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Sequences

Sequence NM_001025072

ACCTCCGGTCGCTGGACGTGCTCTCGCGTAGCTCCGCCTTTCGTAAGTCCCCCCGCCTCGCATGATGGCTGCGGTGCCGCCGGGCCTGGAGCCGTGGAAC
CGTGTGAGAATCCCTAAGGCGGGGAACCGCAGCGCAGTGACAGTGCAGAACCCCGGCGCGGCCCTTGGTTGAGCAATGTTTAAAGCGTTTGAAAAACATG
AATTTGGAGGGCTCAATTCAAGACCTGTTTGAGTTGTTTTCTTCCAAGTAATGAAAATCAGCCCTTAACTACCAAAGTATGTGTTGTCCCCAGTCAGCCA
GTGGTGGAGTTGGTGTTGATGAAGGTTTTGGGAGCCTGCAAGTTGTTGCTCCGCTTGTTGGACTGCTGCTGCAAAACTTTTCTTTTGACTGTGAAACATC
TAGGTTTGCAAGAGTTCATTATTTTAAACCTTGTGATGGTTGGGCTGGTGAGCAGGTTATGGGTTCTCTATAAAGGTGTCTTAAAAAGGTTGATTTTGTT
ATATGAGCCTTTGTTTGGATTGCTTCAAGAGGTCGCTAGGATTCAACCAATGCCTTACTTCAAAGATTTTACCTTTCCTTCTGATATCACTGAATTTTTA
GGACAGCCATATTTTGAAGCCTTTAAGAAAAAAATGCCTATAGCTTTTGCAGCTAAAGGAATAAATAAATTGCTAAATAAACTGTTTTTAATAAATGAGC
AGTCACCAAGAGCCAGTGAAGAAACCTTGCTTGGAATTTCAAAAAAAGCTAAACAAATGAAGATCAATGTACAGAATAATGTGGATCTTGGACAGCCAGT
AAAGAATAAGAGAGTCTTCAAAGAAGAGTCATCAGAATTTGATGTGAGGGCTTTCTGCAACCAGCTGAAACACAAAGCTACTCAGGAGACCAGTTTTGAT
TTTAAATGTTCTCAATCCAGACTAAAGACAACCAAGTATTCTTCTCAGAAAGTGATAGGAACTCCTCATGCCAAAAGTTTTGTGCAAAGATTCCGAGAGG
CTGAGTCCTTCACACAACTTTCTGAAGAAATCCAGATGGCAGTTGTATGGTGCAGGAGCAAAAAACTCAAGGCTCAGGCCATTTTTCTGGGTAACAAACT
TCTTAAAAGCAACCGGCTTAAACATCTGGAAGCTCAAGGTACTAGTTTGCCAAAGAAACTAGAGTGCATAAAAACGTCTATTTGCAACCACCTTCTTCGT
GGCTCAGGTATCAAAACTTCAAAGCATCATCTGAGACAGAGAAGATCACAGAATAAATTTTTACGGAGACAAAGGAAACCACAGAGAAAGTTGCAGTCGA
CTCTTTTAAGGGAAATTCAGCAGTTCTCTCAAGGGACTCGGAAGAGTGCTACAGATACCAGTGCTAAGTGGAGACTCTCACACTGTACTGTGCATAGAAC
TGATCTCTACCCTAACAGTAAGCAGCTCTTGAATAGTGGAGTTTCAATGCCTGTCATACAAACTAAGGAGAAAATGATTCATGAAAATCTTAGAGGCATC
CATGAAAATGAAACTGATTCGTGGACGGTGATGCAAATAAATAAAAACAGTACATCAGGAACCATTAAGGAGACAGATGACATTGATGATATTTTTGCTT
TAATGGGAGTTTAGATGTTCGTTCATATGTGAGACTTTTAAGTGATTAACAAGGCTAGTTCAGTGTTCTAAGTAGAACTGCTAAGATCTGGAAGTACCAC
CTGGACTCACAGAGGAGCTGCTTTAGTGCAGCATTGCACAGGAGTTCATTTCAGTTCAGTCTACATTTAAAGTAGCATTGTGTCATTTCATCAACAAGTA
TTTCTTTTTTGGCTTCAACTACATGTCAGGTGTTAATATGAAAAATCTGCCACAGCCTACCCTTGAGAAGCAGATGTAATTCCTTGAGTGAGGCTAGTAA
GTCTCTGAAAAACTGAATAATTTTATGACTTACTAGGCAATTTAATAATCGTTTGCAAAAACCATTGTGTCTTGAGGTAAATGAGGAGCGGGGGTGGGAG
ATAGCAAGGAAACACCTGAAAGGTCAATTTGGTCTTATTGGCTCCATTATACATAGGTCCGCCTCTGAATACTAAAGTTTGTAGCTCCTGACATTTATGT
TTTCTTGTTTATATGTTGGAATTGATCAACAAGGTTTGCCACTGCTTGTATTACCAGGGACTGGTTACAACCATTATTTCTCTTCATTTGCTTGGCTTAT
CTCATATTAAAGTGAGTTTGGAGTTCTCCAAAACGTTTTAATTTTATTTATTTGGGTACCAGTGATTGATTCCAAATAAACCTTTTTACAAAAAGGCAAT
AAGGTGGTTTATGGTTACATATGAAATATAATAGGATAACAGGTAGATGAGAAAGTAAAATGAAAAAATGAGGTTAGGAGATACAAAGTAGACCCAGGAA
TGAGGCTAGAACATTTATAATTTGGTGTTGATGCTGTCATCTGATAGGGGATGTTGCTCGTACTGTTGCTCTGAGGGGAGTACATCTTGAGTATTTTCAA
ATAATGATGTGAAGTTTGAGGTAGGTATCTTTCCAATGTTCTAACTTTACTAGAATTAGGTTATTAAGCTTCTCTAGTGCCCTTAACTTTTGTTACATAG
AGATTTAAAGAAAAAGAAAGTAGTACCTTAGTATGTACCGCATCCTTCTTTTCCTCAGCCACCCTATCATAACAGAAGTTATTTCCCTTCCATTCTTTAT
TTGGCTTTGTTCTTTCCCTGTCTGCCTTCTCCATTTTCTCAGTTATCTCTACCAGTGGTTCTCAAACTTTAGACTTTTTGAGAATCACCTGGTGGGTTGC
TAAAAGATGCAGATTAGCCCTTTCCCAGAGACTGATAAACAGGGGCCAGAAACCTGCATTTTTAACATGGAGCCCAAGTGATTTTGATCCAGGTGGCCCC
CAAATCCAGTTATATAAACACTACTTTAGCTTCTCTCCTGAAGCCTTAAGTAAACTGGTTTAATTCTTAATCTGTAGTCAGTTTTCATATTCCTTTTGGC
TTTCTACCAAGTGGTAGTATCCATTTAGTGCCTTAAAATACAGACAATACCAAGGAGATTTCATTCTAGACAAGTCTAGAAATGGCCATTAACTTCTAAG
TGTCCTTGTGTTCAATGAATATCCTTTACCTATTGGGAAATAGAAGGTAAATTAGTAAGAGAACTGGTGTATAGCTTTGTGGACCATAGGGCTTATTTGC
TGAGAGTTGTCTATATTCATGCACCCAGTCCCCTAAGTAGCCTCTTTTAATACTTCATTTAGCTGATTCAGAAAAAAACTCCCTGGGAATTAGGAGATTT
AAGGTTCAGTCTTGCTTCTCCTACTTGTGGCTTCACTGAGCTAGTCAGTTTCCTCAGGCTTAGGTTTCTGGATTTGAAGTGGAATTTTTTCAAGAATGAA
ACTTTTATAGCTGAAATAAACTTTGGAGCTTATCTTTCATTGGAAAAACCAGGGTGGTTCAGTGAGTTAAGGACAATATAGCATGGACTGGGCTGCCAGT
CTAGCATTTGTTTCCAAATTCATTCATGTTCTTTTGGAGTATCTTCATCTCCTCTCATTTTCCCATCTTCTGCCCTTTACTATTCTGGGAAAATGGCAAA
AAAGCTTCACATGTGTCCACAAAATGACAATTTGTATGTTTATAAGGAATTCAGTATTCTGTATCAGTTACTTTTTCCAAATAAATCCAAATATTTAACA
TTTAAAAAAATGCAATCTAAAGTATACCAAACTGTAACTTACCTAGTAAATGAGATTAGAAACTATTGTCAGGGTCATCGTAGTGAATTATTATTATTAT
TATTTTTTGAGACAGAGTCTTGTGCTGTTGCCCAGGCTGGAGCACAGTGGTATGATCTCAGTTCACTGCACCCTCCACCTCCTGGGCTCAAGCTATTCTC
CCACCTCAGCCGCCTGAGTAGCTGACACTATAGGCACCACCATGCCCTGGCTAATTTTTTAAAAACTTTGTGTAGAGATAGGGTTTCACCATGTTGCTTA
GGCTGGTCTCAAACTCCTGGACTCAAGGGATCCACCCACTTTGGTCTCCCAAAGTGCTGGGATTACCCACATCCTAGTACCAACTTTATCACTTCTGATG
GCATTAGGACACACAGGCCATTTATAAGAGCAGTGTTTGAGACCTCCTGTGTGTCACTTCCAATCCTCTTGGCCTTCATACTAAAGTCACTGCTGTGGCA
ACCAGTTTCAAGCAGGGCTGAACCTTGCCTCTTGCTCTGGGGATGTCTCACTTCCTGCCCCTGGGCTTCTCACCAGCTACAGGAACTGCCTAGCACTTGG
GTATGTACAACCTGGAAGAGGTGTGGGGCCACCCCATCCCCTCCTCAAAGAAATTCACAGGCACAGTATGAGGCTAAAGTAACTTCCATCTTGGATGCTA
ATCTACCATGTTGACTTCTGATAACCAGTTCCTGGAATGCCCTCTAAGATTTCTACTTTTATCTATTAAAAGAACACACACACACACACACACTGTAAAT
CCTGGCCTTAAGCAAGTTATAGGCTAAGTTGTAAACAGCATTCTTGCCTTTCCCTGAGGAGGTGACTTCAGTGTCTCACATGTTCCTTCATGTACCCCCT
TTTCTCTATGGCATATAAGCCCTGGGTCGGGGGGTAACTGTGGGGATCACTGCCTGAGACATGGCTTCTGTGCATAAGTCCCTATTAAATGTTTCTTTCT
GACAAA
  • refseq_C3orf17.F1 (forward primer)
  • refseq_C3orf17.R1 (reverse primer)
  • Single exon skipping

