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Sequences

Sequence NM_080799

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGTCAACCAGGAACTAGAGGTTTCCCTGG
ATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCAAAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGA
CAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCCTAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGC
TGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACC
AGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAG
GGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCG
TGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGA
GAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGAT
GGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAG
GGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAAT
TGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCA
GGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGG
GGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCC
AGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAA
AAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAG
CGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAA
GGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGC
GAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACA
GTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTT
CCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTAT
CGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCA
TGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F6 (forward primer)
  • refseq_COL13A1.R6 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWVNQELEVSL
DFRVPLGWTANRATQDQRATWV*RVPQDSRDPRD
KKEKRVSVESTHTGSA*AACQQLCAPAK*LP*S
CCLSSIQCDWLHPRS*KGGRSRANRARPASKVH
QGPQAPLDQVDLWGTQDCQGLWGHLAYLGLLDQR
ETQGSRATTAGRENGACQGCQASMEPRGRPELP
WLG*RVSQGSQEPRVRRGLKAPLGFLASWGRRE
RKAMLATPLEEAEGNLALQGSLGPQGQREKQVSM
ARLAPQGSQETRGSVEQLENRDQMAPRAPRENQ
GKERWWITMETSMRLSRRSGRWP*WGLLVFLGK
LAHLELQGFQARRGRLDCQALQDTMGKRDLAVNQ
ETWALLVPKAPQERMDLQE*REKTGTQGAQERR
GKKGRQDKQAHRERKEKPGRRAIQEQRFLGCQG
QRGLPDLRGSKVFLDQRGKQD*MEQKERKASREK
KETVVPWDYPELQVWTAGLGHRVLQDQLEFQAQ
RDQRARGAAKETLG*QDQREQLGFLVYMDHPGT
RETGGRGGRKALEGLKGIRETKERLD*MPPAHWA
KMAYQSKAAGTSDASNLGLACVCVCT*NIYFYT
VFTF*KCQKYDASYRLLKKKEKPAYFVQKISTS
SLLFTRCFV*AYVSNTFFVWS*CLHDICAHLLSI
EA**IIVSWCQRGPARTEVLASSCVNSHKCRGP
*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGSTRN*RFPW
ISGSHWAGRQTGPPRTKGRHGSDGSPRTAGTPG
TKRRKGSVWRVPTPGVPKQHASSSALQPNNCPEA
AASPQFSATGSTPGHKKADVPGRTEPGQHPRST
RAPRPPWTKWTSGAPRTARAYGATWLTWASWTK
GRPRDPGLPRPEGRTGHARDARQAWSQGGARNCR
GWDEG*ARDPRNQG*EGG*RLPWASWPPGAEGR
ERRCWQLHWRRQRGTWPSRAPWAPRAKGRSRCR
WPGWPPRAARRQGGAWSSWRTGTRWPQGLQGRTR
ERRDGGLQWKHQ*GSPGDPDAGLDGASWSSWAN
WPTWSSRDSRPEGGDWTARPSRTRWGKGTSR*T
RRHGPSWSPRPPRKGWTSRSEGRKRAPREPRREG
GKRGDRTSRLTGRERRSRGEGQSRSRGSWAARA
RGASRTSGAPRCSWTKGGSRTRWSKRRERLPGR
KRRPWSPGTTRSFRFGRQAWATGYSRTNWSSRPS
GTKGREGQQRRPWDDRTNGSSWASWFTWTTRGQ
GKPGGEGEERL*RA*RG*GRPRSAWIRCPLPIG
RRWLTSPRLLEQVMPLTLDWPVCVFVHRIFIFIQ
FSLFENARSMMHLTDY*KRKKNLHILYRKYQPL
PFCLQDVLYKPMSLIHFLFGRNVCMIFVHIY*V
SKLNKLLCPGAKGGQPELRCWLAHV*IHINVEVH
DIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGQPGTRGFPG
FPGPIGLDGKPGHPGPKGDMGLTGPPGQPGPQG
QKGEKGQCGEYPHRECLSSMPAALRSSQIIALK
LLPLLNSVRLAPPPVIKRRTFQGEQSQASIQGPP
GPPGPPGPSGPLGHPGLPGPMGPPGLPGPPGPK
GDPGIQGYHGRKGERGMPGMPGKHGAKGAPGIA
VAGMKGEPGIPGTKGEKGAEGSPGLPGLLGQKGE
KGDAGNSIGGGRGEPGPPGLPGPPGPKGEAGVD
GQVGPPGQPGDKGERGAAGEQGPDGPKGSKGEP
GKGEMVDYNGNINEALQEIRTLALMGPPGLPGQI
GPPGAPGIPGQKGEIGLPGPPGHDGEKGPRGKP
GDMGPPGPQGPPGKDGPPGVKGENGHPGSPGEK
GEKGETGQAGSPGEKGEAGEKGNPGAEVPGLPGP
EGPPGPPGLQGVPGPKGEAGLDGAKGEKGFQGE
KGDRGPLGLPGASGLDGRPGPPGTPGPIGVPGP
AGPKGERGSKGDPGMTGPTGAAGLPGLHGPPGDK
GNRGERGKKGSRGPKGDKGDQGAPGLDAPCPLG
EDGLPVQGCWNK*CL*PWIGLCVCLYIEYLFLY
SFHFLKMPEV*CILQIIKKERKTCIFCTENINLF
PFVYKMFCISLCL*YIFCLVVMSA*YLCTFIKY
RSLINYCVLVPKGASQN*GAG*LMCEFT*M*RS
MIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080801

