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Sequences

Sequence NM_080540

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC TTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGGCCACAAAGCATGCTGCCTGCTGACGCCTCCTCCA CCACCACTGTTCCCACCACCATTCTTCAGAGGTGGCCGAAGTCCGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCCCGTGCATGCAAGG CTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGGGAGCTTGGCCGACCAGGA AGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAAGGTGACCTGGGTA TGATGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGGGTGACCTGGGTCC CAAAGGAGAAAAGGGTTTCCCAGGATTTCCTGGAATGTTGGGGCAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGGACACCGAGGACCC ACAGGAAGACCAGGAAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTCTGGTCAACCTGGCCGTC CGGGGCCCCCAGGCCCCCCACCTGCAGGACAACTTATAATGGGACCCAAAGGGGAAAGAGGATTTCCCGGGCCTCCAGGAAGATGTCTTTGTGGACCCAC TATGAATGTGAATAACCCTTCCTACGGGGAATCTGTGTATGGGCCCAGTTCCCCGCGAGTTCCTGTGATTTTTGTGGTCAACAACCAGGAGGAGCTTGAG AGGCTGAACACCCAAAACGCCATTGCCTTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGACAGCCTTGGCTGGCTCCCCATCCAGCTGACCCCTTTCT ACCCTGTGGATTACACTGCAGACCAGCACGGCACCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGTGACGACGGTAACAGCGATGTGGGTGACGA CTGCATCCGCTGTCACCGTGCCTACTGTGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCTCTGACTTTGGCTACCTGACATGCGAGACC TATCTCCCTGGGTCATATGGAGACCTGCAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTACTTCACCTGAGGGCCGTGAGGAGAAGGTG GGCTGCGCCCCACAGAACTGGCAGCAGCTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCCACCAACCTTTGTGTGACAAAAACAAGGAC AAATTCCTGCTACTAAGACATGCTGTTAACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCTTAATCCAAGGTACTGGAAAACCTTCCACA TGCCCAACCTGAGACCTGTTCCCCATCGCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTCTGGAATGTTGCTGACCCAGGCCGTGGACT TGGCTGAGCACTGTTAGACACACACAGACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCTGGAGTACAGGCCTCTCCGCCCCTGACCAC CGAAACGTGCAGGCATTCTCACTCACACTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAAAGCAGGGAAGTGGGCACGTTCTCTCGGGG TGGCTCACAGCTTTGAACCTGCCAAAGGACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGTGGCTGCCCAGGAAAAGACTTAACTGTGAA AAAGTACTGAGAACCCACCTGACCCAGGCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGTGTTTCCCAAAGGGGCGGCTCTTGTGGTTT CAAAATCTCTGGCACCATCTTGACCTCTTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTCATGTCCATTTCCTGTCCAGGAGACTCATG AGGACTGTGTGACCTGCACAAGCCCACACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATACTCCCCTGAGTCCCCGATCTAGGGCCAGCT GCAGAGGGCTCTCTCTAGGCAGAGCGTTCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGACAGCGTCCCGTGGCATTTCTTTCATGTCTG CATATTGCATAGCCTTGTCCTCCTGTGTGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATGTCTGTGAGCTGCCTTTCATCACCATTTTT CCTGAGTAGGGCTTAGTTTTATTCTGGAAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAAGTAGAAATTGCCCAATTTTTACCAGCTGG AGGGACACCCTTGGGTTTTTGTACGAAGCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGCTTGGGGCCTGGTGGACCATCACCTGGTGT CTGTAGGTGGACCCGGTCTCCCACAGGTGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGGCAGCCCTGTTCCCTTCGCCTCTACTGCGA GGCCTGGGGAGATGTTGTTTTCATGCTGCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAATAAAGGCAGTTCATTTCCCCA

refseq_COLQ.F2 (forward primer)
refseq_COLQ.R2 (reverse primer)
Alternative acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF* LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT FINSVLPISAALPSLDQKKRGGHKACCLLTPPP PPLFPPPFFRGGRSPT*RISCWNWRPRSPRACK AR*APLGLPAPRVHRGFLARQDQREKRGSLADQE GRVDLAPQVFLACLGPSVGQALKDPGVKKVTWV *WACQGQEDQWAPRATLDPEGKRDPEVKRVTWV PKEKRVSQDFLECWGRKVKWVQKVNLG*QDTEDP QEDQENEASRDRKGIVELWAHQASLGLLVNLAV RGPQAPHLQDNL*WDPKGKEDFPGLQEDVFVDP L*M*ITLPTGNLCMGPVPREFL*FLWSTTRRSLR G*TPKTPLPSAETRDLCTSRTALAGSPSS*PLS TLWITLQTSTAPVGMGSCSLGRSVTTVTAMWVT TASAVTVPTVEMVTGMRVWRTVTALTLAT*HARP ISLGHMETCNAPSTATSTPRPAATSPEGREEKV GCAPQNWQQLLHCHQTGHVLAPSPPTFV*QKQG QIPATKTCC*LSLTGRI*DHCILS*SKVLENLPH AQPETCSPSLPFGQTTGYNALPLLECC*PRPWT WLSTVRHTQTCRLF*NVLISLGNLEYRPLRP*P PKRAGILTHTGQPAVGSL*AYEPQSREVGTFSRG GSQL*TCQRTPRLATAQPSLTWMWLPRKRLNCE KVLRTHLTQACPKQRLEKRLLFSVFPKGAALVV SKSLAPS*PLGSLCTLPPVSKMSLMSISCPGDS* GLCDLHKPTPGQAVGVSLVLRQILP*VPDLGPA AEGSL*AERSWPELYLFASC*PLTASRGISFMS AYCIALSSCVPELLPFQ*DY*SCMSVSCLSSPFF LSRA*FYSGKTSPR*GPPPQQTSSRNCPIFTSW RDTLGFLYEAI**ACVLGTQQAGAWGLVDHHLV SVGGPGLPQVTST*GWRL*RQAHGQPCSLRLYCE AWGDVVFMLLPPSHWGFWMGNKKIKAVHFP

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR LSSTAFFQSQQPFPAWIRRSVVATKHAAC*RLLH HHCSHHHSSEVAEVRHEESHAGTGDLAVPVHAR LARLPWASRPPGSTGASWQDRTKGRKGGAWPTR KEG*TWPPRCSWHAWAHRLARP*RTQG*KR*PGY DGLARVKRTNGLQGLPWIQRGKGIQR*KG*PGS QRRKGFPRISWNVGAER*NGSKR*TWDSRTPRT HRKTRKTRQAGTERG*WSYGPTRQAWAFWSTWPS GAPRPPTCRTTYNGTQRGKRISRASRKMSLWTH YECE*PFLRGICVWAQFPASSCDFCGQQPGGA* EAEHPKRHCLPQRPEISVLQGQPWLAPHPADPFL PCGLHCRPARHLWGWAPAAWGGV*RR*QRCG*R LHPLSPCLLWRWSPA*GCGGL*RL*LWLPDMRD LSPWVIWRPAMHPVLLHRLHALPLLHLRAVRRRW AAPHRTGSSFSTVIKLAMSWPRLHQPLCDKNKD KFLLLRHAVNSV*LEEFRTTASCLNPRYWKTFH MPNLRPVPHRYHLAKQPATMLFLFWNVADPGRGL G*ALLDTHRPAGCFRMF*SLWGIWSTGLSAPDH RNVQAFSLTLGSPLSGLSRPMNHKAGKWARSLG VAHSFEPAKGPLDWPQPSPA*RGCGCPGKDLTVK KY*EPT*PRLAPSRG*RRGSSSQCFPKGRLLWF QNLWHHLDLLALSALCPLSQKCPSCPFPVQETH EDCVTCTSPHLGRLLVSPWSLGRYSPESPI*GQL QRALSRQSVPGQSSTSLPPADP*QRPVAFLSCL HIA*PCPPVCLSSSLFNKIISRACL*AAFHHHF S*VGLSFILERHLQGEVHPHSRPQVEIAQFLPAG GTPLGFCTKLFNEPVSWGLSRLELGAWWTITWC L*VDPVSHR*HQPEGGVFRDRHMGSPVPFASTA RPGEMLFSCCFHHHTGVSGWEIKK*RQFISP

