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Sequences

Sequence NM_016613

GCATTAGAGTCAAGGATTACATTCTTCCTGGAGCCAAGCATGGGGCCAGCTGTAAACAAGCCGCATTTCTCCTTGGGGAGACTGATAATTTAAAAGGTTT GTTGTGTCAGAAACATTCCCAGCTTCATCACCAACCCTTTCCTTCCACCTCTGCCCACTGGAGACCACTTATATCCCGAAGCGGACGCGGCAGCTGAAGT CAGGAAACCATGCATCACATTAGCAGGAGCCAACTGCAGACTTTAAACTCCGTTCAACATGTGGATGCGGCAGAGAAATGACCTGTCCAGACAAGCCGGG GCAGCTCATAAACTGGTTCATCTGCTCCCTGTGCGTCCCGCGGGTGCGTAAGCTCTGGAGCAGCCGGCGTCCAAGGACCCGGAGAAACCTTCTGCTGGGC ACTGCGTGTGCCATCTACTTGGGCTTCCTGGTGAGCCAGGTGGGGAGGGCCTCTCTCCAGCATGGACAGGCGGCTGAGAAGGGGCCACATCGCAGCCGCG ACACCGCCGAGCCATCCTTCCCTGAGATACCCCTGGATGGTACCCTGGCCCCTCCAGAGTCCCAGGGCAATGGGTCCACTCTGCAGCCCAATGTGGTGTA CATTACCCTACGCTCCAAGCGCAGCAAGCCGGCCAATATCCGTGGCACCGTGAAGCCCAAGCGCAGGAAAAAGCATGCAGTGGCATCGGCTGCCCCAGGG CAGGAGGCTTTGGTCGGACCATCCCTTCAGCCGCAGGAAGCGGCAAGGGAAGCTGATGCTGTAGCACCTGGGTACGCTCAGGGAGCAAACCTGGTTAAGA TTGGAGAGCGACCCTGGAGGTTGGTGCGGGGTCCGGGAGTGCGAGCCGGGGGCCCAGACTTCCTGCAGCCCAGCTCCAGGGAGAGCAACATTAGGATCTA CAGCGAGAGCGCCCCCTCCTGGCTGAGCAAAGATGACATCCGAAGAATGCGACTCTTGGCGGACAGCGCAGTGGCAGGGCTCCGGCCTGTGTCCTCTAGG AGCGGAGCCCGTTTGCTGGTGCTGGAGGGGGGCGCACCTGGCGCTGTGCTCCGCTGTGGCCCTAGCCCCTGTGGGCTTCTCAAGCAGCCCTTGGACATGA GTGAGGTGTTTGCCTTCCACCTAGACAGGATCCTGGGGCTCAACAGGACCCTGCCGTCTGTGAGCAGGAAAGCAGAGTTCATCCAAGATGGCCGCCCATG CCCCATCATTCTTTGGGATGCATCTTTATCTTCAGCAAGTAATGACACCCATTCTTCTGTTAAGCTCACCTGGGGAACTTATCAGCAGTTGCTGAAACAG AAATGCTGGCAGAATGGCCGAGTACCCAAGCCTGAATCGGGTTGTACTGAAATACATCATCATGAGTGGTCCAAGATGGCACTCTTTGATTTTTTGTTAC AGATTTATAATCGCTTAGATACAAATTGCTGTGGATTCAGACCTCGCAAGGAAGATGCCTGTGTACAGAATGGATTGAGGCCAAAATGTGATGACCAAGG TTCTGCGGCTCTAGCACACATTATCCAGCGAAAGCATGACCCAAGGCATTTGGTTTTTATAGACAACAAGGGTTTCTTTGACAGGAGTGAAGATAACTTA AACTTCAAATTGTTAGAAGGCATCAAAGAGTTTCCAGCTTCTGCAGTTTCTGTTTTGAAGAGCCAGCACTTACGGCAGAAACTTCTTCAGTCTCTGTTTC TTGATAAAGTGTATTGGGAAAGTCAAGGAGGTAGACAAGGAATTGAAAAGCTTATCGATGTAATAGAACACAGAGCCAAAATTCTTATCACCTATATCAA TGCACACGGGGTCAAAGTATTACCTATGAATGAATGACAAAAGAATCTTCTGGCTAGGGTGTTAGATATATTTATGCATTTTTGGTTTTGTTTTTAAATC AAGCACATCAACCTCAAGCCCGTTTAGCAATGAGGCAGTGTAGATGAATACGTAAAATAAATGACTTTAACCAAGTAGCTATAATGGGACTTAGCACTGT ATGCATACTTAAAAAGGTTTTGAAAAACAAACTACTTGAGAAATATTTGTTTATATTTTTCTCTAACATCATGCTATGTGTCAGTCTGAACATCTGACAA CAGAAATTTCAGTTATTATTCTAGCTAAGTTTTGAAAACATTTGTCATGCTGTTTAATAGAAAACTGCAAACCAGAGACACTGACTCCATTAATAAACCA TATTTTGTGCCGTTTTGACTGTTCTGACCAAATACTAATGGGAACAATTCTTGACGTTTTTCTGTTGCTGATTGTTAACATAGAGCAGTCTCTACACTAC