Frame 0

TSGRWTCSRVAPPFVSPPASHDGCGAAGPGAVE
PCENP*GGEPQRSDSAEPRRGPWLSNV*SV*KT*
IWRAQFKTCLSCFLPSNENQPLTTKVCVVPSQP
VVELVLMKVLGACKLLLRLLDCCCKTFLLTVKH
LGLQEFIILNLVMVGLVSRLWVLYKGVLKRLILL
YEPLFGLLQEVARIQPMPYFKDFTFPSDITEFL
GQPYFEAFKKKMPIAFAAKGINKLLNKLFLINE
QSPRASEETLLGISKKAKQMKINVQNNVDLGQPV
KNKRVFKEESSEFDVRAFCNQLKHKATQETSFD
FKCSQSRLKTTKYSSQKVIGTPHAKSFVQRFRE
AESFTQLSEEIQMAVVWCRSKKLKAQAIFLGNKL
LKSNRLKHLEAQGTSLPKKLECIKTSICNHLLR
GSGIKTSKHHLRQRRSQNKFLRRQRKPQRKLQS
TLLREIQQFSQGTRKSATDTSAKWRLSHCTVHRT
DLYPNSKQLLNSGVSMPVIQTKEKMIHENLRGI
HENETDSWTVMQINKNSTSGTIKETDDIDDIFA
LMGV*MFVHM*DF*VINKASSVF*VELLRSGSTT
WTHRGAALVQHCTGVHFSSVYI*SSIVSFHQQV
FLFWLQLHVRC*YEKSATAYP*EADVIP*VRLV
SL*KTE*FYDLLGNLIIVCKNHCVLR*MRSGGGR
*QGNT*KVNLVLLAPLYIGPPLNTKVCSS*HLC
FLVYMLELINKVCHCLYYQGLVTTIISLHLLGL
SHIKVSLEFSKTF*FYLFGYQ*LIPNKPFYKKAI
RWFMVTYEI**DNR*MRK*NEKMRLGDTK*TQE
*G*NIYNLVLMLSSDRGCCSYCCSEGSTS*VFS
NNDVKFEVGIFPMF*LY*N*VIKLL*CP*LLLHR
DLKKKKVVP*YVPHPSFPQPPYHNRSYFPSILY
LALFFPCLPSPFSQLSLPVVLKL*TF*ESPGGL
LKDAD*PFPRD**TGARNLHF*HGAQVILIQVAP
KSSYINTTLASLLKP*VNWFNS*SVVSFHIPFG
FLPSGSIHLVP*NTDNTKEISF*TSLEMAINF*
VSLCSMNILYLLGNRR*ISKRTGV*LCGP*GLFA
ESCLYSCTQSPK*PLLILHLADSEKNSLGIRRF
KVQSCFSYLWLH*ASQFPQA*VSGFEVEFFQE*
NFYS*NKLWSLSFIGKTRVVQ*VKDNIAWTGLPV
*HLFPNSFMFFWSIFISSHFPIFCPLLFWENGK
KASHVSTK*QFVCL*GIQYSVSVTFSK*IQIFN
I*KNAI*SIPNCNLPSK*D*KLLSGSS**IIIII
IF*DRVLCCCPGWSTVV*SQFTAPSTSWAQAIL
PPQPPE*LTL*APPCPG*FFKNFV*R*GFTMLL
RLVSNSWTQGIHPLWSPKVLGLPTS*YQLYHF*W
H*DTQAIYKSSV*DLLCVTSNPLGLHTKVTAVA
TSFKQG*TLPLALGMSHFLPLGFSPATGTA*HL
GMYNLEEVWGHPIPSSKKFTGTV*G*SNFHLGC*
STMLTSDNQFLECPLRFLLLSIKRTHTHTHTVN
PGLKQVIG*VVNSILAFP*GGDFSVSHVPSCTP
FSLWHISPGSGGNCGDHCLRHGFCA*VPIKCFFL
T

Frame 1

PPVAGRALA*LRLS*VPPPRMMAAVPPGLEPWN
RVRIPKAGNRSAVTVQNPGAALG*AMFKAFEKH
EFGGLNSRPV*VVFFQVMKISP*LPKYVLSPVSQ
WWSWC**RFWEPASCCSACWTAAAKLFF*L*NI
*VCKSSLF*TL*WLGW*AGYGFSIKVS*KG*FC
YMSLCLDCFKRSLGFNQCLTSKILPFLLISLNF*
DSHILKPLRKKCL*LLQLKE*INC*INCF**MS
SHQEPVKKPCLEFQKKLNK*RSMYRIMWILDSQ
*RIRESSKKSHQNLM*GLSATS*NTKLLRRPVLI
LNVLNPD*RQPSILLRK**ELLMPKVLCKDSER
LSPSHNFLKKSRWQLYGAGAKNSRLRPFFWVTN
FLKATGLNIWKLKVLVCQRN*SA*KRLFATTFFV
AQVSKLQSII*DREDHRINFYGDKGNHRESCSR
LF*GKFSSSLKGLGRVLQIPVLSGDSHTVLCIE
LISTLTVSSS*IVEFQCLSYKLRRK*FMKILEAS
MKMKLIRGR*CK*IKTVHQEPLRRQMTLMIFLL
*WEFRCSFICETFK*LTRLVQCSK*NC*DLEVP
PGLTEELL*CSIAQEFISVQSTFKVALCHFINKY
FFFGFNYMSGVNMKNLPQPTLEKQM*FLE*G**
VSEKLNNFMTY*AI**SFAKTIVS*GK*GAGVG
DSKETPERSIWSYWLHYT*VRL*ILKFVAPDIYV
FLFICWN*STRFATACITRDWLQPLFLFICLAY
LILK*VWSSPKRFNFIYLGTSD*FQINLFTKRQ
*GGLWLHMKYNRITGR*ESKMKK*G*EIQSRPRN
EARTFIIWC*CCHLIGDVARTVALRGVHLEYFQ
IMM*SLR*VSFQCSNFTRIRLLSFSSALNFCYI
EI*RKRK*YLSMYRILLFLSHPIITEVISLPFFI
WLCSFPVCLLHFLSYLYQWFSNFRLFENHLVGC
*KMQISPFPETDKQGPETCIFNMEPK*F*SRWP
PNPVI*TLL*LLS*SLK*TGLILNL*SVFIFLLA
FYQVVVSI*CLKIQTIPRRFHSRQV*KWPLTSK
CPCVQ*ISFTYWEIEGKLVRELVYSFVDHRAYL
LRVVYIHAPSPLSSLF*YFI*LIQKKTPWELGDL
RFSLASPTCGFTELVSFLRLRFLDLKWNFFKNE
TFIAEINFGAYLSLEKPGWFSELRTI*HGLGCQ
SSICFQIHSCSFGVSSSPLIFPSSALYYSGKMAK
KLHMCPQNDNLYVYKEFSILYQLLFPNKSKYLT
FKKMQSKVYQTVTYLVNEIRNYCQGHRSELLLL
LFFETESCAVAQAGAQWYDLSSLHPPPPGLKLFS
HLSRLSS*HYRHHHALANFLKTLCRDRVSPCCL
GWSQTPGLKGSTHFGLPKCWDYPHPSTNFITSD
GIRTHRPFIRAVFETSCVSLPILLAFILKSLLWQ
PVSSRAEPCLLLWGCLTSCPWASHQLQELPSTW
VCTTWKRCGATPSPPQRNSQAQYEAKVTSILDA
NLPC*LLITSSWNAL*DFYFYLLKEHTHTHTL*I
LALSKL*AKL*TAFLPFPEEVTSVSHMFLHVPP
FLYGI*ALGRGVTVGITA*DMASVHKSLLNVSF
*Q

