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Sequences

Sequence NM_006375

GTGTGTGAGAGTCCAGCCTTGGGCCAGAGTGCCGGGTCTAGGCCTGGAAGCGCGAGGCGGGCATTTCGGAAGAGAGTGGCGCGGCGTGGGGCGCCGCGAG GGCACTACGAGCCCAGGAACTGCCGCGCCGGCCCGATTTTCCGCGGCTGTATAAGGACTAGCGCTTCGGTAGCCGGGGAGCTGGAGGGAACCTGATTTGA GATCAATTCTTGGAGAACACAAAGAAGTGTGATGGATTGAACCAGCTGCCCTTTGACCCTGCTAACAACAACGAGCCCCTGCAGTTTGGTAGTGCCAGTG GCCCTCTGGTCACAGAAGGCCTCATTGAGAATGGAGGGGAATCAAGCAAGAAAAGAAAGAGAACAAATACTCCTTCAGCCGATAACAGTAGAACTCTGAA CGTGGATTCCACTGCAATGACACTACCTATGTCTGATCCAACTGCATGGGCCACAGCAATGAATAATCTTGGAATGGCACCGCTGGGAATTGCCGGACAA CCAATTTTACCTGACTTTGATCCTGCTCTTGGAATGATGACTGGAATTCCACCAATAACTCCAATGATGCCTGGTTTGGGAATAGTACCTCCACCAATTC CTCCAGATATGCCAGTAGTAAAAGAGATCATACACTGTAAAAGCTGCACGCTCTTCCCTCCAAATCCAAATCTCCCACCTCCTGCAACCCGAGAAAGACC ACCAGGATGCAAAACAGTATTTGTGGGTGGTCTGCCTGAAAATGGGACAGAGCAAATCATTGTGGAAGTTTTCGAGCAGTGTGGAGAGATCATTGCCATT CGCAAGAGCAAGAAGAACTTCTGCCACATTCGCTTTGCTGAGGAGTACATGGTGGACAAAGCCCTGTATCTGTCTGGTTACCGCATTCGCCTGGGCTCTA GTACTGACAAGAAGGACACAGGCAGACTCCACGTTGATTTCGCACAGGCTCGAGATGACCTGTATGAGTGGGAGTGTAAACAGCGTATGCTAGCCAGAGA GGAGCGCCATCGTAGAAGAATGGAAGAAGAAAGATTGCGTCCACCATCTCCACCCCCAGTGGTCCACTATTCAGATCATGAATGCAGCATTGTTGCTGAA AAATTAAAAGATGATTCCAAATTCTCAGAAGCTGTACAGACCTTGCTTACCTGGATAGAGCGAGGAGAGGTCAACCGTCGTAGCGCCAATAACTTCTACT CCATGATCCAGTCGGCCAACAGCCATGTCCGCCGCCTGGTGAACGAGAAAGCTGCCCATGAGAAAGATATGGAAGAAGCAAAGGAGAAGTTCAAGCAGGC CCTTTCTGGAATTCTCATTCAATTTGAGCAGATAGTGGCTGTGTACCATTCCGCCTCCAAGCAGAAGGCATGGGACCACTTCACAAAAGCCCAGCGGAAG AACATCAGCGTGTGGTGCAAACAAGCTGAGGAAATTCGCAACATTCATAATGATGAATTAATGGGAATCAGGCGAGAAGAAGAAATGGAAATGTCTGATG ATGAAATAGAAGAAATGACAGAAACAAAAGAAACTGAGGAATCAGCCTTAGTATCACAGGCAGAAGCTCTGAAGGAAGAAAATGACAGCCTCCGTTGGCA GCTCGATGCCTACCGGAATGAAGTAGAACTGCTCAAGCAAGAACAAGGCAAAGTCCACAGAGAAGATGACCCTAACAAAGAACAGCAGCTGAAACTCCTG CAACAAGCCCTGCAAGGAATGCAACAGCATCTACTCAAAGTCCAAGAGGAATACAAAAAGAAAGAAGCTGAACTTGAAAAACTCAAAGATGACAAGTTAC AGGTGGAAAAAATGTTGGAAAATCTTAAAGAAAAGGAAAGCTGTGCTTCTAGGCTGTGTGCCTCAAACCAGGATAGCGAATACCCTCTTGAGAAGACCAT GAACAGCAGTCCTATCAAATCTGAACGTGAAGCACTGCTAGTGGGGATTATCTCCACATTCCTTCATGTTCACCCATTTGGAGCAAGCATTGAATACATC