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Sequences

Sequence NM_033169

GGTGCAATACCTGCCTTGCGTCGCCCGTGCCGCTCCAGCCACCGTCAAATCCGGGTAGCGACCCGGGGCGTTTGCAGCGGTGCCGAGGAAGAGGACGGGA ACGGTGTTACGATTGCCTGCGTTTAGGAGGTGGCTGCGTTGTGGGAAAAGCTATCAAGGAAGAAATTGCCAAACCATGTCTTTTTTTCTGTTTTCAGAGT AGTTCACAACAGATCTGAGTGTTTTAATTAAGCATGGAATACAGAAAACAACAAAAAACTTAAGCTTTAATTTCATCTGGAATTCCACAGTTTTCTTAGC TCCCTGGACCCGGTTGACCTGTTGGCTCTTCCCGCTGGCTGCTCTATCACGTGGTGCTCTCCGACTACTCACCCCGAGTGTAAAGAACCTTCGGCTCGCG TGCTTCTGAGCTGCTGTGGATGGCCTCGGCTCTCTGGACTGTCCTTCCGAGTAGGATGTCACTGAGATCCCTCAAATGGAGCCTCCTGCTGCTGTCACTC CTGAGTTTCTTTGTGATGTGGTACCTCAGCCTTCCCCACTACAATGTGATAGAACGCGTGAACTGGATGTACTTCTATGAGTATGAGCCGATTTACAGAC AAGACTTTCACTTCACACTTCGAGAGCATTCAAACTGCTCTCATCAAAATCCATTTCTGGTCATTCTGGTGACCTCCCACCCTTCAGATGTGAAAGCCAG GCAGGCCATTAGAGTTACTTGGGGTGAAAAAAAGTCTTGGTGGGGATATGAGGTTCTTACATTTTTCTTATTAGGCCAAGAGGCTGAAAAGGAAGACAAA ATGTTGGCATTGTCCTTAGAGGATGAACACCTTCTTTATGGTGACATAATCCGACAAGATTTTTTAGACACATATAATAACCTGACCTTGAAAACCATTA TGGCATTCAGGTGGGTAACTGAGTTTTGCCCCAATGCCAAGTACGTAATGAAGACAGACACTGATGTTTTCATCAATACTGGCAATTTAGTGAAGTATCT TTTAAACCTAAACCACTCAGAGAAGTTTTTCACAGGTTATCCTCTAATTGATAATTATTCCTATAGAGGATTTTACCAAAAAACCCATATTTCTTACCAG GAGTATCCTTTCAAGGTGTTCCCTCCATACTGCAGTGGGTTGGGTTATATAATGTCCAGAGATTTGGTGCCAAGGATCTATGAAATGATGGGTCACGTAA AACCCATCAAGTTTGAAGATGTTTATGTCGGGATCTGTTTGAATTTATTAAAAGTGAACATTCATATTCCAGAAGACACAAATCTTTTCTTTCTATATAG AATCCATTTGGATGTCTGTCAACTGAGACGTGTGATTGCAGCCCATGGCTTTTCTTCCAAGGAGATCATCACTTTTTGGCAGGTCATGCTAAGGAACACC ACATGCCATTATTAACTTCACATTCTACAAAAAGCCTAGAAGGACAGGATACTTTGTGGAAAGTGTTAAATAAAGTAGGTACTGTGGAAAATTCATGGGG AGGTCAGTGTGCTGGCTTACACTGAACTGAAACTCATGAAAAACCCAGACTGGAGACTGGAGGGTTACACTTGTGATTTATTAGTCAGGCCCTTCAAAGA