Frame 2

LRSLDVLSRSSAFRKSPRLA*WLRCRRAWSRGT
V*ESLRRGTAAQ*QCRTPARPLVEQCLKRLKNM
NLEGSIQDLFELFSSK**KSALNYQSMCCPQSA
SGGVGVDEGFGSLQVVAPLVGLLLQNFSFDCETS
RFARVHYFKPCDGWAGEQVMGSL*RCLKKVDFV
I*AFVWIASRGR*DSTNALLQRFYLSF*YH*IF
RTAIF*SL*EKNAYSFCS*RNK*IAK*TVFNK*A
VTKSQ*RNLAWNFKKS*TNEDQCTE*CGSWTAS
KE*ESLQRRVIRI*CEGFLQPAETQSYSGDQF*
F*MFSIQTKDNQVFFSESDRNSSCQKFCAKIPRG
*VLHTTF*RNPDGSCMVQEQKTQGSGHFSG*QT
S*KQPA*TSGSSRY*FAKETRVHKNVYLQPPSS
WLRYQNFKASSETEKITE*IFTETKETTEKVAVD
SFKGNSAVLSRDSEECYRYQC*VETLTLYCA*N
*SLP*Q*AALE*WSFNACHTN*GENDS*KS*RH
P*K*N*FVDGDANK*KQYIRNH*GDR*H**YFCF
NGSLDVRSYVRLLSD*QG*FSVLSRTAKIWKYH
LDSQRSCFSAALHRSSFQFSLHLK*HCVISSTS
ISFLASTTCQVLI*KICHSLPLRSRCNSLSEASK
SLKN*IIL*LTRQFNNRLQKPLCLEVNEERGWE
IARKHLKGQFGLIGSIIHRSASEY*SL*LLTFM
FSCLYVGIDQQGLPLLVLPGTGYNHYFSSFAWLI
SY*SEFGVLQNVLILFIWVPVIDSK*TFLQKGN
KVVYGYI*NIIG*QVDEKVK*KNEVRRYKVDPG
MRLEHL*FGVDAVI**GMLLVLLL*GEYILSIFK
**CEV*GRYLSNVLTLLELGY*ASLVPLTFVT*
RFKEKESSTLVCTASFFSSATLS*QKLFPFHSL
FGFVLSLSAFSIFSVISTSGSQTLDFLRITWWVA
KRCRLALSQRLINRGQKPAFLTWSPSDFDPGGP
QIQLYKHYFSFSPEALSKLV*FLICSQFSYSFW
LSTKW*YPFSALKYRQYQGDFILDKSRNGH*LLS
VLVFNEYPLPIGK*KVN**ENWCIALWTIGLIC
*ELSIFMHPVP*VASFNTSFS*FRKKLPGN*EI
*GSVLLLLLVASLS*SVSSGLGFWI*SGIFSRMK
LL*LK*TLELIFHWKNQGGSVS*GQYSMDWAAS
LAFVSKFIHVLLEYLHLLSFSHLLPFTILGKWQ
KSFTCVHKMTICMFIRNSVFCISYFFQINPNI*H
LKKCNLKYTKL*LT**MRLETIVRVIVVNYYYY
YFLRQSLVLLPRLEHSGMISVHCTLHLLGSSYS
PTSAA*VADTIGTTMPWLIF*KLCVEIGFHHVA*
AGLKLLDSRDPPTLVSQSAGITHILVPTLSLLM
ALGHTGHL*EQCLRPPVCHFQSSWPSY*SHCCG
NQFQAGLNLASCSGDVSLPAPGLLTSYRNCLALG
YVQPGRGVGPPHPLLKEIHRHSMRLK*LPSWML
IYHVDF**PVPGMPSKISTFIY*KNTHTHTHCK
SWP*ASYRLSCKQHSCLSLRR*LQCLTCSFMYPL
FSMAYKPWVGG*LWGSLPETWLLCISPY*MFLS
DK

