×
1 warning:
  • Access restricted to public data.
    Please sign in at Rnomics Platform to access your data.
Navigation
Sequences

Sequence NM_080539

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC
TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC
TTTCATCAACAGCGTTCTTTTCTTCAGAGGTGGCCGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCCCG
TGCATGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGGGAGCTTG
GCCGACCAGGAAGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAAGG
TGACCTGGGTATGATGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGGGT
GACCTGGGTCCCAAAGGAGAAAAGGGTTTCCCAGGATTTCCTGGAATGTTGGGGCAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGGAC
ACCGAGGACCCACAGGAAGACCAGGAAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTCTGGTCA
ACCTGGCCGTCCGGGGCCCCCAGGCCCCCCACCTGCAGGACAACTTATAATGGGACCCAAAGGGGAAAGAGGATTTCCCGGGCCTCCAGGAAGATGTCTT
TGTGGACCCACTATGAATGTGAATAACCCTTCCTACGGGGAATCTGTGTATGGGCCCAGTTCCCCGCGAGTTCCTGTGATTTTTGTGGTCAACAACCAGG
AGGAGCTTGAGAGGCTGAACACCCAAAACGCCATTGCCTTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGACAGCCTTGGCTGGCTCCCCATCCAGCT
GACCCCTTTCTACCCTGTGGATTACACTGCAGACCAGCACGGCACCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGTGACGACGGTAACAGCGAT
GTGGGTGACGACTGCATCCGCTGTCACCGTGCCTACTGTGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCTCTGACTTTGGCTACCTGA
CATGCGAGACCTATCTCCCTGGGTCATATGGAGACCTGCAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTACTTCACCTGAGGGCCGTG
AGGAGAAGGTGGGCTGCGCCCCACAGAACTGGCAGCAGCTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCCACCAACCTTTGTGTGACA
AAAACAAGGACAAATTCCTGCTACTAAGACATGCTGTTAACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCTTAATCCAAGGTACTGGAA
AACCTTCCACATGCCCAACCTGAGACCTGTTCCCCATCGCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTCTGGAATGTTGCTGACCCA
GGCCGTGGACTTGGCTGAGCACTGTTAGACACACACAGACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCTGGAGTACAGGCCTCTCCG
CCCCTGACCACCGAAACGTGCAGGCATTCTCACTCACACTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAAAGCAGGGAAGTGGGCACG
TTCTCTCGGGGTGGCTCACAGCTTTGAACCTGCCAAAGGACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGTGGCTGCCCAGGAAAAGAC
TTAACTGTGAAAAAGTACTGAGAACCCACCTGACCCAGGCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGTGTTTCCCAAAGGGGCGGC
TCTTGTGGTTTCAAAATCTCTGGCACCATCTTGACCTCTTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTCATGTCCATTTCCTGTCCA
GGAGACTCATGAGGACTGTGTGACCTGCACAAGCCCACACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATACTCCCCTGAGTCCCCGATC
TAGGGCCAGCTGCAGAGGGCTCTCTCTAGGCAGAGCGTTCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGACAGCGTCCCGTGGCATTTC
TTTCATGTCTGCATATTGCATAGCCTTGTCCTCCTGTGTGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATGTCTGTGAGCTGCCTTTCA
TCACCATTTTTCCTGAGTAGGGCTTAGTTTTATTCTGGAAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAAGTAGAAATTGCCCAATTT
TTACCAGCTGGAGGGACACCCTTGGGTTTTTGTACGAAGCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGCTTGGGGCCTGGTGGACCA
TCACCTGGTGTCTGTAGGTGGACCCGGTCTCCCACAGGTGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGGCAGCCCTGTTCCCTTCGC
CTCTACTGCGAGGCCTGGGGAGATGTTGTTTTCATGCTGCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAATAAAGGCAGTTCATTTCC
CCA
  • refseq_COLQ.F2 (forward primer)
  • refseq_COLQ.R2 (reverse primer)
  • Alternative donor and acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF*
LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT
FINSVLFFRGGRSPLLSPDMKNLMLELETSQSP
CMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGEL
GRPGRKGRPGPPGVPGMPGPIGWPGPEGPRGEKG
DLGMMGLPGSRGPMGSKGYPGSRGEKGSRGEKG
DLGPKGEKGFPGFPGMLGQKGEMGPKGEPGIAG
HRGPTGRPGKRGKQGQKGDSGVMGPPGKPGPSGQ
PGRPGPPGPPPAGQLIMGPKGERGFPGPPGRCL
CGPTMNVNNPSYGESVYGPSSPRVPVIFVVNNQ
EELERLNTQNAIAFRRDQRSLYFKDSLGWLPIQL
TPFYPVDYTADQHGTCGDGLLQPGEECDDGNSD
VGDDCIRCHRAYCGDGHRHEGVEDCDGSDFGYL
TCETYLPGSYGDLQCTQYCYIDSTPCRYFT*GP*
GEGGLRPTELAAASPLSSNWPCPGPVSTNLCVT
KTRTNSCY*DMLLTQSDWKNLGPLHPVLIQGTG
KPSTCPT*DLFPIATIWPNNRLQCSSSSGMLLTQ
AVDLAEHC*THTDLQAVLECFDLFGESGVQASP
PLTTETCRHSHSHWAARCRVSLGL*TTKQGSGH
VLSGWLTALNLPKDPSTGHSPAQPDVDVAAQEKT
*L*KSTENPPDPGLPQAEAREEAPLLSVSQRGG
SCGFKISGTILTSWLSLHFAPCLKNVPHVHFLS
RRLMRTV*PAQAHTWAGCWCLLGP*ADTPLSPRS
RASCRGLSLGRAFLARALPLCLLLTPDSVPWHF
FHVCILHSLVLLCA*APPFSIRLLVVHVCELPF
ITIFPE*GLVLFWKDISKVRSTPTADLK*KLPNF
YQLEGHPWVFVRSYLMSLCLGDSAGWSLGPGGP
SPGVCRWTRSPTGDINLRVASLETGTWAALFPS
PLLRGLGRCCFHAASTITLGFLDGK*KNKGSSFP

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN
LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR
LSSTAFFSSEVAEVRFSPQT*RISCWNWRPRSPR
ACKAR*APLGLPAPRVHRGFLARQDQREKRGSL
ADQEGRVDLAPQVFLACLGPSVGQALKDPGVKK
VTWV*WACQGQEDQWAPRATLDPEGKRDPEVKRV
TWVPKEKRVSQDFLECWGRKVKWVQKVNLG*QD
TEDPQEDQENEASRDRKGIVELWAHQASLGLLV
NLAVRGPQAPHLQDNL*WDPKGKEDFPGLQEDVF
VDPL*M*ITLPTGNLCMGPVPREFL*FLWSTTR
RSLRG*TPKTPLPSAETRDLCTSRTALAGSPSS
*PLSTLWITLQTSTAPVGMGSCSLGRSVTTVTAM
WVTTASAVTVPTVEMVTGMRVWRTVTALTLAT*
HARPISLGHMETCNAPSTATSTPRPAATSPEGR
EEKVGCAPQNWQQLLHCHQTGHVLAPSPPTFV*Q
KQGQIPATKTCC*LSLTGRI*DHCILS*SKVLE
NLPHAQPETCSPSLPFGQTTGYNALPLLECC*P
RPWTWLSTVRHTQTCRLF*NVLISLGNLEYRPLR
P*PPKRAGILTHTGQPAVGSL*AYEPQSREVGT
FSRGGSQL*TCQRTPRLATAQPSLTWMWLPRKR
LNCEKVLRTHLTQACPKQRLEKRLLFSVFPKGAA
LVVSKSLAPS*PLGSLCTLPPVSKMSLMSISCP
GDS*GLCDLHKPTPGQAVGVSLVLRQILP*VPD
LGPAAEGSL*AERSWPELYLFASC*PLTASRGIS
FMSAYCIALSSCVPELLPFQ*DY*SCMSVSCLS
SPFFLSRA*FYSGKTSPR*GPPPQQTSSRNCPI
FTSWRDTLGFLYEAI**ACVLGTQQAGAWGLVDH
HLVSVGGPGLPQVTST*GWRL*RQAHGQPCSLR
LYCEAWGDVVFMLLPPSHWGFWMGNKKIKAVHF
P

