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Sequence NM_022809
GGTCAACGCCTGCGGCTGTTGATATTCTTGCTCAGAGGCCGTAACTTTGGCCTTCTGCTCAGGGAAGACTCTGAGTCCGACGTTGGCCTACCCAGTCGGAAGGCAGAGCTGCAATCTAGTTAACTACCTCCTTTCCCCTAGATTTCCTTTCATTCTGCTCAAGTCTTCGCCTGTGTCCGATCCCTATCTACTTTCTCTCC
TCTTGTAGGCAAGCCTCAGACTCCAGGCTTGAGCTAGGTTTTGTTTTTCTCCTGGTGAGAATTCGAAGACCATGTCTACGGAACTCTTCTCATCCACAAG
AGAGGAAGGAAGCTCTGGCTCAGGACCCAGTTTTAGGTCTAATCAAAGGAAAATGTTAAACCTGCTCCTGGAGAGAGACACTTCCTTTACCGTCTGTCCA
GATGTCCCTAGAACTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTAAGCATTTTGTCTGGGTCACCTGGATTCTTCAGGACTTCAGGAAGTGCAT
TTAGCTGGGATGACAATGGAAACTTGGTGGACAGTGAAATGAAATATTTGGGCAGTCCCATTACTACTGTTCCAAAATTGGATAAAAATCCAAACCTAGG
AGAAGACCAGGCAGAAGAGATTTCAGATGAATTAATGGAGTTTTCCCTGAAAGATCAAGAAGCAAAGGTGAGCAGAAGTGGCCTATATCGCTCCCCGTCG
ATGCCAGAGAACTTGAACAGGCCAAGACTGAAGCAGGTGGAAAAATTCAAGGACAACACAATACCAGATAAAGTTAAAAAAAAGTATTTTTCTGGCCAAG
GAAAGCTCAGGAAGGGCTTATGTTTAAAGAAGACAGTCTCTCTGTGTGACATTACTATCACTCAGATGCTGGAGGAAGATTCTAACCAGGGGCACCTGAT
TGGTGATTTTTCCAAGGTATGTGCGCTGCCAACCGTGTCAGGGAAACACCAAGATCTGAAGTATGTCAACCCAGAAACAGTGGCTGCCTTACTGTCGGGG
AAGTTCCAGGGTCTGATTGAGAAGTTTTATGTCATTGATTGTCGCTATCCATATGAGTATCTGGGAGGACACATCCAGGGAGCCTTAAACTTATATAGTC
AGGAAGAACTGTTTAACTTCTTTCTGAAGAAGCCCATCGTCCCTTTGGACACCCAGAAGAGAATAATCATCGTGTTCCACTGTGAATTCTCCTCAGAGAG
GGGCCCCCGAATGTGCCGCTGTCTGCGTGAAGAGGACAGGTCTCTGAACCAGTATCCTGCATTGTACTACCCAGAGCTATATATCCTTAAAGGCGGCTAC
AGAGACTTCTTTCCAGAATATATGGAACTGTGTGAACCACAGAGCTACTGCCCTATGCATCATCAGGACCACAAGACTGAGTTGCTGAGGTGTCGAAGCC
AGAGCAAAGTGCAGGAAGGGGAGCGGCAGCTGCGGGAGCAGATTGCCCTTCTGGTGAAGGACATGAGCCCATGATAACATTCCAGCCACTGGCTGCTAAC
AAGTCACCAAAAGACACTGCAGAAACCCTGAGCAGAAAGAGGCCTTCTGGATGGCCAAACCCAAGATTATTAAAAGATGTCTCTGCAAACCAACAGGCTA
