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Sequences

Sequence NM_171827

CGAAAAGGAGGGTGACTCTCCTCGGCGGGGGCTTCGGGTGACATCACATCCTCCAAATGCGAAATCAGGCTCCGGGCCGGCCGAAGGGCGCAACTTTCCC
CCCTCGGCGCCCCACCGGCTCCCGCGCGCCTCCCCTCGCGCCCGAGCTTCGAGCCAAGCAGCGTCCTGGGGAGCGCGTCATGGCCTTACCAGTGACCGCC
TTGCTCCTGCCGCTGGCCTTGCTGCTCCACGCCGCCAGGCCGAGCCAGTTCCGGGTGTCGCCGCTGGATCGGACCTGGAACCTGGGCGAGACAGTGGAGC
TGAAGTGCCAGGTGCTGCTGTCCAACCCGACGTCGGGCTGCTCGTGGCTCTTCCAGCCGCGCGGCGCCGCCGCCAGTCCCACCTTCCTCCTATACCTCTC
CCAAAACAAGCCCAAGGCGGCCGAGGGGCTGGACACCCAGCGGTTCTCGGGCAAGAGGTTGGGGGACACCTTCGTCCTCACCCTGAGCGACTTCCGCCGA
GAGAACGAGGGCTACTATTTCTGCTCGGCCCTGAGCAACTCCATCATGTACTTCAGCCACTTCGTGCCGGTCTTCCTGCCAGCGAAGCCCACCACGACGC
CAGCGCCGCGACCACCAACACCGGCGCCCACCATCGCGTCGCAGCCCCTGTCCCTGCGCCCAGAGGCGTGCCGGCCAGCGGCGGGGGGCGCAGGGAACCG
AAGACGTGTTTGCAAATGTCCCCGGCCTGTGGTCAAATCGGGAGACAAGCCCAGCCTTTCGGCGAGATACGTCTAACCCTGTGCAACAGCCACTACATTA
CTTCAAACTGAGATCCTTCCTTTTGAGGGAGCAAGTCCTTCCCTTTCATTTTTTCCAGTCTTCCTCCCTGTGTATTCATTCTCATGATTATTATTTTAGT
GGGGGCGGGGTGGGAAAGATTACTTTTTCTTTATGTGTTTGACGGGAAACAAAACTAGGTAAAATCTACAGTACACCACAAGGGTCACAATACTGTTGTG
CGCACATCGCGGTAGGGCGTGGAAAGGGGCAGGCCAGAGCTACCCGCAGAGTTCTCAGAATCATGCTGAGAGAGCTGGAGGCACCCATGCCATCTCAACC
TCTTCCCCGCCCGTTTTACAAAGGGGGAGGCTAAAGCCCAGAGACAGCTTGATCAAAGGCACACAGCAAGTCAGGGTTGGAGCAGTAGCTGGAGGGACCT
TGTCTCCCAGCTCAGGGCTCTTTCCTCCACACCATTCAGGTCTTTCTTTCCGAGGCCCCTGTCTCAGGGTGAGGTGCTTGAGTCTCCAACGGCAAGGGAA
CAAGTACTTCTTGATACCTGGGATACTGTGCCCAGAGCCTCGAGGAGGTAATGAATTAAAGAAGAGAACTGCCTTTGGCAGAGTTCTATAATGTAAACAA
TATCAGACTTTTTTTTTTTATAATCAAGCCTAAAATTGTATAGACCTAAAATAAAATGAAGTGGTGAGCTTAACCCTGGAAAATGAATCCCTCTATCTCT
AAAGAAAATCTCTGTGAAACCCCTATGTGGAGGCGGAATTGCTCTCCCAGCCCTTGCATTGCAGAGGGGCCCATGAAAGAGGACAGGCTACCCCTTTACA
AATAGAATTTGAGCATCAGTGAGGTTAAACTAAGGCCCTCTTGAATCTCTGAATTTGAGATACAAACATGTTCCTGGGATCACTGATGACTTTTTATACT
TTGTAAAGACAATTGTTGGAGAGCCCCTCACACAGCCCTGGCCTCTGCTCAACTAGCAGATACAGGGATGAGGCAGACCTGACTCTCTTAAGGAGGCTGA
GAGCCCAAACTGCTGTCCCAAACATGCACTTCCTTGCTTAAGGTATGGTACAAGCAATGCCTGCCCATTGGAGAGAAAAAACTTAAGTAGATAAGGAAAT
AAGAACCACTCATAATTCTTCACCTTAGGAATAATCTCCTGTTAATATGGTGTACATTCTTCCTGATTATTTTCTACACATACATGTAAAATATGTCTTT
CTTTTTTAAATAGGGTTGTACTATGCTGTTATGAGTGGCTTTAATGAATAAACATTTGTAGCATCCTCTTTAATGGGTAAACAGCATCCGA
  • refseq_CD8A.F2 (forward primer)
  • refseq_CD8A.R2 (reverse primer)
  • Single exon skipping