TGTTCCTACTTGCACCGTCTTGATAATAAGATCTGCACCAGCGATGTGGAGTGTCTCATGGGTAGACTCCAGCATACCTTCAAGCAGGAAATGACTGGAG TTGGAGCCAGCCTGGAAAAGAGATGGAAATTCTGTGGCTTCGAGGGCTTGAAGCTGACCTAAATCTCTTTGCCTAACAACTTGGGATCCTGAAGATAAAT ATGTGTTGGACAAGCATAGAAAGTGATTTATATTTTTAATGGTTTTCAAGTGGAAGTTCCTTTGAATTTGTCAGTTCATTCCTGGAAAATCTTTTGAGTT AAAATAAGGATCCTAGGACAGCACCTCGAACTACAGGCCCTAAAGAGAAATTGCCTCAAACCACAAGTGCTGTAACTTCCTCCCCTTTCTGTCAATTGGT TGTCTTTAAATATTGCAAAAGTCCTGATGCTAAACAGTATTTGGAGTGTTTTCAGTGTCTGTACTACTGTTGTAGACCTTGGTATTTTTTTAAACACTGT TAACTGAAATGTTTTGATGATTTGTATGTGATTTGTGTTTCTAAACTTCTCTTTACATTAATGTTGTTACTGGTGAAAGGCATGAGAGCAGCACTAAGTC CTCTGTGTAACTGCCATTGTCTTTCCAATCCCCAGTAGACCAGTAAATAAATAACACATCAGTGTCTTCTAGAAGGTGCCTGACCAGGTTCACCTTTTAA ACGACAAAGCATGGTTTGTGGCTTTTTGCAAAATTACTATGAACCAAAAGTTGACAAATGTTCCAAAGTTATTTTCTCTAACATATCACATTAAAGATCT GTTTCAGAATTGTAAAAAGTACATCTAGATGTGTTTACAGAAAGCAAGTATCCAGTATGACTGGCATGTGTTCATGCTATTCAGAATCACTTGTAAATAG TCTGCTTTTAAAGGAGGGCATGTTCAGTTTTCTGTGAATTAAAATATGCTCATGTGTGGGCACACACGCACAAACACACACACGCACGCACACAGTGGCA GAAGGGATTTATATTAATATTCTTTCCCCTCTGGCCTTCTTACAGTCTGTTGGTCCCTTTGCTTCTGTTGTCAGTGTGTTGAATTGCAAACCGAGTACTG CTGTAAATACTATGTTTACTTCATGCTGAATGTTTGCAAAGACTTGATATAAGTATTAATAGTAATGAATCAATGAATAAATAATGAGCTAGGGTTTGTG AGGCTTTCTACAAATAGGTCAGCTCCACCTGGAGTGCGAATTGCCAGAGACACCTTGGTAGTGCCCATCGGCAAATCGCAATGGCAGCATGTGAGTGGAC CATTCAGAAACTTCTGCTTGGTGGAAAGTAAACAGAGAGGATGGAGGTTTGGGGCGAATGTCCTGAGGCAGAGATGGTCTTTATTGTGTGTGGTGGTGGT TGTGGTATTTATAATAATGCAAGCATACCCTCCCTTGAGTCTCAATTGAAGATAAAAGAATGTACTGAGCAAGCAAAGCCAATGGAGAGTATTTCACAAA AATACTTTGTAAATGAGATGCCAGTAGTGTTCAAAGTTGTATTTTTAAAAGATAAATATTCCTTTTTATACCTCAGTTTTGTGTCCTGTTTTTAATGACT TACGCTCTAAGTAATCCATTAGTAGTTATCTCAGTTCCTTCCTTTGGGTTACTAGAATGTTGGAAAAAGATGCCAAGTCTGTCTTGACAACTGGAAACAG GGTTCCACAGCAGCCCATTCGTGCTGAAAACTGGCTTCCCCCTGAAGCACCCTGCTGTGGCACCAGCAGGAAGCTCAGGTTAATTTTACACTAGCTTGCT CACTGATGCATCTCTCATCAATGCTACGGAAGGCTTTGATTCATCAGTCTCGGGCTCTTGGAATACCTAATTTTAATAATATCTATGAAATCAAGGGAAA CTTTCCATTTACAGTTATTTCTTGTTTAAATAAACTAAATTAATTTTTAGGGGAGAGCAGTAGGAAAAAGAGCTAATGCATGCGGGGTTTAATACCTAGG TGATGGGTTGAGGTGCAGCAAAACCACCATGGCACACGTTCACCTATGTAACAAACCTGCACATCCTGCACATGTACCCCGGAACTTACTTAAAA