CCTGAGGCAACTCTACATTGGAACACTGAGGCTTACAGCCTGCAAGAGCATCAGAGCTGACCATACATTTAAACAGAAATGCTGGTTTATTTGCAAAATC ACCAGTATATTTTCTATTGTGTCTATAAAAAATCAGTCATTTAAGTACAAGAATCATATTTTCCATTCCTTTTTAGAAATTTATTTTGTTGTCCCTATGG AAATCATTCACATCTGACAATTTATATGTTAAAGAGTTTTACTCTCTCTATTTTGGTCCAATTTGTATCTAGTGGCTGAGAAATTAAATAATTCTAAAGT ATGAAGTTACCTATCTGAAAATGTACTTACAGAGTATCATTTTAAAATGGATGTCTCTTTAAAAATTTTGTTACTTTTACCAACAATGTAATATAATTTA TGTATATTTTATTAATAATAGTGAATTCCTTAAAATTTGTTCTATGTACTTATATTTAATTTGATTTAATGGTTACTGCCCAGATATTGAGAATTGGTTC AAATATTGAGTGTGTTTCAATATATTATCTGGCTTATTTCAACATGAGTAATATGAGCAAAATAAGTTAAAACCTGCGTCTGATCAATTTTCCTCATGAC TAGAACTAAAACAGTAAATTTGGACAATATTAAGCCTCAAATAATCATCTCCAAACTCCTTCTAACACTTTTTAAATCAGATTGGAAGACATGGACAAAT CAGGTTCATGTGTTGCATCTTTATGTCCTTTGCCAATATCCAAGATCATCACATATGGTAGATATTCACATGGAGTTTCAAATTCAGAATAGATTACCAT TACCTTCCTGCCCTTACACATCCTACTCCTTATTTAAAAGTTCTATTTGTGACTTTTCATTTCCTGAAAGTTTAAAAATACAATTTGAGAATGTTTATAA TACATTCTCTCCTGTCTTTTCACGGTTACGTCTGTTATTGCTGAAATACACCACATTTTCTTTGTTCTGGTCAAGGTTAACTCAATATCTGTGTGAAAGA GAACTACTAACAACGTTACAATAGAGGCTAGATTTGAAAAAAAAAATCTATAGATCTAATTGATACAATTGTAGAACAAAATGTCAAAATAATGTTTTAA GTATAAGAGAAGATGGACCAAGGAGAGAGAGATCATTTGAAAATCTAATTGTAGCTTTTCTAGGCTCACATTCATGTACTACTTTTAGCACCCTTATGGG CTGTGCTCGCCCCCTGGACAGTTGAGCTTTGGATTATCTTCCTCTTCAATTTTCCCTCTATTGACCCGAGTGTCTCCCTCTGCTTCTACAGATTTATAGT ACTCCTTGGCTCTTTTGAGTCTCCACTTTTACTCACTGTCTCTGGGATTTTTAAGATCCTTTTCTTCTCTTATAAATCATCCTCTTAATGAAAATTAGCC TAACAAAAGTTTGGAGACTGGAATCCTACTTTGAGCCACTGACTTGAAATAACTCTTTTGGCAAGTTGCCTGACATCCTGTCTTACCAAGGTGGCATATT TGCATTTTTACTGCTTAAAACATTTTTTTTTTTTTACCATCTTTATCCAAATTTATCATATTGATGGTAGGACTAACAGGCTTTTTAGAAGCTGGCTTTA ACTTTGAGTCTCAAGCTACAATGCTGTTGGGCAGCCTGGTCTTCCCACGTGAGGGTTTAACTTTGTTTATTTGCCTCCAGTTATTCCAAAATGCTTATTA AATGAAAGTCCCAGGAACATGTTTATTTTAGTCACCTTTGCTTTTTAACAATTTTGTTTTGTAATCAATGAGTAATTCATGATGAATTATTTTTGACTAA TGGATAGCCGAAGGCCAGGCTTTTAATTCTAATAGGTAATGTTCTTCTTTTGTCTTATTGAAACAATGAGAATACTCTGTGCATTTCAAATGCACTCCGA TTATGCTGTGGTTTTATTCACATAAGCACAATATGTGTTTTATTTATAACTTCATAACAAACTTATAATATAATAATTTACCTTAGCAGACATGCAAAAG CTTATTCTTGTGTGACTTACTTTCTTTAAGCTAATAATATAAAAATAAATATGTATCTTAAAAATCTATAATAAAACATTAGAAATTAAAGATATGTGCT TTTTATTTTGCAGATGAGTTCATTTGCTTTTGTAGATGTGTTTTCAGAGCTAGGTACAGAGGAATGTTTGCTACCTTTAGCGGTGAAAAAAGAAAGAGAG TCAAGAATTTTGTTGGATTGTGTTTGTGTGTGCATATATTTGATATCATCATTATATTTGTAATCTTTGGACTTGTAATCATAGCCTGTTTATTCTACTG TGCCATTAAATATACTTTACCTTATACATAACGAA

