGGCCACTCACCTTCCTAGCTGCTGCTCGAAAAGGTTTTGACTGTGTTGGGGTGGGGGTTGGGTAAGGGAATGGTCAAGACTGAGAAAGGAATGAAA TCCATTCAGGAAATATCGACAGGGCTACACGTGATGTCCCCAAACTGCTGCTATTGAAGAACTTCCCAAAACTTCTTTACAAAGCCCTAAAGGAAAGTTT GCATCTATGAAAAGCCAATAGGCTGAGACATCCAATTGCTGCATGGAAATTGATGTACATTCAGGGGACGGCAAAAATAGCTGTAAAATAGTGAAAAAGA GCAGTGGTTGTGCTCTTTTCTGGCCAATGATTTACAAAAGAATCTACTTGACTCTGTCCCTGGAGTGAAATCCTTAGGGTTGGAACTTGTGGGAACATTC CAACTTGCTAAGCAGGGTCCACTGGGAGGGAAGCTCTATCTGGGAACTCACCCCCAGCGCACACACATCTCCCCCAGGGTCCCAAGGCCCCGCAGCTTCC TCCCCCGACCAAACCCCAAGACCTGGATCCCAGGAGACAACAGTCTCCACAGTGAGAGCAACATTAAGGGCAAAGCCATGGAGAAATGTGGGAGAGGCCG GCCTCAAATCTTTCCATTTAACAAACCCCAGTGATGGGTATGGACAGCATGCAGGGCTTTTGGGGCGCTTCCCCCCGCTCCTCCATCACCCTCAGCCTCC ACACTTCAAAGTTCAAGTTCAAAGCTGTTCAAGTTTCCTACCAGCAAATAGCCCTAACTTGCCTCTAGAGTAGGCCAAATGCCAACTCTGTAAAACACAC TTACATTATCGGTTACAGAATGTCACTCTTACCGTCATGTCTTGCAACAACCCTGTGAGGGCAGTATTAATGCCCCCTTACAGCAGAAGACACTGCAGCT CGAAGACAGCTTAAGTGGCAGAATAATGCTAGAACAGCTAAGGTTTACATGTACCAAATAACATGTTTCAGCTCATTCCATCCTCACAACAGCCCCCTGA AAGTGGGTACTATCATTAGTCCCATGTTATAGAAACTGCAGCAGAGTTGAAAATTGCCTCCAAATTACCGGAAGAGTGTATGAAGATTGAATGTGATGTA TTCACGTAACATGCTTGAAACTGCCTGGCATATACTAAACGCTAAATAAATACATGCTAACTGCGATCATTA

refseq_CACNA2D4.F3 (forward primer)
refseq_CACNA2D4.R3 (reverse primer)
Single exon skipping

Frame 0

RGEASGLKGFMLPQPLPQSLSPRPAC*VGRLFC ELQLWGREKPSPPRTERLETPGSPRHGRQRPGAS WGLGGRQTELMVCGCSALLPLPNPRPTMPATPN FLANPSSSSRWIPLQPMPVAWAFVQKTSALLWL LLLGTSLSPAWGQAKIPLETVKLWADTFGGDLYN TVTKYSGSLLLQKKYKDVESSLKIEEVDGLELV RKFSEDMENMLRRKVEAVQNLVEAAEEADLNHE FNESLVFDYYNSVLINERDEKGNFVELGAEFLLE SNAHFSNLPVNTSISSVQLPTNVYNKDPDILNG VYMSEALNAVFVENFQRDPTLTWQYFGSATGFF RIYPGIKWTPDENGVITFDCRNRGWYIQAATSPK DIVILVDVSGSMKGLRMTIAKHTITTILDTLGE NDFINIIAYNDYVHYIEPCFKGILVQADRDNRE HFKLLVEELMVKGVGVVDQALREAFQILKQFQEA KQGSLCNQAIMLISDGAVEDYEPVFEKYNWPDC KVRVFTYLIGREVSFADRMKWIACNNKGYYTQI STLADTQENVMEYLHVLSRPMVINHDHDIIWTEA YMDSKLLSSQAQSLTLLTTVAMPVFSKKNETRS HGILLGVVGSDVALRELMKLAPRYKLGVHGYAF LNTNNGYILSHPDLRPLYREGKKLKPKPNYNSVD LSEVEWEDQAESLRTAMINRETGTLSMDVKVPM DKGKRVLFLTNDYFFTDISDTPFSLGVVLSRGH GEYILLGNTSVEEGLHDLLHPDLALAGDWIYCIT DIDPDHRKLSQLEAMIRFLTRKDPDLECDEELV REVLFDAVVTAPMEAYWTALALNMSEESEHVVD MAFLGTRAGLLRSSLFVGSEKVSDRKFLTPEDEA SVFTLDRFPLWYRQASEHPAGSFVFNLRWAEGP ESAGEPMVVTASTAVAVTVDKRTAIAAAAGVQM KLEFLQRKFWAATRQCSTVDGPCTQSCEDSDLDC FVIDNNGFILISKRSRESDYV*LSGHVQTLESP PQCSPAPGQPNFCLLDGDQVAAAGAGAVPAGVE CLGLLVRQRGRGQKCLPSLPQTQEAGPAAALRHG VPRVRVPAGHPGGQRDRGVRALPEGICGAADSQ Q*PPPPGDRPHL*LQHLPTSAAGGDRSQI*CLC QM*PDALPEAPPATRLLPRLPSRGECPGLRRRLG HLSLAAPTPAACVCLGATAPTPAVTPPSLTCVL AR*SFQSHPKKSALTWDAANCSWVAPRPTLLSI LGWWPLAPENAGWNRKPITWHHFQDASWCPVPSA LGLNMSILLT*PSCLLFGKPA*AQLGPRQNNLV LPVLQSPDPAKKGSVVSDPFCRSGLW*NPRTSG Y*EAAAE*GQMQR*G*GKNMPQLKHRFPKVRLMV GGHSPS*LLLEKVLTVLGWGLGKGMVKTEKGMK SIQEISTGLHVMSPNCCY*RTSQNFFTKP*RKV CIYEKPIG*DIQLLHGN*CTFRGRQK*L*NSEKE QWLCSFLANDLQKNLLDSVPGVKSLGLELVGTF QLAKQGPLGGKLYLGTHPQRTHISPRVPRPRSF LPRPNPKTWIPGDNSLHSESNIKGKAMEKCGRGR PQIFPFNKPQ*WVWTACRAFGALPPAPPSPSAS TLQSSSSKLFKFPTSK*P*LASRVGQMPTL*NT LTLSVTECHSYRHVLQQPCEGSINAPLQQKTLQL EDSLSGRIMLEQLRFTCTK*HVSAHSILTTAP* KWVLSLVPCYRNCSRVENCLQITGRVYED*M*C IHVTCLKLPGIY*TLNKYMLTAII

