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Sequences

Sequence NM_206925

ATAAAAGCTGCCCGGGGAAGCCAGGAGAGCGAAGGGCGGACGTACTCGCCACGGCACCCAGGCTGCGCGCACGCGGTCCCGGTGTGCAGCTGGAGAGCGA GCGGCCACCGGGAGCCCCCGGCACAGCCCGCGCCCGCCCCGCAGGAGCCCGCGAAGATGCCCCGGCGCAGCCTGCACGCGGCGGCCGTGCTCCTGCTGGT GATCTTAAAGGAACAGCCTTCCAGCCCGGCCCCAGTGAACGGTTCCAAGTGGACTTATTTTGGTCCTGATGGGGAGAATAGCTGGTCCAAGAAGTACCCG TCGTGTGGGGGCCTGCTGCAGTCCCCCATAGACCTGCACAGTGACATCCTCCAGTATGACGCCAGCCTCACGCCCCTCGAGTTCCAAGGCTACAATCTGT CTGCCAACAAGCAGTTTCTCCTGACCAACAATGGCCATTCAGTGAAGCTGAACCTGCCCTCGGACATGCACATCCAGGGCCTCCAGTCTCGCTACAGTGC CACGCAGCTGCACCTGCACTGGGGGAACCCGAATGACCCGCACGGCTCTGAGCACACCGTCAGCGGACAGCACTTCGCCGCCGAGCTGCACATTGTCCAT TATAACTCAGACCTTTATCCTGACGCCAGCACTGCCAGCAACAAGTCAGAAGGCCTCGCTGTCCTGGCTGTTCTCATTGAGATGGGCTCCTTCAATCCGT CCTATGACAAGATCTTCAGTCACCTTCAACATGTAAAGTACAAAGGCCAGGAAGCATTCGTCCCGGGATTCAACATTGAAGAGCTGCTTCCGGAGAGGAC CGCTGAATATTACCGCTACCGGGGGTCCCTGACCACACCCCCTTGCAACCCCACTGTGCTCTGGACAGTTTTCCGAAACCCCGTGCAAATTTCCCAGGAG CAGCTGCTGGCTTTGGAGACAGCCCTGTACTGCACACACATGGACGACCCTTCCCCCAGAGAAATGATCAACAACTTCCGGCAGGTCCAGAAGTTCGATG AGAGGCTGGTATACACCTCCTTCTCCCAAGGCATCATCCTCTCACTGGCCCTGGCTGGCATTCTTGGCATCTGTATTGTGGTGGTGGTGTCCATTTGGCT TTTCAGAAGGAAGAGTATCAAAAAAGGTGATAACAAGGGAGTCATTTACAAGCCAGCCACCAAGATGGAGACTGAGGCCCACGCTTGAGGTCCCCGGAGC TCCCGGGCACATCCAGGAAGGACCTTGCTTTGGACCCTACACACTTCGGCTCTCTGGACACTTGCGACACCTCAAGGTGTTCTCTGTAGCTCAATCTGCA AACATGCCAGGCCTCAGGGATCCTCTGCTGGGTGCCTCCTTGCCTTGGGACCATGGCCACCCCAGAGCCATCCGATCGATGGATGGGATGCACTCTCAGA CCAAGCAGCAGGAATTCAAAGCTGCTTGCTGTAACTGTGTGAGATTGTGAAGTGGTCTGAATTCTGGAATCACAAACCAAGCCATGCTGGTGGGCCATTA ATGGTTGGAAAACACTTTCATCCGGGGCTTTGCCAGAGCGTGCTTTCAAGTGTCCTGGAAATTCTGCTGCTTCTCCAAGCTTTCAGACAAGAATGTGCAC TCTCTGCTTAGGTTTTGCTTGGGAAACTCAACTTCTTTCCTCTGGAGACGGGGCATCTCCCTCTGATTTCCTTCTGCTATGACAAAACCTTTAATCTGCA CCTTACAACTCGGGGACAAATGGGGACAGGAAGGATCAAGTTGTAGAGAGAAAAAGAAAACAAGAGATATACATTGTGATATATTAGGGACACTTTCACA GTCCTGTCCTCTGGATCACAGACACTGCACAGACCTTAGGGAATGGCAGGTTCAAGTTCCACTTCTTGGTGGGGATGAGAAGGGAGAGAGAGCTAGAGGG