TGATATGTGGAGGAATTAAATATAAAGGAATTGGAGGTTTTTGCTAAAGAAATTAATAGGACCAAACAATTTGGACATGTCATTCTGTAGACTAGAATTT CTTAAAAGGGTGTTACTGAGTTATAAGCTCACTAGGCTGTAAAAACAAAACAATGTAGAGTTTTATTTATTGAACAATGTAGTCACTTGAAGGTTTTGTG TATATCTTATGTGGATTACCAATTTAAAAATATATGTAGTTCTGTGTCAAAAAACTTCTTCACTGAAGTTATACTGAACAAAATTTTACCTGTTTTTGGT CATTTATAAAGTACTTCAAGATGTTGCAGTATTTCACAGTTATTATTATTTAAAATTACTTCAACTTTGTGTTTTTAAATGTTTTGACGATTTCAATACA AGATAAAAAGGATAGTGAATCATTCTTTACATGCAAACATTTTCCAGTTACTTAACTGATCAGTTTATTATTGATACATCACTCCATTAATGTAAAGTCA TAGGTCATTATTGCATATCAGTAATCTCTTGGACTTTGTTAAATATTTTACTGTGGTAATATAGAGAAGAATTAAAGCAAGAAAATCTGAAGTATTGTCT TGTTTTTAAAAAATACAGTTCCTAGTGTTTTTAGAAGTCACTTAATTTGTCTCATTTTTCCACCTGGAAATTAGGAATAATGTAGAATGCAAGGCAGTAA TTTCCTTTTGGAAAGGACTCTGAAGGCAGAAAAGAAGGGAGAGAACCTCATGGGCAGAATATTATAAAAAGAGTGTCATATTCCAGCATTTGAATTGGAA AGAGAAGAGTGAAGATCCAAGTTGCATTATTAATCTGCCCTGTGTTTTTTCCTTTTAACAATCAGTTTGAGCTGCTGCTGTTATGAGTTTCTCATCAAGA TGAAAGCCCTAATATGTAAAGTCAAATCCGATTTAAATTTTGTGCTTTTATAGAAAGAAATTTCTTCATAGACGTGGTGATATATCATTTGTTGGACCTG CTAATAGTAGGTCAAAGGGGAGCACTCCTTGCCCCCTGTTCCTGGGTTTATGCAGTTTTCTTTTTAGAGTTTATATAGGGCAAGTGGTTCTTTTTCTCTG AATTACAGGATGGAAAAAGGTCATATCCTTTGTCAGGAAATATAAACTTGAAAGTATGTAGTCAGCTCTTGTAATACTCATATTTATGATTGTCCTATAT GAAAAACAACTTCAGTTAAAACTATAATGTGTGATTCTGTATAACAAGGTGATGTCTGTTTCCCAGGGCTCAGACCTAATCCAGTTATAATAAAATCAAT TAAATGAAATATTCTATAGAATCGATCTATGCCCTTGTTAATCTCCATCCATATAGGAGTCACGTTCTTTAAGACAGATGGTGGTAGTTATTTTTGTGGC ATGGTTAGATTTGACTGGTTTTGCAGAAGATTACAGTTATGTACTGCATAATGACATATACAATAGTGGTCCCATAAAATTATAATGGAGCAGAAAATCT ATTGCCTCATGATGTTTTAGCCGTTTTAATGTCATAGCCTAGTGCATTACTCACGTGTCTGTGGAGATGCTGGTGTAAACAAACCTACTACACTGCCAGT TGTATAAAAGTACAGCACATTCAGTTATGTACAGTATGTAATACTGATAATGACAATAAATGACACCGGTTTGTGTATTTACTTTTTATAA

