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Sequences

Sequence NM_139345

CGCGCCCCTCCCTCCTCGCGGACCTGGCGGTGCCGGCGCCCGGAGTGGCCCTTTAAAAGGCAGCTTATTGTCCGGAGGGGGCGGGCGGGGGGCGCCGACC GCGGCCTGAGGCCCGGCCCCTCCCCTCTCCCTCCCTCTGTCCCCGCGTCGCTCGCTGGCTAGCTCGCTGGCTCGCTCGCCCGTCCGGCGCACGCTCCGCC TCCGTCAGTTGGCTCCGCTGTCGGGTGCGCGGCGTGGAGCGGCAGCCGGTCTGGACGCGCGGCCGGGGCTGGGGGCTGGGAGCGCGGCGCGCAAGATCTC CCCGCGCGAGAGCGGCCCCTGCCACCGGGCGAGGCCTGCGCCGCGATGGCAGAGATGGGCAGTAAAGGGGTGACGGCGGGAAAGATCGCCAGCAACGTGC AGAAGAAGCTCACCCGCGCGCAGGAGAAGGTTCTCCAGAAGCTGGGGAAGGCAGATGAGACCAAGGATGAGCAGTTTGAGCAGTGCGTCCAGAATTTCAA CAAGCAGCTGACGGAGGGCACCCGGCTGCAGAAGGATCTCCGGACCTACCTGGCCTCCGTCAAAGCCATGCACGAGGCTTCCAAGAAGCTGAATGAGTGT CTGCAGGAGGTGTATGAGCCCGATTGGCCCGGCAGGGATGAGGCAAACAAGATCGCAGAGAACAACGACCTGCTGTGGATGGATTACCACCAGAAGCTGG TGGACCAGGCGCTGCTGACCATGGACACGTACCTGGGCCAGTTCCCCGACATCAAGTCACGCATTGCCAAGCGGGGGCGCAAGCTGGTGGACTACGACAG TGCCCGGCACCACTACGAGTCCCTTCAAACTGCCAAAAAGAAGGATGAAGCCAAAATTGCCAAGCCTGTCTCGCTGCTTGAGAAAGCCGCCCCCCAGTGG TGCCAAGGCAAACTGCAGGCTCATCTCGTAGCTCAAACTAACCTGCTCCGAAATCAGGCCGAGGAGGAGCTCATCAAAGCCCAGAAGGTGTTTGAGGAGA TGAATGTGGATCTGCAGGAGGAGCTGCCGTCCCTGTGGAACAGCCGCGTAGGTTTCTACGTCAACACGTTCCAGAGCATCGCGGGCCTGGAGGAAAACTT CCACAAGGAGATGAGCAAGCTCAACCAGAACCTCAATGATGTGCTGGTCGGCCTGGAGAAGCAACACGGGAGCAACACCTTCACGGTCAAGGCCCAGCCC AGTGACAACGCGCCTGCAAAAGGGAACAAGAGCCCTTCGCCTCCAGATGGCTCCCCTGCCGCCACCCCCGAGATCAGAGTCAACCACGAGCCAGAGCCGG CCGGCGGGGCCACGCCCGGGGCCACCCTCCCCAAGTCCCCATCTCAGCCCACAGAGAGTCCAGCCGGCAGCCTGCCTTCCGGGGAGCCCAGCGCTGCCGA GGGCACCTTTGCTGTGTCCTGGCCCAGCCAGACGGCCGAGCCGGGGCCTGCCCAACCAGCAGAGGCCTCGGAGGTGGCGGGTGGGACCCAACCTGCGGCT GGAGCCCAGGAGCCAGGGGAGACGGCGGCAAGTGAAGCAGCCTCCAGCTCTCTTCCTGCTGTCGTGGTGGAGACCTTCCCAGCAACTGTGAATGGCACCG TGGAGGGCGGCAGTGGGGCCGGGCGCTTGGACCTGCCCCCAGGTTTCATGTTCAAGGTACAGGCCCAGCACGACTACACGGCCACTGACACAGACGAGCT GCAGCTCAAGGCTGGTGATGTGGTGCTGGTGATCCCCTTCCAGAACCCTGAAGAGCAGGATGAAGGCTGGCTCATGGGCGTGAAGGAGAGCGACTGGAAC CAGCACAAGGAGCTGGAGAAGTGCCGTGGCGTCTTCCCCGAGAACTTCACTGAGAGGGTCCCATGACGGCGGGGCCCAGGCAGCCTCCGGGCGTGTGAAG AACACCTCCTCCCGAAAAATGTGTGGTTCTTTTTTTTGTTTTGTTTTCGTTTTTCATCTTTTGAAGAGCAAAGGGAAATCAAGAGGAGACCCCCAGGCAG AGGGGCGTTCTCCCAAAGATTAGGTCGTTTTCCAAAGAGCCGCGTCCCGGCAAGTCCGGCGGAATTCACCAGTGTTCCTGAAGCTGCTGTGTCCTCTAGT TGAGTTTCTGGCGCCCCTGCCTGTGCCCGCATGTGTGCCTGGCCGCAGGGCGGGGCTGGGGGCTGCCGAGCCACCATGCTTGCCTGAAGCTTCGGCCGCG CCACCCGGGCAAGGGTCCTCTTTTCCTGGCAGCTGCTGTGGGTGGGGCCCAGACACCAGCCTAGCCTGGCTCTGCCCCGCAGACGGTCTGTGTGCTGTTT GAAAATAAATCTTAGTGTTCAAAACAAAATGAAACAAAAAAAAAATGATAAAAACTCTCAG

refseq_BIN1.F7 (forward primer)
refseq_BIN1.R7 (reverse primer)
Single exon skipping

Frame 0

RAPPSSRTWRCRRPEWPFKRQLIVRRGRAGGAD RGLRPGPSPLPPSVPASLAG*LAGSLARPAHAPP PSVGSAVGCAAWSGSRSGRAAGAGGWERGAQDL PARERPLPPGEACAAMAEMGSKGVTAGKIASNV QKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFN KQLTEGTRLQKDLRTYLASVKAMHEASKKLNEC LQEVYEPDWPGRDEANKIAENNDLLWMDYHQKL VDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDS ARHHYESLQTAKKKDEAKIAKPVSLLEKAAPQW CQGKLQAHLVAQTNLLRNQAEEELIKAQKVFEE MNVDLQEELPSLWNSRVGFYVNTFQSIAGLEENF HKEMSKLNQNLNDVLVGLEKQHGSNTFTVKAQP SDNAPAKGNKSPSPPDGSPAATPEIRVNHEPEP AGGATPGATLPKSPSQPTESPAGSLPSGEPSAAE GTFAVSWPSQTAEPGPAQPAEASEVAGGTQPAA GAQEPGETAASEAASSSLPAVVVETFPATVNGT VEGGSGAGRLDLPPGFMFKVQAQHDYTATDTDEL QLKAGDVVLVIPFQNPEEQDEGWLMGVKESDWN QHKELEKCRGVFPENFTERVP*RRGPGSLRACE EHLLPKNVWFFFLFCFRFSSFEEQREIKRRPPGR GAFSQRLGRFPKSRVPASPAEFTSVPEAAVSSS *VSGAPACARMCAWPQGGAGGCRATMLA*SFGR ATRARVLFSWQLLWVGPRHQPSLALPRRRSVCCL KINLSVQNKMKQKKNDKNSQ

Frame 1

APLPPRGPGGAGARSGPLKGSLLSGGGGRGAPT AA*GPAPPLSLPLSPRRSLASSLARSPVRRTLR LRQLAPLSGARRGAAAGLDARPGLGAGSAARKIS PRESGPCHRARPAPRWQRWAVKG*RRERSPATC RRSSPARRRRFSRSWGRQMRPRMSSLSSASRIS TSS*RRAPGCRRISGPTWPPSKPCTRLPRS*MSV CRRCMSPIGPAGMRQTRSQRTTTCCGWITTRSW WTRRC*PWTRTWASSPTSSHALPSGGASWWTTT VPGTTTSPFKLPKRRMKPKLPSLSRCLRKPPPSG AKANCRLIS*LKLTCSEIRPRRSSSKPRRCLRR *MWICRRSCRPCGTAA*VSTSTRSRASRAWRKT STRR*ASSTRTSMMCWSAWRSNTGATPSRSRPSP VTTRLQKGTRALRLQMAPLPPPPRSESTTSQSR PAGPRPGPPSPSPHLSPQRVQPAACLPGSPALP RAPLLCPGPARRPSRGLPNQQRPRRWRVGPNLRL EPRSQGRRRQVKQPPALFLLSWWRPSQQL*MAP WRAAVGPGAWTCPQVSCSRYRPSTTTRPLTQTS CSSRLVMWCW*SPSRTLKSRMKAGSWA*RRATGT STRSWRSAVASSPRTSLRGSHDGGAQAASGRVK NTSSRKMCGSFFCFVFVFHLLKSKGKSRGDPQA EGRSPKD*VVFQRAASRQVRRNSPVFLKLLCPLV EFLAPLPVPACVPGRRAGLGAAEPPCLPEASAA PPGQGSSFPGSCCGWGPDTSLAWLCPADGLCAV *K*ILVFKTK*NKKKMIKTL

Frame 2

RPSLLADLAVPAPGVAL*KAAYCPEGAGGGRRP RPEARPLPSPSLCPRVARWLARWLARPSGARSA SVSWLRCRVRGVERQPVWTRGRGWGLGARRARS PRARAAPATGRGLRRDGRDGQ*RGDGGKDRQQRA EEAHPRAGEGSPEAGEGR*DQG*AV*AVRPEFQ QAADGGHPAAEGSPDLPGLRQSHARGFQEAE*V SAGGV*ARLARQG*GKQDRREQRPAVDGLPPEAG GPGAADHGHVPGPVPRHQVTHCQAGAQAGGLRQ CPAPLRVPSNCQKEG*SQNCQACLAA*ESRPPV VPRQTAGSSRSSN*PAPKSGRGGAHQSPEGV*GD ECGSAGGAAVPVEQPRRFLRQHVPEHRGPGGKL PQGDEQAQPEPQ*CAGRPGEATREQHLHGQGPA Q*QRACKREQEPFASRWLPCRHPRDQSQPRARAG RRGHARGHPPQVPISAHRESSRQPAFRGAQRCR GHLCCVLAQPDGRAGACPTSRGLGGGGWDPTCG WSPGARGDGGK*SSLQLSSCCRGGDLPSNCEWHR GGRQWGRALGPAPRFHVQGTGPARLHGH*HRRA AAQGW*CGAGDPLPEP*RAG*RLAHGREGERLE PAQGAGEVPWRLPRELH*EGPMTAGPRQPPGV*R TPPPEKCVVLFFVLFSFFIF*RAKGNQEETPRQ RGVLPKIRSFSKEPRPGKSGGIHQCS*SCCVL* LSFWRPCLCPHVCLAAGRGWGLPSHHACLKLRPR HPGKGPLFLAAAVGGAQTPA*PGSAPQTVCVLF ENKS*CSKQNETKKK**KLS