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT
*PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD
FHQQRSFLQRWPKSASLPRHEESHAGTGDLAVP
VHARLARLPWASRPPGSTGASWQDRTKGRKGGAW
PTRKEG*TWPPRCSWHAWAHRLARP*RTQG*KR
*PGYDGLARVKRTNGLQGLPWIQRGKGIQR*KG
*PGSQRRKGFPRISWNVGAER*NGSKR*TWDSRT
PRTHRKTRKTRQAGTERG*WSYGPTRQAWAFWS
TWPSGAPRPPTCRTTYNGTQRGKRISRASRKMS
LWTHYECE*PFLRGICVWAQFPASSCDFCGQQPG
GA*EAEHPKRHCLPQRPEISVLQGQPWLAPHPA
DPFLPCGLHCRPARHLWGWAPAAWGGV*RR*QR
CG*RLHPLSPCLLWRWSPA*GCGGL*RL*LWLPD
MRDLSPWVIWRPAMHPVLLHRLHALPLLHLRAV
RRRWAAPHRTGSSFSTVIKLAMSWPRLHQPLCD
KNKDKFLLLRHAVNSV*LEEFRTTASCLNPRYWK
TFHMPNLRPVPHRYHLAKQPATMLFLFWNVADP
GRGLG*ALLDTHRPAGCFRMF*SLWGIWSTGLS
APDHRNVQAFSLTLGSPLSGLSRPMNHKAGKWAR
SLGVAHSFEPAKGPLDWPQPSPA*RGCGCPGKD
LTVKKY*EPT*PRLAPSRG*RRGSSSQCFPKGR
LLWFQNLWHHLDLLALSALCPLSQKCPSCPFPVQ
ETHEDCVTCTSPHLGRLLVSPWSLGRYSPESPI
*GQLQRALSRQSVPGQSSTSLPPADP*QRPVAF
LSCLHIA*PCPPVCLSSSLFNKIISRACL*AAFH
HHFS*VGLSFILERHLQGEVHPHSRPQVEIAQF
LPAGGTPLGFCTKLFNEPVSWGLSRLELGAWWT
ITWCL*VDPVSHR*HQPEGGVFRDRHMGSPVPFA
STARPGEMLFSCCFHHHTGVSGWEIKK*RQFIS
P

Sequence NM_080542

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC
TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC
TTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGGCCACAAAGCATGCTGCCTGCTGACGCCTCCTCCA
CCACCACTGTTCCCACCACCA
TTCTTCAGAGGTGGCCGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCC
CGTGCATGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGGGAGCT
TGGCCGACCAGGAAGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAA
GGTGACCTGGGTATGATGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGG
GTGACCTGGGTCCCAAAAGTTCAAGAACTCCTTGCACATTACCAAGACGGCCCCCAGTGCCATGTGGACAAGGCAGCCGGTCACCTGTCACCGTTGTGGC
TGGAAAGGAGTCCCAAGCCTGCCTGCTCCCCAGATTTGAGGAAGATTACATTTCATCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAA
GCCTGGGCCTTCTGGTCAACCTGGCCGTCCGGGGCCCCCAGGCCCCCCACCTGCAGGACAACTTATAATGGGACCCAAAGGGGAAAGAGGATTTCCCGGG
CCTCCAGGAAGATGTCTTTGTGGACCCACTATGAATGTGAATAACCCTTCCTACGGGGAATCTGTGTATGGGCCCAGTTCCCCGCGAGTTCCTGTGATTT
TTGTGGTCAACAACCAGGAGGAGCTTGAGAGGCTGAACACCCAAAACGCCATTGCCTTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGACAGCCTTGG
CTGGCTCCCCATCCAGCTGACCCCTTTCTACCCTGTGGATTACACTGCAGACCAGCACGGCACCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGT
GACGACGGTAACAGCGATGTGGGTGACGACTGCATCCGCTGTCACCGTGCCTACTGTGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCT
CTGACTTTGGCTACCTGACATGCGAGACCTATCTCCCTGGGTCATATGGAGACCTGCAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTA
CTTCACCTGAGGGCCGTGAGGAGAAGGTGGGCTGCGCCCCACAGAACTGGCAGCAGCTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCC
ACCAACCTTTGTGTGACAAAAACAAGGACAAATTCCTGCTACTAAGACATGCTGTTAACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCT
TAATCCAAGGTACTGGAAAACCTTCCACATGCCCAACCTGAGACCTGTTCCCCATCGCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTC
TGGAATGTTGCTGACCCAGGCCGTGGACTTGGCTGAGCACTGTTAGACACACACAGACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCT
GGAGTACAGGCCTCTCCGCCCCTGACCACCGAAACGTGCAGGCATTCTCACTCACACTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAA
AGCAGGGAAGTGGGCACGTTCTCTCGGGGTGGCTCACAGCTTTGAACCTGCCAAAGGACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGT
GGCTGCCCAGGAAAAGACTTAACTGTGAAAAAGTACTGAGAACCCACCTGACCCAGGCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGT
GTTTCCCAAAGGGGCGGCTCTTGTGGTTTCAAAATCTCTGGCACCATCTTGACCTCTTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTC
ATGTCCATTTCCTGTCCAGGAGACTCATGAGGACTGTGTGACCTGCACAAGCCCACACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATAC
TCCCCTGAGTCCCCGATCTAGGGCCAGCTGCAGAGGGCTCTCTCTAGGCAGAGCGTTCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGAC
AGCGTCCCGTGGCATTTCTTTCATGTCTGCATATTGCATAGCCTTGTCCTCCTGTGTGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATG
TCTGTGAGCTGCCTTTCATCACCATTTTTCCTGAGTAGGGCTTAGTTTTATTCTGGAAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAA
GTAGAAATTGCCCAATTTTTACCAGCTGGAGGGACACCCTTGGGTTTTTGTACGAAGCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGC
TTGGGGCCTGGTGGACCATCACCTGGTGTCTGTAGGTGGACCCGGTCTCCCACAGGTGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGG
CAGCCCTGTTCCCTTCGCCTCTACTGCGAGGCCTGGGGAGATGTTGTTTTCATGCTGCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAA
TAAAGGCAGTTCATTTCCCCA
  • refseq_COLQ.F2 (forward primer)
  • refseq_COLQ.R2 (reverse primer)
  • Alternative donor and acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF*
LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT
FINSVLPISAALPSLDQKKRGGHKACCLLTPPP
PPLFPPP
FFRGGRSPLLSPDMKNLMLELETSQS
PCMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGEL
GRPGRKGRPGPPGVPGMPGPIGWPGPEGPRGEK
GDLGMMGLPGSRGPMGSKGYPGSRGEKGSRGEK
GDLGPKSSRTPCTLPRRPPVPCGQGSRSPVTVVA
GKESQACLLPRFEEDYISSGTERG*WSYGPTRQ
AWAFWSTWPSGAPRPPTCRTTYNGTQRGKRISR
ASRKMSLWTHYECE*PFLRGICVWAQFPASSCDF
CGQQPGGA*EAEHPKRHCLPQRPEISVLQGQPW
LAPHPADPFLPCGLHCRPARHLWGWAPAAWGGV
*RR*QRCG*RLHPLSPCLLWRWSPA*GCGGL*RL
*LWLPDMRDLSPWVIWRPAMHPVLLHRLHALPL
LHLRAVRRRWAAPHRTGSSFSTVIKLAMSWPRL
HQPLCDKNKDKFLLLRHAVNSV*LEEFRTTASCL
NPRYWKTFHMPNLRPVPHRYHLAKQPATMLFLF
WNVADPGRGLG*ALLDTHRPAGCFRMF*SLWGI
WSTGLSAPDHRNVQAFSLTLGSPLSGLSRPMNHK
AGKWARSLGVAHSFEPAKGPLDWPQPSPA*RGC
GCPGKDLTVKKY*EPT*PRLAPSRG*RRGSSSQ
CFPKGRLLWFQNLWHHLDLLALSALCPLSQKCPS
CPFPVQETHEDCVTCTSPHLGRLLVSPWSLGRY
SPESPI*GQLQRALSRQSVPGQSSTSLPPADP*
QRPVAFLSCLHIA*PCPPVCLSSSLFNKIISRAC
L*AAFHHHFS*VGLSFILERHLQGEVHPHSRPQ
VEIAQFLPAGGTPLGFCTKLFNEPVSWGLSRLE
LGAWWTITWCL*VDPVSHR*HQPEGGVFRDRHMG
SPVPFASTARPGEMLFSCCFHHHTGVSGWEIKK
*RQFISP

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN
LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR
LSSTAFFQSQQPFPAWIRRSVVATKHAAC*RLLH
HHCSHHH
SSEVAEVRFSPQT*RISCWNWRPRSP
RACKAR*APLGLPAPRVHRGFLARQDQREKRGS
LADQEGRVDLAPQVFLACLGPSVGQALKDPGVKK
VTWV*WACQGQEDQWAPRATLDPEGKRDPEVKR
VTWVPKVQELLAHYQDGPQCHVDKAAGHLSPLW
LERSPKPACSPDLRKITFHQGQKGDSGVMGPPGK
PGPSGQPGRPGPPGPPPAGQLIMGPKGERGFPG
PPGRCLCGPTMNVNNPSYGESVYGPSSPRVPVI
FVVNNQEELERLNTQNAIAFRRDQRSLYFKDSLG
WLPIQLTPFYPVDYTADQHGTCGDGLLQPGEEC
DDGNSDVGDDCIRCHRAYCGDGHRHEGVEDCDG
SDFGYLTCETYLPGSYGDLQCTQYCYIDSTPCRY
FT*GP*GEGGLRPTELAAASPLSSNWPCPGPVS
TNLCVTKTRTNSCY*DMLLTQSDWKNLGPLHPV
LIQGTGKPSTCPT*DLFPIATIWPNNRLQCSSSS
GMLLTQAVDLAEHC*THTDLQAVLECFDLFGES
GVQASPPLTTETCRHSHSHWAARCRVSLGL*TT
KQGSGHVLSGWLTALNLPKDPSTGHSPAQPDVDV
AAQEKT*L*KSTENPPDPGLPQAEAREEAPLLS
VSQRGGSCGFKISGTILTSWLSLHFAPCLKNVP
HVHFLSRRLMRTV*PAQAHTWAGCWCLLGP*ADT
PLSPRSRASCRGLSLGRAFLARALPLCLLLTPD
SVPWHFFHVCILHSLVLLCA*APPFSIRLLVVH
VCELPFITIFPE*GLVLFWKDISKVRSTPTADLK
*KLPNFYQLEGHPWVFVRSYLMSLCLGDSAGWS
LGPGGPSPGVCRWTRSPTGDINLRVASLETGTW
AALFPSPLLRGLGRCCFHAASTITLGFLDGK*KN
KGSSFP