refseq_B3GALNT1.F3 (forward primer)
refseq_B3GALNT1.R3 (reverse primer)
Single exon skipping

Frame 0

GAIPALRRPCRSSHRQIRVATRGVCSGAEEEDG NGVTIACV*EVAALWEKLSRKKLPNHVFFSVFRV VHNRSECFN*AWNTENNKKLKL*FHLEFHSFLS SLDPVDLLALPAGCSITWCSPTTHPECKEPSAR VLLSCCGWPRLSGLSFRVGCH*DPSNGASCCCHS *VSL*CGTSAFPTTM**NA*TGCTSMSMSRFTD KTFTSHFESIQTALIKIHFWSFW*PPTLQM*KP GRPLELLGVKKSLGGDMRFLHFSY*AKRLKRKTK CWHCP*RMNTFFMVT*SDKIF*THIIT*P*KPL WHSGG*LSFAPMPST**RQTLMFSSILAI**SI F*T*TTQRSFSQVIL*LIIIPIEDFTKKPIFLTR SILSRCSLHTAVGWVI*CPEIWCQGSMK*WVT* NPSSLKMFMSGSV*IY*K*TFIFQKTQIFSFYI ESIWMSVN*DV*LQPMAFLPRRSSLFGRSC*GTP HAIINFTFYKKPRRTGYFVESVK*SRYCGKFMG RSVCWLTLN*NS*KTQTGDWRVTLVIY*SGPSK MICGGIKYKGIGGFC*RN**DQTIWTCHSVD*NF LKGCY*VISSLGCKNKTM*SFIY*TM*SLEGFV YILCGLPI*KYM*FCVKKLLH*SYTEQNFTCFW SFIKYFKMLQYFTVIII*NYFNFVFLNVLTISIQ DKKDSESFFTCKHFPVT*LISLLLIHHSINVKS *VIIAYQ*SLGLC*IFYCGNIEKN*SKKI*SIV LFLKNTVPSVFRSHLICLIFPPGN*E*CRMQGSN FLLERTLKAEKKGENLMGRIL*KECHIPAFELE REE*RSKLHY*SALCFFLLTISLSCCCYEFLIK MKALICKVKSDLNFVLL*KEISS*TW*YIICWTC ***VKGEHSLPPVPGFMQFSF*SLYRASGSFSL NYRMEKGHILCQEI*T*KYVVSSCNTHIYDCPI *KTTSVKTIMCDSV*QGDVCFPGLRPNPVIIKSI K*NIL*NRSMPLLISIHIGVTFFKTDGGSYFCG MVRFDWFCRRLQLCTA**HIQ*WSHKIIMEQKI YCLMMF*PF*CHSLVHYSRVCGDAGVNKPTTLPV V*KYSTFSYVQYVILIMTINDTGLCIYFL*

Frame 1

VQYLPCVARAAPATVKSG*RPGAFAAVPRKRTG TVLRLPAFRRWLRCGKSYQGRNCQTMSFFLFSE *FTTDLSVLIKHGIQKTTKNLSFNFIWNSTVFLA PWTRLTCWLFPLAALSRGALRLLTPSVKNLRLA CF*AAVDGLGSLDCPSE*DVTEIPQMEPPAAVT PEFLCDVVPQPSPLQCDRTRELDVLL*V*ADLQT RLSLHTSRAFKLLSSKSISGHSGDLPPFRCESQ AGH*SYLG*KKVLVGI*GSYIFLIRPRG*KGRQ NVGIVLRG*TPSLW*HNPTRFFRHI**PDLENHY GIQVGN*VLPQCQVRNEDRH*CFHQYWQFSEVS FKPKPLREVFHRLSSN**LFL*RILPKNPYFLP GVSFQGVPSILQWVGLYNVQRFGAKDL*NDGSRK THQV*RCLCRDLFEFIKSEHSYSRRHKSFLSI* NPFGCLSTETCDCSPWLFFQGDHHFLAGHAKEH HMPLLTSHSTKSLEGQDTLWKVLNKVGTVENSWG GQCAGLH*TETHEKPRLETGGLHL*FISQALQR *YVEELNIKELEVFAKEINRTKQFGHVIL*TRI S*KGVTEL*AH*AVKTKQCRVLFIEQCSHLKVLC ISYVDYQFKNICSSVSKNFFTEVILNKILPVFG HL*STSRCCSISQLLLFKITSTLCF*MF*RFQY KIKRIVNHSLHANIFQLLN*SVYY*YITPLM*SH RSLLHISNLLDFVKYFTVVI*RRIKARKSEVLS CF*KIQFLVFLEVT*FVSFFHLEIRNNVECKAV ISFWKGL*RQKRRERTSWAEYYKKSVIFQHLNWK EKSEDPSCIINLPCVFSF*QSV*AAAVMSFSSR *KP*YVKSNPI*ILCFYRKKFLHRRGDISFVGP ANSRSKGSTPCPLFLGLCSFLFRVYIGQVVLFL* ITGWKKVISFVRKYKLESM*SALVILIFMIVLY EKQLQLKL*CVILYNKVMSVSQGSDLIQL**NQ LNEIFYRIDLCPC*SPSI*ESRSLRQMVVVIFVA WLDLTGFAEDYSYVLHNDIYNSGPIKL*WSRKS IAS*CFSRFNVIA*CITHVSVEMLV*TNLLHCQ LYKSTAHSVMYSM*Y***Q*MTPVCVFTFY