Sequence NM_139350

CGCGCCCCTCCCTCCTCGCGGACCTGGCGGTGCCGGCGCCCGGAGTGGCCCTTTAAAAGGCAGCTTATTGTCCGGAGGGGGCGGGCGGGGGGCGCCGACC GCGGCCTGAGGCCCGGCCCCTCCCCTCTCCCTCCCTCTGTCCCCGCGTCGCTCGCTGGCTAGCTCGCTGGCTCGCTCGCCCGTCCGGCGCACGCTCCGCC TCCGTCAGTTGGCTCCGCTGTCGGGTGCGCGGCGTGGAGCGGCAGCCGGTCTGGACGCGCGGCCGGGGCTGGGGGCTGGGAGCGCGGCGCGCAAGATCTC CCCGCGCGAGAGCGGCCCCTGCCACCGGGCGAGGCCTGCGCCGCGATGGCAGAGATGGGCAGTAAAGGGGTGACGGCGGGAAAGATCGCCAGCAACGTGC AGAAGAAGCTCACCCGCGCGCAGGAGAAGGTTCTCCAGAAGCTGGGGAAGGCAGATGAGACCAAGGATGAGCAGTTTGAGCAGTGCGTCCAGAATTTCAA CAAGCAGCTGACGGAGGGCACCCGGCTGCAGAAGGATCTCCGGACCTACCTGGCCTCCGTCAAAGCCATGCACGAGGCTTCCAAGAAGCTGAATGAGTGT CTGCAGGAGGTGTATGAGCCCGATTGGCCCGGCAGGGATGAGGCAAACAAGATCGCAGAGAACAACGACCTGCTGTGGATGGATTACCACCAGAAGCTGG TGGACCAGGCGCTGCTGACCATGGACACGTACCTGGGCCAGTTCCCCGACATCAAGTCACGCATTGCCAAGCGGGGGCGCAAGCTGGTGGACTACGACAG TGCCCGGCACCACTACGAGTCCCTTCAAACTGCCAAAAAGAAGGATGAAGCCAAAATTGCCAAGGCCGAGGAGGAGCTCATCAAAGCCCAGAAGGTGTTT GAGGAGATGAATGTGGATCTGCAGGAGGAGCTGCCGTCCCTGTGGAACAGCCGCGTAGGTTTCTACGTCAACACGTTCCAGAGCATCGCGGGCCTGGAGG AAAACTTCCACAAGGAGATGAGCAAGCTCAACCAGAACCTCAATGATGTGCTGGTCGGCCTGGAGAAGCAACACGGGAGCAACACCTTCACGGTCAAGGC CCAGCCCAGTGACAACGCGCCTGCAAAAGGGAACAAGAGCCCTTCGCCTCCAGATGGCTCCCCTGCCGCCACCCCCGAGATCAGAGTCAACCACGAGCCA GAGCCGGCCGGCGGGGCCACGCCCGGGGCCACCCTCCCCAAGTCCCCATCTCAGCCAGCAGAGGCCTCGGAGGTGGCGGGTGGGACCCAACCTGCGGCTG GAGCCCAGGAGCCAGGGGAGACGGCGGCAAGTGAAGCAGCCTCCAGCTCTCTTCCTGCTGTCGTGGTGGAGACCTTCCCAGCAACTGTGAATGGCACCGT GGAGGGCGGCAGTGGGGCCGGGCGCTTGGACCTGCCCCCAGGTTTCATGTTCAAGGTACAGGCCCAGCACGACTACACGGCCACTGACACAGACGAGCTG CAGCTCAAGGCTGGTGATGTGGTGCTGGTGATCCCCTTCCAGAACCCTGAAGAGCAGGATGAAGGCTGGCTCATGGGCGTGAAGGAGAGCGACTGGAACC AGCACAAGGAGCTGGAGAAGTGCCGTGGCGTCTTCCCCGAGAACTTCACTGAGAGGGTCCCATGACGGCGGGGCCCAGGCAGCCTCCGGGCGTGTGAAGA ACACCTCCTCCCGAAAAATGTGTGGTTCTTTTTTTTGTTTTGTTTTCGTTTTTCATCTTTTGAAGAGCAAAGGGAAATCAAGAGGAGACCCCCAGGCAGA GGGGCGTTCTCCCAAAGATTAGGTCGTTTTCCAAAGAGCCGCGTCCCGGCAAGTCCGGCGGAATTCACCAGTGTTCCTGAAGCTGCTGTGTCCTCTAGTT GAGTTTCTGGCGCCCCTGCCTGTGCCCGCATGTGTGCCTGGCCGCAGGGCGGGGCTGGGGGCTGCCGAGCCACCATGCTTGCCTGAAGCTTCGGCCGCGC CACCCGGGCAAGGGTCCTCTTTTCCTGGCAGCTGCTGTGGGTGGGGCCCAGACACCAGCCTAGCCTGGCTCTGCCCCGCAGACGGTCTGTGTGCTGTTTG AAAATAAATCTTAGTGTTCAAAACAAAATGAAACAAAAAAAAAATGATAAAAACTCTCAG

refseq_BIN1.F7 (forward primer)
refseq_BIN1.R7 (reverse primer)
Single exon skipping

Frame 0

RAPPSSRTWRCRRPEWPFKRQLIVRRGRAGGAD RGLRPGPSPLPPSVPASLAG*LAGSLARPAHAPP PSVGSAVGCAAWSGSRSGRAAGAGGWERGAQDL PARERPLPPGEACAAMAEMGSKGVTAGKIASNV QKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFN KQLTEGTRLQKDLRTYLASVKAMHEASKKLNEC LQEVYEPDWPGRDEANKIAENNDLLWMDYHQKL VDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDS ARHHYESLQTAKKKDEAKIAKAEEELIKAQKVF EEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLE ENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKA QPSDNAPAKGNKSPSPPDGSPAATPEIRVNHEP EPAGGATPGATLPKSPSQPAEASEVAGGTQPAA GAQEPGETAASEAASSSLPAVVVETFPATVNGTV EGGSGAGRLDLPPGFMFKVQAQHDYTATDTDEL QLKAGDVVLVIPFQNPEEQDEGWLMGVKESDWN QHKELEKCRGVFPENFTERVP*RRGPGSLRACEE HLLPKNVWFFFLFCFRFSSFEEQREIKRRPPGR GAFSQRLGRFPKSRVPASPAEFTSVPEAAVSSS *VSGAPACARMCAWPQGGAGGCRATMLA*SFGRA TRARVLFSWQLLWVGPRHQPSLALPRRRSVCCL KINLSVQNKMKQKKNDKNSQ

Frame 1

APLPPRGPGGAGARSGPLKGSLLSGGGGRGAPT AA*GPAPPLSLPLSPRRSLASSLARSPVRRTLR LRQLAPLSGARRGAAAGLDARPGLGAGSAARKIS PRESGPCHRARPAPRWQRWAVKG*RRERSPATC RRSSPARRRRFSRSWGRQMRPRMSSLSSASRIS TSS*RRAPGCRRISGPTWPPSKPCTRLPRS*MSV CRRCMSPIGPAGMRQTRSQRTTTCCGWITTRSW WTRRC*PWTRTWASSPTSSHALPSGGASWWTTT VPGTTTSPFKLPKRRMKPKLPRPRRSSSKPRRCL RR*MWICRRSCRPCGTAA*VSTSTRSRASRAWR KTSTRR*ASSTRTSMMCWSAWRSNTGATPSRSR PSPVTTRLQKGTRALRLQMAPLPPPPRSESTTSQ SRPAGPRPGPPSPSPHLSQQRPRRWRVGPNLRL EPRSQGRRRQVKQPPALFLLSWWRPSQQL*MAP WRAAVGPGAWTCPQVSCSRYRPSTTTRPLTQTSC SSRLVMWCW*SPSRTLKSRMKAGSWA*RRATGT STRSWRSAVASSPRTSLRGSHDGGAQAASGRVK NTSSRKMCGSFFCFVFVFHLLKSKGKSRGDPQAE GRSPKD*VVFQRAASRQVRRNSPVFLKLLCPLV EFLAPLPVPACVPGRRAGLGAAEPPCLPEASAA PPGQGSSFPGSCCGWGPDTSLAWLCPADGLCAV* K*ILVFKTK*NKKKMIKTL

Frame 2

RPSLLADLAVPAPGVAL*KAAYCPEGAGGGRRP RPEARPLPSPSLCPRVARWLARWLARPSGARSA SVSWLRCRVRGVERQPVWTRGRGWGLGARRARS PRARAAPATGRGLRRDGRDGQ*RGDGGKDRQQRA EEAHPRAGEGSPEAGEGR*DQG*AV*AVRPEFQ QAADGGHPAAEGSPDLPGLRQSHARGFQEAE*V SAGGV*ARLARQG*GKQDRREQRPAVDGLPPEAG GPGAADHGHVPGPVPRHQVTHCQAGAQAGGLRQ CPAPLRVPSNCQKEG*SQNCQGRGGAHQSPEGV *GDECGSAGGAAVPVEQPRRFLRQHVPEHRGPGG KLPQGDEQAQPEPQ*CAGRPGEATREQHLHGQG PAQ*QRACKREQEPFASRWLPCRHPRDQSQPRA RAGRRGHARGHPPQVPISASRGLGGGGWDPTCGW SPGARGDGGK*SSLQLSSCCRGGDLPSNCEWHR GGRQWGRALGPAPRFHVQGTGPARLHGH*HRRA AAQGW*CGAGDPLPEP*RAG*RLAHGREGERLEP AQGAGEVPWRLPRELH*EGPMTAGPRQPPGV*R TPPPEKCVVLFFVLFSFFIF*RAKGNQEETPRQ RGVLPKIRSFSKEPRPGKSGGIHQCS*SCCVL*L SFWRPCLCPHVCLAAGRGWGLPSHHACLKLRPR HPGKGPLFLAAAVGGAQTPA*PGSAPQTVCVLF ENKS*CSKQNETKKK**KLS

Sequence NM_139351

CGCGCCCCTCCCTCCTCGCGGACCTGGCGGTGCCGGCGCCCGGAGTGGCCCTTTAAAAGGCAGCTTATTGTCCGGAGGGGGCGGGCGGGGGGCGCCGACC GCGGCCTGAGGCCCGGCCCCTCCCCTCTCCCTCCCTCTGTCCCCGCGTCGCTCGCTGGCTAGCTCGCTGGCTCGCTCGCCCGTCCGGCGCACGCTCCGCC TCCGTCAGTTGGCTCCGCTGTCGGGTGCGCGGCGTGGAGCGGCAGCCGGTCTGGACGCGCGGCCGGGGCTGGGGGCTGGGAGCGCGGCGCGCAAGATCTC CCCGCGCGAGAGCGGCCCCTGCCACCGGGCGAGGCCTGCGCCGCGATGGCAGAGATGGGCAGTAAAGGGGTGACGGCGGGAAAGATCGCCAGCAACGTGC AGAAGAAGCTCACCCGCGCGCAGGAGAAGGTTCTCCAGAAGCTGGGGAAGGCAGATGAGACCAAGGATGAGCAGTTTGAGCAGTGCGTCCAGAATTTCAA CAAGCAGCTGACGGAGGGCACCCGGCTGCAGAAGGATCTCCGGACCTACCTGGCCTCCGTCAAAGCCATGCACGAGGCTTCCAAGAAGCTGAATGAGTGT CTGCAGGAGGTGTATGAGCCCGATTGGCCCGGCAGGGATGAGGCAAACAAGATCGCAGAGAACAACGACCTGCTGTGGATGGATTACCACCAGAAGCTGG TGGACCAGGCGCTGCTGACCATGGACACGTACCTGGGCCAGTTCCCCGACATCAAGTCACGCATTGCCAAGCGGGGGCGCAAGCTGGTGGACTACGACAG TGCCCGGCACCACTACGAGTCCCTTCAAACTGCCAAAAAGAAGGATGAAGCCAAAATTGCCAAGGCCGAGGAGGAGCTCATCAAAGCCCAGAAGGTGTTT GAGGAGATGAATGTGGATCTGCAGGAGGAGCTGCCGTCCCTGTGGAACAGCCGCGTAGGTTTCTACGTCAACACGTTCCAGAGCATCGCGGGCCTGGAGG AAAACTTCCACAAGGAGATGAGCAAGCTCAACCAGAACCTCAATGATGTGCTGGTCGGCCTGGAGAAGCAACACGGGAGCAACACCTTCACGGTCAAGGC CCAGCCCAGTGACAACGCGCCTGCAAAAGGGAACAAGAGCCCTTCGCCTCCAGATGGCTCCCCTGCCGCCACCCCCGAGATCAGAGTCAACCACGAGCCA GAGCCGGCCGGCGGGGCCACGCCCGGGGCCACCCTCCCCAAGTCCCCATCTCAGAGCTCTCTTCCTGCTGTCGTGGTGGAGACCTTCCCAGCAACTGTGA ATGGCACCGTGGAGGGCGGCAGTGGGGCCGGGCGCTTGGACCTGCCCCCAGGTTTCATGTTCAAGGTACAGGCCCAGCACGACTACACGGCCACTGACAC AGACGAGCTGCAGCTCAAGGCTGGTGATGTGGTGCTGGTGATCCCCTTCCAGAACCCTGAAGAGCAGGATGAAGGCTGGCTCATGGGCGTGAAGGAGAGC GACTGGAACCAGCACAAGGAGCTGGAGAAGTGCCGTGGCGTCTTCCCCGAGAACTTCACTGAGAGGGTCCCATGACGGCGGGGCCCAGGCAGCCTCCGGG CGTGTGAAGAACACCTCCTCCCGAAAAATGTGTGGTTCTTTTTTTTGTTTTGTTTTCGTTTTTCATCTTTTGAAGAGCAAAGGGAAATCAAGAGGAGACC CCCAGGCAGAGGGGCGTTCTCCCAAAGATTAGGTCGTTTTCCAAAGAGCCGCGTCCCGGCAAGTCCGGCGGAATTCACCAGTGTTCCTGAAGCTGCTGTG TCCTCTAGTTGAGTTTCTGGCGCCCCTGCCTGTGCCCGCATGTGTGCCTGGCCGCAGGGCGGGGCTGGGGGCTGCCGAGCCACCATGCTTGCCTGAAGCT TCGGCCGCGCCACCCGGGCAAGGGTCCTCTTTTCCTGGCAGCTGCTGTGGGTGGGGCCCAGACACCAGCCTAGCCTGGCTCTGCCCCGCAGACGGTCTGT GTGCTGTTTGAAAATAAATCTTAGTGTTCAAAACAAAATGAAACAAAAAAAAAATGATAAAAACTCTCAG

refseq_BIN1.F7 (forward primer)
refseq_BIN1.R7 (reverse primer)
Single exon skipping

Frame 0

RAPPSSRTWRCRRPEWPFKRQLIVRRGRAGGAD RGLRPGPSPLPPSVPASLAG*LAGSLARPAHAPP PSVGSAVGCAAWSGSRSGRAAGAGGWERGAQDL PARERPLPPGEACAAMAEMGSKGVTAGKIASNV QKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFN KQLTEGTRLQKDLRTYLASVKAMHEASKKLNEC LQEVYEPDWPGRDEANKIAENNDLLWMDYHQKL VDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDS ARHHYESLQTAKKKDEAKIAKAEEELIKAQKVF EEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLE ENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKA QPSDNAPAKGNKSPSPPDGSPAATPEIRVNHEP EPAGGATPGATLPKSPSQSSLPAVVVETFPATV NGTVEGGSGAGRLDLPPGFMFKVQAQHDYTATDT DELQLKAGDVVLVIPFQNPEEQDEGWLMGVKES DWNQHKELEKCRGVFPENFTERVP*RRGPGSLR ACEEHLLPKNVWFFFLFCFRFSSFEEQREIKRRP PGRGAFSQRLGRFPKSRVPASPAEFTSVPEAAV SSS*VSGAPACARMCAWPQGGAGGCRATMLA*S FGRATRARVLFSWQLLWVGPRHQPSLALPRRRSV CCLKINLSVQNKMKQKKNDKNSQ