Frame 1

EERPAD*RVSCCHSLSPSPCLRGLLARWGDSSV SCSCGAERSPPHPGLRGWRPQARQGTEDKGLGR PGVWEEGRQSSWSVAALPSFPSPTPGPPCLQLPT SSQTPAPAAAGFPSSQCPWPGPLCRRPRPSCGC CF*APPCPLRGDRPRFLWKQ*SYGLTPSAGTCI TL*PNTQALSCCRRSTRMWSPV*RSRRWMAWSW* GSSQRTWRTCCGGKSRRSRIWWKLPRRPT*TTN SMNPWCSTITTRS*STRGTRRATSWSWAPSSSW SPMLTSATCR*TPPSAACSCPPTCTTKTQIF*ME STCLKP*MLSSWRTSRETQR*PGNILAVQLDSS GSIQV*NGHLMRMESLLLTAETAAGTFKLLLLP RT**FWWT*AAV*RG*G*LLPSTPSPPSWTPWGR MTSLIS*RTMTTSITSSLVLKGSSSRRTETIES ISNCWWRS*WSKVWGSWTKP*EKPSRS*SSSKR PSKEASATRPSCSSATAPWRTTSRCLRSITGQTV RSEFSLTSLGEKCLLLTA*SGLHATTKATTRRS QRWRTPRRT*WNTCTCSAAPWSSTTTTTSSGQR PTWTASSSARRLRA*HCSPLWPCQSSARRTKRDP MAFSWVWWAQMWP*ES**SWRPGTSLECTDTPF *TPTMATSSPIPTSGPCTERGRN*NPNLTTTVW ISPKWSGKTRLNL*EQP*SIGKQVLSRWM*RFRW IKGSEFFS*PMTTSSRTSATPLSVWGWCCPGAT ENTSFWGTRLWKKACMTCFTQTWPWPVTGSTAS QILTQTTGSSAS*RP*SASSPGRTQTWSVTRSWS GRCCLTRW*QPPWKPTGQRWPSTCPRSLNTWWT WPSWAPGLAS*EAACSWAPRRSPTGSS*HLRTR PACSPWTASRCGTARPQSILLAASSSTSAGQKDQ KVRVNPWW*RQAQLWR*PWTRGQPLLQPRASK* SWNSSSANSGRQRGSAALWMGRAHRAARTVIWT ASSSTTTGSF*SPRGPERVTMYDYQAMCKPSSHH HSAAQPLVSPISAFLTATRWLLQELVLFLLEWS VWGSWYDRGAEAKSVFHHSHKHKKQDPLQPCDT EYPVFVYQPAIREANGIVECGPCQKVFVVQQIPN SNLLLLVTDPTCDCSIFPPVLQEATEVKYNASV KCDRMRSQKLRRRPDSCHAFHPEENAQDCGGAS DTSASPPLLLLPVCAWGLLPQLLR*HHPA*PVFW QGDPSRAIPKSQH*HGMQLTAVGSPPGQRSSQS WAGGPWLRRMLDGTGNQSPGTTFKMLHGARYHL P*VST*AYF*PNLPVSSSGSQRELSLDQDKII*F FLYSRVQTQPRKGQLFLTLSVGVVSGRTQGLLG TEKQQQNEAKCRDEAKARICPN*SIDSPK*GSW WEATHLPSCCSKRF*LCWGGGWVREWSRLRKE*N PFRKYRQGYT*CPQTAAIEELPKTSLQSPKGKF ASMKSQ*AETSNCCMEIDVHSGDGKNSCKIVKK SSGCALFWPMIYKRIYLTLSLE*NP*GWNLWEHS NLLSRVHWEGSSIWELTPSAHTSPPGSQGPAAS SPDQTPRPGSQETTVSTVRATLRAKPWRNVGEA GLKSFHLTNPSDGYGQHAGLLGRFPPLLHHPQPP HFKVQVQSCSSFLPANSPNLPLE*AKCQLCKTH LHYRLQNVTLTVMSCNNPVRAVLMPPYSRRHCS SKTA*VAE*C*NS*GLHVPNNMFQLIPSSQQPPE SGYYH*SHVIETAAELKIASKLPEECMKIECDV FT*HA*NCLAYTKR*INTC*LRSL

Frame 2

RRGQRIKGFHVATASPPVPVSEACLLGGATLL* AAAVGQREALPTQD*EAGDPRLAKARKTKAWGV LGSGRKADRAHGLWLLCPPSPPQPQAHHACNSQ LPRKPQLQQPLDSPPANARGLGLCAEDLGPPVAA ASRHLPVPCVGTGQDSSGNSEAMG*HLRRGPV* HCDQILRLSLAAEEVQGCGVQSEDRGGGWLGAG EEVLRGHGEHAAEESRGGPESGGSCRGGRPEPRI Q*IPGVRLLQLGPDQREGREGQLRGAGRRVPPG VQCSLQQPAGEHLHQQRAAAHQRVQQRPRYFKW SLHV*SLECCLRGELPERPNVDLAIFWQCNWILQ DLSRYKMDT**EWSHYF*LPKPRLVHSSCYFSQ GHSDFGGRERQYEGAEDDYCQAHHHHHLGHPGG E*LH*YHSVQ*LRPLHRALF*RDPRPGGPRQSRA FQTAGGGVDGQRCGGRGPSPERSLPDPEAVPRG QARKPLQPGHHAHQRRRRGGLRAGV*EV*LARL *GPSFHLPHWERSVFC*PHEVDCMQQQRLLHADL NAGGHPGERDGIPARAQPPHGHQPRPRHHLDRG LHGQQAPQLAGSEPDTAHHCGHASLQQEERNAI PWHSPGCGGLRCGPERADEAGAPVQAWSARIRLS EHQQWLHPLPSRPPAPVQRGEETKTQT*LQQCG SLRSGVGRPG*ISENSHDQ*GNRYSLDGCEGSD G*REASSFPDQ*LLLHGHQRHPFQFGGGAVPGPR RIHPSGEHVCGRRPA*LASPRPGPGR*LDLLHH RY*PRPPEAQPARGHDPLPHQEGPRPGV*RGAG PGGAV*RGGDSPHGSLLDSAGPQHVRGV*TRGGH GLPGHPGWPPEKQLVRGLREGLRQEVPDT*GRG QRVHPGPLPAVVPPGLRASCWQLRLQPPLGRRT RKCG*THGGDGKHSCGGDRGQEDSHCCSRGRPNE AGIPPAQILGGNAAVQHCGWAVHTELRGQ*SGL LRHRQQRVHSDLQEVPRE*LCMTIRPCANPRVT TTVQPSPWSAQFLPS*RRPGGCCRSWCCSCWSGV SGAPGTTEGPRPKVSSITPTNTRSRTRCSPATR STPCSCTSRPSGRPTGSWSAGPARRYLWCSRFP TVTSSSW*QTPPVTAASSHQCCRRRQKSNIMPLS NVTGCAPRSSAGDQTPATPSIQRRMPRTAAAPR TPQPRRPYSCCLCVPGGYCPNSCGDTTQPDLCF GKVILPEPSQKVSTDMGCS*LQLGRPQANAPLNP GLVAPGSGECWMEQETNHLAPLSRCFMVPGTIC PRSQHEHTSDLTFLSPLREASVSSAWTKTK*FS SSCTPESRPSQERVSCF*PFLSEWSLVEPKDFWV LRSSSRMRPNAEMRLRQEYAPTKA*IPQSEAHG GRPLTFLAAARKGFDCVGVGVG*GNGQD*ERNE IHSGNIDRATRDVPKLLLLKNFPKLLYKALKESL HL*KANRLRHPIAAWKLMYIQGTAKIAVK**KR AVVVLFSGQ*FTKEST*LCPWSEILRVGTCGNI PTC*AGSTGREALSGNSPPAHTHLPQGPKAPQLP PPTKPQDLDPRRQQSPQ*EQH*GQSHGEMWERP ASNLSI*QTPVMGMDSMQGFWGASPRSSITLSL HTSKFKFKAVQVSYQQIALTCL*SRPNANSVKHT YIIGYRMSLLPSCLATTL*GQY*CPLTAEDTAA RRQLKWQNNARTAKVYMYQITCFSSFHPHNSPL KVGTIISPML*KLQQS*KLPPNYRKSV*RLNVMY SRNMLETAWHILNAK*IHANCDH