refseq_COVA1.F2 (forward primer)
refseq_COVA1.R2 (reverse primer)
Single exon skipping

Frame 0

VCESPALGQSAGSRPGSARRAFRKRVARRGAPR GHYEPRNCRAGPIFRGCIRTSASVAGELEGT*FE INSWRTQRSVMD*TSCPLTLLTTTSPCSLVVPV ALWSQKASLRMEGNQARKEREQILLQPITVEL* TWIPLQ*HYLCLIQLHGPQQ*IILEWHRWELPDN QFYLTLILLLE**LEFHQ*LQ*CLVWE*YLHQF LQICQ**KRSYTVKAARSSLQIQISHLLQPEKD HQDAKQYLWVVCLKMGQSKSLWKFSSSVERSLPF ARARRTSATFALLRSTWWTKPCICLVTAFAWAL VLTRRTQADSTLISHRLEMTCMSGSVNSVC*PE RSAIVEEWKKKDCVHHLHPQWSTIQIMNAALLLK N*KMIPNSQKLYRPCLPG*SEERSTVVAPITST P*SSRPTAMSAAW*TRKLPMRKIWKKQRRSSSR PFLEFSFNLSR*WLCTIPPPSRRHGTTSQKPSGR TSACGANKLRKFATFIMMN*WESGEKKKWKCLM MK*KK*QKQKKLRNQP*YHRQKL*RKKMTASVG SSMPTGMK*NCSSKNKAKSTEKMTLTKNSS*NSC NKPCKECNSIYSKSKRNTKRKKLNLKNSKMTSY RWKKCWKILKKRKAVLLGCVPQTRIANTLLRRP *TAVLSNLNVKHC*WGLSPHSFMFTHLEQALNTS VPTCTVLIIRSAPAMWSVSWVDSSIPSSRK*LE LEPAWKRDGNSVASRA*S*PKSLCLTTWDPEDK YVLDKHRK*FIFLMVFKWKFL*ICQFIPGKSFEL K*GS*DSTSNYRP*REIASNHKCCNFLPFLSIG CL*ILQKS*C*TVFGVFSVSVLLL*TLVFF*TL LTEMF**FVCDLCF*TSLYINVVTGERHESSTKS SV*LPLSFQSPVDQ*INNTSVSSRRCLTRFTF* TTKHGLWLFAKLL*TKS*QMFQSYFL*HITLKI CFRIVKSTSRCVYRKQVSSMTGMCSCYSESLVNS LLLKEGMFSFL*IKICSCVGTHAQTHTRTHTVA EGIYINILSPLAFLQSVGPFASVVSVLNCKPST AVNTMFTSC*MFAKT*YKY****INE*IMS*GL* GFLQIGQLHLECELPETPW*CPSANRNGSM*VD HSETSAWWKVNREDGGLGRMS*GRDGLYCVWWW LWYL**CKHTLP*VSIEDKRMY*ASKANGEYFTK ILCK*DASSVQSCIFKR*IFLFIPQFCVLFLMT YALSNPLVVISVPSFGLLECWKKMPSLS*QLET GFHSSPFVLKTGFPLKHPAVAPAGSSG*FYTSLL TDASLINATEGFDSSVSGSWNT*F**YL*NQGK LSIYSYFLFK*TKLIFRGEQ*EKELMHAGFNT* VMG*GAAKPPWHTFTYVTNLHILHMYPGTYLK

Frame 1

CVRVQPWARVPGLGLEARGGHFGREWRGVGRRE GTTSPGTAAPARFSAAV*GLALR*PGSWREPDL RSILGEHKEV*WIEPAAL*PC*QQRAPAVW*CQW PSGHRRPH*EWRGIKQEKKENKYSFSR*Q*NSE RGFHCNDTTYV*SNCMGHSNE*SWNGTAGNCRT TNFT*L*SCSWNDDWNSTNNSNDAWFGNSTSTNS SRYASSKRDHTL*KLHALPSKSKSPTSCNPRKT TRMQNSICGWSA*KWDRANHCGSFRAVWRDHCH SQEQEELLPHSLC*GVHGGQSPVSVWLPHSPGL* Y*QEGHRQTPR*FRTGSR*PV*VGV*TAYASQR GAPS*KNGRRKIASTISTPSGPLFRS*MQHCC* KIKR*FQILRSCTDLAYLDRARRGQPS*RQ*LLL HDPVGQQPCPPPGERESCP*ERYGRSKGEVQAG PFWNSHSI*ADSGCVPFRLQAEGMGPLHKSPAE EHQRVVQTS*GNSQHS***INGNQARRRNGNV** *NRRNDRNKRN*GISLSITGRSSEGRK*QPPLA ARCLPE*SRTAQARTRQSPQRR*P*QRTAAETP ATSPARNATASTQSPRGIQKERS*T*KTQR*QVT GGKNVGKS*RKGKLCF*AVCLKPG*RIPS*EDH EQQSYQI*T*STASGDYLHIPSCSPIWSKH*IH LFLLAPS***DLHQRCGVSHG*TPAYLQAGNDWS WSQPGKEMEILWLRGLEADLNLFA*QLGILKIN MCWTSIESDLYF*WFSSGSSFEFVSSFLENLLS *NKDPRTAPRTTGPKEKLPQTTSAVTSSPFCQLV VFKYCKSPDAKQYLECFQCLYYCCRPWYFFKHC *LKCFDDLYVICVSKLLFTLMLLLVKGMRAALS PLCNCHCLSNPQ*TSK*ITHQCLLEGA*PGSPFK RQSMVCGFLQNYYEPKVDKCSKVIFSNISH*RS VSEL*KVHLDVFTESKYPV*LACVHAIQNHL*I VCF*RRACSVFCELKYAHVWAHTHKHTHARTQWQ KGFILIFFPLWPSYSLLVPLLLLSVC*IANRVL L*ILCLLHAECLQRLDISINSNESMNK**ARVC EAFYK*VSSTWSANCQRHLGSAHRQIAMAACEWT IQKLLLGGK*TERMEVWGECPEAEMVFIVCGGG CGIYNNASIPSLESQLKIKECTEQAKPMESISQ KYFVNEMPVVFKVVFLKDKYSFLYLSFVSCF**L TL*VIH**LSQFLPLGY*NVGKRCQVCLDNWKQ GSTAAHSC*KLASP*STLLWHQQEAQVNFTLAC SLMHLSSMLRKALIHQSRALGIPNFNNIYEIKGN FPFTVISCLNKLN*FLGESSRKKS*CMRGLIPR *WVEVQQNHHGTRSPM*QTCTSCTCTPELT*