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT
*PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD
FHQQRSSNLSSPSQPGSEEAWWPQSMLPADASS
TTTVPTT
ILQRWPKSASLPRHEESHAGTGDLAVP
VHARLARLPWASRPPGSTGASWQDRTKGRKGGA
WPTRKEG*TWPPRCSWHAWAHRLARP*RTQG*K
R*PGYDGLARVKRTNGLQGLPWIQRGKGIQR*KG
*PGSQKFKNSLHITKTAPSAMWTRQPVTCHRCG
WKGVPSLPAPQI*GRLHFIRDRKGIVELWAHQA
SLGLLVNLAVRGPQAPHLQDNL*WDPKGKEDFPG
LQEDVFVDPL*M*ITLPTGNLCMGPVPREFL*F
LWSTTRRSLRG*TPKTPLPSAETRDLCTSRTAL
AGSPSS*PLSTLWITLQTSTAPVGMGSCSLGRSV
TTVTAMWVTTASAVTVPTVEMVTGMRVWRTVTA
LTLAT*HARPISLGHMETCNAPSTATSTPRPAA
TSPEGREEKVGCAPQNWQQLLHCHQTGHVLAPSP
PTFV*QKQGQIPATKTCC*LSLTGRI*DHCILS
*SKVLENLPHAQPETCSPSLPFGQTTGYNALPL
LECC*PRPWTWLSTVRHTQTCRLF*NVLISLGNL
EYRPLRP*PPKRAGILTHTGQPAVGSL*AYEPQ
SREVGTFSRGGSQL*TCQRTPRLATAQPSLTWM
WLPRKRLNCEKVLRTHLTQACPKQRLEKRLLFSV
FPKGAALVVSKSLAPS*PLGSLCTLPPVSKMSL
MSISCPGDS*GLCDLHKPTPGQAVGVSLVLRQI
LP*VPDLGPAAEGSL*AERSWPELYLFASC*PLT
ASRGISFMSAYCIALSSCVPELLPFQ*DY*SCM
SVSCLSSPFFLSRA*FYSGKTSPR*GPPPQQTS
SRNCPIFTSWRDTLGFLYEAI**ACVLGTQQAGA
WGLVDHHLVSVGGPGLPQVTST*GWRL*RQAHG
QPCSLRLYCEAWGDVVFMLLPPSHWGFWMGNKK
IKAVHFP

Sequence NM_005677

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC
TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC
TTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGGCCACAAAGCATGCTGCCTGCTGACGCCTCCTCCA
CCACCACTGTTCCCACCACCA
TTCTTCAGAGGTGGCCGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCC
CGTGCATGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGGGAGCT
TGGCCGACCAGGAAGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAA
GGTGACCTGGGTATGATGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGG
GTGACCTGGGTCCCAAAGGAGAAAAGGGTTTCCCAGGATTTCCTGGAATGTTGGGGCAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGG
ACACCGAGGACCCACAGGAAGACCAGGAAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTCTGGT
CAACCTGGCCGTCCGGGGCCCCCAGGCCCCCCACCTGCAGGACAACTTATAATGGGACCCAAAGGGGAAAGAGGATTTCCCGGGCCTCCAGGAAGATGTC
TTTGTGGACCCACTATGAATGTGAATAACCCTTCCTACGGGGAATCTGTGTATGGGCCCAGTTCCCCGCGAGTTCCTGTGATTTTTGTGGTCAACAACCA
GGAGGAGCTTGAGAGGCTGAACACCCAAAACGCCATTGCCTTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGACAGCCTTGGCTGGCTCCCCATCCAG
CTGACCCCTTTCTACCCTGTGGATTACACTGCAGACCAGCACGGCACCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGTGACGACGGTAACAGCG
ATGTGGGTGACGACTGCATCCGCTGTCACCGTGCCTACTGTGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCTCTGACTTTGGCTACCT
GACATGCGAGACCTATCTCCCTGGGTCATATGGAGACCTGCAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTACTTCACCTGAGGGCCG
TGAGGAGAAGGTGGGCTGCGCCCCACAGAACTGGCAGCAGCTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCCACCAACCTTTGTGTGA
CAAAAACAAGGACAAATTCCTGCTACTAAGACATGCTGTTAACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCTTAATCCAAGGTACTGG
AAAACCTTCCACATGCCCAACCTGAGACCTGTTCCCCATCGCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTCTGGAATGTTGCTGACC
CAGGCCGTGGACTTGGCTGAGCACTGTTAGACACACACAGACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCTGGAGTACAGGCCTCTC
CGCCCCTGACCACCGAAACGTGCAGGCATTCTCACTCACACTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAAAGCAGGGAAGTGGGCA
CGTTCTCTCGGGGTGGCTCACAGCTTTGAACCTGCCAAAGGACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGTGGCTGCCCAGGAAAAG
ACTTAACTGTGAAAAAGTACTGAGAACCCACCTGACCCAGGCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGTGTTTCCCAAAGGGGCG
GCTCTTGTGGTTTCAAAATCTCTGGCACCATCTTGACCTCTTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTCATGTCCATTTCCTGTC
CAGGAGACTCATGAGGACTGTGTGACCTGCACAAGCCCACACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATACTCCCCTGAGTCCCCGA
TCTAGGGCCAGCTGCAGAGGGCTCTCTCTAGGCAGAGCGTTCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGACAGCGTCCCGTGGCATT
TCTTTCATGTCTGCATATTGCATAGCCTTGTCCTCCTGTGTGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATGTCTGTGAGCTGCCTTT
CATCACCATTTTTCCTGAGTAGGGCTTAGTTTTATTCTGGAAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAAGTAGAAATTGCCCAAT
TTTTACCAGCTGGAGGGACACCCTTGGGTTTTTGTACGAAGCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGCTTGGGGCCTGGTGGAC
CATCACCTGGTGTCTGTAGGTGGACCCGGTCTCCCACAGGTGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGGCAGCCCTGTTCCCTTC
GCCTCTACTGCGAGGCCTGGGGAGATGTTGTTTTCATGCTGCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAATAAAGGCAGTTCATTT
CCCCA
  • refseq_COLQ.F2 (forward primer)
  • refseq_COLQ.R2 (reverse primer)
  • Alternative donor and acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF*
LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT
FINSVLPISAALPSLDQKKRGGHKACCLLTPPP
PPLFPPP
FFRGGRSPLLSPDMKNLMLELETSQS
PCMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGEL
GRPGRKGRPGPPGVPGMPGPIGWPGPEGPRGEK
GDLGMMGLPGSRGPMGSKGYPGSRGEKGSRGEK
GDLGPKGEKGFPGFPGMLGQKGEMGPKGEPGIAG
HRGPTGRPGKRGKQGQKGDSGVMGPPGKPGPSG
QPGRPGPPGPPPAGQLIMGPKGERGFPGPPGRC
LCGPTMNVNNPSYGESVYGPSSPRVPVIFVVNNQ
EELERLNTQNAIAFRRDQRSLYFKDSLGWLPIQ
LTPFYPVDYTADQHGTCGDGLLQPGEECDDGNS
DVGDDCIRCHRAYCGDGHRHEGVEDCDGSDFGYL
TCETYLPGSYGDLQCTQYCYIDSTPCRYFT*GP
*GEGGLRPTELAAASPLSSNWPCPGPVSTNLCV
TKTRTNSCY*DMLLTQSDWKNLGPLHPVLIQGTG
KPSTCPT*DLFPIATIWPNNRLQCSSSSGMLLT
QAVDLAEHC*THTDLQAVLECFDLFGESGVQAS
PPLTTETCRHSHSHWAARCRVSLGL*TTKQGSGH
VLSGWLTALNLPKDPSTGHSPAQPDVDVAAQEK
T*L*KSTENPPDPGLPQAEAREEAPLLSVSQRG
GSCGFKISGTILTSWLSLHFAPCLKNVPHVHFLS
RRLMRTV*PAQAHTWAGCWCLLGP*ADTPLSPR
SRASCRGLSLGRAFLARALPLCLLLTPDSVPWH
FFHVCILHSLVLLCA*APPFSIRLLVVHVCELPF
ITIFPE*GLVLFWKDISKVRSTPTADLK*KLPN
FYQLEGHPWVFVRSYLMSLCLGDSAGWSLGPGG
PSPGVCRWTRSPTGDINLRVASLETGTWAALFPS
PLLRGLGRCCFHAASTITLGFLDGK*KNKGSSF
P