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCC
CCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATC
CAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGG
GTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGG
CAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCC
CCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGG
TGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGC
TCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCT
CCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGA
CAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGG
ACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGT
CCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCG
AGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGA
GAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCA
GTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGA
AAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAA
GATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAAT
TATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGC
TAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F6 (forward primer)
  • refseq_COL13A1.R6 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*RANRARPASKVHQGPQA
PLDQVDLWGTQDCQGLWGHLAYLGLLDQRETQGS
RATTAGRENGACQGCQASMEPRGRPELPWLG*R
VSQGSQEPRVRRGLKAPLGFLASWGRRERKAML
ATPLEEAEGNLALQGSLGPQGQREKQVSMARLAP
QGSQETRGSVEQLENRDQMAPRAPRENQGKERW
WITMETSMRLSRRSGRWP*WGLLVFLGKLAHLE
LQGFQARRGRLDCQALQDTMGKRDLAVNQETWAL
LVPKAPQERMDLQE*REKTGTQGAQERRGKKGR
QDKQAHRERKEKPGRRAIQEQRFLGCQGQRGLP
DLRGSKVFLDQRGKQD*MEQKERKASREKKETVV
PWDYPELQVWTAGLGHRVLQDQLEFQAQRDQRA
RGAAKETLG*QDQREQLGFLVYMDHPGTRETGG
RGGRKALEGLKGIRETKERLD*MPPAHWAKMAYQ
SKAAGTSDASNLGLACVCVCT*NIYFYTVFTF*
KCQKYDASYRLLKKKEKPAYFVQKISTSSLLFT
RCFV*AYVSNTFFVWS*CLHDICAHLLSIEA**I
IVSWCQRGPARTEVLASSCVNSHKCRGP*YLLS
*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEGRTEPGQHPRSTRAPRP
PWTKWTSGAPRTARAYGATWLTWASWTKGRPRD
PGLPRPEGRTGHARDARQAWSQGGARNCRGWDEG
*ARDPRNQG*EGG*RLPWASWPPGAEGRERRCW
QLHWRRQRGTWPSRAPWAPRAKGRSRCRWPGWP
PRAARRQGGAWSSWRTGTRWPQGLQGRTRERRDG
GLQWKHQ*GSPGDPDAGLDGASWSSWANWPTWS
SRDSRPEGGDWTARPSRTRWGKGTSR*TRRHGP
SWSPRPPRKGWTSRSEGRKRAPREPRREGGKRGD
RTSRLTGRERRSRGEGQSRSRGSWAARARGASR
TSGAPRCSWTKGGSRTRWSKRRERLPGRKRRPW
SPGTTRSFRFGRQAWATGYSRTNWSSRPSGTKGR
EGQQRRPWDDRTNGSSWASWFTWTTRGQGKPGG
EGEERL*RA*RG*GRPRSAWIRCPLPIGRRWLT
SPRLLEQVMPLTLDWPVCVFVHRIFIFIQFSLFE
NARSMMHLTDY*KRKKNLHILYRKYQPLPFCLQ
DVLYKPMSLIHFLFGRNVCMIFVHIY*VSKLNK
LLCPGAKGGQPELRCWLAHV*IHINVEVHDIC*A
RCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKGEQSQASIQGPPGPPGP
PGPSGPLGHPGLPGPMGPPGLPGPPGPKGDPGI
QGYHGRKGERGMPGMPGKHGAKGAPGIAVAGMK
GEPGIPGTKGEKGAEGSPGLPGLLGQKGEKGDAG
NSIGGGRGEPGPPGLPGPPGPKGEAGVDGQVGP
PGQPGDKGERGAAGEQGPDGPKGSKGEPGKGEM
VDYNGNINEALQEIRTLALMGPPGLPGQIGPPGA
PGIPGQKGEIGLPGPPGHDGEKGPRGKPGDMGP
PGPQGPPGKDGPPGVKGENGHPGSPGEKGEKGE
TGQAGSPGEKGEAGEKGNPGAEVPGLPGPEGPPG
PPGLQGVPGPKGEAGLDGAKGEKGFQGEKGDRG
PLGLPGASGLDGRPGPPGTPGPIGVPGPAGPKG
ERGSKGDPGMTGPTGAAGLPGLHGPPGDKGNRGE
RGKKGSRGPKGDKGDQGAPGLDAPCPLGEDGLP
VQGCWNK*CL*PWIGLCVCLYIEYLFLYSFHFL
KMPEV*CILQIIKKERKTCIFCTENINLFPFVYK
MFCISLCL*YIFCLVVMSA*YLCTFIKYRSLIN
YCVLVPKGASQN*GAG*LMCEFT*M*RSMIFAK
LGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080806