refseq_C4orf18.F1 (forward primer)
refseq_C4orf18.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

ALESRITFFLEPSMGPAVNKPHFSLGRLII*KV CCVRNIPSFITNPFLPPLPTGDHLYPEADAAAEV RKPCITLAGANCRL*TPFNMWMRQRNDLSRQAG AAHKLVHLLPVRPAGA*ALEQPASKDPEKPSAG HCVCHLLGLPGEPGGEGLSPAWTGG*EGATSQPR HRRAILP*DTPGWYPGPSRVPGQWVHSAAQCGV HYPTLQAQQAGQYPWHREAQAQEKACSGIGCPR AGGFGRTIPSAAGSGKGS*CCSTWVRSGSKPG*D WRATLEVGAGSGSASRGPRLPAAQLQGEQH*DL QRERPLLAEQR*HPKNATLGGQRSGRAPACVL* ERSPFAGAGGGRTWRCAPLWP*PLWASQAALGHE *GVCLPPRQDPGAQQDPAVCEQESRVHPRWPPM PHHSLGCIFIFSK**HPFFC*AHLGNLSAVAET EMLAEWPSTQA*IGLY*NTSS*VVQDGTL*FFVT DL*SLRYKLLWIQTSQGRCLCTEWIEAKM**PR FCGSSTHYPAKA*PKAFGFYRQQGFL*QE*R*L KLQIVRRHQRVSSFCSFCFEEPALTAETSSVSVS **SVLGKSRR*TRN*KAYRCNRTQSQNSYHLYQ CTRGQSITYE*MTKESSG*GVRYIYAFLVLFLN QAHQPQARLAMRQCR*IRKINDFNQVAIMGLSTV CILKKVLKNKLLEKYLFIFFSNIMLCVSLNI*Q QKFQLLF*LSFENICHAV**KTANQRH*LH**T IFCAVLTVLTKY*WEQFLTFFCC*LLT*SSLYTT LRQLYIGTLRLTACKSIRADHTFKQKCWFICKI TSIFSIVSIKNQSFKYKNHIFHSFLEIYFVVPM EIIHI*QFIC*RVLLSLFWSNLYLVAEKLNNSKV *SYLSENVLTEYHFKMDVSLKILLLLPTM*YNL CIFY****IP*NLFYVLIFNLI*WLLPRY*ELV QILSVFQYIIWLIST*VI*AK*VKTCV*SIFLMT RTKTVNLDNIKPQIIISKLLLTLFKSDWKTWTN QVHVLHLYVLCQYPRSSHMVDIHMEFQIQNRLP LPSCPYTSYSLFKSSICDFSFPESLKIQFENVYN TFSPVFSRLRLLLLKYTTFSLFWSRLTQYLCER ELLTTLQ*RLDLKKKIYRSN*YNCRTKCQNNVL SIREDGPRRERSFENLIVAFLGSHSCTTFSTLMG CARPLDS*ALDYLPLQFSLY*PECLPLLLQIYS TPWLF*VSTFTHCLWDF*DPFLLL*IILLMKIS LTKVWRLESYFEPLT*NNSFGKLPDILSYQGGIF AFLLLKTFFFFYHLYPNLSY*W*D*QAF*KLAL TLSLKLQCCWAAWSSHVRV*LCLFASSYSKMLI K*KSQEHVYFSHLCFLTILFCNQ*VIHDELFLTN G*PKARLLILIGNVLLLSY*NNENTLCISNALR LCCGFIHISTICVLFITS*QTYNIIIYLSRHAK AYSCVTYFL*ANNIKINMYLKNL**NIRN*RYVL FILQMSSFAFVDVFSELGTEECLLPLAVKKERE SRILLDCVCVCIYLISSLYL*SLDL*S*PVYST VPLNILYLIHNE

Frame 1

H*SQGLHSSWSQAWGQL*TSRISPWGD**FKRF VVSETFPASSPTLSFHLCPLETTYIPKRTRQLK SGNHASH*QEPTADFKLRSTCGCGREMTCPDKPG QLINWFICSLCVPRVRKLWSSRRPRTRRNLLLG TACAIYLGFLVSQVGRASLQHGQAAEKGPHRSR DTAEPSFPEIPLDGTLAPPESQGNGSTLQPNVVY ITLRSKRSKPANIRGTVKPKRRKKHAVASAAPG QEALVGPSLQPQEAAREADAVAPGYAQGANLVK IGERPWRLVRGPGVRAGGPDFLQPSSRESNIRIY SESAPSWLSKDDIRRMRLLADSAVAGLRPVSSR SGARLLVLEGGAPGAVLRCGPSPCGLLKQPLDM SEVFAFHLDRILGLNRTLPSVSRKAEFIQDGRPC PIILWDASLSSASNDTHSSVKLTWGTYQQLLKQ KCWQNGRVPKPESGCTEIHHHEWSKMALFDFLL QIYNRLDTNCCGFRPRKEDACVQNGLRPKCDDQG SAALAHIIQRKHDPRHLVFIDNKGFFDRSEDNL NFKLLEGIKEFPASAVSVLKSQHLRQKLLQSLF LDKVYWESQGGRQGIEKLIDVIEHRAKILITYIN AHGVKVLPMNE*QKNLLARVLDIFMHFWFCF*I KHINLKPV*Q*GSVDEYVK*MTLTK*L*WDLAL YAYLKRF*KTNYLRNICLYFSLTSCYVSV*TSDN RNFSYYSS*VLKTFVMLFNRKLQTRDTDSINKP YFVPF*LF*PNTNGNNS*RFSVADC*HRAVSTL P*GNSTLEH*GLQPARASELTIHLNRNAGLFAKS PVYFLLCL*KISHLSTRIIFSIPF*KFILLSLW KSFTSDNLYVKEFYSLYFGPICI*WLRN*IILK YEVTYLKMYLQSIILKWMSL*KFCYFYQQCNIIY VYFINNSEFLKICSMYLYLI*FNGYCPDIENWF KY*VCFNILSGLFQHE*YEQNKLKPASDQFSS* LELKQ*IWTILSLK*SSPNSF*HFLNQIGRHGQI RFMCCIFMSFANIQDHHIW*IFTWSFKFRIDYH YLPALTHPTPYLKVLFVTFHFLKV*KYNLRMFI IHSLLSFHGYVCYC*NTPHFLCSGQG*LNICVKE NY*QRYNRG*I*KKKSIDLIDTIVEQNVKIMF* V*EKMDQGERDHLKI*L*LF*AHIHVLLLAPLW AVLAPWTVELWIIFLFNFPSIDPSVSLCFYRFIV LLGSFESPLLLTVSGIFKILFFSYKSSS**KLA *QKFGDWNPTLSH*LEITLLASCLTSCLTKVAY LHFYCLKHFFFFTIFIQIYHIDGRTNRLFRSWL* L*VSSYNAVGQPGLPT*GFNFVYLPPVIPKCLL NESPRNMFILVTFAF*QFCFVINE*FMMNYF*L MDSRRPGF*F**VMFFFCLIETMRILCAFQMHSD YAVVLFT*AQYVFYL*LHNKLII**FTLADMQK LILV*LTFFKLII*K*ICILKIYNKTLEIKDMC FLFCR*VHLLL*MCFQS*VQRNVCYL*R*KKKES QEFCWIVFVCAYI*YHHYICNLWTCNHSLFILL CH*IYFTLYIT

