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Sequences

Sequence NM_033527

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGTGTTTTAACTCAAATGGGT GATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGATAAAACGCTTAA AAAATTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGGAGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCCCCGTATAGAAG AGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATCACAGAAAAGAT GAAAAGAGAAAAGAAAAATGTAGGCATCATAGCCATTCAGCAGAAGGGGGGAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCGGAAACGACATC GAGAAGAACAGGATAAAGCTCGCCGGGAATGGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGGGACCGCTTGGAGCAGTT AGAAAGGAAGCGGGAGCGGGAGCGCAAGATGCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCGGGCGGAGGAGCGGCGCAAGGAG CGGGAGGCCCGCAGGGAAGTGTCTGCACATCACCGAACGATGAGAGAGGACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAGCCCGCCTCGGC CGCCGCGGGAGCGGTTCGAGTTGGGAGACGGCCGGAAGCCAGTAAAAGAAGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACAGGACATCAGCGA CAGCGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGAATCAGGCTCAGGTTCTGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGG AGCACCAGTGAAGAATCAGAGGAGGAGGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGCCGAAG AAGTAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTCCGGGGAGAG TGAAGAAGCAGAGGAAGAAGTGGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGAGGAGGGCACCTATGGAGTGGTCTACAGAGCAAA AGACAAGAAAACAGATGAAATTGTGGCTCTAAAGCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCCCTGAGGGAGATCAACACCATC CTCAAGGCCCAGCATCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATCGTGATGAACTACGTGGAGCACGACC TCAAGAGCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAGGGGAGGTGAAGACCCTGATGATCCAGCTGCTGCGGGGGGTGAAACACCTGCACGACAA CTGGATCCTGCACCGTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTTTGGGCTGGCGCGGGAGTACGGATCC CCTCTGAAGGCCTACACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTGCTGCTTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGT CAGTGGGCTGCATCTTCGGGGAGCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCAACAAAGTGTTCAAGGAGCTGGGGAC CCCCAGTGAGAAAATCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAAAAGATGACCTTCAGCGAGCACCCCTACAACAACCTCCGCAAGCGCTTCGGG GCTCTGCTCTCAGACCAGGGCTTCGACCTCATGAACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATT TCCGCGAGACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGAAGCGGGGCACCAGCCCGAGGCCCCC TGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCTGAAGGAGACGGGCTTCCACCTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCC GGCTTCAGCCTCAAGTTCTGAAGGTCAGAGTGGACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGCFNSNG **KGLLESENFR*NSSGKETKEGTRGESRDKTLK KF**PGFQAGFP*GGGAERSLHGDHNKELPV*K RRLNGRQRRRR*FFGHQTTPANVSERKSSSQKR *KEKRKM*AS*PFSRRGEAC*SERKRARTSETTS RRTG*SSPGMGKTEEKGNGKGAFQERKGPLGAV RKEAGAGAQDAGAAEGAAGAEGARAAGGGAAQG AGGPQGSVCTSPNDERGLQRQSESQPLESQPASA AAGAVRVGRRPEASKRRENGRKGPAVRLTGHQR QREEDQLGRVLVSGIRLRF*GRRGGGGRGGGGR EHQ*RIRGGGGGRGRGGGGDRQQL*GGIRAVCRR SK*GRNE*R*RTRK*KPPLGCSRVTVRPRFRGE *RSRGRSGAAGASRSSSA*TGSRRAPMEWSTEQ KTRKQMKLWL*SG*RWRRRRRASRSRP*GRSTPS SRPSIPTLSPLERLWWAATWTRSTS**TTWSTT SRA*WRP*NSPSCQGR*RP**SSCCGG*NTCTT TGSCTVTSRRPTCC*ATPASSRWVILGWRGSTDP L*RPTPRSW*PSGTAPQSCCLVPRNTPRPWTCG QWAASSGSC*LRSLCSPGIRKSIRSTKCSRSWG PPVRKSGPATVSSQ*SKR*PSASTPTTTSASASG LCSQTRASTS*TSS*PTSPGGGSALRTASSMSI SARPPSPSTPPCSPRGPPRASSSV*SGAPARGP LREAWATASWVTTT*RRRASTLPPRTRGPLPRAP ASASSSEGQSGPRHG

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*GVLTQMG DEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRL KNSDDRDSKRDSLEEGELRDHCMEITIRNSPYRR EDSMEDRGEEDDSLAIKPPQQMSRKEKVHHRKD EKRKEKCRHHSHSAEGGKHARVKEREHERRKRH REEQDKARREWERQKRREMAREHSRRERDRLEQL ERKRERERKMREQQKEQREQKERERRAEERRKE REARREVSAHHRTMREDYSDKVKASHWSRSPPR PPRERFELGDGRKPVKEEKMEERDLLSDLQDISD SERKTSSAESSSAESGSGSEEEEEEEEEEEEEG STSEESEEEEEEEEEEEEETGSNSEEASEQSAE EVSEEEMSEDEERENENHLLVVPESRFDRDSGES EEAEEEVGLPERRGVPVPEQDRGGHLWSGLQSK RQENR*NCGSKAAEDGEGEGGLPDHVPEGDQHH PQGPASQHCHR*RDCGGQQHGQDLHRDELRGARP QEPDGDHETALPARGGEDPDDPAAAGGETPARQ LDPAP*PQDVQPAAEPRRHPQGG*FWAGAGVRI PSEGLHPGRGDPVVPRPRAAAWCQGILHGRGHVV SGLHLRGAADSEASVPREFGNRSDQQSVQGAGD PQ*ENLARLQ*APSSQKDDLQRAPLQQPPQALR GSALRPGLRPHEQVPDLLPREEDQR*GRPQA*VF PRDPPPHRPLHVPHVARQERAAACEAGHQPEAP *GRPGLQPAG*RRPEGDGLPPYHHEPGGLCRGP RLQPQVLKVRVDPVMG

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRVF*LKWV MKRTLGK*KL*MKFFRKRNEGRNKRRKQR*NA* KILMTGIPSGIPLRRGS*EITAWRSQ*GTPRIE EKTQWKTEEKKMILWPSNHPSKCLGKKKFITEKM KREKKNVGIIAIQQKGGSMLE*KKESTNVGNDI EKNRIKLAGNGKDRREGKWQGSIPGEKGTAWSS *KGSGSGSARCGSSRRSSGSRRSASGGRRSGARS GRPAGKCLHITER*ERTTATK*KPATGVAARLG RRGSGSSWETAGSQ*KKRKWKKGTCCPTYRTSA TARGRPARPSPRQRNQAQVLRKKRRRRKRRRRKG APVKNQRRRRRKRKRRRRRPAATLRRHQSSLPK K*VRKK*VKMKNEKMKTTSWLFQSHGSTEIPGR VKKQRKKWGCRSVEEFQCLNRIEEGTYGVVYRAK DKKTDEIVALKRLKMEKEKEGFPITSLREINTI LKAQHPNIVTVREIVVGSNMDKIYIVMNYVEHD LKSLMETMKQPFLPGEVKTLMIQLLRGVKHLHDN WILHRDLKTSNLLLSHAGILKVGDFGLAREYGS PLKAYTPVVVTQWYRAPELLLGAKEYSTAVDMW SVGCIFGELLTQKPLFPGNSEIDQINKVFKELGT PSEKIWPGYSELPVVKKMTFSEHPYNNLRKRFG ALLSDQGFDLMNKFLTYFPGRRISAEDGLKHEY FRETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPP EGGLGYSQLGDDDLKETGFHLTTTNQGASAAGP GFSLKF*RSEWTPSW

Sequence NM_033532

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGTGTTTTAACTCAAATGGGT GATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGATAAAACGCTTAA AAAATTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGGAGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCCCCGTATAGAAG AGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATCACAGAAAAGAT GAAAAGAGAAAAGAAAAATGTAGGCATCATAGCCATTCAGCAGAAGGGGGGAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCGGAAACGACATC GAGAAGAACAGGATAAAGCTCGCCGGGAATGGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGGGACCGCTTGGAGCAGTT AGAAAGGAAGCGGGAGCGGGAGCGCAAGATGCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCGGGCGGAGGAGCGGCGCAAGGAG CGGGAGGCCCGCAGGGAAGTGTCTGCACATCACCGAACGATGAGAGAGGACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAGCCCGCCTCGGC CGCCGCGGGAGCGGTTCGAGTTGGGAGACGGCCGGAAGCCAGGTGAGGCCAGGCCGGCGCCTGCGCAGAAGCCAGCACAGTTAAAAGAAGAGAAAATGGA AGAAAGGGACCTGCTGTCCGACTTACAGGACATCAGCGACAGCGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGAATCAGGCTCAGGTTCTGAG GAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGGAGCACCAGTGAAGAATCAGAGGAGGAGGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGACCG GCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGCCGAAGAAGTAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAAATGAAAACCACCTCTTGGT TGTTCCAGAGTCACGGTTCGACCGAGATTCCGGGGAGAGTGAAGAAGCAGAGGAAGAAGTGGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAG GATCGAGGAGGGCACCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAAAGCGGCTGAAGATGGAGAAGGAGAAGGAG GGCTTCCCGATCACGTCCCTGAGGGAGATCAACACCATCCTCAAGGCCCAGCATCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGG ACAAGATCTACATCGTGATGAACTACGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAGGGGAGGTGAAGACCCTGAT GATCCAGCTGCTGCGGGGGGTGAAACACCTGCACGACAACTGGATCCTGCACCGTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTC AAGGTGGGTGATTTTGGGCTGGCGCGGGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTGCTGC TTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGTCAGTGGGCTGCATCTTCGGGGAGCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGA AATCGATCAGATCAACAAAGTGTTCAAGGAGCTGGGGACCCCCAGTGAGAAAATCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAAAAGATGACCTTC AGCGAGCACCCCTACAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCATGAACAAGTTCCTGACCTACTTCCCCGGGA GGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATTTCCGCGAGACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGA GCAGCAGCGTGTGAAGCGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCTGAAGGAGACGGGCTTCCAC CTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTCAAGTTCTGAAGGTCAGAGTGGACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGCFNSNG **KGLLESENFR*NSSGKETKEGTRGESRDKTLK KF**PGFQAGFP*GGGAERSLHGDHNKELPV*K RRLNGRQRRRR*FFGHQTTPANVSERKSSSQKR *KEKRKM*AS*PFSRRGEAC*SERKRARTSETTS RRTG*SSPGMGKTEEKGNGKGAFQERKGPLGAV RKEAGAGAQDAGAAEGAAGAEGARAAGGGAAQG AGGPQGSVCTSPNDERGLQRQSESQPLESQPASA AAGAVRVGRRPEAR*GQAGACAEASTVKRRENG RKGPAVRLTGHQRQREEDQLGRVLVSGIRLRF* GRRGGGGRGGGGREHQ*RIRGGGGGRGRGGGGDR QQL*GGIRAVCRRSK*GRNE*R*RTRK*KPPLG CSRVTVRPRFRGE*RSRGRSGAAGASRSSSA*T GSRRAPMEWSTEQKTRKQMKLWL*SG*RWRRRRR ASRSRP*GRSTPSSRPSIPTLSPLERLWWAATW TRSTS**TTWSTTSRA*WRP*NSPSCQGR*RP* *SSCCGG*NTCTTTGSCTVTSRRPTCC*ATPASS RWVILGWRGSTDPL*RPTPRSW*PSGTAPQSCC LVPRNTPRPWTCGQWAASSGSC*LRSLCSPGIR KSIRSTKCSRSWGPPVRKSGPATVSSQ*SKR*PS ASTPTTTSASASGLCSQTRASTS*TSS*PTSPG GGSALRTASSMSISARPPSPSTPPCSPRGPPRA SSSV*SGAPARGPLREAWATASWVTTT*RRRAST LPPRTRGPLPRAPASASSSEGQSGPRHG

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*GVLTQMG DEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRL KNSDDRDSKRDSLEEGELRDHCMEITIRNSPYRR EDSMEDRGEEDDSLAIKPPQQMSRKEKVHHRKD EKRKEKCRHHSHSAEGGKHARVKEREHERRKRH REEQDKARREWERQKRREMAREHSRRERDRLEQL ERKRERERKMREQQKEQREQKERERRAEERRKE REARREVSAHHRTMREDYSDKVKASHWSRSPPR PPRERFELGDGRKPGEARPAPAQKPAQLKEEKME ERDLLSDLQDISDSERKTSSAESSSAESGSGSE EEEEEEEEEEEEGSTSEESEEEEEEEEEEEEET GSNSEEASEQSAEEVSEEEMSEDEERENENHLLV VPESRFDRDSGESEEAEEEVGLPERRGVPVPEQ DRGGHLWSGLQSKRQENR*NCGSKAAEDGEGEG GLPDHVPEGDQHHPQGPASQHCHR*RDCGGQQHG QDLHRDELRGARPQEPDGDHETALPARGGEDPD DPAAAGGETPARQLDPAP*PQDVQPAAEPRRHP QGG*FWAGAGVRIPSEGLHPGRGDPVVPRPRAAA WCQGILHGRGHVVSGLHLRGAADSEASVPREFG NRSDQQSVQGAGDPQ*ENLARLQ*APSSQKDDL QRAPLQQPPQALRGSALRPGLRPHEQVPDLLPRE EDQR*GRPQA*VFPRDPPPHRPLHVPHVARQER AAACEAGHQPEAP*GRPGLQPAG*RRPEGDGLP PYHHEPGGLCRGPRLQPQVLKVRVDPVMG