ACAAAGAGAATGAGAAGACATGGATGATCTGGGAGAGTCTCACTTTGGAATCAGAATTGGAATCACATTCTGTTTATCAAGCCATAATGTAAGGACAGAA TAATACAATATTAAGTCCAAATCCAACCTCCTGTCAGTGGAGCAGTTATGTTTTATACTCTACAGATTTTACAAATAATGAGGCTGTTCCTTGAAAATGT GTTGTTGCTGTGTCCTGGAGGAGACATGAGTTCCGAGATGACCCAATCTGCCTTTGAATCTGGAGGAAATAGGCAGAAACAAAATGACTGTAGAACTTAT TCTCTGTAGGCCAAATTTCATTTCAGCCACTTCTGCAGGATCCCTACTGCCAACCTGGAATGGAGACTTTTATCTACTTCTCTCTCTCTGAAGATGTCAA ATCGTGGTTTAGATCAAATATATTTCAAGCTATAAAAGCAGGAGGTTATCTGTGCAGGGGGCTGGCATCATGTATTTAGGGGCAAGTAATAATGGAATGC TACTAAGATACTCCATATTCTTCCCCGAATCACACAGACAGTTTCTGACAGGCGCAACTCCTCCATTTTCCTCCCGCAGGTGAGAACCCTGTGGAGATGA GTCAGTGCCATGACTGAGAAGGAACCGACCCCTAGTTGAGAGCACCTTGCAGTTCCCCGAGAACTTTCTGATTCACAGTCTCATTTTGACAGCATGAAAT GTCCTCTTGAAGCATAGCTTTTTAAATATCTTTTTCCTTCTACTCCTCCCTCTGACTCTAAGAATTCTCTCTTCTGGAATCGCTTGAACCCAGGAGGCGG AGGTTGCAGTAAGCCAAGGTCATGCCACTGCACTCTAGCCTGGGTGACAGAGCGAGACTCCATCTCAAAAAAAAAAAAAAAAAAATTATTCTGTACCATC ACAACTTTTCACAACGATGGCAAGCCTTATGTCTTGGGAGCCTGTTTTGCTAGGCAAAGTTACAAGTGACCTAATGGGAGCTCAAATGTGTGTGTGTCTC TCTGTGTGTTTGTGTGTGTGTGTGCACTCAAGACCTCTAACAGCCTCGAAGCCTGGGGTGGCATCCCGGCCTTGCCATTAGCATGCCTCATGCATCATCA GATGACAAGGACAACCCTCATGACGAAGCAACATGAATTAGGGGGCCTCTTGGCCTTGGTCCAAAATTGTCAATCAGAAATGAACATAAAGGACTCCAGA GCAGTGGGACTGTCTGTCAAAAGACTCTGTATATCTTTTGTGGATGAGTTTTGTGAGAGAACAGAGAGACCATTGTACCTGGCACAAGGGCTGTTCATGA AAAGGGAGACTTACTGGGAGGTGCAAGACAGTGGCATTTCTCCTCTCCTCTTGCTGCTCAGCACAGCCCTGGATTGCAGCCCCGAGGCTGAGACCAGACA AAGCCCGGGAGGCAGAAAGATGCTCCAAGAACCAACACTATCAATGTCTTTGCAAATCCTCACAGGATTCCTGTGGGTCCAGCTTTGGAACTGGGAAACC TTTCTTCGGATCCGCACTCATTCCACTGATGCCAGCTGCCCCTGAAGGATGCCAGTACTGTGGTGTGTGAGTCTCAGCAGCCGCCCACACGCTCCTAACT CTGCTGCATGGCAGATGCCTAGGTGGAAATAGCAAAAACAAGGCCCAGGCTGGGGCCAGGGCCAGAGGGGAAGGCCCTGGATTCTCACTCATGTGAGATC TTGAATCTCTTTCTTTGTTCTGTTTGTTTAGTTAGTATCATCTGGTAAAATAGTTAAAAAACAACAAAAAACTCTGTATCTGTTTCTAGCATGTGCTGCA TTGACTCTATTAATCACATTTCAAATTCACCCTACATTCCTCTCCTCTTCACTAGCCTCTCTGAAGGTGTCCTGGCCAGCCCTGGAGAAGCACTGGTGTC TGCAGCACCCCTCAGTTCCTGTGCCTCAGCCCACAGGCCACTGTGATAATGGTCTGTTTAGCACTTCTGTATTTATTGTAAGAATGATTATAATGAAGAT ACACACTGTAACTACAAGAAATTATAAATGTTTTTCACATCA