Frame 0

RKGG*LSSAGASGDITSSKCEIRLRAGRRAQLS
PLGAPPAPARLPSRPSFEPSSVLGSASWPYQ*PP
CSCRWPCCSTPPGRASSGCRRWIGPGTWARQWS
*SARCCCPTRRRAARGSSSRAAPPPVPPSSYTS
PKTSPRRPRGWTPSGSRARGWGTPSSSP*ATSAE
RTRATISARP*ATPSCTSATSCRSSCQRSPPRR
QRRDHQHRRPPSRRSPCPCAQRRAGQRRGAQGT
EDVFANVPGLWSNRETSPAFRRDTSNPVQQPLHY
FKLRSFLLREQVLPFHFFQSSSLCIHSHDYYFS
GGGVGKITFSLCV*RETKLGKIYSTPQGSQYCC
AHIAVGRGKGQARATRRVLRIMLRELEAPMPSQP
LPRPFYKGGG*SPETA*SKAHSKSGLEQ*LEGP
CLPAQGSFLHTIQVFLSEAPVSG*GA*VSNGKG
TSTS*YLGYCAQSLEEVMN*RRELPLAEFYNVNN
IRLFFFIIKPKIV*T*NKMKW*A*PWKMNPSIS
KENLCETPMWRRNCSPSPCIAEGPMKEDRLPLY
K*NLSISEVKLRPS*ISEFEIQTCSWDH**LFIL
CKDNCWRAPHTALASAQLADTGMRQT*LS*GG*
EPKLLSQTCTSLLKVWYKQCLPIGEKKLK*IRK
*EPLIILHLRNNLLLIWCTFFLIIFYTYM*NMSF
FFK*GCTMLL*VALMNKHL*HPL*WVNSIR

Frame 1

EKEGDSPRRGLRVTSHPPNAKSGSGPAEGRNFP
PSAPHRLPRASPRARASSQAASWGARHGLTSDR
LAPAAGLAAPRRQAEPVPGVAAGSDLEPGRDSGA
EVPGAAVQPDVGLLVALPAARRRRQSHLPPIPL
PKQAQGGRGAGHPAVLGQEVGGHLRPHPERLPP
RERGLLFLLGPEQLHHVLQPLRAGLPASEAHHDA
SAATTNTGAHHRVAAPVPAPRGVPASGGGRREP
KTCLQMSPACGQIGRQAQPFGEIRLTLCNSHYI
TSN*DPSF*GSKSFPFIFSSLPPCVFILMIIILV
GAGWERLLFLYVFDGKQN*VKSTVHHKGHNTVV
RTSR*GVERGRPELPAEFSESC*ESWRHPCHLN
LFPARFTKGEAKAQRQLDQRHTASQGWSSSWRDL
VSQLRALSSTPFRSFFPRPLSQGEVLESPTARE
QVLLDTWDTVPRASRR**IKEENCLWQSSIM*T
ISDFFFL*SSLKLYRPKIK*SGELNPGK*IPLSL
KKISVKPLCGGGIALPALALQRGP*KRTGYPFT
NRI*ASVRLN*GPLESLNLRYKHVPGITDDFLY
FVKTIVGEPLTQPWPLLN*QIQG*GRPDSLKEAE
SPNCCPKHALPCLRYGTSNACPLERKNLSR*GN
KNHS*FFTLGIISC*YGVHSS*LFSTHTCKICL
SFLNRVVLCCYEWL**INICSILFNG*TAS