Sequence NM_001005766

AGAGGAGAGGCCAGCGGATTAAAGGGTTTCATGTTGCCACAGCCTCTCCCCCAGTCCCTGTCTCCGAGGCCTGCTTGCTAGGTGGGGCGACTCTTCTGTG AGCTGCAGCTGTGGGGCAGAGAGAAGCCCTCCCCACCCAGGACTGAGAGGCTGGAGACCCCAGGCTCGCCAAGGCACGGAAGACAAAGGCCTGGGGCGTC CTGGGGTCTGGGAGGAAGGCAGACAGAGCTCATGGTCTGTGGCTGCTCTGCCCTCCTTCCCCTCCCCAACCCCAGGCCCACCATGCCTGCAACTCCCAAC TTCCTCGCAAACCCCAGCTCCAGCAGCCGCTGGATTCCCCTCCAGCCAATGCCCGTGGCCTGGGCCTTTGTGCAGAAGACCTCGGCCCTCCTGTGGCTGC TGCTTCTAGGCACCTCCCTGTCCCCTGCGTGGGGACAGGCCAAGATTCCTCTGGAAACAGTGAAGCTATGGGCTGACACCTTCGGCGGGGACCTGTATAA CACTGTGACCAAATACTCAGGCTCTCTCTTGCTGCAGAAGAAGTACAAGGATGTGGAGTCCAGTCTGAAGATCGAGGAGGTGGATGGCTTGGAGCTGGTG AGGAAGTTCTCAGAGGACATGGAGAACATGCTGCGGAGGAAAGTCGAGGCGGTCCAGAATCTGGTGGAAGCTGCCGAGGAGGCCGACCTGAACCACGAAT TCAATGAATCCCTGGTGTTCGACTATTACAACTCGGTCCTGATCAACGAGAGGGACGAGAAGGGCAACTTCGTGGAGCTGGGCGCCGAGTTCCTCCTGGA GTCCAATGCTCACTTCAGCAACCTGCCGGTGAACACCTCCATCAGCAGCGTGCAGCTGCCCACCAACGTGTACAACAAAGACCCAGATATTTTAAATGGA GTCTACATGTCTGAAGCCTTGAATGCTGTCTTCGTGGAGAACTTCCAGAGAGACCCAACGTTGACCTGGCAATATTTTGGCAGTGCAACTGGATTCTTCA GGATCTATCCAGGTATAAAATGGACACCTGATGAGAATGGAGTCATTACTTTTGACTGCCGAAACCGCGGCTGGTACATTCAAGCTGCTACTTCTCCCAA GGACATAGTGATTTTGGTGGACGTGAGCGGCAGTATGAAGGGGCTGAGGATGACTATTGCCAAGCACACCATCACCACCATCTTGGACACCCTGGGGGAG AATGACTTCATTAATATCATAGCGTACAATGACTACGTCCATTACATCGAGCCTTGTTTTAAAGGGATCCTCGTCCAGGCGGACCGAGACAATCGAGAGC ATTTCAAACTGCTGGTGGAGGAGTTGATGGTCAAAGGTGTGGGGGTCGTGGACCAAGCCCTGAGAGAAGCCTTCCAGATCCTGAAGCAGTTCCAAGAGGC CAAGCAAGGAAGCCTCTGCAACCAGGCCATCATGCTCATCAGCGACGGCGCCGTGGAGGACTACGAGCCGGTGTTTGAGAAGTATAACTGGCCAGACTGT AAGGTCCGAGTTTTCACTTACCTCATTGGGAGAGAAGTGTCTTTTGCTGACCGCATGAAGTGGATTGCATGCAACAACAAAGGCTACTACACGCAGATCT CAACGCTGGCGGACACCCAGGAGAACGTGATGGAATACCTGCACGTGCTCAGCCGCCCCATGGTCATCAACCACGACCACGACATCATCTGGACAGAGGC CTACATGGACAGCAAGCTCCTCAGCTCGCAGGCTCAGAGCCTGACACTGCTCACCACTGTGGCCATGCCAGTCTTCAGCAAGAAGAACGAAACGCGATCC CATGGCATTCTCCTGGGTGTGGTGGGCTCAGATGTGGCCCTGAGAGAGCTGATGAAGCTGGCGCCCCGGTACAAGCTTGGAGTGCACGGATACGCCTTTC TGAACACCAACAATGGCTACATCCTCTCCCATCCCGACCTCCGGCCCCTGTACAGAGAGGGGAAGAAACTAAAACCCAAACCTAACTACAACAGTGTGGA TCTCTCCGAAGTGGAGTGGGAAGACCAGGCTGAATCTCTGAGAACAGCCATGATCAATAGGGAAACAGGTACTCTCTCGATGGATGTGAAGGTTCCGATG GATAAAGGGAAGCGAGTTCTTTTCCTGACCAATGACTACTTCTTCACGGACATCAGCGACACCCCTTTCAGTTTGGGGGTGGTGCTGTCCCGGGGCCACG GAGAATACATCCTTCTGGGGAACACGTCTGTGGAAGAAGGCCTGCATGACTTGCTTCACCCAGACCTGGCCCTGGCCGGTGACTGGATCTACTGCATCAC AGATATTGACCCAGACCACCGGAAGCTCAGCCAGCTAGAGGCCATGATCCGCTTCCTCACCAGGAAGGACCCAGACCTGGAGTGTGACGAGGAGCTGGTC CGGGAGGTGCTGTTTGACGCGGTGGTGACAGCCCCCATGGAAGCCTACTGGACAGCGCTGGCCCTCAACATGTCCGAGGAGTCTGAACACGTGGTGGACA TGGCCTTCCTGGGCACCCGGGCTGGCCTCCTGAGAAGCAGCTTGTTCGTGGGCTCCGAGAAGGTCTCCGACAGGAAGTTCCTGACACCTGAGGACGAGGC CAGCGTGTTCACCCTGGACCGCTTCCCGCTGTGGTACCGCCAGGCCTCAGAGCATCCTGCTGGCAGCTTCGTCTTCAACCTCCGCTGGGCAGAAGGACCA GAAAGTGCGGGTGAACCCATGGTGGTGACGGCAAGCACAGCTGTGGCGGTGACCGTGGACAAGAGGACAGCCATTGCTGCAGCCGCGGGCGTCCAAATGA AGCTGGAATTCCTCCAGCGCAAATTCTGGGCGGCAACGCGGCAGGATCTGGACTGCTTCGTCATCGACAACAACGGGTTCATTCTGATCTCCAAGAGGTC CCGAGAGACGGGAAGATTTCTGGGGGAGGTGGATGGTGCTGTCCTGACCCAGCTGCTCAGCATGGGGGTGTTCAGCCAAGTGACTATGTATGACTATCAG GCCATGTGCAAACCCTCGAGTCACCACCACAGTGCAGCCCAGCCCCTGGTCAGCCCAATTTCTGCCTTCTTGACGGCGACCAGGTGGCTGCTGCAGGAGC TGGTGCTGTTCCTGCTGGAGTGGAGTGTCTGGGGCTCCTGGTACGACAGAGGGGCCGAGGCCAAAAGTGTCTTCCATCACTCCCACAAACACAAGAAGCA GGACCCGCTGCAGCCCTGCGACACGGAGTACCCCGTGTTCGTGTACCAGCCGGCCATCCGGGAGGCCAACGGGATCGTGGAGTGCGGGCCCTGCCAGAAG GTATTTGTGGTGCAGCAGATTCCCAACAGTAACCTCCTCCTCCTGGTGACAGACCCCACCTGTGACTGCAGCATCTTCCCACCAGTGCTGCAGGAGGCGA CAGAAGTCAAATATAATGCCTCTGTCAAATGTGACCGGATGCGCTCCCAGAAGCTCCGCCGGCGACCAGACTCCTGCCACGCCTTCCATCCAGAGGAGAA TGCCCAGGACTGCGGCGGCGCCTCGGACACCTCAGCCTCGCCGCCCCTACTCCTGCTGCCTGTGTGTGCCTGGGGGCTACTGCCCCAACTCCTGCGGTGA CACCACCCAGCCTGACCTGTGTTTTGGCAAGGTGATCCTTCCAGAGCCATCCCAAAAAGTCAGCACTGACATGGGATGCAGCTAACTGCAGTTGGGTCGC CCCCAGGCCAACGCTCCTCTCAATCCTGGGCTGGTGGCCCCTGGCTCCGGAGAATGCTGGATGGAACAGGAAACCAATCACCTGGCACCACTTTCAAGAT GCTTCATGGTGCCCGGTACCATCTGCCCTAGGTCTCAACATGAGCATACTTCTGACCTAACCTTCCTGTCTCCTCTTCGGGAAGCCAGCGTGAGCTCAGC TTGGACCAAGACAAAATAATTTAGTTCTTCCTGTACTCCAGAGTCCAGACCCAGCCAAGAAAGGGTCAGTTGTTTCTGACCCTTTCTGTCGGAGTGGTCT CTGGTAGAACCCAAGGACTTCTGGGTACTGAGAAGCAGCAGCAGAATGAGGCCAAATGCAGAGATGAGGCTAAGGCAAGAATATGCCCCAACTAAAGCAT AGATTCCCCAAAGTGAGGCTCATGGTGGGAGGCCACTCACCTTCCTAGCTGCTGCTCGAAAAGGTTTTGACTGTGTTGGGGTGGGGGTTGGGTAAGGGAA TGGTCAAGACTGAGAAAGGAATGAAATCCATTCAGGAAATATCGACAGGGCTACACGTGATGTCCCCAAACTGCTGCTATTGAAGAACTTCCCAAAACTT CTTTACAAAGCCCTAAAGGAAAGTTTGCATCTATGAAAAGCCAATAGGCTGAGACATCCAATTGCTGCATGGAAATTGATGTACATTCAGGGGACGGCAA AAATAGCTGTAAAATAGTGAAAAAGAGCAGTGGTTGTGCTCTTTTCTGGCCAATGATTTACAAAAGAATCTACTTGACTCTGTCCCTGGAGTGAAATCCT TAGGGTTGGAACTTGTGGGAACATTCCAACTTGCTAAGCAGGGTCCACTGGGAGGGAAGCTCTATCTGGGAACTCACCCCCAGCGCACACACATCTCCCC CAGGGTCCCAAGGCCCCGCAGCTTCCTCCCCCGACCAAACCCCAAGACCTGGATCCCAGGAGACAACAGTCTCCACAGTGAGAGCAACATTAAGGGCAAA GCCATGGAGAAATGTGGGAGAGGCCGGCCTCAAATCTTTCCATTTAACAAACCCCAGTGATGGGTATGGACAGCATGCAGGGCTTTTGGGGCGCTTCCCC CCGCTCCTCCATCACCCTCAGCCTCCACACTTCAAAGTTCAAGTTCAAAGCTGTTCAAGTTTCCTACCAGCAAATAGCCCTAACTTGCCTCTAGAGTAGG CCAAATGCCAACTCTGTAAAACACACTTACATTATCGGTTACAGAATGTCACTCTTACCGTCATGTCTTGCAACAACCCTGTGAGGGCAGTATTAATGCC CCCTTACAGCAGAAGACACTGCAGCTCGAAGACAGCTTAAGTGGCAGAATAATGCTAGAACAGCTAAGGTTTACATGTACCAAATAACATGTTTCAGCTC ATTCCATCCTCACAACAGCCCCCTGAAAGTGGGTACTATCATTAGTCCCATGTTATAGAAACTGCAGCAGAGTTGAAAATTGCCTCCAAATTACCGGAAG AGTGTATGAAGATTGAATGTGATGTATTCACGTAACATGCTTGAAACTGCCTGGCATATACTAAACGCTAAATAAATACATGCTAACTGCGATCATTA