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGGCGAAC
AGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACC
TGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCAT
GGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGC
TTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCC
AGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGAT
GGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGA
TGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGA
TGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAAC
GGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAG
GAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGC
AAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACT
CCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTC
CTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCC
TGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGT
TTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCA
TATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGC
ATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATG
TGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGA
AACATGGCAATTC
  • refseq_COL13A1.F6 (forward primer)
  • refseq_COL13A1.R6 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGAN
RARPASKVHQGPQAPLDQVDLWGTQDCQGLWGH
LAYLGLLDQRETQGSRATTAGRENGACQGCQASM
EPRGRPELPWLG*RVSQGSQEPRVRRGLKAPLG
FLASWGRRERKAMLATPLEEAEGNLALQGSLGP
QGQREKQVSMARLAPQGSQETRGSVEQLENRDQM
APRAPRENQGKERWWITMETSMRLSRRSGRWP*
WGLLVFLGKLAHLELQGFQARRGRLDCQALQDT
MGKRDLAVNQETWALLVPKAPQERMDLQE*REKT
GTQGAQERRGKKGRQDKQAHRERKEKPGRRAIQ
EQRFLGCQGQRGLPDLRGSKVFLDQRGKQD*ME
QKERKASREKKETVVPWDYPELQVWTAGLGHRVL
QDQLEFQAQRDQRARGAAKETLG*QDQREQLGF
LVYMDHPGTRETGGRGGRKALEGLKGIRETKER
LD*MPPAHWAKMAYQSKAAGTSDASNLGLACVCV
CT*NIYFYTVFTF*KCQKYDASYRLLKKKEKPA
YFVQKISTSSLLFTRCFV*AYVSNTFFVWS*CL
HDICAHLLSIEA**IIVSWCQRGPARTEVLASSC
VNSHKCRGP*YLLS*VCLRVF*TLMDFHY*RK*
NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGRT
EPGQHPRSTRAPRPPWTKWTSGAPRTARAYGAT
WLTWASWTKGRPRDPGLPRPEGRTGHARDARQA
WSQGGARNCRGWDEG*ARDPRNQG*EGG*RLPWA
SWPPGAEGRERRCWQLHWRRQRGTWPSRAPWAP
RAKGRSRCRWPGWPPRAARRQGGAWSSWRTGTR
WPQGLQGRTRERRDGGLQWKHQ*GSPGDPDAGLD
GASWSSWANWPTWSSRDSRPEGGDWTARPSRTR
WGKGTSR*TRRHGPSWSPRPPRKGWTSRSEGRK
RAPREPRREGGKRGDRTSRLTGRERRSRGEGQSR
SRGSWAARARGASRTSGAPRCSWTKGGSRTRWS
KRRERLPGRKRRPWSPGTTRSFRFGRQAWATGY
SRTNWSSRPSGTKGREGQQRRPWDDRTNGSSWAS
WFTWTTRGQGKPGGEGEERL*RA*RG*GRPRSA
WIRCPLPIGRRWLTSPRLLEQVMPLTLDWPVCV
FVHRIFIFIQFSLFENARSMMHLTDY*KRKKNLH
ILYRKYQPLPFCLQDVLYKPMSLIHFLFGRNVC
MIFVHIY*VSKLNKLLCPGAKGGQPELRCWLAH
V*IHINVEVHDIC*ARCV*EYFKPLWIFIIKGNE
TWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHRGE
QSQASIQGPPGPPGPPGPSGPLGHPGLPGPMGPP
GLPGPPGPKGDPGIQGYHGRKGERGMPGMPGKH
GAKGAPGIAVAGMKGEPGIPGTKGEKGAEGSPG
LPGLLGQKGEKGDAGNSIGGGRGEPGPPGLPGPP
GPKGEAGVDGQVGPPGQPGDKGERGAAGEQGPD
GPKGSKGEPGKGEMVDYNGNINEALQEIRTLAL
MGPPGLPGQIGPPGAPGIPGQKGEIGLPGPPGHD
GEKGPRGKPGDMGPPGPQGPPGKDGPPGVKGEN
GHPGSPGEKGEKGETGQAGSPGEKGEAGEKGNP
GAEVPGLPGPEGPPGPPGLQGVPGPKGEAGLDGA
KGEKGFQGEKGDRGPLGLPGASGLDGRPGPPGT
PGPIGVPGPAGPKGERGSKGDPGMTGPTGAAGL
PGLHGPPGDKGNRGERGKKGSRGPKGDKGDQGAP
GLDAPCPLGEDGLPVQGCWNK*CL*PWIGLCVC
LYIEYLFLYSFHFLKMPEV*CILQIIKKERKTC
IFCTENINLFPFVYKMFCISLCL*YIFCLVVMSA
*YLCTFIKYRSLINYCVLVPKGASQN*GAG*LM
CEFT*M*RSMIFAKLGVSKSILNPYGFSLLKEM
KHGN

Sequence NM_080809

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA
AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA
CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA
TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA
GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC
CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG
CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA
GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA
CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC
CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGTTCCTGGGCTGCC
AGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAG
GGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAG
GAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGG
CTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGG
AACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTAT
GATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAG
CCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTG
CCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGA
GTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F6 (forward primer)
  • refseq_COL13A1.R6 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS*
KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD
CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA
CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR
RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA
LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE
QLENRDQMAPRAPRENQGKERWWITMETSMRLS
RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD
CQALQDTMGKRDLAVNQETWALLVPKAPQERMD
LQE*REKTGTQGAQERRGKKGRQDKQAHRFLGC
QGQRGLPDLRGSKVFLDQRGKQD*MEQKERKASR
EKKETVVPWDYPVLQDQLEFQAQRDQRARGAAK
ETLG*QDQREQLGFLVYMDHPGTRETGGRGGRK
ALEGLKGIRETKERLD*MPPAHWAKMAYQSKAAG
TSDASNLGLACVCVCT*NIYFYTVFTF*KCQKY
DASYRLLKKKEKPAYFVQKISTSSLLFTRCFV*
AYVSNTFFVWS*CLHDICAHLLSIEA**IIVSWC
QRGPARTEVLASSCVNSHKCRGP*YLLS*VCLR
VF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEAAASPQFSATGSTPGHK
KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR
TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH
ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E
GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW
PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS
SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP
GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW
TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT
SRSEGRKRAPREPRREGGKRGDRTSRLTGSWAA
RARGASRTSGAPRCSWTKGGSRTRWSKRRERLP
GRKRRPWSPGTTRYSRTNWSSRPSGTKGREGQQR
RPWDDRTNGSSWASWFTWTTRGQGKPGGEGEER
L*RA*RG*GRPRSAWIRCPLPIGRRWLTSPRLL
EQVMPLTLDWPVCVFVHRIFIFIQFSLFENARSM
MHLTDY*KRKKNLHILYRKYQPLPFCLQDVLYK
PMSLIHFLFGRNVCMIFVHIY*VSKLNKLLCPG
AKGGQPELRCWLAHV*IHINVEVHDIC*ARCV*E
YFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK
RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG
LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG
MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK
GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG
PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG
AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ
EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG
LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG
PPGVKGENGHPGSPGEKGEKGETGQAGSPVPGLP
GPEGPPGPPGLQGVPGPKGEAGLDGAKGEKGFQ
GEKGDRGPLGLPGTPGPIGVPGPAGPKGERGSK
GDPGMTGPTGAAGLPGLHGPPGDKGNRGERGKKG
SRGPKGDKGDQGAPGLDAPCPLGEDGLPVQGCW
NK*CL*PWIGLCVCLYIEYLFLYSFHFLKMPEV
*CILQIIKKERKTCIFCTENINLFPFVYKMFCIS
LCL*YIFCLVVMSA*YLCTFIKYRSLINYCVLV
PKGASQN*GAG*LMCEFT*M*RSMIFAKLGVSK
SILNPYGFSLLKEMKHGN