Frame 2

IRVKDYILPGAKHGASCKQAAFLLGETDNLKGL LCQKHSQLHHQPFPSTSAHWRPLISRSGRGS*S QETMHHISRSQLQTLNSVQHVDAAEK*PVQTSR GSS*TGSSAPCASRGCVSSGAAGVQGPGETFCWA LRVPSTWASW*ARWGGPLSSMDRRLRRGHIAAA TPPSHPSLRYPWMVPWPLQSPRAMGPLCSPMWC TLPYAPSAASRPISVAP*SPSAGKSMQWHRLPQG RRLWSDHPFSRRKRQGKLML*HLGTLREQTWLR LESDPGGWCGVRECEPGAQTSCSPAPGRATLGS TARAPPPG*AKMTSEECDSWRTAQWQGSGLCPLG AEPVCWCWRGAHLALCSAVALAPVGFSSSPWT* VRCLPST*TGSWGSTGPCRL*AGKQSSSKMAAH APSFFGMHLYLQQVMTPILLLSSPGELISSC*NR NAGRMAEYPSLNRVVLKYIIMSGPRWHSLIFCY RFIIA*IQIAVDSDLARKMPVYRMD*GQNVMTK VLRL*HTLSSESMTQGIWFL*TTRVSLTGVKIT* TSNC*KASKSFQLLQFLF*RASTYGRNFFSLCF LIKCIGKVKEVDKELKSLSM**NTEPKFLSPIS MHTGSKYYL*MNDKRIFWLGC*IYLCIFGFVFKS STSTSSPFSNEAV*MNT*NK*L*PSSYNGT*HC MHT*KGFEKQTT*EIFVYIFL*HHAMCQSEHLT TEISVIILAKF*KHLSCCLIENCKPETLTPLINH ILCRFDCSDQILMGTILDVFLLLIVNIEQSLHY PEATLHWNTEAYSLQEHQS*PYI*TEMLVYLQN HQYIFYCVYKKSVI*VQESYFPFLFRNLFCCPYG NHSHLTIYMLKSFTLSILVQFVSSG*EIK*F*S MKLPI*KCTYRVSF*NGCLFKNFVTFTNNVI*F MYILLIIVNSLKFVLCTYI*FDLMVTAQILRIGS NIECVSIYYLAYFNMSNMSKIS*NLRLINFPHD *N*NSKFGQY*ASNNHLQTPSNTF*IRLEDMDK SGSCVASLCPLPISKIITYGRYSHGVSNSE*ITI TFLPLHILLLI*KFYL*LFIS*KFKNTI*ECL* YILSCLFTVTSVIAEIHHIFFVLVKVNSISV*K RTTNNVTIEARFEKKNL*I*LIQL*NKMSK*CFK YKRRWTKEREII*KSNCSFSRLTFMYYF*HPYG LCSPPGQLSFGLSSSSIFPLLTRVSPSASTDL* YSLALLSLHFYSLSLGFLRSFSSLINHPLNEN*P NKSLETGILL*ATDLK*LFWQVA*HPVLPRWHI CIFTA*NIFFFLPSLSKFIILMVGLTGFLEAGF NFESQATMLLGSLVFPREGLTLFICLQLFQNAY* MKVPGTCLF*SPLLFNNFVL*SMSNS**IIFD* WIAEGQAFNSNR*CSSFVLLKQ*EYSVHFKCTP IMLWFYSHKHNMCFIYNFITNL*YNNLP*QTCKS LFLCDLLSLS**YKNKYVS*KSIIKH*KLKICA FYFADEFICFCRCVFRARYRGMFATFSGEKRKR VKNFVGLCLCVHIFDIIIIFVIFGLVIIACLFYC AIKYTLPYT*R