refseq_CACNA2D4.F3 (forward primer)
refseq_CACNA2D4.R3 (reverse primer)
Single exon skipping

Frame 0

RGEASGLKGFMLPQPLPQSLSPRPAC*VGRLFC ELQLWGREKPSPPRTERLETPGSPRHGRQRPGAS WGLGGRQTELMVCGCSALLPLPNPRPTMPATPN FLANPSSSSRWIPLQPMPVAWAFVQKTSALLWL LLLGTSLSPAWGQAKIPLETVKLWADTFGGDLYN TVTKYSGSLLLQKKYKDVESSLKIEEVDGLELV RKFSEDMENMLRRKVEAVQNLVEAAEEADLNHE FNESLVFDYYNSVLINERDEKGNFVELGAEFLLE SNAHFSNLPVNTSISSVQLPTNVYNKDPDILNG VYMSEALNAVFVENFQRDPTLTWQYFGSATGFF RIYPGIKWTPDENGVITFDCRNRGWYIQAATSPK DIVILVDVSGSMKGLRMTIAKHTITTILDTLGE NDFINIIAYNDYVHYIEPCFKGILVQADRDNRE HFKLLVEELMVKGVGVVDQALREAFQILKQFQEA KQGSLCNQAIMLISDGAVEDYEPVFEKYNWPDC KVRVFTYLIGREVSFADRMKWIACNNKGYYTQI STLADTQENVMEYLHVLSRPMVINHDHDIIWTEA YMDSKLLSSQAQSLTLLTTVAMPVFSKKNETRS HGILLGVVGSDVALRELMKLAPRYKLGVHGYAF LNTNNGYILSHPDLRPLYREGKKLKPKPNYNSVD LSEVEWEDQAESLRTAMINRETGTLSMDVKVPM DKGKRVLFLTNDYFFTDISDTPFSLGVVLSRGH GEYILLGNTSVEEGLHDLLHPDLALAGDWIYCIT DIDPDHRKLSQLEAMIRFLTRKDPDLECDEELV REVLFDAVVTAPMEAYWTALALNMSEESEHVVD MAFLGTRAGLLRSSLFVGSEKVSDRKFLTPEDEA SVFTLDRFPLWYRQASEHPAGSFVFNLRWAEGP ESAGEPMVVTASTAVAVTVDKRTAIAAAAGVQM KLEFLQRKFWAATRQDLDCFVIDNNGFILISKRS RETGRFLGEVDGAVLTQLLSMGVFSQVTMYDYQ AMCKPSSHHHSAAQPLVSPISAFLTATRWLLQE LVLFLLEWSVWGSWYDRGAEAKSVFHHSHKHKKQ DPLQPCDTEYPVFVYQPAIREANGIVECGPCQK VFVVQQIPNSNLLLLVTDPTCDCSIFPPVLQEA TEVKYNASVKCDRMRSQKLRRRPDSCHAFHPEEN AQDCGGASDTSASPPLLLLPVCAWGLLPQLLR* HHPA*PVFWQGDPSRAIPKSQH*HGMQLTAVGS PPGQRSSQSWAGGPWLRRMLDGTGNQSPGTTFKM LHGARYHLP*VST*AYF*PNLPVSSSGSQRELS LDQDKII*FFLYSRVQTQPRKGQLFLTLSVGVV SGRTQGLLGTEKQQQNEAKCRDEAKARICPN*SI DSPK*GSWWEATHLPSCCSKRF*LCWGGGWVRE WSRLRKE*NPFRKYRQGYT*CPQTAAIEELPKT SLQSPKGKFASMKSQ*AETSNCCMEIDVHSGDGK NSCKIVKKSSGCALFWPMIYKRIYLTLSLE*NP *GWNLWEHSNLLSRVHWEGSSIWELTPSAHTSP PGSQGPAASSPDQTPRPGSQETTVSTVRATLRAK PWRNVGEAGLKSFHLTNPSDGYGQHAGLLGRFP PLLHHPQPPHFKVQVQSCSSFLPANSPNLPLE* AKCQLCKTHLHYRLQNVTLTVMSCNNPVRAVLMP PYSRRHCSSKTA*VAE*C*NS*GLHVPNNMFQL IPSSQQPPESGYYH*SHVIETAAELKIASKLPE ECMKIECDVFT*HA*NCLAYTKR*INTC*LRSL