Frame 2

CNTCLASPVPLQPPSNPGSDPGRLQRCRGRGRE RCYDCLRLGGGCVVGKAIKEEIAKPCLFFCFQS SSQQI*VF*LSMEYRKQQKT*ALISSGIPQFS* LPGPG*PVGSSRWLLYHVVLSDYSPRV*RTFGSR ASELLWMASALWTVLPSRMSLRSLKWSLLLLSL LSFFVMWYLSLPHYNVIERVNWMYFYEYEPIYR QDFHFTLREHSNCSHQNPFLVILVTSHPSDVKAR QAIRVTWGEKKSWWGYEVLTFFLLGQEAEKEDK MLALSLEDEHLLYGDIIRQDFLDTYNNLTLKTI MAFRWVTEFCPNAKYVMKTDTDVFINTGNLVKYL LNLNHSEKFFTGYPLIDNYSYRGFYQKTHISYQ EYPFKVFPPYCSGLGYIMSRDLVPRIYEMMGHV KPIKFEDVYVGICLNLLKVNIHIPEDTNLFFLYR IHLDVCQLRRVIAAHGFSSKEIITFWQVMLRNT TCHY*LHILQKA*KDRILCGKC*IK*VLWKIHG EVSVLAYTELKLMKNPDWRLEGYTCDLLVRPFKD DMWRN*I*RNWRFLLKKLIGPNNLDMSFCRLEF LKRVLLSYKLTRL*KQNNVEFYLLNNVVT*RFC VYLMWITNLKIYVVLCQKTSSLKLY*TKFYLFLV IYKVLQDVAVFHSYYYLKLLQLCVFKCFDDFNT R*KG**IILYMQTFSSYLTDQFIIDTSLH*CKV IGHYCISVISWTLLNILLW*YREELKQENLKYCL VFKKYSS*CF*KSLNLSHFSTWKLGIM*NARQ* FPFGKDSEGRKEGREPHGQNIIKRVSYSSI*IG KRRVKIQVALLICPVFFPFNNQFELLLL*VSHQD ESPNM*SQIRFKFCAFIERNFFIDVVIYHLLDL LIVGQRGALLAPCSWVYAVFFLEFI*GKWFFFS ELQDGKRSYPLSGNINLKVCSQLL*YSYL*LSYM KNNFS*NYNV*FCITR*CLFPRAQT*SSYNKIN *MKYSIESIYALVNLHPYRSHVL*DRWW*LFLW HG*I*LVLQKITVMYCIMTYTIVVP*NYNGAENL LPHDVLAVLMS*PSALLTCLWRCWCKQTYYTAS CIKVQHIQLCTVCNTDNDNK*HRFVYLLFI