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT *PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD FHQQRSSNLSSPSQPGSEEAWWPQSMLPADASS TTTVPTTILQRWPKSDMKNLMLELETSQSPCMQG SLGSPGPPGPQGPPGLPGKTGPKGEKGELGRPG RKGRPGPPGVPGMPGPIGWPGPEGPRGEKGDLG MMGLPGSRGPMGSKGYPGSRGEKGSRGEKGDLGP KGEKGFPGFPGMLGQKGEMGPKGEPGIAGHRGP TGRPGKRGKQGQKGDSGVMGPPGKPGPSGQPGR PGPPGPPPAGQLIMGPKGERGFPGPPGRCLCGPT MNVNNPSYGESVYGPSSPRVPVIFVVNNQEELE RLNTQNAIAFRRDQRSLYFKDSLGWLPIQLTPF YPVDYTADQHGTCGDGLLQPGEECDDGNSDVGDD CIRCHRAYCGDGHRHEGVEDCDGSDFGYLTCET YLPGSYGDLQCTQYCYIDSTPCRYFT*GP*GEG GLRPTELAAASPLSSNWPCPGPVSTNLCVTKTRT NSCY*DMLLTQSDWKNLGPLHPVLIQGTGKPST CPT*DLFPIATIWPNNRLQCSSSSGMLLTQAVD LAEHC*THTDLQAVLECFDLFGESGVQASPPLTT ETCRHSHSHWAARCRVSLGL*TTKQGSGHVLSG WLTALNLPKDPSTGHSPAQPDVDVAAQEKT*L* KSTENPPDPGLPQAEAREEAPLLSVSQRGGSCGF KISGTILTSWLSLHFAPCLKNVPHVHFLSRRLM RTV*PAQAHTWAGCWCLLGP*ADTPLSPRSRAS CRGLSLGRAFLARALPLCLLLTPDSVPWHFFHVC ILHSLVLLCA*APPFSIRLLVVHVCELPFITIF PE*GLVLFWKDISKVRSTPTADLK*KLPNFYQL EGHPWVFVRSYLMSLCLGDSAGWSLGPGGPSPGV CRWTRSPTGDINLRVASLETGTWAALFPSPLLR GLGRCCFHAASTITLGFLDGK*KNKGSSFP

Sequence NM_080539

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC TTTCATCAACAGCGTTCTTTTCTTCAGAGGTGGCCGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCCCG TGCATGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGGGAGCTTG GCCGACCAGGAAGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAAGG TGACCTGGGTATGATGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGGGT GACCTGGGTCCCAAAGGAGAAAAGGGTTTCCCAGGATTTCCTGGAATGTTGGGGCAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGGAC ACCGAGGACCCACAGGAAGACCAGGAAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTCTGGTCA ACCTGGCCGTCCGGGGCCCCCAGGCCCCCCACCTGCAGGACAACTTATAATGGGACCCAAAGGGGAAAGAGGATTTCCCGGGCCTCCAGGAAGATGTCTT TGTGGACCCACTATGAATGTGAATAACCCTTCCTACGGGGAATCTGTGTATGGGCCCAGTTCCCCGCGAGTTCCTGTGATTTTTGTGGTCAACAACCAGG AGGAGCTTGAGAGGCTGAACACCCAAAACGCCATTGCCTTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGACAGCCTTGGCTGGCTCCCCATCCAGCT GACCCCTTTCTACCCTGTGGATTACACTGCAGACCAGCACGGCACCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGTGACGACGGTAACAGCGAT GTGGGTGACGACTGCATCCGCTGTCACCGTGCCTACTGTGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCTCTGACTTTGGCTACCTGA CATGCGAGACCTATCTCCCTGGGTCATATGGAGACCTGCAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTACTTCACCTGAGGGCCGTG AGGAGAAGGTGGGCTGCGCCCCACAGAACTGGCAGCAGCTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCCACCAACCTTTGTGTGACA AAAACAAGGACAAATTCCTGCTACTAAGACATGCTGTTAACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCTTAATCCAAGGTACTGGAA AACCTTCCACATGCCCAACCTGAGACCTGTTCCCCATCGCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTCTGGAATGTTGCTGACCCA GGCCGTGGACTTGGCTGAGCACTGTTAGACACACACAGACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCTGGAGTACAGGCCTCTCCG CCCCTGACCACCGAAACGTGCAGGCATTCTCACTCACACTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAAAGCAGGGAAGTGGGCACG TTCTCTCGGGGTGGCTCACAGCTTTGAACCTGCCAAAGGACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGTGGCTGCCCAGGAAAAGAC TTAACTGTGAAAAAGTACTGAGAACCCACCTGACCCAGGCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGTGTTTCCCAAAGGGGCGGC TCTTGTGGTTTCAAAATCTCTGGCACCATCTTGACCTCTTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTCATGTCCATTTCCTGTCCA GGAGACTCATGAGGACTGTGTGACCTGCACAAGCCCACACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATACTCCCCTGAGTCCCCGATC TAGGGCCAGCTGCAGAGGGCTCTCTCTAGGCAGAGCGTTCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGACAGCGTCCCGTGGCATTTC TTTCATGTCTGCATATTGCATAGCCTTGTCCTCCTGTGTGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATGTCTGTGAGCTGCCTTTCA TCACCATTTTTCCTGAGTAGGGCTTAGTTTTATTCTGGAAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAAGTAGAAATTGCCCAATTT TTACCAGCTGGAGGGACACCCTTGGGTTTTTGTACGAAGCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGCTTGGGGCCTGGTGGACCA TCACCTGGTGTCTGTAGGTGGACCCGGTCTCCCACAGGTGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGGCAGCCCTGTTCCCTTCGC CTCTACTGCGAGGCCTGGGGAGATGTTGTTTTCATGCTGCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAATAAAGGCAGTTCATTTCC CCA

refseq_COLQ.F2 (forward primer)
refseq_COLQ.R2 (reverse primer)
Alternative acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF* LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT FINSVLFFRGGRSPLLSPDMKNLMLELETSQSP CMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGEL GRPGRKGRPGPPGVPGMPGPIGWPGPEGPRGEKG DLGMMGLPGSRGPMGSKGYPGSRGEKGSRGEKG DLGPKGEKGFPGFPGMLGQKGEMGPKGEPGIAG HRGPTGRPGKRGKQGQKGDSGVMGPPGKPGPSGQ PGRPGPPGPPPAGQLIMGPKGERGFPGPPGRCL CGPTMNVNNPSYGESVYGPSSPRVPVIFVVNNQ EELERLNTQNAIAFRRDQRSLYFKDSLGWLPIQL TPFYPVDYTADQHGTCGDGLLQPGEECDDGNSD VGDDCIRCHRAYCGDGHRHEGVEDCDGSDFGYL TCETYLPGSYGDLQCTQYCYIDSTPCRYFT*GP* GEGGLRPTELAAASPLSSNWPCPGPVSTNLCVT KTRTNSCY*DMLLTQSDWKNLGPLHPVLIQGTG KPSTCPT*DLFPIATIWPNNRLQCSSSSGMLLTQ AVDLAEHC*THTDLQAVLECFDLFGESGVQASP PLTTETCRHSHSHWAARCRVSLGL*TTKQGSGH VLSGWLTALNLPKDPSTGHSPAQPDVDVAAQEKT *L*KSTENPPDPGLPQAEAREEAPLLSVSQRGG SCGFKISGTILTSWLSLHFAPCLKNVPHVHFLS RRLMRTV*PAQAHTWAGCWCLLGP*ADTPLSPRS RASCRGLSLGRAFLARALPLCLLLTPDSVPWHF FHVCILHSLVLLCA*APPFSIRLLVVHVCELPF ITIFPE*GLVLFWKDISKVRSTPTADLK*KLPNF YQLEGHPWVFVRSYLMSLCLGDSAGWSLGPGGP SPGVCRWTRSPTGDINLRVASLETGTWAALFPS PLLRGLGRCCFHAASTITLGFLDGK*KNKGSSFP

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR LSSTAFFSSEVAEVRFSPQT*RISCWNWRPRSPR ACKAR*APLGLPAPRVHRGFLARQDQREKRGSL ADQEGRVDLAPQVFLACLGPSVGQALKDPGVKK VTWV*WACQGQEDQWAPRATLDPEGKRDPEVKRV TWVPKEKRVSQDFLECWGRKVKWVQKVNLG*QD TEDPQEDQENEASRDRKGIVELWAHQASLGLLV NLAVRGPQAPHLQDNL*WDPKGKEDFPGLQEDVF VDPL*M*ITLPTGNLCMGPVPREFL*FLWSTTR RSLRG*TPKTPLPSAETRDLCTSRTALAGSPSS *PLSTLWITLQTSTAPVGMGSCSLGRSVTTVTAM WVTTASAVTVPTVEMVTGMRVWRTVTALTLAT* HARPISLGHMETCNAPSTATSTPRPAATSPEGR EEKVGCAPQNWQQLLHCHQTGHVLAPSPPTFV*Q KQGQIPATKTCC*LSLTGRI*DHCILS*SKVLE NLPHAQPETCSPSLPFGQTTGYNALPLLECC*P RPWTWLSTVRHTQTCRLF*NVLISLGNLEYRPLR P*PPKRAGILTHTGQPAVGSL*AYEPQSREVGT FSRGGSQL*TCQRTPRLATAQPSLTWMWLPRKR LNCEKVLRTHLTQACPKQRLEKRLLFSVFPKGAA LVVSKSLAPS*PLGSLCTLPPVSKMSLMSISCP GDS*GLCDLHKPTPGQAVGVSLVLRQILP*VPD LGPAAEGSL*AERSWPELYLFASC*PLTASRGIS FMSAYCIALSSCVPELLPFQ*DY*SCMSVSCLS SPFFLSRA*FYSGKTSPR*GPPPQQTSSRNCPI FTSWRDTLGFLYEAI**ACVLGTQQAGAWGLVDH HLVSVGGPGLPQVTST*GWRL*RQAHGQPCSLR LYCEAWGDVVFMLLPPSHWGFWMGNKKIKAVHF P