Sequence NM_001031700

GTGAACTGTTGCACCGTGCAATTGCACACTATAAATGTCTTTCCTTATCTGTGTGTACTCTTATCTCACTGTTCTATTTTTTCTCCTCATTTATATTAAC TCTTTCTTACCTTTTTTTCTGAACTTCTAGGCCTTCTCTTTCCAGAACTGGTGGAAGACAAATGAAACGGCCAAGATGGTAAGAAACAAGCCGCATTTCT CCTTGGGGAGACTGATAATTTAAAAGGTTTGTTGTGTCAGAAACATTCCCAGCTTCATCACCAACCCTTTCCTTCCACCTCTGCCCACTGGAGACCACTT ATATCCCGAAGCGGACGCGGCAGCTGAAGTCAGGAAACCATGCATCACATTAGCAGGAGCCAACTGCAGACTTTAAACTCCGTTCAACATGTGGATGCGG CAGAGAAATGACCTGTCCAGACAAGCCGGGGCAGCTCATAAACTGGTTCATCTGCTCCCTGTGCGTCCCGCGGGTGCGTAAGCTCTGGAGCAGCCGGCGT CCAAGGACCCGGAGAAACCTTCTGCTGGGCACTGCGTGTGCCATCTACTTGGGCTTCCTGGTGAGCCAGGTGGGGAGGGCCTCTCTCCAGCATGGACAGG CGGCTGAGAAGGGGCCACATCGCAGCCGCGACACCGCCGAGCCATCCTTCCCTGAGATACCCCTGGATGGTACCCTGGCCCCTCCAGAGTCCCAGGGCAA TGGGTCCACTCTGCAGCCCAATGTGGTGTACATTACCCTACGCTCCAAGCGCAGCAAGCCGGCCAATATCCGTGGCACCGTGAAGCCCAAGCGCAGGAAA AAGCATGCAGTGGCATCGGCTGCCCCAGGGCAGGAGGCTTTGGTCGGACCATCCCTTCAGCCGCAGGAAGCGGCAAGGGAAGCTGATGCTGTAGCACCTG GGTACGCTCAGGGAGCAAACCTGGTTAAGATTGGAGAGCGACCCTGGAGGTTGGTGCGGGGTCCGGGAGTGCGAGCCGGGGGCCCAGACTTCCTGCAGCC CAGCTCCAGGGAGAGCAACATTAGGATCTACAGCGAGAGCGCCCCCTCCTGGCTGAGCAAAGATGACATCCGAAGAATGCGACTCTTGGCGGACAGCGCA GTGGCAGGGCTCCGGCCTGTGTCCTCTAGGAGCGGAGCCCGTTTGCTGGTGCTGGAGGGGGGCGCACCTGGCGCTGTGCTCCGCTGTGGCCCTAGCCCCT GTGGGCTTCTCAAGCAGCCCTTGGACATGAGTGAGGTGTTTGCCTTCCACCTAGACAGGATCCTGGGGCTCAACAGGACCCTGCCGTCTGTGAGCAGGAA AGCAGAGTTCATCCAAGCAGCAGCAGCAGCGTGTCTTTCCATGCGCTTGGCATTCTTTATTTTCCCAGCCTGGGAGGATATGAGAGTTCCAGGGAAATGC TGTATTGGACATGCAAGACTCACCTGGGGAACTTATCAGCAGTTGCTGAAACAGAAATGCTGGCAGAATGGCCGAGTACCCAAGCCTGAATCGGGTTGTA CTGAAATACATCATCATGAGTGGTCCAAGATGGCACTCTTTGATTTTTTGTTACAGATTTATAATCGCTTAGATACAAATTGCTGTGGATTCAGACCTCG CAAGGAAGATGCCTGTGTACAGAATGGATTGAGGCCAAAATGTGATGACCAAGGTTCTGCGGCTCTAGCACACATTATCCAGCGAAAGCATGACCCAAGG CATTTGGTTTTTATAGACAACAAGGGTTTCTTTGACAGGAGTGAAGATAACTTAAACTTCAAATTGTTAGAAGGCATCAAAGAGTTTCCAGCTTCTGCAG TTTCTGTTTTGAAGAGCCAGCACTTACGGCAGAAACTTCTTCAGTCTCTGTTTCTTGATAAAGTGTATTGGGAAAGTCAAGGAGGTAGACAAGGAATTGA AAAGCTTATCGATGTAATAGAACACAGAGCCAAAATTCTTATCACCTATATCAATGCACACGGGGTCAAAGTATTACCTATGAATGAATGACAAAAGAAT CTTCTGGCTAGGGTGTTAGATATATTTATGCATTTTTGGTTTTGTTTTTAAATCAAGCACATCAACCTCAAGCCCGTTTAGCAATGAGGCAGTGTAGATG AATACGTAAAATAAATGACTTTAACCAAGTAGCTATAATGGGACTTAGCACTGTATGCATACTTAAAAAGGTTTTGAAAAACAAACTACTTGAGAAATAT TTGTTTATATTTTTCTCTAACATCATGCTATGTGTCAGTCTGAACATCTGACAACAGAAATTTCAGTTATTATTCTAGCTAAGTTTTGAAAACATTTGTC ATGCTGTTTAATAGAAAACTGCAAACCAGAGACACTGACTCCATTAATAAACCATATTTTGTGCCGTTTTGACTGTTCTGACCAAATACTAATGGGAACA