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Sequences

Sequence NM_080812

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
TTTTTCAGGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCAAAGGGCGACATGGG
TCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGCTGCTGCCTCTCCTCAATTCA
GTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTG
GACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGG
CTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAG
CCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACT
CCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGG
GCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGAT
TACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAG
GGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGG
TCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGA
CAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTC
CGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCT
GGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGG
GGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGG
GGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCA
AGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATG
CCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGT
TTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTG
TGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGT
GTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F4 (forward primer)
  • refseq_COL13A1.R4 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
FFRVNQELEVSLDFRVPLGWTANRATQDQRATW
V*RVPQDSRDPRDKKEKRVSVESTHTGCCLSSIQ
CDWLHPRS*KGGRSRANRARPASKVHQGPQAPL
DQVDLWGTQDCQGLWGHLAYLGLLDQRETQGSR
ATTAGRENGACQGCQASMEPRGRPELPWLG*RVS
QGSQEPRVRRGLKAPLGFLASWGRRERKAMLAT
PLEEAEGNLALQGSLGPQGQREKQVSMARLAPQ
GSQETRGSVEQLENRDQMAPRAPRENQGKERWWI
TMETSMRLSRRSGRWP*WGLLVFLGKLAHLELQ
GFQARRGRLDCQALQDTMGKRDLAVNQETWALL
VPKAPQERMDLQE*REKTGTQGAQERRGKKGRQD
KQAHRERKEKPGRRAIQEQRFLGCQGQRGLPDL
RGSKVFLDQRGKQD*MEQKERKASREKKETVVP
WDYPELQVWTAGLGHRVLQDQLEFQAQRDQRARG
AAKETLG*QDQREQLGFLVYMDHPGTRETGGRG
GRKALEGLKGIRETKERLD*MPPAHWAKMAYQS
KAAGTSDASNLGLACVCVCT*NIYFYTVFTF*KC
QKYDASYRLLKKKEKPAYFVQKISTSSLLFTRC
FV*AYVSNTFFVWS*CLHDICAHLLSIEA**II
VSWCQRGPARTEVLASSCVNSHKCRGP*YLLS*V
CLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
FSGSTRN*RFPWISGSHWAGRQTGPPRTKGRHG
SDGSPRTAGTPGTKRRKGSVWRVPTPAAASPQF
SATGSTPGHKKADVPGRTEPGQHPRSTRAPRPPW
TKWTSGAPRTARAYGATWLTWASWTKGRPRDPG
LPRPEGRTGHARDARQAWSQGGARNCRGWDEG*
ARDPRNQG*EGG*RLPWASWPPGAEGRERRCWQL
HWRRQRGTWPSRAPWAPRAKGRSRCRWPGWPPR
AARRQGGAWSSWRTGTRWPQGLQGRTRERRDGG
LQWKHQ*GSPGDPDAGLDGASWSSWANWPTWSSR
DSRPEGGDWTARPSRTRWGKGTSR*TRRHGPSW
SPRPPRKGWTSRSEGRKRAPREPRREGGKRGDR
TSRLTGRERRSRGEGQSRSRGSWAARARGASRTS
GAPRCSWTKGGSRTRWSKRRERLPGRKRRPWSP
GTTRSFRFGRQAWATGYSRTNWSSRPSGTKGRE
GQQRRPWDDRTNGSSWASWFTWTTRGQGKPGGEG
EERL*RA*RG*GRPRSAWIRCPLPIGRRWLTSP
RLLEQVMPLTLDWPVCVFVHRIFIFIQFSLFEN
ARSMMHLTDY*KRKKNLHILYRKYQPLPFCLQDV
LYKPMSLIHFLFGRNVCMIFVHIY*VSKLNKLL
CPGAKGGQPELRCWLAHV*IHINVEVHDIC*AR
CV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
VFQGQPGTRGFPGFPGPIGLDGKPGHPGPKGDMG
LTGPPGQPGPQGQKGEKGQCGEYPHRLLPLLNS
VRLAPPPVIKRRTFQGEQSQASIQGPPGPPGPP
GPSGPLGHPGLPGPMGPPGLPGPPGPKGDPGIQG
YHGRKGERGMPGMPGKHGAKGAPGIAVAGMKGE
PGIPGTKGEKGAEGSPGLPGLLGQKGEKGDAGN
SIGGGRGEPGPPGLPGPPGPKGEAGVDGQVGPPG
QPGDKGERGAAGEQGPDGPKGSKGEPGKGEMVD
YNGNINEALQEIRTLALMGPPGLPGQIGPPGAP
GIPGQKGEIGLPGPPGHDGEKGPRGKPGDMGPPG
PQGPPGKDGPPGVKGENGHPGSPGEKGEKGETG
QAGSPGEKGEAGEKGNPGAEVPGLPGPEGPPGP
PGLQGVPGPKGEAGLDGAKGEKGFQGEKGDRGPL
GLPGASGLDGRPGPPGTPGPIGVPGPAGPKGER
GSKGDPGMTGPTGAAGLPGLHGPPGDKGNRGER
GKKGSRGPKGDKGDQGAPGLDAPCPLGEDGLPVQ
GCWNK*CL*PWIGLCVCLYIEYLFLYSFHFLKM
PEV*CILQIIKKERKTCIFCTENINLFPFVYKM
FCISLCL*YIFCLVVMSA*YLCTFIKYRSLINYC
VLVPKGASQN*GAG*LMCEFT*M*RSMIFAKLG
VSKSILNPYGFSLLKEMKHGN