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRVF*LKWV MKRTLGK*KL*MKFFRKRNEGRNKRRKQR*NA* KILMTGIPSGIPLRRGS*EITAWRSQ*GTPRIE EKTQWKTEEKKMILWPSNHPSKCLGKKKFITEKM KREKKNVGIIAIQQKGGSMLE*KKESTNVGNDI EKNRIKLAGNGKDRREGKWQGSIPGEKGTAWSS *KGSGSGSARCGSSRRSSGSRRSASGGRRSGARS GRPAGKCLHITER*ERTTATK*KPATGVAARLG RRGSGSSWETAGSQVRPGRRLRRSQHS*KKRKW KKGTCCPTYRTSATARGRPARPSPRQRNQAQVLR KKRRRRKRRRRKGAPVKNQRRRRRKRKRRRRRP AATLRRHQSSLPKK*VRKK*VKMKNEKMKTTSW LFQSHGSTEIPGRVKKQRKKWGCRSVEEFQCLNR IEEGTYGVVYRAKDKKTDEIVALKRLKMEKEKE GFPITSLREINTILKAQHPNIVTVREIVVGSNM DKIYIVMNYVEHDLKSLMETMKQPFLPGEVKTLM IQLLRGVKHLHDNWILHRDLKTSNLLLSHAGIL KVGDFGLAREYGSPLKAYTPVVVTQWYRAPELL LGAKEYSTAVDMWSVGCIFGELLTQKPLFPGNSE IDQINKVFKELGTPSEKIWPGYSELPVVKKMTF SEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPG RRISAEDGLKHEYFRETPLPIDPSMFPTWPAKSE QQRVKRGTSPRPPEGGLGYSQLGDDDLKETGFH LTTTNQGASAAGPGFSLKF*RSEWTPSW

Sequence NM_024011

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGTGTTTTAACTCAAATGGGT GATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGATAAAACGCTTAA AAAATTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGGAGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCCCCGTATAGAAG AGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATCACAGAAAAGAT GAAAAGAGAAAAGAAAAATGTAGGCATCATAGCCATTCAGCAGAAGGGGGGAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCGGAAACGACATC GAGAAGAACAGGATAAAGCTCGCCGGGAATGGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGGGACCGCTTGGAGCAGTT AGAAAGGAAGCGGGAGCGGGAGCGCAAGATGCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCGGGCGGAGGAGCGGCGCAAGGAG CGGGAGGCCCGCAGGGAAGTGTCTGCACATCACCGAACGATGAGAGAGGACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAGCCCGCCTCGGC CGCCGCGGGAGCGGTTCGAGTTGGGAGACGGCCGGAAGCCAGTAAAAGAAGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACAGGACATCAGCGA CAGCGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGAATCAGGCTCAGGTTCTGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGG AGCACCAGTGAAGAATCAGAGGAGGAGGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGCCGAAG AAGTAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTCCGGGGAGAG TGAAGAAGCAGAGGAAGAAGTGGGTGAGGGAACGCCGCAGAGCAGCGCCCTGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGTTGCCCATCGAG CTCAAGCAGGAGCTGCCCAAGTACCTGCCGGCCCTGCAGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGAGGAGGGCACCTATGGAG TGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAAAGCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCCCTGAG GGAGATCAACACCATCCTCAAGGCCCAGCATCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATCGTGATGAAC TACGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAGGGGAGGTGAAGACCCTGATGATCCAGCTGCTGCGGGGGGTGA AACACCTGCACGACAACTGGATCCTGCACCGTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTTTGGGCTGGC GCGGGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTGCTGCTTGGTGCCAAGGAATACTCCACG GCCGTGGACATGTGGTCAGTGGGCTGCATCTTCGGGGAGCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCAACAAAGTGT TCAAGGAGCTGGGGACCCCCAGTGAGAAAATCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAAAAGATGACCTTCAGCGAGCACCCCTACAACAACCT CCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCATGAACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGC CTCAAGCATGAGTATTTCCGCGAGACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGAAGCGGGGCA CCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCTGAAGGAGACGGGCTTCCACCTTACCACCACGAACCAGGGGGC CTCTGCCGCGGGCCCCGGCTTCAGCCTCAAGTTCTGAAGGTCAGAGTGGACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGCFNSNG **KGLLESENFR*NSSGKETKEGTRGESRDKTLK KF**PGFQAGFP*GGGAERSLHGDHNKELPV*K RRLNGRQRRRR*FFGHQTTPANVSERKSSSQKR *KEKRKM*AS*PFSRRGEAC*SERKRARTSETTS RRTG*SSPGMGKTEEKGNGKGAFQERKGPLGAV RKEAGAGAQDAGAAEGAAGAEGARAAGGGAAQG AGGPQGSVCTSPNDERGLQRQSESQPLESQPASA AAGAVRVGRRPEASKRRENGRKGPAVRLTGHQR QREEDQLGRVLVSGIRLRF*GRRGGGGRGGGGR EHQ*RIRGGGGGRGRGGGGDRQQL*GGIRAVCRR SK*GRNE*R*RTRK*KPPLGCSRVTVRPRFRGE *RSRGRSG*GNAAEQRPDRGRLCARLPCPVAHR AQAGAAQVPAGPAGLPERRGVPVPEQDRGGHLWS GLQSKRQENR*NCGSKAAEDGEGEGGLPDHVPE GDQHHPQGPASQHCHR*RDCGGQQHGQDLHRDE LRGARPQEPDGDHETALPARGGEDPDDPAAAGGE TPARQLDPAP*PQDVQPAAEPRRHPQGG*FWAG AGVRIPSEGLHPGRGDPVVPRPRAAAWCQGILH GRGHVVSGLHLRGAADSEASVPREFGNRSDQQSV QGAGDPQ*ENLARLQ*APSSQKDDLQRAPLQQP PQALRGSALRPGLRPHEQVPDLLPREEDQR*GR PQA*VFPRDPPPHRPLHVPHVARQERAAACEAGH QPEAP*GRPGLQPAG*RRPEGDGLPPYHHEPGG LCRGPRLQPQVLKVRVDPVMG

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*GVLTQMG DEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRL KNSDDRDSKRDSLEEGELRDHCMEITIRNSPYRR EDSMEDRGEEDDSLAIKPPQQMSRKEKVHHRKD EKRKEKCRHHSHSAEGGKHARVKEREHERRKRH REEQDKARREWERQKRREMAREHSRRERDRLEQL ERKRERERKMREQQKEQREQKERERRAEERRKE REARREVSAHHRTMREDYSDKVKASHWSRSPPR PPRERFELGDGRKPVKEEKMEERDLLSDLQDISD SERKTSSAESSSAESGSGSEEEEEEEEEEEEEG STSEESEEEEEEEEEEEEETGSNSEEASEQSAE EVSEEEMSEDEERENENHLLVVPESRFDRDSGES EEAEEEVGEGTPQSSALTEGDYVPDSPALLPIE LKQELPKYLPALQGCRSVEEFQCLNRIEEGTYG VVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLR EINTILKAQHPNIVTVREIVVGSNMDKIYIVMN YVEHDLKSLMETMKQPFLPGEVKTLMIQLLRGV KHLHDNWILHRDLKTSNLLLSHAGILKVGDFGLA REYGSPLKAYTPVVVTQWYRAPELLLGAKEYST AVDMWSVGCIFGELLTQKPLFPGNSEIDQINKV FKELGTPSEKIWPGYSELPVVKKMTFSEHPYNNL RKRFGALLSDQGFDLMNKFLTYFPGRRISAEDG LKHEYFRETPLPIDPSMFPTWPAKSEQQRVKRG TSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGA SAAGPGFSLKF*RSEWTPSW

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRVF*LKWV MKRTLGK*KL*MKFFRKRNEGRNKRRKQR*NA* KILMTGIPSGIPLRRGS*EITAWRSQ*GTPRIE EKTQWKTEEKKMILWPSNHPSKCLGKKKFITEKM KREKKNVGIIAIQQKGGSMLE*KKESTNVGNDI EKNRIKLAGNGKDRREGKWQGSIPGEKGTAWSS *KGSGSGSARCGSSRRSSGSRRSASGGRRSGARS GRPAGKCLHITER*ERTTATK*KPATGVAARLG RRGSGSSWETAGSQ*KKRKWKKGTCCPTYRTSA TARGRPARPSPRQRNQAQVLRKKRRRRKRRRRKG APVKNQRRRRRKRKRRRRRPAATLRRHQSSLPK K*VRKK*VKMKNEKMKTTSWLFQSHGSTEIPGR VKKQRKKWVRERRRAAP*QRATMCPTPLPCCPSS SSRSCPSTCRPCRAAGASRSSSA*TGSRRAPME WSTEQKTRKQMKLWL*SG*RWRRRRRASRSRP* GRSTPSSRPSIPTLSPLERLWWAATWTRSTS**T TWSTTSRA*WRP*NSPSCQGR*RP**SSCCGG* NTCTTTGSCTVTSRRPTCC*ATPASSRWVILGW RGSTDPL*RPTPRSW*PSGTAPQSCCLVPRNTPR PWTCGQWAASSGSC*LRSLCSPGIRKSIRSTKC SRSWGPPVRKSGPATVSSQ*SKR*PSASTPTTT SASASGLCSQTRASTS*TSS*PTSPGGGSALRTA SSMSISARPPSPSTPPCSPRGPPRASSSV*SGA PARGPLREAWATASWVTTT*RRRASTLPPRTRG PLPRAPASASSSEGQSGPRHG

Sequence NM_033529

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGTGTTTTAACTCAAATGGGT GATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGATAAAACGCTTAA AAAATTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGGAGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCCCCGTATAGAAG AGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATCACAGAAAAGAT GAAAAGAGAAAAGAAAAATGGAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCGGAAACGACATCGAGAAGAACAGGATAAAGCTCGCCGGGAAT GGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGGGACCGCTTGGAGCAGTTAGAAAGGAAGCGGGAGCGGGAGCGCAAGAT GCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCGGGCGGAGGAGCGGCGCAAGGAGCGGGAGGCCCGCAGGGAAGTGTCTGCACAT CACCGAACGATGAGAGAGGACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAGCCCGCCTCGGCCGCCGCGGGAGCGGTTCGAGTTGGGAGACG GCCGGAAGCCAGTAAAAGAAGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACAGGACATCAGCGACAGCGAGAGGAAGACCAGCTCGGCCGAGTC CTCGTCAGCGGAATCAGGCTCAGGTTCTGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGGAGCACCAGTGAAGAATCAGAGGAGGAGGAG GAGGAAGAGGAAGAGGAGGAGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGCCGAAGAAGTAAGTGAGGAAGAAATGAGTGAAGATG AAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTCCGGGGAGAGTGAAGAAGCAGAGGAAGAAGTGGGTGAGGG AACGCCGCAGAGCAGCGCCCTGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGTTGCCCATCGAGCTCAAGCAGGAGCTGCCCAAGTACCTGCCG GCCCTGCAGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGAGGAGGGCACCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAG ATGAAATTGTGGCTCTAAAGCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCCCTGAGGGAGATCAACACCATCCTCAAGGCCCAGCA TCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATCGTGATGAACTACGTGGAGCACGACCTCAAGAGCCTGATG GAGACCATGAAACAGCCCTTCCTGCCAGGGGAGGTGAAGACCCTGATGATCCAGCTGCTGCGGGGGGTGAAACACCTGCACGACAACTGGATCCTGCACC GTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTTTGGGCTGGCGCGGGAGTACGGATCCCCTCTGAAGGCCTA CACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTGCTGCTTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGTCAGTGGGCTGCATC TTCGGGGAGCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCAACAAAGTGTTCAAGGAGCTGGGGACCCCCAGTGAGAAAA TCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAAAAGATGACCTTCAGCGAGCACCCCTACAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGA CCAGGGCTTCGACCTCATGAACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATTTCCGCGAGACCCCC CTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGAAGCGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGG GCTACAGCCAGCTGGGTGACGACGACCTGAAGGAGACGGGCTTCCACCTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTCAA GTTCTGAAGGTCAGAGTGGACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGCFNSNG **KGLLESENFR*NSSGKETKEGTRGESRDKTLK KF**PGFQAGFP*GGGAERSLHGDHNKELPV*K RRLNGRQRRRR*FFGHQTTPANVSERKSSSQKR *KEKRKMEAC*SERKRARTSETTSRRTG*SSPGM GKTEEKGNGKGAFQERKGPLGAVRKEAGAGAQD AGAAEGAAGAEGARAAGGGAAQGAGGPQGSVCT SPNDERGLQRQSESQPLESQPASAAAGAVRVGRR PEASKRRENGRKGPAVRLTGHQRQREEDQLGRV LVSGIRLRF*GRRGGGGRGGGGREHQ*RIRGGG GGRGRGGGGDRQQL*GGIRAVCRRSK*GRNE*R* RTRK*KPPLGCSRVTVRPRFRGE*RSRGRSG*G NAAEQRPDRGRLCARLPCPVAHRAQAGAAQVPA GPAGLPERRGVPVPEQDRGGHLWSGLQSKRQENR *NCGSKAAEDGEGEGGLPDHVPEGDQHHPQGPA SQHCHR*RDCGGQQHGQDLHRDELRGARPQEPD GDHETALPARGGEDPDDPAAAGGETPARQLDPAP *PQDVQPAAEPRRHPQGG*FWAGAGVRIPSEGL HPGRGDPVVPRPRAAAWCQGILHGRGHVVSGLH LRGAADSEASVPREFGNRSDQQSVQGAGDPQ*EN LARLQ*APSSQKDDLQRAPLQQPPQALRGSALR PGLRPHEQVPDLLPREEDQR*GRPQA*VFPRDP PPHRPLHVPHVARQERAAACEAGHQPEAP*GRPG LQPAG*RRPEGDGLPPYHHEPGGLCRGPRLQPQ VLKVRVDPVMG