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT *PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD FHQQRSFLQRWPKSASLPRHEESHAGTGDLAVP VHARLARLPWASRPPGSTGASWQDRTKGRKGGAW PTRKEG*TWPPRCSWHAWAHRLARP*RTQG*KR *PGYDGLARVKRTNGLQGLPWIQRGKGIQR*KG *PGSQRRKGFPRISWNVGAER*NGSKR*TWDSRT PRTHRKTRKTRQAGTERG*WSYGPTRQAWAFWS TWPSGAPRPPTCRTTYNGTQRGKRISRASRKMS LWTHYECE*PFLRGICVWAQFPASSCDFCGQQPG GA*EAEHPKRHCLPQRPEISVLQGQPWLAPHPA DPFLPCGLHCRPARHLWGWAPAAWGGV*RR*QR CG*RLHPLSPCLLWRWSPA*GCGGL*RL*LWLPD MRDLSPWVIWRPAMHPVLLHRLHALPLLHLRAV RRRWAAPHRTGSSFSTVIKLAMSWPRLHQPLCD KNKDKFLLLRHAVNSV*LEEFRTTASCLNPRYWK TFHMPNLRPVPHRYHLAKQPATMLFLFWNVADP GRGLG*ALLDTHRPAGCFRMF*SLWGIWSTGLS APDHRNVQAFSLTLGSPLSGLSRPMNHKAGKWAR SLGVAHSFEPAKGPLDWPQPSPA*RGCGCPGKD LTVKKY*EPT*PRLAPSRG*RRGSSSQCFPKGR LLWFQNLWHHLDLLALSALCPLSQKCPSCPFPVQ ETHEDCVTCTSPHLGRLLVSPWSLGRYSPESPI *GQLQRALSRQSVPGQSSTSLPPADP*QRPVAF LSCLHIA*PCPPVCLSSSLFNKIISRACL*AAFH HHFS*VGLSFILERHLQGEVHPHSRPQVEIAQF LPAGGTPLGFCTKLFNEPVSWGLSRLELGAWWT ITWCL*VDPVSHR*HQPEGGVFRDRHMGSPVPFA STARPGEMLFSCCFHHHTGVSGWEIKK*RQFIS P

Sequence NM_080542

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC TTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGGCCACAAAGCATGCTGCCTGCTGACGCCTCCTCCA CCACCACTGTTCCCACCACCATTCTTCAGAGGTGGCCGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCC CGTGCATGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGGGAGCT TGGCCGACCAGGAAGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAA GGTGACCTGGGTATGATGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGG GTGACCTGGGTCCCAAAAGTTCAAGAACTCCTTGCACATTACCAAGACGGCCCCCAGTGCCATGTGGACAAGGCAGCCGGTCACCTGTCACCGTTGTGGC TGGAAAGGAGTCCCAAGCCTGCCTGCTCCCCAGATTTGAGGAAGATTACATTTCATCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAA GCCTGGGCCTTCTGGTCAACCTGGCCGTCCGGGGCCCCCAGGCCCCCCACCTGCAGGACAACTTATAATGGGACCCAAAGGGGAAAGAGGATTTCCCGGG CCTCCAGGAAGATGTCTTTGTGGACCCACTATGAATGTGAATAACCCTTCCTACGGGGAATCTGTGTATGGGCCCAGTTCCCCGCGAGTTCCTGTGATTT TTGTGGTCAACAACCAGGAGGAGCTTGAGAGGCTGAACACCCAAAACGCCATTGCCTTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGACAGCCTTGG CTGGCTCCCCATCCAGCTGACCCCTTTCTACCCTGTGGATTACACTGCAGACCAGCACGGCACCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGT GACGACGGTAACAGCGATGTGGGTGACGACTGCATCCGCTGTCACCGTGCCTACTGTGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCT CTGACTTTGGCTACCTGACATGCGAGACCTATCTCCCTGGGTCATATGGAGACCTGCAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTA CTTCACCTGAGGGCCGTGAGGAGAAGGTGGGCTGCGCCCCACAGAACTGGCAGCAGCTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCC ACCAACCTTTGTGTGACAAAAACAAGGACAAATTCCTGCTACTAAGACATGCTGTTAACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCT TAATCCAAGGTACTGGAAAACCTTCCACATGCCCAACCTGAGACCTGTTCCCCATCGCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTC TGGAATGTTGCTGACCCAGGCCGTGGACTTGGCTGAGCACTGTTAGACACACACAGACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCT GGAGTACAGGCCTCTCCGCCCCTGACCACCGAAACGTGCAGGCATTCTCACTCACACTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAA AGCAGGGAAGTGGGCACGTTCTCTCGGGGTGGCTCACAGCTTTGAACCTGCCAAAGGACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGT GGCTGCCCAGGAAAAGACTTAACTGTGAAAAAGTACTGAGAACCCACCTGACCCAGGCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGT GTTTCCCAAAGGGGCGGCTCTTGTGGTTTCAAAATCTCTGGCACCATCTTGACCTCTTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTC ATGTCCATTTCCTGTCCAGGAGACTCATGAGGACTGTGTGACCTGCACAAGCCCACACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATAC TCCCCTGAGTCCCCGATCTAGGGCCAGCTGCAGAGGGCTCTCTCTAGGCAGAGCGTTCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGAC AGCGTCCCGTGGCATTTCTTTCATGTCTGCATATTGCATAGCCTTGTCCTCCTGTGTGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATG TCTGTGAGCTGCCTTTCATCACCATTTTTCCTGAGTAGGGCTTAGTTTTATTCTGGAAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAA GTAGAAATTGCCCAATTTTTACCAGCTGGAGGGACACCCTTGGGTTTTTGTACGAAGCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGC TTGGGGCCTGGTGGACCATCACCTGGTGTCTGTAGGTGGACCCGGTCTCCCACAGGTGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGG CAGCCCTGTTCCCTTCGCCTCTACTGCGAGGCCTGGGGAGATGTTGTTTTCATGCTGCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAA TAAAGGCAGTTCATTTCCCCA

refseq_COLQ.F2 (forward primer)
refseq_COLQ.R2 (reverse primer)
Alternative acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF* LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT FINSVLPISAALPSLDQKKRGGHKACCLLTPPP PPLFPPPFFRGGRSPLLSPDMKNLMLELETSQS PCMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGEL GRPGRKGRPGPPGVPGMPGPIGWPGPEGPRGEK GDLGMMGLPGSRGPMGSKGYPGSRGEKGSRGEK GDLGPKSSRTPCTLPRRPPVPCGQGSRSPVTVVA GKESQACLLPRFEEDYISSGTERG*WSYGPTRQ AWAFWSTWPSGAPRPPTCRTTYNGTQRGKRISR ASRKMSLWTHYECE*PFLRGICVWAQFPASSCDF CGQQPGGA*EAEHPKRHCLPQRPEISVLQGQPW LAPHPADPFLPCGLHCRPARHLWGWAPAAWGGV *RR*QRCG*RLHPLSPCLLWRWSPA*GCGGL*RL *LWLPDMRDLSPWVIWRPAMHPVLLHRLHALPL LHLRAVRRRWAAPHRTGSSFSTVIKLAMSWPRL HQPLCDKNKDKFLLLRHAVNSV*LEEFRTTASCL NPRYWKTFHMPNLRPVPHRYHLAKQPATMLFLF WNVADPGRGLG*ALLDTHRPAGCFRMF*SLWGI WSTGLSAPDHRNVQAFSLTLGSPLSGLSRPMNHK AGKWARSLGVAHSFEPAKGPLDWPQPSPA*RGC GCPGKDLTVKKY*EPT*PRLAPSRG*RRGSSSQ CFPKGRLLWFQNLWHHLDLLALSALCPLSQKCPS CPFPVQETHEDCVTCTSPHLGRLLVSPWSLGRY SPESPI*GQLQRALSRQSVPGQSSTSLPPADP* QRPVAFLSCLHIA*PCPPVCLSSSLFNKIISRAC L*AAFHHHFS*VGLSFILERHLQGEVHPHSRPQ VEIAQFLPAGGTPLGFCTKLFNEPVSWGLSRLE LGAWWTITWCL*VDPVSHR*HQPEGGVFRDRHMG SPVPFASTARPGEMLFSCCFHHHTGVSGWEIKK *RQFISP

