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Sequence NM_001039375
AAAAAGCGCCGGCAATTGGGGTCGCAGCTGGAGATGCTGCGGCCGGCAGGGCTCTGGCGCTTATGTCGGCGACCTTGGGCGGCGAGGGTCCCAGCGGAGAATCTTGGCCGTAGGGAAGTCACCTCTGGTGTCTCTCCCCGCGGTAGCACCTCGCCCAGAACCCTGAATATTTTCGACCGGGATTTGAAAAGGAAACAGAA
GAACTGGGCAGCCCGGCAGCCCGAGCCGACCAAATTTGACTACCTGAAGGAGGAGGTTGGAAGTCGGATCGCAGACCGTGTATATGACATACCCAGAAAT
TTCCCCCTTGCTTTGGATCTTGGTTGTGGAAGAGGTTACATTGCACAATATTTGAATAAGCTTCAGTTATTCCATTGCAGGAAACTATTGGAAAGTTTTT
CCAAGCTGACATTGCAGAAAATGCTTTGTTTGCATTGGGTGAATGACCTTCCTAGAGCACTTGAGCAGATTCATTATATTTTAAAACCAGATGGAGTGTT
TATCGGTGCAATGTTTGGAGGCGACACACTCTATGAACTTCGGTGTTCCTTACAGTTAGCGGAAACGGAAAGGGAAGGAGGATTTTCTCCACACATTTCT
CCTTTCACTGCTGTCAATGACCTGGGACATCTGCTTGGGAGAGCTGGCTTTAATACTCTGACTGTGGACACTGATGAAATTCAAGTTAACTATCCTGGAA
TGTTTGAATTGATGGAAGATTTACAAGGTATGGGTGAGAGTAACTGTGCTTGGAATAGAAAAGCCCTGCTGCATCGAGACACAATGCTGGCAGCTGCGGC
AGTGTACAGAGAAATGTACAGAAATGAAGATGGTTCAGTACCTGCTACATACCAGATCTATTACATGATAGGATGGAAATATCATGAGTCACAGGCAAGA
CCAGCTGAAAGAGGTTCCGCAACTGTGTCATTTGGAGAGCTAGGAAAAATAAACAACCTTATGCCACCGGGGAAAAAATCACAATAAATATTTATTCAGT
GTTAA
- refseq_C20orf7.F3 (forward primer)
- refseq_C20orf7.R3 (reverse primer)
- Single exon skipping
Frame 0
KKRRQLGSQLEMLRPAGLWRLCRRPWAARVPAENLGRREVTSGVSPRGSTSPRTLNIFDRDLKRKQK
NWAARQPEPTKFDYLKEEVGSRIADRVYDIPRN
FPLALDLGCGRGYIAQYLNKLQLFHCRKLLESF
SKLTLQKMLCLHWVNDLPRALEQIHYILKPDGVF
IGAMFGGDTLYELRCSLQLAETEREGGFSPHIS
PFTAVNDLGHLLGRAGFNTLTVDTDEIQVNYPG
MFELMEDLQGMGESNCAWNRKALLHRDTMLAAAA
VYREMYRNEDGSVPATYQIYYMIGWKYHESQAR
PAERGSATVSFGELGKINNLMPPGKKSQ*IFIQ
C*
Frame 1
KSAGNWGRSWRCCGRQGSGAYVGDLGRRGSQRRILAVGKSPLVSLPAVAPRPEP*IFSTGI*KGNR
RTGQPGSPSRPNLTT*RRRLEVGSQTVYMTYPEI
SPLLWILVVEEVTLHNI*ISFSYSIAGNYWKVF
PS*HCRKCFVCIG*MTFLEHLSRFIIF*NQMEC
LSVQCLEATHSMNFGVPYS*RKRKGKEDFLHTFL
LSLLSMTWDICLGELALIL*LWTLMKFKLTILE
CLN*WKIYKVWVRVTVLGIEKPCCIETQCWQLR
QCTEKCTEMKMVQYLLHTRSIT**DGNIMSHRQD
QLKEVPQLCHLES*EK*TTLCHRGKNHNKYLFS
V
Frame 2
KAPAIGVAAGDAAAGRALALMSATLGGEGPSGESWP*GSHLWCLSPR*HLAQNPEYFRPGFEKETE
ELGSPAARADQI*LPEGGGWKSDRRPCI*HTQK
FPPCFGSWLWKRLHCTIFE*ASVIPLQETIGKFF
QADIAENALFALGE*PS*ST*ADSLYFKTRWSV
YRCNVWRRHTL*TSVFLTVSGNGKGRRIFSTHF
SFHCCQ*PGTSAWESWL*YSDCGH**NSS*LSWN
V*IDGRFTRYG*E*LCLE*KSPAASRHNAGSCG
SVQRNVQK*RWFSTCYIPDLLHDRMEIS*VTGK
TS*KRFRNCVIWRARKNKQPYATGEKITINIYSV
L
Sequence NM_024120