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*GVLTQMG DEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRL KNSDDRDSKRDSLEEGELRDHCMEITIRNSPYRR EDSMEDRGEEDDSLAIKPPQQMSRKEKVHHRKD EKRKEKWKHARVKEREHERRKRHREEQDKARRE WERQKRREMAREHSRRERDRLEQLERKRERERKM REQQKEQREQKERERRAEERRKEREARREVSAH HRTMREDYSDKVKASHWSRSPPRPPRERFELGD GRKPVKEEKMEERDLLSDLQDISDSERKTSSAES SSAESGSGSEEEEEEEEEEEEEGSTSEESEEEE EEEEEEEEETGSNSEEASEQSAEEVSEEEMSED EERENENHLLVVPESRFDRDSGESEEAEEEVGEG TPQSSALTEGDYVPDSPALLPIELKQELPKYLP ALQGCRSVEEFQCLNRIEEGTYGVVYRAKDKKT DEIVALKRLKMEKEKEGFPITSLREINTILKAQH PNIVTVREIVVGSNMDKIYIVMNYVEHDLKSLM ETMKQPFLPGEVKTLMIQLLRGVKHLHDNWILH RDLKTSNLLLSHAGILKVGDFGLAREYGSPLKAY TPVVVTQWYRAPELLLGAKEYSTAVDMWSVGCI FGELLTQKPLFPGNSEIDQINKVFKELGTPSEK IWPGYSELPVVKKMTFSEHPYNNLRKRFGALLSD QGFDLMNKFLTYFPGRRISAEDGLKHEYFRETP LPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGGL GYSQLGDDDLKETGFHLTTTNQGASAAGPGFSLK F*RSEWTPSW

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRVF*LKWV MKRTLGK*KL*MKFFRKRNEGRNKRRKQR*NA* KILMTGIPSGIPLRRGS*EITAWRSQ*GTPRIE EKTQWKTEEKKMILWPSNHPSKCLGKKKFITEKM KREKKNGSMLE*KKESTNVGNDIEKNRIKLAGN GKDRREGKWQGSIPGEKGTAWSS*KGSGSGSAR CGSSRRSSGSRRSASGGRRSGARSGRPAGKCLHI TER*ERTTATK*KPATGVAARLGRRGSGSSWET AGSQ*KKRKWKKGTCCPTYRTSATARGRPARPS PRQRNQAQVLRKKRRRRKRRRRKGAPVKNQRRRR RKRKRRRRRPAATLRRHQSSLPKK*VRKK*VKM KNEKMKTTSWLFQSHGSTEIPGRVKKQRKKWVR ERRRAAP*QRATMCPTPLPCCPSSSSRSCPSTCR PCRAAGASRSSSA*TGSRRAPMEWSTEQKTRKQ MKLWL*SG*RWRRRRRASRSRP*GRSTPSSRPS IPTLSPLERLWWAATWTRSTS**TTWSTTSRA*W RP*NSPSCQGR*RP**SSCCGG*NTCTTTGSCT VTSRRPTCC*ATPASSRWVILGWRGSTDPL*RP TPRSW*PSGTAPQSCCLVPRNTPRPWTCGQWAAS SGSC*LRSLCSPGIRKSIRSTKCSRSWGPPVRK SGPATVSSQ*SKR*PSASTPTTTSASASGLCSQ TRASTS*TSS*PTSPGGGSALRTASSMSISARPP SPSTPPCSPRGPPRASSSV*SGAPARGPLREAW ATASWVTTT*RRRASTLPPRTRGPLPRAPASAS SSEGQSGPRHG

Sequence NM_033528

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGTGTTTTAACTCAAATGGGT GATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGATAAAACGCTTAA AAAATTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGGAGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCCCCGTATAGAAG AGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATCACAGAAAAGAT GAAAAGAGAAAAGAAAAATGGAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCGGAAACGACATCGAGAAGAACAGGATAAAGCTCGCCGGGAAT GGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGGGGGAATGATGGCGTGTGCCTCTTCAGGGACCGCTTGGAGCAGTTAGA AAGGAAGCGGGAGCGGGAGCGCAAGATGCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCGGGCGGAGGAGCGGCGCAAGGAGCGG GAGGCCCGCAGGGAAGTGTCTGCACATCACCGAACGATGAGAGAGGACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAGCCCGCCTCGGCCGC CGCGGGAGCGGTTCGAGTTGGGAGACGGCCGGAAGCCAGTAAAAGAAGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACAGGACATCAGCGACAG CGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGAATCAGGCTCAGGTTCTGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGGAGC ACCAGTGAAGAATCAGAGGAGGAGGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGCCGAAGAAG TAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTCCGGGGAGAGTGA AGAAGCAGAGGAAGAAGTGGGTGAGGGAACGCCGCAGAGCAGCGCCCTGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGTTGCCCATCGAGCTC AAGCAGGAGCTGCCCAAGTACCTGCCGGCCCTGCAGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGAGGAGGGCACCTATGGAGTGG TCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAAAGCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCCCTGAGGGA GATCAACACCATCCTCAAGGCCCAGCATCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATCGTGATGAACTAC GTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAGGGGAGGTGAAGACCCTGATGATCCAGCTGCTGCGGGGGGTGAAAC ACCTGCACGACAACTGGATCCTGCACCGTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTTTGGGCTGGCGCG GGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTGCTGCTTGGTGCCAAGGAATACTCCACGGCC GTGGACATGTGGTCAGTGGGCTGCATCTTCGGGGAGCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCAACAAAGTGTTCA AGGAGCTGGGGACCCCCAGTGAGAAAATCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAAAAGATGACCTTCAGCGAGCACCCCTACAACAACCTCCG CAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCATGAACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGCCTC AAGCATGAGTATTTCCGCGAGACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGAAGCGGGGCACCA GCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCTGAAGGAGACGGGCTTCCACCTTACCACCACGAACCAGGGGGCCTC TGCCGCGGGCCCCGGCTTCAGCCTCAAGTTCTGAAGGTCAGAGTGGACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGCFNSNG **KGLLESENFR*NSSGKETKEGTRGESRDKTLK KF**PGFQAGFP*GGGAERSLHGDHNKELPV*K RRLNGRQRRRR*FFGHQTTPANVSERKSSSQKR *KEKRKMEAC*SERKRARTSETTSRRTG*SSPGM GKTEEKGNGKGAFQERKGE*WRVPLQGPLGAVR KEAGAGAQDAGAAEGAAGAEGARAAGGGAAQGA GGPQGSVCTSPNDERGLQRQSESQPLESQPASAA AGAVRVGRRPEASKRRENGRKGPAVRLTGHQRQ REEDQLGRVLVSGIRLRF*GRRGGGGRGGGGRE HQ*RIRGGGGGRGRGGGGDRQQL*GGIRAVCRRS K*GRNE*R*RTRK*KPPLGCSRVTVRPRFRGE* RSRGRSG*GNAAEQRPDRGRLCARLPCPVAHRA QAGAAQVPAGPAGLPERRGVPVPEQDRGGHLWSG LQSKRQENR*NCGSKAAEDGEGEGGLPDHVPEG DQHHPQGPASQHCHR*RDCGGQQHGQDLHRDEL RGARPQEPDGDHETALPARGGEDPDDPAAAGGET PARQLDPAP*PQDVQPAAEPRRHPQGG*FWAGA GVRIPSEGLHPGRGDPVVPRPRAAAWCQGILHG RGHVVSGLHLRGAADSEASVPREFGNRSDQQSVQ GAGDPQ*ENLARLQ*APSSQKDDLQRAPLQQPP QALRGSALRPGLRPHEQVPDLLPREEDQR*GRP QA*VFPRDPPPHRPLHVPHVARQERAAACEAGHQ PEAP*GRPGLQPAG*RRPEGDGLPPYHHEPGGL CRGPRLQPQVLKVRVDPVMG

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*GVLTQMG DEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRL KNSDDRDSKRDSLEEGELRDHCMEITIRNSPYRR EDSMEDRGEEDDSLAIKPPQQMSRKEKVHHRKD EKRKEKWKHARVKEREHERRKRHREEQDKARRE WERQKRREMAREHSRRERGNDGVCLFRDRLEQLE RKRERERKMREQQKEQREQKERERRAEERRKER EARREVSAHHRTMREDYSDKVKASHWSRSPPRP PRERFELGDGRKPVKEEKMEERDLLSDLQDISDS ERKTSSAESSSAESGSGSEEEEEEEEEEEEEGS TSEESEEEEEEEEEEEEETGSNSEEASEQSAEE VSEEEMSEDEERENENHLLVVPESRFDRDSGESE EAEEEVGEGTPQSSALTEGDYVPDSPALLPIEL KQELPKYLPALQGCRSVEEFQCLNRIEEGTYGV VYRAKDKKTDEIVALKRLKMEKEKEGFPITSLRE INTILKAQHPNIVTVREIVVGSNMDKIYIVMNY VEHDLKSLMETMKQPFLPGEVKTLMIQLLRGVK HLHDNWILHRDLKTSNLLLSHAGILKVGDFGLAR EYGSPLKAYTPVVVTQWYRAPELLLGAKEYSTA VDMWSVGCIFGELLTQKPLFPGNSEIDQINKVF KELGTPSEKIWPGYSELPVVKKMTFSEHPYNNLR KRFGALLSDQGFDLMNKFLTYFPGRRISAEDGL KHEYFRETPLPIDPSMFPTWPAKSEQQRVKRGT SPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGAS AAGPGFSLKF*RSEWTPSW

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRVF*LKWV MKRTLGK*KL*MKFFRKRNEGRNKRRKQR*NA* KILMTGIPSGIPLRRGS*EITAWRSQ*GTPRIE EKTQWKTEEKKMILWPSNHPSKCLGKKKFITEKM KREKKNGSMLE*KKESTNVGNDIEKNRIKLAGN GKDRREGKWQGSIPGEKGGMMACASSGTAWSS* KGSGSGSARCGSSRRSSGSRRSASGGRRSGARSG RPAGKCLHITER*ERTTATK*KPATGVAARLGR RGSGSSWETAGSQ*KKRKWKKGTCCPTYRTSAT ARGRPARPSPRQRNQAQVLRKKRRRRKRRRRKGA PVKNQRRRRRKRKRRRRRPAATLRRHQSSLPKK *VRKK*VKMKNEKMKTTSWLFQSHGSTEIPGRV KKQRKKWVRERRRAAP*QRATMCPTPLPCCPSSS SRSCPSTCRPCRAAGASRSSSA*TGSRRAPMEW STEQKTRKQMKLWL*SG*RWRRRRRASRSRP*G RSTPSSRPSIPTLSPLERLWWAATWTRSTS**TT WSTTSRA*WRP*NSPSCQGR*RP**SSCCGG*N TCTTTGSCTVTSRRPTCC*ATPASSRWVILGWR GSTDPL*RPTPRSW*PSGTAPQSCCLVPRNTPRP WTCGQWAASSGSC*LRSLCSPGIRKSIRSTKCS RSWGPPVRKSGPATVSSQ*SKR*PSASTPTTTS ASASGLCSQTRASTS*TSS*PTSPGGGSALRTAS SMSISARPPSPSTPPCSPRGPPRASSSV*SGAP ARGPLREAWATASWVTTT*RRRASTLPPRTRGP LPRAPASASSSEGQSGPRHG

Sequence NM_033531

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGTGTTTTAACTCAAATGGGT GATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGATAAAACGCTTAA AAAATTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGGAGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCCCCGTATAGAAG AGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATCACAGAAAAGAT GAAAAGAGAAAAGAAAAATGGAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCGGAAACGACATCGAGAAGAACAGGATAAAGCTCGCCGGGAAT GGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGGGACCGCTTGGAGCAGTTAGAAAGGAAGCGGGAGCGGGAGCGCAAGAT GCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCGGGCGGAGGAGCGGCGCAAGGAGCGGGAGGCCCGCAGGGAAGTGTCTGCACAT CACCGAACGATGAGAGAGGACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAGCCCGCCTCGGCCGCCGCGGGAGCGGTTCGAGTTGGGAGACG GCCGGAAGCCAGGTGAGGCCAGGCCGGCGCCTGCGCAGAAGCCAGCACAGTTAAAAGAAGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACAGGA CATCAGCGACAGCGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGAATCAGGCTCAGGTTCTGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAG GAGGAAGGGAGCACCAGTGAAGAATCAGAGGAGGAGGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGT CTGCCGAAGAAGTAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTC CGGGGAGAGTGAAGAAGCAGAGGAAGAAGTGGGTGAGGGAACGCCGCAGAGCAGCGCCCTGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGTTG CCCATCGAGCTCAAGCAGGAGCTGCCCAAGTACCTGCCGGCCCTGCAGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGAGGAGGGCA CCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAAAGCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCAC GTCCCTGAGGGAGATCAACACCATCCTCAAGGCCCAGCATCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATC GTGATGAACTACGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAGGGGAGGTGAAGACCCTGATGATCCAGCTGCTGC GGGGGGTGAAACACCTGCACGACAACTGGATCCTGCACCGTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTT TGGGCTGGCGCGGGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTGCTGCTTGGTGCCAAGGAA TACTCCACGGCCGTGGACATGTGGTCAGTGGGCTGCATCTTCGGGGAGCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCA ACAAAGTGTTCAAGGAGCTGGGGACCCCCAGTGAGAAAATCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAAAAGATGACCTTCAGCGAGCACCCCTA CAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCATGAACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCT GAGGACGGCCTCAAGCATGAGTATTTCCGCGAGACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGA AGCGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCTGAAGGAGACGGGCTTCCACCTTACCACCACGAA CCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTCAAGTTCTGAAGGTCAGAGTGGACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGCFNSNG **KGLLESENFR*NSSGKETKEGTRGESRDKTLK KF**PGFQAGFP*GGGAERSLHGDHNKELPV*K RRLNGRQRRRR*FFGHQTTPANVSERKSSSQKR *KEKRKMEAC*SERKRARTSETTSRRTG*SSPGM GKTEEKGNGKGAFQERKGPLGAVRKEAGAGAQD AGAAEGAAGAEGARAAGGGAAQGAGGPQGSVCT SPNDERGLQRQSESQPLESQPASAAAGAVRVGRR PEAR*GQAGACAEASTVKRRENGRKGPAVRLTG HQRQREEDQLGRVLVSGIRLRF*GRRGGGGRGG GGREHQ*RIRGGGGGRGRGGGGDRQQL*GGIRAV CRRSK*GRNE*R*RTRK*KPPLGCSRVTVRPRF RGE*RSRGRSG*GNAAEQRPDRGRLCARLPCPV AHRAQAGAAQVPAGPAGLPERRGVPVPEQDRGGH LWSGLQSKRQENR*NCGSKAAEDGEGEGGLPDH VPEGDQHHPQGPASQHCHR*RDCGGQQHGQDLH RDELRGARPQEPDGDHETALPARGGEDPDDPAAA GGETPARQLDPAP*PQDVQPAAEPRRHPQGG*F WAGAGVRIPSEGLHPGRGDPVVPRPRAAAWCQG ILHGRGHVVSGLHLRGAADSEASVPREFGNRSDQ QSVQGAGDPQ*ENLARLQ*APSSQKDDLQRAPL QQPPQALRGSALRPGLRPHEQVPDLLPREEDQR *GRPQA*VFPRDPPPHRPLHVPHVARQERAAACE AGHQPEAP*GRPGLQPAG*RRPEGDGLPPYHHE PGGLCRGPRLQPQVLKVRVDPVMG