Sequence NM_001025073

ACCTCCGGTCGCTGGACGTGCTCTCGCGTAGCTCCGCCTTTCGTAAGTCCCCCCGCCTCGCATGATGGCTGCGGTGCCGCCGGGCCTGGAGCCGTGGAAC
CGTGTGAGAATCCCTAAGGCGGGGAACCGCAGCGCAGTGACAGTGCAGAACCCCGGCGCGGCCCTTGGTTGAGCAATGTTTAAAGCGTTTGAAAAACATG
AATTTGGAGGGCTCAATTCAAGACCTGTTTGAGTTGTTTTCTTCCAATGAAAATCAGCCCTTAACTACCAAAGTATGTGTTGTCCCCAGTCAGCCAGTGG
TGGAGTTGGTGTTGATGAAGGTTTTGGGAGCCTGCAAGTTGTTGCTCCGCTTGTTGGACTGCTGCTGCAAAACTTTTCTTTTGACTGTGAAACATCTAGG
TTTGCAAGAGTTCATTATTTTAAACCTTGTGATGGTTGGGCTGGTGAGCAGGTTATGGGTTCTCTATAAAGGTGTCTTAAAAAGGTTGATTTTGTTATAT
GAGCCTTTGTTTGGATTGCTTCAAGAGGTCGCTAGGATTCAACCAATGCCTTACTTCAAAGATTTTACCTTTCCTTCTGATATCACTGAATTTTTAGGAC
AGCCATATTTTGAAGCCTTTAAGAAAAAAATGCCTATAGCTTTTGCAGCTAAAGGAATAAATAAATTGCTAAATAAACTGTTTTTAATAAATGAGCAGTC
ACCAAGAGCCAGTGAAGAAACCTTGCTTGGAATTTCAAAAAAAGCTAAACAAATGAAGATCAATGTACAGAATAATGTGGATCTTGGACAGCCAGTAAAG
AATAAGAGAGTCTTCAAAGAAGAGTCATCAGAATTTGATGTGAGGGCTTTCTGCAACCAGCTGAAACACAAAGCTACTCAGGAGACCAGTTTTGATTTTA
AATGTTCTCAATCCAGACTAAAGACAACCAAGTATTCTTCTCAGAAAGTGATAGGAACTCCTCATGCCAAAAGTTTTGTGCAAAGATTCCGAGAGGCTGA
GTCCTTCACACAACTTTCTGAAGAAATCCAGATGGCAGTTGTATGGTGCAGGAGCAAAAAACTCAAGGCTCAGGCCATTTTTCTGGGTAACAAACTTCTT
AAAAGCAACCGGCTTAAACATCTGGAAGCTCAAGGTACTAGTTTGCCAAAGAAACTAGAGTGCATAAAAACGTCTATTTGCAACCACCTTCTTCGTGGCT
CAGGTATCAAAACTTCAAAGCATCATCTGAGACAGAGAAGATCACAGAATAAATTTTTACGGAGACAAAGGAAACCACAGAGAAAGTTGCAGTCGACTCT
TTTAAGGGAAATTCAGCAGTTCTCTCAAGGGACTCGGAAGAGTGCTACAGATACCAGTGCTAAGTGGAGACTCTCACACTGTACTGTGCATAGAACTGAT
CTCTACCCTAACAGTAAGCAGCTCTTGAATAGTGGAGTTTCAATGCCTGTCATACAAACTAAGGAGAAAATGATTCATGAAAATCTTAGAGGCATCCATG
AAAATGAAACTGATTCGTGGACGGTGATGCAAATAAATAAAAACAGTACATCAGGAACCATTAAGGAGACAGATGACATTGATGATATTTTTGCTTTAAT
GGGAGTTTAGATGTTCGTTCATATGTGAGACTTTTAAGTGATTAACAAGGCTAGTTCAGTGTTCTAAGTAGAACTGCTAAGATCTGGAAGTACCACCTGG
ACTCACAGAGGAGCTGCTTTAGTGCAGCATTGCACAGGAGTTCATTTCAGTTCAGTCTACATTTAAAGTAGCATTGTGTCATTTCATCAACAAGTATTTC
TTTTTTGGCTTCAACTACATGTCAGGTGTTAATATGAAAAATCTGCCACAGCCTACCCTTGAGAAGCAGATGTAATTCCTTGAGTGAGGCTAGTAAGTCT
CTGAAAAACTGAATAATTTTATGACTTACTAGGCAATTTAATAATCGTTTGCAAAAACCATTGTGTCTTGAGGTAAATGAGGAGCGGGGGTGGGAGATAG
CAAGGAAACACCTGAAAGGTCAATTTGGTCTTATTGGCTCCATTATACATAGGTCCGCCTCTGAATACTAAAGTTTGTAGCTCCTGACATTTATGTTTTC
TTGTTTATATGTTGGAATTGATCAACAAGGTTTGCCACTGCTTGTATTACCAGGGACTGGTTACAACCATTATTTCTCTTCATTTGCTTGGCTTATCTCA
TATTAAAGTGAGTTTGGAGTTCTCCAAAACGTTTTAATTTTATTTATTTGGGTACCAGTGATTGATTCCAAATAAACCTTTTTACAAAAAGGCAATAAGG
TGGTTTATGGTTACATATGAAATATAATAGGATAACAGGTAGATGAGAAAGTAAAATGAAAAAATGAGGTTAGGAGATACAAAGTAGACCCAGGAATGAG
GCTAGAACATTTATAATTTGGTGTTGATGCTGTCATCTGATAGGGGATGTTGCTCGTACTGTTGCTCTGAGGGGAGTACATCTTGAGTATTTTCAAATAA
TGATGTGAAGTTTGAGGTAGGTATCTTTCCAATGTTCTAACTTTACTAGAATTAGGTTATTAAGCTTCTCTAGTGCCCTTAACTTTTGTTACATAGAGAT
TTAAAGAAAAAGAAAGTAGTACCTTAGTATGTACCGCATCCTTCTTTTCCTCAGCCACCCTATCATAACAGAAGTTATTTCCCTTCCATTCTTTATTTGG
CTTTGTTCTTTCCCTGTCTGCCTTCTCCATTTTCTCAGTTATCTCTACCAGTGGTTCTCAAACTTTAGACTTTTTGAGAATCACCTGGTGGGTTGCTAAA
AGATGCAGATTAGCCCTTTCCCAGAGACTGATAAACAGGGGCCAGAAACCTGCATTTTTAACATGGAGCCCAAGTGATTTTGATCCAGGTGGCCCCCAAA
TCCAGTTATATAAACACTACTTTAGCTTCTCTCCTGAAGCCTTAAGTAAACTGGTTTAATTCTTAATCTGTAGTCAGTTTTCATATTCCTTTTGGCTTTC
TACCAAGTGGTAGTATCCATTTAGTGCCTTAAAATACAGACAATACCAAGGAGATTTCATTCTAGACAAGTCTAGAAATGGCCATTAACTTCTAAGTGTC
CTTGTGTTCAATGAATATCCTTTACCTATTGGGAAATAGAAGGTAAATTAGTAAGAGAACTGGTGTATAGCTTTGTGGACCATAGGGCTTATTTGCTGAG
AGTTGTCTATATTCATGCACCCAGTCCCCTAAGTAGCCTCTTTTAATACTTCATTTAGCTGATTCAGAAAAAAACTCCCTGGGAATTAGGAGATTTAAGG
TTCAGTCTTGCTTCTCCTACTTGTGGCTTCACTGAGCTAGTCAGTTTCCTCAGGCTTAGGTTTCTGGATTTGAAGTGGAATTTTTTCAAGAATGAAACTT
TTATAGCTGAAATAAACTTTGGAGCTTATCTTTCATTGGAAAAACCAGGGTGGTTCAGTGAGTTAAGGACAATATAGCATGGACTGGGCTGCCAGTCTAG
CATTTGTTTCCAAATTCATTCATGTTCTTTTGGAGTATCTTCATCTCCTCTCATTTTCCCATCTTCTGCCCTTTACTATTCTGGGAAAATGGCAAAAAAG
CTTCACATGTGTCCACAAAATGACAATTTGTATGTTTATAAGGAATTCAGTATTCTGTATCAGTTACTTTTTCCAAATAAATCCAAATATTTAACATTTA
AAAAAATGCAATCTAAAGTATACCAAACTGTAACTTACCTAGTAAATGAGATTAGAAACTATTGTCAGGGTCATCGTAGTGAATTATTATTATTATTATT
TTTTGAGACAGAGTCTTGTGCTGTTGCCCAGGCTGGAGCACAGTGGTATGATCTCAGTTCACTGCACCCTCCACCTCCTGGGCTCAAGCTATTCTCCCAC
CTCAGCCGCCTGAGTAGCTGACACTATAGGCACCACCATGCCCTGGCTAATTTTTTAAAAACTTTGTGTAGAGATAGGGTTTCACCATGTTGCTTAGGCT
GGTCTCAAACTCCTGGACTCAAGGGATCCACCCACTTTGGTCTCCCAAAGTGCTGGGATTACCCACATCCTAGTACCAACTTTATCACTTCTGATGGCAT
TAGGACACACAGGCCATTTATAAGAGCAGTGTTTGAGACCTCCTGTGTGTCACTTCCAATCCTCTTGGCCTTCATACTAAAGTCACTGCTGTGGCAACCA
GTTTCAAGCAGGGCTGAACCTTGCCTCTTGCTCTGGGGATGTCTCACTTCCTGCCCCTGGGCTTCTCACCAGCTACAGGAACTGCCTAGCACTTGGGTAT
GTACAACCTGGAAGAGGTGTGGGGCCACCCCATCCCCTCCTCAAAGAAATTCACAGGCACAGTATGAGGCTAAAGTAACTTCCATCTTGGATGCTAATCT
ACCATGTTGACTTCTGATAACCAGTTCCTGGAATGCCCTCTAAGATTTCTACTTTTATCTATTAAAAGAACACACACACACACACACACTGTAAATCCTG
GCCTTAAGCAAGTTATAGGCTAAGTTGTAAACAGCATTCTTGCCTTTCCCTGAGGAGGTGACTTCAGTGTCTCACATGTTCCTTCATGTACCCCCTTTTC
TCTATGGCATATAAGCCCTGGGTCGGGGGGTAACTGTGGGGATCACTGCCTGAGACATGGCTTCTGTGCATAAGTCCCTATTAAATGTTTCTTTCTGACA
AA
  • refseq_C3orf17.F1 (forward primer)
  • refseq_C3orf17.R1 (reverse primer)
  • Single exon skipping