Frame 2

V*ESSLGPECRV*AWKREAGISEESGAAWGAAR ALRAQELPRRPDFPRLYKD*RFGSRGAGGNLI* DQFLENTKKCDGLNQLPFDPANNNEPLQFGSAS GPLVTEGLIENGGESSKKRKRTNTPSADNSRTLN VDSTAMTLPMSDPTAWATAMNNLGMAPLGIAGQ PILPDFDPALGMMTGIPPITPMMPGLGIVPPPI PPDMPVVKEIIHCKSCTLFPPNPNLPPPATRERP PGCKTVFVGGLPENGTEQIIVEVFEQCGEIIAI RKSKKNFCHIRFAEEYMVDKALYLSGYRIRLGS STDKKDTGRLHVDFAQARDDLYEWECKQRMLARE ERHRRRMEEERLRPPSPPPVVHYSDHECSIVAE KLKDDSKFSEAVQTLLTWIERGEVNRRSANNFY SMIQSANSHVRRLVNEKAAHEKDMEEAKEKFKQA LSGILIQFEQIVAVYHSASKQKAWDHFTKAQRK NISVWCKQAEEIRNIHNDELMGIRREEEMEMSD DEIEEMTETKETEESALVSQAEALKEENDSLRWQ LDAYRNEVELLKQEQGKVHREDDPNKEQQLKLL QQALQGMQQHLLKVQEEYKKKEAELEKLKDDKL QVEKMLENLKEKESCASRLCASNQDSEYPLEKTM NSSPIKSEREALLVGIISTFLHVHPFGASIEYI CSYLHRLDNKICTSDVECLMGRLQHTFKQEMTG VGASLEKRWKFCGFEGLKLT*ISLPNNLGS*R*I CVGQA*KVIYIFNGFQVEVPLNLSVHSWKIF*V KIRILGQHLELQALKRNCLKPQVL*LPPLSVNW LSLNIAKVLMLNSIWSVFSVCTTVVDLGIFLNTV N*NVLMICM*FVFLNFSLH*CCYW*KA*EQH*V LCVTAIVFPIPSRPVNK*HISVF*KVPDQVHLL NDKAWFVAFCKITMNQKLTNVPKLFSLTYHIKDL FQNCKKYI*MCLQKASIQYDWHVFMLFRITCK* SAFKGGHVQFSVN*NMLMCGHTRTNTHTHAHSG RRDLY*YSFPSGLLTVCWSLCFCCQCVELQTEYC CKYYVYFMLNVCKDLI*VLIVMNQ*INNELGFV RLSTNRSAPPGVRIARDTLVVPIGKSQWQHVSG PFRNFCLVESKQRGWRFGANVLRQRWSLLCVVVV VVFIIMQAYPPLSLN*R*KNVLSKQSQWRVFHK NTL*MRCQ*CSKLYF*KINIPFYTSVLCPVFND LRSK*SISSYLSSFLWVTRMLEKDAKSVLTTGNR VPQQPIRAENWLPPEAPCCGTSRKLRLILH*LA H*CISHQCYGRL*FISLGLLEYLILIISMKSRE TFHLQLFLV*IN*INF*GRAVGKRANACGV*YLG DGLRCSKTTMAHVHLCNKPAHPAHVPRNLLK