Frame 1

EERPAD*RVSCCHSLSPSPCLRGLLARWGDSSV SCSCGAERSPPHPGLRGWRPQARQGTEDKGLGR PGVWEEGRQSSWSVAALPSFPSPTPGPPCLQLPT SSQTPAPAAAGFPSSQCPWPGPLCRRPRPSCGC CF*APPCPLRGDRPRFLWKQ*SYGLTPSAGTCI TL*PNTQALSCCRRSTRMWSPV*RSRRWMAWSW* GSSQRTWRTCCGGKSRRSRIWWKLPRRPT*TTN SMNPWCSTITTRS*STRGTRRATSWSWAPSSSW SPMLTSATCR*TPPSAACSCPPTCTTKTQIF*ME STCLKP*MLSSWRTSRETQR*PGNILAVQLDSS GSIQV*NGHLMRMESLLLTAETAAGTFKLLLLP RT**FWWT*AAV*RG*G*LLPSTPSPPSWTPWGR MTSLIS*RTMTTSITSSLVLKGSSSRRTETIES ISNCWWRS*WSKVWGSWTKP*EKPSRS*SSSKR PSKEASATRPSCSSATAPWRTTSRCLRSITGQTV RSEFSLTSLGEKCLLLTA*SGLHATTKATTRRS QRWRTPRRT*WNTCTCSAAPWSSTTTTTSSGQR PTWTASSSARRLRA*HCSPLWPCQSSARRTKRDP MAFSWVWWAQMWP*ES**SWRPGTSLECTDTPF *TPTMATSSPIPTSGPCTERGRN*NPNLTTTVW ISPKWSGKTRLNL*EQP*SIGKQVLSRWM*RFRW IKGSEFFS*PMTTSSRTSATPLSVWGWCCPGAT ENTSFWGTRLWKKACMTCFTQTWPWPVTGSTAS QILTQTTGSSAS*RP*SASSPGRTQTWSVTRSWS GRCCLTRW*QPPWKPTGQRWPSTCPRSLNTWWT WPSWAPGLAS*EAACSWAPRRSPTGSS*HLRTR PACSPWTASRCGTARPQSILLAASSSTSAGQKDQ KVRVNPWW*RQAQLWR*PWTRGQPLLQPRASK* SWNSSSANSGRQRGRIWTASSSTTTGSF*SPRG PERREDFWGRWMVLS*PSCSAWGCSAK*LCMTIR PCANPRVTTTVQPSPWSAQFLPS*RRPGGCCRS WCCSCWSGVSGAPGTTEGPRPKVSSITPTNTRS RTRCSPATRSTPCSCTSRPSGRPTGSWSAGPARR YLWCSRFPTVTSSSW*QTPPVTAASSHQCCRRR QKSNIMPLSNVTGCAPRSSAGDQTPATPSIQRR MPRTAAAPRTPQPRRPYSCCLCVPGGYCPNSCGD TTQPDLCFGKVILPEPSQKVSTDMGCS*LQLGR PQANAPLNPGLVAPGSGECWMEQETNHLAPLSR CFMVPGTICPRSQHEHTSDLTFLSPLREASVSSA WTKTK*FSSSCTPESRPSQERVSCF*PFLSEWS LVEPKDFWVLRSSSRMRPNAEMRLRQEYAPTKA *IPQSEAHGGRPLTFLAAARKGFDCVGVGVG*GN GQD*ERNEIHSGNIDRATRDVPKLLLLKNFPKL LYKALKESLHL*KANRLRHPIAAWKLMYIQGTA KIAVK**KRAVVVLFSGQ*FTKEST*LCPWSEIL RVGTCGNIPTC*AGSTGREALSGNSPPAHTHLP QGPKAPQLPPPTKPQDLDPRRQQSPQ*EQH*GQ SHGEMWERPASNLSI*QTPVMGMDSMQGFWGASP RSSITLSLHTSKFKFKAVQVSYQQIALTCL*SR PNANSVKHTYIIGYRMSLLPSCLATTL*GQY*C PLTAEDTAARRQLKWQNNARTAKVYMYQITCFSS FHPHNSPLKVGTIISPML*KLQQS*KLPPNYRK SV*RLNVMYSRNMLETAWHILNAK*IHANCDH

Frame 2

RRGQRIKGFHVATASPPVPVSEACLLGGATLL* AAAVGQREALPTQD*EAGDPRLAKARKTKAWGV LGSGRKADRAHGLWLLCPPSPPQPQAHHACNSQ LPRKPQLQQPLDSPPANARGLGLCAEDLGPPVAA ASRHLPVPCVGTGQDSSGNSEAMG*HLRRGPV* HCDQILRLSLAAEEVQGCGVQSEDRGGGWLGAG EEVLRGHGEHAAEESRGGPESGGSCRGGRPEPRI Q*IPGVRLLQLGPDQREGREGQLRGAGRRVPPG VQCSLQQPAGEHLHQQRAAAHQRVQQRPRYFKW SLHV*SLECCLRGELPERPNVDLAIFWQCNWILQ DLSRYKMDT**EWSHYF*LPKPRLVHSSCYFSQ GHSDFGGRERQYEGAEDDYCQAHHHHHLGHPGG E*LH*YHSVQ*LRPLHRALF*RDPRPGGPRQSRA FQTAGGGVDGQRCGGRGPSPERSLPDPEAVPRG QARKPLQPGHHAHQRRRRGGLRAGV*EV*LARL *GPSFHLPHWERSVFC*PHEVDCMQQQRLLHADL NAGGHPGERDGIPARAQPPHGHQPRPRHHLDRG LHGQQAPQLAGSEPDTAHHCGHASLQQEERNAI PWHSPGCGGLRCGPERADEAGAPVQAWSARIRLS EHQQWLHPLPSRPPAPVQRGEETKTQT*LQQCG SLRSGVGRPG*ISENSHDQ*GNRYSLDGCEGSD G*REASSFPDQ*LLLHGHQRHPFQFGGGAVPGPR RIHPSGEHVCGRRPA*LASPRPGPGR*LDLLHH RY*PRPPEAQPARGHDPLPHQEGPRPGV*RGAG PGGAV*RGGDSPHGSLLDSAGPQHVRGV*TRGGH GLPGHPGWPPEKQLVRGLREGLRQEVPDT*GRG QRVHPGPLPAVVPPGLRASCWQLRLQPPLGRRT RKCG*THGGDGKHSCGGDRGQEDSHCCSRGRPNE AGIPPAQILGGNAAGSGLLRHRQQRVHSDLQEV PRDGKISGGGGWCCPDPAAQHGGVQPSDYV*LS GHVQTLESPPQCSPAPGQPNFCLLDGDQVAAAGA GAVPAGVECLGLLVRQRGRGQKCLPSLPQTQEA GPAAALRHGVPRVRVPAGHPGGQRDRGVRALPE GICGAADSQQ*PPPPGDRPHL*LQHLPTSAAGGD RSQI*CLCQM*PDALPEAPPATRLLPRLPSRGE CPGLRRRLGHLSLAAPTPAACVCLGATAPTPAV TPPSLTCVLAR*SFQSHPKKSALTWDAANCSWVA PRPTLLSILGWWPLAPENAGWNRKPITWHHFQD ASWCPVPSALGLNMSILLT*PSCLLFGKPA*AQ LGPRQNNLVLPVLQSPDPAKKGSVVSDPFCRSGL W*NPRTSGY*EAAAE*GQMQR*G*GKNMPQLKH RFPKVRLMVGGHSPS*LLLEKVLTVLGWGLGKG MVKTEKGMKSIQEISTGLHVMSPNCCY*RTSQNF FTKP*RKVCIYEKPIG*DIQLLHGN*CTFRGRQ K*L*NSEKEQWLCSFLANDLQKNLLDSVPGVKS LGLELVGTFQLAKQGPLGGKLYLGTHPQRTHISP RVPRPRSFLPRPNPKTWIPGDNSLHSESNIKGK AMEKCGRGRPQIFPFNKPQ*WVWTACRAFGALP PAPPSPSASTLQSSSSKLFKFPTSK*P*LASRVG QMPTL*NTLTLSVTECHSYRHVLQQPCEGSINA PLQQKTLQLEDSLSGRIMLEQLRFTCTK*HVSA HSILTTAP*KWVLSLVPCYRNCSRVENCLQITGR VYED*M*CIHVTCLKLPGIY*TLNKYMLTAII