Frame 0

TSGRWTCSRVAPPFVSPPASHDGCGAAGPGAVE
PCENP*GGEPQRSDSAEPRRGPWLSNV*SV*KT*
IWRAQFKTCLSCFLPMKISP*LPKYVLSPVSQW
WSWC**RFWEPASCCSACWTAAAKLFF*L*NI*
VCKSSLF*TL*WLGW*AGYGFSIKVS*KG*FCYM
SLCLDCFKRSLGFNQCLTSKILPFLLISLNF*D
SHILKPLRKKCL*LLQLKE*INC*INCF**MSS
HQEPVKKPCLEFQKKLNK*RSMYRIMWILDSQ*R
IRESSKKSHQNLM*GLSATS*NTKLLRRPVLIL
NVLNPD*RQPSILLRK**ELLMPKVLCKDSERL
SPSHNFLKKSRWQLYGAGAKNSRLRPFFWVTNFL
KATGLNIWKLKVLVCQRN*SA*KRLFATTFFVA
QVSKLQSII*DREDHRINFYGDKGNHRESCSRL
F*GKFSSSLKGLGRVLQIPVLSGDSHTVLCIELI
STLTVSSS*IVEFQCLSYKLRRK*FMKILEASM
KMKLIRGR*CK*IKTVHQEPLRRQMTLMIFLL*
WEFRCSFICETFK*LTRLVQCSK*NC*DLEVPPG
LTEELL*CSIAQEFISVQSTFKVALCHFINKYF
FFGFNYMSGVNMKNLPQPTLEKQM*FLE*G**V
SEKLNNFMTY*AI**SFAKTIVS*GK*GAGVGDS
KETPERSIWSYWLHYT*VRL*ILKFVAPDIYVF
LFICWN*STRFATACITRDWLQPLFLFICLAYL
ILK*VWSSPKRFNFIYLGTSD*FQINLFTKRQ*G
GLWLHMKYNRITGR*ESKMKK*G*EIQSRPRNE
ARTFIIWC*CCHLIGDVARTVALRGVHLEYFQI
MM*SLR*VSFQCSNFTRIRLLSFSSALNFCYIEI
*RKRK*YLSMYRILLFLSHPIITEVISLPFFIW
LCSFPVCLLHFLSYLYQWFSNFRLFENHLVGC*
KMQISPFPETDKQGPETCIFNMEPK*F*SRWPPN
PVI*TLL*LLS*SLK*TGLILNL*SVFIFLLAF
YQVVVSI*CLKIQTIPRRFHSRQV*KWPLTSKC
PCVQ*ISFTYWEIEGKLVRELVYSFVDHRAYLLR
VVYIHAPSPLSSLF*YFI*LIQKKTPWELGDLR
FSLASPTCGFTELVSFLRLRFLDLKWNFFKNET
FIAEINFGAYLSLEKPGWFSELRTI*HGLGCQSS
ICFQIHSCSFGVSSSPLIFPSSALYYSGKMAKK
LHMCPQNDNLYVYKEFSILYQLLFPNKSKYLTF
KKMQSKVYQTVTYLVNEIRNYCQGHRSELLLLLF
FETESCAVAQAGAQWYDLSSLHPPPPGLKLFSH
LSRLSS*HYRHHHALANFLKTLCRDRVSPCCLG
WSQTPGLKGSTHFGLPKCWDYPHPSTNFITSDGI
RTHRPFIRAVFETSCVSLPILLAFILKSLLWQP
VSSRAEPCLLLWGCLTSCPWASHQLQELPSTWV
CTTWKRCGATPSPPQRNSQAQYEAKVTSILDANL
PC*LLITSSWNAL*DFYFYLLKEHTHTHTL*IL
ALSKL*AKL*TAFLPFPEEVTSVSHMFLHVPPF
LYGI*ALGRGVTVGITA*DMASVHKSLLNVSF*Q

Frame 1

PPVAGRALA*LRLS*VPPPRMMAAVPPGLEPWN
RVRIPKAGNRSAVTVQNPGAALG*AMFKAFEKH
EFGGLNSRPV*VVFFQ*KSALNYQSMCCPQSASG
GVGVDEGFGSLQVVAPLVGLLLQNFSFDCETSR
FARVHYFKPCDGWAGEQVMGSL*RCLKKVDFVI
*AFVWIASRGR*DSTNALLQRFYLSF*YH*IFRT
AIF*SL*EKNAYSFCS*RNK*IAK*TVFNK*AV
TKSQ*RNLAWNFKKS*TNEDQCTE*CGSWTASK
E*ESLQRRVIRI*CEGFLQPAETQSYSGDQF*F*
MFSIQTKDNQVFFSESDRNSSCQKFCAKIPRG*
VLHTTF*RNPDGSCMVQEQKTQGSGHFSG*QTS
*KQPA*TSGSSRY*FAKETRVHKNVYLQPPSSWL
RYQNFKASSETEKITE*IFTETKETTEKVAVDS
FKGNSAVLSRDSEECYRYQC*VETLTLYCA*N*
SLP*Q*AALE*WSFNACHTN*GENDS*KS*RHP*
K*N*FVDGDANK*KQYIRNH*GDR*H**YFCFN
GSLDVRSYVRLLSD*QG*FSVLSRTAKIWKYHL
DSQRSCFSAALHRSSFQFSLHLK*HCVISSTSIS
FLASTTCQVLI*KICHSLPLRSRCNSLSEASKS
LKN*IIL*LTRQFNNRLQKPLCLEVNEERGWEI
ARKHLKGQFGLIGSIIHRSASEY*SL*LLTFMFS
CLYVGIDQQGLPLLVLPGTGYNHYFSSFAWLIS
Y*SEFGVLQNVLILFIWVPVIDSK*TFLQKGNK
VVYGYI*NIIG*QVDEKVK*KNEVRRYKVDPGMR
LEHL*FGVDAVI**GMLLVLLL*GEYILSIFK*
*CEV*GRYLSNVLTLLELGY*ASLVPLTFVT*R
FKEKESSTLVCTASFFSSATLS*QKLFPFHSLFG
FVLSLSAFSIFSVISTSGSQTLDFLRITWWVAK
RCRLALSQRLINRGQKPAFLTWSPSDFDPGGPQ
IQLYKHYFSFSPEALSKLV*FLICSQFSYSFWLS
TKW*YPFSALKYRQYQGDFILDKSRNGH*LLSV
LVFNEYPLPIGK*KVN**ENWCIALWTIGLIC*
ELSIFMHPVP*VASFNTSFS*FRKKLPGN*EI*G
SVLLLLLVASLS*SVSSGLGFWI*SGIFSRMKL
L*LK*TLELIFHWKNQGGSVS*GQYSMDWAASL
AFVSKFIHVLLEYLHLLSFSHLLPFTILGKWQKS
FTCVHKMTICMFIRNSVFCISYFFQINPNI*HL
KKCNLKYTKL*LT**MRLETIVRVIVVNYYYYY
FLRQSLVLLPRLEHSGMISVHCTLHLLGSSYSPT
SAA*VADTIGTTMPWLIF*KLCVEIGFHHVA*A
GLKLLDSRDPPTLVSQSAGITHILVPTLSLLMA
LGHTGHL*EQCLRPPVCHFQSSWPSY*SHCCGNQ
FQAGLNLASCSGDVSLPAPGLLTSYRNCLALGY
VQPGRGVGPPHPLLKEIHRHSMRLK*LPSWMLI
YHVDF**PVPGMPSKISTFIY*KNTHTHTHCKSW
P*ASYRLSCKQHSCLSLRR*LQCLTCSFMYPLF
SMAYKPWVGG*LWGSLPETWLLCISPY*MFLSD
K