refseq_CA12.F2 (forward primer)
refseq_CA12.R2 (reverse primer)
Single exon skipping

Frame 0

IKAARGSQESEGRTYSPRHPGCAHAVPVCSWRA SGHREPPAQPAPAPQEPAKMPRRSLHAAAVLLLV ILKEQPSSPAPVNGSKWTYFGPDGENSWSKKYP SCGGLLQSPIDLHSDILQYDASLTPLEFQGYNL SANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSA TQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVH YNSDLYPDASTASNKSEGLAVLAVLIEMGSFNP SYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERT AEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQE QLLALETALYCTHMDDPSPREMINNFRQVQKFD ERLVYTSFSQGIILSLALAGILGICIVVVVSIWL FRRKSIKKGDNKGVIYKPATKMETEAHA*GPRS SRAHPGRTLLWTLHTSALWTLATPQGVLCSSIC KHARPQGSSAGCLLALGPWPPQSHPIDGWDALSD QAAGIQSCLL*LCEIVKWSEFWNHKPSHAGGPL MVGKHFHPGLCQSVLSSVLEILLLLQAFRQECA LSA*VLLGKLNFFPLETGHLPLISFCYDKTFNLH LTTRGQMGTGRIKL*REKENKRYTL*YIRDTFT VLSSGSQTLHRP*GMAGSSSTSWWG*EGRES*R DKENEKTWMIWESLTLESELESHSVYQAIM*GQN NTILSPNPTSCQWSSYVLYSTDFTNNEAVP*KC VVAVSWRRHEFRDDPICL*IWRK*AETK*L*NL FSVGQISFQPLLQDPYCQPGMETFIYFSLSEDVK SWFRSNIFQAIKAGGYLCRGLASCI*GQVIMEC Y*DTPYSSPNHTDSF*QAQLLHFPPAGENPVEM SQCHD*EGTDP*LRAPCSSPRTF*FTVSF*QHEM SS*SIAF*ISFSFYSSL*L*EFSLLESLEPRRR RLQ*AKVMPLHSSLGDRARLHLKKKKKKIILYH HNFSQRWQALCLGSLFC*AKLQVT*WELKCVCVS LCVCVCVCTQDL*QPRSLGWHPGLAISMPHASS DDKDNPHDEAT*IRGPLGLGPKLSIRNEHKGLQ SSGTVCQKTLYIFCG*VL*ENRETIVPGTRAVHE KGDLLGGARQWHFSSPLAAQHSPGLQPRG*DQT KPGRQKDAPRTNTINVFANPHRIPVGPALELGN LSSDPHSFH*CQLPLKDASTVVCESQQPPTRS*L CCMADA*VEIAKTRPRLGPGPEGKALDSHSCEI LNLFLCSVCLVSIIW*NS*KTTKNSVSVSSMCC IDSINHISNSPYIPLLFTSLSEGVLASPGEALVS AAPLSSCASAHRPL**WSV*HFCIYCKNDYNED THCNYKKL*MFFTS

Frame 1

*KLPGEARRAKGGRTRHGTQAARTRSRCAAGER AATGSPRHSPRPPRRSPRRCPGAACTRRPCSCW *S*RNSLPARPQ*TVPSGLILVLMGRIAGPRSTR RVGACCSPP*TCTVTSSSMTPASRPSSSKATIC LPTSSFS*PTMAIQ*S*TCPRTCTSRASSLATV PRSCTCTGGTRMTRTALSTPSADSTSPPSCTLSI ITQTFILTPALPATSQKASLSWLFSLRWAPSIR PMTRSSVTFNM*STKARKHSSRDSTLKSCFRRG PLNITATGGP*PHPLATPLCSGQFSETPCKFPRS SCWLWRQPCTAHTWTTLPPEK*STTSGRSRSSM RGWYTPPSPKASSSHWPWLAFLASVLWWWCPFG FSEGRVSKKVITRESFTSQPPRWRLRPTLEVPGA PGHIQEGPCFGPYTLRLSGHLRHLKVFSVAQSA NMPGLRDPLLGASLPWDHGHPRAIRSMDGMHSQ TKQQEFKAACCNCVRL*SGLNSGITNQAMLVGH* WLENTFIRGFARACFQVSWKFCCFSKLSDKNVH SLLRFCLGNSTSFLWRRGISL*FPSAMTKPLIC TLQLGDKWGQEGSSCREKKKTRDIHCDILGTLSQ SCPLDHRHCTDLREWQVQVPLLGGDEKGERARG TKRMRRHG*SGRVSLWNQNWNHILFIKP*CKDR IIQY*VQIQPPVSGAVMFYTLQILQIMRLFLENV LLLCPGGDMSSEMTQSAFESGGNRQKQNDCRTY SL*AKFHFSHFCRIPTANLEWRLLSTSLSLKMS NRGLDQIYFKL*KQEVICAGGWHHVFRGK**WNA TKILHILPRITQTVSDRRNSSIFLPQVRTLWR* VSAMTEKEPTPS*EHLAVPRELSDSQSHFDSMK CPLEA*LFKYLFPSTPPSDSKNSLFWNRLNPGGG GCSKPRSCHCTLAWVTERDSISKKKKKKLFCTI TTFHNDGKPYVLGACFARQSYK*PNGSSNVCVS LCVFVCVCALKTSNSLEAWGGIPALPLACLMHHQ MTRTTLMTKQHELGGLLALVQNCQSEMNIKDSR AVGLSVKRLCISFVDEFCERTERPLYLAQGLFM KRETYWEVQDSGISPLLLLLSTALDCSPEAETRQ SPGGRKMLQEPTLSMSLQILTGFLWVQLWNWET FLRIRTHSTDASCP*RMPVLWCVSLSSRPHAPN SAAWQMPRWK*QKQGPGWGQGQRGRPWILTHVRS *ISFFVLFV*LVSSGKIVKKQQKTLYLFLACAA LTLLITFQIHPTFLSSSLASLKVSWPALEKHWC LQHPSVPVPQPTGHCDNGLFSTSVFIVRMIIMKI HTVTTRNYKCFSH