Sequence NM_172364

AGAGGAGAGGCCAGCGGATTAAAGGGTTTCATGTTGCCACAGCCTCTCCCCCAGTCCCTGTCTCCGAGGCCTGCTTGCTAGGTGGGGCGACTCTTCTGTG AGCTGCAGCTGTGGGGCAGAGAGAAGCCCTCCCCACCCAGGACTGAGAGGCTGGAGACCCCAGGCTCGCCAAGGCACGGAAGACAAAGGCCTGGGGCGTC CTGGGGTCTGGGAGGAAGGCAGACAGAGCTCATGGTCTGTGGCTGCTCTGCCCTCCTTCCCCTCCCCAACCCCAGGCCCACCATGCCTGCAACTCCCAAC TTCCTCGCAAACCCCAGCTCCAGCAGCCGCTGGATTCCCCTCCAGCCAATGCCCGTGGCCTGGGCCTTTGTGCAGAAGACCTCGGCCCTCCTGTGGCTGC TGCTTCTAGGCACCTCCCTGTCCCCTGCGTGGGGACAGGCCAAGATTCCTCTGGAAACAGTGAAGCTATGGGCTGACACCTTCGGCGGGGACCTGTATAA CACTGTGACCAAATACTCAGGCTCTCTCTTGCTGCAGAAGAAGTACAAGGATGTGGAGTCCAGTCTGAAGATCGAGGAGGTGGATGGCTTGGAGCTGGTG AGGAAGTTCTCAGAGGACATGGAGAACATGCTGCGGAGGAAAGTCGAGGCGGTCCAGAATCTGGTGGAAGCTGCCGAGGAGGCCGACCTGAACCACGAAT TCAATGAATCCCTGGTGTTCGACTATTACAACTCGGTCCTGATCAACGAGAGGGACGAGAAGGGCAACTTCGTGGAGCTGGGCGCCGAGTTCCTCCTGGA GTCCAATGCTCACTTCAGCAACCTGCCGGTGAACACCTCCATCAGCAGCGTGCAGCTGCCCACCAACGTGTACAACAAAGACCCAGATATTTTAAATGGA GTCTACATGTCTGAAGCCTTGAATGCTGTCTTCGTGGAGAACTTCCAGAGAGACCCAACGTTGACCTGGCAATATTTTGGCAGTGCAACTGGATTCTTCA GGATCTATCCAGGTATAAAATGGACACCTGATGAGAATGGAGTCATTACTTTTGACTGCCGAAACCGCGGCTGGTACATTCAAGCTGCTACTTCTCCCAA GGACATAGTGATTTTGGTGGACGTGAGCGGCAGTATGAAGGGGCTGAGGATGACTATTGCCAAGCACACCATCACCACCATCTTGGACACCCTGGGGGAG AATGACTTCATTAATATCATAGCGTACAATGACTACGTCCATTACATCGAGCCTTGTTTTAAAGGGATCCTCGTCCAGGCGGACCGAGACAATCGAGAGC ATTTCAAACTGCTGGTGGAGGAGTTGATGGTCAAAGGTGTGGGGGTCGTGGACCAAGCCCTGAGAGAAGCCTTCCAGATCCTGAAGCAGTTCCAAGAGGC CAAGCAAGGAAGCCTCTGCAACCAGGCCATCATGCTCATCAGCGACGGCGCCGTGGAGGACTACGAGCCGGTGTTTGAGAAGTATAACTGGCCAGACTGT AAGGTCCGAGTTTTCACTTACCTCATTGGGAGAGAAGTGTCTTTTGCTGACCGCATGAAGTGGATTGCATGCAACAACAAAGGCTACTACACGCAGATCT CAACGCTGGCGGACACCCAGGAGAACGTGATGGAATACCTGCACGTGCTCAGCCGCCCCATGGTCATCAACCACGACCACGACATCATCTGGACAGAGGC CTACATGGACAGCAAGCTCCTCAGCTCGCAGGCTCAGAGCCTGACACTGCTCACCACTGTGGCCATGCCAGTCTTCAGCAAGAAGAACGAAACGCGATCC CATGGCATTCTCCTGGGTGTGGTGGGCTCAGATGTGGCCCTGAGAGAGCTGATGAAGCTGGCGCCCCGGTACAAGCTTGGAGTGCACGGATACGCCTTTC TGAACACCAACAATGGCTACATCCTCTCCCATCCCGACCTCCGGCCCCTGTACAGAGAGGGGAAGAAACTAAAACCCAAACCTAACTACAACAGTGTGGA TCTCTCCGAAGTGGAGTGGGAAGACCAGGCTGAATCTCTGAGAACAGCCATGATCAATAGGGAAACAGGTACTCTCTCGATGGATGTGAAGGTTCCGATG GATAAAGGGAAGCGAGTTCTTTTCCTGACCAATGACTACTTCTTCACGGACATCAGCGACACCCCTTTCAGTTTGGGGGTGGTGCTGTCCCGGGGCCACG GAGAATACATCCTTCTGGGGAACACGTCTGTGGAAGAAGGCCTGCATGACTTGCTTCACCCAGACCTGGCCCTGGCCGGTGACTGGATCTACTGCATCAC AGATATTGACCCAGACCACCGGAAGCTCAGCCAGCTAGAGGCCATGATCCGCTTCCTCACCAGGAAGGACCCAGACCTGGAGTGTGACGAGGAGCTGGTC CGGGAGGTGCTGTTTGACGCGGTGGTGACAGCCCCCATGGAAGCCTACTGGACAGCGCTGGCCCTCAACATGTCCGAGGAGTCTGAACACGTGGTGGACA TGGCCTTCCTGGGCACCCGGGCTGGCCTCCTGAGAAGCAGCTTGTTCGTGGGCTCCGAGAAGGTCTCCGACAGGAAGTTCCTGACACCTGAGGACGAGGC CAGCGTGTTCACCCTGGACCGCTTCCCGCTGTGGTACCGCCAGGCCTCAGAGCATCCTGCTGGCAGCTTCGTCTTCAACCTCCGCTGGGCAGAAGGACCA GAAAGTGCGGGTGAACCCATGGTGGTGACGGCAAGCACAGCTGTGGCGGTGACCGTGGACAAGAGGACAGCCATTGCTGCAGCCGCGGGCGTCCAAATGA AGCTGGAATTCCTCCAGCGCAAATTCTGGGCGGCAACGCGGCAGTGCAGCACTGTGGATGGGCCGTGCACACAGAGCTGCGAGGACAGTGATCTGGACTG CTTCGTCATCGACAACAACGGGTTCATTCTGATCTCCAAGAGGTCCCGAGAGACGGGAAGATTTCTGGGGGAGGTGGATGGTGCTGTCCTGACCCAGCTG CTCAGCATGGGGGTGTTCAGCCAAGTGACTATGTATGACTATCAGGCCATGTGCAAACCCTCGAGTCACCACCACAGTGCAGCCCAGCCCCTGGTCAGCC CAATTTCTGCCTTCTTGACGGCGACCAGGTGGCTGCTGCAGGAGCTGGTGCTGTTCCTGCTGGAGTGGAGTGTCTGGGGCTCCTGGTACGACAGAGGGGC CGAGGCCAAAAGTGTCTTCCATCACTCCCACAAACACAAGAAGCAGGACCCGCTGCAGCCCTGCGACACGGAGTACCCCGTGTTCGTGTACCAGCCGGCC ATCCGGGAGGCCAACGGGATCGTGGAGTGCGGGCCCTGCCAGAAGGTATTTGTGGTGCAGCAGATTCCCAACAGTAACCTCCTCCTCCTGGTGACAGACC CCACCTGTGACTGCAGCATCTTCCCACCAGTGCTGCAGGAGGCGACAGAAGTCAAATATAATGCCTCTGTCAAATGTGACCGGATGCGCTCCCAGAAGCT CCGCCGGCGACCAGACTCCTGCCACGCCTTCCATCCAGAGGAGAATGCCCAGGACTGCGGCGGCGCCTCGGACACCTCAGCCTCGCCGCCCCTACTCCTG CTGCCTGTGTGTGCCTGGGGGCTACTGCCCCAACTCCTGCGGTGACACCACCCAGCCTGACCTGTGTTTTGGCAAGGTGATCCTTCCAGAGCCATCCCAA AAAGTCAGCACTGACATGGGATGCAGCTAACTGCAGTTGGGTCGCCCCCAGGCCAACGCTCCTCTCAATCCTGGGCTGGTGGCCCCTGGCTCCGGAGAAT GCTGGATGGAACAGGAAACCAATCACCTGGCACCACTTTCAAGATGCTTCATGGTGCCCGGTACCATCTGCCCTAGGTCTCAACATGAGCATACTTCTGA CCTAACCTTCCTGTCTCCTCTTCGGGAAGCCAGCGTGAGCTCAGCTTGGACCAAGACAAAATAATTTAGTTCTTCCTGTACTCCAGAGTCCAGACCCAGC CAAGAAAGGGTCAGTTGTTTCTGACCCTTTCTGTCGGAGTGGTCTCTGGTAGAACCCAAGGACTTCTGGGTACTGAGAAGCAGCAGCAGAATGAGGCCAA ATGCAGAGATGAGGCTAAGGCAAGAATATGCCCCAACTAAAGCATAGATTCCCCAAAGTGAGGCTCATGGTGGGAGGCCACTCACCTTCCTAGCTGCTGC TCGAAAAGGTTTTGACTGTGTTGGGGTGGGGGTTGGGTAAGGGAATGGTCAAGACTGAGAAAGGAATGAAATCCATTCAGGAAATATCGACAGGGCTACA CGTGATGTCCCCAAACTGCTGCTATTGAAGAACTTCCCAAAACTTCTTTACAAAGCCCTAAAGGAAAGTTTGCATCTATGAAAAGCCAATAGGCTGAGAC ATCCAATTGCTGCATGGAAATTGATGTACATTCAGGGGACGGCAAAAATAGCTGTAAAATAGTGAAAAAGAGCAGTGGTTGTGCTCTTTTCTGGCCAATG ATTTACAAAAGAATCTACTTGACTCTGTCCCTGGAGTGAAATCCTTAGGGTTGGAACTTGTGGGAACATTCCAACTTGCTAAGCAGGGTCCACTGGGAGG GAAGCTCTATCTGGGAACTCACCCCCAGCGCACACACATCTCCCCCAGGGTCCCAAGGCCCCGCAGCTTCCTCCCCCGACCAAACCCCAAGACCTGGATC CCAGGAGACAACAGTCTCCACAGTGAGAGCAACATTAAGGGCAAAGCCATGGAGAAATGTGGGAGAGGCCGGCCTCAAATCTTTCCATTTAACAAACCCC AGTGATGGGTATGGACAGCATGCAGGGCTTTTGGGGCGCTTCCCCCCGCTCCTCCATCACCCTCAGCCTCCACACTTCAAAGTTCAAGTTCAAAGCTGTT CAAGTTTCCTACCAGCAAATAGCCCTAACTTGCCTCTAGAGTAGGCCAAATGCCAACTCTGTAAAACACACTTACATTATCGGTTACAGAATGTCACTCT TACCGTCATGTCTTGCAACAACCCTGTGAGGGCAGTATTAATGCCCCCTTACAGCAGAAGACACTGCAGCTCGAAGACAGCTTAAGTGGCAGAATAATGC TAGAACAGCTAAGGTTTACATGTACCAAATAACATGTTTCAGCTCATTCCATCCTCACAACAGCCCCCTGAAAGTGGGTACTATCATTAGTCCCATGTTA TAGAAACTGCAGCAGAGTTGAAAATTGCCTCCAAATTACCGGAAGAGTGTATGAAGATTGAATGTGATGTATTCACGTAACATGCTTGAAACTGCCTGGC ATATACTAAACGCTAAATAAATACATGCTAACTGCGATCATTA