CCAACTTGTATCCAGGCCTGGGAATGGATTAGGTTTCAGCAGAGCTGAAAGCTGGTGGCAGAGTCCTGGAGCTGGCTCTATAAGGCAGCCTTGAGTTGCA
TAGAGATTTGTATTGGTTCAGGGAACTCTGGCATTCCTTTTCCCAACTCCTCATGTCTTCTCACAAGCCAGCCAACTCTTTCTCTCTGGGCTTCGGGCTA
TGCAAGAGCGTTGTCTACCTTCTTTCTTTGTATTTTCCTTCTTTGTTTCCCCCTCTTTCTTTTTTAAAAATGGAAAAATAAACACTACAGAATGAG
- refseq_CDC25C.F3 (forward primer)
- refseq_CDC25C.R3 (reverse primer)
- Multiple exon skipping
Frame 0
GQRLRLLIFLLRGRNFGLLLREDSESDVGLPSRKAELQSS*LPPFP*ISFHSAQVFACVRSLSTFSP
LVGKPQTPGLS*VLFFSW*EFEDHVYGTLLIHK
RGRKLWLRTQF*V*SKENVKPAPGERHFLYRLS
RCP*NSSGQISW*FCKPKHFVWVTWILQDFRKCI
*LG*QWKLGGQ*NEIFGQSHYYCSKIG*KSKPR
RRPGRRDFR*INGVFPERSRSKGEQKWPISLPV
DARELEQAKTEAGGKIQGQHNTR*S*KKVFFWPR
KAQEGLMFKEDSLSV*HYYHSDAGGRF*PGAPD
W*FFQGMCAANRVRETPRSEVCQPRNSGCLTVG
EVPGSD*EVLCH*LSLSI*VSGRTHPGSLKLI*S
GRTV*LLSEEAHRPFGHPEENNHRVPL*ILLRE
GPPNVPLSA*RGQVSEPVSCIVLPRAIYP*RRL
QRLLSRIYGTV*TTELLPYASSGPQD*VAEVSKP
EQSAGRGAAAAGADCPSGEGHEPMITFQPLAAN
KSPKDTAETLSRKRPSGWPNPRLLKDVSANQQA
TNLYPGLGMD*VSAELKAGGRVLELAL*GSLELH
RDLYWFRELWHSFSQLLMSSHKPANSFSLGFGL
CKSVVYLLSLYFPSLFPPLSFLKMEK*TLQNE
Frame 1
VNACGC*YSCSEAVTLAFCSGKTLSPTLAYPVGRQSCNLVNYLLSPRFPFILLKSSPVSDPYLLSL
LL*ASLRLQA*ARFCFSPGENSKTMSTELFSSTR
EEGSSGSGPSFRSNQRKMLNLLLERDTSFTVCP
DVPRTPVGKFLGDSANLSILSGSPGFFRTSGSA
FSWDDNGNLVDSEMKYLGSPITTVPKLDKNPNLG
EDQAEEISDELMEFSLKDQEAKVSRSGLYRSPS
MPENLNRPRLKQVEKFKDNTIPDKVKKKYFSGQ
GKLRKGLCLKKTVSLCDITITQMLEEDSNQGHLI
GDFSKVCALPTVSGKHQDLKYVNPETVAALLSG
KFQGLIEKFYVIDCRYPYEYLGGHIQGALNLYS
QEELFNFFLKKPIVPLDTQKRIIIVFHCEFSSER
GPRMCRCLREEDRSLNQYPALYYPELYILKGGY
RDFFPEYMELCEPQSYCPMHHQDHKTELLRCRS
QSKVQEGERQLREQIALLVKDMSP**HSSHWLLT
SHQKTLQKP*AERGLLDGQTQDY*KMSLQTNRL
PTCIQAWEWIRFQQS*KLVAESWSWLYKAALSC
IEICIGSGNSGIPFPNSSCLLTSQPTLSLWASGY
ARALSTFFLCIFLLCFPLFLF*KWKNKHYRM
Frame 2