Frame 2

KRRVTLLGGGFG*HHILQMRNQAPGRPKGATFP
PRRPTGSRAPPLAPELRAKQRPGERVMALPVTA
LLLPLALLLHAARPSQFRVSPLDRTWNLGETVE
LKCQVLLSNPTSGCSWLFQPRGAAASPTFLLYLS
QNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRR
ENEGYYFCSALSNSIMYFSHFVPVFLPAKPTTT
PAPRPPTPAPTIASQPLSLRPEACRPAAGGAGNR
RRVCKCPRPVVKSGDKPSLSARYV*PCATATTL
LQTEILPFEGASPSLSFFPVFLPVYSFS*LLF*
WGRGGKDYFFFMCLTGNKTR*NLQYTTRVTILLC
AHRGRAWKGAGQSYPQSSQNHAERAGGTHAIST
SSPPVLQRGRLKPRDSLIKGTQQVRVGAVAGGT
LSPSSGLFPPHHSGLSFRGPCLRVRCLSLQRQGN
KYFLIPGILCPEPRGGNELKKRTAFGRVL*CKQ
YQTFFFYNQA*NCIDLK*NEVVSLTLENESLYL
*RKSL*NPYVEAELLSQPLHCRGAHERGQATPLQ
IEFEHQ*G*TKALLNL*I*DTNMFLGSLMTFYT
L*RQLLESPSHSPGLCSTSRYRDEADLTLLRRL
RAQTAVPNMHFLA*GMVQAMPAHWREKT*VDKEI
RTTHNSSP*E*SPVNMVYILPDYFLHIHVKYVF
LF*IGLYYAVMSGFNE*TFVASSLMGKQHP