Frame 2

KSCPGKPGERRADVLATAPRLRARGPGVQLESE RPPGAPGTARARPAGAREDAPAQPARGGRAPAG DLKGTAFQPGPSERFQVDLFWS*WGE*LVQEVP VVWGPAAVPHRPAQ*HPPV*RQPHAPRVPRLQSV CQQAVSPDQQWPFSEAEPALGHAHPGPPVSLQC HAAAPALGEPE*PARL*AHRQRTALRRRAAHCP L*LRPLS*RQHCQQQVRRPRCPGCSH*DGLLQSV L*QDLQSPSTCKVQRPGSIRPGIQH*RAASGED R*ILPLPGVPDHTPLQPHCALDSFPKPRANFPG AAAGFGDSPVLHTHGRPFPQRNDQQLPAGPEVR* EAGIHLLLPRHHPLTGPGWHSWHLYCGGGVHLA FQKEEYQKR**QGSHLQASHQDGD*GPRLRSPE LPGTSRKDLALDPTHFGSLDTCDTSRCSL*LNLQ TCQASGILCWVPPCLGTMATPEPSDRWMGCTLR PSSRNSKLLAVTV*DCEVV*ILESQTKPCWWAI NGWKTLSSGALPERAFKCPGNSAASPSFQTRMCT LCLGFAWETQLLSSGDGASPSDFLLL*QNL*SA PYNSGTNGDRKDQVVERKRKQEIYIVIY*GHFH SPVLWITDTAQTLGNGRFKFHFLVGMRRERELEG QRE*EDMDDLGESHFGIRIGITFCLSSHNVRTE *YNIKSKSNLLSVEQLCFILYRFYK**GCSLKM CCCCVLEET*VPR*PNLPLNLEEIGRNKMTVELI LCRPNFISATSAGSLLPTWNGDFYLLLSL*RCQ IVV*IKYISSYKSRRLSVQGAGIMYLGASNNGM LLRYSIFFPESHRQFLTGATPPFSSRR*EPCGDE SVP*LRRNRPLVESTLQFPENFLIHSLILTA*N VLLKHSFLNIFFLLLLPLTLRILSSGIA*TQEA EVAVSQGHATAL*PG*QSETPSQKKKKKNYSVPS QLFTTMASLMSWEPVLLGKVTSDLMGAQMCVCL SVCLCVCVHSRPLTASKPGVASRPCH*HASCII R*QGQPS*RSNMN*GASWPWSKIVNQK*T*RTPE QWDCLSKDSVYLLWMSFVREQRDHCTWHKGCS* KGRLTGRCKTVAFLLSSCCSAQPWIAAPRLRPD KAREAERCSKNQHYQCLCKSSQDSCGSSFGTGKP FFGSALIPLMPAAPEGCQYCGV*VSAAAHTLLT LLHGRCLGGNSKNKAQAGARARGEGPGFSLM*D LESLSLFCLFS*YHLVK*LKNNKKLCICF*HVLH *LY*SHFKFTLHSSPLH*PL*RCPGQPWRSTGV CSTPQFLCLSPQATVIMVCLALLYLL*E*L**R YTL*LQEIINVFHI