Frame 1

APLPPRGPGGAGARSGPLKGSLLSGGGGRGAPT AA*GPAPPLSLPLSPRRSLASSLARSPVRRTLR LRQLAPLSGARRGAAAGLDARPGLGAGSAARKIS PRESGPCHRARPAPRWQRWAVKG*RRERSPATC RRSSPARRRRFSRSWGRQMRPRMSSLSSASRIS TSS*RRAPGCRRISGPTWPPSKPCTRLPRS*MSV CRRCMSPIGPAGMRQTRSQRTTTCCGWITTRSW WTRRC*PWTRTWASSPTSSHALPSGGASWWTTT VPGTTTSPFKLPKRRMKPKLPRPRRSSSKPRRCL RR*MWICRRSCRPCGTAA*VSTSTRSRASRAWR KTSTRR*ASSTRTSMMCWSAWRSNTGATPSRSR PSPVTTRLQKGTRALRLQMAPLPPPPRSESTTSQ SRPAGPRPGPPSPSPHLRALFLLSWWRPSQQL* MAPWRAAVGPGAWTCPQVSCSRYRPSTTTRPLT QTSCSSRLVMWCW*SPSRTLKSRMKAGSWA*RRA TGTSTRSWRSAVASSPRTSLRGSHDGGAQAASG RVKNTSSRKMCGSFFCFVFVFHLLKSKGKSRGD PQAEGRSPKD*VVFQRAASRQVRRNSPVFLKLLC PLVEFLAPLPVPACVPGRRAGLGAAEPPCLPEA SAAPPGQGSSFPGSCCGWGPDTSLAWLCPADGL CAV*K*ILVFKTK*NKKKMIKTL

Frame 2

RPSLLADLAVPAPGVAL*KAAYCPEGAGGGRRP RPEARPLPSPSLCPRVARWLARWLARPSGARSA SVSWLRCRVRGVERQPVWTRGRGWGLGARRARS PRARAAPATGRGLRRDGRDGQ*RGDGGKDRQQRA EEAHPRAGEGSPEAGEGR*DQG*AV*AVRPEFQ QAADGGHPAAEGSPDLPGLRQSHARGFQEAE*V SAGGV*ARLARQG*GKQDRREQRPAVDGLPPEAG GPGAADHGHVPGPVPRHQVTHCQAGAQAGGLRQ CPAPLRVPSNCQKEG*SQNCQGRGGAHQSPEGV *GDECGSAGGAAVPVEQPRRFLRQHVPEHRGPGG KLPQGDEQAQPEPQ*CAGRPGEATREQHLHGQG PAQ*QRACKREQEPFASRWLPCRHPRDQSQPRA RAGRRGHARGHPPQVPISELSSCCRGGDLPSNCE WHRGGRQWGRALGPAPRFHVQGTGPARLHGH*H RRAAAQGW*CGAGDPLPEP*RAG*RLAHGREGE RLEPAQGAGEVPWRLPRELH*EGPMTAGPRQPPG V*RTPPPEKCVVLFFVLFSFFIF*RAKGNQEET PRQRGVLPKIRSFSKEPRPGKSGGIHQCS*SCC VL*LSFWRPCLCPHVCLAAGRGWGLPSHHACLKL RPRHPGKGPLFLAAAVGGAQTPA*PGSAPQTVC VLFENKS*CSKQNETKKK**KLS

Sequence NM_139344

CGCGCCCCTCCCTCCTCGCGGACCTGGCGGTGCCGGCGCCCGGAGTGGCCCTTTAAAAGGCAGCTTATTGTCCGGAGGGGGCGGGCGGGGGGCGCCGACC GCGGCCTGAGGCCCGGCCCCTCCCCTCTCCCTCCCTCTGTCCCCGCGTCGCTCGCTGGCTAGCTCGCTGGCTCGCTCGCCCGTCCGGCGCACGCTCCGCC TCCGTCAGTTGGCTCCGCTGTCGGGTGCGCGGCGTGGAGCGGCAGCCGGTCTGGACGCGCGGCCGGGGCTGGGGGCTGGGAGCGCGGCGCGCAAGATCTC CCCGCGCGAGAGCGGCCCCTGCCACCGGGCGAGGCCTGCGCCGCGATGGCAGAGATGGGCAGTAAAGGGGTGACGGCGGGAAAGATCGCCAGCAACGTGC AGAAGAAGCTCACCCGCGCGCAGGAGAAGGTTCTCCAGAAGCTGGGGAAGGCAGATGAGACCAAGGATGAGCAGTTTGAGCAGTGCGTCCAGAATTTCAA CAAGCAGCTGACGGAGGGCACCCGGCTGCAGAAGGATCTCCGGACCTACCTGGCCTCCGTCAAAGCCATGCACGAGGCTTCCAAGAAGCTGAATGAGTGT CTGCAGGAGGTGTATGAGCCCGATTGGCCCGGCAGGGATGAGGCAAACAAGATCGCAGAGAACAACGACCTGCTGTGGATGGATTACCACCAGAAGCTGG TGGACCAGGCGCTGCTGACCATGGACACGTACCTGGGCCAGTTCCCCGACATCAAGTCACGCATTGCCAAGCGGGGGCGCAAGCTGGTGGACTACGACAG TGCCCGGCACCACTACGAGTCCCTTCAAACTGCCAAAAAGAAGGATGAAGCCAAAATTGCCAAGCCTGTCTCGCTGCTTGAGAAAGCCGCCCCCCAGTGG TGCCAAGGCAAACTGCAGGCTCATCTCGTAGCTCAAACTAACCTGCTCCGAAATCAGGCCGAGGAGGAGCTCATCAAAGCCCAGAAGGTGTTTGAGGAGA TGAATGTGGATCTGCAGGAGGAGCTGCCGTCCCTGTGGAACAGCCGCGTAGGTTTCTACGTCAACACGTTCCAGAGCATCGCGGGCCTGGAGGAAAACTT CCACAAGGAGATGAGCAAGCTCAACCAGAACCTCAATGATGTGCTGGTCGGCCTGGAGAAGCAACACGGGAGCAACACCTTCACGGTCAAGGCCCAGCCC AGTGACAACGCGCCTGCAAAAGGGAACAAGAGCCCTTCGCCTCCAGATGGCTCCCCTGCCGCCACCCCCGAGATCAGAGTCAACCACGAGCCAGAGCCGG CCGGCGGGGCCACGCCCGGGGCCACCCTCCCCAAGTCCCCATCTCAGTTTGAGGCCCCGGGGCCTTTCTCGGAGCAGGCCAGTCTGCTGGACCTGGACTT TGACCCCCTCCCGCCCGTGACGAGCCCTGTGAAGGCACCCACGCCCTCTGGTCAGTCAATTCCATGGGACCTCTGGGAGCCCACAGAGAGTCCAGCCGGC AGCCTGCCTTCCGGGGAGCCCAGCGCTGCCGAGGGCACCTTTGCTGTGTCCTGGCCCAGCCAGACGGCCGAGCCGGGGCCTGCCCAACCAGCAGAGGCCT CGGAGGTGGCGGGTGGGACCCAACCTGCGGCTGGAGCCCAGGAGCCAGGGGAGACGGCGGCAAGTGAAGCAGCCTCCAGCTCTCTTCCTGCTGTCGTGGT GGAGACCTTCCCAGCAACTGTGAATGGCACCGTGGAGGGCGGCAGTGGGGCCGGGCGCTTGGACCTGCCCCCAGGTTTCATGTTCAAGGTACAGGCCCAG CACGACTACACGGCCACTGACACAGACGAGCTGCAGCTCAAGGCTGGTGATGTGGTGCTGGTGATCCCCTTCCAGAACCCTGAAGAGCAGGATGAAGGCT GGCTCATGGGCGTGAAGGAGAGCGACTGGAACCAGCACAAGGAGCTGGAGAAGTGCCGTGGCGTCTTCCCCGAGAACTTCACTGAGAGGGTCCCATGACG GCGGGGCCCAGGCAGCCTCCGGGCGTGTGAAGAACACCTCCTCCCGAAAAATGTGTGGTTCTTTTTTTTGTTTTGTTTTCGTTTTTCATCTTTTGAAGAG CAAAGGGAAATCAAGAGGAGACCCCCAGGCAGAGGGGCGTTCTCCCAAAGATTAGGTCGTTTTCCAAAGAGCCGCGTCCCGGCAAGTCCGGCGGAATTCA CCAGTGTTCCTGAAGCTGCTGTGTCCTCTAGTTGAGTTTCTGGCGCCCCTGCCTGTGCCCGCATGTGTGCCTGGCCGCAGGGCGGGGCTGGGGGCTGCCG AGCCACCATGCTTGCCTGAAGCTTCGGCCGCGCCACCCGGGCAAGGGTCCTCTTTTCCTGGCAGCTGCTGTGGGTGGGGCCCAGACACCAGCCTAGCCTG GCTCTGCCCCGCAGACGGTCTGTGTGCTGTTTGAAAATAAATCTTAGTGTTCAAAACAAAATGAAACAAAAAAAAAATGATAAAAACTCTCAG

refseq_BIN1.F7 (forward primer)
refseq_BIN1.R7 (reverse primer)
Single exon skipping

Frame 0

RAPPSSRTWRCRRPEWPFKRQLIVRRGRAGGAD RGLRPGPSPLPPSVPASLAG*LAGSLARPAHAPP PSVGSAVGCAAWSGSRSGRAAGAGGWERGAQDL PARERPLPPGEACAAMAEMGSKGVTAGKIASNV QKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFN KQLTEGTRLQKDLRTYLASVKAMHEASKKLNEC LQEVYEPDWPGRDEANKIAENNDLLWMDYHQKL VDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDS ARHHYESLQTAKKKDEAKIAKPVSLLEKAAPQW CQGKLQAHLVAQTNLLRNQAEEELIKAQKVFEE MNVDLQEELPSLWNSRVGFYVNTFQSIAGLEENF HKEMSKLNQNLNDVLVGLEKQHGSNTFTVKAQP SDNAPAKGNKSPSPPDGSPAATPEIRVNHEPEP AGGATPGATLPKSPSQFEAPGPFSEQASLLDLDF DPLPPVTSPVKAPTPSGQSIPWDLWEPTESPAG SLPSGEPSAAEGTFAVSWPSQTAEPGPAQPAEA SEVAGGTQPAAGAQEPGETAASEAASSSLPAVVV ETFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQ HDYTATDTDELQLKAGDVVLVIPFQNPEEQDEG WLMGVKESDWNQHKELEKCRGVFPENFTERVP*R RGPGSLRACEEHLLPKNVWFFFLFCFRFSSFEE QREIKRRPPGRGAFSQRLGRFPKSRVPASPAEF TSVPEAAVSSS*VSGAPACARMCAWPQGGAGGCR ATMLA*SFGRATRARVLFSWQLLWVGPRHQPSL ALPRRRSVCCLKINLSVQNKMKQKKNDKNSQ

Frame 1

APLPPRGPGGAGARSGPLKGSLLSGGGGRGAPT AA*GPAPPLSLPLSPRRSLASSLARSPVRRTLR LRQLAPLSGARRGAAAGLDARPGLGAGSAARKIS PRESGPCHRARPAPRWQRWAVKG*RRERSPATC RRSSPARRRRFSRSWGRQMRPRMSSLSSASRIS TSS*RRAPGCRRISGPTWPPSKPCTRLPRS*MSV CRRCMSPIGPAGMRQTRSQRTTTCCGWITTRSW WTRRC*PWTRTWASSPTSSHALPSGGASWWTTT VPGTTTSPFKLPKRRMKPKLPSLSRCLRKPPPSG AKANCRLIS*LKLTCSEIRPRRSSSKPRRCLRR *MWICRRSCRPCGTAA*VSTSTRSRASRAWRKT STRR*ASSTRTSMMCWSAWRSNTGATPSRSRPSP VTTRLQKGTRALRLQMAPLPPPPRSESTTSQSR PAGPRPGPPSPSPHLSLRPRGLSRSRPVCWTWT LTPSRP*RAL*RHPRPLVSQFHGTSGSPQRVQPA ACLPGSPALPRAPLLCPGPARRPSRGLPNQQRP RRWRVGPNLRLEPRSQGRRRQVKQPPALFLLSW WRPSQQL*MAPWRAAVGPGAWTCPQVSCSRYRPS TTTRPLTQTSCSSRLVMWCW*SPSRTLKSRMKA GSWA*RRATGTSTRSWRSAVASSPRTSLRGSHD GGAQAASGRVKNTSSRKMCGSFFCFVFVFHLLKS KGKSRGDPQAEGRSPKD*VVFQRAASRQVRRNS PVFLKLLCPLVEFLAPLPVPACVPGRRAGLGAA EPPCLPEASAAPPGQGSSFPGSCCGWGPDTSLAW LCPADGLCAV*K*ILVFKTK*NKKKMIKTL