Frame 2

LRSLDVLSRSSAFRKSPRLA*WLRCRRAWSRGT
V*ESLRRGTAAQ*QCRTPARPLVEQCLKRLKNM
NLEGSIQDLFELFSSNENQPLTTKVCVVPSQPV
VELVLMKVLGACKLLLRLLDCCCKTFLLTVKHLG
LQEFIILNLVMVGLVSRLWVLYKGVLKRLILLY
EPLFGLLQEVARIQPMPYFKDFTFPSDITEFLG
QPYFEAFKKKMPIAFAAKGINKLLNKLFLINEQS
PRASEETLLGISKKAKQMKINVQNNVDLGQPVK
NKRVFKEESSEFDVRAFCNQLKHKATQETSFDF
KCSQSRLKTTKYSSQKVIGTPHAKSFVQRFREAE
SFTQLSEEIQMAVVWCRSKKLKAQAIFLGNKLL
KSNRLKHLEAQGTSLPKKLECIKTSICNHLLRG
SGIKTSKHHLRQRRSQNKFLRRQRKPQRKLQSTL
LREIQQFSQGTRKSATDTSAKWRLSHCTVHRTD
LYPNSKQLLNSGVSMPVIQTKEKMIHENLRGIH
ENETDSWTVMQINKNSTSGTIKETDDIDDIFALM
GV*MFVHM*DF*VINKASSVF*VELLRSGSTTW
THRGAALVQHCTGVHFSSVYI*SSIVSFHQQVF
LFWLQLHVRC*YEKSATAYP*EADVIP*VRLVSL
*KTE*FYDLLGNLIIVCKNHCVLR*MRSGGGR*
QGNT*KVNLVLLAPLYIGPPLNTKVCSS*HLCF
LVYMLELINKVCHCLYYQGLVTTIISLHLLGLSH
IKVSLEFSKTF*FYLFGYQ*LIPNKPFYKKAIR
WFMVTYEI**DNR*MRK*NEKMRLGDTK*TQE*
G*NIYNLVLMLSSDRGCCSYCCSEGSTS*VFSNN
DVKFEVGIFPMF*LY*N*VIKLL*CP*LLLHRD
LKKKKVVP*YVPHPSFPQPPYHNRSYFPSILYL
ALFFPCLPSPFSQLSLPVVLKL*TF*ESPGGLLK
DAD*PFPRD**TGARNLHF*HGAQVILIQVAPK
SSYINTTLASLLKP*VNWFNS*SVVSFHIPFGF
LPSGSIHLVP*NTDNTKEISF*TSLEMAINF*VS
LCSMNILYLLGNRR*ISKRTGV*LCGP*GLFAE
SCLYSCTQSPK*PLLILHLADSEKNSLGIRRFK
VQSCFSYLWLH*ASQFPQA*VSGFEVEFFQE*NF
YS*NKLWSLSFIGKTRVVQ*VKDNIAWTGLPV*
HLFPNSFMFFWSIFISSHFPIFCPLLFWENGKK
ASHVSTK*QFVCL*GIQYSVSVTFSK*IQIFNI*
KNAI*SIPNCNLPSK*D*KLLSGSS**IIIIII
F*DRVLCCCPGWSTVV*SQFTAPSTSWAQAILP
PQPPE*LTL*APPCPG*FFKNFV*R*GFTMLLRL
VSNSWTQGIHPLWSPKVLGLPTS*YQLYHF*WH
*DTQAIYKSSV*DLLCVTSNPLGLHTKVTAVAT
SFKQG*TLPLALGMSHFLPLGFSPATGTA*HLGM
YNLEEVWGHPIPSSKKFTGTV*G*SNFHLGC*S
TMLTSDNQFLECPLRFLLLSIKRTHTHTHTVNP
GLKQVIG*VVNSILAFP*GGDFSVSHVPSCTPFS
LWHISPGSGGNCGDHCLRHGFCA*VPIKCFFLT

Sequence NM_015412

ACCTCCGGTCGCTGGACGTGCTCTCGCGTAGCTCCGCCTTTCGTAAGTCCCCCCGCCTCGCATGATGGCTGCGGTGCCGCCGGGCCTGGAGCCGTGGAAC
CGTGTGAGAATCCCTAAGGCGGGGAACCGCAGCGCAGTGACAGTGCAGAACCCCGGCGCGGCCCTTGACCTTTGCATTGCAGCTGTAATTAAAGAATGCC
ATCTCGTCATACTGTCGCTGAAGAGCCAAACCTTAGATGCAGAAACAGATGTGTTATGTGCAGTCCTTTACAGCAATCACAACAGAATGGGCCGCCACAA
ACCCCATTTGGCCCTCAAACAGGTTGAGCAATGTTTAAAGCGTTTGAAAAACATGAATTTGGAGGGCTCAATTCAAGACCTGTTTGAGTTGTTTTCTTCC
AATGAAAATCAGCCCTTAACTACCAAAGTATGTGTTGTCCCCAGTCAGCCAGTGGTGGAGTTGGTGTTGATGAAGGTTTTGGGAGCCTGCAAGTTGTTGC
TCCGCTTGTTGGACTGCTGCTGCAAAACTTTTCTTTTGACTGTGAAACATCTAGGTTTGCAAGAGTTCATTATTTTAAACCTTGTGATGGTTGGGCTGGT
GAGCAGGTTATGGGTTCTCTATAAAGGTGTCTTAAAAAGGTTGATTTTGTTATATGAGCCTTTGTTTGGATTGCTTCAAGAGGTCGCTAGGATTCAACCA
ATGCCTTACTTCAAAGATTTTACCTTTCCTTCTGATATCACTGAATTTTTAGGACAGCCATATTTTGAAGCCTTTAAGAAAAAAATGCCTATAGCTTTTG
CAGCTAAAGGAATAAATAAATTGCTAAATAAACTGTTTTTAATAAATGAGCAGTCACCAAGAGCCAGTGAAGAAACCTTGCTTGGAATTTCAAAAAAAGC
TAAACAAATGAAGATCAATGTACAGAATAATGTGGATCTTGGACAGCCAGTAAAGAATAAGAGAGTCTTCAAAGAAGAGTCATCAGAATTTGATGTGAGG
GCTTTCTGCAACCAGCTGAAACACAAAGCTACTCAGGAGACCAGTTTTGATTTTAAATGTTCTCAATCCAGACTAAAGACAACCAAGTATTCTTCTCAGA
AAGTGATAGGAACTCCTCATGCCAAAAGTTTTGTGCAAAGATTCCGAGAGGCTGAGTCCTTCACACAACTTTCTGAAGAAATCCAGATGGCAGTTGTATG
GTGCAGGAGCAAAAAACTCAAGGCTCAGGCCATTTTTCTGGGTAACAAACTTCTTAAAAGCAACCGGCTTAAACATCTGGAAGCTCAAGGTACTAGTTTG
CCAAAGAAACTAGAGTGCATAAAAACGTCTATTTGCAACCACCTTCTTCGTGGCTCAGGTATCAAAACTTCAAAGCATCATCTGAGACAGAGAAGATCAC
AGAATAAATTTTTACGGAGACAAAGGAAACCACAGAGAAAGTTGCAGTCGACTCTTTTAAGGGAAATTCAGCAGTTCTCTCAAGGGACTCGGAAGAGTGC
TACAGATACCAGTGCTAAGTGGAGACTCTCACACTGTACTGTGCATAGAACTGATCTCTACCCTAACAGTAAGCAGCTCTTGAATAGTGGAGTTTCAATG
CCTGTCATACAAACTAAGGAGAAAATGATTCATGAAAATCTTAGAGGCATCCATGAAAATGAAACTGATTCGTGGACGGTGATGCAAATAAATAAAAACA
GTACATCAGGAACCATTAAGGAGACAGATGACATTGATGATATTTTTGCTTTAATGGGAGTTTAGATGTTCGTTCATATGTGAGACTTTTAAGTGATTAA
CAAGGCTAGTTCAGTGTTCTAAGTAGAACTGCTAAGATCTGGAAGTACCACCTGGACTCACAGAGGAGCTGCTTTAGTGCAGCATTGCACAGGAGTTCAT
TTCAGTTCAGTCTACATTTAAAGTAGCATTGTGTCATTTCATCAACAAGTATTTCTTTTTTGGCTTCAACTACATGTCAGGTGTTAATATGAAAAATCTG
CCACAGCCTACCCTTGAGAAGCAGATGTAATTCCTTGAGTGAGGCTAGTAAGTCTCTGAAAAACTGAATAATTTTATGACTTACTAGGCAATTTAATAAT
CGTTTGCAAAAACCATTGTGTCTTGAGGTAAATGAGGAGCGGGGGTGGGAGATAGCAAGGAAACACCTGAAAGGTCAATTTGGTCTTATTGGCTCCATTA
TACATAGGTCCGCCTCTGAATACTAAAGTTTGTAGCTCCTGACATTTATGTTTTCTTGTTTATATGTTGGAATTGATCAACAAGGTTTGCCACTGCTTGT
ATTACCAGGGACTGGTTACAACCATTATTTCTCTTCATTTGCTTGGCTTATCTCATATTAAAGTGAGTTTGGAGTTCTCCAAAACGTTTTAATTTTATTT
ATTTGGGTACCAGTGATTGATTCCAAATAAACCTTTTTACAAAAAGGCAATAAGGTGGTTTATGGTTACATATGAAATATAATAGGATAACAGGTAGATG
AGAAAGTAAAATGAAAAAATGAGGTTAGGAGATACAAAGTAGACCCAGGAATGAGGCTAGAACATTTATAATTTGGTGTTGATGCTGTCATCTGATAGGG
GATGTTGCTCGTACTGTTGCTCTGAGGGGAGTACATCTTGAGTATTTTCAAATAATGATGTGAAGTTTGAGGTAGGTATCTTTCCAATGTTCTAACTTTA
CTAGAATTAGGTTATTAAGCTTCTCTAGTGCCCTTAACTTTTGTTACATAGAGATTTAAAGAAAAAGAAAGTAGTACCTTAGTATGTACCGCATCCTTCT
TTTCCTCAGCCACCCTATCATAACAGAAGTTATTTCCCTTCCATTCTTTATTTGGCTTTGTTCTTTCCCTGTCTGCCTTCTCCATTTTCTCAGTTATCTC
TACCAGTGGTTCTCAAACTTTAGACTTTTTGAGAATCACCTGGTGGGTTGCTAAAAGATGCAGATTAGCCCTTTCCCAGAGACTGATAAACAGGGGCCAG
AAACCTGCATTTTTAACATGGAGCCCAAGTGATTTTGATCCAGGTGGCCCCCAAATCCAGTTATATAAACACTACTTTAGCTTCTCTCCTGAAGCCTTAA
GTAAACTGGTTTAATTCTTAATCTGTAGTCAGTTTTCATATTCCTTTTGGCTTTCTACCAAGTGGTAGTATCCATTTAGTGCCTTAAAATACAGACAATA
CCAAGGAGATTTCATTCTAGACAAGTCTAGAAATGGCCATTAACTTCTAAGTGTCCTTGTGTTCAATGAATATCCTTTACCTATTGGGAAATAGAAGGTA
AATTAGTAAGAGAACTGGTGTATAGCTTTGTGGACCATAGGGCTTATTTGCTGAGAGTTGTCTATATTCATGCACCCAGTCCCCTAAGTAGCCTCTTTTA
ATACTTCATTTAGCTGATTCAGAAAAAAACTCCCTGGGAATTAGGAGATTTAAGGTTCAGTCTTGCTTCTCCTACTTGTGGCTTCACTGAGCTAGTCAGT
TTCCTCAGGCTTAGGTTTCTGGATTTGAAGTGGAATTTTTTCAAGAATGAAACTTTTATAGCTGAAATAAACTTTGGAGCTTATCTTTCATTGGAAAAAC
CAGGGTGGTTCAGTGAGTTAAGGACAATATAGCATGGACTGGGCTGCCAGTCTAGCATTTGTTTCCAAATTCATTCATGTTCTTTTGGAGTATCTTCATC
TCCTCTCATTTTCCCATCTTCTGCCCTTTACTATTCTGGGAAAATGGCAAAAAAGCTTCACATGTGTCCACAAAATGACAATTTGTATGTTTATAAGGAA
TTCAGTATTCTGTATCAGTTACTTTTTCCAAATAAATCCAAATATTTAACATTTAAAAAAATGCAATCTAAAGTATACCAAACTGTAACTTACCTAGTAA
ATGAGATTAGAAACTATTGTCAGGGTCATCGTAGTGAATTATTATTATTATTATTTTTTGAGACAGAGTCTTGTGCTGTTGCCCAGGCTGGAGCACAGTG
GTATGATCTCAGTTCACTGCACCCTCCACCTCCTGGGCTCAAGCTATTCTCCCACCTCAGCCGCCTGAGTAGCTGACACTATAGGCACCACCATGCCCTG
GCTAATTTTTTAAAAACTTTGTGTAGAGATAGGGTTTCACCATGTTGCTTAGGCTGGTCTCAAACTCCTGGACTCAAGGGATCCACCCACTTTGGTCTCC
CAAAGTGCTGGGATTACCCACATCCTAGTACCAACTTTATCACTTCTGATGGCATTAGGACACACAGGCCATTTATAAGAGCAGTGTTTGAGACCTCCTG
TGTGTCACTTCCAATCCTCTTGGCCTTCATACTAAAGTCACTGCTGTGGCAACCAGTTTCAAGCAGGGCTGAACCTTGCCTCTTGCTCTGGGGATGTCTC
ACTTCCTGCCCCTGGGCTTCTCACCAGCTACAGGAACTGCCTAGCACTTGGGTATGTACAACCTGGAAGAGGTGTGGGGCCACCCCATCCCCTCCTCAAA
GAAATTCACAGGCACAGTATGAGGCTAAAGTAACTTCCATCTTGGATGCTAATCTACCATGTTGACTTCTGATAACCAGTTCCTGGAATGCCCTCTAAGA
TTTCTACTTTTATCTATTAAAAGAACACACACACACACACACACTGTAAATCCTGGCCTTAAGCAAGTTATAGGCTAAGTTGTAAACAGCATTCTTGCCT
TTCCCTGAGGAGGTGACTTCAGTGTCTCACATGTTCCTTCATGTACCCCCTTTTCTCTATGGCATATAAGCCCTGGGTCGGGGGGTAACTGTGGGGATCA
CTGCCTGAGACATGGCTTCTGTGCATAAGTCCCTATTAAATGTTTCTTTCTGACAAA
  • refseq_C3orf17.F1 (forward primer)
  • refseq_C3orf17.R1 (reverse primer)
  • Single exon skipping