Sequence NM_182314

GTGTGTGAGAGTCCAGCCTTGGGCCAGAGTGCCGGGTCTAGGCCTGGAAGCGCGAGGCGGGCATTTCGGAAGAGAGTGGCGCGGCGTGGGGCGCCGCGAG GGCACTACGAGCCCAGGAACTGCCGCGCCGGCCCGATTTTCCGCGGCTGTATAAGGACTAGCGCTTCGGTAGCCGGGGAGCTGGAGGGAACCTGATTTGA GATCAATTCTTGGAGAACACAAAGAAGTGTGATGGATTGAACCAGCTGCCCTTTGACCCTGCTAACAACAACGAGCCCCTGCAGTTTGGTAGTGCCAGTG GCCCTCTGGTCACAGAAGGCCTCATTGAGAATGGAGGGGAATCAAGCAAGAAAAGAAAGAGAACAAATACTCCTTCAGAAATGATGACAGAGCTAAGTTC ACAGTTGAGGACCACACAATGCAAAGAGATTTTAGATGGCTGTGGGTCTACGAAATAGGCTATGCAGCCGATAACAGTAGAACTCTGAACGTGGATTCCA CTGCAATGACACTACCTATGTCTGATCCAACTGCATGGGCCACAGCAATGAATAATCTTGGAATGGCACCGCTGGGAATTGCCGGACAACCAATTTTACC TGACTTTGATCCTGCTCTTGGAATGATGACTGGAATTCCACCAATAACTCCAATGATGCCTGGTTTGGGAATAGTACCTCCACCAATTCCTCCAGATATG CCAGTAGTAAAAGAGATCATACACTGTAAAAGCTGCACGCTCTTCCCTCCAAATCCAAATCTCCCACCTCCTGCAACCCGAGAAAGACCACCAGGATGCA AAACAGTATTTGTGGGTGGTCTGCCTGAAAATGGGACAGAGCAAATCATTGTGGAAGTTTTCGAGCAGTGTGGAGAGATCATTGCCATTCGCAAGAGCAA GAAGAACTTCTGCCACATTCGCTTTGCTGAGGAGTACATGGTGGACAAAGCCCTGTATCTGTCTGGTTACCGCATTCGCCTGGGCTCTAGTACTGACAAG AAGGACACAGGCAGACTCCACGTTGATTTCGCACAGGCTCGAGATGACCTGTATGAGTGGGAGTGTAAACAGCGTATGCTAGCCAGAGAGGAGCGCCATC GTAGAAGAATGGAAGAAGAAAGATTGCGTCCACCATCTCCACCCCCAGTGGTCCACTATTCAGATCATGAATGCAGCATTGTTGCTGAAAAATTAAAAGA TGATTCCAAATTCTCAGAAGCTGTACAGACCTTGCTTACCTGGATAGAGCGAGGAGAGGTCAACCGTCGTAGCGCCAATAACTTCTACTCCATGATCCAG TCGGCCAACAGCCATGTCCGCCGCCTGGTGAACGAGAAAGCTGCCCATGAGAAAGATATGGAAGAAGCAAAGGAGAAGTTCAAGCAGGCCCTTTCTGGAA TTCTCATTCAATTTGAGCAGATAGTGGCTGTGTACCATTCCGCCTCCAAGCAGAAGGCATGGGACCACTTCACAAAAGCCCAGCGGAAGAACATCAGCGT GTGGTGCAAACAAGCTGAGGAAATTCGCAACATTCATAATGATGAATTAATGGGAATCAGGCGAGAAGAAGAAATGGAAATGTCTGATGATGAAATAGAA GAAATGACAGAAACAAAAGAAACTGAGGAATCAGCCTTAGTATCACAGGCAGAAGCTCTGAAGGAAGAAAATGACAGCCTCCGTTGGCAGCTCGATGCCT ACCGGAATGAAGTAGAACTGCTCAAGCAAGAACAAGGCAAAGTCCACAGAGAAGATGACCCTAACAAAGAACAGCAGCTGAAACTCCTGCAACAAGCCCT GCAAGGAATGCAACAGCATCTACTCAAAGTCCAAGAGGAATACAAAAAGAAAGAAGCTGAACTTGAAAAACTCAAAGATGACAAGTTACAGGTGGAAAAA ATGTTGGAAAATCTTAAAGAAAAGGAAAGCTGTGCTTCTAGGCTGTGTGCCTCAAACCAGGATAGCGAATACCCTCTTGAGAAGACCATGAACAGCAGTC CTATCAAATCTGAACGTGAAGCACTGCTAGTGGGGATTATCTCCACATTCCTTCATGTTCACCCATTTGGAGCAAGCATTGAATACATCTGTTCCTACTT