Sequence NM_001218

ATAAAAGCTGCCCGGGGAAGCCAGGAGAGCGAAGGGCGGACGTACTCGCCACGGCACCCAGGCTGCGCGCACGCGGTCCCGGTGTGCAGCTGGAGAGCGA GCGGCCACCGGGAGCCCCCGGCACAGCCCGCGCCCGCCCCGCAGGAGCCCGCGAAGATGCCCCGGCGCAGCCTGCACGCGGCGGCCGTGCTCCTGCTGGT GATCTTAAAGGAACAGCCTTCCAGCCCGGCCCCAGTGAACGGTTCCAAGTGGACTTATTTTGGTCCTGATGGGGAGAATAGCTGGTCCAAGAAGTACCCG TCGTGTGGGGGCCTGCTGCAGTCCCCCATAGACCTGCACAGTGACATCCTCCAGTATGACGCCAGCCTCACGCCCCTCGAGTTCCAAGGCTACAATCTGT CTGCCAACAAGCAGTTTCTCCTGACCAACAATGGCCATTCAGTGAAGCTGAACCTGCCCTCGGACATGCACATCCAGGGCCTCCAGTCTCGCTACAGTGC CACGCAGCTGCACCTGCACTGGGGGAACCCGAATGACCCGCACGGCTCTGAGCACACCGTCAGCGGACAGCACTTCGCCGCCGAGCTGCACATTGTCCAT TATAACTCAGACCTTTATCCTGACGCCAGCACTGCCAGCAACAAGTCAGAAGGCCTCGCTGTCCTGGCTGTTCTCATTGAGATGGGCTCCTTCAATCCGT CCTATGACAAGATCTTCAGTCACCTTCAACATGTAAAGTACAAAGGCCAGGAAGCATTCGTCCCGGGATTCAACATTGAAGAGCTGCTTCCGGAGAGGAC CGCTGAATATTACCGCTACCGGGGGTCCCTGACCACACCCCCTTGCAACCCCACTGTGCTCTGGACAGTTTTCCGAAACCCCGTGCAAATTTCCCAGGAG CAGCTGCTGGCTTTGGAGACAGCCCTGTACTGCACACACATGGACGACCCTTCCCCCAGAGAAATGATCAACAACTTCCGGCAGGTCCAGAAGTTCGATG AGAGGCTGGTATACACCTCCTTCTCCCAAGTGCAAGTCTGTACTGCGGCAGGACTGAGTCTGGGCATCATCCTCTCACTGGCCCTGGCTGGCATTCTTGG CATCTGTATTGTGGTGGTGGTGTCCATTTGGCTTTTCAGAAGGAAGAGTATCAAAAAAGGTGATAACAAGGGAGTCATTTACAAGCCAGCCACCAAGATG GAGACTGAGGCCCACGCTTGAGGTCCCCGGAGCTCCCGGGCACATCCAGGAAGGACCTTGCTTTGGACCCTACACACTTCGGCTCTCTGGACACTTGCGA CACCTCAAGGTGTTCTCTGTAGCTCAATCTGCAAACATGCCAGGCCTCAGGGATCCTCTGCTGGGTGCCTCCTTGCCTTGGGACCATGGCCACCCCAGAG CCATCCGATCGATGGATGGGATGCACTCTCAGACCAAGCAGCAGGAATTCAAAGCTGCTTGCTGTAACTGTGTGAGATTGTGAAGTGGTCTGAATTCTGG AATCACAAACCAAGCCATGCTGGTGGGCCATTAATGGTTGGAAAACACTTTCATCCGGGGCTTTGCCAGAGCGTGCTTTCAAGTGTCCTGGAAATTCTGC TGCTTCTCCAAGCTTTCAGACAAGAATGTGCACTCTCTGCTTAGGTTTTGCTTGGGAAACTCAACTTCTTTCCTCTGGAGACGGGGCATCTCCCTCTGAT TTCCTTCTGCTATGACAAAACCTTTAATCTGCACCTTACAACTCGGGGACAAATGGGGACAGGAAGGATCAAGTTGTAGAGAGAAAAAGAAAACAAGAGA TATACATTGTGATATATTAGGGACACTTTCACAGTCCTGTCCTCTGGATCACAGACACTGCACAGACCTTAGGGAATGGCAGGTTCAAGTTCCACTTCTT GGTGGGGATGAGAAGGGAGAGAGAGCTAGAGGGACAAAGAGAATGAGAAGACATGGATGATCTGGGAGAGTCTCACTTTGGAATCAGAATTGGAATCACA