Sequence NM_001768

CGAAAAGGAGGGTGACTCTCCTCGGCGGGGGCTTCGGGTGACATCACATCCTCCAAATGCGAAATCAGGCTCCGGGCCGGCCGAAGGGCGCAACTTTCCC
CCCTCGGCGCCCCACCGGCTCCCGCGCGCCTCCCCTCGCGCCCGAGCTTCGAGCCAAGCAGCGTCCTGGGGAGCGCGTCATGGCCTTACCAGTGACCGCC
TTGCTCCTGCCGCTGGCCTTGCTGCTCCACGCCGCCAGGCCGAGCCAGTTCCGGGTGTCGCCGCTGGATCGGACCTGGAACCTGGGCGAGACAGTGGAGC
TGAAGTGCCAGGTGCTGCTGTCCAACCCGACGTCGGGCTGCTCGTGGCTCTTCCAGCCGCGCGGCGCCGCCGCCAGTCCCACCTTCCTCCTATACCTCTC
CCAAAACAAGCCCAAGGCGGCCGAGGGGCTGGACACCCAGCGGTTCTCGGGCAAGAGGTTGGGGGACACCTTCGTCCTCACCCTGAGCGACTTCCGCCGA
GAGAACGAGGGCTACTATTTCTGCTCGGCCCTGAGCAACTCCATCATGTACTTCAGCCACTTCGTGCCGGTCTTCCTGCCAGCGAAGCCCACCACGACGC
CAGCGCCGCGACCACCAACACCGGCGCCCACCATCGCGTCGCAGCCCCTGTCCCTGCGCCCAGAGGCGTGCCGGCCAGCGGCGGGGGGCGCAGTGCACAC
GAGGGGGCTGGACTTCGCCTGTGATATCTACATCTGGGCGCCCTTGGCCGGGACTTGTGGGGTCCTTCTCCTGTCACTGGTTATCACCCTTTACTGCAAC
CACAGGAACCGAAGACGTGTTTGCAAATGTCCCCGGCCTGTGGTCAAATCGGGAGACAAGCCCAGCCTTTCGGCGAGATACGTCTAACCCTGTGCAACAG
CCACTACATTACTTCAAACTGAGATCCTTCCTTTTGAGGGAGCAAGTCCTTCCCTTTCATTTTTTCCAGTCTTCCTCCCTGTGTATTCATTCTCATGATT
ATTATTTTAGTGGGGGCGGGGTGGGAAAGATTACTTTTTCTTTATGTGTTTGACGGGAAACAAAACTAGGTAAAATCTACAGTACACCACAAGGGTCACA
ATACTGTTGTGCGCACATCGCGGTAGGGCGTGGAAAGGGGCAGGCCAGAGCTACCCGCAGAGTTCTCAGAATCATGCTGAGAGAGCTGGAGGCACCCATG
CCATCTCAACCTCTTCCCCGCCCGTTTTACAAAGGGGGAGGCTAAAGCCCAGAGACAGCTTGATCAAAGGCACACAGCAAGTCAGGGTTGGAGCAGTAGC
TGGAGGGACCTTGTCTCCCAGCTCAGGGCTCTTTCCTCCACACCATTCAGGTCTTTCTTTCCGAGGCCCCTGTCTCAGGGTGAGGTGCTTGAGTCTCCAA
CGGCAAGGGAACAAGTACTTCTTGATACCTGGGATACTGTGCCCAGAGCCTCGAGGAGGTAATGAATTAAAGAAGAGAACTGCCTTTGGCAGAGTTCTAT
AATGTAAACAATATCAGACTTTTTTTTTTTATAATCAAGCCTAAAATTGTATAGACCTAAAATAAAATGAAGTGGTGAGCTTAACCCTGGAAAATGAATC
CCTCTATCTCTAAAGAAAATCTCTGTGAAACCCCTATGTGGAGGCGGAATTGCTCTCCCAGCCCTTGCATTGCAGAGGGGCCCATGAAAGAGGACAGGCT
ACCCCTTTACAAATAGAATTTGAGCATCAGTGAGGTTAAACTAAGGCCCTCTTGAATCTCTGAATTTGAGATACAAACATGTTCCTGGGATCACTGATGA
CTTTTTATACTTTGTAAAGACAATTGTTGGAGAGCCCCTCACACAGCCCTGGCCTCTGCTCAACTAGCAGATACAGGGATGAGGCAGACCTGACTCTCTT
AAGGAGGCTGAGAGCCCAAACTGCTGTCCCAAACATGCACTTCCTTGCTTAAGGTATGGTACAAGCAATGCCTGCCCATTGGAGAGAAAAAACTTAAGTA
GATAAGGAAATAAGAACCACTCATAATTCTTCACCTTAGGAATAATCTCCTGTTAATATGGTGTACATTCTTCCTGATTATTTTCTACACATACATGTAA
AATATGTCTTTCTTTTTTAAATAGGGTTGTACTATGCTGTTATGAGTGGCTTTAATGAATAAACATTTGTAGCATCCTCTTTAATGGGTAAACAGCATCC
GA
  • refseq_CD8A.F2 (forward primer)
  • refseq_CD8A.R2 (reverse primer)
  • Single exon skipping

Frame 0

RKGG*LSSAGASGDITSSKCEIRLRAGRRAQLS
PLGAPPAPARLPSRPSFEPSSVLGSASWPYQ*PP
CSCRWPCCSTPPGRASSGCRRWIGPGTWARQWS
*SARCCCPTRRRAARGSSSRAAPPPVPPSSYTS
PKTSPRRPRGWTPSGSRARGWGTPSSSP*ATSAE
RTRATISARP*ATPSCTSATSCRSSCQRSPPRR
QRRDHQHRRPPSRRSPCPCAQRRAGQRRGAQCT
RGGWTSPVISTSGRPWPGLVGSFSCHWLSPFTAT
TGTEDVFANVPGLWSNRETSPAFRRDTSNPVQQ
PLHYFKLRSFLLREQVLPFHFFQSSSLCIHSHD
YYFSGGGVGKITFSLCV*RETKLGKIYSTPQGSQ
YCCAHIAVGRGKGQARATRRVLRIMLRELEAPM
PSQPLPRPFYKGGG*SPETA*SKAHSKSGLEQ*
LEGPCLPAQGSFLHTIQVFLSEAPVSG*GA*VSN
GKGTSTS*YLGYCAQSLEEVMN*RRELPLAEFY
NVNNIRLFFFIIKPKIV*T*NKMKW*A*PWKMN
PSISKENLCETPMWRRNCSPSPCIAEGPMKEDRL
PLYK*NLSISEVKLRPS*ISEFEIQTCSWDH**
LFILCKDNCWRAPHTALASAQLADTGMRQT*LS
*GG*EPKLLSQTCTSLLKVWYKQCLPIGEKKLK*
IRK*EPLIILHLRNNLLLIWCTFFLIIFYTYM*
NMSFFFK*GCTMLL*VALMNKHL*HPL*WVNSI
R