AAAAAGCGCCGGCAATTGGGGTCGCAGCTGGAGATGCTGCGGCCGGCAGGGCTCTGGCGCTTATGTCGGCGACCTTGGGCGGCGAGGGTCCCAGCGGAGAATCTTGGCCGTAGGGAAGTCACCTCTGGTGTCTCTCCCCGCGGTAGCACCTCGCCCAGAACCCTGAATATTTTCGACCGGGATTTGAAAAGGAAACAGAA
GAACTGGGCAGCCCGGCAGCCCGAGCCGACCAAATTTGACTACCTGAAGGAGGAGGTTGGAAGTCGGATCGCAGACCGTGTATATGACATACCCAGAAAT
TTCCCCCTTGCTTTGGATCTTGGTTGTGGAAGAGGTTACATTGCACAATATTTGAATAAGGAAACTATTGGAAAGTTTTTCCAAGCTGACATTGCAGAAA
ATGCTTTGAAAAATTCCTCAGAAACAGAAATACCTACTGTCAGCGTTTTAGCTGATGAAGAATTCCTTCCCTTCAAAGAAAATACATTTGACCTGGTGGT
TAGCAGTTTAAGTTTGCATTGGGTGAATGACCTTCCTAGAGCACTTGAGCAGATTCATTATATTTTAAAACCAGATGGAGTGTTTATCGGTGCAATGTTT
GGAGGCGACACACTCTATGAACTTCGGTGTTCCTTACAGTTAGCGGAAACGGAAAGGGAAGGAGGATTTTCTCCACACATTTCTCCTTTCACTGCTGTCA
ATGACCTGGGACATCTGCTTGGGAGAGCTGGCTTTAATACTCTGACTGTGGACACTGATGAAATTCAAGTTAACTATCCTGGAATGTTTGAATTGATGGA
AGATTTACAAGGTATGGGTGAGAGTAACTGTGCTTGGAATAGAAAAGCCCTGCTGCATCGAGACACAATGCTGGCAGCTGCGGCAGTGTACAGAGAAATG
TACAGAAATGAAGATGGTTCAGTACCTGCTACATACCAGATCTATTACATGATAGGATGGAAATATCATGAGTCACAGGCAAGACCAGCTGAAAGAGGTT
CCGCAACTGTGTCATTTGGAGAGCTAGGAAAAATAAACAACCTTATGCCACCGGGGAAAAAATCACAATAAATATTTATTCAGTGTTAA
- refseq_C20orf7.F3 (forward primer)
- refseq_C20orf7.R3 (reverse primer)
- Single exon skipping
Frame 0
KKRRQLGSQLEMLRPAGLWRLCRRPWAARVPAENLGRREVTSGVSPRGSTSPRTLNIFDRDLKRKQK
NWAARQPEPTKFDYLKEEVGSRIADRVYDIPRN
FPLALDLGCGRGYIAQYLNKETIGKFFQADIAE
NALKNSSETEIPTVSVLADEEFLPFKENTFDLVV
SSLSLHWVNDLPRALEQIHYILKPDGVFIGAMF
GGDTLYELRCSLQLAETEREGGFSPHISPFTAV
NDLGHLLGRAGFNTLTVDTDEIQVNYPGMFELME
DLQGMGESNCAWNRKALLHRDTMLAAAAVYREM
YRNEDGSVPATYQIYYMIGWKYHESQARPAERG
SATVSFGELGKINNLMPPGKKSQ*IFIQC*
Frame 1
KSAGNWGRSWRCCGRQGSGAYVGDLGRRGSQRRILAVGKSPLVSLPAVAPRPEP*IFSTGI*KGNR
RTGQPGSPSRPNLTT*RRRLEVGSQTVYMTYPEI
SPLLWILVVEEVTLHNI*IRKLLESFSKLTLQK
ML*KIPQKQKYLLSAF*LMKNSFPSKKIHLTWW
LAV*VCIG*MTFLEHLSRFIIF*NQMECLSVQCL
EATHSMNFGVPYS*RKRKGKEDFLHTFLLSLLS
MTWDICLGELALIL*LWTLMKFKLTILECLN*W
KIYKVWVRVTVLGIEKPCCIETQCWQLRQCTEKC
TEMKMVQYLLHTRSIT**DGNIMSHRQDQLKEV
PQLCHLES*EK*TTLCHRGKNHNKYLFSV
Frame 2
KAPAIGVAAGDAAAGRALALMSATLGGEGPSGESWP*GSHLWCLSPR*HLAQNPEYFRPGFEKETE
ELGSPAARADQI*LPEGGGWKSDRRPCI*HTQK
FPPCFGSWLWKRLHCTIFE*GNYWKVFPS*HCRK
CFEKFLRNRNTYCQRFS**RIPSLQRKYI*PGG
*QFKFALGE*PS*ST*ADSLYFKTRWSVYRCNV
WRRHTL*TSVFLTVSGNGKGRRIFSTHFSFHCCQ
*PGTSAWESWL*YSDCGH**NSS*LSWNV*IDG
RFTRYG*E*LCLE*KSPAASRHNAGSCGSVQRN
VQK*RWFSTCYIPDLLHDRMEIS*VTGKTS*KRF
RNCVIWRARKNKQPYATGEKITINIYSVL
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