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR LSSTAFFQSQQPFPAWIRRSVVATKHAAC*RLLH HHCSHHHSSEVAEVRFSPQT*RISCWNWRPRSP RACKAR*APLGLPAPRVHRGFLARQDQREKRGS LADQEGRVDLAPQVFLACLGPSVGQALKDPGVKK VTWV*WACQGQEDQWAPRATLDPEGKRDPEVKR VTWVPKVQELLAHYQDGPQCHVDKAAGHLSPLW LERSPKPACSPDLRKITFHQGQKGDSGVMGPPGK PGPSGQPGRPGPPGPPPAGQLIMGPKGERGFPG PPGRCLCGPTMNVNNPSYGESVYGPSSPRVPVI FVVNNQEELERLNTQNAIAFRRDQRSLYFKDSLG WLPIQLTPFYPVDYTADQHGTCGDGLLQPGEEC DDGNSDVGDDCIRCHRAYCGDGHRHEGVEDCDG SDFGYLTCETYLPGSYGDLQCTQYCYIDSTPCRY FT*GP*GEGGLRPTELAAASPLSSNWPCPGPVS TNLCVTKTRTNSCY*DMLLTQSDWKNLGPLHPV LIQGTGKPSTCPT*DLFPIATIWPNNRLQCSSSS GMLLTQAVDLAEHC*THTDLQAVLECFDLFGES GVQASPPLTTETCRHSHSHWAARCRVSLGL*TT KQGSGHVLSGWLTALNLPKDPSTGHSPAQPDVDV AAQEKT*L*KSTENPPDPGLPQAEAREEAPLLS VSQRGGSCGFKISGTILTSWLSLHFAPCLKNVP HVHFLSRRLMRTV*PAQAHTWAGCWCLLGP*ADT PLSPRSRASCRGLSLGRAFLARALPLCLLLTPD SVPWHFFHVCILHSLVLLCA*APPFSIRLLVVH VCELPFITIFPE*GLVLFWKDISKVRSTPTADLK *KLPNFYQLEGHPWVFVRSYLMSLCLGDSAGWS LGPGGPSPGVCRWTRSPTGDINLRVASLETGTW AALFPSPLLRGLGRCCFHAASTITLGFLDGK*KN KGSSFP

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT *PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD FHQQRSSNLSSPSQPGSEEAWWPQSMLPADASS TTTVPTTILQRWPKSASLPRHEESHAGTGDLAVP VHARLARLPWASRPPGSTGASWQDRTKGRKGGA WPTRKEG*TWPPRCSWHAWAHRLARP*RTQG*K R*PGYDGLARVKRTNGLQGLPWIQRGKGIQR*KG *PGSQKFKNSLHITKTAPSAMWTRQPVTCHRCG WKGVPSLPAPQI*GRLHFIRDRKGIVELWAHQA SLGLLVNLAVRGPQAPHLQDNL*WDPKGKEDFPG LQEDVFVDPL*M*ITLPTGNLCMGPVPREFL*F LWSTTRRSLRG*TPKTPLPSAETRDLCTSRTAL AGSPSS*PLSTLWITLQTSTAPVGMGSCSLGRSV TTVTAMWVTTASAVTVPTVEMVTGMRVWRTVTA LTLAT*HARPISLGHMETCNAPSTATSTPRPAA TSPEGREEKVGCAPQNWQQLLHCHQTGHVLAPSP PTFV*QKQGQIPATKTCC*LSLTGRI*DHCILS *SKVLENLPHAQPETCSPSLPFGQTTGYNALPL LECC*PRPWTWLSTVRHTQTCRLF*NVLISLGNL EYRPLRP*PPKRAGILTHTGQPAVGSL*AYEPQ SREVGTFSRGGSQL*TCQRTPRLATAQPSLTWM WLPRKRLNCEKVLRTHLTQACPKQRLEKRLLFSV FPKGAALVVSKSLAPS*PLGSLCTLPPVSKMSL MSISCPGDS*GLCDLHKPTPGQAVGVSLVLRQI LP*VPDLGPAAEGSL*AERSWPELYLFASC*PLT ASRGISFMSAYCIALSSCVPELLPFQ*DY*SCM SVSCLSSPFFLSRA*FYSGKTSPR*GPPPQQTS SRNCPIFTSWRDTLGFLYEAI**ACVLGTQQAGA WGLVDHHLVSVGGPGLPQVTST*GWRL*RQAHG QPCSLRLYCEAWGDVVFMLLPPSHWGFWMGNKK IKAVHFP

Sequence NM_005677

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC TTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGGCCACAAAGCATGCTGCCTGCTGACGCCTCCTCCA CCACCACTGTTCCCACCACCATTCTTCAGAGGTGGCCGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCC CGTGCATGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGGGAGCT TGGCCGACCAGGAAGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAA GGTGACCTGGGTATGATGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGG GTGACCTGGGTCCCAAAGGAGAAAAGGGTTTCCCAGGATTTCCTGGAATGTTGGGGCAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGG ACACCGAGGACCCACAGGAAGACCAGGAAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTCTGGT CAACCTGGCCGTCCGGGGCCCCCAGGCCCCCCACCTGCAGGACAACTTATAATGGGACCCAAAGGGGAAAGAGGATTTCCCGGGCCTCCAGGAAGATGTC TTTGTGGACCCACTATGAATGTGAATAACCCTTCCTACGGGGAATCTGTGTATGGGCCCAGTTCCCCGCGAGTTCCTGTGATTTTTGTGGTCAACAACCA GGAGGAGCTTGAGAGGCTGAACACCCAAAACGCCATTGCCTTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGACAGCCTTGGCTGGCTCCCCATCCAG CTGACCCCTTTCTACCCTGTGGATTACACTGCAGACCAGCACGGCACCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGTGACGACGGTAACAGCG ATGTGGGTGACGACTGCATCCGCTGTCACCGTGCCTACTGTGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCTCTGACTTTGGCTACCT GACATGCGAGACCTATCTCCCTGGGTCATATGGAGACCTGCAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTACTTCACCTGAGGGCCG TGAGGAGAAGGTGGGCTGCGCCCCACAGAACTGGCAGCAGCTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCCACCAACCTTTGTGTGA CAAAAACAAGGACAAATTCCTGCTACTAAGACATGCTGTTAACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCTTAATCCAAGGTACTGG AAAACCTTCCACATGCCCAACCTGAGACCTGTTCCCCATCGCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTCTGGAATGTTGCTGACC CAGGCCGTGGACTTGGCTGAGCACTGTTAGACACACACAGACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCTGGAGTACAGGCCTCTC CGCCCCTGACCACCGAAACGTGCAGGCATTCTCACTCACACTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAAAGCAGGGAAGTGGGCA CGTTCTCTCGGGGTGGCTCACAGCTTTGAACCTGCCAAAGGACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGTGGCTGCCCAGGAAAAG ACTTAACTGTGAAAAAGTACTGAGAACCCACCTGACCCAGGCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGTGTTTCCCAAAGGGGCG GCTCTTGTGGTTTCAAAATCTCTGGCACCATCTTGACCTCTTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTCATGTCCATTTCCTGTC CAGGAGACTCATGAGGACTGTGTGACCTGCACAAGCCCACACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATACTCCCCTGAGTCCCCGA TCTAGGGCCAGCTGCAGAGGGCTCTCTCTAGGCAGAGCGTTCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGACAGCGTCCCGTGGCATT TCTTTCATGTCTGCATATTGCATAGCCTTGTCCTCCTGTGTGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATGTCTGTGAGCTGCCTTT CATCACCATTTTTCCTGAGTAGGGCTTAGTTTTATTCTGGAAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAAGTAGAAATTGCCCAAT TTTTACCAGCTGGAGGGACACCCTTGGGTTTTTGTACGAAGCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGCTTGGGGCCTGGTGGAC CATCACCTGGTGTCTGTAGGTGGACCCGGTCTCCCACAGGTGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGGCAGCCCTGTTCCCTTC GCCTCTACTGCGAGGCCTGGGGAGATGTTGTTTTCATGCTGCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAATAAAGGCAGTTCATTT CCCCA

refseq_COLQ.F2 (forward primer)
refseq_COLQ.R2 (reverse primer)
Alternative acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF* LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT FINSVLPISAALPSLDQKKRGGHKACCLLTPPP PPLFPPPFFRGGRSPLLSPDMKNLMLELETSQS PCMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGEL GRPGRKGRPGPPGVPGMPGPIGWPGPEGPRGEK GDLGMMGLPGSRGPMGSKGYPGSRGEKGSRGEK GDLGPKGEKGFPGFPGMLGQKGEMGPKGEPGIAG HRGPTGRPGKRGKQGQKGDSGVMGPPGKPGPSG QPGRPGPPGPPPAGQLIMGPKGERGFPGPPGRC LCGPTMNVNNPSYGESVYGPSSPRVPVIFVVNNQ EELERLNTQNAIAFRRDQRSLYFKDSLGWLPIQ LTPFYPVDYTADQHGTCGDGLLQPGEECDDGNS DVGDDCIRCHRAYCGDGHRHEGVEDCDGSDFGYL TCETYLPGSYGDLQCTQYCYIDSTPCRYFT*GP *GEGGLRPTELAAASPLSSNWPCPGPVSTNLCV TKTRTNSCY*DMLLTQSDWKNLGPLHPVLIQGTG KPSTCPT*DLFPIATIWPNNRLQCSSSSGMLLT QAVDLAEHC*THTDLQAVLECFDLFGESGVQAS PPLTTETCRHSHSHWAARCRVSLGL*TTKQGSGH VLSGWLTALNLPKDPSTGHSPAQPDVDVAAQEK T*L*KSTENPPDPGLPQAEAREEAPLLSVSQRG GSCGFKISGTILTSWLSLHFAPCLKNVPHVHFLS RRLMRTV*PAQAHTWAGCWCLLGP*ADTPLSPR SRASCRGLSLGRAFLARALPLCLLLTPDSVPWH FFHVCILHSLVLLCA*APPFSIRLLVVHVCELPF ITIFPE*GLVLFWKDISKVRSTPTADLK*KLPN FYQLEGHPWVFVRSYLMSLCLGDSAGWSLGPGG PSPGVCRWTRSPTGDINLRVASLETGTWAALFPS PLLRGLGRCCFHAASTITLGFLDGK*KNKGSSF P