Sequence NM_003781

GCTTCCGGCCGCGGCCCTGGCGCGTGGTCTGCGCGCGCCCGGCCGTGCGTGCGGATGCGGGGAGGCTGCGTGTGTGCGCAGGGAGAGAACGCCGGCCACC TTCCCGCTTCCGAGCTGGGTGCGCGCCGAGCACAGGAGATTGCCTGCGTTTAGGAGGTGGCTGCGTTGTGGGAAAAGCTATCAAGGAAGAAATTGCCAAA CCATGTCTTTTTTTCTGTTTTCAGAGTAGTTCACAACAGATCTGAGTGTTTTAATTAAGCATGGAATACAGAAAACAACAAAAAACTTAAGCTTTAATTT CATCTGGAATTCCACAGTTTTCTTAGCTCCCTGGACCCGGTTGACCTGTTGGCTCTTCCCGCTGGCTGCTCTATCACGTGGTGCTCTCCGACTACTCACC CCGAGTGTAAAGAACCTTCGGCTCGCGTGCTTCTGAGCTGCTGTGGATGGCCTCGGCTCTCTGGACTGTCCTTCCGAGTAGGATGTCACTGAGATCCCTC AAATGGAGCCTCCTGCTGCTGTCACTCCTGAGTTTCTTTGTGATGTGGTACCTCAGCCTTCCCCACTACAATGTGATAGAACGCGTGAACTGGATGTACT TCTATGAGTATGAGCCGATTTACAGACAAGACTTTCACTTCACACTTCGAGAGCATTCAAACTGCTCTCATCAAAATCCATTTCTGGTCATTCTGGTGAC CTCCCACCCTTCAGATGTGAAAGCCAGGCAGGCCATTAGAGTTACTTGGGGTGAAAAAAAGTCTTGGTGGGGATATGAGGTTCTTACATTTTTCTTATTA GGCCAAGAGGCTGAAAAGGAAGACAAAATGTTGGCATTGTCCTTAGAGGATGAACACCTTCTTTATGGTGACATAATCCGACAAGATTTTTTAGACACAT ATAATAACCTGACCTTGAAAACCATTATGGCATTCAGGTGGGTAACTGAGTTTTGCCCCAATGCCAAGTACGTAATGAAGACAGACACTGATGTTTTCAT CAATACTGGCAATTTAGTGAAGTATCTTTTAAACCTAAACCACTCAGAGAAGTTTTTCACAGGTTATCCTCTAATTGATAATTATTCCTATAGAGGATTT TACCAAAAAACCCATATTTCTTACCAGGAGTATCCTTTCAAGGTGTTCCCTCCATACTGCAGTGGGTTGGGTTATATAATGTCCAGAGATTTGGTGCCAA GGATCTATGAAATGATGGGTCACGTAAAACCCATCAAGTTTGAAGATGTTTATGTCGGGATCTGTTTGAATTTATTAAAAGTGAACATTCATATTCCAGA AGACACAAATCTTTTCTTTCTATATAGAATCCATTTGGATGTCTGTCAACTGAGACGTGTGATTGCAGCCCATGGCTTTTCTTCCAAGGAGATCATCACT TTTTGGCAGGTCATGCTAAGGAACACCACATGCCATTATTAACTTCACATTCTACAAAAAGCCTAGAAGGACAGGATACTTTGTGGAAAGTGTTAAATAA AGTAGGTACTGTGGAAAATTCATGGGGAGGTCAGTGTGCTGGCTTACACTGAACTGAAACTCATGAAAAACCCAGACTGGAGACTGGAGGGTTACACTTG