STPAAVDILAQRP*LWPSAQGRL*VRRWPTQSEGRAAI*LTTSFPLDFLSFCSSLRLCPIPIYFLS
SCRQASDSRLELGFVFLLVRIRRPCLRNSSHPQ
ERKEALAQDPVLGLIKGKC*TCSWRETLPLPSVQ
MSLELQWANFLVILQT*AFCLGHLDSSGLQEVH
LAGMTMETWWTVK*NIWAVPLLLFQNWIKIQT*
EKTRQKRFQMN*WSFP*KIKKQR*AEVAYIAPRR
CQRT*TGQD*SRWKNSRTTQYQIKLKKSIFLAK
ESSGRAYV*RRQSLCVTLLSLRCWRKILTRGT*
LVIFPRYVRCQPCQGNTKI*SMSTQKQWLPYCRG
SSRV*LRSFMSLIVAIHMSIWEDTSREP*TYIV
RKNCLTSF*RSPSSLWTPRRE*SSCSTVNSPQR
GAPECAAVCVKRTGL*TSILHCTTQSYISLKAAT
ETSFQNIWNCVNHRATALCIIRTTRLSC*GVEA
RAKCRKGSGSCGSRLPFW*RT*AHDNIPATGC*
QVTKRHCRNPEQKEAFWMAKPKIIKRCLCKPTGY
QLVSRPGNGLGFSRAESWWQSPGAGSIRQP*VA
*RFVLVQGTLAFLFPTPHVFSQASQLFLSGLRA
MQERCLPSFFVFSFFVSPSFFFKNGKINTTE*
Sequence NM_001790
GGTCAACGCCTGCGGCTGTTGATATTCTTGCTCAGAGGCCGTAACTTTGGCCTTCTGCTCAGGGAAGACTCTGAGTCCGACGTTGGCCTACCCAGTCGGAAGGCAGAGCTGCAATCTAGTTAACTACCTCCTTTCCCCTAGATTTCCTTTCATTCTGCTCAAGTCTTCGCCTGTGTCCGATCCCTATCTACTTTCTCTCC
TCTTGTAGGCAAGCCTCAGACTCCAGGCTTGAGCTAGGTTTTGTTTTTCTCCTGGTGAGAATTCGAAGACCATGTCTACGGAACTCTTCTCATCCACAAG
AGAGGAAGGAAGCTCTGGCTCAGGACCCAGTTTTAGGTCTAATCAAAGGAAAATGTTAAACCTGCTCCTGGAGAGAGACACTTCCTTTACCGTCTGTCCA
GATGTCCCTAGAACTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTAAGCATTTTGTCTGGAGGAACCCCAAAACGTTGCCTCGATCTTTCGAATC
TTAGCAGTGGGGAGATAACTGCCACTCAGCTTACCACTTCTGCAGACCTTGATGAAACTGGTCACCTGGATTCTTCAGGACTTCAGGAAGTGCATTTAGC
TGGGATGAATCATGACCAGCACCTAATGAAATGTAGCCCAGCACAGCTTCTTTGTAGCACTCCGAATGGTTTGGACCGTGGCCATAGAAAGAGAGATGCA
ATGTGTAGTTCATCTGCAAATAAAGAAAATGACAATGGAAACTTGGTGGACAGTGAAATGAAATATTTGGGCAGTCCCATTACTACTGTTCCAAAATTGG
ATAAAAATCCAAACCTAGGAGAAGACCAGGCAGAAGAGATTTCAGATGAATTAATGGAGTTTTCCCTGAAAGATCAAGAAGCAAAGGTGAGCAGAAGTGG
CCTATATCGCTCCCCGTCGATGCCAGAGAACTTGAACAGGCCAAGACTGAAGCAGGTGGAAAAATTCAAGGACAACACAATACCAGATAAAGTTAAAAAA