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*GVLTQMG DEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRL KNSDDRDSKRDSLEEGELRDHCMEITIRNSPYRR EDSMEDRGEEDDSLAIKPPQQMSRKEKVHHRKD EKRKEKWKHARVKEREHERRKRHREEQDKARRE WERQKRREMAREHSRRERDRLEQLERKRERERKM REQQKEQREQKERERRAEERRKEREARREVSAH HRTMREDYSDKVKASHWSRSPPRPPRERFELGD GRKPGEARPAPAQKPAQLKEEKMEERDLLSDLQD ISDSERKTSSAESSSAESGSGSEEEEEEEEEEE EEGSTSEESEEEEEEEEEEEEETGSNSEEASEQ SAEEVSEEEMSEDEERENENHLLVVPESRFDRDS GESEEAEEEVGEGTPQSSALTEGDYVPDSPALL PIELKQELPKYLPALQGCRSVEEFQCLNRIEEG TYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPIT SLREINTILKAQHPNIVTVREIVVGSNMDKIYI VMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLL RGVKHLHDNWILHRDLKTSNLLLSHAGILKVGDF GLAREYGSPLKAYTPVVVTQWYRAPELLLGAKE YSTAVDMWSVGCIFGELLTQKPLFPGNSEIDQI NKVFKELGTPSEKIWPGYSELPVVKKMTFSEHPY NNLRKRFGALLSDQGFDLMNKFLTYFPGRRISA EDGLKHEYFRETPLPIDPSMFPTWPAKSEQQRV KRGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTN QGASAAGPGFSLKF*RSEWTPSW

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRVF*LKWV MKRTLGK*KL*MKFFRKRNEGRNKRRKQR*NA* KILMTGIPSGIPLRRGS*EITAWRSQ*GTPRIE EKTQWKTEEKKMILWPSNHPSKCLGKKKFITEKM KREKKNGSMLE*KKESTNVGNDIEKNRIKLAGN GKDRREGKWQGSIPGEKGTAWSS*KGSGSGSAR CGSSRRSSGSRRSASGGRRSGARSGRPAGKCLHI TER*ERTTATK*KPATGVAARLGRRGSGSSWET AGSQVRPGRRLRRSQHS*KKRKWKKGTCCPTYR TSATARGRPARPSPRQRNQAQVLRKKRRRRKRRR RKGAPVKNQRRRRRKRKRRRRRPAATLRRHQSS LPKK*VRKK*VKMKNEKMKTTSWLFQSHGSTEI PGRVKKQRKKWVRERRRAAP*QRATMCPTPLPCC PSSSSRSCPSTCRPCRAAGASRSSSA*TGSRRA PMEWSTEQKTRKQMKLWL*SG*RWRRRRRASRS RP*GRSTPSSRPSIPTLSPLERLWWAATWTRSTS **TTWSTTSRA*WRP*NSPSCQGR*RP**SSCC GG*NTCTTTGSCTVTSRRPTCC*ATPASSRWVI LGWRGSTDPL*RPTPRSW*PSGTAPQSCCLVPRN TPRPWTCGQWAASSGSC*LRSLCSPGIRKSIRS TKCSRSWGPPVRKSGPATVSSQ*SKR*PSASTP TTTSASASGLCSQTRASTS*TSS*PTSPGGGSAL RTASSMSISARPPSPSTPPCSPRGPPRASSSV* SGAPARGPLREAWATASWVTTT*RRRASTLPPR TRGPLPRAPASASSSEGQSGPRHG

Sequence NM_033489

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGGTGAGCGGCGGTGTTTTAA CTCAAATGGGTGATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGAT AAAACGCTTAAAAAATAACAACGCTTCTTCGGTGAAGTTCTTTTGTACTTCCAAATGTCGCAGTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGG AGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCCCCGTATAGAAGAGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTT GGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATCACAGAAAAGATGAAAAGAGAAAAGAAAAATGTAGGCATCATAGCCATTCAGCA GAAGGGGGGAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCGGAAACGACATCGAGAAGAACAGGATAAAGCTCGCCGGGAATGGGAAAGACAGA AGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGGGACCGCTTGGAGCAGTTAGAAAGGAAGCGGGAGCGGGAGCGCAAGATGCGGGAGCAGCA GAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCGGGCGGAGGAGCGGCGCAAGGAGCGGGAGGCCCGCAGGGAAGTGTCTGCACATCACCGAACGATG AGAGAGGACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAGCCCGCCTCGGCCGCCGCGGGAGCGGTTCGAGTTGGGAGACGGCCGGAAGCCAG TAAAAGAAGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACAGGACATCAGCGACAGCGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGA ATCAGGCTCAGGTTCTGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGGAGCACCAGTGAAGAATCAGAGGAGGAGGAGGAGGAAGAGGAA GAGGAGGAGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGCCGAAGAAGTAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAA ATGAAAACCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTCCGGGGAGAGTGAAGAAGCAGAGGAAGAAGTGGGTGAGGGAACGCCGCAGAG CAGCGCCCTGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGTTGCCCATCGAGCTCAAGCAGGAGCTGCCCAAGTACCTGCCGGCCCTGCAGGGC TGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGAGGAGGGCACCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGG CTCTAAAGCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCCCTGAGGGAGATCAACACCATCCTCAAGGCCCAGCATCCCAACATTGT CACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATCGTGATGAACTACGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAA CAGCCCTTCCTGCCAGGGGAGGTGAAGACCCTGATGATCCAGCTGCTGCGGGGGGTGAAACACCTGCACGACAACTGGATCCTGCACCGTGACCTCAAGA CGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTTTGGGCTGGCGCGGGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGT GGTGACCCAGTGGTACCGCGCCCCAGAGCTGCTGCTTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGTCAGTGGGCTGCATCTTCGGGGAGCTG CTGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCAACAAAGTGTTCAAGGAGCTGGGGACCCCCAGTGAGAAAATCTGGCCCGGCT ACAGTGAGCTCCCAGTAGTCAAAAAGATGACCTTCAGCGAGCACCCCTACAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGA CCTCATGAACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATTTCCGCGAGACCCCCCTCCCCATCGAC CCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGAAGCGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGC TGGGTGACGACGACCTGAAGGAGACGGGCTTCCACCTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTCAAGTTCTGAAGGTC AGAGTGGACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGVSGGVL TQMGDEKDSWKVKTLDEILQEKKRRKEQEEKAEI KRLKNNNASSVKFFCTSKCRSLMTGIPSGIPLR RGS*EITAWRSQ*GTPRIEEKTQWKTEEKKMIL WPSNHPSKCLGKKKFITEKMKREKKNVGIIAIQQ KGGSMLE*KKESTNVGNDIEKNRIKLAGNGKDR REGKWQGSIPGEKGTAWSS*KGSGSGSARCGSS RRSSGSRRSASGGRRSGARSGRPAGKCLHITER* ERTTATK*KPATGVAARLGRRGSGSSWETAGSQ *KKRKWKKGTCCPTYRTSATARGRPARPSPRQR NQAQVLRKKRRRRKRRRRKGAPVKNQRRRRRKRK RRRRRPAATLRRHQSSLPKK*VRKK*VKMKNEK MKTTSWLFQSHGSTEIPGRVKKQRKKWVRERRR AAP*QRATMCPTPLPCCPSSSSRSCPSTCRPCRA AGASRSSSA*TGSRRAPMEWSTEQKTRKQMKLW L*SG*RWRRRRRASRSRP*GRSTPSSRPSIPTL SPLERLWWAATWTRSTS**TTWSTTSRA*WRP*N SPSCQGR*RP**SSCCGG*NTCTTTGSCTVTSR RPTCC*ATPASSRWVILGWRGSTDPL*RPTPRS W*PSGTAPQSCCLVPRNTPRPWTCGQWAASSGSC *LRSLCSPGIRKSIRSTKCSRSWGPPVRKSGPA TVSSQ*SKR*PSASTPTTTSASASGLCSQTRAS TS*TSS*PTSPGGGSALRTASSMSISARPPSPST PPCSPRGPPRASSSV*SGAPARGPLREAWATAS WVTTT*RRRASTLPPRTRGPLPRAPASASSSEG QSGPRHG

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*G*AAVF* LKWVMKRTLGK*KL*MKFFRKRNEGRNKRRKQR *NA*KITTLLR*SSFVLPNVAV**PGFQAGFP*G GGAERSLHGDHNKELPV*KRRLNGRQRRRR*FF GHQTTPANVSERKSSSQKR*KEKRKM*AS*PFS RRGEAC*SERKRARTSETTSRRTG*SSPGMGKTE EKGNGKGAFQERKGPLGAVRKEAGAGAQDAGAA EGAAGAEGARAAGGGAAQGAGGPQGSVCTSPND ERGLQRQSESQPLESQPASAAAGAVRVGRRPEAS KRRENGRKGPAVRLTGHQRQREEDQLGRVLVSG IRLRF*GRRGGGGRGGGGREHQ*RIRGGGGGRG RGGGGDRQQL*GGIRAVCRRSK*GRNE*R*RTRK *KPPLGCSRVTVRPRFRGE*RSRGRSG*GNAAE QRPDRGRLCARLPCPVAHRAQAGAAQVPAGPAG LPERRGVPVPEQDRGGHLWSGLQSKRQENR*NCG SKAAEDGEGEGGLPDHVPEGDQHHPQGPASQHC HR*RDCGGQQHGQDLHRDELRGARPQEPDGDHE TALPARGGEDPDDPAAAGGETPARQLDPAP*PQD VQPAAEPRRHPQGG*FWAGAGVRIPSEGLHPGR GDPVVPRPRAAAWCQGILHGRGHVVSGLHLRGA ADSEASVPREFGNRSDQQSVQGAGDPQ*ENLARL Q*APSSQKDDLQRAPLQQPPQALRGSALRPGLR PHEQVPDLLPREEDQR*GRPQA*VFPRDPPPHR PLHVPHVARQERAAACEAGHQPEAP*GRPGLQPA G*RRPEGDGLPPYHHEPGGLCRGPRLQPQVLKV RVDPVMG

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRGERRCFN SNG**KGLLESENFR*NSSGKETKEGTRGESRD KTLKK*QRFFGEVLLYFQMSQSDDRDSKRDSLE EGELRDHCMEITIRNSPYRREDSMEDRGEEDDSL AIKPPQQMSRKEKVHHRKDEKRKEKCRHHSHSA EGGKHARVKEREHERRKRHREEQDKARREWERQ KRREMAREHSRRERDRLEQLERKRERERKMREQQ KEQREQKERERRAEERRKEREARREVSAHHRTM REDYSDKVKASHWSRSPPRPPRERFELGDGRKP VKEEKMEERDLLSDLQDISDSERKTSSAESSSAE SGSGSEEEEEEEEEEEEEGSTSEESEEEEEEEE EEEEETGSNSEEASEQSAEEVSEEEMSEDEERE NENHLLVVPESRFDRDSGESEEAEEEVGEGTPQS SALTEGDYVPDSPALLPIELKQELPKYLPALQG CRSVEEFQCLNRIEEGTYGVVYRAKDKKTDEIV ALKRLKMEKEKEGFPITSLREINTILKAQHPNIV TVREIVVGSNMDKIYIVMNYVEHDLKSLMETMK QPFLPGEVKTLMIQLLRGVKHLHDNWILHRDLK TSNLLLSHAGILKVGDFGLAREYGSPLKAYTPVV VTQWYRAPELLLGAKEYSTAVDMWSVGCIFGEL LTQKPLFPGNSEIDQINKVFKELGTPSEKIWPG YSELPVVKKMTFSEHPYNNLRKRFGALLSDQGFD LMNKFLTYFPGRRISAEDGLKHEYFRETPLPID PSMFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQ LGDDDLKETGFHLTTTNQGASAAGPGFSLKF*RS EWTPSW