TGATTTATTAGTCAGGCCCTTCAAAGATGATATGTGGAGGAATTAAATATAAAGGAATTGGAGGTTTTTGCTAAAGAAATTAATAGGACCAAACAATTTG GACATGTCATTCTGTAGACTAGAATTTCTTAAAAGGGTGTTACTGAGTTATAAGCTCACTAGGCTGTAAAAACAAAACAATGTAGAGTTTTATTTATTGA ACAATGTAGTCACTTGAAGGTTTTGTGTATATCTTATGTGGATTACCAATTTAAAAATATATGTAGTTCTGTGTCAAAAAACTTCTTCACTGAAGTTATA CTGAACAAAATTTTACCTGTTTTTGGTCATTTATAAAGTACTTCAAGATGTTGCAGTATTTCACAGTTATTATTATTTAAAATTACTTCAACTTTGTGTT TTTAAATGTTTTGACGATTTCAATACAAGATAAAAAGGATAGTGAATCATTCTTTACATGCAAACATTTTCCAGTTACTTAACTGATCAGTTTATTATTG ATACATCACTCCATTAATGTAAAGTCATAGGTCATTATTGCATATCAGTAATCTCTTGGACTTTGTTAAATATTTTACTGTGGTAATATAGAGAAGAATT AAAGCAAGAAAATCTGAAGTATTGTCTTGTTTTTAAAAAATACAGTTCCTAGTGTTTTTAGAAGTCACTTAATTTGTCTCATTTTTCCACCTGGAAATTA GGAATAATGTAGAATGCAAGGCAGTAATTTCCTTTTGGAAAGGACTCTGAAGGCAGAAAAGAAGGGAGAGAACCTCATGGGCAGAATATTATAAAAAGAG TGTCATATTCCAGCATTTGAATTGGAAAGAGAAGAGTGAAGATCCAAGTTGCATTATTAATCTGCCCTGTGTTTTTTCCTTTTAACAATCAGTTTGAGCT GCTGCTGTTATGAGTTTCTCATCAAGATGAAAGCCCTAATATGTAAAGTCAAATCCGATTTAAATTTTGTGCTTTTATAGAAAGAAATTTCTTCATAGAC GTGGTGATATATCATTTGTTGGACCTGCTAATAGTAGGTCAAAGGGGAGCACTCCTTGCCCCCTGTTCCTGGGTTTATGCAGTTTTCTTTTTAGAGTTTA TATAGGGCAAGTGGTTCTTTTTCTCTGAATTACAGGATGGAAAAAGGTCATATCCTTTGTCAGGAAATATAAACTTGAAAGTATGTAGTCAGCTCTTGTA ATACTCATATTTATGATTGTCCTATATGAAAAACAACTTCAGTTAAAACTATAATGTGTGATTCTGTATAACAAGGTGATGTCTGTTTCCCAGGGCTCAG ACCTAATCCAGTTATAATAAAATCAATTAAATGAAATATTCTATAGAATCGATCTATGCCCTTGTTAATCTCCATCCATATAGGAGTCACGTTCTTTAAG ACAGATGGTGGTAGTTATTTTTGTGGCATGGTTAGATTTGACTGGTTTTGCAGAAGATTACAGTTATGTACTGCATAATGACATATACAATAGTGGTCCC ATAAAATTATAATGGAGCAGAAAATCTATTGCCTCATGATGTTTTAGCCGTTTTAATGTCATAGCCTAGTGCATTACTCACGTGTCTGTGGAGATGCTGG TGTAAACAAACCTACTACACTGCCAGTTGTATAAAAGTACAGCACATTCAGTTATGTACAGTATGTAATACTGATAATGACAATAAATGACACCGGTTTG TGTATTTACTTTTTATAA