refseq_CACNA2D4.F3 (forward primer)
refseq_CACNA2D4.R3 (reverse primer)
Single exon skipping

Frame 0

RGEASGLKGFMLPQPLPQSLSPRPAC*VGRLFC ELQLWGREKPSPPRTERLETPGSPRHGRQRPGAS WGLGGRQTELMVCGCSALLPLPNPRPTMPATPN FLANPSSSSRWIPLQPMPVAWAFVQKTSALLWL LLLGTSLSPAWGQAKIPLETVKLWADTFGGDLYN TVTKYSGSLLLQKKYKDVESSLKIEEVDGLELV RKFSEDMENMLRRKVEAVQNLVEAAEEADLNHE FNESLVFDYYNSVLINERDEKGNFVELGAEFLLE SNAHFSNLPVNTSISSVQLPTNVYNKDPDILNG VYMSEALNAVFVENFQRDPTLTWQYFGSATGFF RIYPGIKWTPDENGVITFDCRNRGWYIQAATSPK DIVILVDVSGSMKGLRMTIAKHTITTILDTLGE NDFINIIAYNDYVHYIEPCFKGILVQADRDNRE HFKLLVEELMVKGVGVVDQALREAFQILKQFQEA KQGSLCNQAIMLISDGAVEDYEPVFEKYNWPDC KVRVFTYLIGREVSFADRMKWIACNNKGYYTQI STLADTQENVMEYLHVLSRPMVINHDHDIIWTEA YMDSKLLSSQAQSLTLLTTVAMPVFSKKNETRS HGILLGVVGSDVALRELMKLAPRYKLGVHGYAF LNTNNGYILSHPDLRPLYREGKKLKPKPNYNSVD LSEVEWEDQAESLRTAMINRETGTLSMDVKVPM DKGKRVLFLTNDYFFTDISDTPFSLGVVLSRGH GEYILLGNTSVEEGLHDLLHPDLALAGDWIYCIT DIDPDHRKLSQLEAMIRFLTRKDPDLECDEELV REVLFDAVVTAPMEAYWTALALNMSEESEHVVD MAFLGTRAGLLRSSLFVGSEKVSDRKFLTPEDEA SVFTLDRFPLWYRQASEHPAGSFVFNLRWAEGP ESAGEPMVVTASTAVAVTVDKRTAIAAAAGVQM KLEFLQRKFWAATRQCSTVDGPCTQSCEDSDLDC FVIDNNGFILISKRSRETGRFLGEVDGAVLTQL LSMGVFSQVTMYDYQAMCKPSSHHHSAAQPLVS PISAFLTATRWLLQELVLFLLEWSVWGSWYDRGA EAKSVFHHSHKHKKQDPLQPCDTEYPVFVYQPA IREANGIVECGPCQKVFVVQQIPNSNLLLLVTD PTCDCSIFPPVLQEATEVKYNASVKCDRMRSQKL RRRPDSCHAFHPEENAQDCGGASDTSASPPLLL LPVCAWGLLPQLLR*HHPA*PVFWQGDPSRAIP KSQH*HGMQLTAVGSPPGQRSSQSWAGGPWLRRM LDGTGNQSPGTTFKMLHGARYHLP*VST*AYF* PNLPVSSSGSQRELSLDQDKII*FFLYSRVQTQ PRKGQLFLTLSVGVVSGRTQGLLGTEKQQQNEAK CRDEAKARICPN*SIDSPK*GSWWEATHLPSCC SKRF*LCWGGGWVREWSRLRKE*NPFRKYRQGY T*CPQTAAIEELPKTSLQSPKGKFASMKSQ*AET SNCCMEIDVHSGDGKNSCKIVKKSSGCALFWPM IYKRIYLTLSLE*NP*GWNLWEHSNLLSRVHWE GSSIWELTPSAHTSPPGSQGPAASSPDQTPRPGS QETTVSTVRATLRAKPWRNVGEAGLKSFHLTNP SDGYGQHAGLLGRFPPLLHHPQPPHFKVQVQSC SSFLPANSPNLPLE*AKCQLCKTHLHYRLQNVTL TVMSCNNPVRAVLMPPYSRRHCSSKTA*VAE*C *NS*GLHVPNNMFQLIPSSQQPPESGYYH*SHV IETAAELKIASKLPEECMKIECDVFT*HA*NCLA YTKR*INTC*LRSL