Frame 0

TSGRWTCSRVAPPFVSPPASHDGCGAAGPGAVE
PCENP*GGEPQRSDSAEPRRGP*PLHCSCN*RMP
SRHTVAEEPNLRCRNRCVMCSPLQQSQQNGPPQ
TPFGPQTG*AMFKAFEKHEFGGLNSRPV*VVFF
Q*KSALNYQSMCCPQSASGGVGVDEGFGSLQVVA
PLVGLLLQNFSFDCETSRFARVHYFKPCDGWAG
EQVMGSL*RCLKKVDFVI*AFVWIASRGR*DST
NALLQRFYLSF*YH*IFRTAIF*SL*EKNAYSFC
S*RNK*IAK*TVFNK*AVTKSQ*RNLAWNFKKS
*TNEDQCTE*CGSWTASKE*ESLQRRVIRI*CE
GFLQPAETQSYSGDQF*F*MFSIQTKDNQVFFSE
SDRNSSCQKFCAKIPRG*VLHTTF*RNPDGSCM
VQEQKTQGSGHFSG*QTS*KQPA*TSGSSRY*F
AKETRVHKNVYLQPPSSWLRYQNFKASSETEKIT
E*IFTETKETTEKVAVDSFKGNSAVLSRDSEEC
YRYQC*VETLTLYCA*N*SLP*Q*AALE*WSFN
ACHTN*GENDS*KS*RHP*K*N*FVDGDANK*KQ
YIRNH*GDR*H**YFCFNGSLDVRSYVRLLSD*
QG*FSVLSRTAKIWKYHLDSQRSCFSAALHRSS
FQFSLHLK*HCVISSTSISFLASTTCQVLI*KIC
HSLPLRSRCNSLSEASKSLKN*IIL*LTRQFNN
RLQKPLCLEVNEERGWEIARKHLKGQFGLIGSI
IHRSASEY*SL*LLTFMFSCLYVGIDQQGLPLLV
LPGTGYNHYFSSFAWLISY*SEFGVLQNVLILF
IWVPVIDSK*TFLQKGNKVVYGYI*NIIG*QVD
EKVK*KNEVRRYKVDPGMRLEHL*FGVDAVI**G
MLLVLLL*GEYILSIFK**CEV*GRYLSNVLTL
LELGY*ASLVPLTFVT*RFKEKESSTLVCTASF
FSSATLS*QKLFPFHSLFGFVLSLSAFSIFSVIS
TSGSQTLDFLRITWWVAKRCRLALSQRLINRGQ
KPAFLTWSPSDFDPGGPQIQLYKHYFSFSPEAL
SKLV*FLICSQFSYSFWLSTKW*YPFSALKYRQY
QGDFILDKSRNGH*LLSVLVFNEYPLPIGK*KV
N**ENWCIALWTIGLIC*ELSIFMHPVP*VASF
NTSFS*FRKKLPGN*EI*GSVLLLLLVASLS*SV
SSGLGFWI*SGIFSRMKLL*LK*TLELIFHWKN
QGGSVS*GQYSMDWAASLAFVSKFIHVLLEYLH
LLSFSHLLPFTILGKWQKSFTCVHKMTICMFIRN
SVFCISYFFQINPNI*HLKKCNLKYTKL*LT**
MRLETIVRVIVVNYYYYYFLRQSLVLLPRLEHS
GMISVHCTLHLLGSSYSPTSAA*VADTIGTTMPW
LIF*KLCVEIGFHHVA*AGLKLLDSRDPPTLVS
QSAGITHILVPTLSLLMALGHTGHL*EQCLRPP
VCHFQSSWPSY*SHCCGNQFQAGLNLASCSGDVS
LPAPGLLTSYRNCLALGYVQPGRGVGPPHPLLK
EIHRHSMRLK*LPSWMLIYHVDF**PVPGMPSK
ISTFIY*KNTHTHTHCKSWP*ASYRLSCKQHSCL
SLRR*LQCLTCSFMYPLFSMAYKPWVGG*LWGS
LPETWLLCISPY*MFLSDK