Sequence NM_080811

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCC
CCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATC
CAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGG
GTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGG
CAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCC
CCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGG
TGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGC
TCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCT
CCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGA
CAGGACAAGCAGGCTCACCGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGG
ACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTGGAGTTCCAGGC
CCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGG
ACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATT
GGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTA
TACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACC
TCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAA
GTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGG
TCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F6 (forward primer)
  • refseq_COL13A1.R6 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*RANRARPASKVHQGPQA
PLDQVDLWGTQDCQGLWGHLAYLGLLDQRETQGS
RATTAGRENGACQGCQASMEPRGRPELPWLG*R
VSQGSQEPRVRRGLKAPLGFLASWGRRERKAML
ATPLEEAEGNLALQGSLGPQGQREKQVSMARLAP
QGSQETRGSVEQLENRDQMAPRAPRENQGKERW
WITMETSMRLSRRSGRWP*WGLLVFLGKLAHLE
LQGFQARRGRLDCQALQDTMGKRDLAVNQETWAL
LVPKAPQERMDLQE*REKTGTQGAQERRGKKGR
QDKQAHRFLGCQGQRGLPDLRGSKVFLDQRGKQ
D*MEQKERKASREKKETVVPWDYPVLQDQLEFQA
QRDQRARGAAKETLG*QDQREQLGFLVYMDHPG
TRETGGRGGRKALEGLKGIRETKERLD*MPPAH
WAKMAYQSKAAGTSDASNLGLACVCVCT*NIYFY
TVFTF*KCQKYDASYRLLKKKEKPAYFVQKIST
SSLLFTRCFV*AYVSNTFFVWS*CLHDICAHLL
SIEA**IIVSWCQRGPARTEVLASSCVNSHKCRG
P*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEGRTEPGQHPRSTRAPRP
PWTKWTSGAPRTARAYGATWLTWASWTKGRPRD
PGLPRPEGRTGHARDARQAWSQGGARNCRGWDEG
*ARDPRNQG*EGG*RLPWASWPPGAEGRERRCW
QLHWRRQRGTWPSRAPWAPRAKGRSRCRWPGWP
PRAARRQGGAWSSWRTGTRWPQGLQGRTRERRDG
GLQWKHQ*GSPGDPDAGLDGASWSSWANWPTWS
SRDSRPEGGDWTARPSRTRWGKGTSR*TRRHGP
SWSPRPPRKGWTSRSEGRKRAPREPRREGGKRGD
RTSRLTGSWAARARGASRTSGAPRCSWTKGGSR
TRWSKRRERLPGRKRRPWSPGTTRYSRTNWSSR
PSGTKGREGQQRRPWDDRTNGSSWASWFTWTTRG
QGKPGGEGEERL*RA*RG*GRPRSAWIRCPLPI
GRRWLTSPRLLEQVMPLTLDWPVCVFVHRIFIF
IQFSLFENARSMMHLTDY*KRKKNLHILYRKYQP
LPFCLQDVLYKPMSLIHFLFGRNVCMIFVHIY*
VSKLNKLLCPGAKGGQPELRCWLAHV*IHINVE
VHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKGEQSQASIQGPPGPPGP
PGPSGPLGHPGLPGPMGPPGLPGPPGPKGDPGI
QGYHGRKGERGMPGMPGKHGAKGAPGIAVAGMK
GEPGIPGTKGEKGAEGSPGLPGLLGQKGEKGDAG
NSIGGGRGEPGPPGLPGPPGPKGEAGVDGQVGP
PGQPGDKGERGAAGEQGPDGPKGSKGEPGKGEM
VDYNGNINEALQEIRTLALMGPPGLPGQIGPPGA
PGIPGQKGEIGLPGPPGHDGEKGPRGKPGDMGP
PGPQGPPGKDGPPGVKGENGHPGSPGEKGEKGE
TGQAGSPVPGLPGPEGPPGPPGLQGVPGPKGEAG
LDGAKGEKGFQGEKGDRGPLGLPGTPGPIGVPG
PAGPKGERGSKGDPGMTGPTGAAGLPGLHGPPG
DKGNRGERGKKGSRGPKGDKGDQGAPGLDAPCPL
GEDGLPVQGCWNK*CL*PWIGLCVCLYIEYLFL
YSFHFLKMPEV*CILQIIKKERKTCIFCTENIN
LFPFVYKMFCISLCL*YIFCLVVMSA*YLCTFIK
YRSLINYCVLVPKGASQN*GAG*LMCEFT*M*R
SMIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080800