GCACCGTCTTGATAATAAGATCTGCACCAGCGATGTGGAGTGTCTCATGGGTAGACTCCAGCATACCTTCAAGCAGGAAATGACTGGAGTTGGAGCCAGC CTGGAAAAGAGATGGAAATTCTGTGGCTTCGAGGGCTTGAAGCTGACCTAAATCTCTTTGCCTAACAACTTGGGATCCTGAAGATAAATATGTGTTGGAC AAGCATAGAAAGTGATTTATATTTTTAATGGTTTTCAAGTGGAAGTTCCTTTGAATTTGTCAGTTCATTCCTGGAAAATCTTTTGAGTTAAAATAAGGAT CCTAGGACAGCACCTCGAACTACAGGCCCTAAAGAGAAATTGCCTCAAACCACAAGTGCTGTAACTTCCTCCCCTTTCTGTCAATTGGTTGTCTTTAAAT ATTGCAAAAGTCCTGATGCTAAACAGTATTTGGAGTGTTTTCAGTGTCTGTACTACTGTTGTAGACCTTGGTATTTTTTTAAACACTGTTAACTGAAATG TTTTGATGATTTGTATGTGATTTGTGTTTCTAAACTTCTCTTTACATTAATGTTGTTACTGGTGAAAGGCATGAGAGCAGCACTAAGTCCTCTGTGTAAC TGCCATTGTCTTTCCAATCCCCAGTAGACCAGTAAATAAATAACACATCAGTGTCTTCTAGAAGGTGCCTGACCAGGTTCACCTTTTAAACGACAAAGCA TGGTTTGTGGCTTTTTGCAAAATTACTATGAACCAAAAGTTGACAAATGTTCCAAAGTTATTTTCTCTAACATATCACATTAAAGATCTGTTTCAGAATT GTAAAAAGTACATCTAGATGTGTTTACAGAAAGCAAGTATCCAGTATGACTGGCATGTGTTCATGCTATTCAGAATCACTTGTAAATAGTCTGCTTTTAA AGGAGGGCATGTTCAGTTTTCTGTGAATTAAAATATGCTCATGTGTGGGCACACACGCACAAACACACACACGCACGCACACAGTGGCAGAAGGGATTTA TATTAATATTCTTTCCCCTCTGGCCTTCTTACAGTCTGTTGGTCCCTTTGCTTCTGTTGTCAGTGTGTTGAATTGCAAACCGAGTACTGCTGTAAATACT ATGTTTACTTCATGCTGAATGTTTGCAAAGACTTGATATAAGTATTAATAGTAATGAATCAATGAATAAATAATGAGCTAGGGTTTGTGAGGCTTTCTAC AAATAGGTCAGCTCCACCTGGAGTGCGAATTGCCAGAGACACCTTGGTAGTGCCCATCGGCAAATCGCAATGGCAGCATGTGAGTGGACCATTCAGAAAC TTCTGCTTGGTGGAAAGTAAACAGAGAGGATGGAGGTTTGGGGCGAATGTCCTGAGGCAGAGATGGTCTTTATTGTGTGTGGTGGTGGTTGTGGTATTTA TAATAATGCAAGCATACCCTCCCTTGAGTCTCAATTGAAGATAAAAGAATGTACTGAGCAAGCAAAGCCAATGGAGAGTATTTCACAAAAATACTTTGTA AATGAGATGCCAGTAGTGTTCAAAGTTGTATTTTTAAAAGATAAATATTCCTTTTTATACCTCAGTTTTGTGTCCTGTTTTTAATGACTTACGCTCTAAG TAATCCATTAGTAGTTATCTCAGTTCCTTCCTTTGGGTTACTAGAATGTTGGAAAAAGATGCCAAGTCTGTCTTGACAACTGGAAACAGGGTTCCACAGC AGCCCATTCGTGCTGAAAACTGGCTTCCCCCTGAAGCACCCTGCTGTGGCACCAGCAGGAAGCTCAGGTTAATTTTACACTAGCTTGCTCACTGATGCAT CTCTCATCAATGCTACGGAAGGCTTTGATTCATCAGTCTCGGGCTCTTGGAATACCTAATTTTAATAATATCTATGAAATCAAGGGAAACTTTCCATTTA CAGTTATTTCTTGTTTAAATAAACTAAATTAATTTTTAGGGGAGAGCAGTAGGAAAAAGAGCTAATGCATGCGGGGTTTAATACCTAGGTGATGGGTTGA GGTGCAGCAAAACCACCATGGCACACGTTCACCTATGTAACAAACCTGCACATCCTGCACATGTACCCCGGAACTTACTTAAAA