Frame 1

PPVAGRALA*LRLS*VPPPRMMAAVPPGLEPWN
RVRIPKAGNRSAVTVQNPGAALDLCIAAVIKEC
HLVILSLKSQTLDAETDVLCAVLYSNHNRMGRHK
PHLALKQVEQCLKRLKNMNLEGSIQDLFELFSS
NENQPLTTKVCVVPSQPVVELVLMKVLGACKLL
LRLLDCCCKTFLLTVKHLGLQEFIILNLVMVGLV
SRLWVLYKGVLKRLILLYEPLFGLLQEVARIQP
MPYFKDFTFPSDITEFLGQPYFEAFKKKMPIAF
AAKGINKLLNKLFLINEQSPRASEETLLGISKKA
KQMKINVQNNVDLGQPVKNKRVFKEESSEFDVR
AFCNQLKHKATQETSFDFKCSQSRLKTTKYSSQ
KVIGTPHAKSFVQRFREAESFTQLSEEIQMAVVW
CRSKKLKAQAIFLGNKLLKSNRLKHLEAQGTSL
PKKLECIKTSICNHLLRGSGIKTSKHHLRQRRS
QNKFLRRQRKPQRKLQSTLLREIQQFSQGTRKSA
TDTSAKWRLSHCTVHRTDLYPNSKQLLNSGVSM
PVIQTKEKMIHENLRGIHENETDSWTVMQINKN
STSGTIKETDDIDDIFALMGV*MFVHM*DF*VIN
KASSVF*VELLRSGSTTWTHRGAALVQHCTGVH
FSSVYI*SSIVSFHQQVFLFWLQLHVRC*YEKS
ATAYP*EADVIP*VRLVSL*KTE*FYDLLGNLII
VCKNHCVLR*MRSGGGR*QGNT*KVNLVLLAPL
YIGPPLNTKVCSS*HLCFLVYMLELINKVCHCL
YYQGLVTTIISLHLLGLSHIKVSLEFSKTF*FYL
FGYQ*LIPNKPFYKKAIRWFMVTYEI**DNR*M
RK*NEKMRLGDTK*TQE*G*NIYNLVLMLSSDR
GCCSYCCSEGSTS*VFSNNDVKFEVGIFPMF*LY
*N*VIKLL*CP*LLLHRDLKKKKVVP*YVPHPS
FPQPPYHNRSYFPSILYLALFFPCLPSPFSQLS
LPVVLKL*TF*ESPGGLLKDAD*PFPRD**TGAR
NLHF*HGAQVILIQVAPKSSYINTTLASLLKP*
VNWFNS*SVVSFHIPFGFLPSGSIHLVP*NTDN
TKEISF*TSLEMAINF*VSLCSMNILYLLGNRR*
ISKRTGV*LCGP*GLFAESCLYSCTQSPK*PLL
ILHLADSEKNSLGIRRFKVQSCFSYLWLH*ASQ
FPQA*VSGFEVEFFQE*NFYS*NKLWSLSFIGKT
RVVQ*VKDNIAWTGLPV*HLFPNSFMFFWSIFI
SSHFPIFCPLLFWENGKKASHVSTK*QFVCL*G
IQYSVSVTFSK*IQIFNI*KNAI*SIPNCNLPSK
*D*KLLSGSS**IIIIIIF*DRVLCCCPGWSTV
V*SQFTAPSTSWAQAILPPQPPE*LTL*APPCP
G*FFKNFV*R*GFTMLLRLVSNSWTQGIHPLWSP
KVLGLPTS*YQLYHF*WH*DTQAIYKSSV*DLL
CVTSNPLGLHTKVTAVATSFKQG*TLPLALGMS
HFLPLGFSPATGTA*HLGMYNLEEVWGHPIPSSK
KFTGTV*G*SNFHLGC*STMLTSDNQFLECPLR
FLLLSIKRTHTHTHTVNPGLKQVIG*VVNSILA
FP*GGDFSVSHVPSCTPFSLWHISPGSGGNCGDH
CLRHGFCA*VPIKCFFLT

Frame 2

LRSLDVLSRSSAFRKSPRLA*WLRCRRAWSRGT
V*ESLRRGTAAQ*QCRTPARPLTFALQL*LKNA
ISSYCR*RAKP*MQKQMCYVQSFTAITTEWAAT
NPIWPSNRLSNV*SV*KT*IWRAQFKTCLSCFLP
MKISP*LPKYVLSPVSQWWSWC**RFWEPASCC
SACWTAAAKLFF*L*NI*VCKSSLF*TL*WLGW
*AGYGFSIKVS*KG*FCYMSLCLDCFKRSLGFNQ
CLTSKILPFLLISLNF*DSHILKPLRKKCL*LL
QLKE*INC*INCF**MSSHQEPVKKPCLEFQKK
LNK*RSMYRIMWILDSQ*RIRESSKKSHQNLM*G
LSATS*NTKLLRRPVLILNVLNPD*RQPSILLR
K**ELLMPKVLCKDSERLSPSHNFLKKSRWQLY
GAGAKNSRLRPFFWVTNFLKATGLNIWKLKVLVC
QRN*SA*KRLFATTFFVAQVSKLQSII*DREDH
RINFYGDKGNHRESCSRLF*GKFSSSLKGLGRV
LQIPVLSGDSHTVLCIELISTLTVSSS*IVEFQC
LSYKLRRK*FMKILEASMKMKLIRGR*CK*IKT
VHQEPLRRQMTLMIFLL*WEFRCSFICETFK*L
TRLVQCSK*NC*DLEVPPGLTEELL*CSIAQEFI
SVQSTFKVALCHFINKYFFFGFNYMSGVNMKNL
PQPTLEKQM*FLE*G**VSEKLNNFMTY*AI**
SFAKTIVS*GK*GAGVGDSKETPERSIWSYWLHY
T*VRL*ILKFVAPDIYVFLFICWN*STRFATAC
ITRDWLQPLFLFICLAYLILK*VWSSPKRFNFI
YLGTSD*FQINLFTKRQ*GGLWLHMKYNRITGR*
ESKMKK*G*EIQSRPRNEARTFIIWC*CCHLIG
DVARTVALRGVHLEYFQIMM*SLR*VSFQCSNF
TRIRLLSFSSALNFCYIEI*RKRK*YLSMYRILL
FLSHPIITEVISLPFFIWLCSFPVCLLHFLSYL
YQWFSNFRLFENHLVGC*KMQISPFPETDKQGP
ETCIFNMEPK*F*SRWPPNPVI*TLL*LLS*SLK
*TGLILNL*SVFIFLLAFYQVVVSI*CLKIQTI
PRRFHSRQV*KWPLTSKCPCVQ*ISFTYWEIEG
KLVRELVYSFVDHRAYLLRVVYIHAPSPLSSLF*
YFI*LIQKKTPWELGDLRFSLASPTCGFTELVS
FLRLRFLDLKWNFFKNETFIAEINFGAYLSLEK
PGWFSELRTI*HGLGCQSSICFQIHSCSFGVSSS
PLIFPSSALYYSGKMAKKLHMCPQNDNLYVYKE
FSILYQLLFPNKSKYLTFKKMQSKVYQTVTYLV
NEIRNYCQGHRSELLLLLFFETESCAVAQAGAQW
YDLSSLHPPPPGLKLFSHLSRLSS*HYRHHHAL
ANFLKTLCRDRVSPCCLGWSQTPGLKGSTHFGL
PKCWDYPHPSTNFITSDGIRTHRPFIRAVFETSC
VSLPILLAFILKSLLWQPVSSRAEPCLLLWGCL
TSCPWASHQLQELPSTWVCTTWKRCGATPSPPQ
RNSQAQYEAKVTSILDANLPC*LLITSSWNAL*D
FYFYLLKEHTHTHTL*ILALSKL*AKL*TAFLP
FPEEVTSVSHMFLHVPPFLYGI*ALGRGVTVGI
TA*DMASVHKSLLNVSF*Q

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