AAGTATTTTTCTGGCCAAGGAAAGCTCAGGAAGGGCTTATGTTTAAAGAAGACAGTCTCTCTGTGTGACATTACTATCACTCAGATGCTGGAGGAAGATT
CTAACCAGGGGCACCTGATTGGTGATTTTTCCAAGGTATGTGCGCTGCCAACCGTGTCAGGGAAACACCAAGATCTGAAGTATGTCAACCCAGAAACAGT
GGCTGCCTTACTGTCGGGGAAGTTCCAGGGTCTGATTGAGAAGTTTTATGTCATTGATTGTCGCTATCCATATGAGTATCTGGGAGGACACATCCAGGGA
GCCTTAAACTTATATAGTCAGGAAGAACTGTTTAACTTCTTTCTGAAGAAGCCCATCGTCCCTTTGGACACCCAGAAGAGAATAATCATCGTGTTCCACT
GTGAATTCTCCTCAGAGAGGGGCCCCCGAATGTGCCGCTGTCTGCGTGAAGAGGACAGGTCTCTGAACCAGTATCCTGCATTGTACTACCCAGAGCTATA
TATCCTTAAAGGCGGCTACAGAGACTTCTTTCCAGAATATATGGAACTGTGTGAACCACAGAGCTACTGCCCTATGCATCATCAGGACCACAAGACTGAG
TTGCTGAGGTGTCGAAGCCAGAGCAAAGTGCAGGAAGGGGAGCGGCAGCTGCGGGAGCAGATTGCCCTTCTGGTGAAGGACATGAGCCCATGATAACATT
CCAGCCACTGGCTGCTAACAAGTCACCAAAAGACACTGCAGAAACCCTGAGCAGAAAGAGGCCTTCTGGATGGCCAAACCCAAGATTATTAAAAGATGTC
TCTGCAAACCAACAGGCTACCAACTTGTATCCAGGCCTGGGAATGGATTAGGTTTCAGCAGAGCTGAAAGCTGGTGGCAGAGTCCTGGAGCTGGCTCTAT
AAGGCAGCCTTGAGTTGCATAGAGATTTGTATTGGTTCAGGGAACTCTGGCATTCCTTTTCCCAACTCCTCATGTCTTCTCACAAGCCAGCCAACTCTTT
CTCTCTGGGCTTCGGGCTATGCAAGAGCGTTGTCTACCTTCTTTCTTTGTATTTTCCTTCTTTGTTTCCCCCTCTTTCTTTTTTAAAAATGGAAAAATAA
ACACTACAGAATGAG
- refseq_CDC25C.F3 (forward primer)
- refseq_CDC25C.R3 (reverse primer)
- Multiple exon skipping
Frame 0
GQRLRLLIFLLRGRNFGLLLREDSESDVGLPSRKAELQSS*LPPFP*ISFHSAQVFACVRSLSTFSP
LVGKPQTPGLS*VLFFSW*EFEDHVYGTLLIHK
RGRKLWLRTQF*V*SKENVKPAPGERHFLYRLS
RCP*NSSGQISW*FCKPKHFVWRNPKTLPRSFES
*QWGDNCHSAYHFCRP**NWSPGFFRTSGSAFS
WDES*PAPNEM*PSTASL*HSEWFGPWP*KERC
NV*FICK*RK*QWKLGGQ*NEIFGQSHYYCSKIG
*KSKPRRRPGRRDFR*INGVFPERSRSKGEQKW
PISLPVDARELEQAKTEAGGKIQGQHNTR*S*K
KVFFWPRKAQEGLMFKEDSLSV*HYYHSDAGGRF
*PGAPDW*FFQGMCAANRVRETPRSEVCQPRNS
GCLTVGEVPGSD*EVLCH*LSLSI*VSGRTHPG
SLKLI*SGRTV*LLSEEAHRPFGHPEENNHRVPL
*ILLREGPPNVPLSA*RGQVSEPVSCIVLPRAI