Sequence NM_080800

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGCTGCTGC
CTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCC
CCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGAC
CCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTG
GGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGG
CGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAG
GTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAG
GAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCC
ACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGAC
ATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAA
AAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGG
GCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGA
GACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGAC
CAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAA
CCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGAT
GGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTC
ACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTT
TGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGC
TTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATAT
TTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F4 (forward primer)
  • refseq_COL13A1.R4 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGCC
LSSIQCDWLHPRS*KGGRSRANRARPASKVHQG
PQAPLDQVDLWGTQDCQGLWGHLAYLGLLDQRET
QGSRATTAGRENGACQGCQASMEPRGRPELPWL
G*RVSQGSQEPRVRRGLKAPLGFLASWGRRERK
AMLATPLEEAEGNLALQGSLGPQGQREKQVSMAR
LAPQGSQETRGSVEQLENRDQMAPRAPRENQGK
ERWWITMETSMRLSRRSGRWP*WGLLVFLGKLA
HLELQGFQARRGRLDCQALQDTMGKRDLAVNQET
WALLVPKAPQERMDLQE*REKTGTQGAQERRGK
KGRQDKQAHRERKEKPGRRAIQEQRFLGCQGQR
GLPDLRGSKVFLDQRGKQD*MEQKERKASREKKE
TVVPWDYPELQVWTAGLGHRVLQDQLEFQAQRD
QRARGAAKETLG*QDQREQLGFLVYMDHPGTRE
TGGRGGRKALEGLKGIRETKERLD*MPPAHWAKM
AYQSKAAGTSDASNLGLACVCVCT*NIYFYTVF
TF*KCQKYDASYRLLKKKEKPAYFVQKISTSSL
LFTRCFV*AYVSNTFFVWS*CLHDICAHLLSIEA
**IIVSWCQRGPARTEVLASSCVNSHKCRGP*Y
LLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPAAA
SPQFSATGSTPGHKKADVPGRTEPGQHPRSTRA
PRPPWTKWTSGAPRTARAYGATWLTWASWTKGR
PRDPGLPRPEGRTGHARDARQAWSQGGARNCRGW
DEG*ARDPRNQG*EGG*RLPWASWPPGAEGRER
RCWQLHWRRQRGTWPSRAPWAPRAKGRSRCRWP
GWPPRAARRQGGAWSSWRTGTRWPQGLQGRTRER
RDGGLQWKHQ*GSPGDPDAGLDGASWSSWANWP
TWSSRDSRPEGGDWTARPSRTRWGKGTSR*TRR
HGPSWSPRPPRKGWTSRSEGRKRAPREPRREGGK
RGDRTSRLTGRERRSRGEGQSRSRGSWAARARG
ASRTSGAPRCSWTKGGSRTRWSKRRERLPGRKR
RPWSPGTTRSFRFGRQAWATGYSRTNWSSRPSGT
KGREGQQRRPWDDRTNGSSWASWFTWTTRGQGK
PGGEGEERL*RA*RG*GRPRSAWIRCPLPIGRR
WLTSPRLLEQVMPLTLDWPVCVFVHRIFIFIQFS
LFENARSMMHLTDY*KRKKNLHILYRKYQPLPF
CLQDVLYKPMSLIHFLFGRNVCMIFVHIY*VSK
LNKLLCPGAKGGQPELRCWLAHV*IHINVEVHDI
C*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHRLL
PLLNSVRLAPPPVIKRRTFQGEQSQASIQGPPGP
PGPPGPSGPLGHPGLPGPMGPPGLPGPPGPKGD
PGIQGYHGRKGERGMPGMPGKHGAKGAPGIAVA
GMKGEPGIPGTKGEKGAEGSPGLPGLLGQKGEKG
DAGNSIGGGRGEPGPPGLPGPPGPKGEAGVDGQ
VGPPGQPGDKGERGAAGEQGPDGPKGSKGEPGK
GEMVDYNGNINEALQEIRTLALMGPPGLPGQIGP
PGAPGIPGQKGEIGLPGPPGHDGEKGPRGKPGD
MGPPGPQGPPGKDGPPGVKGENGHPGSPGEKGE
KGETGQAGSPGEKGEAGEKGNPGAEVPGLPGPEG
PPGPPGLQGVPGPKGEAGLDGAKGEKGFQGEKG
DRGPLGLPGASGLDGRPGPPGTPGPIGVPGPAG
PKGERGSKGDPGMTGPTGAAGLPGLHGPPGDKGN
RGERGKKGSRGPKGDKGDQGAPGLDAPCPLGED
GLPVQGCWNK*CL*PWIGLCVCLYIEYLFLYSF
HFLKMPEV*CILQIIKKERKTCIFCTENINLFPF
VYKMFCISLCL*YIFCLVVMSA*YLCTFIKYRS
LINYCVLVPKGASQN*GAG*LMCEFT*M*RSMI
FAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080807

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGCTGCTGC
CTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCC
CCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGAC
CCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTG
GGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGG
CGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAG
GTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAG
GAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCC
ACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGAC
ATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAA
AAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGG
GCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGA
GACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGA
TGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCC
TAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGC
CTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTAC
AGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTA
ATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCC
AGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACC
CTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F4 (forward primer)
  • refseq_COL13A1.R4 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGCC
LSSIQCDWLHPRS*KGGRSRANRARPASKVHQG
PQAPLDQVDLWGTQDCQGLWGHLAYLGLLDQRET
QGSRATTAGRENGACQGCQASMEPRGRPELPWL
G*RVSQGSQEPRVRRGLKAPLGFLASWGRRERK
AMLATPLEEAEGNLALQGSLGPQGQREKQVSMAR
LAPQGSQETRGSVEQLENRDQMAPRAPRENQGK
ERWWITMETSMRLSRRSGRWP*WGLLVFLGKLA
HLELQGFQARRGRLDCQALQDTMGKRDLAVNQET
WALLVPKAPQERMDLQE*REKTGTQGAQERRGK
KGRQDKQAHRERKEKPGRRAIQEQRFLGCQGQR
GLPDLRGSKVFLDQRGKQD*MEQKERKASREKKE
TVVPWDYPVLQDQLEFQAQRDQRARGAAKETLG
*QDQREQLGFLVYMDHPGTRETGGRGGRKALEG
LKGIRETKERLD*MPPAHWAKMAYQSKAAGTSDA
SNLGLACVCVCT*NIYFYTVFTF*KCQKYDASY
RLLKKKEKPAYFVQKISTSSLLFTRCFV*AYVS
NTFFVWS*CLHDICAHLLSIEA**IIVSWCQRGP
ARTEVLASSCVNSHKCRGP*YLLS*VCLRVF*T
LMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPAAA
SPQFSATGSTPGHKKADVPGRTEPGQHPRSTRA
PRPPWTKWTSGAPRTARAYGATWLTWASWTKGR
PRDPGLPRPEGRTGHARDARQAWSQGGARNCRGW
DEG*ARDPRNQG*EGG*RLPWASWPPGAEGRER
RCWQLHWRRQRGTWPSRAPWAPRAKGRSRCRWP
GWPPRAARRQGGAWSSWRTGTRWPQGLQGRTRER
RDGGLQWKHQ*GSPGDPDAGLDGASWSSWANWP
TWSSRDSRPEGGDWTARPSRTRWGKGTSR*TRR
HGPSWSPRPPRKGWTSRSEGRKRAPREPRREGGK
RGDRTSRLTGRERRSRGEGQSRSRGSWAARARG
ASRTSGAPRCSWTKGGSRTRWSKRRERLPGRKR
RPWSPGTTRYSRTNWSSRPSGTKGREGQQRRPWD
DRTNGSSWASWFTWTTRGQGKPGGEGEERL*RA
*RG*GRPRSAWIRCPLPIGRRWLTSPRLLEQVM
PLTLDWPVCVFVHRIFIFIQFSLFENARSMMHLT
DY*KRKKNLHILYRKYQPLPFCLQDVLYKPMSL
IHFLFGRNVCMIFVHIY*VSKLNKLLCPGAKGG
QPELRCWLAHV*IHINVEVHDIC*ARCV*EYFKP
LWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHRLL
PLLNSVRLAPPPVIKRRTFQGEQSQASIQGPPGP
PGPPGPSGPLGHPGLPGPMGPPGLPGPPGPKGD
PGIQGYHGRKGERGMPGMPGKHGAKGAPGIAVA
GMKGEPGIPGTKGEKGAEGSPGLPGLLGQKGEKG
DAGNSIGGGRGEPGPPGLPGPPGPKGEAGVDGQ
VGPPGQPGDKGERGAAGEQGPDGPKGSKGEPGK
GEMVDYNGNINEALQEIRTLALMGPPGLPGQIGP
PGAPGIPGQKGEIGLPGPPGHDGEKGPRGKPGD
MGPPGPQGPPGKDGPPGVKGENGHPGSPGEKGE
KGETGQAGSPGEKGEAGEKGNPGAEVPGLPGPEG
PPGPPGLQGVPGPKGEAGLDGAKGEKGFQGEKG
DRGPLGLPGTPGPIGVPGPAGPKGERGSKGDPG
MTGPTGAAGLPGLHGPPGDKGNRGERGKKGSRGP
KGDKGDQGAPGLDAPCPLGEDGLPVQGCWNK*C
L*PWIGLCVCLYIEYLFLYSFHFLKMPEV*CIL
QIIKKERKTCIFCTENINLFPFVYKMFCISLCL*
YIFCLVVMSA*YLCTFIKYRSLINYCVLVPKGA
SQN*GAG*LMCEFT*M*RSMIFAKLGVSKSILN
PYGFSLLKEMKHGN