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN
LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR
LSSTAFFQSQQPFPAWIRRSVVATKHAAC*RLLH
HHCSHHH
SSEVAEVRFSPQT*RISCWNWRPRSP
RACKAR*APLGLPAPRVHRGFLARQDQREKRGS
LADQEGRVDLAPQVFLACLGPSVGQALKDPGVKK
VTWV*WACQGQEDQWAPRATLDPEGKRDPEVKR
VTWVPKEKRVSQDFLECWGRKVKWVQKVNLG*Q
DTEDPQEDQENEASRDRKGIVELWAHQASLGLLV
NLAVRGPQAPHLQDNL*WDPKGKEDFPGLQEDV
FVDPL*M*ITLPTGNLCMGPVPREFL*FLWSTT
RRSLRG*TPKTPLPSAETRDLCTSRTALAGSPSS
*PLSTLWITLQTSTAPVGMGSCSLGRSVTTVTA
MWVTTASAVTVPTVEMVTGMRVWRTVTALTLAT
*HARPISLGHMETCNAPSTATSTPRPAATSPEGR
EEKVGCAPQNWQQLLHCHQTGHVLAPSPPTFV*
QKQGQIPATKTCC*LSLTGRI*DHCILS*SKVL
ENLPHAQPETCSPSLPFGQTTGYNALPLLECC*P
RPWTWLSTVRHTQTCRLF*NVLISLGNLEYRPL
RP*PPKRAGILTHTGQPAVGSL*AYEPQSREVG
TFSRGGSQL*TCQRTPRLATAQPSLTWMWLPRKR
LNCEKVLRTHLTQACPKQRLEKRLLFSVFPKGA
ALVVSKSLAPS*PLGSLCTLPPVSKMSLMSISC
PGDS*GLCDLHKPTPGQAVGVSLVLRQILP*VPD
LGPAAEGSL*AERSWPELYLFASC*PLTASRGI
SFMSAYCIALSSCVPELLPFQ*DY*SCMSVSCL
SSPFFLSRA*FYSGKTSPR*GPPPQQTSSRNCPI
FTSWRDTLGFLYEAI**ACVLGTQQAGAWGLVD
HHLVSVGGPGLPQVTST*GWRL*RQAHGQPCSL
RLYCEAWGDVVFMLLPPSHWGFWMGNKKIKAVHF
P

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT
*PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD
FHQQRSSNLSSPSQPGSEEAWWPQSMLPADASS
TTTVPTT
ILQRWPKSASLPRHEESHAGTGDLAVP
VHARLARLPWASRPPGSTGASWQDRTKGRKGGA
WPTRKEG*TWPPRCSWHAWAHRLARP*RTQG*K
R*PGYDGLARVKRTNGLQGLPWIQRGKGIQR*KG
*PGSQRRKGFPRISWNVGAER*NGSKR*TWDSR
TPRTHRKTRKTRQAGTERG*WSYGPTRQAWAFW
STWPSGAPRPPTCRTTYNGTQRGKRISRASRKMS
LWTHYECE*PFLRGICVWAQFPASSCDFCGQQP
GGA*EAEHPKRHCLPQRPEISVLQGQPWLAPHP
ADPFLPCGLHCRPARHLWGWAPAAWGGV*RR*QR
CG*RLHPLSPCLLWRWSPA*GCGGL*RL*LWLP
DMRDLSPWVIWRPAMHPVLLHRLHALPLLHLRA
VRRRWAAPHRTGSSFSTVIKLAMSWPRLHQPLCD
KNKDKFLLLRHAVNSV*LEEFRTTASCLNPRYW
KTFHMPNLRPVPHRYHLAKQPATMLFLFWNVAD
PGRGLG*ALLDTHRPAGCFRMF*SLWGIWSTGLS
APDHRNVQAFSLTLGSPLSGLSRPMNHKAGKWA
RSLGVAHSFEPAKGPLDWPQPSPA*RGCGCPGK
DLTVKKY*EPT*PRLAPSRG*RRGSSSQCFPKGR
LLWFQNLWHHLDLLALSALCPLSQKCPSCPFPV
QETHEDCVTCTSPHLGRLLVSPWSLGRYSPESP
I*GQLQRALSRQSVPGQSSTSLPPADP*QRPVAF
LSCLHIA*PCPPVCLSSSLFNKIISRACL*AAF
HHHFS*VGLSFILERHLQGEVHPHSRPQVEIAQ
FLPAGGTPLGFCTKLFNEPVSWGLSRLELGAWWT
ITWCL*VDPVSHR*HQPEGGVFRDRHMGSPVPF
ASTARPGEMLFSCCFHHHTGVSGWEIKK*RQFI
SP

Sequence NM_080543

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC
TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC
TTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGGCCACAAAGCATGCTGCCTGCTGACGCCTCCTCCA
CCACCACTGTTCCCACCACCA
TTCTTCAGAGGTGGCCGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCC
CGTGCATGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGGGAGCT
TGGCCGACCAGGAAGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAA
GGTGACCTGGGTATGATGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGG
GTGACCTGGGTCCCAAAGGAGAAAAGGGTTTCCCAGGATTTCCTGGAATGTTGGGGCAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGG
ACACCGAGGACCCACAGGAAGACCAGGAAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTCTGGT
CAACCTGGCCGTCCGGGGCCCCCAGGCCCCCCACCTGCAGATTTTTGTGGTCAACAACCAGGAGGAGCTTGAGAGGCTGAACACCCAAAACGCCATTGCC
TTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGACAGCCTTGGCTGGCTCCCCATCCAGCTGACCCCTTTCTACCCTGTGGATTACACTGCAGACCAGC
ACGGCACCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGTGACGACGGTAACAGCGATGTGGGTGACGACTGCATCCGCTGTCACCGTGCCTACTG
TGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCTCTGACTTTGGCTACCTGACATGCGAGACCTATCTCCCTGGGTCATATGGAGACCTG
CAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTACTTCACCTGAGGGCCGTGAGGAGAAGGTGGGCTGCGCCCCACAGAACTGGCAGCAG
CTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCCACCAACCTTTGTGTGACAAAAACAAGGACAAATTCCTGCTACTAAGACATGCTGTT
AACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCTTAATCCAAGGTACTGGAAAACCTTCCACATGCCCAACCTGAGACCTGTTCCCCATC
GCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTCTGGAATGTTGCTGACCCAGGCCGTGGACTTGGCTGAGCACTGTTAGACACACACAG
ACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCTGGAGTACAGGCCTCTCCGCCCCTGACCACCGAAACGTGCAGGCATTCTCACTCACA
CTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAAAGCAGGGAAGTGGGCACGTTCTCTCGGGGTGGCTCACAGCTTTGAACCTGCCAAAG
GACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGTGGCTGCCCAGGAAAAGACTTAACTGTGAAAAAGTACTGAGAACCCACCTGACCCAG
GCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGTGTTTCCCAAAGGGGCGGCTCTTGTGGTTTCAAAATCTCTGGCACCATCTTGACCTC
TTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTCATGTCCATTTCCTGTCCAGGAGACTCATGAGGACTGTGTGACCTGCACAAGCCCAC
ACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATACTCCCCTGAGTCCCCGATCTAGGGCCAGCTGCAGAGGGCTCTCTCTAGGCAGAGCGT
TCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGACAGCGTCCCGTGGCATTTCTTTCATGTCTGCATATTGCATAGCCTTGTCCTCCTGTG
TGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATGTCTGTGAGCTGCCTTTCATCACCATTTTTCCTGAGTAGGGCTTAGTTTTATTCTGG
AAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAAGTAGAAATTGCCCAATTTTTACCAGCTGGAGGGACACCCTTGGGTTTTTGTACGAA
GCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGCTTGGGGCCTGGTGGACCATCACCTGGTGTCTGTAGGTGGACCCGGTCTCCCACAGG
TGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGGCAGCCCTGTTCCCTTCGCCTCTACTGCGAGGCCTGGGGAGATGTTGTTTTCATGCT
GCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAATAAAGGCAGTTCATTTCCCCA
  • refseq_COLQ.F2 (forward primer)
  • refseq_COLQ.R2 (reverse primer)
  • Alternative donor and acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF*
LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT
FINSVLPISAALPSLDQKKRGGHKACCLLTPPP
PPLFPPP
FFRGGRSPLLSPDMKNLMLELETSQS
PCMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGEL
GRPGRKGRPGPPGVPGMPGPIGWPGPEGPRGEK
GDLGMMGLPGSRGPMGSKGYPGSRGEKGSRGEK
GDLGPKGEKGFPGFPGMLGQKGEMGPKGEPGIAG
HRGPTGRPGKRGKQGQKGDSGVMGPPGKPGPSG
QPGRPGPPGPPPADFCGQQPGGA*EAEHPKRHC
LPQRPEISVLQGQPWLAPHPADPFLPCGLHCRPA
RHLWGWAPAAWGGV*RR*QRCG*RLHPLSPCLL
WRWSPA*GCGGL*RL*LWLPDMRDLSPWVIWRP
AMHPVLLHRLHALPLLHLRAVRRRWAAPHRTGSS
FSTVIKLAMSWPRLHQPLCDKNKDKFLLLRHAV
NSV*LEEFRTTASCLNPRYWKTFHMPNLRPVPH
RYHLAKQPATMLFLFWNVADPGRGLG*ALLDTHR
PAGCFRMF*SLWGIWSTGLSAPDHRNVQAFSLT
LGSPLSGLSRPMNHKAGKWARSLGVAHSFEPAK
GPLDWPQPSPA*RGCGCPGKDLTVKKY*EPT*PR
LAPSRG*RRGSSSQCFPKGRLLWFQNLWHHLDL
LALSALCPLSQKCPSCPFPVQETHEDCVTCTSP
HLGRLLVSPWSLGRYSPESPI*GQLQRALSRQSV
PGQSSTSLPPADP*QRPVAFLSCLHIA*PCPPV
CLSSSLFNKIISRACL*AAFHHHFS*VGLSFIL
ERHLQGEVHPHSRPQVEIAQFLPAGGTPLGFCTK
LFNEPVSWGLSRLELGAWWTITWCL*VDPVSHR
*HQPEGGVFRDRHMGSPVPFASTARPGEMLFSC
CFHHHTGVSGWEIKK*RQFISP