refseq_COVA1.F2 (forward primer)
refseq_COVA1.R2 (reverse primer)
Single exon skipping

Frame 0

VCESPALGQSAGSRPGSARRAFRKRVARRGAPR GHYEPRNCRAGPIFRGCIRTSASVAGELEGT*FE INSWRTQRSVMD*TSCPLTLLTTTSPCSLVVPV ALWSQKASLRMEGNQARKEREQILLQK**QS*V HS*GPHNAKRF*MAVGLRNRLCSR*Q*NSERGFH CNDTTYV*SNCMGHSNE*SWNGTAGNCRTTNFT *L*SCSWNDDWNSTNNSNDAWFGNSTSTNSSRY ASSKRDHTL*KLHALPSKSKSPTSCNPRKTTRMQ NSICGWSA*KWDRANHCGSFRAVWRDHCHSQEQ EELLPHSLC*GVHGGQSPVSVWLPHSPGL*Y*Q EGHRQTPR*FRTGSR*PV*VGV*TAYASQRGAPS *KNGRRKIASTISTPSGPLFRS*MQHCC*KIKR *FQILRSCTDLAYLDRARRGQPS*RQ*LLLHDP VGQQPCPPPGERESCP*ERYGRSKGEVQAGPFWN SHSI*ADSGCVPFRLQAEGMGPLHKSPAEEHQR VVQTS*GNSQHS***INGNQARRRNGNV***NR RNDRNKRN*GISLSITGRSSEGRK*QPPLAARCL PE*SRTAQARTRQSPQRR*P*QRTAAETPATSP ARNATASTQSPRGIQKERS*T*KTQR*QVTGGK NVGKS*RKGKLCF*AVCLKPG*RIPS*EDHEQQS YQI*T*STASGDYLHIPSCSPIWSKH*IHLFLL APS***DLHQRCGVSHG*TPAYLQAGNDWSWSQ PGKEMEILWLRGLEADLNLFA*QLGILKINMCWT SIESDLYF*WFSSGSSFEFVSSFLENLLS*NKD PRTAPRTTGPKEKLPQTTSAVTSSPFCQLVVFK YCKSPDAKQYLECFQCLYYCCRPWYFFKHC*LKC FDDLYVICVSKLLFTLMLLLVKGMRAALSPLCN CHCLSNPQ*TSK*ITHQCLLEGA*PGSPFKRQS MVCGFLQNYYEPKVDKCSKVIFSNISH*RSVSEL *KVHLDVFTESKYPV*LACVHAIQNHL*IVCF* RRACSVFCELKYAHVWAHTHKHTHARTQWQKGF ILIFFPLWPSYSLLVPLLLLSVC*IANRVLL*IL CLLHAECLQRLDISINSNESMNK**ARVCEAFY K*VSSTWSANCQRHLGSAHRQIAMAACEWTIQK LLLGGK*TERMEVWGECPEAEMVFIVCGGGCGIY NNASIPSLESQLKIKECTEQAKPMESISQKYFV NEMPVVFKVVFLKDKYSFLYLSFVSCF**LTL* VIH**LSQFLPLGY*NVGKRCQVCLDNWKQGSTA AHSC*KLASP*STLLWHQQEAQVNFTLACSLMH LSSMLRKALIHQSRALGIPNFNNIYEIKGNFPF TVISCLNKLN*FLGESSRKKS*CMRGLIPR*WVE VQQNHHGTRSPM*QTCTSCTCTPELT*

Frame 1

CVRVQPWARVPGLGLEARGGHFGREWRGVGRRE GTTSPGTAAPARFSAAV*GLALR*PGSWREPDL RSILGEHKEV*WIEPAAL*PC*QQRAPAVW*CQW PSGHRRPH*EWRGIKQEKKENKYSFRNDDRAKF TVEDHTMQRDFRWLWVYEIGYAADNSRTLNVDS TAMTLPMSDPTAWATAMNNLGMAPLGIAGQPILP DFDPALGMMTGIPPITPMMPGLGIVPPPIPPDM PVVKEIIHCKSCTLFPPNPNLPPPATRERPPGC KTVFVGGLPENGTEQIIVEVFEQCGEIIAIRKSK KNFCHIRFAEEYMVDKALYLSGYRIRLGSSTDK KDTGRLHVDFAQARDDLYEWECKQRMLAREERH RRRMEEERLRPPSPPPVVHYSDHECSIVAEKLKD DSKFSEAVQTLLTWIERGEVNRRSANNFYSMIQ SANSHVRRLVNEKAAHEKDMEEAKEKFKQALSG ILIQFEQIVAVYHSASKQKAWDHFTKAQRKNISV WCKQAEEIRNIHNDELMGIRREEEMEMSDDEIE EMTETKETEESALVSQAEALKEENDSLRWQLDA YRNEVELLKQEQGKVHREDDPNKEQQLKLLQQAL QGMQQHLLKVQEEYKKKEAELEKLKDDKLQVEK MLENLKEKESCASRLCASNQDSEYPLEKTMNSS PIKSEREALLVGIISTFLHVHPFGASIEYICSYL HRLDNKICTSDVECLMGRLQHTFKQEMTGVGAS LEKRWKFCGFEGLKLT*ISLPNNLGS*R*ICVG QA*KVIYIFNGFQVEVPLNLSVHSWKIF*VKIRI LGQHLELQALKRNCLKPQVL*LPPLSVNWLSLN IAKVLMLNSIWSVFSVCTTVVDLGIFLNTVN*N VLMICM*FVFLNFSLH*CCYW*KA*EQH*VLCVT AIVFPIPSRPVNK*HISVF*KVPDQVHLLNDKA WFVAFCKITMNQKLTNVPKLFSLTYHIKDLFQN CKKYI*MCLQKASIQYDWHVFMLFRITCK*SAFK GGHVQFSVN*NMLMCGHTRTNTHTHAHSGRRDL Y*YSFPSGLLTVCWSLCFCCQCVELQTEYCCKY YVYFMLNVCKDLI*VLIVMNQ*INNELGFVRLST NRSAPPGVRIARDTLVVPIGKSQWQHVSGPFRN FCLVESKQRGWRFGANVLRQRWSLLCVVVVVVF IIMQAYPPLSLN*R*KNVLSKQSQWRVFHKNTL* MRCQ*CSKLYF*KINIPFYTSVLCPVFNDLRSK *SISSYLSSFLWVTRMLEKDAKSVLTTGNRVPQ QPIRAENWLPPEAPCCGTSRKLRLILH*LAH*CI SHQCYGRL*FISLGLLEYLILIISMKSRETFHL QLFLV*IN*INF*GRAVGKRANACGV*YLGDGL RCSKTTMAHVHLCNKPAHPAHVPRNLLK