Sequence NM_080809

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA
AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA
CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA
TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA
GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC
CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG
CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA
GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA
CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC
CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGTTCCTGGGCTGCC
AGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAG
GGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAG
GAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGG
CTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGG
AACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTAT
GATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAG
CCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTG
CCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGA
GTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F4 (forward primer)
  • refseq_COL13A1.R4 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS*
KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD
CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA
CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR
RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA
LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE
QLENRDQMAPRAPRENQGKERWWITMETSMRLS
RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD
CQALQDTMGKRDLAVNQETWALLVPKAPQERMD
LQE*REKTGTQGAQERRGKKGRQDKQAHRFLGC
QGQRGLPDLRGSKVFLDQRGKQD*MEQKERKASR
EKKETVVPWDYPVLQDQLEFQAQRDQRARGAAK
ETLG*QDQREQLGFLVYMDHPGTRETGGRGGRK
ALEGLKGIRETKERLD*MPPAHWAKMAYQSKAAG
TSDASNLGLACVCVCT*NIYFYTVFTF*KCQKY
DASYRLLKKKEKPAYFVQKISTSSLLFTRCFV*
AYVSNTFFVWS*CLHDICAHLLSIEA**IIVSWC
QRGPARTEVLASSCVNSHKCRGP*YLLS*VCLR
VF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEAAASPQFSATGSTPGHK
KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR
TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH
ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E
GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW
PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS
SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP
GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW
TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT
SRSEGRKRAPREPRREGGKRGDRTSRLTGSWAA
RARGASRTSGAPRCSWTKGGSRTRWSKRRERLP
GRKRRPWSPGTTRYSRTNWSSRPSGTKGREGQQR
RPWDDRTNGSSWASWFTWTTRGQGKPGGEGEER
L*RA*RG*GRPRSAWIRCPLPIGRRWLTSPRLL
EQVMPLTLDWPVCVFVHRIFIFIQFSLFENARSM
MHLTDY*KRKKNLHILYRKYQPLPFCLQDVLYK
PMSLIHFLFGRNVCMIFVHIY*VSKLNKLLCPG
AKGGQPELRCWLAHV*IHINVEVHDIC*ARCV*E
YFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK
RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG
LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG
MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK
GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG
PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG
AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ
EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG
LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG
PPGVKGENGHPGSPGEKGEKGETGQAGSPVPGLP
GPEGPPGPPGLQGVPGPKGEAGLDGAKGEKGFQ
GEKGDRGPLGLPGTPGPIGVPGPAGPKGERGSK
GDPGMTGPTGAAGLPGLHGPPGDKGNRGERGKKG
SRGPKGDKGDQGAPGLDAPCPLGEDGLPVQGCW
NK*CL*PWIGLCVCLYIEYLFLYSFHFLKMPEV
*CILQIIKKERKTCIFCTENINLFPFVYKMFCIS
LCL*YIFCLVVMSA*YLCTFIKYRSLINYCVLV
PKGASQN*GAG*LMCEFT*M*RSMIFAKLGVSK
SILNPYGFSLLKEMKHGN

Sequence NM_080798

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGTCCCCCGGAGACGCTGGGCTGTCCATCATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAAC
TAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCAAAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAG
CCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCCTAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAA
TAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAG
CATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGG
CCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGG
CGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCT
GGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGA
GAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCT
CCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGG
TCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGA
CCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGA
GCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCC
TGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAA
GGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTG
GAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGG
ACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCC
CCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAAT
ATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGA
AAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGC
ACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACAT
AAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F4 (forward primer)
  • refseq_COL13A1.R4 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGPPETLGCPSLVPAAPLVNQE
LEVSLDFRVPLGWTANRATQDQRATWV*RVPQDS
RDPRDKKEKRVSVESTHTGSA*AACQQLCAPAK
*LP*SCCLSSIQCDWLHPRS*KGGRSRANRARP
ASKVHQGPQAPLDQVDLWGTQDCQGLWGHLAYLG
LLDQRETQGSRATTAGRENGACQGCQASMEPRG
RPELPWLG*RVSQGSQEPRVRRGLKAPLGFLAS
WGRRERKAMLATPLEEAEGNLALQGSLGPQGQRE
KQVSMARLAPQGSQETRGSVEQLENRDQMAPRA
PRENQGKERWWITMETSMRLSRRSGRWP*WGLL
VFLGKLAHLELQGFQARRGRLDCQALQDTMGKRD
LAVNQETWALLVPKAPQERMDLQE*REKTGTQG
AQERRGKKGRQDKQAHRERKEKPGRRAIQEQRF
LGCQGQRGLPDLRGSKVFLDQRGKQD*MEQKERK
ASREKKETVVPWDYPELQVWTAGLGHRVLQDQL
EFQAQRDQRARGAAKETLG*QDQREQLGFLVYM
DHPGTRETGGRGGRKALEGLKGIRETKERLD*MP
PAHWAKMAYQSKAAGTSDASNLGLACVCVCT*N
IYFYTVFTF*KCQKYDASYRLLKKKEKPAYFVQ
KISTSSLLFTRCFV*AYVSNTFFVWS*CLHDICA
HLLSIEA**IIVSWCQRGPARTEVLASSCVNSH
KCRGP*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRVPRRRWAVHHWSPRPPWSTRN
*RFPWISGSHWAGRQTGPPRTKGRHGSDGSPRT
AGTPGTKRRKGSVWRVPTPGVPKQHASSSALQPN
NCPEAAASPQFSATGSTPGHKKADVPGRTEPGQ
HPRSTRAPRPPWTKWTSGAPRTARAYGATWLTW
ASWTKGRPRDPGLPRPEGRTGHARDARQAWSQGG
ARNCRGWDEG*ARDPRNQG*EGG*RLPWASWPP
GAEGRERRCWQLHWRRQRGTWPSRAPWAPRAKG
RSRCRWPGWPPRAARRQGGAWSSWRTGTRWPQGL
QGRTRERRDGGLQWKHQ*GSPGDPDAGLDGASW
SSWANWPTWSSRDSRPEGGDWTARPSRTRWGKG
TSR*TRRHGPSWSPRPPRKGWTSRSEGRKRAPRE
PRREGGKRGDRTSRLTGRERRSRGEGQSRSRGS
WAARARGASRTSGAPRCSWTKGGSRTRWSKRRE
RLPGRKRRPWSPGTTRSFRFGRQAWATGYSRTNW
SSRPSGTKGREGQQRRPWDDRTNGSSWASWFTW
TTRGQGKPGGEGEERL*RA*RG*GRPRSAWIRC
PLPIGRRWLTSPRLLEQVMPLTLDWPVCVFVHRI
FIFIQFSLFENARSMMHLTDY*KRKKNLHILYR
KYQPLPFCLQDVLYKPMSLIHFLFGRNVCMIFV
HIY*VSKLNKLLCPGAKGGQPELRCWLAHV*IHI
NVEVHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGSPGDAGLSIIGPRGPPGQPGT
RGFPGFPGPIGLDGKPGHPGPKGDMGLTGPPGQ
PGPQGQKGEKGQCGEYPHRECLSSMPAALRSSQ
IIALKLLPLLNSVRLAPPPVIKRRTFQGEQSQAS
IQGPPGPPGPPGPSGPLGHPGLPGPMGPPGLPG
PPGPKGDPGIQGYHGRKGERGMPGMPGKHGAKG
APGIAVAGMKGEPGIPGTKGEKGAEGSPGLPGLL
GQKGEKGDAGNSIGGGRGEPGPPGLPGPPGPKG
EAGVDGQVGPPGQPGDKGERGAAGEQGPDGPKG
SKGEPGKGEMVDYNGNINEALQEIRTLALMGPPG
LPGQIGPPGAPGIPGQKGEIGLPGPPGHDGEKG
PRGKPGDMGPPGPQGPPGKDGPPGVKGENGHPG
SPGEKGEKGETGQAGSPGEKGEAGEKGNPGAEVP
GLPGPEGPPGPPGLQGVPGPKGEAGLDGAKGEK
GFQGEKGDRGPLGLPGASGLDGRPGPPGTPGPI
GVPGPAGPKGERGSKGDPGMTGPTGAAGLPGLHG
PPGDKGNRGERGKKGSRGPKGDKGDQGAPGLDA
PCPLGEDGLPVQGCWNK*CL*PWIGLCVCLYIE
YLFLYSFHFLKMPEV*CILQIIKKERKTCIFCTE
NINLFPFVYKMFCISLCL*YIFCLVVMSA*YLC
TFIKYRSLINYCVLVPKGASQN*GAG*LMCEFT
*M*RSMIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080808