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR LSSTAFFQSQQPFPAWIRRSVVATKHAAC*RLLH HHCSHHHSSEVAEVRFSPQT*RISCWNWRPRSP RACKAR*APLGLPAPRVHRGFLARQDQREKRGS LADQEGRVDLAPQVFLACLGPSVGQALKDPGVKK VTWV*WACQGQEDQWAPRATLDPEGKRDPEVKR VTWVPKEKRVSQDFLECWGRKVKWVQKVNLG*Q DTEDPQEDQENEASRDRKGIVELWAHQASLGLLV NLAVRGPQAPHLQDNL*WDPKGKEDFPGLQEDV FVDPL*M*ITLPTGNLCMGPVPREFL*FLWSTT RRSLRG*TPKTPLPSAETRDLCTSRTALAGSPSS *PLSTLWITLQTSTAPVGMGSCSLGRSVTTVTA MWVTTASAVTVPTVEMVTGMRVWRTVTALTLAT *HARPISLGHMETCNAPSTATSTPRPAATSPEGR EEKVGCAPQNWQQLLHCHQTGHVLAPSPPTFV* QKQGQIPATKTCC*LSLTGRI*DHCILS*SKVL ENLPHAQPETCSPSLPFGQTTGYNALPLLECC*P RPWTWLSTVRHTQTCRLF*NVLISLGNLEYRPL RP*PPKRAGILTHTGQPAVGSL*AYEPQSREVG TFSRGGSQL*TCQRTPRLATAQPSLTWMWLPRKR LNCEKVLRTHLTQACPKQRLEKRLLFSVFPKGA ALVVSKSLAPS*PLGSLCTLPPVSKMSLMSISC PGDS*GLCDLHKPTPGQAVGVSLVLRQILP*VPD LGPAAEGSL*AERSWPELYLFASC*PLTASRGI SFMSAYCIALSSCVPELLPFQ*DY*SCMSVSCL SSPFFLSRA*FYSGKTSPR*GPPPQQTSSRNCPI FTSWRDTLGFLYEAI**ACVLGTQQAGAWGLVD HHLVSVGGPGLPQVTST*GWRL*RQAHGQPCSL RLYCEAWGDVVFMLLPPSHWGFWMGNKKIKAVHF P

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT *PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD FHQQRSSNLSSPSQPGSEEAWWPQSMLPADASS TTTVPTTILQRWPKSASLPRHEESHAGTGDLAVP VHARLARLPWASRPPGSTGASWQDRTKGRKGGA WPTRKEG*TWPPRCSWHAWAHRLARP*RTQG*K R*PGYDGLARVKRTNGLQGLPWIQRGKGIQR*KG *PGSQRRKGFPRISWNVGAER*NGSKR*TWDSR TPRTHRKTRKTRQAGTERG*WSYGPTRQAWAFW STWPSGAPRPPTCRTTYNGTQRGKRISRASRKMS LWTHYECE*PFLRGICVWAQFPASSCDFCGQQP GGA*EAEHPKRHCLPQRPEISVLQGQPWLAPHP ADPFLPCGLHCRPARHLWGWAPAAWGGV*RR*QR CG*RLHPLSPCLLWRWSPA*GCGGL*RL*LWLP DMRDLSPWVIWRPAMHPVLLHRLHALPLLHLRA VRRRWAAPHRTGSSFSTVIKLAMSWPRLHQPLCD KNKDKFLLLRHAVNSV*LEEFRTTASCLNPRYW KTFHMPNLRPVPHRYHLAKQPATMLFLFWNVAD PGRGLG*ALLDTHRPAGCFRMF*SLWGIWSTGLS APDHRNVQAFSLTLGSPLSGLSRPMNHKAGKWA RSLGVAHSFEPAKGPLDWPQPSPA*RGCGCPGK DLTVKKY*EPT*PRLAPSRG*RRGSSSQCFPKGR LLWFQNLWHHLDLLALSALCPLSQKCPSCPFPV QETHEDCVTCTSPHLGRLLVSPWSLGRYSPESP I*GQLQRALSRQSVPGQSSTSLPPADP*QRPVAF LSCLHIA*PCPPVCLSSSLFNKIISRACL*AAF HHHFS*VGLSFILERHLQGEVHPHSRPQVEIAQ FLPAGGTPLGFCTKLFNEPVSWGLSRLELGAWWT ITWCL*VDPVSHR*HQPEGGVFRDRHMGSPVPF ASTARPGEMLFSCCFHHHTGVSGWEIKK*RQFI SP

Sequence NM_080543

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC TTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGGCCACAAAGCATGCTGCCTGCTGACGCCTCCTCCA CCACCACTGTTCCCACCACCATTCTTCAGAGGTGGCCGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCC CGTGCATGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGGGAGCT TGGCCGACCAGGAAGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAA GGTGACCTGGGTATGATGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGG GTGACCTGGGTCCCAAAGGAGAAAAGGGTTTCCCAGGATTTCCTGGAATGTTGGGGCAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGG ACACCGAGGACCCACAGGAAGACCAGGAAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTCTGGT CAACCTGGCCGTCCGGGGCCCCCAGGCCCCCCACCTGCAGATTTTTGTGGTCAACAACCAGGAGGAGCTTGAGAGGCTGAACACCCAAAACGCCATTGCC TTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGACAGCCTTGGCTGGCTCCCCATCCAGCTGACCCCTTTCTACCCTGTGGATTACACTGCAGACCAGC ACGGCACCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGTGACGACGGTAACAGCGATGTGGGTGACGACTGCATCCGCTGTCACCGTGCCTACTG TGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCTCTGACTTTGGCTACCTGACATGCGAGACCTATCTCCCTGGGTCATATGGAGACCTG CAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTACTTCACCTGAGGGCCGTGAGGAGAAGGTGGGCTGCGCCCCACAGAACTGGCAGCAG CTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCCACCAACCTTTGTGTGACAAAAACAAGGACAAATTCCTGCTACTAAGACATGCTGTT AACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCTTAATCCAAGGTACTGGAAAACCTTCCACATGCCCAACCTGAGACCTGTTCCCCATC GCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTCTGGAATGTTGCTGACCCAGGCCGTGGACTTGGCTGAGCACTGTTAGACACACACAG ACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCTGGAGTACAGGCCTCTCCGCCCCTGACCACCGAAACGTGCAGGCATTCTCACTCACA CTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAAAGCAGGGAAGTGGGCACGTTCTCTCGGGGTGGCTCACAGCTTTGAACCTGCCAAAG GACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGTGGCTGCCCAGGAAAAGACTTAACTGTGAAAAAGTACTGAGAACCCACCTGACCCAG GCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGTGTTTCCCAAAGGGGCGGCTCTTGTGGTTTCAAAATCTCTGGCACCATCTTGACCTC TTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTCATGTCCATTTCCTGTCCAGGAGACTCATGAGGACTGTGTGACCTGCACAAGCCCAC ACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATACTCCCCTGAGTCCCCGATCTAGGGCCAGCTGCAGAGGGCTCTCTCTAGGCAGAGCGT TCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGACAGCGTCCCGTGGCATTTCTTTCATGTCTGCATATTGCATAGCCTTGTCCTCCTGTG TGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATGTCTGTGAGCTGCCTTTCATCACCATTTTTCCTGAGTAGGGCTTAGTTTTATTCTGG AAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAAGTAGAAATTGCCCAATTTTTACCAGCTGGAGGGACACCCTTGGGTTTTTGTACGAA GCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGCTTGGGGCCTGGTGGACCATCACCTGGTGTCTGTAGGTGGACCCGGTCTCCCACAGG TGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGGCAGCCCTGTTCCCTTCGCCTCTACTGCGAGGCCTGGGGAGATGTTGTTTTCATGCT GCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAATAAAGGCAGTTCATTTCCCCA

refseq_COLQ.F2 (forward primer)
refseq_COLQ.R2 (reverse primer)
Alternative acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF* LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT FINSVLPISAALPSLDQKKRGGHKACCLLTPPP PPLFPPPFFRGGRSPLLSPDMKNLMLELETSQS PCMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGEL GRPGRKGRPGPPGVPGMPGPIGWPGPEGPRGEK GDLGMMGLPGSRGPMGSKGYPGSRGEKGSRGEK GDLGPKGEKGFPGFPGMLGQKGEMGPKGEPGIAG HRGPTGRPGKRGKQGQKGDSGVMGPPGKPGPSG QPGRPGPPGPPPADFCGQQPGGA*EAEHPKRHC LPQRPEISVLQGQPWLAPHPADPFLPCGLHCRPA RHLWGWAPAAWGGV*RR*QRCG*RLHPLSPCLL WRWSPA*GCGGL*RL*LWLPDMRDLSPWVIWRP AMHPVLLHRLHALPLLHLRAVRRRWAAPHRTGSS FSTVIKLAMSWPRLHQPLCDKNKDKFLLLRHAV NSV*LEEFRTTASCLNPRYWKTFHMPNLRPVPH RYHLAKQPATMLFLFWNVADPGRGLG*ALLDTHR PAGCFRMF*SLWGIWSTGLSAPDHRNVQAFSLT LGSPLSGLSRPMNHKAGKWARSLGVAHSFEPAK GPLDWPQPSPA*RGCGCPGKDLTVKKY*EPT*PR LAPSRG*RRGSSSQCFPKGRLLWFQNLWHHLDL LALSALCPLSQKCPSCPFPVQETHEDCVTCTSP HLGRLLVSPWSLGRYSPESPI*GQLQRALSRQSV PGQSSTSLPPADP*QRPVAFLSCLHIA*PCPPV CLSSSLFNKIISRACL*AAFHHHFS*VGLSFIL ERHLQGEVHPHSRPQVEIAQFLPAGGTPLGFCTK LFNEPVSWGLSRLELGAWWTITWCL*VDPVSHR *HQPEGGVFRDRHMGSPVPFASTARPGEMLFSC CFHHHTGVSGWEIKK*RQFISP