Frame 2

RPSLLADLAVPAPGVAL*KAAYCPEGAGGGRRP RPEARPLPSPSLCPRVARWLARWLARPSGARSA SVSWLRCRVRGVERQPVWTRGRGWGLGARRARS PRARAAPATGRGLRRDGRDGQ*RGDGGKDRQQRA EEAHPRAGEGSPEAGEGR*DQG*AV*AVRPEFQ QAADGGHPAAEGSPDLPGLRQSHARGFQEAE*V SAGGV*ARLARQG*GKQDRREQRPAVDGLPPEAG GPGAADHGHVPGPVPRHQVTHCQAGAQAGGLRQ CPAPLRVPSNCQKEG*SQNCQACLAA*ESRPPV VPRQTAGSSRSSN*PAPKSGRGGAHQSPEGV*GD ECGSAGGAAVPVEQPRRFLRQHVPEHRGPGGKL PQGDEQAQPEPQ*CAGRPGEATREQHLHGQGPA Q*QRACKREQEPFASRWLPCRHPRDQSQPRARAG RRGHARGHPPQVPISV*GPGAFLGAGQSAGPGL *PPPARDEPCEGTHALWSVNSMGPLGAHRESSR QPAFRGAQRCRGHLCCVLAQPDGRAGACPTSRGL GGGGWDPTCGWSPGARGDGGK*SSLQLSSCCRG GDLPSNCEWHRGGRQWGRALGPAPRFHVQGTGP ARLHGH*HRRAAAQGW*CGAGDPLPEP*RAG*RL AHGREGERLEPAQGAGEVPWRLPRELH*EGPMT AGPRQPPGV*RTPPPEKCVVLFFVLFSFFIF*R AKGNQEETPRQRGVLPKIRSFSKEPRPGKSGGIH QCS*SCCVL*LSFWRPCLCPHVCLAAGRGWGLP SHHACLKLRPRHPGKGPLFLAAAVGGAQTPA*P GSAPQTVCVLFENKS*CSKQNETKKK**KLS

Sequence NM_139343

CGCGCCCCTCCCTCCTCGCGGACCTGGCGGTGCCGGCGCCCGGAGTGGCCCTTTAAAAGGCAGCTTATTGTCCGGAGGGGGCGGGCGGGGGGCGCCGACC GCGGCCTGAGGCCCGGCCCCTCCCCTCTCCCTCCCTCTGTCCCCGCGTCGCTCGCTGGCTAGCTCGCTGGCTCGCTCGCCCGTCCGGCGCACGCTCCGCC TCCGTCAGTTGGCTCCGCTGTCGGGTGCGCGGCGTGGAGCGGCAGCCGGTCTGGACGCGCGGCCGGGGCTGGGGGCTGGGAGCGCGGCGCGCAAGATCTC CCCGCGCGAGAGCGGCCCCTGCCACCGGGCGAGGCCTGCGCCGCGATGGCAGAGATGGGCAGTAAAGGGGTGACGGCGGGAAAGATCGCCAGCAACGTGC AGAAGAAGCTCACCCGCGCGCAGGAGAAGGTTCTCCAGAAGCTGGGGAAGGCAGATGAGACCAAGGATGAGCAGTTTGAGCAGTGCGTCCAGAATTTCAA CAAGCAGCTGACGGAGGGCACCCGGCTGCAGAAGGATCTCCGGACCTACCTGGCCTCCGTCAAAGCCATGCACGAGGCTTCCAAGAAGCTGAATGAGTGT CTGCAGGAGGTGTATGAGCCCGATTGGCCCGGCAGGGATGAGGCAAACAAGATCGCAGAGAACAACGACCTGCTGTGGATGGATTACCACCAGAAGCTGG TGGACCAGGCGCTGCTGACCATGGACACGTACCTGGGCCAGTTCCCCGACATCAAGTCACGCATTGCCAAGCGGGGGCGCAAGCTGGTGGACTACGACAG TGCCCGGCACCACTACGAGTCCCTTCAAACTGCCAAAAAGAAGGATGAAGCCAAAATTGCCAAGCCTGTCTCGCTGCTTGAGAAAGCCGCCCCCCAGTGG TGCCAAGGCAAACTGCAGGCTCATCTCGTAGCTCAAACTAACCTGCTCCGAAATCAGGCCGAGGAGGAGCTCATCAAAGCCCAGAAGGTGTTTGAGGAGA TGAATGTGGATCTGCAGGAGGAGCTGCCGTCCCTGTGGAACAGCCGCGTAGGTTTCTACGTCAACACGTTCCAGAGCATCGCGGGCCTGGAGGAAAACTT CCACAAGGAGATGAGCAAGCTCAACCAGAACCTCAATGATGTGCTGGTCGGCCTGGAGAAGCAACACGGGAGCAACACCTTCACGGTCAAGGCCCAGCCC AGTGACAACGCGCCTGCAAAAGGGAACAAGAGCCCTTCGCCTCCAGATGGCTCCCCTGCCGCCACCCCCGAGATCAGAGTCAACCACGAGCCAGAGCCGG CCGGCGGGGCCACGCCCGGGGCCACCCTCCCCAAGTCCCCATCTCAGCTCCGGAAAGGCCCACCAGTCCCTCCGCCTCCCAAACACACCCCGTCCAAGGA AGTCAAGCAGGAGCAGATCCTCAGCCTGTTTGAGGACACGTTTGTCCCTGAGATCAGCGTGACCACCCCCTCCCAGTTTGAGGCCCCGGGGCCTTTCTCG GAGCAGGCCAGTCTGCTGGACCTGGACTTTGACCCCCTCCCGCCCGTGACGAGCCCTGTGAAGGCACCCACGCCCTCTGGTCAGTCAATTCCATGGGACC TCTGGGAGCCCACAGAGAGTCCAGCCGGCAGCCTGCCTTCCGGGGAGCCCAGCGCTGCCGAGGGCACCTTTGCTGTGTCCTGGCCCAGCCAGACGGCCGA GCCGGGGCCTGCCCAACCAGCAGAGGCCTCGGAGGTGGCGGGTGGGACCCAACCTGCGGCTGGAGCCCAGGAGCCAGGGGAGACGGCGGCAAGTGAAGCA GCCTCCAGCTCTCTTCCTGCTGTCGTGGTGGAGACCTTCCCAGCAACTGTGAATGGCACCGTGGAGGGCGGCAGTGGGGCCGGGCGCTTGGACCTGCCCC CAGGTTTCATGTTCAAGGTACAGGCCCAGCACGACTACACGGCCACTGACACAGACGAGCTGCAGCTCAAGGCTGGTGATGTGGTGCTGGTGATCCCCTT CCAGAACCCTGAAGAGCAGGATGAAGGCTGGCTCATGGGCGTGAAGGAGAGCGACTGGAACCAGCACAAGGAGCTGGAGAAGTGCCGTGGCGTCTTCCCC GAGAACTTCACTGAGAGGGTCCCATGACGGCGGGGCCCAGGCAGCCTCCGGGCGTGTGAAGAACACCTCCTCCCGAAAAATGTGTGGTTCTTTTTTTTGT TTTGTTTTCGTTTTTCATCTTTTGAAGAGCAAAGGGAAATCAAGAGGAGACCCCCAGGCAGAGGGGCGTTCTCCCAAAGATTAGGTCGTTTTCCAAAGAG CCGCGTCCCGGCAAGTCCGGCGGAATTCACCAGTGTTCCTGAAGCTGCTGTGTCCTCTAGTTGAGTTTCTGGCGCCCCTGCCTGTGCCCGCATGTGTGCC TGGCCGCAGGGCGGGGCTGGGGGCTGCCGAGCCACCATGCTTGCCTGAAGCTTCGGCCGCGCCACCCGGGCAAGGGTCCTCTTTTCCTGGCAGCTGCTGT GGGTGGGGCCCAGACACCAGCCTAGCCTGGCTCTGCCCCGCAGACGGTCTGTGTGCTGTTTGAAAATAAATCTTAGTGTTCAAAACAAAATGAAACAAAA AAAAAATGATAAAAACTCTCAG

refseq_BIN1.F7 (forward primer)
refseq_BIN1.R7 (reverse primer)
Single exon skipping

Frame 0

RAPPSSRTWRCRRPEWPFKRQLIVRRGRAGGAD RGLRPGPSPLPPSVPASLAG*LAGSLARPAHAPP PSVGSAVGCAAWSGSRSGRAAGAGGWERGAQDL PARERPLPPGEACAAMAEMGSKGVTAGKIASNV QKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFN KQLTEGTRLQKDLRTYLASVKAMHEASKKLNEC LQEVYEPDWPGRDEANKIAENNDLLWMDYHQKL VDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDS ARHHYESLQTAKKKDEAKIAKPVSLLEKAAPQW CQGKLQAHLVAQTNLLRNQAEEELIKAQKVFEE MNVDLQEELPSLWNSRVGFYVNTFQSIAGLEENF HKEMSKLNQNLNDVLVGLEKQHGSNTFTVKAQP SDNAPAKGNKSPSPPDGSPAATPEIRVNHEPEP AGGATPGATLPKSPSQLRKGPPVPPPPKHTPSKE VKQEQILSLFEDTFVPEISVTTPSQFEAPGPFS EQASLLDLDFDPLPPVTSPVKAPTPSGQSIPWD LWEPTESPAGSLPSGEPSAAEGTFAVSWPSQTAE PGPAQPAEASEVAGGTQPAAGAQEPGETAASEA ASSSLPAVVVETFPATVNGTVEGGSGAGRLDLP PGFMFKVQAQHDYTATDTDELQLKAGDVVLVIPF QNPEEQDEGWLMGVKESDWNQHKELEKCRGVFP ENFTERVP*RRGPGSLRACEEHLLPKNVWFFFL FCFRFSSFEEQREIKRRPPGRGAFSQRLGRFPKS RVPASPAEFTSVPEAAVSSS*VSGAPACARMCA WPQGGAGGCRATMLA*SFGRATRARVLFSWQLL WVGPRHQPSLALPRRRSVCCLKINLSVQNKMKQK KNDKNSQ

Frame 1

APLPPRGPGGAGARSGPLKGSLLSGGGGRGAPT AA*GPAPPLSLPLSPRRSLASSLARSPVRRTLR LRQLAPLSGARRGAAAGLDARPGLGAGSAARKIS PRESGPCHRARPAPRWQRWAVKG*RRERSPATC RRSSPARRRRFSRSWGRQMRPRMSSLSSASRIS TSS*RRAPGCRRISGPTWPPSKPCTRLPRS*MSV CRRCMSPIGPAGMRQTRSQRTTTCCGWITTRSW WTRRC*PWTRTWASSPTSSHALPSGGASWWTTT VPGTTTSPFKLPKRRMKPKLPSLSRCLRKPPPSG AKANCRLIS*LKLTCSEIRPRRSSSKPRRCLRR *MWICRRSCRPCGTAA*VSTSTRSRASRAWRKT STRR*ASSTRTSMMCWSAWRSNTGATPSRSRPSP VTTRLQKGTRALRLQMAPLPPPPRSESTTSQSR PAGPRPGPPSPSPHLSSGKAHQSLRLPNTPRPR KSSRSRSSACLRTRLSLRSA*PPPPSLRPRGLSR SRPVCWTWTLTPSRP*RAL*RHPRPLVSQFHGT SGSPQRVQPAACLPGSPALPRAPLLCPGPARRP SRGLPNQQRPRRWRVGPNLRLEPRSQGRRRQVKQ PPALFLLSWWRPSQQL*MAPWRAAVGPGAWTCP QVSCSRYRPSTTTRPLTQTSCSSRLVMWCW*SP SRTLKSRMKAGSWA*RRATGTSTRSWRSAVASSP RTSLRGSHDGGAQAASGRVKNTSSRKMCGSFFC FVFVFHLLKSKGKSRGDPQAEGRSPKD*VVFQR AASRQVRRNSPVFLKLLCPLVEFLAPLPVPACVP GRRAGLGAAEPPCLPEASAAPPGQGSSFPGSCC GWGPDTSLAWLCPADGLCAV*K*ILVFKTK*NK KKMIKTL

Frame 2

RPSLLADLAVPAPGVAL*KAAYCPEGAGGGRRP RPEARPLPSPSLCPRVARWLARWLARPSGARSA SVSWLRCRVRGVERQPVWTRGRGWGLGARRARS PRARAAPATGRGLRRDGRDGQ*RGDGGKDRQQRA EEAHPRAGEGSPEAGEGR*DQG*AV*AVRPEFQ QAADGGHPAAEGSPDLPGLRQSHARGFQEAE*V SAGGV*ARLARQG*GKQDRREQRPAVDGLPPEAG GPGAADHGHVPGPVPRHQVTHCQAGAQAGGLRQ CPAPLRVPSNCQKEG*SQNCQACLAA*ESRPPV VPRQTAGSSRSSN*PAPKSGRGGAHQSPEGV*GD ECGSAGGAAVPVEQPRRFLRQHVPEHRGPGGKL PQGDEQAQPEPQ*CAGRPGEATREQHLHGQGPA Q*QRACKREQEPFASRWLPCRHPRDQSQPRARAG RRGHARGHPPQVPISAPERPTSPSASQTHPVQG SQAGADPQPV*GHVCP*DQRDHPLPV*GPGAFL GAGQSAGPGL*PPPARDEPCEGTHALWSVNSMGP LGAHRESSRQPAFRGAQRCRGHLCCVLAQPDGR AGACPTSRGLGGGGWDPTCGWSPGARGDGGK*S SLQLSSCCRGGDLPSNCEWHRGGRQWGRALGPAP RFHVQGTGPARLHGH*HRRAAAQGW*CGAGDPL PEP*RAG*RLAHGREGERLEPAQGAGEVPWRLP RELH*EGPMTAGPRQPPGV*RTPPPEKCVVLFFV LFSFFIF*RAKGNQEETPRQRGVLPKIRSFSKE PRPGKSGGIHQCS*SCCVL*LSFWRPCLCPHVC LAAGRGWGLPSHHACLKLRPRHPGKGPLFLAAAV GGAQTPA*PGSAPQTVCVLFENKS*CSKQNETK KK**KLS