Sequence NM_033490

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGGTGAGCGGCGGTGTTTTAA CTCAAATGGGTGATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGAT AAAACGCTTAAAAAATTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGGAGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCC CCGTATAGAAGAGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATC ACAGAAAAGATGAAAAGAGAAAAGAAAAATGTAGGCATCATAGCCATTCAGCAGAAGGGGGGAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCG GAAACGACATCGAGAAGAACAGGATAAAGCTCGCCGGGAATGGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGTGTCTGC ACATCACCGAACGATGAGAGAGGACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAGCCCGCCTCGGCCGCCGCGGGAGCGGTTCGAGTTGGGA GACGGCCGGAAGCCAGTAAAAGAAGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACAGGACATCAGCGACAGCGAGAGGAAGACCAGCTCGGCCG AGTCCTCGTCAGCGGAATCAGGCTCAGGTTCTGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGGAGCACCAGTGAAGAATCAGAGGAGGA GGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGCCGAAGAAGTAAGTGAGGAAGAAATGAGTGAA GATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTCCGGGGAGAGTGAAGAAGCAGAGGAAGAAGTGGGTG AGGGAACGCCGCAGAGCAGCGCCCTGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGTTGCCCATCGAGCTCAAGCAGGAGCTGCCCAAGTACCT GCCGGCCCTGCAGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGAGGAGGGCACCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAA ACAGATGAAATTGTGGCTCTAAAGCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCCCTGAGGGAGATCAACACCATCCTCAAGGCCC AGCATCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATCGTGATGAACTACGTGGAGCACGACCTCAAGAGCCT GATGGAGACCATGAAACAGCCCTTCCTGCCAGGGGAGGTGAAGACCCTGATGATCCAGCTGCTGCGGGGGGTGAAACACCTGCACGACAACTGGATCCTG CACCGTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTTTGGGCTGGCGCGGGAGTACGGATCCCCTCTGAAGG CCTACACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTGCTGCTTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGTCAGTGGGCTG CATCTTCGGGGAGCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCAACAAAGTGTTCAAGGAGCTGGGGACCCCCAGTGAG AAAATCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAAAAGATGACCTTCAGCGAGCACCCCTACAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCT CAGACCAGGGCTTCGACCTCATGAACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATTTCCGCGAGAC CCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGAAGCGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGC CTGGGCTACAGCCAGCTGGGTGACGACGACCTGAAGGAGACGGGCTTCCACCTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCC TCAAGTTCTGAAGGTCAGAGTGGACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGVSGGVL TQMGDEKDSWKVKTLDEILQEKKRRKEQEEKAEI KRLKNSDDRDSKRDSLEEGELRDHCMEITIRNS PYRREDSMEDRGEEDDSLAIKPPQQMSRKEKVH HRKDEKRKEKCRHHSHSAEGGKHARVKEREHERR KRHREEQDKARREWERQKRREMAREHSRRESVC TSPNDERGLQRQSESQPLESQPASAAAGAVRVG RRPEASKRRENGRKGPAVRLTGHQRQREEDQLGR VLVSGIRLRF*GRRGGGGRGGGGREHQ*RIRGG GGGRGRGGGGDRQQL*GGIRAVCRRSK*GRNE* R*RTRK*KPPLGCSRVTVRPRFRGE*RSRGRSG* GNAAEQRPDRGRLCARLPCPVAHRAQAGAAQVP AGPAGLPERRGVPVPEQDRGGHLWSGLQSKRQE NR*NCGSKAAEDGEGEGGLPDHVPEGDQHHPQGP ASQHCHR*RDCGGQQHGQDLHRDELRGARPQEP DGDHETALPARGGEDPDDPAAAGGETPARQLDP AP*PQDVQPAAEPRRHPQGG*FWAGAGVRIPSEG LHPGRGDPVVPRPRAAAWCQGILHGRGHVVSGL HLRGAADSEASVPREFGNRSDQQSVQGAGDPQ* ENLARLQ*APSSQKDDLQRAPLQQPPQALRGSAL RPGLRPHEQVPDLLPREEDQR*GRPQA*VFPRD PPPHRPLHVPHVARQERAAACEAGHQPEAP*GR PGLQPAG*RRPEGDGLPPYHHEPGGLCRGPRLQP QVLKVRVDPVMG

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*G*AAVF* LKWVMKRTLGK*KL*MKFFRKRNEGRNKRRKQR *NA*KILMTGIPSGIPLRRGS*EITAWRSQ*GTP RIEEKTQWKTEEKKMILWPSNHPSKCLGKKKFI TEKMKREKKNVGIIAIQQKGGSMLE*KKESTNV GNDIEKNRIKLAGNGKDRREGKWQGSIPGEKVSA HHRTMREDYSDKVKASHWSRSPPRPPRERFELG DGRKPVKEEKMEERDLLSDLQDISDSERKTSSA ESSSAESGSGSEEEEEEEEEEEEEGSTSEESEEE EEEEEEEEEETGSNSEEASEQSAEEVSEEEMSE DEERENENHLLVVPESRFDRDSGESEEAEEEVG EGTPQSSALTEGDYVPDSPALLPIELKQELPKYL PALQGCRSVEEFQCLNRIEEGTYGVVYRAKDKK TDEIVALKRLKMEKEKEGFPITSLREINTILKA QHPNIVTVREIVVGSNMDKIYIVMNYVEHDLKSL METMKQPFLPGEVKTLMIQLLRGVKHLHDNWIL HRDLKTSNLLLSHAGILKVGDFGLAREYGSPLK AYTPVVVTQWYRAPELLLGAKEYSTAVDMWSVGC IFGELLTQKPLFPGNSEIDQINKVFKELGTPSE KIWPGYSELPVVKKMTFSEHPYNNLRKRFGALL SDQGFDLMNKFLTYFPGRRISAEDGLKHEYFRET PLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEGG LGYSQLGDDDLKETGFHLTTTNQGASAAGPGFS LKF*RSEWTPSW

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRGERRCFN SNG**KGLLESENFR*NSSGKETKEGTRGESRD KTLKKF**PGFQAGFP*GGGAERSLHGDHNKEL PV*KRRLNGRQRRRR*FFGHQTTPANVSERKSSS QKR*KEKRKM*AS*PFSRRGEAC*SERKRARTS ETTSRRTG*SSPGMGKTEEKGNGKGAFQERKCL HITER*ERTTATK*KPATGVAARLGRRGSGSSWE TAGSQ*KKRKWKKGTCCPTYRTSATARGRPARP SPRQRNQAQVLRKKRRRRKRRRRKGAPVKNQRR RRRKRKRRRRRPAATLRRHQSSLPKK*VRKK*VK MKNEKMKTTSWLFQSHGSTEIPGRVKKQRKKWV RERRRAAP*QRATMCPTPLPCCPSSSSRSCPST CRPCRAAGASRSSSA*TGSRRAPMEWSTEQKTRK QMKLWL*SG*RWRRRRRASRSRP*GRSTPSSRP SIPTLSPLERLWWAATWTRSTS**TTWSTTSRA *WRP*NSPSCQGR*RP**SSCCGG*NTCTTTGSC TVTSRRPTCC*ATPASSRWVILGWRGSTDPL*R PTPRSW*PSGTAPQSCCLVPRNTPRPWTCGQWA ASSGSC*LRSLCSPGIRKSIRSTKCSRSWGPPVR KSGPATVSSQ*SKR*PSASTPTTTSASASGLCS QTRASTS*TSS*PTSPGGGSALRTASSMSISAR PPSPSTPPCSPRGPPRASSSV*SGAPARGPLREA WATASWVTTT*RRRASTLPPRTRGPLPRAPASA SSSEGQSGPRHG

Sequence NM_033488

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGGTGAGCGGCGGTGTTTTAA CTCAAATGGGTGATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGAT AAAACGCTTAAAAAATAACAACGCTTCTTCGGTGAAGTTCTTTTGTACTTCCAAATGTCGCAGTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGG AGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCCCCGTATAGAAGAGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTT GGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATCACAGAAAAGATGAAAAGAGAAAAGAAAAAAAGCATGCTAGAGTGAAAGAAAGA GAGCACGAACGTCGGAAACGACATCGAGAAGAACAGGATAAAGCTCGCCGGGAATGGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGA GAGAAAGGGACCGCTTGGAGCAGTTAGAAAGGAAGCGGGAGCGGGAGCGCAAGATGCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCG GCGGGCGGAGGAGCGGCGCAAGGAGCGGGAGGCCCGCAGGGAAGTGTCTGCACATCACCGAACGATGAGAGAGGACTACAGCGACAAAGTGAAAGCCAGC CACTGGAGTCGCAGCCCGCCTCGGCCGCCGCGGGAGCGGTTCGAGTTGGGAGACGGCCGGAAGCCAGTAAAAGAAGAGAAAATGGAAGAAAGGGACCTGC TGTCCGACTTACAGGACATCAGCGACAGCGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGAATCAGGCTCAGGTTCTGAGGAAGAAGAGGAGGA GGAGGAAGAGGAGGAGGAGGAAGGGAGCACCAGTGAAGAATCAGAGGAGGAGGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGACCGGCAGCAACTCTGAG GAGGCATCAGAGCAGTCTGCCGAAGAAGTAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCAGAGTCAC GGTTCGACCGAGATTCCGGGGAGAGTGAAGAAGCAGAGGAAGAAGTGGGTGAGGGAACGCCGCAGAGCAGCGCCCTGACAGAGGGCGACTATGTGCCCGA CTCCCCTGCCCTGTTGCCCATCGAGCTCAAGCAGGAGCTGCCCAAGTACCTGCCGGCCCTGCAGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAAC AGGATCGAGGAGGGCACCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAAAGCGGCTGAAGATGGAGAAGGAGAAGG AGGGCTTCCCGATCACGTCCCTGAGGGAGATCAACACCATCCTCAAGGCCCAGCATCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACAT GGACAAGATCTACATCGTGATGAACTACGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAGGGGAGGTGAAGACCCTG ATGATCCAGCTGCTGCGGGGGGTGAAACACCTGCACGACAACTGGATCCTGCACCGTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCC TCAAGGTGGGTGATTTTGGGCTGGCGCGGGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTGCT GCTTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGTCAGTGGGCTGCATCTTCGGGGAGCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAATTCG GAAATCGATCAGATCAACAAAGTGTTCAAGGAGCTGGGGACCCCCAGTGAGAAAATCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAAAAGATGACCT TCAGCGAGCACCCCTACAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCATGAACAAGTTCCTGACCTACTTCCCCGG GAGGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATTTCCGCGAGACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGC GAGCAGCAGCGTGTGAAGCGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCTGAAGGAGACGGGCTTCC ACCTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTCAAGTTCTGAAGGTCAGAGTGGACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGVSGGVL TQMGDEKDSWKVKTLDEILQEKKRRKEQEEKAEI KRLKNNNASSVKFFCTSKCRSLMTGIPSGIPLR RGS*EITAWRSQ*GTPRIEEKTQWKTEEKKMIL WPSNHPSKCLGKKKFITEKMKREKKKSMLE*KKE STNVGNDIEKNRIKLAGNGKDRREGKWQGSIPG EKGTAWSS*KGSGSGSARCGSSRRSSGSRRSAS GGRRSGARSGRPAGKCLHITER*ERTTATK*KPA TGVAARLGRRGSGSSWETAGSQ*KKRKWKKGTC CPTYRTSATARGRPARPSPRQRNQAQVLRKKRR RRKRRRRKGAPVKNQRRRRRKRKRRRRRPAATLR RHQSSLPKK*VRKK*VKMKNEKMKTTSWLFQSH GSTEIPGRVKKQRKKWVRERRRAAP*QRATMCP TPLPCCPSSSSRSCPSTCRPCRAAGASRSSSA*T GSRRAPMEWSTEQKTRKQMKLWL*SG*RWRRRR RASRSRP*GRSTPSSRPSIPTLSPLERLWWAAT WTRSTS**TTWSTTSRA*WRP*NSPSCQGR*RP* *SSCCGG*NTCTTTGSCTVTSRRPTCC*ATPAS SRWVILGWRGSTDPL*RPTPRSW*PSGTAPQSC CLVPRNTPRPWTCGQWAASSGSC*LRSLCSPGIR KSIRSTKCSRSWGPPVRKSGPATVSSQ*SKR*P SASTPTTTSASASGLCSQTRASTS*TSS*PTSP GGGSALRTASSMSISARPPSPSTPPCSPRGPPRA SSSV*SGAPARGPLREAWATASWVTTT*RRRAS TLPPRTRGPLPRAPASASSSEGQSGPRHG

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*G*AAVF* LKWVMKRTLGK*KL*MKFFRKRNEGRNKRRKQR *NA*KITTLLR*SSFVLPNVAV**PGFQAGFP*G GGAERSLHGDHNKELPV*KRRLNGRQRRRR*FF GHQTTPANVSERKSSSQKR*KEKRKKAC*SERK RARTSETTSRRTG*SSPGMGKTEEKGNGKGAFQE RKGPLGAVRKEAGAGAQDAGAAEGAAGAEGARA AGGGAAQGAGGPQGSVCTSPNDERGLQRQSESQ PLESQPASAAAGAVRVGRRPEASKRRENGRKGPA VRLTGHQRQREEDQLGRVLVSGIRLRF*GRRGG GGRGGGGREHQ*RIRGGGGGRGRGGGGDRQQL* GGIRAVCRRSK*GRNE*R*RTRK*KPPLGCSRVT VRPRFRGE*RSRGRSG*GNAAEQRPDRGRLCAR LPCPVAHRAQAGAAQVPAGPAGLPERRGVPVPE QDRGGHLWSGLQSKRQENR*NCGSKAAEDGEGEG GLPDHVPEGDQHHPQGPASQHCHR*RDCGGQQH GQDLHRDELRGARPQEPDGDHETALPARGGEDP DDPAAAGGETPARQLDPAP*PQDVQPAAEPRRHP QGG*FWAGAGVRIPSEGLHPGRGDPVVPRPRAA AWCQGILHGRGHVVSGLHLRGAADSEASVPREF GNRSDQQSVQGAGDPQ*ENLARLQ*APSSQKDDL QRAPLQQPPQALRGSALRPGLRPHEQVPDLLPR EEDQR*GRPQA*VFPRDPPPHRPLHVPHVARQE RAAACEAGHQPEAP*GRPGLQPAG*RRPEGDGLP PYHHEPGGLCRGPRLQPQVLKVRVDPVMG

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRGERRCFN SNG**KGLLESENFR*NSSGKETKEGTRGESRD KTLKK*QRFFGEVLLYFQMSQSDDRDSKRDSLE EGELRDHCMEITIRNSPYRREDSMEDRGEEDDSL AIKPPQQMSRKEKVHHRKDEKRKEKKHARVKER EHERRKRHREEQDKARREWERQKRREMAREHSR RERDRLEQLERKRERERKMREQQKEQREQKERER RAEERRKEREARREVSAHHRTMREDYSDKVKAS HWSRSPPRPPRERFELGDGRKPVKEEKMEERDL LSDLQDISDSERKTSSAESSSAESGSGSEEEEEE EEEEEEEGSTSEESEEEEEEEEEEEEETGSNSE EASEQSAEEVSEEEMSEDEERENENHLLVVPES RFDRDSGESEEAEEEVGEGTPQSSALTEGDYVPD SPALLPIELKQELPKYLPALQGCRSVEEFQCLN RIEEGTYGVVYRAKDKKTDEIVALKRLKMEKEK EGFPITSLREINTILKAQHPNIVTVREIVVGSNM DKIYIVMNYVEHDLKSLMETMKQPFLPGEVKTL MIQLLRGVKHLHDNWILHRDLKTSNLLLSHAGI LKVGDFGLAREYGSPLKAYTPVVVTQWYRAPELL LGAKEYSTAVDMWSVGCIFGELLTQKPLFPGNS EIDQINKVFKELGTPSEKIWPGYSELPVVKKMT FSEHPYNNLRKRFGALLSDQGFDLMNKFLTYFPG RRISAEDGLKHEYFRETPLPIDPSMFPTWPAKS EQQRVKRGTSPRPPEGGLGYSQLGDDDLKETGF HLTTTNQGASAAGPGFSLKF*RSEWTPSW