TTCTGTTTATCAAGCCATAATGTAAGGACAGAATAATACAATATTAAGTCCAAATCCAACCTCCTGTCAGTGGAGCAGTTATGTTTTATACTCTACAGAT TTTACAAATAATGAGGCTGTTCCTTGAAAATGTGTTGTTGCTGTGTCCTGGAGGAGACATGAGTTCCGAGATGACCCAATCTGCCTTTGAATCTGGAGGA AATAGGCAGAAACAAAATGACTGTAGAACTTATTCTCTGTAGGCCAAATTTCATTTCAGCCACTTCTGCAGGATCCCTACTGCCAACCTGGAATGGAGAC TTTTATCTACTTCTCTCTCTCTGAAGATGTCAAATCGTGGTTTAGATCAAATATATTTCAAGCTATAAAAGCAGGAGGTTATCTGTGCAGGGGGCTGGCA TCATGTATTTAGGGGCAAGTAATAATGGAATGCTACTAAGATACTCCATATTCTTCCCCGAATCACACAGACAGTTTCTGACAGGCGCAACTCCTCCATT TTCCTCCCGCAGGTGAGAACCCTGTGGAGATGAGTCAGTGCCATGACTGAGAAGGAACCGACCCCTAGTTGAGAGCACCTTGCAGTTCCCCGAGAACTTT CTGATTCACAGTCTCATTTTGACAGCATGAAATGTCCTCTTGAAGCATAGCTTTTTAAATATCTTTTTCCTTCTACTCCTCCCTCTGACTCTAAGAATTC TCTCTTCTGGAATCGCTTGAACCCAGGAGGCGGAGGTTGCAGTAAGCCAAGGTCATGCCACTGCACTCTAGCCTGGGTGACAGAGCGAGACTCCATCTCA AAAAAAAAAAAAAAAAAATTATTCTGTACCATCACAACTTTTCACAACGATGGCAAGCCTTATGTCTTGGGAGCCTGTTTTGCTAGGCAAAGTTACAAGT GACCTAATGGGAGCTCAAATGTGTGTGTGTCTCTCTGTGTGTTTGTGTGTGTGTGTGCACTCAAGACCTCTAACAGCCTCGAAGCCTGGGGTGGCATCCC GGCCTTGCCATTAGCATGCCTCATGCATCATCAGATGACAAGGACAACCCTCATGACGAAGCAACATGAATTAGGGGGCCTCTTGGCCTTGGTCCAAAAT TGTCAATCAGAAATGAACATAAAGGACTCCAGAGCAGTGGGACTGTCTGTCAAAAGACTCTGTATATCTTTTGTGGATGAGTTTTGTGAGAGAACAGAGA GACCATTGTACCTGGCACAAGGGCTGTTCATGAAAAGGGAGACTTACTGGGAGGTGCAAGACAGTGGCATTTCTCCTCTCCTCTTGCTGCTCAGCACAGC CCTGGATTGCAGCCCCGAGGCTGAGACCAGACAAAGCCCGGGAGGCAGAAAGATGCTCCAAGAACCAACACTATCAATGTCTTTGCAAATCCTCACAGGA TTCCTGTGGGTCCAGCTTTGGAACTGGGAAACCTTTCTTCGGATCCGCACTCATTCCACTGATGCCAGCTGCCCCTGAAGGATGCCAGTACTGTGGTGTG TGAGTCTCAGCAGCCGCCCACACGCTCCTAACTCTGCTGCATGGCAGATGCCTAGGTGGAAATAGCAAAAACAAGGCCCAGGCTGGGGCCAGGGCCAGAG GGGAAGGCCCTGGATTCTCACTCATGTGAGATCTTGAATCTCTTTCTTTGTTCTGTTTGTTTAGTTAGTATCATCTGGTAAAATAGTTAAAAAACAACAA AAAACTCTGTATCTGTTTCTAGCATGTGCTGCATTGACTCTATTAATCACATTTCAAATTCACCCTACATTCCTCTCCTCTTCACTAGCCTCTCTGAAGG TGTCCTGGCCAGCCCTGGAGAAGCACTGGTGTCTGCAGCACCCCTCAGTTCCTGTGCCTCAGCCCACAGGCCACTGTGATAATGGTCTGTTTAGCACTTC TGTATTTATTGTAAGAATGATTATAATGAAGATACACACTGTAACTACAAGAAATTATAAATGTTTTTCACATCA