ATTCTTGACGTTTTTCTGTTGCTGATTGTTAACATAGAGCAGTCTCTACACTACCCTGAGGCAACTCTACATTGGAACACTGAGGCTTACAGCCTGCAAG AGCATCAGAGCTGACCATACATTTAAACAGAAATGCTGGTTTATTTGCAAAATCACCAGTATATTTTCTATTGTGTCTATAAAAAATCAGTCATTTAAGT ACAAGAATCATATTTTCCATTCCTTTTTAGAAATTTATTTTGTTGTCCCTATGGAAATCATTCACATCTGACAATTTATATGTTAAAGAGTTTTACTCTC TCTATTTTGGTCCAATTTGTATCTAGTGGCTGAGAAATTAAATAATTCTAAAGTATGAAGTTACCTATCTGAAAATGTACTTACAGAGTATCATTTTAAA ATGGATGTCTCTTTAAAAATTTTGTTACTTTTACCAACAATGTAATATAATTTATGTATATTTTATTAATAATAGTGAATTCCTTAAAATTTGTTCTATG TACTTATATTTAATTTGATTTAATGGTTACTGCCCAGATATTGAGAATTGGTTCAAATATTGAGTGTGTTTCAATATATTATCTGGCTTATTTCAACATG AGTAATATGAGCAAAATAAGTTAAAACCTGCGTCTGATCAATTTTCCTCATGACTAGAACTAAAACAGTAAATTTGGACAATATTAAGCCTCAAATAATC ATCTCCAAACTCCTTCTAACACTTTTTAAATCAGATTGGAAGACATGGACAAATCAGGTTCATGTGTTGCATCTTTATGTCCTTTGCCAATATCCAAGAT CATCACATATGGTAGATATTCACATGGAGTTTCAAATTCAGAATAGATTACCATTACCTTCCTGCCCTTACACATCCTACTCCTTATTTAAAAGTTCTAT TTGTGACTTTTCATTTCCTGAAAGTTTAAAAATACAATTTGAGAATGTTTATAATACATTCTCTCCTGTCTTTTCACGGTTACGTCTGTTATTGCTGAAA TACACCACATTTTCTTTGTTCTGGTCAAGGTTAACTCAATATCTGTGTGAAAGAGAACTACTAACAACGTTACAATAGAGGCTAGATTTGAAAAAAAAAA TCTATAGATCTAATTGATACAATTGTAGAACAAAATGTCAAAATAATGTTTTAAGTATAAGAGAAGATGGACCAAGGAGAGAGAGATCATTTGAAAATCT AATTGTAGCTTTTCTAGGCTCACATTCATGTACTACTTTTAGCACCCTTATGGGCTGTGCTCGCCCCCTGGACAGTTGAGCTTTGGATTATCTTCCTCTT CAATTTTCCCTCTATTGACCCGAGTGTCTCCCTCTGCTTCTACAGATTTATAGTACTCCTTGGCTCTTTTGAGTCTCCACTTTTACTCACTGTCTCTGGG ATTTTTAAGATCCTTTTCTTCTCTTATAAATCATCCTCTTAATGAAAATTAGCCTAACAAAAGTTTGGAGACTGGAATCCTACTTTGAGCCACTGACTTG AAATAACTCTTTTGGCAAGTTGCCTGACATCCTGTCTTACCAAGGTGGCATATTTGCATTTTTACTGCTTAAAACATTTTTTTTTTTTTACCATCTTTAT CCAAATTTATCATATTGATGGTAGGACTAACAGGCTTTTTAGAAGCTGGCTTTAACTTTGAGTCTCAAGCTACAATGCTGTTGGGCAGCCTGGTCTTCCC ACGTGAGGGTTTAACTTTGTTTATTTGCCTCCAGTTATTCCAAAATGCTTATTAAATGAAAGTCCCAGGAACATGTTTATTTTAGTCACCTTTGCTTTTT AACAATTTTGTTTTGTAATCAATGAGTAATTCATGATGAATTATTTTTGACTAATGGATAGCCGAAGGCCAGGCTTTTAATTCTAATAGGTAATGTTCTT CTTTTGTCTTATTGAAACAATGAGAATACTCTGTGCATTTCAAATGCACTCCGATTATGCTGTGGTTTTATTCACATAAGCACAATATGTGTTTTATTTA TAACTTCATAACAAACTTATAATATAATAATTTACCTTAGCAGACATGCAAAAGCTTATTCTTGTGTGACTTACTTTCTTTAAGCTAATAATATAAAAAT AAATATGTATCTTAAAAATCTATAATAAAACATTAGAAATTAAAGATATGTGCTTTTTATTTTGCAGATGAGTTCATTTGCTTTTGTAGATGTGTTTTCA GAGCTAGGTACAGAGGAATGTTTGCTACCTTTAGCGGTGAAAAAAGAAAGAGAGTCAAGAATTTTGTTGGATTGTGTTTGTGTGTGCATATATTTGATAT CATCATTATATTTGTAATCTTTGGACTTGTAATCATAGCCTGTTTATTCTACTGTGCCATTAAATATACTTTACCTTATACATAACGAA