Sequence NM_139348

CGCGCCCCTCCCTCCTCGCGGACCTGGCGGTGCCGGCGCCCGGAGTGGCCCTTTAAAAGGCAGCTTATTGTCCGGAGGGGGCGGGCGGGGGGCGCCGACC GCGGCCTGAGGCCCGGCCCCTCCCCTCTCCCTCCCTCTGTCCCCGCGTCGCTCGCTGGCTAGCTCGCTGGCTCGCTCGCCCGTCCGGCGCACGCTCCGCC TCCGTCAGTTGGCTCCGCTGTCGGGTGCGCGGCGTGGAGCGGCAGCCGGTCTGGACGCGCGGCCGGGGCTGGGGGCTGGGAGCGCGGCGCGCAAGATCTC CCCGCGCGAGAGCGGCCCCTGCCACCGGGCGAGGCCTGCGCCGCGATGGCAGAGATGGGCAGTAAAGGGGTGACGGCGGGAAAGATCGCCAGCAACGTGC AGAAGAAGCTCACCCGCGCGCAGGAGAAGGTTCTCCAGAAGCTGGGGAAGGCAGATGAGACCAAGGATGAGCAGTTTGAGCAGTGCGTCCAGAATTTCAA CAAGCAGCTGACGGAGGGCACCCGGCTGCAGAAGGATCTCCGGACCTACCTGGCCTCCGTCAAAGCCATGCACGAGGCTTCCAAGAAGCTGAATGAGTGT CTGCAGGAGGTGTATGAGCCCGATTGGCCCGGCAGGGATGAGGCAAACAAGATCGCAGAGAACAACGACCTGCTGTGGATGGATTACCACCAGAAGCTGG TGGACCAGGCGCTGCTGACCATGGACACGTACCTGGGCCAGTTCCCCGACATCAAGTCACGCATTGCCAAGCGGGGGCGCAAGCTGGTGGACTACGACAG TGCCCGGCACCACTACGAGTCCCTTCAAACTGCCAAAAAGAAGGATGAAGCCAAAATTGCCAAGGCCGAGGAGGAGCTCATCAAAGCCCAGAAGGTGTTT GAGGAGATGAATGTGGATCTGCAGGAGGAGCTGCCGTCCCTGTGGAACAGCCGCGTAGGTTTCTACGTCAACACGTTCCAGAGCATCGCGGGCCTGGAGG AAAACTTCCACAAGGAGATGAGCAAGCTCAACCAGAACCTCAATGATGTGCTGGTCGGCCTGGAGAAGCAACACGGGAGCAACACCTTCACGGTCAAGGC CCAGCCCAGTGACAACGCGCCTGCAAAAGGGAACAAGAGCCCTTCGCCTCCAGATGGCTCCCCTGCCGCCACCCCCGAGATCAGAGTCAACCACGAGCCA GAGCCGGCCGGCGGGGCCACGCCCGGGGCCACCCTCCCCAAGTCCCCATCTCAGCTCCGGAAAGGCCCACCAGTCCCTCCGCCTCCCAAACACACCCCGT CCAAGGAAGTCAAGCAGGAGCAGATCCTCAGCCTGTTTGAGGACACGTTTGTCCCTGAGATCAGCGTGACCACCCCCTCCCAGCCAGCAGAGGCCTCGGA GGTGGCGGGTGGGACCCAACCTGCGGCTGGAGCCCAGGAGCCAGGGGAGACGGCGGCAAGTGAAGCAGCCTCCAGCTCTCTTCCTGCTGTCGTGGTGGAG ACCTTCCCAGCAACTGTGAATGGCACCGTGGAGGGCGGCAGTGGGGCCGGGCGCTTGGACCTGCCCCCAGGTTTCATGTTCAAGGTACAGGCCCAGCACG ACTACACGGCCACTGACACAGACGAGCTGCAGCTCAAGGCTGGTGATGTGGTGCTGGTGATCCCCTTCCAGAACCCTGAAGAGCAGGATGAAGGCTGGCT CATGGGCGTGAAGGAGAGCGACTGGAACCAGCACAAGGAGCTGGAGAAGTGCCGTGGCGTCTTCCCCGAGAACTTCACTGAGAGGGTCCCATGACGGCGG GGCCCAGGCAGCCTCCGGGCGTGTGAAGAACACCTCCTCCCGAAAAATGTGTGGTTCTTTTTTTTGTTTTGTTTTCGTTTTTCATCTTTTGAAGAGCAAA GGGAAATCAAGAGGAGACCCCCAGGCAGAGGGGCGTTCTCCCAAAGATTAGGTCGTTTTCCAAAGAGCCGCGTCCCGGCAAGTCCGGCGGAATTCACCAG TGTTCCTGAAGCTGCTGTGTCCTCTAGTTGAGTTTCTGGCGCCCCTGCCTGTGCCCGCATGTGTGCCTGGCCGCAGGGCGGGGCTGGGGGCTGCCGAGCC ACCATGCTTGCCTGAAGCTTCGGCCGCGCCACCCGGGCAAGGGTCCTCTTTTCCTGGCAGCTGCTGTGGGTGGGGCCCAGACACCAGCCTAGCCTGGCTC TGCCCCGCAGACGGTCTGTGTGCTGTTTGAAAATAAATCTTAGTGTTCAAAACAAAATGAAACAAAAAAAAAATGATAAAAACTCTCAG

refseq_BIN1.F7 (forward primer)
refseq_BIN1.R7 (reverse primer)
Single exon skipping

Frame 0

RAPPSSRTWRCRRPEWPFKRQLIVRRGRAGGAD RGLRPGPSPLPPSVPASLAG*LAGSLARPAHAPP PSVGSAVGCAAWSGSRSGRAAGAGGWERGAQDL PARERPLPPGEACAAMAEMGSKGVTAGKIASNV QKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFN KQLTEGTRLQKDLRTYLASVKAMHEASKKLNEC LQEVYEPDWPGRDEANKIAENNDLLWMDYHQKL VDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDS ARHHYESLQTAKKKDEAKIAKAEEELIKAQKVF EEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLE ENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKA QPSDNAPAKGNKSPSPPDGSPAATPEIRVNHEP EPAGGATPGATLPKSPSQLRKGPPVPPPPKHTP SKEVKQEQILSLFEDTFVPEISVTTPSQPAEASE VAGGTQPAAGAQEPGETAASEAASSSLPAVVVE TFPATVNGTVEGGSGAGRLDLPPGFMFKVQAQH DYTATDTDELQLKAGDVVLVIPFQNPEEQDEGWL MGVKESDWNQHKELEKCRGVFPENFTERVP*RR GPGSLRACEEHLLPKNVWFFFLFCFRFSSFEEQ REIKRRPPGRGAFSQRLGRFPKSRVPASPAEFTS VPEAAVSSS*VSGAPACARMCAWPQGGAGGCRA TMLA*SFGRATRARVLFSWQLLWVGPRHQPSLA LPRRRSVCCLKINLSVQNKMKQKKNDKNSQ

Frame 1

APLPPRGPGGAGARSGPLKGSLLSGGGGRGAPT AA*GPAPPLSLPLSPRRSLASSLARSPVRRTLR LRQLAPLSGARRGAAAGLDARPGLGAGSAARKIS PRESGPCHRARPAPRWQRWAVKG*RRERSPATC RRSSPARRRRFSRSWGRQMRPRMSSLSSASRIS TSS*RRAPGCRRISGPTWPPSKPCTRLPRS*MSV CRRCMSPIGPAGMRQTRSQRTTTCCGWITTRSW WTRRC*PWTRTWASSPTSSHALPSGGASWWTTT VPGTTTSPFKLPKRRMKPKLPRPRRSSSKPRRCL RR*MWICRRSCRPCGTAA*VSTSTRSRASRAWR KTSTRR*ASSTRTSMMCWSAWRSNTGATPSRSR PSPVTTRLQKGTRALRLQMAPLPPPPRSESTTSQ SRPAGPRPGPPSPSPHLSSGKAHQSLRLPNTPR PRKSSRSRSSACLRTRLSLRSA*PPPPSQQRPR RWRVGPNLRLEPRSQGRRRQVKQPPALFLLSWWR PSQQL*MAPWRAAVGPGAWTCPQVSCSRYRPST TTRPLTQTSCSSRLVMWCW*SPSRTLKSRMKAG SWA*RRATGTSTRSWRSAVASSPRTSLRGSHDGG AQAASGRVKNTSSRKMCGSFFCFVFVFHLLKSK GKSRGDPQAEGRSPKD*VVFQRAASRQVRRNSP VFLKLLCPLVEFLAPLPVPACVPGRRAGLGAAEP PCLPEASAAPPGQGSSFPGSCCGWGPDTSLAWL CPADGLCAV*K*ILVFKTK*NKKKMIKTL

Frame 2

RPSLLADLAVPAPGVAL*KAAYCPEGAGGGRRP RPEARPLPSPSLCPRVARWLARWLARPSGARSA SVSWLRCRVRGVERQPVWTRGRGWGLGARRARS PRARAAPATGRGLRRDGRDGQ*RGDGGKDRQQRA EEAHPRAGEGSPEAGEGR*DQG*AV*AVRPEFQ QAADGGHPAAEGSPDLPGLRQSHARGFQEAE*V SAGGV*ARLARQG*GKQDRREQRPAVDGLPPEAG GPGAADHGHVPGPVPRHQVTHCQAGAQAGGLRQ CPAPLRVPSNCQKEG*SQNCQGRGGAHQSPEGV *GDECGSAGGAAVPVEQPRRFLRQHVPEHRGPGG KLPQGDEQAQPEPQ*CAGRPGEATREQHLHGQG PAQ*QRACKREQEPFASRWLPCRHPRDQSQPRA RAGRRGHARGHPPQVPISAPERPTSPSASQTHPV QGSQAGADPQPV*GHVCP*DQRDHPLPASRGLG GGGWDPTCGWSPGARGDGGK*SSLQLSSCCRGG DLPSNCEWHRGGRQWGRALGPAPRFHVQGTGPAR LHGH*HRRAAAQGW*CGAGDPLPEP*RAG*RLA HGREGERLEPAQGAGEVPWRLPRELH*EGPMTA GPRQPPGV*RTPPPEKCVVLFFVLFSFFIF*RAK GNQEETPRQRGVLPKIRSFSKEPRPGKSGGIHQ CS*SCCVL*LSFWRPCLCPHVCLAAGRGWGLPS HHACLKLRPRHPGKGPLFLAAAVGGAQTPA*PGS APQTVCVLFENKS*CSKQNETKKK**KLS