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGCTGCTGC
CTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCC
CCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGAC
CCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTG
GGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGG
CGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAG
GTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAG
GAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCC
ACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGAC
ATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAA
AAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGG
GCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGA
GACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGAC
CAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAA
CCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGAT
GGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTC
ACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTT
TGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGC
TTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATAT
TTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F6 (forward primer)
  • refseq_COL13A1.R6 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGCC
LSSIQCDWLHPRS*KGGRSRANRARPASKVHQG
PQAPLDQVDLWGTQDCQGLWGHLAYLGLLDQRET
QGSRATTAGRENGACQGCQASMEPRGRPELPWL
G*RVSQGSQEPRVRRGLKAPLGFLASWGRRERK
AMLATPLEEAEGNLALQGSLGPQGQREKQVSMAR
LAPQGSQETRGSVEQLENRDQMAPRAPRENQGK
ERWWITMETSMRLSRRSGRWP*WGLLVFLGKLA
HLELQGFQARRGRLDCQALQDTMGKRDLAVNQET
WALLVPKAPQERMDLQE*REKTGTQGAQERRGK
KGRQDKQAHRERKEKPGRRAIQEQRFLGCQGQR
GLPDLRGSKVFLDQRGKQD*MEQKERKASREKKE
TVVPWDYPELQVWTAGLGHRVLQDQLEFQAQRD
QRARGAAKETLG*QDQREQLGFLVYMDHPGTRE
TGGRGGRKALEGLKGIRETKERLD*MPPAHWAKM
AYQSKAAGTSDASNLGLACVCVCT*NIYFYTVF
TF*KCQKYDASYRLLKKKEKPAYFVQKISTSSL
LFTRCFV*AYVSNTFFVWS*CLHDICAHLLSIEA
**IIVSWCQRGPARTEVLASSCVNSHKCRGP*Y
LLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPAAA
SPQFSATGSTPGHKKADVPGRTEPGQHPRSTRA
PRPPWTKWTSGAPRTARAYGATWLTWASWTKGR
PRDPGLPRPEGRTGHARDARQAWSQGGARNCRGW
DEG*ARDPRNQG*EGG*RLPWASWPPGAEGRER
RCWQLHWRRQRGTWPSRAPWAPRAKGRSRCRWP
GWPPRAARRQGGAWSSWRTGTRWPQGLQGRTRER
RDGGLQWKHQ*GSPGDPDAGLDGASWSSWANWP
TWSSRDSRPEGGDWTARPSRTRWGKGTSR*TRR
HGPSWSPRPPRKGWTSRSEGRKRAPREPRREGGK
RGDRTSRLTGRERRSRGEGQSRSRGSWAARARG
ASRTSGAPRCSWTKGGSRTRWSKRRERLPGRKR
RPWSPGTTRSFRFGRQAWATGYSRTNWSSRPSGT
KGREGQQRRPWDDRTNGSSWASWFTWTTRGQGK
PGGEGEERL*RA*RG*GRPRSAWIRCPLPIGRR
WLTSPRLLEQVMPLTLDWPVCVFVHRIFIFIQFS
LFENARSMMHLTDY*KRKKNLHILYRKYQPLPF
CLQDVLYKPMSLIHFLFGRNVCMIFVHIY*VSK
LNKLLCPGAKGGQPELRCWLAHV*IHINVEVHDI
C*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHRLL
PLLNSVRLAPPPVIKRRTFQGEQSQASIQGPPGP
PGPPGPSGPLGHPGLPGPMGPPGLPGPPGPKGD
PGIQGYHGRKGERGMPGMPGKHGAKGAPGIAVA
GMKGEPGIPGTKGEKGAEGSPGLPGLLGQKGEKG
DAGNSIGGGRGEPGPPGLPGPPGPKGEAGVDGQ
VGPPGQPGDKGERGAAGEQGPDGPKGSKGEPGK
GEMVDYNGNINEALQEIRTLALMGPPGLPGQIGP
PGAPGIPGQKGEIGLPGPPGHDGEKGPRGKPGD
MGPPGPQGPPGKDGPPGVKGENGHPGSPGEKGE
KGETGQAGSPGEKGEAGEKGNPGAEVPGLPGPEG
PPGPPGLQGVPGPKGEAGLDGAKGEKGFQGEKG
DRGPLGLPGASGLDGRPGPPGTPGPIGVPGPAG
PKGERGSKGDPGMTGPTGAAGLPGLHGPPGDKGN
RGERGKKGSRGPKGDKGDQGAPGLDAPCPLGED
GLPVQGCWNK*CL*PWIGLCVCLYIEYLFLYSF
HFLKMPEV*CILQIIKKERKTCIFCTENINLFPF
VYKMFCISLCL*YIFCLVVMSA*YLCTFIKYRS
LINYCVLVPKGASQN*GAG*LMCEFT*M*RSMI
FAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080808

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA
AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA
CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA
TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA
GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC
CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG
CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA
GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA
CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC
CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGA
AGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTC
CAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCA
AAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAA
AGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGC
TGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAG
TATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTAT
AAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTG
GTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTA
AGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F6 (forward primer)
  • refseq_COL13A1.R6 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS*
KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD
CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA
CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR
RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA
LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE
QLENRDQMAPRAPRENQGKERWWITMETSMRLS
RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD
CQALQDTMGKRDLAVNQETWALLVPKAPQERMD
LQE*REKTGTQGAQERRGKKGRQDKQAHRERKE
KPGRRAIQEQRGSKVFLDQRGKQD*MEQKERKAS
REKKETVVPWDYPVLQDQLEFQAQRDQRARGAA
KETLG*QDQREQLGFLVYMDHPGTRETGGRGGR
KALEGLKGIRETKERLD*MPPAHWAKMAYQSKAA
GTSDASNLGLACVCVCT*NIYFYTVFTF*KCQK
YDASYRLLKKKEKPAYFVQKISTSSLLFTRCFV
*AYVSNTFFVWS*CLHDICAHLLSIEA**IIVSW
CQRGPARTEVLASSCVNSHKCRGP*YLLS*VCL
RVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEAAASPQFSATGSTPGHK
KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR
TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH
ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E
GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW
PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS
SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP
GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW
TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT
SRSEGRKRAPREPRREGGKRGDRTSRLTGRERR
SRGEGQSRSRGAPRCSWTKGGSRTRWSKRRERL
PGRKRRPWSPGTTRYSRTNWSSRPSGTKGREGQQ
RRPWDDRTNGSSWASWFTWTTRGQGKPGGEGEE
RL*RA*RG*GRPRSAWIRCPLPIGRRWLTSPRL
LEQVMPLTLDWPVCVFVHRIFIFIQFSLFENARS
MMHLTDY*KRKKNLHILYRKYQPLPFCLQDVLY
KPMSLIHFLFGRNVCMIFVHIY*VSKLNKLLCP
GAKGGQPELRCWLAHV*IHINVEVHDIC*ARCV*
EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK
RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG
LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG
MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK
GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG
PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG
AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ
EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG
LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG
PPGVKGENGHPGSPGEKGEKGETGQAGSPGEKGE
AGEKGNPGAEGLQGVPGPKGEAGLDGAKGEKGF
QGEKGDRGPLGLPGTPGPIGVPGPAGPKGERGS
KGDPGMTGPTGAAGLPGLHGPPGDKGNRGERGKK
GSRGPKGDKGDQGAPGLDAPCPLGEDGLPVQGC
WNK*CL*PWIGLCVCLYIEYLFLYSFHFLKMPE
V*CILQIIKKERKTCIFCTENINLFPFVYKMFCI
SLCL*YIFCLVVMSA*YLCTFIKYRSLINYCVL
VPKGASQN*GAG*LMCEFT*M*RSMIFAKLGVS
KSILNPYGFSLLKEMKHGN