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN
LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR
LSSTAFFQSQQPFPAWIRRSVVATKHAAC*RLLH
HHCSHHH
SSEVAEVRFSPQT*RISCWNWRPRSP
RACKAR*APLGLPAPRVHRGFLARQDQREKRGS
LADQEGRVDLAPQVFLACLGPSVGQALKDPGVKK
VTWV*WACQGQEDQWAPRATLDPEGKRDPEVKR
VTWVPKEKRVSQDFLECWGRKVKWVQKVNLG*Q
DTEDPQEDQENEASRDRKGIVELWAHQASLGLLV
NLAVRGPQAPHLQIFVVNNQEELERLNTQNAIA
FRRDQRSLYFKDSLGWLPIQLTPFYPVDYTADQ
HGTCGDGLLQPGEECDDGNSDVGDDCIRCHRAYC
GDGHRHEGVEDCDGSDFGYLTCETYLPGSYGDL
QCTQYCYIDSTPCRYFT*GP*GEGGLRPTELAA
ASPLSSNWPCPGPVSTNLCVTKTRTNSCY*DMLL
TQSDWKNLGPLHPVLIQGTGKPSTCPT*DLFPI
ATIWPNNRLQCSSSSGMLLTQAVDLAEHC*THT
DLQAVLECFDLFGESGVQASPPLTTETCRHSHSH
WAARCRVSLGL*TTKQGSGHVLSGWLTALNLPK
DPSTGHSPAQPDVDVAAQEKT*L*KSTENPPDP
GLPQAEAREEAPLLSVSQRGGSCGFKISGTILTS
WLSLHFAPCLKNVPHVHFLSRRLMRTV*PAQAH
TWAGCWCLLGP*ADTPLSPRSRASCRGLSLGRA
FLARALPLCLLLTPDSVPWHFFHVCILHSLVLLC
A*APPFSIRLLVVHVCELPFITIFPE*GLVLFW
KDISKVRSTPTADLK*KLPNFYQLEGHPWVFVR
SYLMSLCLGDSAGWSLGPGGPSPGVCRWTRSPTG
DINLRVASLETGTWAALFPSPLLRGLGRCCFHA
ASTITLGFLDGK*KNKGSSFP

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT
*PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD
FHQQRSSNLSSPSQPGSEEAWWPQSMLPADASS
TTTVPTT
ILQRWPKSASLPRHEESHAGTGDLAVP
VHARLARLPWASRPPGSTGASWQDRTKGRKGGA
WPTRKEG*TWPPRCSWHAWAHRLARP*RTQG*K
R*PGYDGLARVKRTNGLQGLPWIQRGKGIQR*KG
*PGSQRRKGFPRISWNVGAER*NGSKR*TWDSR
TPRTHRKTRKTRQAGTERG*WSYGPTRQAWAFW
STWPSGAPRPPTCRFLWSTTRRSLRG*TPKTPLP
SAETRDLCTSRTALAGSPSS*PLSTLWITLQTS
TAPVGMGSCSLGRSVTTVTAMWVTTASAVTVPT
VEMVTGMRVWRTVTALTLAT*HARPISLGHMETC
NAPSTATSTPRPAATSPEGREEKVGCAPQNWQQ
LLHCHQTGHVLAPSPPTFV*QKQGQIPATKTCC
*LSLTGRI*DHCILS*SKVLENLPHAQPETCSPS
LPFGQTTGYNALPLLECC*PRPWTWLSTVRHTQ
TCRLF*NVLISLGNLEYRPLRP*PPKRAGILTH
TGQPAVGSL*AYEPQSREVGTFSRGGSQL*TCQR
TPRLATAQPSLTWMWLPRKRLNCEKVLRTHLTQ
ACPKQRLEKRLLFSVFPKGAALVVSKSLAPS*P
LGSLCTLPPVSKMSLMSISCPGDS*GLCDLHKPT
PGQAVGVSLVLRQILP*VPDLGPAAEGSL*AER
SWPELYLFASC*PLTASRGISFMSAYCIALSSC
VPELLPFQ*DY*SCMSVSCLSSPFFLSRA*FYSG
KTSPR*GPPPQQTSSRNCPIFTSWRDTLGFLYE
AI**ACVLGTQQAGAWGLVDHHLVSVGGPGLPQ
VTST*GWRL*RQAHGQPCSLRLYCEAWGDVVFML
LPPSHWGFWMGNKKIKAVHFP

Sequence NM_080544

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC
TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC
TTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGGCCACAAAGCATGCTGCCTGCTGACGCCTCCTCCA
CCACCACTGTTCCCACCACCA
TTCTTCAGAGGTGGCCGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCC
CGTGCATGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGGGAGCT
TGGCCGACCAGGAAGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAA
GGTGACCTGGGTATGATGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGG
GTGACCTGGGTCCCAAAGGAGAAAAGGGTTTCCCAGGATTTCCTGGAATGTTGGGGCAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGG
ACACCGAGGACCCACAGGAAGACCAGGAAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTCTGGT
CAACCTGGCCGTCCGGGGCCCCCAGGCCCCCCACCTGCAGGTCATATGGAGACCTGCAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTA
CTTCACCTGAGGGCCGTGAGGAGAAGGTGGGCTGCGCCCCACAGAACTGGCAGCAGCTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCC
ACCAACCTTTGTGTGACAAAAACAAGGACAAATTCCTGCTACTAAGACATGCTGTTAACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCT
TAATCCAAGGTACTGGAAAACCTTCCACATGCCCAACCTGAGACCTGTTCCCCATCGCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTC
TGGAATGTTGCTGACCCAGGCCGTGGACTTGGCTGAGCACTGTTAGACACACACAGACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCT
GGAGTACAGGCCTCTCCGCCCCTGACCACCGAAACGTGCAGGCATTCTCACTCACACTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAA
AGCAGGGAAGTGGGCACGTTCTCTCGGGGTGGCTCACAGCTTTGAACCTGCCAAAGGACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGT
GGCTGCCCAGGAAAAGACTTAACTGTGAAAAAGTACTGAGAACCCACCTGACCCAGGCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGT
GTTTCCCAAAGGGGCGGCTCTTGTGGTTTCAAAATCTCTGGCACCATCTTGACCTCTTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTC
ATGTCCATTTCCTGTCCAGGAGACTCATGAGGACTGTGTGACCTGCACAAGCCCACACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATAC
TCCCCTGAGTCCCCGATCTAGGGCCAGCTGCAGAGGGCTCTCTCTAGGCAGAGCGTTCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGAC
AGCGTCCCGTGGCATTTCTTTCATGTCTGCATATTGCATAGCCTTGTCCTCCTGTGTGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATG
TCTGTGAGCTGCCTTTCATCACCATTTTTCCTGAGTAGGGCTTAGTTTTATTCTGGAAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAA
GTAGAAATTGCCCAATTTTTACCAGCTGGAGGGACACCCTTGGGTTTTTGTACGAAGCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGC
TTGGGGCCTGGTGGACCATCACCTGGTGTCTGTAGGTGGACCCGGTCTCCCACAGGTGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGG
CAGCCCTGTTCCCTTCGCCTCTACTGCGAGGCCTGGGGAGATGTTGTTTTCATGCTGCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAA
TAAAGGCAGTTCATTTCCCCA
  • refseq_COLQ.F2 (forward primer)
  • refseq_COLQ.R2 (reverse primer)
  • Alternative donor and acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF*
LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT
FINSVLPISAALPSLDQKKRGGHKACCLLTPPP
PPLFPPP
FFRGGRSPLLSPDMKNLMLELETSQS
PCMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGEL
GRPGRKGRPGPPGVPGMPGPIGWPGPEGPRGEK
GDLGMMGLPGSRGPMGSKGYPGSRGEKGSRGEK
GDLGPKGEKGFPGFPGMLGQKGEMGPKGEPGIAG
HRGPTGRPGKRGKQGQKGDSGVMGPPGKPGPSG
QPGRPGPPGPPPAGHMETCNAPSTATSTPRPAA
TSPEGREEKVGCAPQNWQQLLHCHQTGHVLAPSP
PTFV*QKQGQIPATKTCC*LSLTGRI*DHCILS
*SKVLENLPHAQPETCSPSLPFGQTTGYNALPL
LECC*PRPWTWLSTVRHTQTCRLF*NVLISLGNL
EYRPLRP*PPKRAGILTHTGQPAVGSL*AYEPQ
SREVGTFSRGGSQL*TCQRTPRLATAQPSLTWM
WLPRKRLNCEKVLRTHLTQACPKQRLEKRLLFSV
FPKGAALVVSKSLAPS*PLGSLCTLPPVSKMSL
MSISCPGDS*GLCDLHKPTPGQAVGVSLVLRQI
LP*VPDLGPAAEGSL*AERSWPELYLFASC*PLT
ASRGISFMSAYCIALSSCVPELLPFQ*DY*SCM
SVSCLSSPFFLSRA*FYSGKTSPR*GPPPQQTS
SRNCPIFTSWRDTLGFLYEAI**ACVLGTQQAGA
WGLVDHHLVSVGGPGLPQVTST*GWRL*RQAHG
QPCSLRLYCEAWGDVVFMLLPPSHWGFWMGNKK
IKAVHFP