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR LSSTAFFQSQQPFPAWIRRSVVATKHAAC*RLLH HHCSHHHSSEVAEVRFSPQT*RISCWNWRPRSP RACKAR*APLGLPAPRVHRGFLARQDQREKRGS LADQEGRVDLAPQVFLACLGPSVGQALKDPGVKK VTWV*WACQGQEDQWAPRATLDPEGKRDPEVKR VTWVPKEKRVSQDFLECWGRKVKWVQKVNLG*Q DTEDPQEDQENEASRDRKGIVELWAHQASLGLLV NLAVRGPQAPHLQIFVVNNQEELERLNTQNAIA FRRDQRSLYFKDSLGWLPIQLTPFYPVDYTADQ HGTCGDGLLQPGEECDDGNSDVGDDCIRCHRAYC GDGHRHEGVEDCDGSDFGYLTCETYLPGSYGDL QCTQYCYIDSTPCRYFT*GP*GEGGLRPTELAA ASPLSSNWPCPGPVSTNLCVTKTRTNSCY*DMLL TQSDWKNLGPLHPVLIQGTGKPSTCPT*DLFPI ATIWPNNRLQCSSSSGMLLTQAVDLAEHC*THT DLQAVLECFDLFGESGVQASPPLTTETCRHSHSH WAARCRVSLGL*TTKQGSGHVLSGWLTALNLPK DPSTGHSPAQPDVDVAAQEKT*L*KSTENPPDP GLPQAEAREEAPLLSVSQRGGSCGFKISGTILTS WLSLHFAPCLKNVPHVHFLSRRLMRTV*PAQAH TWAGCWCLLGP*ADTPLSPRSRASCRGLSLGRA FLARALPLCLLLTPDSVPWHFFHVCILHSLVLLC A*APPFSIRLLVVHVCELPFITIFPE*GLVLFW KDISKVRSTPTADLK*KLPNFYQLEGHPWVFVR SYLMSLCLGDSAGWSLGPGGPSPGVCRWTRSPTG DINLRVASLETGTWAALFPSPLLRGLGRCCFHA ASTITLGFLDGK*KNKGSSFP

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT *PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD FHQQRSSNLSSPSQPGSEEAWWPQSMLPADASS TTTVPTTILQRWPKSASLPRHEESHAGTGDLAVP VHARLARLPWASRPPGSTGASWQDRTKGRKGGA WPTRKEG*TWPPRCSWHAWAHRLARP*RTQG*K R*PGYDGLARVKRTNGLQGLPWIQRGKGIQR*KG *PGSQRRKGFPRISWNVGAER*NGSKR*TWDSR TPRTHRKTRKTRQAGTERG*WSYGPTRQAWAFW STWPSGAPRPPTCRFLWSTTRRSLRG*TPKTPLP SAETRDLCTSRTALAGSPSS*PLSTLWITLQTS TAPVGMGSCSLGRSVTTVTAMWVTTASAVTVPT VEMVTGMRVWRTVTALTLAT*HARPISLGHMETC NAPSTATSTPRPAATSPEGREEKVGCAPQNWQQ LLHCHQTGHVLAPSPPTFV*QKQGQIPATKTCC *LSLTGRI*DHCILS*SKVLENLPHAQPETCSPS LPFGQTTGYNALPLLECC*PRPWTWLSTVRHTQ TCRLF*NVLISLGNLEYRPLRP*PPKRAGILTH TGQPAVGSL*AYEPQSREVGTFSRGGSQL*TCQR TPRLATAQPSLTWMWLPRKRLNCEKVLRTHLTQ ACPKQRLEKRLLFSVFPKGAALVVSKSLAPS*P LGSLCTLPPVSKMSLMSISCPGDS*GLCDLHKPT PGQAVGVSLVLRQILP*VPDLGPAAEGSL*AER SWPELYLFASC*PLTASRGISFMSAYCIALSSC VPELLPFQ*DY*SCMSVSCLSSPFFLSRA*FYSG KTSPR*GPPPQQTSSRNCPIFTSWRDTLGFLYE AI**ACVLGTQQAGAWGLVDHHLVSVGGPGLPQ VTST*GWRL*RQAHGQPCSLRLYCEAWGDVVFML LPPSHWGFWMGNKKIKAVHFP

Sequence NM_080544

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC TTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGGCCACAAAGCATGCTGCCTGCTGACGCCTCCTCCA CCACCACTGTTCCCACCACCATTCTTCAGAGGTGGCCGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCC CGTGCATGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGGGAGCT TGGCCGACCAGGAAGGAAGGGTAGACCTGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAA GGTGACCTGGGTATGATGGGCTTGCCAGGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGG GTGACCTGGGTCCCAAAGGAGAAAAGGGTTTCCCAGGATTTCCTGGAATGTTGGGGCAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGG ACACCGAGGACCCACAGGAAGACCAGGAAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTCTGGT CAACCTGGCCGTCCGGGGCCCCCAGGCCCCCCACCTGCAGGTCATATGGAGACCTGCAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTA CTTCACCTGAGGGCCGTGAGGAGAAGGTGGGCTGCGCCCCACAGAACTGGCAGCAGCTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCC ACCAACCTTTGTGTGACAAAAACAAGGACAAATTCCTGCTACTAAGACATGCTGTTAACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCT TAATCCAAGGTACTGGAAAACCTTCCACATGCCCAACCTGAGACCTGTTCCCCATCGCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTC TGGAATGTTGCTGACCCAGGCCGTGGACTTGGCTGAGCACTGTTAGACACACACAGACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCT GGAGTACAGGCCTCTCCGCCCCTGACCACCGAAACGTGCAGGCATTCTCACTCACACTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAA AGCAGGGAAGTGGGCACGTTCTCTCGGGGTGGCTCACAGCTTTGAACCTGCCAAAGGACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGT GGCTGCCCAGGAAAAGACTTAACTGTGAAAAAGTACTGAGAACCCACCTGACCCAGGCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGT GTTTCCCAAAGGGGCGGCTCTTGTGGTTTCAAAATCTCTGGCACCATCTTGACCTCTTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTC ATGTCCATTTCCTGTCCAGGAGACTCATGAGGACTGTGTGACCTGCACAAGCCCACACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATAC TCCCCTGAGTCCCCGATCTAGGGCCAGCTGCAGAGGGCTCTCTCTAGGCAGAGCGTTCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGAC AGCGTCCCGTGGCATTTCTTTCATGTCTGCATATTGCATAGCCTTGTCCTCCTGTGTGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATG TCTGTGAGCTGCCTTTCATCACCATTTTTCCTGAGTAGGGCTTAGTTTTATTCTGGAAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAA GTAGAAATTGCCCAATTTTTACCAGCTGGAGGGACACCCTTGGGTTTTTGTACGAAGCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGC TTGGGGCCTGGTGGACCATCACCTGGTGTCTGTAGGTGGACCCGGTCTCCCACAGGTGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGG CAGCCCTGTTCCCTTCGCCTCTACTGCGAGGCCTGGGGAGATGTTGTTTTCATGCTGCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAA TAAAGGCAGTTCATTTCCCCA

refseq_COLQ.F2 (forward primer)
refseq_COLQ.R2 (reverse primer)
Alternative acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF* LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT FINSVLPISAALPSLDQKKRGGHKACCLLTPPP PPLFPPPFFRGGRSPLLSPDMKNLMLELETSQS PCMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGEL GRPGRKGRPGPPGVPGMPGPIGWPGPEGPRGEK GDLGMMGLPGSRGPMGSKGYPGSRGEKGSRGEK GDLGPKGEKGFPGFPGMLGQKGEMGPKGEPGIAG HRGPTGRPGKRGKQGQKGDSGVMGPPGKPGPSG QPGRPGPPGPPPAGHMETCNAPSTATSTPRPAA TSPEGREEKVGCAPQNWQQLLHCHQTGHVLAPSP PTFV*QKQGQIPATKTCC*LSLTGRI*DHCILS *SKVLENLPHAQPETCSPSLPFGQTTGYNALPL LECC*PRPWTWLSTVRHTQTCRLF*NVLISLGNL EYRPLRP*PPKRAGILTHTGQPAVGSL*AYEPQ SREVGTFSRGGSQL*TCQRTPRLATAQPSLTWM WLPRKRLNCEKVLRTHLTQACPKQRLEKRLLFSV FPKGAALVVSKSLAPS*PLGSLCTLPPVSKMSL MSISCPGDS*GLCDLHKPTPGQAVGVSLVLRQI LP*VPDLGPAAEGSL*AERSWPELYLFASC*PLT ASRGISFMSAYCIALSSCVPELLPFQ*DY*SCM SVSCLSSPFFLSRA*FYSGKTSPR*GPPPQQTS SRNCPIFTSWRDTLGFLYEAI**ACVLGTQQAGA WGLVDHHLVSVGGPGLPQVTST*GWRL*RQAHG QPCSLRLYCEAWGDVVFMLLPPSHWGFWMGNKK IKAVHFP