Frame 1

EERPAD*RVSCCHSLSPSPCLRGLLARWGDSSV SCSCGAERSPPHPGLRGWRPQARQGTEDKGLGR PGVWEEGRQSSWSVAALPSFPSPTPGPPCLQLPT SSQTPAPAAAGFPSSQCPWPGPLCRRPRPSCGC CF*APPCPLRGDRPRFLWKQ*SYGLTPSAGTCI TL*PNTQALSCCRRSTRMWSPV*RSRRWMAWSW* GSSQRTWRTCCGGKSRRSRIWWKLPRRPT*TTN SMNPWCSTITTRS*STRGTRRATSWSWAPSSSW SPMLTSATCR*TPPSAACSCPPTCTTKTQIF*ME STCLKP*MLSSWRTSRETQR*PGNILAVQLDSS GSIQV*NGHLMRMESLLLTAETAAGTFKLLLLP RT**FWWT*AAV*RG*G*LLPSTPSPPSWTPWGR MTSLIS*RTMTTSITSSLVLKGSSSRRTETIES ISNCWWRS*WSKVWGSWTKP*EKPSRS*SSSKR PSKEASATRPSCSSATAPWRTTSRCLRSITGQTV RSEFSLTSLGEKCLLLTA*SGLHATTKATTRRS QRWRTPRRT*WNTCTCSAAPWSSTTTTTSSGQR PTWTASSSARRLRA*HCSPLWPCQSSARRTKRDP MAFSWVWWAQMWP*ES**SWRPGTSLECTDTPF *TPTMATSSPIPTSGPCTERGRN*NPNLTTTVW ISPKWSGKTRLNL*EQP*SIGKQVLSRWM*RFRW IKGSEFFS*PMTTSSRTSATPLSVWGWCCPGAT ENTSFWGTRLWKKACMTCFTQTWPWPVTGSTAS QILTQTTGSSAS*RP*SASSPGRTQTWSVTRSWS GRCCLTRW*QPPWKPTGQRWPSTCPRSLNTWWT WPSWAPGLAS*EAACSWAPRRSPTGSS*HLRTR PACSPWTASRCGTARPQSILLAASSSTSAGQKDQ KVRVNPWW*RQAQLWR*PWTRGQPLLQPRASK* SWNSSSANSGRQRGSAALWMGRAHRAARTVIWT ASSSTTTGSF*SPRGPERREDFWGRWMVLS*PSC SAWGCSAK*LCMTIRPCANPRVTTTVQPSPWSA QFLPS*RRPGGCCRSWCCSCWSGVSGAPGTTEG PRPKVSSITPTNTRSRTRCSPATRSTPCSCTSRP SGRPTGSWSAGPARRYLWCSRFPTVTSSSW*QT PPVTAASSHQCCRRRQKSNIMPLSNVTGCAPRS SAGDQTPATPSIQRRMPRTAAAPRTPQPRRPYSC CLCVPGGYCPNSCGDTTQPDLCFGKVILPEPSQ KVSTDMGCS*LQLGRPQANAPLNPGLVAPGSGE CWMEQETNHLAPLSRCFMVPGTICPRSQHEHTSD LTFLSPLREASVSSAWTKTK*FSSSCTPESRPS QERVSCF*PFLSEWSLVEPKDFWVLRSSSRMRP NAEMRLRQEYAPTKA*IPQSEAHGGRPLTFLAAA RKGFDCVGVGVG*GNGQD*ERNEIHSGNIDRAT RDVPKLLLLKNFPKLLYKALKESLHL*KANRLR HPIAAWKLMYIQGTAKIAVK**KRAVVVLFSGQ* FTKEST*LCPWSEILRVGTCGNIPTC*AGSTGR EALSGNSPPAHTHLPQGPKAPQLPPPTKPQDLD PRRQQSPQ*EQH*GQSHGEMWERPASNLSI*QTP VMGMDSMQGFWGASPRSSITLSLHTSKFKFKAV QVSYQQIALTCL*SRPNANSVKHTYIIGYRMSL LPSCLATTL*GQY*CPLTAEDTAARRQLKWQNNA RTAKVYMYQITCFSSFHPHNSPLKVGTIISPML *KLQQS*KLPPNYRKSV*RLNVMYSRNMLETAW HILNAK*IHANCDH

Frame 2

RRGQRIKGFHVATASPPVPVSEACLLGGATLL* AAAVGQREALPTQD*EAGDPRLAKARKTKAWGV LGSGRKADRAHGLWLLCPPSPPQPQAHHACNSQ LPRKPQLQQPLDSPPANARGLGLCAEDLGPPVAA ASRHLPVPCVGTGQDSSGNSEAMG*HLRRGPV* HCDQILRLSLAAEEVQGCGVQSEDRGGGWLGAG EEVLRGHGEHAAEESRGGPESGGSCRGGRPEPRI Q*IPGVRLLQLGPDQREGREGQLRGAGRRVPPG VQCSLQQPAGEHLHQQRAAAHQRVQQRPRYFKW SLHV*SLECCLRGELPERPNVDLAIFWQCNWILQ DLSRYKMDT**EWSHYF*LPKPRLVHSSCYFSQ GHSDFGGRERQYEGAEDDYCQAHHHHHLGHPGG E*LH*YHSVQ*LRPLHRALF*RDPRPGGPRQSRA FQTAGGGVDGQRCGGRGPSPERSLPDPEAVPRG QARKPLQPGHHAHQRRRRGGLRAGV*EV*LARL *GPSFHLPHWERSVFC*PHEVDCMQQQRLLHADL NAGGHPGERDGIPARAQPPHGHQPRPRHHLDRG LHGQQAPQLAGSEPDTAHHCGHASLQQEERNAI PWHSPGCGGLRCGPERADEAGAPVQAWSARIRLS EHQQWLHPLPSRPPAPVQRGEETKTQT*LQQCG SLRSGVGRPG*ISENSHDQ*GNRYSLDGCEGSD G*REASSFPDQ*LLLHGHQRHPFQFGGGAVPGPR RIHPSGEHVCGRRPA*LASPRPGPGR*LDLLHH RY*PRPPEAQPARGHDPLPHQEGPRPGV*RGAG PGGAV*RGGDSPHGSLLDSAGPQHVRGV*TRGGH GLPGHPGWPPEKQLVRGLREGLRQEVPDT*GRG QRVHPGPLPAVVPPGLRASCWQLRLQPPLGRRT RKCG*THGGDGKHSCGGDRGQEDSHCCSRGRPNE AGIPPAQILGGNAAVQHCGWAVHTELRGQ*SGL LRHRQQRVHSDLQEVPRDGKISGGGGWCCPDPA AQHGGVQPSDYV*LSGHVQTLESPPQCSPAPGQP NFCLLDGDQVAAAGAGAVPAGVECLGLLVRQRG RGQKCLPSLPQTQEAGPAAALRHGVPRVRVPAG HPGGQRDRGVRALPEGICGAADSQQ*PPPPGDRP HL*LQHLPTSAAGGDRSQI*CLCQM*PDALPEA PPATRLLPRLPSRGECPGLRRRLGHLSLAAPTP AACVCLGATAPTPAVTPPSLTCVLAR*SFQSHPK KSALTWDAANCSWVAPRPTLLSILGWWPLAPEN AGWNRKPITWHHFQDASWCPVPSALGLNMSILL T*PSCLLFGKPA*AQLGPRQNNLVLPVLQSPDPA KKGSVVSDPFCRSGLW*NPRTSGY*EAAAE*GQ MQR*G*GKNMPQLKHRFPKVRLMVGGHSPS*LL LEKVLTVLGWGLGKGMVKTEKGMKSIQEISTGLH VMSPNCCY*RTSQNFFTKP*RKVCIYEKPIG*D IQLLHGN*CTFRGRQK*L*NSEKEQWLCSFLAN DLQKNLLDSVPGVKSLGLELVGTFQLAKQGPLGG KLYLGTHPQRTHISPRVPRPRSFLPRPNPKTWI PGDNSLHSESNIKGKAMEKCGRGRPQIFPFNKP Q*WVWTACRAFGALPPAPPSPSASTLQSSSSKLF KFPTSK*P*LASRVGQMPTL*NTLTLSVTECHS YRHVLQQPCEGSINAPLQQKTLQLEDSLSGRIM LEQLRFTCTK*HVSAHSILTTAP*KWVLSLVPCY RNCSRVENCLQITGRVYED*M*CIHVTCLKLPG IY*TLNKYMLTAII