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN
LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR
LSSTAFFQSQQPFPAWIRRSVVATKHAAC*RLLH
HHCSHHH
SSEVAEVRFSPQT*RISCWNWRPRSP
RACKAR*APLGLPAPRVHRGFLARQDQREKRGS
LADQEGRVDLAPQVFLACLGPSVGQALKDPGVKK
VTWV*WACQGQEDQWAPRATLDPEGKRDPEVKR
VTWVPKEKRVSQDFLECWGRKVKWVQKVNLG*Q
DTEDPQEDQENEASRDRKGIVELWAHQASLGLLV
NLAVRGPQAPHLQVIWRPAMHPVLLHRLHALPL
LHLRAVRRRWAAPHRTGSSFSTVIKLAMSWPRL
HQPLCDKNKDKFLLLRHAVNSV*LEEFRTTASCL
NPRYWKTFHMPNLRPVPHRYHLAKQPATMLFLF
WNVADPGRGLG*ALLDTHRPAGCFRMF*SLWGI
WSTGLSAPDHRNVQAFSLTLGSPLSGLSRPMNHK
AGKWARSLGVAHSFEPAKGPLDWPQPSPA*RGC
GCPGKDLTVKKY*EPT*PRLAPSRG*RRGSSSQ
CFPKGRLLWFQNLWHHLDLLALSALCPLSQKCPS
CPFPVQETHEDCVTCTSPHLGRLLVSPWSLGRY
SPESPI*GQLQRALSRQSVPGQSSTSLPPADP*
QRPVAFLSCLHIA*PCPPVCLSSSLFNKIISRAC
L*AAFHHHFS*VGLSFILERHLQGEVHPHSRPQ
VEIAQFLPAGGTPLGFCTKLFNEPVSWGLSRLE
LGAWWTITWCL*VDPVSHR*HQPEGGVFRDRHMG
SPVPFASTARPGEMLFSCCFHHHTGVSGWEIKK
*RQFISP

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT
*PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD
FHQQRSSNLSSPSQPGSEEAWWPQSMLPADASS
TTTVPTT
ILQRWPKSASLPRHEESHAGTGDLAVP
VHARLARLPWASRPPGSTGASWQDRTKGRKGGA
WPTRKEG*TWPPRCSWHAWAHRLARP*RTQG*K
R*PGYDGLARVKRTNGLQGLPWIQRGKGIQR*KG
*PGSQRRKGFPRISWNVGAER*NGSKR*TWDSR
TPRTHRKTRKTRQAGTERG*WSYGPTRQAWAFW
STWPSGAPRPPTCRSYGDLQCTQYCYIDSTPCRY
FT*GP*GEGGLRPTELAAASPLSSNWPCPGPVS
TNLCVTKTRTNSCY*DMLLTQSDWKNLGPLHPV
LIQGTGKPSTCPT*DLFPIATIWPNNRLQCSSSS
GMLLTQAVDLAEHC*THTDLQAVLECFDLFGES
GVQASPPLTTETCRHSHSHWAARCRVSLGL*TT
KQGSGHVLSGWLTALNLPKDPSTGHSPAQPDVDV
AAQEKT*L*KSTENPPDPGLPQAEAREEAPLLS
VSQRGGSCGFKISGTILTSWLSLHFAPCLKNVP
HVHFLSRRLMRTV*PAQAHTWAGCWCLLGP*ADT
PLSPRSRASCRGLSLGRAFLARALPLCLLLTPD
SVPWHFFHVCILHSLVLLCA*APPFSIRLLVVH
VCELPFITIFPE*GLVLFWKDISKVRSTPTADLK
*KLPNFYQLEGHPWVFVRSYLMSLCLGDSAGWS
LGPGGPSPGVCRWTRSPTGDINLRVASLETGTW
AALFPSPLLRGLGRCCFHAASTITLGFLDGK*KN
KGSSFP

Sequence NM_080541

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC
TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC
TTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGGCCACAAAGCATGCTGCCTGCTGACGCCTCCTCCA
CCACCACTGTTCCCACCACCA
TTCTTCAGAGGTGGCCGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCC
CGTGCATGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGTAGACC
TGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAAGGTGACCTGGGTATGATGGGCTTGCCA
GGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGGGTGACCTGGGTCCCAAAGGAGAAAAGG
GTTTCCCAGGATTTCCTGGAATGTTGGGGCAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGGACACCGAGGACCCACAGGAAGACCAGG
AAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTCTGGTCAACCTGGCCGTCCGGGGCCCCCAGGC
CCCCCACCTGCAGGACAACTTATAATGGGACCCAAAGGGGAAAGAGGATTTCCCGGGCCTCCAGGAAGATGTCTTTGTGGACCCACTATGAATGTGAATA
ACCCTTCCTACGGGGAATCTGTGTATGGGCCCAGTTCCCCGCGAGTTCCTGTGATTTTTGTGGTCAACAACCAGGAGGAGCTTGAGAGGCTGAACACCCA
AAACGCCATTGCCTTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGACAGCCTTGGCTGGCTCCCCATCCAGCTGACCCCTTTCTACCCTGTGGATTAC
ACTGCAGACCAGCACGGCACCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGTGACGACGGTAACAGCGATGTGGGTGACGACTGCATCCGCTGTC
ACCGTGCCTACTGTGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCTCTGACTTTGGCTACCTGACATGCGAGACCTATCTCCCTGGGTC
ATATGGAGACCTGCAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTACTTCACCTGAGGGCCGTGAGGAGAAGGTGGGCTGCGCCCCACA
GAACTGGCAGCAGCTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCCACCAACCTTTGTGTGACAAAAACAAGGACAAATTCCTGCTACT
AAGACATGCTGTTAACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCTTAATCCAAGGTACTGGAAAACCTTCCACATGCCCAACCTGAGA
CCTGTTCCCCATCGCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTCTGGAATGTTGCTGACCCAGGCCGTGGACTTGGCTGAGCACTGT
TAGACACACACAGACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCTGGAGTACAGGCCTCTCCGCCCCTGACCACCGAAACGTGCAGGC
ATTCTCACTCACACTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAAAGCAGGGAAGTGGGCACGTTCTCTCGGGGTGGCTCACAGCTTT
GAACCTGCCAAAGGACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGTGGCTGCCCAGGAAAAGACTTAACTGTGAAAAAGTACTGAGAAC
CCACCTGACCCAGGCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGTGTTTCCCAAAGGGGCGGCTCTTGTGGTTTCAAAATCTCTGGCA
CCATCTTGACCTCTTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTCATGTCCATTTCCTGTCCAGGAGACTCATGAGGACTGTGTGACC
TGCACAAGCCCACACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATACTCCCCTGAGTCCCCGATCTAGGGCCAGCTGCAGAGGGCTCTCT
CTAGGCAGAGCGTTCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGACAGCGTCCCGTGGCATTTCTTTCATGTCTGCATATTGCATAGCC
TTGTCCTCCTGTGTGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATGTCTGTGAGCTGCCTTTCATCACCATTTTTCCTGAGTAGGGCTT
AGTTTTATTCTGGAAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAAGTAGAAATTGCCCAATTTTTACCAGCTGGAGGGACACCCTTGG
GTTTTTGTACGAAGCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGCTTGGGGCCTGGTGGACCATCACCTGGTGTCTGTAGGTGGACCC
GGTCTCCCACAGGTGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGGCAGCCCTGTTCCCTTCGCCTCTACTGCGAGGCCTGGGGAGATG
TTGTTTTCATGCTGCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAATAAAGGCAGTTCATTTCCCCA
  • refseq_COLQ.F2 (forward primer)
  • refseq_COLQ.R2 (reverse primer)
  • Alternative donor and acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF*
LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT
FINSVLPISAALPSLDQKKRGGHKACCLLTPPP
PPLFPPP
FFRGGRSPLLSPDMKNLMLELETSQS
PCMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGRP
GPPGVPGMPGPIGWPGPEGPRGEKGDLGMMGLP
GSRGPMGSKGYPGSRGEKGSRGEKGDLGPKGEK
GFPGFPGMLGQKGEMGPKGEPGIAGHRGPTGRPG
KRGKQGQKGDSGVMGPPGKPGPSGQPGRPGPPG
PPPAGQLIMGPKGERGFPGPPGRCLCGPTMNVN
NPSYGESVYGPSSPRVPVIFVVNNQEELERLNTQ
NAIAFRRDQRSLYFKDSLGWLPIQLTPFYPVDY
TADQHGTCGDGLLQPGEECDDGNSDVGDDCIRC
HRAYCGDGHRHEGVEDCDGSDFGYLTCETYLPGS
YGDLQCTQYCYIDSTPCRYFT*GP*GEGGLRPT
ELAAASPLSSNWPCPGPVSTNLCVTKTRTNSCY
*DMLLTQSDWKNLGPLHPVLIQGTGKPSTCPT*D
LFPIATIWPNNRLQCSSSSGMLLTQAVDLAEHC
*THTDLQAVLECFDLFGESGVQASPPLTTETCR
HSHSHWAARCRVSLGL*TTKQGSGHVLSGWLTAL
NLPKDPSTGHSPAQPDVDVAAQEKT*L*KSTEN
PPDPGLPQAEAREEAPLLSVSQRGGSCGFKISG
TILTSWLSLHFAPCLKNVPHVHFLSRRLMRTV*P
AQAHTWAGCWCLLGP*ADTPLSPRSRASCRGLS
LGRAFLARALPLCLLLTPDSVPWHFFHVCILHS
LVLLCA*APPFSIRLLVVHVCELPFITIFPE*GL
VLFWKDISKVRSTPTADLK*KLPNFYQLEGHPW
VFVRSYLMSLCLGDSAGWSLGPGGPSPGVCRWT
RSPTGDINLRVASLETGTWAALFPSPLLRGLGRC
CFHAASTITLGFLDGK*KNKGSSFP