YP*RRLQRLLSRIYGTV*TTELLPYASSGPQD*
VAEVSKPEQSAGRGAAAAGADCPSGEGHEPMITF
QPLAANKSPKDTAETLSRKRPSGWPNPRLLKDV
SANQQATNLYPGLGMD*VSAELKAGGRVLELAL
*GSLELHRDLYWFRELWHSFSQLLMSSHKPANSF
SLGFGLCKSVVYLLSLYFPSLFPPLSFLKMEK*
TLQNE
Frame 1
VNACGC*YSCSEAVTLAFCSGKTLSPTLAYPVGRQSCNLVNYLLSPRFPFILLKSSPVSDPYLLSL
LL*ASLRLQA*ARFCFSPGENSKTMSTELFSSTR
EEGSSGSGPSFRSNQRKMLNLLLERDTSFTVCP
DVPRTPVGKFLGDSANLSILSGGTPKRCLDLSN
LSSGEITATQLTTSADLDETGHLDSSGLQEVHLA
GMNHDQHLMKCSPAQLLCSTPNGLDRGHRKRDA
MCSSSANKENDNGNLVDSEMKYLGSPITTVPKL
DKNPNLGEDQAEEISDELMEFSLKDQEAKVSRSG
LYRSPSMPENLNRPRLKQVEKFKDNTIPDKVKK
KYFSGQGKLRKGLCLKKTVSLCDITITQMLEED
SNQGHLIGDFSKVCALPTVSGKHQDLKYVNPETV
AALLSGKFQGLIEKFYVIDCRYPYEYLGGHIQG
ALNLYSQEELFNFFLKKPIVPLDTQKRIIIVFH
CEFSSERGPRMCRCLREEDRSLNQYPALYYPELY
ILKGGYRDFFPEYMELCEPQSYCPMHHQDHKTE
LLRCRSQSKVQEGERQLREQIALLVKDMSP**H
SSHWLLTSHQKTLQKP*AERGLLDGQTQDY*KMS
LQTNRLPTCIQAWEWIRFQQS*KLVAESWSWLY
KAALSCIEICIGSGNSGIPFPNSSCLLTSQPTL
SLWASGYARALSTFFLCIFLLCFPLFLF*KWKNK
HYRM
Frame 2
STPAAVDILAQRP*LWPSAQGRL*VRRWPTQSEGRAAI*LTTSFPLDFLSFCSSLRLCPIPIYFLS
SCRQASDSRLELGFVFLLVRIRRPCLRNSSHPQ
ERKEALAQDPVLGLIKGKC*TCSWRETLPLPSVQ
MSLELQWANFLVILQT*AFCLEEPQNVASIFRI
LAVGR*LPLSLPLLQTLMKLVTWILQDFRKCI*
LG*IMTST**NVAQHSFFVALRMVWTVAIEREMQ
CVVHLQIKKMTMETWWTVK*NIWAVPLLLFQNW
IKIQT*EKTRQKRFQMN*WSFP*KIKKQR*AEV
AYIAPRRCQRT*TGQD*SRWKNSRTTQYQIKLKK
SIFLAKESSGRAYV*RRQSLCVTLLSLRCWRKI
LTRGT*LVIFPRYVRCQPCQGNTKI*SMSTQKQ
WLPYCRGSSRV*LRSFMSLIVAIHMSIWEDTSRE
P*TYIVRKNCLTSF*RSPSSLWTPRRE*SSCST
VNSPQRGAPECAAVCVKRTGL*TSILHCTTQSY
ISLKAATETSFQNIWNCVNHRATALCIIRTTRLS
C*GVEARAKCRKGSGSCGSRLPFW*RT*AHDNI
PATGC*QVTKRHCRNPEQKEAFWMAKPKIIKRC
LCKPTGYQLVSRPGNGLGFSRAESWWQSPGAGSI
RQP*VA*RFVLVQGTLAFLFPTPHVFSQASQLF
LSGLRAMQERCLPSFFVFSFFVSPSFFFKNGKI
NTTE*
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