Sequence NM_033487

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGGTGAGCGGCGGTGTTTTAA CTCAAATGGGTGATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGAT AAAACGCTTAAAAAATTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGGAGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCC CCGTATAGAAGAGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATC ACAGAAAAGATGAAAAGAGAAAAGAAAAATGTAGGCATCATAGCCATTCAGCAGAAGGGGGGAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCG GAAACGACATCGAGAAGAACAGGATAAAGCTCGCCGGGAATGGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGTAAAAGA AGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACAGGACATCAGCGACAGCGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGAATCAGGC TCAGGTTCTGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGGAGCACCAGTGAAGAATCAGAGGAGGAGGAGGAGGAAGAGGAAGAGGAGG AGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGCCGAAGAAGTAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAAATGAAAA CCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTCCGGGGAGAGTGAAGAAGCAGAGGAAGAAGTGGGTGAGGGAACGCCGCAGAGCAGCGCC CTGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGTTGCCCATCGAGCTCAAGCAGGAGCTGCCCAAGTACCTGCCGGCCCTGCAGGGCTGCCGGA GCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGAGGAGGGCACCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAAA GCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCCCTGAGGGAGATCAACACCATCCTCAAGGCCCAGCATCCCAACATTGTCACCGTT AGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATCGTGATGAACTACGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCT TCCTGCCAGGGGAGGTGAAGACCCTGATGATCCAGCTGCTGCGGGGGGTGAAACACCTGCACGACAACTGGATCCTGCACCGTGACCTCAAGACGTCCAA CCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTTTGGGCTGGCGCGGGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACC CAGTGGTACCGCGCCCCAGAGCTGCTGCTTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGTCAGTGGGCTGCATCTTCGGGGAGCTGCTGACTC AGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCAACAAAGTGTTCAAGGAGCTGGGGACCCCCAGTGAGAAAATCTGGCCCGGCTACAGTGA GCTCCCAGTAGTCAAAAAGATGACCTTCAGCGAGCACCCCTACAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCATG AACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATTTCCGCGAGACCCCCCTCCCCATCGACCCCTCCA TGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGAAGCGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGA CGACGACCTGAAGGAGACGGGCTTCCACCTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTCAAGTTCTGAAGGTCAGAGTGG ACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGVSGGVL TQMGDEKDSWKVKTLDEILQEKKRRKEQEEKAEI KRLKNSDDRDSKRDSLEEGELRDHCMEITIRNS PYRREDSMEDRGEEDDSLAIKPPQQMSRKEKVH HRKDEKRKEKCRHHSHSAEGGKHARVKEREHERR KRHREEQDKARREWERQKRREMAREHSRRESKR RENGRKGPAVRLTGHQRQREEDQLGRVLVSGIR LRF*GRRGGGGRGGGGREHQ*RIRGGGGGRGRGG GGDRQQL*GGIRAVCRRSK*GRNE*R*RTRK*K PPLGCSRVTVRPRFRGE*RSRGRSG*GNAAEQR PDRGRLCARLPCPVAHRAQAGAAQVPAGPAGLPE RRGVPVPEQDRGGHLWSGLQSKRQENR*NCGSK AAEDGEGEGGLPDHVPEGDQHHPQGPASQHCHR *RDCGGQQHGQDLHRDELRGARPQEPDGDHETAL PARGGEDPDDPAAAGGETPARQLDPAP*PQDVQ PAAEPRRHPQGG*FWAGAGVRIPSEGLHPGRGD PVVPRPRAAAWCQGILHGRGHVVSGLHLRGAADS EASVPREFGNRSDQQSVQGAGDPQ*ENLARLQ* APSSQKDDLQRAPLQQPPQALRGSALRPGLRPH EQVPDLLPREEDQR*GRPQA*VFPRDPPPHRPLH VPHVARQERAAACEAGHQPEAP*GRPGLQPAG* RRPEGDGLPPYHHEPGGLCRGPRLQPQVLKVRV DPVMG

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*G*AAVF* LKWVMKRTLGK*KL*MKFFRKRNEGRNKRRKQR *NA*KILMTGIPSGIPLRRGS*EITAWRSQ*GTP RIEEKTQWKTEEKKMILWPSNHPSKCLGKKKFI TEKMKREKKNVGIIAIQQKGGSMLE*KKESTNV GNDIEKNRIKLAGNGKDRREGKWQGSIPGEKVKE EKMEERDLLSDLQDISDSERKTSSAESSSAESG SGSEEEEEEEEEEEEEGSTSEESEEEEEEEEEE EEETGSNSEEASEQSAEEVSEEEMSEDEERENEN HLLVVPESRFDRDSGESEEAEEEVGEGTPQSSA LTEGDYVPDSPALLPIELKQELPKYLPALQGCR SVEEFQCLNRIEEGTYGVVYRAKDKKTDEIVALK RLKMEKEKEGFPITSLREINTILKAQHPNIVTV REIVVGSNMDKIYIVMNYVEHDLKSLMETMKQP FLPGEVKTLMIQLLRGVKHLHDNWILHRDLKTSN LLLSHAGILKVGDFGLAREYGSPLKAYTPVVVT QWYRAPELLLGAKEYSTAVDMWSVGCIFGELLT QKPLFPGNSEIDQINKVFKELGTPSEKIWPGYSE LPVVKKMTFSEHPYNNLRKRFGALLSDQGFDLM NKFLTYFPGRRISAEDGLKHEYFRETPLPIDPS MFPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGD DDLKETGFHLTTTNQGASAAGPGFSLKF*RSEW TPSW

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRGERRCFN SNG**KGLLESENFR*NSSGKETKEGTRGESRD KTLKKF**PGFQAGFP*GGGAERSLHGDHNKEL PV*KRRLNGRQRRRR*FFGHQTTPANVSERKSSS QKR*KEKRKM*AS*PFSRRGEAC*SERKRARTS ETTSRRTG*SSPGMGKTEEKGNGKGAFQERK*K KRKWKKGTCCPTYRTSATARGRPARPSPRQRNQA QVLRKKRRRRKRRRRKGAPVKNQRRRRRKRKRR RRRPAATLRRHQSSLPKK*VRKK*VKMKNEKMK TTSWLFQSHGSTEIPGRVKKQRKKWVRERRRAAP *QRATMCPTPLPCCPSSSSRSCPSTCRPCRAAG ASRSSSA*TGSRRAPMEWSTEQKTRKQMKLWL* SG*RWRRRRRASRSRP*GRSTPSSRPSIPTLSPL ERLWWAATWTRSTS**TTWSTTSRA*WRP*NSP SCQGR*RP**SSCCGG*NTCTTTGSCTVTSRRP TCC*ATPASSRWVILGWRGSTDPL*RPTPRSW*P SGTAPQSCCLVPRNTPRPWTCGQWAASSGSC*L RSLCSPGIRKSIRSTKCSRSWGPPVRKSGPATV SSQ*SKR*PSASTPTTTSASASGLCSQTRASTS* TSS*PTSPGGGSALRTASSMSISARPPSPSTPP CSPRGPPRASSSV*SGAPARGPLREAWATASWV TTT*RRRASTLPPRTRGPLPRAPASASSSEGQSG PRHG

Sequence NM_001787

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGGTGAGCGGCGGTGTTTTAA CTCAAATGGGTGATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGAT AAAACGCTTAAAAAATTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGGAGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCC CCGTATAGAAGAGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATC ACAGAAAAGATGAAAAGAGAAAAGAAAAATGTAGGCATCATAGCCATTCAGCAGAAGGGGGGAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCG GAAACGACATCGAGAAGAACAGGATAAAGCTCGCCGGGAATGGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGGGACCGC TTGGAGCAGTTAGAAAGGAAGCGGGAGCGGGAGCGCAAGATGCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCGGGCGGAGGAGC GGCGCAAGGAGCGGGAGGCCCGCAGGGAAGTGTCTGCACATCACCGAACGATGAGAGAGGACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAG CCCGCCTCGGCCGCCGCGGGAGCGGTTCGAGTTGGGAGACGGCCGGAAGCCAGGTGAGGCCAGGCCGGCGCCTGCGCAGAAGCCAGCACAGTTAAAAGAA GAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACAGGACATCAGCGACAGCGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGAATCAGGCT CAGGTTCTGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGGAGCACCAGTGAAGAATCAGAGGAGGAGGAGGAGGAAGAGGAAGAGGAGGA GGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGCCGAAGAAGTAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAAATGAAAAC CACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTCCGGGGAGAGTGAAGAAGCAGAGGAAGAAGTGGGTGAGGGAACGCCGCAGAGCAGCGCCC TGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGTTGCCCATCGAGCTCAAGCAGGAGCTGCCCAAGTACCTGCCGGCCCTGCAGGGCTGCCGGAG CGTCGAGGAGTTCCAGTGCCTGAACAGGATCGAGGAGGGCACCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAAAG CGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCCCTGAGGGAGATCAACACCATCCTCAAGGCCCAGCATCCCAACATTGTCACCGTTA GAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATCGTGATGAACTACGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCTT CCTGCCAGGGGAGGTGAAGACCCTGATGATCCAGCTGCTGCGGGGGGTGAAACACCTGCACGACAACTGGATCCTGCACCGTGACCTCAAGACGTCCAAC CTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTTTGGGCTGGCGCGGGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACCC AGTGGTACCGCGCCCCAGAGCTGCTGCTTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGTCAGTGGGCTGCATCTTCGGGGAGCTGCTGACTCA GAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCAACAAAGTGTTCAAGGAGCTGGGGACCCCCAGTGAGAAAATCTGGCCCGGCTACAGTGAG CTCCCAGTAGTCAAAAAGATGACCTTCAGCGAGCACCCCTACAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCATGA ACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATTTCCGCGAGACCCCCCTCCCCATCGACCCCTCCAT GTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGAAGCGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGAC GACGACCTGAAGGAGACGGGCTTCCACCTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTCAAGTTCTGAAGGTCAGAGTGGA CCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGVSGGVL TQMGDEKDSWKVKTLDEILQEKKRRKEQEEKAEI KRLKNSDDRDSKRDSLEEGELRDHCMEITIRNS PYRREDSMEDRGEEDDSLAIKPPQQMSRKEKVH HRKDEKRKEKCRHHSHSAEGGKHARVKEREHERR KRHREEQDKARREWERQKRREMAREHSRRERDR LEQLERKRERERKMREQQKEQREQKERERRAEE RRKEREARREVSAHHRTMREDYSDKVKASHWSRS PPRPPRERFELGDGRKPGEARPAPAQKPAQLKE EKMEERDLLSDLQDISDSERKTSSAESSSAESG SGSEEEEEEEEEEEEEGSTSEESEEEEEEEEEEE EETGSNSEEASEQSAEEVSEEEMSEDEERENEN HLLVVPESRFDRDSGESEEAEEEVGEGTPQSSA LTEGDYVPDSPALLPIELKQELPKYLPALQGCRS VEEFQCLNRIEEGTYGVVYRAKDKKTDEIVALK RLKMEKEKEGFPITSLREINTILKAQHPNIVTV REIVVGSNMDKIYIVMNYVEHDLKSLMETMKQPF LPGEVKTLMIQLLRGVKHLHDNWILHRDLKTSN LLLSHAGILKVGDFGLAREYGSPLKAYTPVVVT QWYRAPELLLGAKEYSTAVDMWSVGCIFGELLTQ KPLFPGNSEIDQINKVFKELGTPSEKIWPGYSE LPVVKKMTFSEHPYNNLRKRFGALLSDQGFDLM NKFLTYFPGRRISAEDGLKHEYFRETPLPIDPSM FPTWPAKSEQQRVKRGTSPRPPEGGLGYSQLGD DDLKETGFHLTTTNQGASAAGPGFSLKF*RSEW TPSW

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*G*AAVF* LKWVMKRTLGK*KL*MKFFRKRNEGRNKRRKQR *NA*KILMTGIPSGIPLRRGS*EITAWRSQ*GTP RIEEKTQWKTEEKKMILWPSNHPSKCLGKKKFI TEKMKREKKNVGIIAIQQKGGSMLE*KKESTNV GNDIEKNRIKLAGNGKDRREGKWQGSIPGEKGTA WSS*KGSGSGSARCGSSRRSSGSRRSASGGRRS GARSGRPAGKCLHITER*ERTTATK*KPATGVA ARLGRRGSGSSWETAGSQVRPGRRLRRSQHS*KK RKWKKGTCCPTYRTSATARGRPARPSPRQRNQA QVLRKKRRRRKRRRRKGAPVKNQRRRRRKRKRR RRRPAATLRRHQSSLPKK*VRKK*VKMKNEKMKT TSWLFQSHGSTEIPGRVKKQRKKWVRERRRAAP *QRATMCPTPLPCCPSSSSRSCPSTCRPCRAAG ASRSSSA*TGSRRAPMEWSTEQKTRKQMKLWL*S G*RWRRRRRASRSRP*GRSTPSSRPSIPTLSPL ERLWWAATWTRSTS**TTWSTTSRA*WRP*NSP SCQGR*RP**SSCCGG*NTCTTTGSCTVTSRRPT CC*ATPASSRWVILGWRGSTDPL*RPTPRSW*P SGTAPQSCCLVPRNTPRPWTCGQWAASSGSC*L RSLCSPGIRKSIRSTKCSRSWGPPVRKSGPATVS SQ*SKR*PSASTPTTTSASASGLCSQTRASTS* TSS*PTSPGGGSALRTASSMSISARPPSPSTPP CSPRGPPRASSSV*SGAPARGPLREAWATASWVT TT*RRRASTLPPRTRGPLPRAPASASSSEGQSG PRHG