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA
AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA
CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA
TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA
GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC
CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG
CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA
GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA
CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC
CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGA
AGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTC
CAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCA
AAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAA
AGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGC
TGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAG
TATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTAT
AAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTG
GTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTA
AGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F4 (forward primer)
  • refseq_COL13A1.R4 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS*
KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD
CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA
CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR
RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA
LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE
QLENRDQMAPRAPRENQGKERWWITMETSMRLS
RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD
CQALQDTMGKRDLAVNQETWALLVPKAPQERMD
LQE*REKTGTQGAQERRGKKGRQDKQAHRERKE
KPGRRAIQEQRGSKVFLDQRGKQD*MEQKERKAS
REKKETVVPWDYPVLQDQLEFQAQRDQRARGAA
KETLG*QDQREQLGFLVYMDHPGTRETGGRGGR
KALEGLKGIRETKERLD*MPPAHWAKMAYQSKAA
GTSDASNLGLACVCVCT*NIYFYTVFTF*KCQK
YDASYRLLKKKEKPAYFVQKISTSSLLFTRCFV
*AYVSNTFFVWS*CLHDICAHLLSIEA**IIVSW
CQRGPARTEVLASSCVNSHKCRGP*YLLS*VCL
RVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEAAASPQFSATGSTPGHK
KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR
TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH
ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E
GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW
PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS
SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP
GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW
TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT
SRSEGRKRAPREPRREGGKRGDRTSRLTGRERR
SRGEGQSRSRGAPRCSWTKGGSRTRWSKRRERL
PGRKRRPWSPGTTRYSRTNWSSRPSGTKGREGQQ
RRPWDDRTNGSSWASWFTWTTRGQGKPGGEGEE
RL*RA*RG*GRPRSAWIRCPLPIGRRWLTSPRL
LEQVMPLTLDWPVCVFVHRIFIFIQFSLFENARS
MMHLTDY*KRKKNLHILYRKYQPLPFCLQDVLY
KPMSLIHFLFGRNVCMIFVHIY*VSKLNKLLCP
GAKGGQPELRCWLAHV*IHINVEVHDIC*ARCV*
EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK
RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG
LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG
MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK
GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG
PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG
AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ
EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG
LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG
PPGVKGENGHPGSPGEKGEKGETGQAGSPGEKGE
AGEKGNPGAEGLQGVPGPKGEAGLDGAKGEKGF
QGEKGDRGPLGLPGTPGPIGVPGPAGPKGERGS
KGDPGMTGPTGAAGLPGLHGPPGDKGNRGERGKK
GSRGPKGDKGDQGAPGLDAPCPLGEDGLPVQGC
WNK*CL*PWIGLCVCLYIEYLFLYSFHFLKMPE
V*CILQIIKKERKTCIFCTENINLFPFVYKMFCI
SLCL*YIFCLVVMSA*YLCTFIKYRSLINYCVL
VPKGASQN*GAG*LMCEFT*M*RSMIFAKLGVS
KSILNPYGFSLLKEMKHGN

Sequence NM_080799

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGTCAACCAGGAACTAGAGGTTTCCCTGG
ATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCAAAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGA
CAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCCTAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGC
TGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACC
AGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAG
GGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCG
TGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGA
GAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGAT
GGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAG
GGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAAT
TGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCA
GGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGG
GGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCC
AGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAA
AAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAG
CGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAA
GGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGC
GAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACA
GTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTT
CCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTAT
CGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCA
TGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F4 (forward primer)
  • refseq_COL13A1.R4 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWVNQELEVSL
DFRVPLGWTANRATQDQRATWV*RVPQDSRDPRD
KKEKRVSVESTHTGSA*AACQQLCAPAK*LP*S
CCLSSIQCDWLHPRS*KGGRSRANRARPASKVH
QGPQAPLDQVDLWGTQDCQGLWGHLAYLGLLDQR
ETQGSRATTAGRENGACQGCQASMEPRGRPELP
WLG*RVSQGSQEPRVRRGLKAPLGFLASWGRRE
RKAMLATPLEEAEGNLALQGSLGPQGQREKQVSM
ARLAPQGSQETRGSVEQLENRDQMAPRAPRENQ
GKERWWITMETSMRLSRRSGRWP*WGLLVFLGK
LAHLELQGFQARRGRLDCQALQDTMGKRDLAVNQ
ETWALLVPKAPQERMDLQE*REKTGTQGAQERR
GKKGRQDKQAHRERKEKPGRRAIQEQRFLGCQG
QRGLPDLRGSKVFLDQRGKQD*MEQKERKASREK
KETVVPWDYPELQVWTAGLGHRVLQDQLEFQAQ
RDQRARGAAKETLG*QDQREQLGFLVYMDHPGT
RETGGRGGRKALEGLKGIRETKERLD*MPPAHWA
KMAYQSKAAGTSDASNLGLACVCVCT*NIYFYT
VFTF*KCQKYDASYRLLKKKEKPAYFVQKISTS
SLLFTRCFV*AYVSNTFFVWS*CLHDICAHLLSI
EA**IIVSWCQRGPARTEVLASSCVNSHKCRGP
*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGSTRN*RFPW
ISGSHWAGRQTGPPRTKGRHGSDGSPRTAGTPG
TKRRKGSVWRVPTPGVPKQHASSSALQPNNCPEA
AASPQFSATGSTPGHKKADVPGRTEPGQHPRST
RAPRPPWTKWTSGAPRTARAYGATWLTWASWTK
GRPRDPGLPRPEGRTGHARDARQAWSQGGARNCR
GWDEG*ARDPRNQG*EGG*RLPWASWPPGAEGR
ERRCWQLHWRRQRGTWPSRAPWAPRAKGRSRCR
WPGWPPRAARRQGGAWSSWRTGTRWPQGLQGRTR
ERRDGGLQWKHQ*GSPGDPDAGLDGASWSSWAN
WPTWSSRDSRPEGGDWTARPSRTRWGKGTSR*T
RRHGPSWSPRPPRKGWTSRSEGRKRAPREPRREG
GKRGDRTSRLTGRERRSRGEGQSRSRGSWAARA
RGASRTSGAPRCSWTKGGSRTRWSKRRERLPGR
KRRPWSPGTTRSFRFGRQAWATGYSRTNWSSRPS
GTKGREGQQRRPWDDRTNGSSWASWFTWTTRGQ
GKPGGEGEERL*RA*RG*GRPRSAWIRCPLPIG
RRWLTSPRLLEQVMPLTLDWPVCVFVHRIFIFIQ
FSLFENARSMMHLTDY*KRKKNLHILYRKYQPL
PFCLQDVLYKPMSLIHFLFGRNVCMIFVHIY*V
SKLNKLLCPGAKGGQPELRCWLAHV*IHINVEVH
DIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGQPGTRGFPG
FPGPIGLDGKPGHPGPKGDMGLTGPPGQPGPQG
QKGEKGQCGEYPHRECLSSMPAALRSSQIIALK
LLPLLNSVRLAPPPVIKRRTFQGEQSQASIQGPP
GPPGPPGPSGPLGHPGLPGPMGPPGLPGPPGPK
GDPGIQGYHGRKGERGMPGMPGKHGAKGAPGIA
VAGMKGEPGIPGTKGEKGAEGSPGLPGLLGQKGE
KGDAGNSIGGGRGEPGPPGLPGPPGPKGEAGVD
GQVGPPGQPGDKGERGAAGEQGPDGPKGSKGEP
GKGEMVDYNGNINEALQEIRTLALMGPPGLPGQI
GPPGAPGIPGQKGEIGLPGPPGHDGEKGPRGKP
GDMGPPGPQGPPGKDGPPGVKGENGHPGSPGEK
GEKGETGQAGSPGEKGEAGEKGNPGAEVPGLPGP
EGPPGPPGLQGVPGPKGEAGLDGAKGEKGFQGE
KGDRGPLGLPGASGLDGRPGPPGTPGPIGVPGP
AGPKGERGSKGDPGMTGPTGAAGLPGLHGPPGDK
GNRGERGKKGSRGPKGDKGDQGAPGLDAPCPLG
EDGLPVQGCWNK*CL*PWIGLCVCLYIEYLFLY
SFHFLKMPEV*CILQIIKKERKTCIFCTENINLF
PFVYKMFCISLCL*YIFCLVVMSA*YLCTFIKY
RSLINYCVLVPKGASQN*GAG*LMCEFT*M*RS
MIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_005203