refseq_C4orf18.F1 (forward primer)
refseq_C4orf18.R1 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

VNCCTVQLHTINVFPYLCVLLSHCSIFSPHLY* LFLTFFSELLGLLFPELVEDK*NGQDGKKQAAFL LGETDNLKGLLCQKHSQLHHQPFPSTSAHWRPL ISRSGRGS*SQETMHHISRSQLQTLNSVQHVDA AEK*PVQTSRGSS*TGSSAPCASRGCVSSGAAGV QGPGETFCWALRVPSTWASW*ARWGGPLSSMDR RLRRGHIAAATPPSHPSLRYPWMVPWPLQSPRA MGPLCSPMWCTLPYAPSAASRPISVAP*SPSAGK SMQWHRLPQGRRLWSDHPFSRRKRQGKLML*HL GTLREQTWLRLESDPGGWCGVRECEPGAQTSCS PAPGRATLGSTARAPPPG*AKMTSEECDSWRTAQ WQGSGLCPLGAEPVCWCWRGAHLALCSAVALAP VGFSSSPWT*VRCLPST*TGSWGSTGPCRL*AG KQSSSKQQQQRVFPCAWHSLFSQPGRI*EFQGNA VLDMQDSPGELISSC*NRNAGRMAEYPSLNRVV LKYIIMSGPRWHSLIFCYRFIIA*IQIAVDSDL ARKMPVYRMD*GQNVMTKVLRL*HTLSSESMTQG IWFL*TTRVSLTGVKIT*TSNC*KASKSFQLLQ FLF*RASTYGRNFFSLCFLIKCIGKVKEVDKEL KSLSM**NTEPKFLSPISMHTGSKYYL*MNDKRI FWLGC*IYLCIFGFVFKSSTSTSSPFSNEAV*M NT*NK*L*PSSYNGT*HCMHT*KGFEKQTT*EI FVYIFL*HHAMCQSEHLTTEISVIILAKF*KHLS CCLIENCKPETLTPLINHILCRFDCSDQILMGT ILDVFLLLIVNIEQSLHYPEATLHWNTEAYSLQ EHQS*PYI*TEMLVYLQNHQYIFYCVYKKSVI*V QESYFPFLFRNLFCCPYGNHSHLTIYMLKSFTL SILVQFVSSG*EIK*F*SMKLPI*KCTYRVSF* NGCLFKNFVTFTNNVI*FMYILLIIVNSLKFVLC TYI*FDLMVTAQILRIGSNIECVSIYYLAYFNM SNMSKIS*NLRLINFPHD*N*NSKFGQY*ASNN HLQTPSNTF*IRLEDMDKSGSCVASLCPLPISKI ITYGRYSHGVSNSE*ITITFLPLHILLLI*KFY L*LFIS*KFKNTI*ECL*YILSCLFTVTSVIAE IHHIFFVLVKVNSISV*KRTTNNVTIEARFEKKN L*I*LIQL*NKMSK*CFKYKRRWTKEREII*KS NCSFSRLTFMYYF*HPYGLCSPPGQLSFGLSSS SIFPLLTRVSPSASTDL*YSLALLSLHFYSLSLG FLRSFSSLINHPLNEN*PNKSLETGILL*ATDL K*LFWQVA*HPVLPRWHICIFTA*NIFFFLPSL SKFIILMVGLTGFLEAGFNFESQATMLLGSLVFP REGLTLFICLQLFQNAY*MKVPGTCLF*SPLLF NNFVL*SMSNS**IIFD*WIAEGQAFNSNR*CS SFVLLKQ*EYSVHFKCTPIMLWFYSHKHNMCFIY NFITNL*YNNLP*QTCKSLFLCDLLSLS**YKN KYVS*KSIIKH*KLKICAFYFADEFICFCRCVF RARYRGMFATFSGEKRKRVKNFVGLCLCVHIFDI IIIFVIFGLVIIACLFYCAIKYTLPYT*R

Frame 1

*TVAPCNCTL*MSFLICVYSYLTVLFFLLIYIN SFLPFFLNF*AFSFQNWWKTNETAKMVRNKPHF SLGRLII*KVCCVRNIPSFITNPFLPPLPTGDHL YPEADAAAEVRKPCITLAGANCRL*TPFNMWMR QRNDLSRQAGAAHKLVHLLPVRPAGA*ALEQPA SKDPEKPSAGHCVCHLLGLPGEPGGEGLSPAWTG G*EGATSQPRHRRAILP*DTPGWYPGPSRVPGQ WVHSAAQCGVHYPTLQAQQAGQYPWHREAQAQE KACSGIGCPRAGGFGRTIPSAAGSGKGS*CCSTW VRSGSKPG*DWRATLEVGAGSGSASRGPRLPAA QLQGEQH*DLQRERPLLAEQR*HPKNATLGGQR SGRAPACVL*ERSPFAGAGGGRTWRCAPLWP*PL WASQAALGHE*GVCLPPRQDPGAQQDPAVCEQE SRVHPSSSSSVSFHALGILYFPSLGGYESSREM LYWTCKTHLGNLSAVAETEMLAEWPSTQA*IGLY *NTSS*VVQDGTL*FFVTDL*SLRYKLLWIQTS QGRCLCTEWIEAKM**PRFCGSSTHYPAKA*PK AFGFYRQQGFL*QE*R*LKLQIVRRHQRVSSFCS FCFEEPALTAETSSVSVS**SVLGKSRR*TRN* KAYRCNRTQSQNSYHLYQCTRGQSITYE*MTKE SSG*GVRYIYAFLVLFLNQAHQPQARLAMRQCR* IRKINDFNQVAIMGLSTVCILKKVLKNKLLEKY LFIFFSNIMLCVSLNI*QQKFQLLF*LSFENIC HAV**KTANQRH*LH**TIFCAVLTVLTKY*WEQ FLTFFCC*LLT*SSLYTTLRQLYIGTLRLTACK SIRADHTFKQKCWFICKITSIFSIVSIKNQSFK YKNHIFHSFLEIYFVVPMEIIHI*QFIC*RVLLS LFWSNLYLVAEKLNNSKV*SYLSENVLTEYHFK MDVSLKILLLLPTM*YNLCIFY****IP*NLFY VLIFNLI*WLLPRY*ELVQILSVFQYIIWLIST* VI*AK*VKTCV*SIFLMTRTKTVNLDNIKPQII ISKLLLTLFKSDWKTWTNQVHVLHLYVLCQYPR SSHMVDIHMEFQIQNRLPLPSCPYTSYSLFKSSI CDFSFPESLKIQFENVYNTFSPVFSRLRLLLLK YTTFSLFWSRLTQYLCERELLTTLQ*RLDLKKK IYRSN*YNCRTKCQNNVLSIREDGPRRERSFENL IVAFLGSHSCTTFSTLMGCARPLDS*ALDYLPL QFSLY*PECLPLLLQIYSTPWLF*VSTFTHCLW DF*DPFLLL*IILLMKISLTKVWRLESYFEPLT* NNSFGKLPDILSYQGGIFAFLLLKTFFFFYHLY PNLSY*W*D*QAF*KLALTLSLKLQCCWAAWSS HVRV*LCLFASSYSKMLIK*KSQEHVYFSHLCFL TILFCNQ*VIHDELFLTNG*PKARLLILIGNVL LLSY*NNENTLCISNALRLCCGFIHISTICVLF ITS*QTYNIIIYLSRHAKAYSCVTYFL*ANNIKI NMYLKNL**NIRN*RYVLFILQMSSFAFVDVFS ELGTEECLLPLAVKKERESRILLDCVCVCIYLI SSLYL*SLDL*S*PVYSTVPLNILYLIHNE