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRGERRCFN SNG**KGLLESENFR*NSSGKETKEGTRGESRD KTLKKF**PGFQAGFP*GGGAERSLHGDHNKEL PV*KRRLNGRQRRRR*FFGHQTTPANVSERKSSS QKR*KEKRKM*AS*PFSRRGEAC*SERKRARTS ETTSRRTG*SSPGMGKTEEKGNGKGAFQERKGP LGAVRKEAGAGAQDAGAAEGAAGAEGARAAGGGA AQGAGGPQGSVCTSPNDERGLQRQSESQPLESQ PASAAAGAVRVGRRPEAR*GQAGACAEASTVKR RENGRKGPAVRLTGHQRQREEDQLGRVLVSGIRL RF*GRRGGGGRGGGGREHQ*RIRGGGGGRGRGG GGDRQQL*GGIRAVCRRSK*GRNE*R*RTRK*K PPLGCSRVTVRPRFRGE*RSRGRSG*GNAAEQRP DRGRLCARLPCPVAHRAQAGAAQVPAGPAGLPE RRGVPVPEQDRGGHLWSGLQSKRQENR*NCGSK AAEDGEGEGGLPDHVPEGDQHHPQGPASQHCHR* RDCGGQQHGQDLHRDELRGARPQEPDGDHETAL PARGGEDPDDPAAAGGETPARQLDPAP*PQDVQ PAAEPRRHPQGG*FWAGAGVRIPSEGLHPGRGDP VVPRPRAAAWCQGILHGRGHVVSGLHLRGAADS EASVPREFGNRSDQQSVQGAGDPQ*ENLARLQ* APSSQKDDLQRAPLQQPPQALRGSALRPGLRPHE QVPDLLPREEDQR*GRPQA*VFPRDPPPHRPLH VPHVARQERAAACEAGHQPEAP*GRPGLQPAG* RRPEGDGLPPYHHEPGGLCRGPRLQPQVLKVRVD PVMG

Sequence NM_033486

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGGTGAGCGGCGGTGTTTTAA CTCAAATGGGTGATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGAT AAAACGCTTAAAAAATTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGGAGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCC CCGTATAGAAGAGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATC ACAGAAAAGATGAAAAGAGAAAAGAAAAATGTAGGCATCATAGCCATTCAGCAGAAGGGGGGAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCG GAAACGACATCGAGAAGAACAGGATAAAGCTCGCCGGGAATGGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGGGACCGC TTGGAGCAGTTAGAAAGGAAGCGGGAGCGGGAGCGCAAGATGCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCGGGCGGAGGAGC GGCGCAAGGAGCGGGAGGCCCGCAGGGAAGTGTCTGCACATCACCGAACGATGAGAGAGGACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAG CCCGCCTCGGCCGCCGCGGGAGCGGTTCGAGTTGGGAGACGGCCGGAAGCCAGTAAAAGAAGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACAG GACATCAGCGACAGCGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGAATCAGGCTCAGGTTCTGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGG AGGAGGAAGGGAGCACCAGTGAAGAATCAGAGGAGGAGGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCA GTCTGCCGAAGAAGTAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGAT TCCGGGGAGAGTGAAGAAGCAGAGGAAGAAGTGGGTGAGGGAACGCCGCAGAGCAGCGCCCTGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGT TGCCCATCGAGCTCAAGCAGGAGCTGCCCAAGTACCTGCCGGCCCTGCAGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGAGGAGGG CACCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAAAGCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATC ACGTCCCTGAGGGAGATCAACACCATCCTCAAGGCCCAGCATCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACA TCGTGATGAACTACGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAGGGGAGGTGAAGACCCTGATGATCCAGCTGCT GCGGGGGGTGAAACACCTGCACGACAACTGGATCCTGCACCGTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGAT TTTGGGCTGGCGCGGGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTGCTGCTTGGTGCCAAGG AATACTCCACGGCCGTGGACATGTGGTCAGTGGGCTGCATCTTCGGGGAGCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGAT CAACAAAGTGTTCAAGGAGCTGGGGACCCCCAGTGAGAAAATCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAAAAGATGACCTTCAGCGAGCACCCC TACAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCATGAACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCG CTGAGGACGGCCTCAAGCATGAGTATTTCCGCGAGACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGT GAAGCGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCTGAAGGAGACGGGCTTCCACCTTACCACCACG AACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTCAAGTTCTGAAGGTCAGAGTGGACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGVSGGVL TQMGDEKDSWKVKTLDEILQEKKRRKEQEEKAEI KRLKNSDDRDSKRDSLEEGELRDHCMEITIRNS PYRREDSMEDRGEEDDSLAIKPPQQMSRKEKVH HRKDEKRKEKCRHHSHSAEGGKHARVKEREHERR KRHREEQDKARREWERQKRREMAREHSRRERDR LEQLERKRERERKMREQQKEQREQKERERRAEE RRKEREARREVSAHHRTMREDYSDKVKASHWSRS PPRPPRERFELGDGRKPVKEEKMEERDLLSDLQ DISDSERKTSSAESSSAESGSGSEEEEEEEEEE EEEGSTSEESEEEEEEEEEEEEETGSNSEEASEQ SAEEVSEEEMSEDEERENENHLLVVPESRFDRD SGESEEAEEEVGEGTPQSSALTEGDYVPDSPAL LPIELKQELPKYLPALQGCRSVEEFQCLNRIEEG TYGVVYRAKDKKTDEIVALKRLKMEKEKEGFPI TSLREINTILKAQHPNIVTVREIVVGSNMDKIY IVMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLL RGVKHLHDNWILHRDLKTSNLLLSHAGILKVGD FGLAREYGSPLKAYTPVVVTQWYRAPELLLGAK EYSTAVDMWSVGCIFGELLTQKPLFPGNSEIDQI NKVFKELGTPSEKIWPGYSELPVVKKMTFSEHP YNNLRKRFGALLSDQGFDLMNKFLTYFPGRRIS AEDGLKHEYFRETPLPIDPSMFPTWPAKSEQQRV KRGTSPRPPEGGLGYSQLGDDDLKETGFHLTTT NQGASAAGPGFSLKF*RSEWTPSW

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*G*AAVF* LKWVMKRTLGK*KL*MKFFRKRNEGRNKRRKQR *NA*KILMTGIPSGIPLRRGS*EITAWRSQ*GTP RIEEKTQWKTEEKKMILWPSNHPSKCLGKKKFI TEKMKREKKNVGIIAIQQKGGSMLE*KKESTNV GNDIEKNRIKLAGNGKDRREGKWQGSIPGEKGTA WSS*KGSGSGSARCGSSRRSSGSRRSASGGRRS GARSGRPAGKCLHITER*ERTTATK*KPATGVA ARLGRRGSGSSWETAGSQ*KKRKWKKGTCCPTYR TSATARGRPARPSPRQRNQAQVLRKKRRRRKRR RRKGAPVKNQRRRRRKRKRRRRRPAATLRRHQS SLPKK*VRKK*VKMKNEKMKTTSWLFQSHGSTEI PGRVKKQRKKWVRERRRAAP*QRATMCPTPLPC CPSSSSRSCPSTCRPCRAAGASRSSSA*TGSRR APMEWSTEQKTRKQMKLWL*SG*RWRRRRRASRS RP*GRSTPSSRPSIPTLSPLERLWWAATWTRST S**TTWSTTSRA*WRP*NSPSCQGR*RP**SSC CGG*NTCTTTGSCTVTSRRPTCC*ATPASSRWVI LGWRGSTDPL*RPTPRSW*PSGTAPQSCCLVPR NTPRPWTCGQWAASSGSC*LRSLCSPGIRKSIR STKCSRSWGPPVRKSGPATVSSQ*SKR*PSASTP TTTSASASGLCSQTRASTS*TSS*PTSPGGGSA LRTASSMSISARPPSPSTPPCSPRGPPRASSSV *SGAPARGPLREAWATASWVTTT*RRRASTLPPR TRGPLPRAPASASSSEGQSGPRHG

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRGERRCFN SNG**KGLLESENFR*NSSGKETKEGTRGESRD KTLKKF**PGFQAGFP*GGGAERSLHGDHNKEL PV*KRRLNGRQRRRR*FFGHQTTPANVSERKSSS QKR*KEKRKM*AS*PFSRRGEAC*SERKRARTS ETTSRRTG*SSPGMGKTEEKGNGKGAFQERKGP LGAVRKEAGAGAQDAGAAEGAAGAEGARAAGGGA AQGAGGPQGSVCTSPNDERGLQRQSESQPLESQ PASAAAGAVRVGRRPEASKRRENGRKGPAVRLT GHQRQREEDQLGRVLVSGIRLRF*GRRGGGGRGG GGREHQ*RIRGGGGGRGRGGGGDRQQL*GGIRA VCRRSK*GRNE*R*RTRK*KPPLGCSRVTVRPR FRGE*RSRGRSG*GNAAEQRPDRGRLCARLPCPV AHRAQAGAAQVPAGPAGLPERRGVPVPEQDRGG HLWSGLQSKRQENR*NCGSKAAEDGEGEGGLPD HVPEGDQHHPQGPASQHCHR*RDCGGQQHGQDLH RDELRGARPQEPDGDHETALPARGGEDPDDPAA AGGETPARQLDPAP*PQDVQPAAEPRRHPQGG* FWAGAGVRIPSEGLHPGRGDPVVPRPRAAAWCQG ILHGRGHVVSGLHLRGAADSEASVPREFGNRSD QQSVQGAGDPQ*ENLARLQ*APSSQKDDLQRAP LQQPPQALRGSALRPGLRPHEQVPDLLPREEDQR *GRPQA*VFPRDPPPHRPLHVPHVARQERAAAC EAGHQPEAP*GRPGLQPAG*RRPEGDGLPPYHH EPGGLCRGPRLQPQVLKVRVDPVMG

Sequence NM_033492

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGGTGAGCGGCGGTGTTTTAA CTCAAATGGGTGATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGAT AAAACGCTTAAAAAATTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGGAGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCC CCGTATAGAAGAGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATC ACAGAAAAGATGAAAAGAGAAAAGAAAAAAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCGGAAACGACATCGAGAAGAACAGGATAAAGCTCG CCGGGAATGGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGGGGGAATGATGGCGTGTGCCTCTTCAGGGACCGCTTGGAG CAGTTAGAAAGGAAGCGGGAGCGGGAGCGCAAGATGCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCGGGCGGAGGAGCGGCGCA AGGAGCGGGAGGCCCGCAGGGAAGTGTCTGCACATCACCGAACGATGAGAGAGGACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAGCCCGCC TCGGCCGCCGCGGGAGCGGTTCGAGTTGGGAGACGGCCGGAAGCCAGTAAAAGAAGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACAGGACATC AGCGACAGCGAGAGGAAGACCAGCTCGGCCGAGTCCTCGTCAGCGGAATCAGGCTCAGGTTCTGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGG AAGGGAGCACCAGTGAAGAATCAGAGGAGGAGGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGC CGAAGAAGTAAGTGAGGAAGAAATGAGTGAAGATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTCCGGG GAGAGTGAAGAAGCAGAGGAAGAAGTGGGTGAGGGAACGCCGCAGAGCAGCGCCCTGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGTTGCCCA TCGAGCTCAAGCAGGAGCTGCCCAAGTACCTGCCGGCCCTGCAGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGAGGAGGGCACCTA TGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAAAGCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCC CTGAGGGAGATCAACACCATCCTCAAGGCCCAGCATCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATCGTGA TGAACTACGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAGGGGAGGTGAAGACCCTGATGATCCAGCTGCTGCGGGG GGTGAAACACCTGCACGACAACTGGATCCTGCACCGTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTTTGGG CTGGCGCGGGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTGCTGCTTGGTGCCAAGGAATACT CCACGGCCGTGGACATGTGGTCAGTGGGCTGCATCTTCGGGGAGCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCAACAA AGTGTTCAAGGAGCTGGGGACCCCCAGTGAGAAAATCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAAAAGATGACCTTCAGCGAGCACCCCTACAAC AACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCATGAACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGG ACGGCCTCAAGCATGAGTATTTCCGCGAGACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGAAGCG GGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCTGAAGGAGACGGGCTTCCACCTTACCACCACGAACCAG GGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTCAAGTTCTGAAGGTCAGAGTGGACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGVSGGVL TQMGDEKDSWKVKTLDEILQEKKRRKEQEEKAEI KRLKNSDDRDSKRDSLEEGELRDHCMEITIRNS PYRREDSMEDRGEEDDSLAIKPPQQMSRKEKVH HRKDEKRKEKKHARVKEREHERRKRHREEQDKAR REWERQKRREMAREHSRRERGNDGVCLFRDRLE QLERKRERERKMREQQKEQREQKERERRAEERR KEREARREVSAHHRTMREDYSDKVKASHWSRSPP RPPRERFELGDGRKPVKEEKMEERDLLSDLQDI SDSERKTSSAESSSAESGSGSEEEEEEEEEEEE EGSTSEESEEEEEEEEEEEEETGSNSEEASEQSA EEVSEEEMSEDEERENENHLLVVPESRFDRDSG ESEEAEEEVGEGTPQSSALTEGDYVPDSPALLP IELKQELPKYLPALQGCRSVEEFQCLNRIEEGTY GVVYRAKDKKTDEIVALKRLKMEKEKEGFPITS LREINTILKAQHPNIVTVREIVVGSNMDKIYIV MNYVEHDLKSLMETMKQPFLPGEVKTLMIQLLRG VKHLHDNWILHRDLKTSNLLLSHAGILKVGDFG LAREYGSPLKAYTPVVVTQWYRAPELLLGAKEY STAVDMWSVGCIFGELLTQKPLFPGNSEIDQINK VFKELGTPSEKIWPGYSELPVVKKMTFSEHPYN NLRKRFGALLSDQGFDLMNKFLTYFPGRRISAE DGLKHEYFRETPLPIDPSMFPTWPAKSEQQRVKR GTSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQ GASAAGPGFSLKF*RSEWTPSW