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA
AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA
CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA
TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA
GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC
CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG
CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA
GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA
CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC
CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGA
AGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAG
GGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACG
GCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGG
ACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGG
GATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAAC
CTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTAT
TAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATT
TTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGA
ACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGG
ATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F4 (forward primer)
  • refseq_COL13A1.R4 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS*
KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD
CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA
CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR
RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA
LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE
QLENRDQMAPRAPRENQGKERWWITMETSMRLS
RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD
CQALQDTMGKRDLAVNQETWALLVPKAPQERMD
LQE*REKTGTQGAQERRGKKGRQDKQAHRERKE
KPGRRAIQEQRFLGCQGQRGLPDLRGSKVFLDQR
GKQD*MEQKERKASREKKETVVPWDYPELQVWT
AGLGHRVLQDQLEFQAQRDQRARGAAKETLG*Q
DQREQLGFLVYMDHPGTRETGGRGGRKALEGLKG
IRETKERLD*MPPAHWAKMAYQSKAAGTSDASN
LGLACVCVCT*NIYFYTVFTF*KCQKYDASYRL
LKKKEKPAYFVQKISTSSLLFTRCFV*AYVSNTF
FVWS*CLHDICAHLLSIEA**IIVSWCQRGPAR
TEVLASSCVNSHKCRGP*YLLS*VCLRVF*TLM
DFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEAAASPQFSATGSTPGHK
KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR
TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH
ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E
GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW
PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS
SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP
GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW
TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT
SRSEGRKRAPREPRREGGKRGDRTSRLTGRERR
SRGEGQSRSRGSWAARARGASRTSGAPRCSWTK
GGSRTRWSKRRERLPGRKRRPWSPGTTRSFRFGR
QAWATGYSRTNWSSRPSGTKGREGQQRRPWDDR
TNGSSWASWFTWTTRGQGKPGGEGEERL*RA*R
G*GRPRSAWIRCPLPIGRRWLTSPRLLEQVMPLT
LDWPVCVFVHRIFIFIQFSLFENARSMMHLTDY
*KRKKNLHILYRKYQPLPFCLQDVLYKPMSLIH
FLFGRNVCMIFVHIY*VSKLNKLLCPGAKGGQPE
LRCWLAHV*IHINVEVHDIC*ARCV*EYFKPLW
IFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK
RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG
LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG
MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK
GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG
PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG
AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ
EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG
LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG
PPGVKGENGHPGSPGEKGEKGETGQAGSPGEKGE
AGEKGNPGAEVPGLPGPEGPPGPPGLQGVPGPK
GEAGLDGAKGEKGFQGEKGDRGPLGLPGASGLD
GRPGPPGTPGPIGVPGPAGPKGERGSKGDPGMTG
PTGAAGLPGLHGPPGDKGNRGERGKKGSRGPKG
DKGDQGAPGLDAPCPLGEDGLPVQGCWNK*CL*
PWIGLCVCLYIEYLFLYSFHFLKMPEV*CILQII
KKERKTCIFCTENINLFPFVYKMFCISLCL*YI
FCLVVMSA*YLCTFIKYRSLINYCVLVPKGASQ
N*GAG*LMCEFT*M*RSMIFAKLGVSKSILNPYG
FSLLKEMKHGN

Sequence NM_080802

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA
AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA
CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA
TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA
GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC
CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG
CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA
GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA
CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC
CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGTTCCTGGGCTGCC
AGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAG
GGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAG
GCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGG
GGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCA
TTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTT
TATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAA
CCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATT
AAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGA
GGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F4 (forward primer)
  • refseq_COL13A1.R4 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS*
KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD
CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA
CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR
RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA
LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE
QLENRDQMAPRAPRENQGKERWWITMETSMRLS
RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD
CQALQDTMGKRDLAVNQETWALLVPKAPQERMD
LQE*REKTGTQGAQERRGKKGRQDKQAHRFLGC
QGQRGLPDLRGSKVFLDQRGKQD*MEQKERKASR
EKKETVVPWDYPELQVWTAGLGHRVLQDQLEFQ
AQRDQRARGAAKETLG*QDQREQLGFLVYMDHP
GTRETGGRGGRKALEGLKGIRETKERLD*MPPAH
WAKMAYQSKAAGTSDASNLGLACVCVCT*NIYF
YTVFTF*KCQKYDASYRLLKKKEKPAYFVQKIS
TSSLLFTRCFV*AYVSNTFFVWS*CLHDICAHLL
SIEA**IIVSWCQRGPARTEVLASSCVNSHKCR
GP*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEAAASPQFSATGSTPGHK
KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR
TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH
ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E
GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW
PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS
SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP
GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW
TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT
SRSEGRKRAPREPRREGGKRGDRTSRLTGSWAA
RARGASRTSGAPRCSWTKGGSRTRWSKRRERLP
GRKRRPWSPGTTRSFRFGRQAWATGYSRTNWSSR
PSGTKGREGQQRRPWDDRTNGSSWASWFTWTTR
GQGKPGGEGEERL*RA*RG*GRPRSAWIRCPLP
IGRRWLTSPRLLEQVMPLTLDWPVCVFVHRIFIF
IQFSLFENARSMMHLTDY*KRKKNLHILYRKYQ
PLPFCLQDVLYKPMSLIHFLFGRNVCMIFVHIY
*VSKLNKLLCPGAKGGQPELRCWLAHV*IHINVE
VHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK
RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG
LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG
MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK
GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG
PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG
AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ
EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG
LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG
PPGVKGENGHPGSPGEKGEKGETGQAGSPVPGLP
GPEGPPGPPGLQGVPGPKGEAGLDGAKGEKGFQ
GEKGDRGPLGLPGASGLDGRPGPPGTPGPIGVP
GPAGPKGERGSKGDPGMTGPTGAAGLPGLHGPPG
DKGNRGERGKKGSRGPKGDKGDQGAPGLDAPCP
LGEDGLPVQGCWNK*CL*PWIGLCVCLYIEYLF
LYSFHFLKMPEV*CILQIIKKERKTCIFCTENIN
LFPFVYKMFCISLCL*YIFCLVVMSA*YLCTFI
KYRSLINYCVLVPKGASQN*GAG*LMCEFT*M*
RSMIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080805