Sequence NM_139349

CGCGCCCCTCCCTCCTCGCGGACCTGGCGGTGCCGGCGCCCGGAGTGGCCCTTTAAAAGGCAGCTTATTGTCCGGAGGGGGCGGGCGGGGGGCGCCGACC GCGGCCTGAGGCCCGGCCCCTCCCCTCTCCCTCCCTCTGTCCCCGCGTCGCTCGCTGGCTAGCTCGCTGGCTCGCTCGCCCGTCCGGCGCACGCTCCGCC TCCGTCAGTTGGCTCCGCTGTCGGGTGCGCGGCGTGGAGCGGCAGCCGGTCTGGACGCGCGGCCGGGGCTGGGGGCTGGGAGCGCGGCGCGCAAGATCTC CCCGCGCGAGAGCGGCCCCTGCCACCGGGCGAGGCCTGCGCCGCGATGGCAGAGATGGGCAGTAAAGGGGTGACGGCGGGAAAGATCGCCAGCAACGTGC AGAAGAAGCTCACCCGCGCGCAGGAGAAGGTTCTCCAGAAGCTGGGGAAGGCAGATGAGACCAAGGATGAGCAGTTTGAGCAGTGCGTCCAGAATTTCAA CAAGCAGCTGACGGAGGGCACCCGGCTGCAGAAGGATCTCCGGACCTACCTGGCCTCCGTCAAAGCCATGCACGAGGCTTCCAAGAAGCTGAATGAGTGT CTGCAGGAGGTGTATGAGCCCGATTGGCCCGGCAGGGATGAGGCAAACAAGATCGCAGAGAACAACGACCTGCTGTGGATGGATTACCACCAGAAGCTGG TGGACCAGGCGCTGCTGACCATGGACACGTACCTGGGCCAGTTCCCCGACATCAAGTCACGCATTGCCAAGCGGGGGCGCAAGCTGGTGGACTACGACAG TGCCCGGCACCACTACGAGTCCCTTCAAACTGCCAAAAAGAAGGATGAAGCCAAAATTGCCAAGGCCGAGGAGGAGCTCATCAAAGCCCAGAAGGTGTTT GAGGAGATGAATGTGGATCTGCAGGAGGAGCTGCCGTCCCTGTGGAACAGCCGCGTAGGTTTCTACGTCAACACGTTCCAGAGCATCGCGGGCCTGGAGG AAAACTTCCACAAGGAGATGAGCAAGCTCAACCAGAACCTCAATGATGTGCTGGTCGGCCTGGAGAAGCAACACGGGAGCAACACCTTCACGGTCAAGGC CCAGCCCAGTGACAACGCGCCTGCAAAAGGGAACAAGAGCCCTTCGCCTCCAGATGGCTCCCCTGCCGCCACCCCCGAGATCAGAGTCAACCACGAGCCA GAGCCGGCCGGCGGGGCCACGCCCGGGGCCACCCTCCCCAAGTCCCCATCTCAGCCCACAGAGAGTCCAGCCGGCAGCCTGCCTTCCGGGGAGCCCAGCG CTGCCGAGGGCACCTTTGCTGTGTCCTGGCCCAGCCAGACGGCCGAGCCGGGGCCTGCCCAACCAGCAGAGGCCTCGGAGGTGGCGGGTGGGACCCAACC TGCGGCTGGAGCCCAGGAGCCAGGGGAGACGGCGGCAAGTGAAGCAGCCTCCAGCTCTCTTCCTGCTGTCGTGGTGGAGACCTTCCCAGCAACTGTGAAT GGCACCGTGGAGGGCGGCAGTGGGGCCGGGCGCTTGGACCTGCCCCCAGGTTTCATGTTCAAGGTACAGGCCCAGCACGACTACACGGCCACTGACACAG ACGAGCTGCAGCTCAAGGCTGGTGATGTGGTGCTGGTGATCCCCTTCCAGAACCCTGAAGAGCAGGATGAAGGCTGGCTCATGGGCGTGAAGGAGAGCGA CTGGAACCAGCACAAGGAGCTGGAGAAGTGCCGTGGCGTCTTCCCCGAGAACTTCACTGAGAGGGTCCCATGACGGCGGGGCCCAGGCAGCCTCCGGGCG TGTGAAGAACACCTCCTCCCGAAAAATGTGTGGTTCTTTTTTTTGTTTTGTTTTCGTTTTTCATCTTTTGAAGAGCAAAGGGAAATCAAGAGGAGACCCC CAGGCAGAGGGGCGTTCTCCCAAAGATTAGGTCGTTTTCCAAAGAGCCGCGTCCCGGCAAGTCCGGCGGAATTCACCAGTGTTCCTGAAGCTGCTGTGTC CTCTAGTTGAGTTTCTGGCGCCCCTGCCTGTGCCCGCATGTGTGCCTGGCCGCAGGGCGGGGCTGGGGGCTGCCGAGCCACCATGCTTGCCTGAAGCTTC GGCCGCGCCACCCGGGCAAGGGTCCTCTTTTCCTGGCAGCTGCTGTGGGTGGGGCCCAGACACCAGCCTAGCCTGGCTCTGCCCCGCAGACGGTCTGTGT GCTGTTTGAAAATAAATCTTAGTGTTCAAAACAAAATGAAACAAAAAAAAAATGATAAAAACTCTCAG

refseq_BIN1.F7 (forward primer)
refseq_BIN1.R7 (reverse primer)
Single exon skipping

Frame 0

RAPPSSRTWRCRRPEWPFKRQLIVRRGRAGGAD RGLRPGPSPLPPSVPASLAG*LAGSLARPAHAPP PSVGSAVGCAAWSGSRSGRAAGAGGWERGAQDL PARERPLPPGEACAAMAEMGSKGVTAGKIASNV QKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFN KQLTEGTRLQKDLRTYLASVKAMHEASKKLNEC LQEVYEPDWPGRDEANKIAENNDLLWMDYHQKL VDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDS ARHHYESLQTAKKKDEAKIAKAEEELIKAQKVF EEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLE ENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKA QPSDNAPAKGNKSPSPPDGSPAATPEIRVNHEP EPAGGATPGATLPKSPSQPTESPAGSLPSGEPS AAEGTFAVSWPSQTAEPGPAQPAEASEVAGGTQP AAGAQEPGETAASEAASSSLPAVVVETFPATVN GTVEGGSGAGRLDLPPGFMFKVQAQHDYTATDT DELQLKAGDVVLVIPFQNPEEQDEGWLMGVKESD WNQHKELEKCRGVFPENFTERVP*RRGPGSLRA CEEHLLPKNVWFFFLFCFRFSSFEEQREIKRRP PGRGAFSQRLGRFPKSRVPASPAEFTSVPEAAVS SS*VSGAPACARMCAWPQGGAGGCRATMLA*SF GRATRARVLFSWQLLWVGPRHQPSLALPRRRSV CCLKINLSVQNKMKQKKNDKNSQ

Frame 1

APLPPRGPGGAGARSGPLKGSLLSGGGGRGAPT AA*GPAPPLSLPLSPRRSLASSLARSPVRRTLR LRQLAPLSGARRGAAAGLDARPGLGAGSAARKIS PRESGPCHRARPAPRWQRWAVKG*RRERSPATC RRSSPARRRRFSRSWGRQMRPRMSSLSSASRIS TSS*RRAPGCRRISGPTWPPSKPCTRLPRS*MSV CRRCMSPIGPAGMRQTRSQRTTTCCGWITTRSW WTRRC*PWTRTWASSPTSSHALPSGGASWWTTT VPGTTTSPFKLPKRRMKPKLPRPRRSSSKPRRCL RR*MWICRRSCRPCGTAA*VSTSTRSRASRAWR KTSTRR*ASSTRTSMMCWSAWRSNTGATPSRSR PSPVTTRLQKGTRALRLQMAPLPPPPRSESTTSQ SRPAGPRPGPPSPSPHLSPQRVQPAACLPGSPA LPRAPLLCPGPARRPSRGLPNQQRPRRWRVGPN LRLEPRSQGRRRQVKQPPALFLLSWWRPSQQL*M APWRAAVGPGAWTCPQVSCSRYRPSTTTRPLTQ TSCSSRLVMWCW*SPSRTLKSRMKAGSWA*RRA TGTSTRSWRSAVASSPRTSLRGSHDGGAQAASGR VKNTSSRKMCGSFFCFVFVFHLLKSKGKSRGDP QAEGRSPKD*VVFQRAASRQVRRNSPVFLKLLC PLVEFLAPLPVPACVPGRRAGLGAAEPPCLPEAS AAPPGQGSSFPGSCCGWGPDTSLAWLCPADGLC AV*K*ILVFKTK*NKKKMIKTL

Frame 2

RPSLLADLAVPAPGVAL*KAAYCPEGAGGGRRP RPEARPLPSPSLCPRVARWLARWLARPSGARSA SVSWLRCRVRGVERQPVWTRGRGWGLGARRARS PRARAAPATGRGLRRDGRDGQ*RGDGGKDRQQRA EEAHPRAGEGSPEAGEGR*DQG*AV*AVRPEFQ QAADGGHPAAEGSPDLPGLRQSHARGFQEAE*V SAGGV*ARLARQG*GKQDRREQRPAVDGLPPEAG GPGAADHGHVPGPVPRHQVTHCQAGAQAGGLRQ CPAPLRVPSNCQKEG*SQNCQGRGGAHQSPEGV *GDECGSAGGAAVPVEQPRRFLRQHVPEHRGPGG KLPQGDEQAQPEPQ*CAGRPGEATREQHLHGQG PAQ*QRACKREQEPFASRWLPCRHPRDQSQPRA RAGRRGHARGHPPQVPISAHRESSRQPAFRGAQR CRGHLCCVLAQPDGRAGACPTSRGLGGGGWDPT CGWSPGARGDGGK*SSLQLSSCCRGGDLPSNCE WHRGGRQWGRALGPAPRFHVQGTGPARLHGH*HR RAAAQGW*CGAGDPLPEP*RAG*RLAHGREGER LEPAQGAGEVPWRLPRELH*EGPMTAGPRQPPG V*RTPPPEKCVVLFFVLFSFFIF*RAKGNQEETP RQRGVLPKIRSFSKEPRPGKSGGIHQCS*SCCV L*LSFWRPCLCPHVCLAAGRGWGLPSHHACLKL RPRHPGKGPLFLAAAVGGAQTPA*PGSAPQTVCV LFENKS*CSKQNETKKK**KLS

Sequence NM_139347

CGCGCCCCTCCCTCCTCGCGGACCTGGCGGTGCCGGCGCCCGGAGTGGCCCTTTAAAAGGCAGCTTATTGTCCGGAGGGGGCGGGCGGGGGGCGCCGACC GCGGCCTGAGGCCCGGCCCCTCCCCTCTCCCTCCCTCTGTCCCCGCGTCGCTCGCTGGCTAGCTCGCTGGCTCGCTCGCCCGTCCGGCGCACGCTCCGCC TCCGTCAGTTGGCTCCGCTGTCGGGTGCGCGGCGTGGAGCGGCAGCCGGTCTGGACGCGCGGCCGGGGCTGGGGGCTGGGAGCGCGGCGCGCAAGATCTC CCCGCGCGAGAGCGGCCCCTGCCACCGGGCGAGGCCTGCGCCGCGATGGCAGAGATGGGCAGTAAAGGGGTGACGGCGGGAAAGATCGCCAGCAACGTGC AGAAGAAGCTCACCCGCGCGCAGGAGAAGGTTCTCCAGAAGCTGGGGAAGGCAGATGAGACCAAGGATGAGCAGTTTGAGCAGTGCGTCCAGAATTTCAA CAAGCAGCTGACGGAGGGCACCCGGCTGCAGAAGGATCTCCGGACCTACCTGGCCTCCGTCAAAGCCATGCACGAGGCTTCCAAGAAGCTGAATGAGTGT CTGCAGGAGGTGTATGAGCCCGATTGGCCCGGCAGGGATGAGGCAAACAAGATCGCAGAGAACAACGACCTGCTGTGGATGGATTACCACCAGAAGCTGG TGGACCAGGCGCTGCTGACCATGGACACGTACCTGGGCCAGTTCCCCGACATCAAGTCACGCATTGCCAAGCGGGGGCGCAAGCTGGTGGACTACGACAG TGCCCGGCACCACTACGAGTCCCTTCAAACTGCCAAAAAGAAGGATGAAGCCAAAATTGCCAAGGCCGAGGAGGAGCTCATCAAAGCCCAGAAGGTGTTT GAGGAGATGAATGTGGATCTGCAGGAGGAGCTGCCGTCCCTGTGGAACAGCCGCGTAGGTTTCTACGTCAACACGTTCCAGAGCATCGCGGGCCTGGAGG AAAACTTCCACAAGGAGATGAGCAAGCTCAACCAGAACCTCAATGATGTGCTGGTCGGCCTGGAGAAGCAACACGGGAGCAACACCTTCACGGTCAAGGC CCAGCCCAGTGACAACGCGCCTGCAAAAGGGAACAAGAGCCCTTCGCCTCCAGATGGCTCCCCTGCCGCCACCCCCGAGATCAGAGTCAACCACGAGCCA GAGCCGGCCGGCGGGGCCACGCCCGGGGCCACCCTCCCCAAGTCCCCATCTCAGCTCCGGAAAGGCCCACCAGTCCCTCCGCCTCCCAAACACACCCCGT CCAAGGAAGTCAAGCAGGAGCAGATCCTCAGCCTGTTTGAGGACACGTTTGTCCCTGAGATCAGCGTGACCACCCCCTCCCAGCCCACAGAGAGTCCAGC CGGCAGCCTGCCTTCCGGGGAGCCCAGCGCTGCCGAGGGCACCTTTGCTGTGTCCTGGCCCAGCCAGACGGCCGAGCCGGGGCCTGCCCAACCAGCAGAG GCCTCGGAGGTGGCGGGTGGGACCCAACCTGCGGCTGGAGCCCAGGAGCCAGGGGAGACGGCGGCAAGTGAAGCAGCCTCCAGCTCTCTTCCTGCTGTCG TGGTGGAGACCTTCCCAGCAACTGTGAATGGCACCGTGGAGGGCGGCAGTGGGGCCGGGCGCTTGGACCTGCCCCCAGGTTTCATGTTCAAGGTACAGGC CCAGCACGACTACACGGCCACTGACACAGACGAGCTGCAGCTCAAGGCTGGTGATGTGGTGCTGGTGATCCCCTTCCAGAACCCTGAAGAGCAGGATGAA GGCTGGCTCATGGGCGTGAAGGAGAGCGACTGGAACCAGCACAAGGAGCTGGAGAAGTGCCGTGGCGTCTTCCCCGAGAACTTCACTGAGAGGGTCCCAT GACGGCGGGGCCCAGGCAGCCTCCGGGCGTGTGAAGAACACCTCCTCCCGAAAAATGTGTGGTTCTTTTTTTTGTTTTGTTTTCGTTTTTCATCTTTTGA AGAGCAAAGGGAAATCAAGAGGAGACCCCCAGGCAGAGGGGCGTTCTCCCAAAGATTAGGTCGTTTTCCAAAGAGCCGCGTCCCGGCAAGTCCGGCGGAA TTCACCAGTGTTCCTGAAGCTGCTGTGTCCTCTAGTTGAGTTTCTGGCGCCCCTGCCTGTGCCCGCATGTGTGCCTGGCCGCAGGGCGGGGCTGGGGGCT GCCGAGCCACCATGCTTGCCTGAAGCTTCGGCCGCGCCACCCGGGCAAGGGTCCTCTTTTCCTGGCAGCTGCTGTGGGTGGGGCCCAGACACCAGCCTAG CCTGGCTCTGCCCCGCAGACGGTCTGTGTGCTGTTTGAAAATAAATCTTAGTGTTCAAAACAAAATGAAACAAAAAAAAAATGATAAAAACTCTCAG

refseq_BIN1.F7 (forward primer)
refseq_BIN1.R7 (reverse primer)
Single exon skipping