Sequence NM_005203

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA
AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA
CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA
TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA
GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC
CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG
CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA
GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA
CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC
CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGA
AGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAG
GGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACG
GCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGG
ACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGG
GATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAAC
CTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTAT
TAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATT
TTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGA
ACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGG
ATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F6 (forward primer)
  • refseq_COL13A1.R6 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS*
KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD
CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA
CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR
RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA
LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE
QLENRDQMAPRAPRENQGKERWWITMETSMRLS
RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD
CQALQDTMGKRDLAVNQETWALLVPKAPQERMD
LQE*REKTGTQGAQERRGKKGRQDKQAHRERKE
KPGRRAIQEQRFLGCQGQRGLPDLRGSKVFLDQR
GKQD*MEQKERKASREKKETVVPWDYPELQVWT
AGLGHRVLQDQLEFQAQRDQRARGAAKETLG*Q
DQREQLGFLVYMDHPGTRETGGRGGRKALEGLKG
IRETKERLD*MPPAHWAKMAYQSKAAGTSDASN
LGLACVCVCT*NIYFYTVFTF*KCQKYDASYRL
LKKKEKPAYFVQKISTSSLLFTRCFV*AYVSNTF
FVWS*CLHDICAHLLSIEA**IIVSWCQRGPAR
TEVLASSCVNSHKCRGP*YLLS*VCLRVF*TLM
DFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEAAASPQFSATGSTPGHK
KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR
TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH
ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E
GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW
PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS
SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP
GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW
TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT
SRSEGRKRAPREPRREGGKRGDRTSRLTGRERR
SRGEGQSRSRGSWAARARGASRTSGAPRCSWTK
GGSRTRWSKRRERLPGRKRRPWSPGTTRSFRFGR
QAWATGYSRTNWSSRPSGTKGREGQQRRPWDDR
TNGSSWASWFTWTTRGQGKPGGEGEERL*RA*R
G*GRPRSAWIRCPLPIGRRWLTSPRLLEQVMPLT
LDWPVCVFVHRIFIFIQFSLFENARSMMHLTDY
*KRKKNLHILYRKYQPLPFCLQDVLYKPMSLIH
FLFGRNVCMIFVHIY*VSKLNKLLCPGAKGGQPE
LRCWLAHV*IHINVEVHDIC*ARCV*EYFKPLW
IFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK
RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG
LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG
MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK
GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG
PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG
AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ
EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG
LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG
PPGVKGENGHPGSPGEKGEKGETGQAGSPGEKGE
AGEKGNPGAEVPGLPGPEGPPGPPGLQGVPGPK
GEAGLDGAKGEKGFQGEKGDRGPLGLPGASGLD
GRPGPPGTPGPIGVPGPAGPKGERGSKGDPGMTG
PTGAAGLPGLHGPPGDKGNRGERGKKGSRGPKG
DKGDQGAPGLDAPCPLGEDGLPVQGCWNK*CL*
PWIGLCVCLYIEYLFLYSFHFLKMPEV*CILQII
KKERKTCIFCTENINLFPFVYKMFCISLCL*YI
FCLVVMSA*YLCTFIKYRSLINYCVLVPKGASQ
N*GAG*LMCEFT*M*RSMIFAKLGVSKSILNPYG
FSLLKEMKHGN

Sequence NM_080802

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA
AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA
CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA
TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA
GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC
CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG
CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA
GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA
CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC
CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGTTCCTGGGCTGCC
AGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAG
GGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAG
GCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGG
GGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCA
TTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTT
TATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAA
CCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATT
AAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGA
GGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F6 (forward primer)
  • refseq_COL13A1.R6 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS*
KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD
CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA
CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR
RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA
LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE
QLENRDQMAPRAPRENQGKERWWITMETSMRLS
RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD
CQALQDTMGKRDLAVNQETWALLVPKAPQERMD
LQE*REKTGTQGAQERRGKKGRQDKQAHRFLGC
QGQRGLPDLRGSKVFLDQRGKQD*MEQKERKASR
EKKETVVPWDYPELQVWTAGLGHRVLQDQLEFQ
AQRDQRARGAAKETLG*QDQREQLGFLVYMDHP
GTRETGGRGGRKALEGLKGIRETKERLD*MPPAH
WAKMAYQSKAAGTSDASNLGLACVCVCT*NIYF
YTVFTF*KCQKYDASYRLLKKKEKPAYFVQKIS
TSSLLFTRCFV*AYVSNTFFVWS*CLHDICAHLL
SIEA**IIVSWCQRGPARTEVLASSCVNSHKCR
GP*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEAAASPQFSATGSTPGHK
KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR
TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH
ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E
GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW
PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS
SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP
GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW
TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT
SRSEGRKRAPREPRREGGKRGDRTSRLTGSWAA
RARGASRTSGAPRCSWTKGGSRTRWSKRRERLP
GRKRRPWSPGTTRSFRFGRQAWATGYSRTNWSSR
PSGTKGREGQQRRPWDDRTNGSSWASWFTWTTR
GQGKPGGEGEERL*RA*RG*GRPRSAWIRCPLP
IGRRWLTSPRLLEQVMPLTLDWPVCVFVHRIFIF
IQFSLFENARSMMHLTDY*KRKKNLHILYRKYQ
PLPFCLQDVLYKPMSLIHFLFGRNVCMIFVHIY
*VSKLNKLLCPGAKGGQPELRCWLAHV*IHINVE
VHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK
RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG
LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG
MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK
GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG
PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG
AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ
EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG
LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG
PPGVKGENGHPGSPGEKGEKGETGQAGSPVPGLP
GPEGPPGPPGLQGVPGPKGEAGLDGAKGEKGFQ
GEKGDRGPLGLPGASGLDGRPGPPGTPGPIGVP
GPAGPKGERGSKGDPGMTGPTGAAGLPGLHGPPG
DKGNRGERGKKGSRGPKGDKGDQGAPGLDAPCP
LGEDGLPVQGCWNK*CL*PWIGLCVCLYIEYLF
LYSFHFLKMPEV*CILQIIKKERKTCIFCTENIN
LFPFVYKMFCISLCL*YIFCLVVMSA*YLCTFI
KYRSLINYCVLVPKGASQN*GAG*LMCEFT*M*
RSMIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080804