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR LSSTAFFQSQQPFPAWIRRSVVATKHAAC*RLLH HHCSHHHSSEVAEVRFSPQT*RISCWNWRPRSP RACKAR*APLGLPAPRVHRGFLARQDQREKRGS LADQEGRVDLAPQVFLACLGPSVGQALKDPGVKK VTWV*WACQGQEDQWAPRATLDPEGKRDPEVKR VTWVPKEKRVSQDFLECWGRKVKWVQKVNLG*Q DTEDPQEDQENEASRDRKGIVELWAHQASLGLLV NLAVRGPQAPHLQVIWRPAMHPVLLHRLHALPL LHLRAVRRRWAAPHRTGSSFSTVIKLAMSWPRL HQPLCDKNKDKFLLLRHAVNSV*LEEFRTTASCL NPRYWKTFHMPNLRPVPHRYHLAKQPATMLFLF WNVADPGRGLG*ALLDTHRPAGCFRMF*SLWGI WSTGLSAPDHRNVQAFSLTLGSPLSGLSRPMNHK AGKWARSLGVAHSFEPAKGPLDWPQPSPA*RGC GCPGKDLTVKKY*EPT*PRLAPSRG*RRGSSSQ CFPKGRLLWFQNLWHHLDLLALSALCPLSQKCPS CPFPVQETHEDCVTCTSPHLGRLLVSPWSLGRY SPESPI*GQLQRALSRQSVPGQSSTSLPPADP* QRPVAFLSCLHIA*PCPPVCLSSSLFNKIISRAC L*AAFHHHFS*VGLSFILERHLQGEVHPHSRPQ VEIAQFLPAGGTPLGFCTKLFNEPVSWGLSRLE LGAWWTITWCL*VDPVSHR*HQPEGGVFRDRHMG SPVPFASTARPGEMLFSCCFHHHTGVSGWEIKK *RQFISP

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT *PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD FHQQRSSNLSSPSQPGSEEAWWPQSMLPADASS TTTVPTTILQRWPKSASLPRHEESHAGTGDLAVP VHARLARLPWASRPPGSTGASWQDRTKGRKGGA WPTRKEG*TWPPRCSWHAWAHRLARP*RTQG*K R*PGYDGLARVKRTNGLQGLPWIQRGKGIQR*KG *PGSQRRKGFPRISWNVGAER*NGSKR*TWDSR TPRTHRKTRKTRQAGTERG*WSYGPTRQAWAFW STWPSGAPRPPTCRSYGDLQCTQYCYIDSTPCRY FT*GP*GEGGLRPTELAAASPLSSNWPCPGPVS TNLCVTKTRTNSCY*DMLLTQSDWKNLGPLHPV LIQGTGKPSTCPT*DLFPIATIWPNNRLQCSSSS GMLLTQAVDLAEHC*THTDLQAVLECFDLFGES GVQASPPLTTETCRHSHSHWAARCRVSLGL*TT KQGSGHVLSGWLTALNLPKDPSTGHSPAQPDVDV AAQEKT*L*KSTENPPDPGLPQAEAREEAPLLS VSQRGGSCGFKISGTILTSWLSLHFAPCLKNVP HVHFLSRRLMRTV*PAQAHTWAGCWCLLGP*ADT PLSPRSRASCRGLSLGRAFLARALPLCLLLTPD SVPWHFFHVCILHSLVLLCA*APPFSIRLLVVH VCELPFITIFPE*GLVLFWKDISKVRSTPTADLK *KLPNFYQLEGHPWVFVRSYLMSLCLGDSAGWS LGPGGPSPGVCRWTRSPTGDINLRVASLETGTW AALFPSPLLRGLGRCCFHAASTITLGFLDGK*KN KGSSFP

Sequence NM_080541

CACACGCACACACACACACACAAACACACGCGCACGCAAACAGCAGTGAGGAAGAGAAAAGCAGAGATAAGCAAGGCTCCGCAGCAGCCTCCTTTCTAAC TTGACCCTCGCCAGACCCTGGCCAGCATGGTTGTCCTGAATCCAATGACTTTGGGAATTTATCTTCAGCTTTTCTTCCTCTCTATCGTGTCTCAGCCGAC TTTCATCAACAGCGTTCTTCCAATCTCAGCAGCCCTTCCCAGCCTGGATCAGAAGAAGCGTGGTGGCCACAAAGCATGCTGCCTGCTGACGCCTCCTCCA CCACCACTGTTCCCACCACCATTCTTCAGAGGTGGCCGAAGTCCGCTTCTCTCCCCAGACATGAAGAATCTCATGCTGGAACTGGAGACCTCGCAGTCCC CGTGCATGCAAGGCTCGCTAGGCTCCCCTGGGCCTCCCGGCCCCCAGGGTCCACCGGGGCTTCCTGGCAAGACAGGACCAAAGGGAGAAAAGGGTAGACC TGGCCCCCCAGGTGTTCCTGGCATGCCTGGGCCCATCGGTTGGCCAGGCCCTGAAGGACCCAGGGGTGAAAAAGGTGACCTGGGTATGATGGGCTTGCCA GGGTCAAGAGGACCAATGGGCTCCAAGGGCTACCCTGGATCCAGAGGGGAAAAGGGATCCAGAGGTGAAAAGGGTGACCTGGGTCCCAAAGGAGAAAAGG GTTTCCCAGGATTTCCTGGAATGTTGGGGCAGAAAGGTGAAATGGGTCCAAAAGGTGAACCTGGGATAGCAGGACACCGAGGACCCACAGGAAGACCAGG AAAACGAGGCAAGCAGGGACAGAAAGGGGATAGTGGAGTTATGGGCCCACCAGGCAAGCCTGGGCCTTCTGGTCAACCTGGCCGTCCGGGGCCCCCAGGC CCCCCACCTGCAGGACAACTTATAATGGGACCCAAAGGGGAAAGAGGATTTCCCGGGCCTCCAGGAAGATGTCTTTGTGGACCCACTATGAATGTGAATA ACCCTTCCTACGGGGAATCTGTGTATGGGCCCAGTTCCCCGCGAGTTCCTGTGATTTTTGTGGTCAACAACCAGGAGGAGCTTGAGAGGCTGAACACCCA AAACGCCATTGCCTTCCGCAGAGACCAGAGATCTCTGTACTTCAAGGACAGCCTTGGCTGGCTCCCCATCCAGCTGACCCCTTTCTACCCTGTGGATTAC ACTGCAGACCAGCACGGCACCTGTGGGGATGGGCTCCTGCAGCCTGGGGAGGAGTGTGACGACGGTAACAGCGATGTGGGTGACGACTGCATCCGCTGTC ACCGTGCCTACTGTGGAGATGGTCACCGGCATGAGGGTGTGGAGGACTGTGACGGCTCTGACTTTGGCTACCTGACATGCGAGACCTATCTCCCTGGGTC ATATGGAGACCTGCAATGCACCCAGTACTGCTACATCGACTCCACGCCCTGCCGCTACTTCACCTGAGGGCCGTGAGGAGAAGGTGGGCTGCGCCCCACA GAACTGGCAGCAGCTTCTCCACTGTCATCAAACTGGCCATGTCCTGGCCCCGTCTCCACCAACCTTTGTGTGACAAAAACAAGGACAAATTCCTGCTACT AAGACATGCTGTTAACTCAGTCTGACTGGAAGAATTTAGGACCACTGCATCCTGTCTTAATCCAAGGTACTGGAAAACCTTCCACATGCCCAACCTGAGA CCTGTTCCCCATCGCTACCATTTGGCCAAACAACCGGCTACAATGCTCTTCCTCTTCTGGAATGTTGCTGACCCAGGCCGTGGACTTGGCTGAGCACTGT TAGACACACACAGACCTGCAGGCTGTTTTAGAATGTTTTGATCTCTTTGGGGAATCTGGAGTACAGGCCTCTCCGCCCCTGACCACCGAAACGTGCAGGC ATTCTCACTCACACTGGGCAGCCCGCTGTCGGGTCTCTCTAGGCCTATGAACCACAAAGCAGGGAAGTGGGCACGTTCTCTCGGGGTGGCTCACAGCTTT GAACCTGCCAAAGGACCCCTCGACTGGCCACAGCCCAGCCCAGCCTGACGTGGATGTGGCTGCCCAGGAAAAGACTTAACTGTGAAAAAGTACTGAGAAC CCACCTGACCCAGGCTTGCCCCAAGCAGAGGCTAGAGAAGAGGCTCCTCTTCTCAGTGTTTCCCAAAGGGGCGGCTCTTGTGGTTTCAAAATCTCTGGCA CCATCTTGACCTCTTGGCTCTCTCTGCACTTTGCCCCCTGTCTCAAAAATGTCCCTCATGTCCATTTCCTGTCCAGGAGACTCATGAGGACTGTGTGACC TGCACAAGCCCACACCTGGGCAGGCTGTTGGTGTCTCCTTGGTCCTTAGGCAGATACTCCCCTGAGTCCCCGATCTAGGGCCAGCTGCAGAGGGCTCTCT CTAGGCAGAGCGTTCCTGGCCAGAGCTCTACCTCTTTGCCTCCTGCTGACCCCTGACAGCGTCCCGTGGCATTTCTTTCATGTCTGCATATTGCATAGCC TTGTCCTCCTGTGTGCCTGAGCTCCTCCCTTTTCAATAAGATTATTAGTCGTGCATGTCTGTGAGCTGCCTTTCATCACCATTTTTCCTGAGTAGGGCTT AGTTTTATTCTGGAAAGACATCTCCAAGGTGAGGTCCACCCCCACAGCAGACCTCAAGTAGAAATTGCCCAATTTTTACCAGCTGGAGGGACACCCTTGG GTTTTTGTACGAAGCTATTTAATGAGCCTGTGTCTTGGGGACTCAGCAGGCTGGAGCTTGGGGCCTGGTGGACCATCACCTGGTGTCTGTAGGTGGACCC GGTCTCCCACAGGTGACATCAACCTGAGGGTGGCGTCTTTAGAGACAGGCACATGGGCAGCCCTGTTCCCTTCGCCTCTACTGCGAGGCCTGGGGAGATG TTGTTTTCATGCTGCTTCCACCATCACACTGGGGTTTCTGGATGGGAAATAAAAAAATAAAGGCAGTTCATTTCCCCA