Frame 0

RAPPSSRTWRCRRPEWPFKRQLIVRRGRAGGAD RGLRPGPSPLPPSVPASLAG*LAGSLARPAHAPP PSVGSAVGCAAWSGSRSGRAAGAGGWERGAQDL PARERPLPPGEACAAMAEMGSKGVTAGKIASNV QKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFN KQLTEGTRLQKDLRTYLASVKAMHEASKKLNEC LQEVYEPDWPGRDEANKIAENNDLLWMDYHQKL VDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDS ARHHYESLQTAKKKDEAKIAKAEEELIKAQKVF EEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLE ENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKA QPSDNAPAKGNKSPSPPDGSPAATPEIRVNHEP EPAGGATPGATLPKSPSQLRKGPPVPPPPKHTP SKEVKQEQILSLFEDTFVPEISVTTPSQPTESPA GSLPSGEPSAAEGTFAVSWPSQTAEPGPAQPAE ASEVAGGTQPAAGAQEPGETAASEAASSSLPAV VVETFPATVNGTVEGGSGAGRLDLPPGFMFKVQA QHDYTATDTDELQLKAGDVVLVIPFQNPEEQDE GWLMGVKESDWNQHKELEKCRGVFPENFTERVP *RRGPGSLRACEEHLLPKNVWFFFLFCFRFSSFE EQREIKRRPPGRGAFSQRLGRFPKSRVPASPAE FTSVPEAAVSSS*VSGAPACARMCAWPQGGAGG CRATMLA*SFGRATRARVLFSWQLLWVGPRHQPS LALPRRRSVCCLKINLSVQNKMKQKKNDKNSQ

Frame 1

APLPPRGPGGAGARSGPLKGSLLSGGGGRGAPT AA*GPAPPLSLPLSPRRSLASSLARSPVRRTLR LRQLAPLSGARRGAAAGLDARPGLGAGSAARKIS PRESGPCHRARPAPRWQRWAVKG*RRERSPATC RRSSPARRRRFSRSWGRQMRPRMSSLSSASRIS TSS*RRAPGCRRISGPTWPPSKPCTRLPRS*MSV CRRCMSPIGPAGMRQTRSQRTTTCCGWITTRSW WTRRC*PWTRTWASSPTSSHALPSGGASWWTTT VPGTTTSPFKLPKRRMKPKLPRPRRSSSKPRRCL RR*MWICRRSCRPCGTAA*VSTSTRSRASRAWR KTSTRR*ASSTRTSMMCWSAWRSNTGATPSRSR PSPVTTRLQKGTRALRLQMAPLPPPPRSESTTSQ SRPAGPRPGPPSPSPHLSSGKAHQSLRLPNTPR PRKSSRSRSSACLRTRLSLRSA*PPPPSPQRVQ PAACLPGSPALPRAPLLCPGPARRPSRGLPNQQR PRRWRVGPNLRLEPRSQGRRRQVKQPPALFLLS WWRPSQQL*MAPWRAAVGPGAWTCPQVSCSRYR PSTTTRPLTQTSCSSRLVMWCW*SPSRTLKSRMK AGSWA*RRATGTSTRSWRSAVASSPRTSLRGSH DGGAQAASGRVKNTSSRKMCGSFFCFVFVFHLL KSKGKSRGDPQAEGRSPKD*VVFQRAASRQVRRN SPVFLKLLCPLVEFLAPLPVPACVPGRRAGLGA AEPPCLPEASAAPPGQGSSFPGSCCGWGPDTSL AWLCPADGLCAV*K*ILVFKTK*NKKKMIKTL

Frame 2

RPSLLADLAVPAPGVAL*KAAYCPEGAGGGRRP RPEARPLPSPSLCPRVARWLARWLARPSGARSA SVSWLRCRVRGVERQPVWTRGRGWGLGARRARS PRARAAPATGRGLRRDGRDGQ*RGDGGKDRQQRA EEAHPRAGEGSPEAGEGR*DQG*AV*AVRPEFQ QAADGGHPAAEGSPDLPGLRQSHARGFQEAE*V SAGGV*ARLARQG*GKQDRREQRPAVDGLPPEAG GPGAADHGHVPGPVPRHQVTHCQAGAQAGGLRQ CPAPLRVPSNCQKEG*SQNCQGRGGAHQSPEGV *GDECGSAGGAAVPVEQPRRFLRQHVPEHRGPGG KLPQGDEQAQPEPQ*CAGRPGEATREQHLHGQG PAQ*QRACKREQEPFASRWLPCRHPRDQSQPRA RAGRRGHARGHPPQVPISAPERPTSPSASQTHPV QGSQAGADPQPV*GHVCP*DQRDHPLPAHRESS RQPAFRGAQRCRGHLCCVLAQPDGRAGACPTSR GLGGGGWDPTCGWSPGARGDGGK*SSLQLSSCCR GGDLPSNCEWHRGGRQWGRALGPAPRFHVQGTG PARLHGH*HRRAAAQGW*CGAGDPLPEP*RAG* RLAHGREGERLEPAQGAGEVPWRLPRELH*EGPM TAGPRQPPGV*RTPPPEKCVVLFFVLFSFFIF* RAKGNQEETPRQRGVLPKIRSFSKEPRPGKSGG IHQCS*SCCVL*LSFWRPCLCPHVCLAAGRGWGL PSHHACLKLRPRHPGKGPLFLAAAVGGAQTPA* PGSAPQTVCVLFENKS*CSKQNETKKK**KLS

Sequence NM_004305

CGCGCCCCTCCCTCCTCGCGGACCTGGCGGTGCCGGCGCCCGGAGTGGCCCTTTAAAAGGCAGCTTATTGTCCGGAGGGGGCGGGCGGGGGGCGCCGACC GCGGCCTGAGGCCCGGCCCCTCCCCTCTCCCTCCCTCTGTCCCCGCGTCGCTCGCTGGCTAGCTCGCTGGCTCGCTCGCCCGTCCGGCGCACGCTCCGCC TCCGTCAGTTGGCTCCGCTGTCGGGTGCGCGGCGTGGAGCGGCAGCCGGTCTGGACGCGCGGCCGGGGCTGGGGGCTGGGAGCGCGGCGCGCAAGATCTC CCCGCGCGAGAGCGGCCCCTGCCACCGGGCGAGGCCTGCGCCGCGATGGCAGAGATGGGCAGTAAAGGGGTGACGGCGGGAAAGATCGCCAGCAACGTGC AGAAGAAGCTCACCCGCGCGCAGGAGAAGGTTCTCCAGAAGCTGGGGAAGGCAGATGAGACCAAGGATGAGCAGTTTGAGCAGTGCGTCCAGAATTTCAA CAAGCAGCTGACGGAGGGCACCCGGCTGCAGAAGGATCTCCGGACCTACCTGGCCTCCGTCAAAGCCATGCACGAGGCTTCCAAGAAGCTGAATGAGTGT CTGCAGGAGGTGTATGAGCCCGATTGGCCCGGCAGGGATGAGGCAAACAAGATCGCAGAGAACAACGACCTGCTGTGGATGGATTACCACCAGAAGCTGG TGGACCAGGCGCTGCTGACCATGGACACGTACCTGGGCCAGTTCCCCGACATCAAGTCACGCATTGCCAAGCGGGGGCGCAAGCTGGTGGACTACGACAG TGCCCGGCACCACTACGAGTCCCTTCAAACTGCCAAAAAGAAGGATGAAGCCAAAATTGCCAAGGCCGAGGAGGAGCTCATCAAAGCCCAGAAGGTGTTT GAGGAGATGAATGTGGATCTGCAGGAGGAGCTGCCGTCCCTGTGGAACAGCCGCGTAGGTTTCTACGTCAACACGTTCCAGAGCATCGCGGGCCTGGAGG AAAACTTCCACAAGGAGATGAGCAAGCTCAACCAGAACCTCAATGATGTGCTGGTCGGCCTGGAGAAGCAACACGGGAGCAACACCTTCACGGTCAAGGC CCAGCCCAGAAAGAAAAGTAAACTGTTTTCGCGGCTGCGCAGAAAGAAGAACAGTGACAACGCGCCTGCAAAAGGGAACAAGAGCCCTTCGCCTCCAGAT GGCTCCCCTGCCGCCACCCCCGAGATCAGAGTCAACCACGAGCCAGAGCCGGCCGGCGGGGCCACGCCCGGGGCCACCCTCCCCAAGTCCCCATCTCAGC CAGCAGAGGCCTCGGAGGTGGCGGGTGGGACCCAACCTGCGGCTGGAGCCCAGGAGCCAGGGGAGACGGCGGCAAGTGAAGCAGCCTCCAGCTCTCTTCC TGCTGTCGTGGTGGAGACCTTCCCAGCAACTGTGAATGGCACCGTGGAGGGCGGCAGTGGGGCCGGGCGCTTGGACCTGCCCCCAGGTTTCATGTTCAAG GTACAGGCCCAGCACGACTACACGGCCACTGACACAGACGAGCTGCAGCTCAAGGCTGGTGATGTGGTGCTGGTGATCCCCTTCCAGAACCCTGAAGAGC AGGATGAAGGCTGGCTCATGGGCGTGAAGGAGAGCGACTGGAACCAGCACAAGGAGCTGGAGAAGTGCCGTGGCGTCTTCCCCGAGAACTTCACTGAGAG GGTCCCATGACGGCGGGGCCCAGGCAGCCTCCGGGCGTGTGAAGAACACCTCCTCCCGAAAAATGTGTGGTTCTTTTTTTTGTTTTGTTTTCGTTTTTCA TCTTTTGAAGAGCAAAGGGAAATCAAGAGGAGACCCCCAGGCAGAGGGGCGTTCTCCCAAAGATTAGGTCGTTTTCCAAAGAGCCGCGTCCCGGCAAGTC CGGCGGAATTCACCAGTGTTCCTGAAGCTGCTGTGTCCTCTAGTTGAGTTTCTGGCGCCCCTGCCTGTGCCCGCATGTGTGCCTGGCCGCAGGGCGGGGC TGGGGGCTGCCGAGCCACCATGCTTGCCTGAAGCTTCGGCCGCGCCACCCGGGCAAGGGTCCTCTTTTCCTGGCAGCTGCTGTGGGTGGGGCCCAGACAC CAGCCTAGCCTGGCTCTGCCCCGCAGACGGTCTGTGTGCTGTTTGAAAATAAATCTTAGTGTTCAAAACAAAATGAAACAAAAAAAAAATGATAAAAACT CTCAG

refseq_BIN1.F7 (forward primer)
refseq_BIN1.R7 (reverse primer)
Single exon skipping

Frame 0

RAPPSSRTWRCRRPEWPFKRQLIVRRGRAGGAD RGLRPGPSPLPPSVPASLAG*LAGSLARPAHAPP PSVGSAVGCAAWSGSRSGRAAGAGGWERGAQDL PARERPLPPGEACAAMAEMGSKGVTAGKIASNV QKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFN KQLTEGTRLQKDLRTYLASVKAMHEASKKLNEC LQEVYEPDWPGRDEANKIAENNDLLWMDYHQKL VDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDS ARHHYESLQTAKKKDEAKIAKAEEELIKAQKVF EEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLE ENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKA QPRKKSKLFSRLRRKKNSDNAPAKGNKSPSPPD GSPAATPEIRVNHEPEPAGGATPGATLPKSPSQ PAEASEVAGGTQPAAGAQEPGETAASEAASSSLP AVVVETFPATVNGTVEGGSGAGRLDLPPGFMFK VQAQHDYTATDTDELQLKAGDVVLVIPFQNPEE QDEGWLMGVKESDWNQHKELEKCRGVFPENFTER VP*RRGPGSLRACEEHLLPKNVWFFFLFCFRFS SFEEQREIKRRPPGRGAFSQRLGRFPKSRVPAS PAEFTSVPEAAVSSS*VSGAPACARMCAWPQGGA GGCRATMLA*SFGRATRARVLFSWQLLWVGPRH QPSLALPRRRSVCCLKINLSVQNKMKQKKNDKN SQ

Frame 1

APLPPRGPGGAGARSGPLKGSLLSGGGGRGAPT AA*GPAPPLSLPLSPRRSLASSLARSPVRRTLR LRQLAPLSGARRGAAAGLDARPGLGAGSAARKIS PRESGPCHRARPAPRWQRWAVKG*RRERSPATC RRSSPARRRRFSRSWGRQMRPRMSSLSSASRIS TSS*RRAPGCRRISGPTWPPSKPCTRLPRS*MSV CRRCMSPIGPAGMRQTRSQRTTTCCGWITTRSW WTRRC*PWTRTWASSPTSSHALPSGGASWWTTT VPGTTTSPFKLPKRRMKPKLPRPRRSSSKPRRCL RR*MWICRRSCRPCGTAA*VSTSTRSRASRAWR KTSTRR*ASSTRTSMMCWSAWRSNTGATPSRSR PSPERKVNCFRGCAERRTVTTRLQKGTRALRLQM APLPPPPRSESTTSQSRPAGPRPGPPSPSPHLS QQRPRRWRVGPNLRLEPRSQGRRRQVKQPPALF LLSWWRPSQQL*MAPWRAAVGPGAWTCPQVSCSR YRPSTTTRPLTQTSCSSRLVMWCW*SPSRTLKS RMKAGSWA*RRATGTSTRSWRSAVASSPRTSLR GSHDGGAQAASGRVKNTSSRKMCGSFFCFVFVFH LLKSKGKSRGDPQAEGRSPKD*VVFQRAASRQV RRNSPVFLKLLCPLVEFLAPLPVPACVPGRRAG LGAAEPPCLPEASAAPPGQGSSFPGSCCGWGPDT SLAWLCPADGLCAV*K*ILVFKTK*NKKKMIKT L