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA
AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA
CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA
TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA
GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC
CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG
CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA
GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA
CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC
CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGA
AGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAG
GGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTG
GAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGG
ACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCC
CCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAAT
ATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGA
AAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGC
ACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACAT
AAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F6 (forward primer)
  • refseq_COL13A1.R6 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS*
KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD
CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA
CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR
RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA
LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE
QLENRDQMAPRAPRENQGKERWWITMETSMRLS
RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD
CQALQDTMGKRDLAVNQETWALLVPKAPQERMD
LQE*REKTGTQGAQERRGKKGRQDKQAHRERKE
KPGRRAIQEQRFLGCQGQRGLPDLRGSKVFLDQR
GKQD*MEQKERKASREKKETVVPWDYPVLQDQL
EFQAQRDQRARGAAKETLG*QDQREQLGFLVYM
DHPGTRETGGRGGRKALEGLKGIRETKERLD*MP
PAHWAKMAYQSKAAGTSDASNLGLACVCVCT*N
IYFYTVFTF*KCQKYDASYRLLKKKEKPAYFVQ
KISTSSLLFTRCFV*AYVSNTFFVWS*CLHDICA
HLLSIEA**IIVSWCQRGPARTEVLASSCVNSH
KCRGP*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEAAASPQFSATGSTPGHK
KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR
TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH
ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E
GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW
PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS
SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP
GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW
TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT
SRSEGRKRAPREPRREGGKRGDRTSRLTGRERR
SRGEGQSRSRGSWAARARGASRTSGAPRCSWTK
GGSRTRWSKRRERLPGRKRRPWSPGTTRYSRTNW
SSRPSGTKGREGQQRRPWDDRTNGSSWASWFTW
TTRGQGKPGGEGEERL*RA*RG*GRPRSAWIRC
PLPIGRRWLTSPRLLEQVMPLTLDWPVCVFVHRI
FIFIQFSLFENARSMMHLTDY*KRKKNLHILYR
KYQPLPFCLQDVLYKPMSLIHFLFGRNVCMIFV
HIY*VSKLNKLLCPGAKGGQPELRCWLAHV*IHI
NVEVHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK
RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG
LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG
MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK
GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG
PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG
AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ
EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG
LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG
PPGVKGENGHPGSPGEKGEKGETGQAGSPGEKGE
AGEKGNPGAEVPGLPGPEGPPGPPGLQGVPGPK
GEAGLDGAKGEKGFQGEKGDRGPLGLPGTPGPI
GVPGPAGPKGERGSKGDPGMTGPTGAAGLPGLHG
PPGDKGNRGERGKKGSRGPKGDKGDQGAPGLDA
PCPLGEDGLPVQGCWNK*CL*PWIGLCVCLYIE
YLFLYSFHFLKMPEV*CILQIIKKERKTCIFCTE
NINLFPFVYKMFCISLCL*YIFCLVVMSA*YLC
TFIKYRSLINYCVLVPKGASQN*GAG*LMCEFT
*M*RSMIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080803

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA
AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA
CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA
TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA
GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC
CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG
CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA
GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA
CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC
CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGA
AGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTC
CAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTC
CAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACC
CGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGC
CCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTAT
TTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATAT
CAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTT
ATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGT
AGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F6 (forward primer)
  • refseq_COL13A1.R6 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS*
KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD
CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA
CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR
RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA
LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE
QLENRDQMAPRAPRENQGKERWWITMETSMRLS
RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD
CQALQDTMGKRDLAVNQETWALLVPKAPQERMD
LQE*REKTGTQGAQERRGKKGRQDKQAHRERKE
KPGRRAIQEQRGSKVFLDQRGKQD*MEQKERKAS
REKKETVVPWDYPELQVWTAGLGHRVLQDQLEF
QAQRDQRARGAAKETLG*QDQREQLGFLVYMDH
PGTRETGGRGGRKALEGLKGIRETKERLD*MPPA
HWAKMAYQSKAAGTSDASNLGLACVCVCT*NIY
FYTVFTF*KCQKYDASYRLLKKKEKPAYFVQKI
STSSLLFTRCFV*AYVSNTFFVWS*CLHDICAHL
LSIEA**IIVSWCQRGPARTEVLASSCVNSHKC
RGP*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEAAASPQFSATGSTPGHK
KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR
TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH
ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E
GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW
PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS
SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP
GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW
TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT
SRSEGRKRAPREPRREGGKRGDRTSRLTGRERR
SRGEGQSRSRGAPRCSWTKGGSRTRWSKRRERL
PGRKRRPWSPGTTRSFRFGRQAWATGYSRTNWSS
RPSGTKGREGQQRRPWDDRTNGSSWASWFTWTT
RGQGKPGGEGEERL*RA*RG*GRPRSAWIRCPL
PIGRRWLTSPRLLEQVMPLTLDWPVCVFVHRIFI
FIQFSLFENARSMMHLTDY*KRKKNLHILYRKY
QPLPFCLQDVLYKPMSLIHFLFGRNVCMIFVHI
Y*VSKLNKLLCPGAKGGQPELRCWLAHV*IHINV
EVHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK
RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG
LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG
MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK
GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG
PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG
AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ
EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG
LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG
PPGVKGENGHPGSPGEKGEKGETGQAGSPGEKGE
AGEKGNPGAEGLQGVPGPKGEAGLDGAKGEKGF
QGEKGDRGPLGLPGASGLDGRPGPPGTPGPIGV
PGPAGPKGERGSKGDPGMTGPTGAAGLPGLHGPP
GDKGNRGERGKKGSRGPKGDKGDQGAPGLDAPC
PLGEDGLPVQGCWNK*CL*PWIGLCVCLYIEYL
FLYSFHFLKMPEV*CILQIIKKERKTCIFCTENI
NLFPFVYKMFCISLCL*YIFCLVVMSA*YLCTF
IKYRSLINYCVLVPKGASQN*GAG*LMCEFT*M
*RSMIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080807