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*G*AAVF* LKWVMKRTLGK*KL*MKFFRKRNEGRNKRRKQR *NA*KILMTGIPSGIPLRRGS*EITAWRSQ*GTP RIEEKTQWKTEEKKMILWPSNHPSKCLGKKKFI TEKMKREKKKSMLE*KKESTNVGNDIEKNRIKL AGNGKDRREGKWQGSIPGEKGGMMACASSGTAWS S*KGSGSGSARCGSSRRSSGSRRSASGGRRSGA RSGRPAGKCLHITER*ERTTATK*KPATGVAAR LGRRGSGSSWETAGSQ*KKRKWKKGTCCPTYRTS ATARGRPARPSPRQRNQAQVLRKKRRRRKRRRR KGAPVKNQRRRRRKRKRRRRRPAATLRRHQSSL PKK*VRKK*VKMKNEKMKTTSWLFQSHGSTEIPG RVKKQRKKWVRERRRAAP*QRATMCPTPLPCCP SSSSRSCPSTCRPCRAAGASRSSSA*TGSRRAP MEWSTEQKTRKQMKLWL*SG*RWRRRRRASRSRP *GRSTPSSRPSIPTLSPLERLWWAATWTRSTS* *TTWSTTSRA*WRP*NSPSCQGR*RP**SSCCG G*NTCTTTGSCTVTSRRPTCC*ATPASSRWVILG WRGSTDPL*RPTPRSW*PSGTAPQSCCLVPRNT PRPWTCGQWAASSGSC*LRSLCSPGIRKSIRST KCSRSWGPPVRKSGPATVSSQ*SKR*PSASTPTT TSASASGLCSQTRASTS*TSS*PTSPGGGSALR TASSMSISARPPSPSTPPCSPRGPPRASSSV*S GAPARGPLREAWATASWVTTT*RRRASTLPPRTR GPLPRAPASASSSEGQSGPRHG

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRGERRCFN SNG**KGLLESENFR*NSSGKETKEGTRGESRD KTLKKF**PGFQAGFP*GGGAERSLHGDHNKEL PV*KRRLNGRQRRRR*FFGHQTTPANVSERKSSS QKR*KEKRKKAC*SERKRARTSETTSRRTG*SS PGMGKTEEKGNGKGAFQERKGE*WRVPLQGPLG AVRKEAGAGAQDAGAAEGAAGAEGARAAGGGAAQ GAGGPQGSVCTSPNDERGLQRQSESQPLESQPA SAAAGAVRVGRRPEASKRRENGRKGPAVRLTGH QRQREEDQLGRVLVSGIRLRF*GRRGGGGRGGGG REHQ*RIRGGGGGRGRGGGGDRQQL*GGIRAVC RRSK*GRNE*R*RTRK*KPPLGCSRVTVRPRFR GE*RSRGRSG*GNAAEQRPDRGRLCARLPCPVAH RAQAGAAQVPAGPAGLPERRGVPVPEQDRGGHL WSGLQSKRQENR*NCGSKAAEDGEGEGGLPDHV PEGDQHHPQGPASQHCHR*RDCGGQQHGQDLHRD ELRGARPQEPDGDHETALPARGGEDPDDPAAAG GETPARQLDPAP*PQDVQPAAEPRRHPQGG*FW AGAGVRIPSEGLHPGRGDPVVPRPRAAAWCQGIL HGRGHVVSGLHLRGAADSEASVPREFGNRSDQQ SVQGAGDPQ*ENLARLQ*APSSQKDDLQRAPLQ QPPQALRGSALRPGLRPHEQVPDLLPREEDQR*G RPQA*VFPRDPPPHRPLHVPHVARQERAAACEA GHQPEAP*GRPGLQPAG*RRPEGDGLPPYHHEP GGLCRGPRLQPQVLKVRVDPVMG

Sequence NM_033493

ACACAGGAAGTGAGGATACTTCTGGCGAGCGCCGGTTGCTGTTTCTTCTCAGGCTCAGGGACCGGCCGCGGCCCCGTAGGGGTGAGCGGCGGTGTTTTAA CTCAAATGGGTGATGAAAAGGACTCTTGGAAAGTGAAAACTTTAGATGAAATTCTTCAGGAAAAGAAACGAAGGAAGGAACAAGAGGAGAAAGCAGAGAT AAAACGCTTAAAAAATTCTGATGACCGGGATTCCAAGCGGGATTCCCTTGAGGAGGGGGAGCTGAGAGATCACTGCATGGAGATCACAATAAGGAACTCC CCGTATAGAAGAGAAGACTCAATGGAAGACAGAGGAGAAGAAGATGATTCTTTGGCCATCAAACCACCCCAGCAAATGTCTCGGAAAGAAAAAGTTCATC ACAGAAAAGATGAAAAGAGAAAAGAAAAAAAGCATGCTAGAGTGAAAGAAAGAGAGCACGAACGTCGGAAACGACATCGAGAAGAACAGGATAAAGCTCG CCGGGAATGGGAAAGACAGAAGAGAAGGGAAATGGCAAGGGAGCATTCCAGGAGAGAAAGGGACCGCTTGGAGCAGTTAGAAAGGAAGCGGGAGCGGGAG CGCAAGATGCGGGAGCAGCAGAAGGAGCAGCGGGAGCAGAAGGAGCGCGAGCGGCGGGCGGAGGAGCGGCGCAAGGAGCGGGAGGCCCGCAGGGAAGTGT CTGCACATCACCGAACGATGAGAGAGGACTACAGCGACAAAGTGAAAGCCAGCCACTGGAGTCGCAGCCCGCCTCGGCCGCCGCGGGAGCGGTTCGAGTT GGGAGACGGCCGGAAGCCAGTAAAAGAAGAGAAAATGGAAGAAAGGGACCTGCTGTCCGACTTACAGGACATCAGCGACAGCGAGAGGAAGACCAGCTCG GCCGAGTCCTCGTCAGCGGAATCAGGCTCAGGTTCTGAGGAAGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGGGAGCACCAGTGAAGAATCAGAGG AGGAGGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGACCGGCAGCAACTCTGAGGAGGCATCAGAGCAGTCTGCCGAAGAAGTAAGTGAGGAAGAAATGAG TGAAGATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTCCGGGGAGAGTGAAGAAGCAGAGGAAGAAGTG GGTGAGGGAACGCCGCAGAGCAGCGCCCTGACAGAGGGCGACTATGTGCCCGACTCCCCTGCCCTGTTGCCCATCGAGCTCAAGCAGGAGCTGCCCAAGT ACCTGCCGGCCCTGCAGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGAGGAGGGCACCTATGGAGTGGTCTACAGAGCAAAAGACAA GAAAACAGATGAAATTGTGGCTCTAAAGCGGCTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCCCTGAGGGAGATCAACACCATCCTCAAG GCCCAGCATCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATCGTGATGAACTACGTGGAGCACGACCTCAAGA GCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAGGGGAGGTGAAGACCCTGATGATCCAGCTGCTGCGGGGGGTGAAACACCTGCACGACAACTGGAT CCTGCACCGTGACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTTTGGGCTGGCGCGGGAGTACGGATCCCCTCTG AAGGCCTACACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTGCTGCTTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGTCAGTGG GCTGCATCTTCGGGGAGCTGCTGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCAACAAAGTGTTCAAGGAGCTGGGGACCCCCAG TGAGAAAATCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAAAAGATGACCTTCAGCGAGCACCCCTACAACAACCTCCGCAAGCGCTTCGGGGCTCTG CTCTCAGACCAGGGCTTCGACCTCATGAACAAGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATTTCCGCG AGACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGAAGCGGGGCACCAGCCCGAGGCCCCCTGAGGG AGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCTGAAGGAGACGGGCTTCCACCTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTC AGCCTCAAGTTCTGAAGGTCAGAGTGGACCCCGTCATGGGG

refseq_CDC2L2.F3 (forward primer)
refseq_CDC2L2.R3 (reverse primer)
Alternative donor

Frame 0

TQEVRILLASAGCCFFSGSGTGRGPVGVSGGVL TQMGDEKDSWKVKTLDEILQEKKRRKEQEEKAEI KRLKNSDDRDSKRDSLEEGELRDHCMEITIRNS PYRREDSMEDRGEEDDSLAIKPPQQMSRKEKVH HRKDEKRKEKKHARVKEREHERRKRHREEQDKAR REWERQKRREMAREHSRRERDRLEQLERKRERE RKMREQQKEQREQKERERRAEERRKEREARREV SAHHRTMREDYSDKVKASHWSRSPPRPPRERFEL GDGRKPVKEEKMEERDLLSDLQDISDSERKTSS AESSSAESGSGSEEEEEEEEEEEEEGSTSEESE EEEEEEEEEEEETGSNSEEASEQSAEEVSEEEMS EDEERENENHLLVVPESRFDRDSGESEEAEEEV GEGTPQSSALTEGDYVPDSPALLPIELKQELPK YLPALQGCRSVEEFQCLNRIEEGTYGVVYRAKDK KTDEIVALKRLKMEKEKEGFPITSLREINTILK AQHPNIVTVREIVVGSNMDKIYIVMNYVEHDLK SLMETMKQPFLPGEVKTLMIQLLRGVKHLHDNWI LHRDLKTSNLLLSHAGILKVGDFGLAREYGSPL KAYTPVVVTQWYRAPELLLGAKEYSTAVDMWSV GCIFGELLTQKPLFPGNSEIDQINKVFKELGTPS EKIWPGYSELPVVKKMTFSEHPYNNLRKRFGAL LSDQGFDLMNKFLTYFPGRRISAEDGLKHEYFR ETPLPIDPSMFPTWPAKSEQQRVKRGTSPRPPEG GLGYSQLGDDDLKETGFHLTTTNQGASAAGPGF SLKF*RSEWTPSW

Frame 1

HRK*GYFWRAPVAVSSQAQGPAAAP*G*AAVF* LKWVMKRTLGK*KL*MKFFRKRNEGRNKRRKQR *NA*KILMTGIPSGIPLRRGS*EITAWRSQ*GTP RIEEKTQWKTEEKKMILWPSNHPSKCLGKKKFI TEKMKREKKKSMLE*KKESTNVGNDIEKNRIKL AGNGKDRREGKWQGSIPGEKGTAWSS*KGSGSGS ARCGSSRRSSGSRRSASGGRRSGARSGRPAGKC LHITER*ERTTATK*KPATGVAARLGRRGSGSS WETAGSQ*KKRKWKKGTCCPTYRTSATARGRPAR PSPRQRNQAQVLRKKRRRRKRRRRKGAPVKNQR RRRRKRKRRRRRPAATLRRHQSSLPKK*VRKK* VKMKNEKMKTTSWLFQSHGSTEIPGRVKKQRKKW VRERRRAAP*QRATMCPTPLPCCPSSSSRSCPS TCRPCRAAGASRSSSA*TGSRRAPMEWSTEQKT RKQMKLWL*SG*RWRRRRRASRSRP*GRSTPSSR PSIPTLSPLERLWWAATWTRSTS**TTWSTTSR A*WRP*NSPSCQGR*RP**SSCCGG*NTCTTTG SCTVTSRRPTCC*ATPASSRWVILGWRGSTDPL* RPTPRSW*PSGTAPQSCCLVPRNTPRPWTCGQW AASSGSC*LRSLCSPGIRKSIRSTKCSRSWGPP VRKSGPATVSSQ*SKR*PSASTPTTTSASASGLC SQTRASTS*TSS*PTSPGGGSALRTASSMSISA RPPSPSTPPCSPRGPPRASSSV*SGAPARGPLR EAWATASWVTTT*RRRASTLPPRTRGPLPRAPAS ASSSEGQSGPRHG

Frame 2

TGSEDTSGERRLLFLLRLRDRPRPRRGERRCFN SNG**KGLLESENFR*NSSGKETKEGTRGESRD KTLKKF**PGFQAGFP*GGGAERSLHGDHNKEL PV*KRRLNGRQRRRR*FFGHQTTPANVSERKSSS QKR*KEKRKKAC*SERKRARTSETTSRRTG*SS PGMGKTEEKGNGKGAFQERKGPLGAVRKEAGAG AQDAGAAEGAAGAEGARAAGGGAAQGAGGPQGSV CTSPNDERGLQRQSESQPLESQPASAAAGAVRV GRRPEASKRRENGRKGPAVRLTGHQRQREEDQL GRVLVSGIRLRF*GRRGGGGRGGGGREHQ*RIRG GGGGRGRGGGGDRQQL*GGIRAVCRRSK*GRNE *R*RTRK*KPPLGCSRVTVRPRFRGE*RSRGRS G*GNAAEQRPDRGRLCARLPCPVAHRAQAGAAQV PAGPAGLPERRGVPVPEQDRGGHLWSGLQSKRQ ENR*NCGSKAAEDGEGEGGLPDHVPEGDQHHPQ GPASQHCHR*RDCGGQQHGQDLHRDELRGARPQE PDGDHETALPARGGEDPDDPAAAGGETPARQLD PAP*PQDVQPAAEPRRHPQGG*FWAGAGVRIPS EGLHPGRGDPVVPRPRAAAWCQGILHGRGHVVSG LHLRGAADSEASVPREFGNRSDQQSVQGAGDPQ *ENLARLQ*APSSQKDDLQRAPLQQPPQALRGS ALRPGLRPHEQVPDLLPREEDQR*GRPQA*VFPR DPPPHRPLHVPHVARQERAAACEAGHQPEAP*G RPGLQPAG*RRPEGDGLPPYHHEPGGLCRGPRL QPQVLKVRVDPVMG