Frame 2

RPSLLADLAVPAPGVAL*KAAYCPEGAGGGRRP RPEARPLPSPSLCPRVARWLARWLARPSGARSA SVSWLRCRVRGVERQPVWTRGRGWGLGARRARS PRARAAPATGRGLRRDGRDGQ*RGDGGKDRQQRA EEAHPRAGEGSPEAGEGR*DQG*AV*AVRPEFQ QAADGGHPAAEGSPDLPGLRQSHARGFQEAE*V SAGGV*ARLARQG*GKQDRREQRPAVDGLPPEAG GPGAADHGHVPGPVPRHQVTHCQAGAQAGGLRQ CPAPLRVPSNCQKEG*SQNCQGRGGAHQSPEGV *GDECGSAGGAAVPVEQPRRFLRQHVPEHRGPGG KLPQGDEQAQPEPQ*CAGRPGEATREQHLHGQG PAQKEK*TVFAAAQKEEQ*QRACKREQEPFASR WLPCRHPRDQSQPRARAGRRGHARGHPPQVPISA SRGLGGGGWDPTCGWSPGARGDGGK*SSLQLSS CCRGGDLPSNCEWHRGGRQWGRALGPAPRFHVQ GTGPARLHGH*HRRAAAQGW*CGAGDPLPEP*RA G*RLAHGREGERLEPAQGAGEVPWRLPRELH*E GPMTAGPRQPPGV*RTPPPEKCVVLFFVLFSFF IF*RAKGNQEETPRQRGVLPKIRSFSKEPRPGKS GGIHQCS*SCCVL*LSFWRPCLCPHVCLAAGRG WGLPSHHACLKLRPRHPGKGPLFLAAAVGGAQT PA*PGSAPQTVCVLFENKS*CSKQNETKKK**KL S

Sequence NM_139346

CGCGCCCCTCCCTCCTCGCGGACCTGGCGGTGCCGGCGCCCGGAGTGGCCCTTTAAAAGGCAGCTTATTGTCCGGAGGGGGCGGGCGGGGGGCGCCGACC GCGGCCTGAGGCCCGGCCCCTCCCCTCTCCCTCCCTCTGTCCCCGCGTCGCTCGCTGGCTAGCTCGCTGGCTCGCTCGCCCGTCCGGCGCACGCTCCGCC TCCGTCAGTTGGCTCCGCTGTCGGGTGCGCGGCGTGGAGCGGCAGCCGGTCTGGACGCGCGGCCGGGGCTGGGGGCTGGGAGCGCGGCGCGCAAGATCTC CCCGCGCGAGAGCGGCCCCTGCCACCGGGCGAGGCCTGCGCCGCGATGGCAGAGATGGGCAGTAAAGGGGTGACGGCGGGAAAGATCGCCAGCAACGTGC AGAAGAAGCTCACCCGCGCGCAGGAGAAGGTTCTCCAGAAGCTGGGGAAGGCAGATGAGACCAAGGATGAGCAGTTTGAGCAGTGCGTCCAGAATTTCAA CAAGCAGCTGACGGAGGGCACCCGGCTGCAGAAGGATCTCCGGACCTACCTGGCCTCCGTCAAAGCCATGCACGAGGCTTCCAAGAAGCTGAATGAGTGT CTGCAGGAGGTGTATGAGCCCGATTGGCCCGGCAGGGATGAGGCAAACAAGATCGCAGAGAACAACGACCTGCTGTGGATGGATTACCACCAGAAGCTGG TGGACCAGGCGCTGCTGACCATGGACACGTACCTGGGCCAGTTCCCCGACATCAAGTCACGCATTGCCAAGCGGGGGCGCAAGCTGGTGGACTACGACAG TGCCCGGCACCACTACGAGTCCCTTCAAACTGCCAAAAAGAAGGATGAAGCCAAAATTGCCAAGGCCGAGGAGGAGCTCATCAAAGCCCAGAAGGTGTTT GAGGAGATGAATGTGGATCTGCAGGAGGAGCTGCCGTCCCTGTGGAACAGCCGCGTAGGTTTCTACGTCAACACGTTCCAGAGCATCGCGGGCCTGGAGG AAAACTTCCACAAGGAGATGAGCAAGCTCAACCAGAACCTCAATGATGTGCTGGTCGGCCTGGAGAAGCAACACGGGAGCAACACCTTCACGGTCAAGGC CCAGCCCAGAAAGAAAAGTAAACTGTTTTCGCGGCTGCGCAGAAAGAAGAACAGTGACAACGCGCCTGCAAAAGGGAACAAGAGCCCTTCGCCTCCAGAT GGCTCCCCTGCCGCCACCCCCGAGATCAGAGTCAACCACGAGCCAGAGCCGGCCGGCGGGGCCACGCCCGGGGCCACCCTCCCCAAGTCCCCATCTCAGC TCCGGAAAGGCCCACCAGTCCCTCCGCCTCCCAAACACACCCCGTCCAAGGAAGTCAAGCAGGAGCAGATCCTCAGCCTGTTTGAGGACACGTTTGTCCC TGAGATCAGCGTGACCACCCCCTCCCAGCCAGCAGAGGCCTCGGAGGTGGCGGGTGGGACCCAACCTGCGGCTGGAGCCCAGGAGCCAGGGGAGACGGCG GCAAGTGAAGCAGCCTCCAGCTCTCTTCCTGCTGTCGTGGTGGAGACCTTCCCAGCAACTGTGAATGGCACCGTGGAGGGCGGCAGTGGGGCCGGGCGCT TGGACCTGCCCCCAGGTTTCATGTTCAAGGTACAGGCCCAGCACGACTACACGGCCACTGACACAGACGAGCTGCAGCTCAAGGCTGGTGATGTGGTGCT GGTGATCCCCTTCCAGAACCCTGAAGAGCAGGATGAAGGCTGGCTCATGGGCGTGAAGGAGAGCGACTGGAACCAGCACAAGGAGCTGGAGAAGTGCCGT GGCGTCTTCCCCGAGAACTTCACTGAGAGGGTCCCATGACGGCGGGGCCCAGGCAGCCTCCGGGCGTGTGAAGAACACCTCCTCCCGAAAAATGTGTGGT TCTTTTTTTTGTTTTGTTTTCGTTTTTCATCTTTTGAAGAGCAAAGGGAAATCAAGAGGAGACCCCCAGGCAGAGGGGCGTTCTCCCAAAGATTAGGTCG TTTTCCAAAGAGCCGCGTCCCGGCAAGTCCGGCGGAATTCACCAGTGTTCCTGAAGCTGCTGTGTCCTCTAGTTGAGTTTCTGGCGCCCCTGCCTGTGCC CGCATGTGTGCCTGGCCGCAGGGCGGGGCTGGGGGCTGCCGAGCCACCATGCTTGCCTGAAGCTTCGGCCGCGCCACCCGGGCAAGGGTCCTCTTTTCCT GGCAGCTGCTGTGGGTGGGGCCCAGACACCAGCCTAGCCTGGCTCTGCCCCGCAGACGGTCTGTGTGCTGTTTGAAAATAAATCTTAGTGTTCAAAACAA AATGAAACAAAAAAAAAATGATAAAAACTCTCAG

refseq_BIN1.F7 (forward primer)
refseq_BIN1.R7 (reverse primer)
Single exon skipping

Frame 0

RAPPSSRTWRCRRPEWPFKRQLIVRRGRAGGAD RGLRPGPSPLPPSVPASLAG*LAGSLARPAHAPP PSVGSAVGCAAWSGSRSGRAAGAGGWERGAQDL PARERPLPPGEACAAMAEMGSKGVTAGKIASNV QKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFN KQLTEGTRLQKDLRTYLASVKAMHEASKKLNEC LQEVYEPDWPGRDEANKIAENNDLLWMDYHQKL VDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDS ARHHYESLQTAKKKDEAKIAKAEEELIKAQKVF EEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLE ENFHKEMSKLNQNLNDVLVGLEKQHGSNTFTVKA QPRKKSKLFSRLRRKKNSDNAPAKGNKSPSPPD GSPAATPEIRVNHEPEPAGGATPGATLPKSPSQ LRKGPPVPPPPKHTPSKEVKQEQILSLFEDTFVP EISVTTPSQPAEASEVAGGTQPAAGAQEPGETA ASEAASSSLPAVVVETFPATVNGTVEGGSGAGR LDLPPGFMFKVQAQHDYTATDTDELQLKAGDVVL VIPFQNPEEQDEGWLMGVKESDWNQHKELEKCR GVFPENFTERVP*RRGPGSLRACEEHLLPKNVW FFFLFCFRFSSFEEQREIKRRPPGRGAFSQRLGR FPKSRVPASPAEFTSVPEAAVSSS*VSGAPACA RMCAWPQGGAGGCRATMLA*SFGRATRARVLFS WQLLWVGPRHQPSLALPRRRSVCCLKINLSVQNK MKQKKNDKNSQ

Frame 1

APLPPRGPGGAGARSGPLKGSLLSGGGGRGAPT AA*GPAPPLSLPLSPRRSLASSLARSPVRRTLR LRQLAPLSGARRGAAAGLDARPGLGAGSAARKIS PRESGPCHRARPAPRWQRWAVKG*RRERSPATC RRSSPARRRRFSRSWGRQMRPRMSSLSSASRIS TSS*RRAPGCRRISGPTWPPSKPCTRLPRS*MSV CRRCMSPIGPAGMRQTRSQRTTTCCGWITTRSW WTRRC*PWTRTWASSPTSSHALPSGGASWWTTT VPGTTTSPFKLPKRRMKPKLPRPRRSSSKPRRCL RR*MWICRRSCRPCGTAA*VSTSTRSRASRAWR KTSTRR*ASSTRTSMMCWSAWRSNTGATPSRSR PSPERKVNCFRGCAERRTVTTRLQKGTRALRLQM APLPPPPRSESTTSQSRPAGPRPGPPSPSPHLS SGKAHQSLRLPNTPRPRKSSRSRSSACLRTRLS LRSA*PPPPSQQRPRRWRVGPNLRLEPRSQGRRR QVKQPPALFLLSWWRPSQQL*MAPWRAAVGPGA WTCPQVSCSRYRPSTTTRPLTQTSCSSRLVMWC W*SPSRTLKSRMKAGSWA*RRATGTSTRSWRSAV ASSPRTSLRGSHDGGAQAASGRVKNTSSRKMCG SFFCFVFVFHLLKSKGKSRGDPQAEGRSPKD*V VFQRAASRQVRRNSPVFLKLLCPLVEFLAPLPVP ACVPGRRAGLGAAEPPCLPEASAAPPGQGSSFP GSCCGWGPDTSLAWLCPADGLCAV*K*ILVFKT K*NKKKMIKTL

Frame 2

RPSLLADLAVPAPGVAL*KAAYCPEGAGGGRRP RPEARPLPSPSLCPRVARWLARWLARPSGARSA SVSWLRCRVRGVERQPVWTRGRGWGLGARRARS PRARAAPATGRGLRRDGRDGQ*RGDGGKDRQQRA EEAHPRAGEGSPEAGEGR*DQG*AV*AVRPEFQ QAADGGHPAAEGSPDLPGLRQSHARGFQEAE*V SAGGV*ARLARQG*GKQDRREQRPAVDGLPPEAG GPGAADHGHVPGPVPRHQVTHCQAGAQAGGLRQ CPAPLRVPSNCQKEG*SQNCQGRGGAHQSPEGV *GDECGSAGGAAVPVEQPRRFLRQHVPEHRGPGG KLPQGDEQAQPEPQ*CAGRPGEATREQHLHGQG PAQKEK*TVFAAAQKEEQ*QRACKREQEPFASR WLPCRHPRDQSQPRARAGRRGHARGHPPQVPISA PERPTSPSASQTHPVQGSQAGADPQPV*GHVCP *DQRDHPLPASRGLGGGGWDPTCGWSPGARGDG GK*SSLQLSSCCRGGDLPSNCEWHRGGRQWGRAL GPAPRFHVQGTGPARLHGH*HRRAAAQGW*CGA GDPLPEP*RAG*RLAHGREGERLEPAQGAGEVP WRLPRELH*EGPMTAGPRQPPGV*RTPPPEKCVV LFFVLFSFFIF*RAKGNQEETPRQRGVLPKIRS FSKEPRPGKSGGIHQCS*SCCVL*LSFWRPCLC PHVCLAAGRGWGLPSHHACLKLRPRHPGKGPLFL AAAVGGAQTPA*PGSAPQTVCVLFENKS*CSKQ NETKKK**KLS