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGCTGCTGC
CTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCC
CCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGAC
CCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTG
GGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGG
CGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAG
GTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAG
GAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCC
ACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGAC
ATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAA
AAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGG
GCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGA
GACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGA
TGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCC
TAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGC
CTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTAC
AGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTA
ATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCC
AGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACC
CTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F6 (forward primer)
  • refseq_COL13A1.R6 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGCC
LSSIQCDWLHPRS*KGGRSRANRARPASKVHQG
PQAPLDQVDLWGTQDCQGLWGHLAYLGLLDQRET
QGSRATTAGRENGACQGCQASMEPRGRPELPWL
G*RVSQGSQEPRVRRGLKAPLGFLASWGRRERK
AMLATPLEEAEGNLALQGSLGPQGQREKQVSMAR
LAPQGSQETRGSVEQLENRDQMAPRAPRENQGK
ERWWITMETSMRLSRRSGRWP*WGLLVFLGKLA
HLELQGFQARRGRLDCQALQDTMGKRDLAVNQET
WALLVPKAPQERMDLQE*REKTGTQGAQERRGK
KGRQDKQAHRERKEKPGRRAIQEQRFLGCQGQR
GLPDLRGSKVFLDQRGKQD*MEQKERKASREKKE
TVVPWDYPVLQDQLEFQAQRDQRARGAAKETLG
*QDQREQLGFLVYMDHPGTRETGGRGGRKALEG
LKGIRETKERLD*MPPAHWAKMAYQSKAAGTSDA
SNLGLACVCVCT*NIYFYTVFTF*KCQKYDASY
RLLKKKEKPAYFVQKISTSSLLFTRCFV*AYVS
NTFFVWS*CLHDICAHLLSIEA**IIVSWCQRGP
ARTEVLASSCVNSHKCRGP*YLLS*VCLRVF*T
LMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPAAA
SPQFSATGSTPGHKKADVPGRTEPGQHPRSTRA
PRPPWTKWTSGAPRTARAYGATWLTWASWTKGR
PRDPGLPRPEGRTGHARDARQAWSQGGARNCRGW
DEG*ARDPRNQG*EGG*RLPWASWPPGAEGRER
RCWQLHWRRQRGTWPSRAPWAPRAKGRSRCRWP
GWPPRAARRQGGAWSSWRTGTRWPQGLQGRTRER
RDGGLQWKHQ*GSPGDPDAGLDGASWSSWANWP
TWSSRDSRPEGGDWTARPSRTRWGKGTSR*TRR
HGPSWSPRPPRKGWTSRSEGRKRAPREPRREGGK
RGDRTSRLTGRERRSRGEGQSRSRGSWAARARG
ASRTSGAPRCSWTKGGSRTRWSKRRERLPGRKR
RPWSPGTTRYSRTNWSSRPSGTKGREGQQRRPWD
DRTNGSSWASWFTWTTRGQGKPGGEGEERL*RA
*RG*GRPRSAWIRCPLPIGRRWLTSPRLLEQVM
PLTLDWPVCVFVHRIFIFIQFSLFENARSMMHLT
DY*KRKKNLHILYRKYQPLPFCLQDVLYKPMSL
IHFLFGRNVCMIFVHIY*VSKLNKLLCPGAKGG
QPELRCWLAHV*IHINVEVHDIC*ARCV*EYFKP
LWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHRLL
PLLNSVRLAPPPVIKRRTFQGEQSQASIQGPPGP
PGPPGPSGPLGHPGLPGPMGPPGLPGPPGPKGD
PGIQGYHGRKGERGMPGMPGKHGAKGAPGIAVA
GMKGEPGIPGTKGEKGAEGSPGLPGLLGQKGEKG
DAGNSIGGGRGEPGPPGLPGPPGPKGEAGVDGQ
VGPPGQPGDKGERGAAGEQGPDGPKGSKGEPGK
GEMVDYNGNINEALQEIRTLALMGPPGLPGQIGP
PGAPGIPGQKGEIGLPGPPGHDGEKGPRGKPGD
MGPPGPQGPPGKDGPPGVKGENGHPGSPGEKGE
KGETGQAGSPGEKGEAGEKGNPGAEVPGLPGPEG
PPGPPGLQGVPGPKGEAGLDGAKGEKGFQGEKG
DRGPLGLPGTPGPIGVPGPAGPKGERGSKGDPG
MTGPTGAAGLPGLHGPPGDKGNRGERGKKGSRGP
KGDKGDQGAPGLDAPCPLGEDGLPVQGCWNK*C
L*PWIGLCVCLYIEYLFLYSFHFLKMPEV*CIL
QIIKKERKTCIFCTENINLFPFVYKMFCISLCL*
YIFCLVVMSA*YLCTFIKYRSLINYCVLVPKGA
SQN*GAG*LMCEFT*M*RSMIFAKLGVSKSILN
PYGFSLLKEMKHGN

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