Frame 2

ELLHRAIAHYKCLSLSVCTLISLFYFFSSFILT LSYLFF*TSRPSLSRTGGRQMKRPRW*ETSRIS PWGD**FKRFVVSETFPASSPTLSFHLCPLETT YIPKRTRQLKSGNHASH*QEPTADFKLRSTCGCG REMTCPDKPGQLINWFICSLCVPRVRKLWSSRR PRTRRNLLLGTACAIYLGFLVSQVGRASLQHGQ AAEKGPHRSRDTAEPSFPEIPLDGTLAPPESQGN GSTLQPNVVYITLRSKRSKPANIRGTVKPKRRK KHAVASAAPGQEALVGPSLQPQEAAREADAVAP GYAQGANLVKIGERPWRLVRGPGVRAGGPDFLQP SSRESNIRIYSESAPSWLSKDDIRRMRLLADSA VAGLRPVSSRSGARLLVLEGGAPGAVLRCGPSP CGLLKQPLDMSEVFAFHLDRILGLNRTLPSVSRK AEFIQAAAAACLSMRLAFFIFPAWEDMRVPGKC CIGHARLTWGTYQQLLKQKCWQNGRVPKPESGC TEIHHHEWSKMALFDFLLQIYNRLDTNCCGFRPR KEDACVQNGLRPKCDDQGSAALAHIIQRKHDPR HLVFIDNKGFFDRSEDNLNFKLLEGIKEFPASA VSVLKSQHLRQKLLQSLFLDKVYWESQGGRQGIE KLIDVIEHRAKILITYINAHGVKVLPMNE*QKN LLARVLDIFMHFWFCF*IKHINLKPV*Q*GSVD EYVK*MTLTK*L*WDLALYAYLKRF*KTNYLRNI CLYFSLTSCYVSV*TSDNRNFSYYSS*VLKTFV MLFNRKLQTRDTDSINKPYFVPF*LF*PNTNGN NS*RFSVADC*HRAVSTLP*GNSTLEH*GLQPAR ASELTIHLNRNAGLFAKSPVYFLLCL*KISHLS TRIIFSIPF*KFILLSLWKSFTSDNLYVKEFYS LYFGPICI*WLRN*IILKYEVTYLKMYLQSIILK WMSL*KFCYFYQQCNIIYVYFINNSEFLKICSM YLYLI*FNGYCPDIENWFKY*VCFNILSGLFQH E*YEQNKLKPASDQFSS*LELKQ*IWTILSLK*S SPNSF*HFLNQIGRHGQIRFMCCIFMSFANIQD HHIW*IFTWSFKFRIDYHYLPALTHPTPYLKVL FVTFHFLKV*KYNLRMFIIHSLLSFHGYVCYC*N TPHFLCSGQG*LNICVKENY*QRYNRG*I*KKK SIDLIDTIVEQNVKIMF*V*EKMDQGERDHLKI *L*LF*AHIHVLLLAPLWAVLAPWTVELWIIFLF NFPSIDPSVSLCFYRFIVLLGSFESPLLLTVSG IFKILFFSYKSSS**KLA*QKFGDWNPTLSH*L EITLLASCLTSCLTKVAYLHFYCLKHFFFFTIFI QIYHIDGRTNRLFRSWL*L*VSSYNAVGQPGLP T*GFNFVYLPPVIPKCLLNESPRNMFILVTFAF *QFCFVINE*FMMNYF*LMDSRRPGF*F**VMFF FCLIETMRILCAFQMHSDYAVVLFT*AQYVFYL *LHNKLII**FTLADMQKLILV*LTFFKLII*K *ICILKIYNKTLEIKDMCFLFCR*VHLLL*MCFQ S*VQRNVCYL*R*KKKESQEFCWIVFVCAYI*Y HHYICNLWTCNHSLFILLCH*IYFTLYIT