refseq_CA12.F2 (forward primer)
refseq_CA12.R2 (reverse primer)
Single exon skipping

Frame 0

IKAARGSQESEGRTYSPRHPGCAHAVPVCSWRA SGHREPPAQPAPAPQEPAKMPRRSLHAAAVLLLV ILKEQPSSPAPVNGSKWTYFGPDGENSWSKKYP SCGGLLQSPIDLHSDILQYDASLTPLEFQGYNL SANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSA TQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVH YNSDLYPDASTASNKSEGLAVLAVLIEMGSFNP SYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERT AEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQE QLLALETALYCTHMDDPSPREMINNFRQVQKFD ERLVYTSFSQVQVCTAAGLSLGIILSLALAGILG ICIVVVVSIWLFRRKSIKKGDNKGVIYKPATKM ETEAHA*GPRSSRAHPGRTLLWTLHTSALWTLA TPQGVLCSSICKHARPQGSSAGCLLALGPWPPQS HPIDGWDALSDQAAGIQSCLL*LCEIVKWSEFW NHKPSHAGGPLMVGKHFHPGLCQSVLSSVLEIL LLLQAFRQECALSA*VLLGKLNFFPLETGHLPLI SFCYDKTFNLHLTTRGQMGTGRIKL*REKENKR YTL*YIRDTFTVLSSGSQTLHRP*GMAGSSSTS WWG*EGRES*RDKENEKTWMIWESLTLESELESH SVYQAIM*GQNNTILSPNPTSCQWSSYVLYSTD FTNNEAVP*KCVVAVSWRRHEFRDDPICL*IWR K*AETK*L*NLFSVGQISFQPLLQDPYCQPGMET FIYFSLSEDVKSWFRSNIFQAIKAGGYLCRGLA SCI*GQVIMECY*DTPYSSPNHTDSF*QAQLLH FPPAGENPVEMSQCHD*EGTDP*LRAPCSSPRTF *FTVSF*QHEMSS*SIAF*ISFSFYSSL*L*EF SLLESLEPRRRRLQ*AKVMPLHSSLGDRARLHL KKKKKKIILYHHNFSQRWQALCLGSLFC*AKLQV T*WELKCVCVSLCVCVCVCTQDL*QPRSLGWHP GLAISMPHASSDDKDNPHDEAT*IRGPLGLGPK LSIRNEHKGLQSSGTVCQKTLYIFCG*VL*ENRE TIVPGTRAVHEKGDLLGGARQWHFSSPLAAQHS PGLQPRG*DQTKPGRQKDAPRTNTINVFANPHR IPVGPALELGNLSSDPHSFH*CQLPLKDASTVVC ESQQPPTRS*LCCMADA*VEIAKTRPRLGPGPE GKALDSHSCEILNLFLCSVCLVSIIW*NS*KTT KNSVSVSSMCCIDSINHISNSPYIPLLFTSLSEG VLASPGEALVSAAPLSSCASAHRPL**WSV*HF CIYCKNDYNEDTHCNYKKL*MFFTS

Frame 1

*KLPGEARRAKGGRTRHGTQAARTRSRCAAGER AATGSPRHSPRPPRRSPRRCPGAACTRRPCSCW *S*RNSLPARPQ*TVPSGLILVLMGRIAGPRSTR RVGACCSPP*TCTVTSSSMTPASRPSSSKATIC LPTSSFS*PTMAIQ*S*TCPRTCTSRASSLATV PRSCTCTGGTRMTRTALSTPSADSTSPPSCTLSI ITQTFILTPALPATSQKASLSWLFSLRWAPSIR PMTRSSVTFNM*STKARKHSSRDSTLKSCFRRG PLNITATGGP*PHPLATPLCSGQFSETPCKFPRS SCWLWRQPCTAHTWTTLPPEK*STTSGRSRSSM RGWYTPPSPKCKSVLRQD*VWASSSHWPWLAFL ASVLWWWCPFGFSEGRVSKKVITRESFTSQPPRW RLRPTLEVPGAPGHIQEGPCFGPYTLRLSGHLR HLKVFSVAQSANMPGLRDPLLGASLPWDHGHPR AIRSMDGMHSQTKQQEFKAACCNCVRL*SGLNSG ITNQAMLVGH*WLENTFIRGFARACFQVSWKFC CFSKLSDKNVHSLLRFCLGNSTSFLWRRGISL* FPSAMTKPLICTLQLGDKWGQEGSSCREKKKTRD IHCDILGTLSQSCPLDHRHCTDLREWQVQVPLL GGDEKGERARGTKRMRRHG*SGRVSLWNQNWNH ILFIKP*CKDRIIQY*VQIQPPVSGAVMFYTLQI LQIMRLFLENVLLLCPGGDMSSEMTQSAFESGG NRQKQNDCRTYSL*AKFHFSHFCRIPTANLEWR LLSTSLSLKMSNRGLDQIYFKL*KQEVICAGGWH HVFRGK**WNATKILHILPRITQTVSDRRNSSI FLPQVRTLWR*VSAMTEKEPTPS*EHLAVPREL SDSQSHFDSMKCPLEA*LFKYLFPSTPPSDSKNS LFWNRLNPGGGGCSKPRSCHCTLAWVTERDSIS KKKKKKLFCTITTFHNDGKPYVLGACFARQSYK *PNGSSNVCVSLCVFVCVCALKTSNSLEAWGGIP ALPLACLMHHQMTRTTLMTKQHELGGLLALVQN CQSEMNIKDSRAVGLSVKRLCISFVDEFCERTE RPLYLAQGLFMKRETYWEVQDSGISPLLLLLSTA LDCSPEAETRQSPGGRKMLQEPTLSMSLQILTG FLWVQLWNWETFLRIRTHSTDASCP*RMPVLWC VSLSSRPHAPNSAAWQMPRWK*QKQGPGWGQGQR GRPWILTHVRS*ISFFVLFV*LVSSGKIVKKQQ KTLYLFLACAALTLLITFQIHPTFLSSSLASLK VSWPALEKHWCLQHPSVPVPQPTGHCDNGLFSTS VFIVRMIIMKIHTVTTRNYKCFSH