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGG
CCACCCAGGACCAAAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCA
CACCGGGAGTGCCTAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCAC
CCCCGGTCATAAAAAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCT
GGGGCACCCAGGACTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAG
GGAGAACGGGGCATGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAA
CCAAGGGTGAGAAGGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAG
AGGGGAACCTGGCCCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAG
GGGGAGCGTGGAGCAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCA
ATGAGGCTCTCCAGGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAA
GGGGGAGATTGGACTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCA
GGAAAGGATGGACCTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGG
GAGAGAAAGGAGAAGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGT
TCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCT
TCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACC
CTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAG
AGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAG
TGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCA
TCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATG
TCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAG
GGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTT
TAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F4 (forward primer)
  • refseq_COL13A1.R4 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQVNQELEVSLDFRVPLGWTANR
ATQDQRATWV*RVPQDSRDPRDKKEKRVSVESTH
TGSA*AACQQLCAPAK*LP*SCCLSSIQCDWLH
PRS*KGGRSRANRARPASKVHQGPQAPLDQVDL
WGTQDCQGLWGHLAYLGLLDQRETQGSRATTAGR
ENGACQGCQASMEPRGRPELPWLG*RVSQGSQE
PRVRRGLKAPLGFLASWGRRERKAMLATPLEEA
EGNLALQGSLGPQGQREKQVSMARLAPQGSQETR
GSVEQLENRDQMAPRAPRENQGKERWWITMETS
MRLSRRSGRWP*WGLLVFLGKLAHLELQGFQAR
RGRLDCQALQDTMGKRDLAVNQETWALLVPKAPQ
ERMDLQE*REKTGTQGAQERRGKKGRQDKQAHR
ERKEKPGRRAIQEQRFLGCQGQRGLPDLRGSKV
FLDQRGKQD*MEQKERKASREKKETVVPWDYPEL
QVWTAGLGHRVLQDQLEFQAQRDQRARGAAKET
LG*QDQREQLGFLVYMDHPGTRETGGRGGRKAL
EGLKGIRETKERLD*MPPAHWAKMAYQSKAAGTS
DASNLGLACVCVCT*NIYFYTVFTF*KCQKYDA
SYRLLKKKEKPAYFVQKISTSSLLFTRCFV*AY
VSNTFFVWS*CLHDICAHLLSIEA**IIVSWCQR
GPARTEVLASSCVNSHKCRGP*YLLS*VCLRVF
*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRSTRN*RFPWISGSHWAGRQTG
PPRTKGRHGSDGSPRTAGTPGTKRRKGSVWRVP
TPGVPKQHASSSALQPNNCPEAAASPQFSATGST
PGHKKADVPGRTEPGQHPRSTRAPRPPWTKWTS
GAPRTARAYGATWLTWASWTKGRPRDPGLPRPE
GRTGHARDARQAWSQGGARNCRGWDEG*ARDPRN
QG*EGG*RLPWASWPPGAEGRERRCWQLHWRRQ
RGTWPSRAPWAPRAKGRSRCRWPGWPPRAARRQ
GGAWSSWRTGTRWPQGLQGRTRERRDGGLQWKHQ
*GSPGDPDAGLDGASWSSWANWPTWSSRDSRPE
GGDWTARPSRTRWGKGTSR*TRRHGPSWSPRPP
RKGWTSRSEGRKRAPREPRREGGKRGDRTSRLTG
RERRSRGEGQSRSRGSWAARARGASRTSGAPRC
SWTKGGSRTRWSKRRERLPGRKRRPWSPGTTRS
FRFGRQAWATGYSRTNWSSRPSGTKGREGQQRRP
WDDRTNGSSWASWFTWTTRGQGKPGGEGEERL*
RA*RG*GRPRSAWIRCPLPIGRRWLTSPRLLEQ
VMPLTLDWPVCVFVHRIFIFIQFSLFENARSMMH
LTDY*KRKKNLHILYRKYQPLPFCLQDVLYKPM
SLIHFLFGRNVCMIFVHIY*VSKLNKLLCPGAK
GGQPELRCWLAHV*IHINVEVHDIC*ARCV*EYF
KPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGQPGTRGFPGFPGPIGLDGKPG
HPGPKGDMGLTGPPGQPGPQGQKGEKGQCGEYP
HRECLSSMPAALRSSQIIALKLLPLLNSVRLAP
PPVIKRRTFQGEQSQASIQGPPGPPGPPGPSGPL
GHPGLPGPMGPPGLPGPPGPKGDPGIQGYHGRK
GERGMPGMPGKHGAKGAPGIAVAGMKGEPGIPG
TKGEKGAEGSPGLPGLLGQKGEKGDAGNSIGGGR
GEPGPPGLPGPPGPKGEAGVDGQVGPPGQPGDK
GERGAAGEQGPDGPKGSKGEPGKGEMVDYNGNI
NEALQEIRTLALMGPPGLPGQIGPPGAPGIPGQK
GEIGLPGPPGHDGEKGPRGKPGDMGPPGPQGPP
GKDGPPGVKGENGHPGSPGEKGEKGETGQAGSP
GEKGEAGEKGNPGAEVPGLPGPEGPPGPPGLQGV
PGPKGEAGLDGAKGEKGFQGEKGDRGPLGLPGA
SGLDGRPGPPGTPGPIGVPGPAGPKGERGSKGD
PGMTGPTGAAGLPGLHGPPGDKGNRGERGKKGSR
GPKGDKGDQGAPGLDAPCPLGEDGLPVQGCWNK
*CL*PWIGLCVCLYIEYLFLYSFHFLKMPEV*C
ILQIIKKERKTCIFCTENINLFPFVYKMFCISLC
L*YIFCLVVMSA*YLCTFIKYRSLINYCVLVPK
GASQN*GAG*LMCEFT*M*RSMIFAKLGVSKSI
LNPYGFSLLKEMKHGN

Sequence NM_080804

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA
AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA
CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA
TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA
GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC
CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG
CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA
GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA
CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC
CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGA
AGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGTTCCTGGGCTGCCAGGGCCAGAGGGGCCTCCCGGACCTCCGGGGCTCCAAGGTGTTCCTGGACCAAAG
GGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTCCAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGTACTCCAGGACCAATTG
GAGTTCCAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGG
ACCACCCGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCC
CCCTGCCCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAAT
ATTTATTTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGA
AAATATCAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGC
ACATTTATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACAT
AAATGTAGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F4 (forward primer)
  • refseq_COL13A1.R4 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS*
KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD
CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA
CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR
RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA
LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE
QLENRDQMAPRAPRENQGKERWWITMETSMRLS
RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD
CQALQDTMGKRDLAVNQETWALLVPKAPQERMD
LQE*REKTGTQGAQERRGKKGRQDKQAHRERKE
KPGRRAIQEQRFLGCQGQRGLPDLRGSKVFLDQR
GKQD*MEQKERKASREKKETVVPWDYPVLQDQL
EFQAQRDQRARGAAKETLG*QDQREQLGFLVYM
DHPGTRETGGRGGRKALEGLKGIRETKERLD*MP
PAHWAKMAYQSKAAGTSDASNLGLACVCVCT*N
IYFYTVFTF*KCQKYDASYRLLKKKEKPAYFVQ
KISTSSLLFTRCFV*AYVSNTFFVWS*CLHDICA
HLLSIEA**IIVSWCQRGPARTEVLASSCVNSH
KCRGP*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEAAASPQFSATGSTPGHK
KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR
TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH
ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E
GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW
PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS
SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP
GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW
TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT
SRSEGRKRAPREPRREGGKRGDRTSRLTGRERR
SRGEGQSRSRGSWAARARGASRTSGAPRCSWTK
GGSRTRWSKRRERLPGRKRRPWSPGTTRYSRTNW
SSRPSGTKGREGQQRRPWDDRTNGSSWASWFTW
TTRGQGKPGGEGEERL*RA*RG*GRPRSAWIRC
PLPIGRRWLTSPRLLEQVMPLTLDWPVCVFVHRI
FIFIQFSLFENARSMMHLTDY*KRKKNLHILYR
KYQPLPFCLQDVLYKPMSLIHFLFGRNVCMIFV
HIY*VSKLNKLLCPGAKGGQPELRCWLAHV*IHI
NVEVHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK
RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG
LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG
MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK
GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG
PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG
AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ
EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG
LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG
PPGVKGENGHPGSPGEKGEKGETGQAGSPGEKGE
AGEKGNPGAEVPGLPGPEGPPGPPGLQGVPGPK
GEAGLDGAKGEKGFQGEKGDRGPLGLPGTPGPI
GVPGPAGPKGERGSKGDPGMTGPTGAAGLPGLHG
PPGDKGNRGERGKKGSRGPKGDKGDQGAPGLDA
PCPLGEDGLPVQGCWNK*CL*PWIGLCVCLYIE
YLFLYSFHFLKMPEV*CILQIIKKERKTCIFCTE
NINLFPFVYKMFCISLCL*YIFCLVVMSA*YLC
TFIKYRSLINYCVLVPKGASQN*GAG*LMCEFT
*M*RSMIFAKLGVSKSILNPYGFSLLKEMKHGN