Frame 1

EKEGDSPRRGLRVTSHPPNAKSGSGPAEGRNFP
PSAPHRLPRASPRARASSQAASWGARHGLTSDR
LAPAAGLAAPRRQAEPVPGVAAGSDLEPGRDSGA
EVPGAAVQPDVGLLVALPAARRRRQSHLPPIPL
PKQAQGGRGAGHPAVLGQEVGGHLRPHPERLPP
RERGLLFLLGPEQLHHVLQPLRAGLPASEAHHDA
SAATTNTGAHHRVAAPVPAPRGVPASGGGRSAH
EGAGLRL*YLHLGALGRDLWGPSPVTGYHPLLQ
PQEPKTCLQMSPACGQIGRQAQPFGEIRLTLCNS
HYITSN*DPSF*GSKSFPFIFSSLPPCVFILMI
IILVGAGWERLLFLYVFDGKQN*VKSTVHHKGH
NTVVRTSR*GVERGRPELPAEFSESC*ESWRHPC
HLNLFPARFTKGEAKAQRQLDQRHTASQGWSSS
WRDLVSQLRALSSTPFRSFFPRPLSQGEVLESP
TAREQVLLDTWDTVPRASRR**IKEENCLWQSSI
M*TISDFFFL*SSLKLYRPKIK*SGELNPGK*I
PLSLKKISVKPLCGGGIALPALALQRGP*KRTG
YPFTNRI*ASVRLN*GPLESLNLRYKHVPGITDD
FLYFVKTIVGEPLTQPWPLLN*QIQG*GRPDSL
KEAESPNCCPKHALPCLRYGTSNACPLERKNLS
R*GNKNHS*FFTLGIISC*YGVHSS*LFSTHTCK
ICLSFLNRVVLCCYEWL**INICSILFNG*TAS

Frame 2

KRRVTLLGGGFG*HHILQMRNQAPGRPKGATFP
PRRPTGSRAPPLAPELRAKQRPGERVMALPVTA
LLLPLALLLHAARPSQFRVSPLDRTWNLGETVE
LKCQVLLSNPTSGCSWLFQPRGAAASPTFLLYLS
QNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRR
ENEGYYFCSALSNSIMYFSHFVPVFLPAKPTTT
PAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHT
RGLDFACDIYIWAPLAGTCGVLLLSLVITLYCN
HRNRRRVCKCPRPVVKSGDKPSLSARYV*PCAT
ATTLLQTEILPFEGASPSLSFFPVFLPVYSFS*L
LF*WGRGGKDYFFFMCLTGNKTR*NLQYTTRVT
ILLCAHRGRAWKGAGQSYPQSSQNHAERAGGTH
AISTSSPPVLQRGRLKPRDSLIKGTQQVRVGAVA
GGTLSPSSGLFPPHHSGLSFRGPCLRVRCLSLQ
RQGNKYFLIPGILCPEPRGGNELKKRTAFGRVL
*CKQYQTFFFYNQA*NCIDLK*NEVVSLTLENES
LYL*RKSL*NPYVEAELLSQPLHCRGAHERGQA
TPLQIEFEHQ*G*TKALLNL*I*DTNMFLGSLM
TFYTL*RQLLESPSHSPGLCSTSRYRDEADLTLL
RRLRAQTAVPNMHFLA*GMVQAMPAHWREKT*V
DKEIRTTHNSSP*E*SPVNMVYILPDYFLHIHV
KYVFLF*IGLYYAVMSGFNE*TFVASSLMGKQHP

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