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN
LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR
LSSTAFFQSQQPFPAWIRRSVVATKHAAC*RLLH
HHCSHHH
SSEVAEVRFSPQT*RISCWNWRPRSP
RACKAR*APLGLPAPRVHRGFLARQDQREKRVD
LAPQVFLACLGPSVGQALKDPGVKKVTWV*WACQ
GQEDQWAPRATLDPEGKRDPEVKRVTWVPKEKR
VSQDFLECWGRKVKWVQKVNLG*QDTEDPQEDQ
ENEASRDRKGIVELWAHQASLGLLVNLAVRGPQA
PHLQDNL*WDPKGKEDFPGLQEDVFVDPL*M*I
TLPTGNLCMGPVPREFL*FLWSTTRRSLRG*TP
KTPLPSAETRDLCTSRTALAGSPSS*PLSTLWIT
LQTSTAPVGMGSCSLGRSVTTVTAMWVTTASAV
TVPTVEMVTGMRVWRTVTALTLAT*HARPISLG
HMETCNAPSTATSTPRPAATSPEGREEKVGCAPQ
NWQQLLHCHQTGHVLAPSPPTFV*QKQGQIPAT
KTCC*LSLTGRI*DHCILS*SKVLENLPHAQPE
TCSPSLPFGQTTGYNALPLLECC*PRPWTWLSTV
RHTQTCRLF*NVLISLGNLEYRPLRP*PPKRAG
ILTHTGQPAVGSL*AYEPQSREVGTFSRGGSQL
*TCQRTPRLATAQPSLTWMWLPRKRLNCEKVLRT
HLTQACPKQRLEKRLLFSVFPKGAALVVSKSLA
PS*PLGSLCTLPPVSKMSLMSISCPGDS*GLCD
LHKPTPGQAVGVSLVLRQILP*VPDLGPAAEGSL
*AERSWPELYLFASC*PLTASRGISFMSAYCIA
LSSCVPELLPFQ*DY*SCMSVSCLSSPFFLSRA
*FYSGKTSPR*GPPPQQTSSRNCPIFTSWRDTLG
FLYEAI**ACVLGTQQAGAWGLVDHHLVSVGGP
GLPQVTST*GWRL*RQAHGQPCSLRLYCEAWGD
VVFMLLPPSHWGFWMGNKKIKAVHFP

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT
*PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD
FHQQRSSNLSSPSQPGSEEAWWPQSMLPADASS
TTTVPTT
ILQRWPKSASLPRHEESHAGTGDLAVP
VHARLARLPWASRPPGSTGASWQDRTKGRKG*T
WPPRCSWHAWAHRLARP*RTQG*KR*PGYDGLA
RVKRTNGLQGLPWIQRGKGIQR*KG*PGSQRRKG
FPRISWNVGAER*NGSKR*TWDSRTPRTHRKTR
KTRQAGTERG*WSYGPTRQAWAFWSTWPSGAPR
PPTCRTTYNGTQRGKRISRASRKMSLWTHYECE*
PFLRGICVWAQFPASSCDFCGQQPGGA*EAEHP
KRHCLPQRPEISVLQGQPWLAPHPADPFLPCGL
HCRPARHLWGWAPAAWGGV*RR*QRCG*RLHPLS
PCLLWRWSPA*GCGGL*RL*LWLPDMRDLSPWV
IWRPAMHPVLLHRLHALPLLHLRAVRRRWAAPH
RTGSSFSTVIKLAMSWPRLHQPLCDKNKDKFLLL
RHAVNSV*LEEFRTTASCLNPRYWKTFHMPNLR
PVPHRYHLAKQPATMLFLFWNVADPGRGLG*AL
LDTHRPAGCFRMF*SLWGIWSTGLSAPDHRNVQA
FSLTLGSPLSGLSRPMNHKAGKWARSLGVAHSF
EPAKGPLDWPQPSPA*RGCGCPGKDLTVKKY*E
PT*PRLAPSRG*RRGSSSQCFPKGRLLWFQNLWH
HLDLLALSALCPLSQKCPSCPFPVQETHEDCVT
CTSPHLGRLLVSPWSLGRYSPESPI*GQLQRAL
SRQSVPGQSSTSLPPADP*QRPVAFLSCLHIA*P
CPPVCLSSSLFNKIISRACL*AAFHHHFS*VGL
SFILERHLQGEVHPHSRPQVEIAQFLPAGGTPL
GFCTKLFNEPVSWGLSRLELGAWWTITWCL*VDP
VSHR*HQPEGGVFRDRHMGSPVPFASTARPGEM
LFSCCFHHHTGVSGWEIKK*RQFISP