Frame 2

V*ESSLGPECRV*AWKREAGISEESGAAWGAAR ALRAQELPRRPDFPRLYKD*RFGSRGAGGNLI* DQFLENTKKCDGLNQLPFDPANNNEPLQFGSAS GPLVTEGLIENGGESSKKRKRTNTPSEMMTELSS QLRTTQCKEILDGCGSTK*AMQPITVEL*TWIP LQ*HYLCLIQLHGPQQ*IILEWHRWELPDNQFY LTLILLLE**LEFHQ*LQ*CLVWE*YLHQFLQIC Q**KRSYTVKAARSSLQIQISHLLQPEKDHQDA KQYLWVVCLKMGQSKSLWKFSSSVERSLPFARA RRTSATFALLRSTWWTKPCICLVTAFAWALVLTR RTQADSTLISHRLEMTCMSGSVNSVC*PERSAI VEEWKKKDCVHHLHPQWSTIQIMNAALLLKN*K MIPNSQKLYRPCLPG*SEERSTVVAPITSTP*SS RPTAMSAAW*TRKLPMRKIWKKQRRSSSRPFLE FSFNLSR*WLCTIPPPSRRHGTTSQKPSGRTSA CGANKLRKFATFIMMN*WESGEKKKWKCLMMK*K K*QKQKKLRNQP*YHRQKL*RKKMTASVGSSMP TGMK*NCSSKNKAKSTEKMTLTKNSS*NSCNKP CKECNSIYSKSKRNTKRKKLNLKNSKMTSYRWKK CWKILKKRKAVLLGCVPQTRIANTLLRRP*TAV LSNLNVKHC*WGLSPHSFMFTHLEQALNTSVPT CTVLIIRSAPAMWSVSWVDSSIPSSRK*LELEPA WKRDGNSVASRA*S*PKSLCLTTWDPEDKYVLD KHRK*FIFLMVFKWKFL*ICQFIPGKSFELK*G S*DSTSNYRP*REIASNHKCCNFLPFLSIGCL*I LQKS*C*TVFGVFSVSVLLL*TLVFF*TLLTEM F**FVCDLCF*TSLYINVVTGERHESSTKSSV* LPLSFQSPVDQ*INNTSVSSRRCLTRFTF*TTKH GLWLFAKLL*TKS*QMFQSYFL*HITLKICFRI VKSTSRCVYRKQVSSMTGMCSCYSESLVNSLLL KEGMFSFL*IKICSCVGTHAQTHTRTHTVAEGIY INILSPLAFLQSVGPFASVVSVLNCKPSTAVNT MFTSC*MFAKT*YKY****INE*IMS*GL*GFL QIGQLHLECELPETPW*CPSANRNGSM*VDHSET SAWWKVNREDGGLGRMS*GRDGLYCVWWWLWYL **CKHTLP*VSIEDKRMY*ASKANGEYFTKILC K*DASSVQSCIFKR*IFLFIPQFCVLFLMTYALS NPLVVISVPSFGLLECWKKMPSLS*QLETGFHS SPFVLKTGFPLKHPAVAPAGSSG*FYTSLLTDA SLINATEGFDSSVSGSWNT*F**YL*NQGKLSIY SYFLFK*TKLIFRGEQ*EKELMHAGFNT*VMG* GAAKPPWHTFTYVTNLHILHMYPGTYLK