Sequence NM_080803

AAGTAGCACGGATTGCTCATCCGATCCGTGCCGCCGCAGGGAGTGTGTCAAGTTACAGAGGCGCCGGAATCGGCCCCTGCGCTCCTCGCCAGCCGCCACG
ACCCACCTCTGCCCATGGGGCCCTCCGTGTGCGCCCCTTCGCCCGGGGACTGAAACTGACTGGCCCGGGAGACACGAGGCGCCCAGAAGGACTGACAGCG
CGGCACCAACTGCTCTGCAGACACTTGAAGGGAAAGACTGGGCGGAGAGAAGGAGAGCCGGTCAGATTCCCCTAACTTTCCTGGACTTGGAACGTTCTTC
GAAATAACTTTTTTCTCACCTAGGTGTACCCCAATTACCGCTGGTTGTGCTTTTTCGGCACTTCCTCTCCTACTGCTAATTTTTCCGTCCTCTTTGCCGG
GAGCAGCGGAAAGGGACGTTTTCCAGCGATACAAGCCCTTTCCCCCTGCCCCGCAGTTTGGATAGAGCCTTTTGGCAGCGGCTGTCGCCTTTATTTATTC
TATTTATTTATTTATTGGTTCTCAAGACGCGAGAGGATGGTAGCGGAGCGCACCCACAAAGCGGCAGCCACCGGTGCCCGCGGCCCTGGGGAGTTGGGCG
CGCCCGGGACGGTGGCTCTGGTGGCGGCGCGGGCGGAGCGCGGCGCACGGCTGCCGAGTCCAGGGTCGTGCGGGCTGCTGACGCTGGCCCTCTGCTCGCT
GGCACTCAGCCTGCTCGCCCACTTTCGGACGGCCGAGCTGCAGGCCCGGGTGCTGCGCCTGGAAGCGGAGCGCGGGGAGCAGCAAATGGAGACGGCTATT
TTGGGACGAGTCAATCAACTGCTGGACGAGAAATGGAAGCTCCACTCAAGGAGGCGCCGGGAGGCCCCAAAGACATCTCCAGGATGTAACTGCCCACCAG
CATTTCAGGGTCCCACTGGAAGACCCGGACTCCCAGGGGACAAAGGTGCCATTGGGATGCCTGGACGTGTGGGGTCCCCCGGAGACGCTGGGCTGTCCAT
CATTGGTCCCCGCGGCCCCCCTGGTCAACCAGGAACTAGAGGTTTCCCTGGATTTCCGGGTCCCATTGGGCTGGACGGCAAACCGGGCCACCCAGGACCA
AAGGGCGACATGGGTCTGACGGGTCCCCCAGGACAGCCGGGACCCCAGGGACAAAAAGGAGAAAAGGGTCAGTGTGGAGAGTACCCACACCGGGAGTGCC
TAAGCAGCATGCCAGCAGCTCTGCGCTCCAGCCAAATAATTGCCCTGAAGCTGCTGCCTCTCCTCAATTCAGTGCGACTGGCTCCACCCCCGGTCATAAA
AAGGCGGACGTTCCAGGGCGAACAGAGCCAGGCCAGCATCCAAGGTCCACCAGGGCCCCCAGGCCCCCCTGGACCAAGTGGACCTCTGGGGCACCCAGGA
CTGCCAGGGCCTATGGGGCCACCTGGCTTACCTGGGCCTCCTGGACCAAAGGGAGACCCAGGGATCCAGGGCTACCACGGCCGGAAGGGAGAACGGGGCA
TGCCAGGGATGCCAGGCAAGCATGGAGCCAAGGGGGCGCCCGGAATTGCCGTGGCTGGGATGAAGGGTGAGCCAGGGATCCCAGGAACCAAGGGTGAGAA
GGGGGCTGAAGGCTCCCCTGGGCTTCCTGGCCTCCTGGGGCAGAAGGGAGAGAAAGGCGATGCTGGCAACTCCATTGGAGGAGGCAGAGGGGAACCTGGC
CCTCCAGGGCTCCCTGGGCCCCCAGGGCCAAAGGGAGAAGCAGGTGTCGATGGCCAGGTTGGCCCCCCAGGGCAGCCAGGAGACAAGGGGGAGCGTGGAG
CAGCTGGAGAACAGGGACCAGATGGCCCCAAGGGCTCCAAGGGAGAACCAGGGAAAGGAGAGATGGTGGATTACAATGGAAACATCAATGAGGCTCTCCA
GGAGATCCGGACGCTGGCCTTGATGGGGCCTCCTGGTCTTCCTGGGCAAATTGGCCCACCTGGAGCTCCAGGGATTCCAGGCCAGAAGGGGGAGATTGGA
CTGCCAGGCCCTCCAGGACACGATGGGGAAAAGGGACCTCGCGGTAAACCAGGAGACATGGGCCCTCCTGGTCCCCAAGGCCCCCCAGGAAAGGATGGAC
CTCCAGGAGTGAAGGGAGAAAACGGGCACCCAGGGAGCCCAGGAGAGAAGGGGGAAAAAGGGGAGACAGGACAAGCAGGCTCACCGGGAGAGAAAGGAGA
AGCCGGGGAGAAGGGCAATCCAGGAGCAGAGGGGCTCCAAGGTGTTCCTGGACCAAAGGGGGAAGCAGGACTAGATGGAGCAAAAGGAGAGAAAGGCTTC
CAGGGAGAAAAAGGAGACCGTGGTCCCCTGGGACTACCCGGAGCTTCAGGTTTGGACGGCAGGCCTGGGCCACCGGGTACTCCAGGACCAATTGGAGTTC
CAGGCCCAGCGGGACCAAAGGGCGAGAGGGGCAGCAAAGGAGACCCTGGGATGACAGGACCAACGGGAGCAGCTGGGCTTCCTGGTTTACATGGACCACC
CGGGGACAAGGGAAACCGGGGGGAGAGGGGGAAGAAAGGCTCTAGAGGGCCTAAAGGGGATAAGGGAGACCAAGGAGCGCCTGGATTAGATGCCCCCTGC
CCATTGGGCGAAGATGGCTTACCAGTCCAAGGCTGCTGGAACAAGTGATGCCTCTAACCTTGGATTGGCCTGTGTGTGTGTTTGTACATAGAATATTTAT
TTTTATACAGTTTTCACTTTTTGAAAATGCCAGAAGTATGATGCATCTTACAGATTATTAAAAAAGAAAGAAAAACCTGCATATTTTGTACAGAAAATAT
CAACCTCTTCCCTTTTGTTTACAAGATGTTTTGTATAAGCCTATGTCTCTAATACATTTTTTGTTTGGTCGTAATGTCTGCATGATATTTGTGCACATTT
ATTAAGTATCGAAGCTTAATAAATTATTGTGTCCTGGTGCCAAAGGGGGCCAGCCAGAACTGAGGTGCTGGCTAGCTCATGTGTGAATTCACATAAATGT
AGAGGTCCATGATATTTGCTAAGCTAGGTGTGTCTAAGAGTATTTTAAACCCTTATGGATTTTCATTATTAAAGGAAATGAAACATGGCAATTC
  • refseq_COL13A1.F4 (forward primer)
  • refseq_COL13A1.R4 (reverse primer)
  • Single exon skipping