Sequence NM_080540

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC
TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC
TTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGGCCACAAAGCATGCTGCCTGCTGACGCCTCCTCCA
CCACCACTGTTCCCACCACCA
TTCTTCAGAGGTGGCCGAAGTCCGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCCCGTGCATGCAAGG
CTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGGGAGCTTGGCCGACCAGGA
AGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAAGGTGACCTGGGTA
TGATGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGGGTGACCTGGGTCC
CAAAGGAGAAAAGGGTTTCCCAGGATTTCCTGGAATGTTGGGGCAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGGACACCGAGGACCC
ACAGGAAGACCAGGAAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTCTGGTCAACCTGGCCGTC
CGGGGCCCCCAGGCCCCCCACCTGCAGGACAACTTATAATGGGACCCAAAGGGGAAAGAGGATTTCCCGGGCCTCCAGGAAGATGTCTTTGTGGACCCAC
TATGAATGTGAATAACCCTTCCTACGGGGAATCTGTGTATGGGCCCAGTTCCCCGCGAGTTCCTGTGATTTTTGTGGTCAACAACCAGGAGGAGCTTGAG
AGGCTGAACACCCAAAACGCCATTGCCTTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGACAGCCTTGGCTGGCTCCCCATCCAGCTGACCCCTTTCT
ACCCTGTGGATTACACTGCAGACCAGCACGGCACCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGTGACGACGGTAACAGCGATGTGGGTGACGA
CTGCATCCGCTGTCACCGTGCCTACTGTGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCTCTGACTTTGGCTACCTGACATGCGAGACC
TATCTCCCTGGGTCATATGGAGACCTGCAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTACTTCACCTGAGGGCCGTGAGGAGAAGGTG
GGCTGCGCCCCACAGAACTGGCAGCAGCTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCCACCAACCTTTGTGTGACAAAAACAAGGAC
AAATTCCTGCTACTAAGACATGCTGTTAACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCTTAATCCAAGGTACTGGAAAACCTTCCACA
TGCCCAACCTGAGACCTGTTCCCCATCGCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTCTGGAATGTTGCTGACCCAGGCCGTGGACT
TGGCTGAGCACTGTTAGACACACACAGACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCTGGAGTACAGGCCTCTCCGCCCCTGACCAC
CGAAACGTGCAGGCATTCTCACTCACACTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAAAGCAGGGAAGTGGGCACGTTCTCTCGGGG
TGGCTCACAGCTTTGAACCTGCCAAAGGACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGTGGCTGCCCAGGAAAAGACTTAACTGTGAA
AAAGTACTGAGAACCCACCTGACCCAGGCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGTGTTTCCCAAAGGGGCGGCTCTTGTGGTTT
CAAAATCTCTGGCACCATCTTGACCTCTTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTCATGTCCATTTCCTGTCCAGGAGACTCATG
AGGACTGTGTGACCTGCACAAGCCCACACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATACTCCCCTGAGTCCCCGATCTAGGGCCAGCT
GCAGAGGGCTCTCTCTAGGCAGAGCGTTCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGACAGCGTCCCGTGGCATTTCTTTCATGTCTG
CATATTGCATAGCCTTGTCCTCCTGTGTGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATGTCTGTGAGCTGCCTTTCATCACCATTTTT
CCTGAGTAGGGCTTAGTTTTATTCTGGAAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAAGTAGAAATTGCCCAATTTTTACCAGCTGG
AGGGACACCCTTGGGTTTTTGTACGAAGCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGCTTGGGGCCTGGTGGACCATCACCTGGTGT
CTGTAGGTGGACCCGGTCTCCCACAGGTGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGGCAGCCCTGTTCCCTTCGCCTCTACTGCGA
GGCCTGGGGAGATGTTGTTTTCATGCTGCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAATAAAGGCAGTTCATTTCCCCA
  • refseq_COLQ.F2 (forward primer)
  • refseq_COLQ.R2 (reverse primer)
  • Alternative donor and acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF*
LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT
FINSVLPISAALPSLDQKKRGGHKACCLLTPPP
PPLFPPP
FFRGGRSPT*RISCWNWRPRSPRACK
AR*APLGLPAPRVHRGFLARQDQREKRGSLADQE
GRVDLAPQVFLACLGPSVGQALKDPGVKKVTWV
*WACQGQEDQWAPRATLDPEGKRDPEVKRVTWV
PKEKRVSQDFLECWGRKVKWVQKVNLG*QDTEDP
QEDQENEASRDRKGIVELWAHQASLGLLVNLAV
RGPQAPHLQDNL*WDPKGKEDFPGLQEDVFVDP
L*M*ITLPTGNLCMGPVPREFL*FLWSTTRRSLR
G*TPKTPLPSAETRDLCTSRTALAGSPSS*PLS
TLWITLQTSTAPVGMGSCSLGRSVTTVTAMWVT
TASAVTVPTVEMVTGMRVWRTVTALTLAT*HARP
ISLGHMETCNAPSTATSTPRPAATSPEGREEKV
GCAPQNWQQLLHCHQTGHVLAPSPPTFV*QKQG
QIPATKTCC*LSLTGRI*DHCILS*SKVLENLPH
AQPETCSPSLPFGQTTGYNALPLLECC*PRPWT
WLSTVRHTQTCRLF*NVLISLGNLEYRPLRP*P
PKRAGILTHTGQPAVGSL*AYEPQSREVGTFSRG
GSQL*TCQRTPRLATAQPSLTWMWLPRKRLNCE
KVLRTHLTQACPKQRLEKRLLFSVFPKGAALVV
SKSLAPS*PLGSLCTLPPVSKMSLMSISCPGDS*
GLCDLHKPTPGQAVGVSLVLRQILP*VPDLGPA
AEGSL*AERSWPELYLFASC*PLTASRGISFMS
AYCIALSSCVPELLPFQ*DY*SCMSVSCLSSPFF
LSRA*FYSGKTSPR*GPPPQQTSSRNCPIFTSW
RDTLGFLYEAI**ACVLGTQQAGAWGLVDHHLV
SVGGPGLPQVTST*GWRL*RQAHGQPCSLRLYCE
AWGDVVFMLLPPSHWGFWMGNKKIKAVHFP

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN
LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR
LSSTAFFQSQQPFPAWIRRSVVATKHAAC*RLLH
HHCSHHH
SSEVAEVRHEESHAGTGDLAVPVHAR
LARLPWASRPPGSTGASWQDRTKGRKGGAWPTR
KEG*TWPPRCSWHAWAHRLARP*RTQG*KR*PGY
DGLARVKRTNGLQGLPWIQRGKGIQR*KG*PGS
QRRKGFPRISWNVGAER*NGSKR*TWDSRTPRT
HRKTRKTRQAGTERG*WSYGPTRQAWAFWSTWPS
GAPRPPTCRTTYNGTQRGKRISRASRKMSLWTH
YECE*PFLRGICVWAQFPASSCDFCGQQPGGA*
EAEHPKRHCLPQRPEISVLQGQPWLAPHPADPFL
PCGLHCRPARHLWGWAPAAWGGV*RR*QRCG*R
LHPLSPCLLWRWSPA*GCGGL*RL*LWLPDMRD
LSPWVIWRPAMHPVLLHRLHALPLLHLRAVRRRW
AAPHRTGSSFSTVIKLAMSWPRLHQPLCDKNKD
KFLLLRHAVNSV*LEEFRTTASCLNPRYWKTFH
MPNLRPVPHRYHLAKQPATMLFLFWNVADPGRGL
G*ALLDTHRPAGCFRMF*SLWGIWSTGLSAPDH
RNVQAFSLTLGSPLSGLSRPMNHKAGKWARSLG
VAHSFEPAKGPLDWPQPSPA*RGCGCPGKDLTVK
KY*EPT*PRLAPSRG*RRGSSSQCFPKGRLLWF
QNLWHHLDLLALSALCPLSQKCPSCPFPVQETH
EDCVTCTSPHLGRLLVSPWSLGRYSPESPI*GQL
QRALSRQSVPGQSSTSLPPADP*QRPVAFLSCL
HIA*PCPPVCLSSSLFNKIISRACL*AAFHHHF
S*VGLSFILERHLQGEVHPHSRPQVEIAQFLPAG
GTPLGFCTKLFNEPVSWGLSRLELGAWWTITWC
L*VDPVSHR*HQPEGGVFRDRHMGSPVPFASTA
RPGEMLFSCCFHHHTGVSGWEIKK*RQFISP

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT
*PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD
FHQQRSSNLSSPSQPGSEEAWWPQSMLPADASS
TTTVPTT
ILQRWPKSDMKNLMLELETSQSPCMQG
SLGSPGPPGPQGPPGLPGKTGPKGEKGELGRPG
RKGRPGPPGVPGMPGPIGWPGPEGPRGEKGDLG
MMGLPGSRGPMGSKGYPGSRGEKGSRGEKGDLGP
KGEKGFPGFPGMLGQKGEMGPKGEPGIAGHRGP
TGRPGKRGKQGQKGDSGVMGPPGKPGPSGQPGR
PGPPGPPPAGQLIMGPKGERGFPGPPGRCLCGPT
MNVNNPSYGESVYGPSSPRVPVIFVVNNQEELE
RLNTQNAIAFRRDQRSLYFKDSLGWLPIQLTPF
YPVDYTADQHGTCGDGLLQPGEECDDGNSDVGDD
CIRCHRAYCGDGHRHEGVEDCDGSDFGYLTCET
YLPGSYGDLQCTQYCYIDSTPCRYFT*GP*GEG
GLRPTELAAASPLSSNWPCPGPVSTNLCVTKTRT
NSCY*DMLLTQSDWKNLGPLHPVLIQGTGKPST
CPT*DLFPIATIWPNNRLQCSSSSGMLLTQAVD
LAEHC*THTDLQAVLECFDLFGESGVQASPPLTT
ETCRHSHSHWAARCRVSLGL*TTKQGSGHVLSG
WLTALNLPKDPSTGHSPAQPDVDVAAQEKT*L*
KSTENPPDPGLPQAEAREEAPLLSVSQRGGSCGF
KISGTILTSWLSLHFAPCLKNVPHVHFLSRRLM
RTV*PAQAHTWAGCWCLLGP*ADTPLSPRSRAS
CRGLSLGRAFLARALPLCLLLTPDSVPWHFFHVC
ILHSLVLLCA*APPFSIRLLVVHVCELPFITIF
PE*GLVLFWKDISKVRSTPTADLK*KLPNFYQL
EGHPWVFVRSYLMSLCLGDSAGWSLGPGGPSPGV
CRWTRSPTGDINLRVASLETGTWAALFPSPLLR
GLGRCCFHAASTITLGFLDGK*KNKGSSFP

Copyright ©2008,2017, all rights reserved.