Frame 2

KSCPGKPGERRADVLATAPRLRARGPGVQLESE RPPGAPGTARARPAGAREDAPAQPARGGRAPAG DLKGTAFQPGPSERFQVDLFWS*WGE*LVQEVP VVWGPAAVPHRPAQ*HPPV*RQPHAPRVPRLQSV CQQAVSPDQQWPFSEAEPALGHAHPGPPVSLQC HAAAPALGEPE*PARL*AHRQRTALRRRAAHCP L*LRPLS*RQHCQQQVRRPRCPGCSH*DGLLQSV L*QDLQSPSTCKVQRPGSIRPGIQH*RAASGED R*ILPLPGVPDHTPLQPHCALDSFPKPRANFPG AAAGFGDSPVLHTHGRPFPQRNDQQLPAGPEVR* EAGIHLLLPSASLYCGRTESGHHPLTGPGWHSW HLYCGGGVHLAFQKEEYQKR**QGSHLQASHQD GD*GPRLRSPELPGTSRKDLALDPTHFGSLDTCD TSRCSL*LNLQTCQASGILCWVPPCLGTMATPE PSDRWMGCTLRPSSRNSKLLAVTV*DCEVV*IL ESQTKPCWWAINGWKTLSSGALPERAFKCPGNSA ASPSFQTRMCTLCLGFAWETQLLSSGDGASPSD FLLL*QNL*SAPYNSGTNGDRKDQVVERKRKQE IYIVIY*GHFHSPVLWITDTAQTLGNGRFKFHFL VGMRRERELEGQRE*EDMDDLGESHFGIRIGIT FCLSSHNVRTE*YNIKSKSNLLSVEQLCFILYR FYK**GCSLKMCCCCVLEET*VPR*PNLPLNLEE IGRNKMTVELILCRPNFISATSAGSLLPTWNGD FYLLLSL*RCQIVV*IKYISSYKSRRLSVQGAG IMYLGASNNGMLLRYSIFFPESHRQFLTGATPPF SSRR*EPCGDESVP*LRRNRPLVESTLQFPENF LIHSLILTA*NVLLKHSFLNIFFLLLLPLTLRI LSSGIA*TQEAEVAVSQGHATAL*PG*QSETPSQ KKKKKNYSVPSQLFTTMASLMSWEPVLLGKVTS DLMGAQMCVCLSVCLCVCVHSRPLTASKPGVAS RPCH*HASCIIR*QGQPS*RSNMN*GASWPWSKI VNQK*T*RTPEQWDCLSKDSVYLLWMSFVREQR DHCTWHKGCS*KGRLTGRCKTVAFLLSSCCSAQ PWIAAPRLRPDKAREAERCSKNQHYQCLCKSSQD SCGSSFGTGKPFFGSALIPLMPAAPEGCQYCGV *VSAAAHTLLTLLHGRCLGGNSKNKAQAGARAR GEGPGFSLM*DLESLSLFCLFS*YHLVK*LKNNK KLCICF*HVLH*LY*SHFKFTLHSSPLH*PL*R CPGQPWRSTGVCSTPQFLCLSPQATVIMVCLAL LYLL*E*L**RYTL*LQEIINVFHI