Frame 0

K*HGLLIRSVPPQGVCQVTEAPESAPALLASRH
DPPLPMGPSVCAPSPGD*N*LARETRGAQKD*QR
GTNCSADT*RERLGGEKESRSDSPNFPGLGTFF
EITFFSPRCTPITAGCAFSALPLLLLIFPSSLP
GAAERDVFQRYKPFPPAPQFG*SLLAAAVAFIYS
IYLFIGSQDARGW*RSAPTKRQPPVPAALGSWA
RPGRWLWWRRGRSAAHGCRVQGRAGC*RWPSAR
WHSACSPTFGRPSCRPGCCAWKRSAGSSKWRRLF
WDESINCWTRNGSSTQGGAGRPQRHLQDVTAHQ
HFRVPLEDPDSQGTKVPLGCLDVWGPPETLGCP
SLVPAAPLVNQELEVSLDFRVPLGWTANRATQDQ
RATWV*RVPQDSRDPRDKKEKRVSVESTHTGSA
*AACQQLCAPAK*LP*SCCLSSIQCDWLHPRS*
KGGRSRANRARPASKVHQGPQAPLDQVDLWGTQD
CQGLWGHLAYLGLLDQRETQGSRATTAGRENGA
CQGCQASMEPRGRPELPWLG*RVSQGSQEPRVR
RGLKAPLGFLASWGRRERKAMLATPLEEAEGNLA
LQGSLGPQGQREKQVSMARLAPQGSQETRGSVE
QLENRDQMAPRAPRENQGKERWWITMETSMRLS
RRSGRWP*WGLLVFLGKLAHLELQGFQARRGRLD
CQALQDTMGKRDLAVNQETWALLVPKAPQERMD
LQE*REKTGTQGAQERRGKKGRQDKQAHRERKE
KPGRRAIQEQRGSKVFLDQRGKQD*MEQKERKAS
REKKETVVPWDYPELQVWTAGLGHRVLQDQLEF
QAQRDQRARGAAKETLG*QDQREQLGFLVYMDH
PGTRETGGRGGRKALEGLKGIRETKERLD*MPPA
HWAKMAYQSKAAGTSDASNLGLACVCVCT*NIY
FYTVFTF*KCQKYDASYRLLKKKEKPAYFVQKI
STSSLLFTRCFV*AYVSNTFFVWS*CLHDICAHL
LSIEA**IIVSWCQRGPARTEVLASSCVNSHKC
RGP*YLLS*VCLRVF*TLMDFHY*RK*NMAI

Frame 1

SSTDCSSDPCRRRECVKLQRRRNRPLRSSPAAT
THLCPWGPPCAPLRPGTETDWPGRHEAPRRTDS
AAPTALQTLEGKDWAERRRAGQIPLTFLDLERSS
K*LFSHLGVPQLPLVVLFRHFLSYC*FFRPLCR
EQRKGTFSSDTSPFPLPRSLDRAFWQRLSPLFI
LFIYLLVLKTREDGSGAHPQSGSHRCPRPWGVGR
ARDGGSGGGAGGARRTAAESRVVRAADAGPLLA
GTQPARPLSDGRAAGPGAAPGSGARGAANGDGY
FGTSQSTAGREMEAPLKEAPGGPKDISRM*LPTS
ISGSHWKTRTPRGQRCHWDAWTCGVPRRRWAVH
HWSPRPPWSTRN*RFPWISGSHWAGRQTGPPRT
KGRHGSDGSPRTAGTPGTKRRKGSVWRVPTPGVP
KQHASSSALQPNNCPEAAASPQFSATGSTPGHK
KADVPGRTEPGQHPRSTRAPRPPWTKWTSGAPR
TARAYGATWLTWASWTKGRPRDPGLPRPEGRTGH
ARDARQAWSQGGARNCRGWDEG*ARDPRNQG*E
GG*RLPWASWPPGAEGRERRCWQLHWRRQRGTW
PSRAPWAPRAKGRSRCRWPGWPPRAARRQGGAWS
SWRTGTRWPQGLQGRTRERRDGGLQWKHQ*GSP
GDPDAGLDGASWSSWANWPTWSSRDSRPEGGDW
TARPSRTRWGKGTSR*TRRHGPSWSPRPPRKGWT
SRSEGRKRAPREPRREGGKRGDRTSRLTGRERR
SRGEGQSRSRGAPRCSWTKGGSRTRWSKRRERL
PGRKRRPWSPGTTRSFRFGRQAWATGYSRTNWSS
RPSGTKGREGQQRRPWDDRTNGSSWASWFTWTT
RGQGKPGGEGEERL*RA*RG*GRPRSAWIRCPL
PIGRRWLTSPRLLEQVMPLTLDWPVCVFVHRIFI
FIQFSLFENARSMMHLTDY*KRKKNLHILYRKY
QPLPFCLQDVLYKPMSLIHFLFGRNVCMIFVHI
Y*VSKLNKLLCPGAKGGQPELRCWLAHV*IHINV
EVHDIC*ARCV*EYFKPLWIFIIKGNETWQF

Frame 2

VARIAHPIRAAAGSVSSYRGAGIGPCAPRQPPR
PTSAHGALRVRPFARGLKLTGPGDTRRPEGLTA
RHQLLCRHLKGKTGRREGEPVRFP*LSWTWNVL
RNNFFLT*VYPNYRWLCFFGTSSPTANFSVLFAG
SSGKGRFPAIQALSPCPAVWIEPFGSGCRLYLF
YLFIYWFSRRERMVAERTHKAAATGARGPGELG
APGTVALVAARAERGARLPSPGSCGLLTLALCSL
ALSLLAHFRTAELQARVLRLEAERGEQQMETAI
LGRVNQLLDEKWKLHSRRRREAPKTSPGCNCPP
AFQGPTGRPGLPGDKGAIGMPGRVGSPGDAGLSI
IGPRGPPGQPGTRGFPGFPGPIGLDGKPGHPGP
KGDMGLTGPPGQPGPQGQKGEKGQCGEYPHREC
LSSMPAALRSSQIIALKLLPLLNSVRLAPPPVIK
RRTFQGEQSQASIQGPPGPPGPPGPSGPLGHPG
LPGPMGPPGLPGPPGPKGDPGIQGYHGRKGERG
MPGMPGKHGAKGAPGIAVAGMKGEPGIPGTKGEK
GAEGSPGLPGLLGQKGEKGDAGNSIGGGRGEPG
PPGLPGPPGPKGEAGVDGQVGPPGQPGDKGERG
AAGEQGPDGPKGSKGEPGKGEMVDYNGNINEALQ
EIRTLALMGPPGLPGQIGPPGAPGIPGQKGEIG
LPGPPGHDGEKGPRGKPGDMGPPGPQGPPGKDG
PPGVKGENGHPGSPGEKGEKGETGQAGSPGEKGE
AGEKGNPGAEGLQGVPGPKGEAGLDGAKGEKGF
QGEKGDRGPLGLPGASGLDGRPGPPGTPGPIGV
PGPAGPKGERGSKGDPGMTGPTGAAGLPGLHGPP
GDKGNRGERGKKGSRGPKGDKGDQGAPGLDAPC
PLGEDGLPVQGCWNK*CL*PWIGLCVCLYIEYL
FLYSFHFLKMPEV*CILQIIKKERKTCIFCTENI
NLFPFVYKMFCISLCL*YIFCLVVMSA*YLCTF
IKYRSLINYCVLVPKGASQN*GAG*LMCEFT*M
*RSMIFAKLGVSKSILNPYGFSLLKEMKHGN

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