refseq_COLQ.F2 (forward primer)
refseq_COLQ.R2 (reverse primer)
Alternative acceptor

Frame 0

HTHTHTHKHTRTQTAVRKRKAEISKAPQQPPF* LDPRQTLASMVVLNPMTLGIYLQLFFLSIVSQPT FINSVLPISAALPSLDQKKRGGHKACCLLTPPP PPLFPPPFFRGGRSPLLSPDMKNLMLELETSQS PCMQGSLGSPGPPGPQGPPGLPGKTGPKGEKGRP GPPGVPGMPGPIGWPGPEGPRGEKGDLGMMGLP GSRGPMGSKGYPGSRGEKGSRGEKGDLGPKGEK GFPGFPGMLGQKGEMGPKGEPGIAGHRGPTGRPG KRGKQGQKGDSGVMGPPGKPGPSGQPGRPGPPG PPPAGQLIMGPKGERGFPGPPGRCLCGPTMNVN NPSYGESVYGPSSPRVPVIFVVNNQEELERLNTQ NAIAFRRDQRSLYFKDSLGWLPIQLTPFYPVDY TADQHGTCGDGLLQPGEECDDGNSDVGDDCIRC HRAYCGDGHRHEGVEDCDGSDFGYLTCETYLPGS YGDLQCTQYCYIDSTPCRYFT*GP*GEGGLRPT ELAAASPLSSNWPCPGPVSTNLCVTKTRTNSCY *DMLLTQSDWKNLGPLHPVLIQGTGKPSTCPT*D LFPIATIWPNNRLQCSSSSGMLLTQAVDLAEHC *THTDLQAVLECFDLFGESGVQASPPLTTETCR HSHSHWAARCRVSLGL*TTKQGSGHVLSGWLTAL NLPKDPSTGHSPAQPDVDVAAQEKT*L*KSTEN PPDPGLPQAEAREEAPLLSVSQRGGSCGFKISG TILTSWLSLHFAPCLKNVPHVHFLSRRLMRTV*P AQAHTWAGCWCLLGP*ADTPLSPRSRASCRGLS LGRAFLARALPLCLLLTPDSVPWHFFHVCILHS LVLLCA*APPFSIRLLVVHVCELPFITIFPE*GL VLFWKDISKVRSTPTADLK*KLPNFYQLEGHPW VFVRSYLMSLCLGDSAGWSLGPGGPSPGVCRWT RSPTGDINLRVASLETGTWAALFPSPLLRGLGRC CFHAASTITLGFLDGK*KNKGSSFP

Frame 1

TRTHTHTNTRARKQQ*GREKQR*ARLRSSLLSN LTLARPWPAWLS*IQ*LWEFIFSFSSSLSCLSR LSSTAFFQSQQPFPAWIRRSVVATKHAAC*RLLH HHCSHHHSSEVAEVRFSPQT*RISCWNWRPRSP RACKAR*APLGLPAPRVHRGFLARQDQREKRVD LAPQVFLACLGPSVGQALKDPGVKKVTWV*WACQ GQEDQWAPRATLDPEGKRDPEVKRVTWVPKEKR VSQDFLECWGRKVKWVQKVNLG*QDTEDPQEDQ ENEASRDRKGIVELWAHQASLGLLVNLAVRGPQA PHLQDNL*WDPKGKEDFPGLQEDVFVDPL*M*I TLPTGNLCMGPVPREFL*FLWSTTRRSLRG*TP KTPLPSAETRDLCTSRTALAGSPSS*PLSTLWIT LQTSTAPVGMGSCSLGRSVTTVTAMWVTTASAV TVPTVEMVTGMRVWRTVTALTLAT*HARPISLG HMETCNAPSTATSTPRPAATSPEGREEKVGCAPQ NWQQLLHCHQTGHVLAPSPPTFV*QKQGQIPAT KTCC*LSLTGRI*DHCILS*SKVLENLPHAQPE TCSPSLPFGQTTGYNALPLLECC*PRPWTWLSTV RHTQTCRLF*NVLISLGNLEYRPLRP*PPKRAG ILTHTGQPAVGSL*AYEPQSREVGTFSRGGSQL *TCQRTPRLATAQPSLTWMWLPRKRLNCEKVLRT HLTQACPKQRLEKRLLFSVFPKGAALVVSKSLA PS*PLGSLCTLPPVSKMSLMSISCPGDS*GLCD LHKPTPGQAVGVSLVLRQILP*VPDLGPAAEGSL *AERSWPELYLFASC*PLTASRGISFMSAYCIA LSSCVPELLPFQ*DY*SCMSVSCLSSPFFLSRA *FYSGKTSPR*GPPPQQTSSRNCPIFTSWRDTLG FLYEAI**ACVLGTQQAGAWGLVDHHLVSVGGP GLPQVTST*GWRL*RQAHGQPCSLRLYCEAWGD VVFMLLPPSHWGFWMGNKKIKAVHFP

Frame 2

HAHTHTQTHAHANSSEEEKSRDKQGSAAASFLT *PSPDPGQHGCPESNDFGNLSSAFLPLYRVSAD FHQQRSSNLSSPSQPGSEEAWWPQSMLPADASS TTTVPTTILQRWPKSASLPRHEESHAGTGDLAVP VHARLARLPWASRPPGSTGASWQDRTKGRKG*T WPPRCSWHAWAHRLARP*RTQG*KR*PGYDGLA RVKRTNGLQGLPWIQRGKGIQR*KG*PGSQRRKG FPRISWNVGAER*NGSKR*TWDSRTPRTHRKTR KTRQAGTERG*WSYGPTRQAWAFWSTWPSGAPR PPTCRTTYNGTQRGKRISRASRKMSLWTHYECE* PFLRGICVWAQFPASSCDFCGQQPGGA*EAEHP KRHCLPQRPEISVLQGQPWLAPHPADPFLPCGL HCRPARHLWGWAPAAWGGV*RR*QRCG*RLHPLS PCLLWRWSPA*GCGGL*RL*LWLPDMRDLSPWV IWRPAMHPVLLHRLHALPLLHLRAVRRRWAAPH RTGSSFSTVIKLAMSWPRLHQPLCDKNKDKFLLL RHAVNSV*LEEFRTTASCLNPRYWKTFHMPNLR PVPHRYHLAKQPATMLFLFWNVADPGRGLG*AL LDTHRPAGCFRMF*SLWGIWSTGLSAPDHRNVQA FSLTLGSPLSGLSRPMNHKAGKWARSLGVAHSF EPAKGPLDWPQPSPA*RGCGCPGKDLTVKKY*E PT*PRLAPSRG*RRGSSSQCFPKGRLLWFQNLWH HLDLLALSALCPLSQKCPSCPFPVQETHEDCVT CTSPHLGRLLVSPWSLGRYSPESPI*GQLQRAL SRQSVPGQSSTSLPPADP*QRPVAFLSCLHIA*P CPPVCLSSSLFNKIISRACL*AAFHHHFS*VGL SFILERHLQGEVHPHSRPQVEIAQFLPAGGTPL GFCTKLFNEPVSWGLSRLELGAWWTITWCL*VDP VSHR*HQPEGGVFRDRHMGSPVPFASTARPGEM LFSCCFHHHTGVSGWEIKK*RQFISP