refseq_B3GALNT1.F3 (forward primer)
refseq_B3GALNT1.R3 (reverse primer)
Single exon skipping

Frame 0

ASGRGPGAWSARARPCVRMRGGCVCAQGENAGH LPASELGARRAQEIACV*EVAALWEKLSRKKLPN HVFFSVFRVVHNRSECFN*AWNTENNKKLKL*F HLEFHSFLSSLDPVDLLALPAGCSITWCSPTTH PECKEPSARVLLSCCGWPRLSGLSFRVGCH*DPS NGASCCCHS*VSL*CGTSAFPTTM**NA*TGCT SMSMSRFTDKTFTSHFESIQTALIKIHFWSFW* PPTLQM*KPGRPLELLGVKKSLGGDMRFLHFSY* AKRLKRKTKCWHCP*RMNTFFMVT*SDKIF*TH IIT*P*KPLWHSGG*LSFAPMPST**RQTLMFS SILAI**SIF*T*TTQRSFSQVIL*LIIIPIEDF TKKPIFLTRSILSRCSLHTAVGWVI*CPEIWCQ GSMK*WVT*NPSSLKMFMSGSV*IY*K*TFIFQ KTQIFSFYIESIWMSVN*DV*LQPMAFLPRRSSL FGRSC*GTPHAIINFTFYKKPRRTGYFVESVK* SRYCGKFMGRSVCWLTLN*NS*KTQTGDWRVTL VIY*SGPSKMICGGIKYKGIGGFC*RN**DQTIW TCHSVD*NFLKGCY*VISSLGCKNKTM*SFIY* TM*SLEGFVYILCGLPI*KYM*FCVKKLLH*SY TEQNFTCFWSFIKYFKMLQYFTVIII*NYFNFVF LNVLTISIQDKKDSESFFTCKHFPVT*LISLLL IHHSINVKS*VIIAYQ*SLGLC*IFYCGNIEKN *SKKI*SIVLFLKNTVPSVFRSHLICLIFPPGN* E*CRMQGSNFLLERTLKAEKKGENLMGRIL*KE CHIPAFELEREE*RSKLHY*SALCFFLLTISLS CCCYEFLIKMKALICKVKSDLNFVLL*KEISS*T W*YIICWTC***VKGEHSLPPVPGFMQFSF*SL YRASGSFSLNYRMEKGHILCQEI*T*KYVVSSC NTHIYDCPI*KTTSVKTIMCDSV*QGDVCFPGLR PNPVIIKSIK*NIL*NRSMPLLISIHIGVTFFK TDGGSYFCGMVRFDWFCRRLQLCTA**HIQ*WS HKIIMEQKIYCLMMF*PF*CHSLVHYSRVCGDAG VNKPTTLPVV*KYSTFSYVQYVILIMTINDTGL CIYFL*

Frame 1

LPAAALARGLRAPGRACGCGEAACVRRERTPAT FPLPSWVRAEHRRLPAFRRWLRCGKSYQGRNCQ TMSFFLFSE*FTTDLSVLIKHGIQKTTKNLSFNF IWNSTVFLAPWTRLTCWLFPLAALSRGALRLLT PSVKNLRLACF*AAVDGLGSLDCPSE*DVTEIP QMEPPAAVTPEFLCDVVPQPSPLQCDRTRELDVL L*V*ADLQTRLSLHTSRAFKLLSSKSISGHSGD LPPFRCESQAGH*SYLG*KKVLVGI*GSYIFLI RPRG*KGRQNVGIVLRG*TPSLW*HNPTRFFRHI **PDLENHYGIQVGN*VLPQCQVRNEDRH*CFH QYWQFSEVSFKPKPLREVFHRLSSN**LFL*RI LPKNPYFLPGVSFQGVPSILQWVGLYNVQRFGAK DL*NDGSRKTHQV*RCLCRDLFEFIKSEHSYSR RHKSFLSI*NPFGCLSTETCDCSPWLFFQGDHH FLAGHAKEHHMPLLTSHSTKSLEGQDTLWKVLNK VGTVENSWGGQCAGLH*TETHEKPRLETGGLHL *FISQALQR*YVEELNIKELEVFAKEINRTKQF GHVIL*TRIS*KGVTEL*AH*AVKTKQCRVLFIE QCSHLKVLCISYVDYQFKNICSSVSKNFFTEVI LNKILPVFGHL*STSRCCSISQLLLFKITSTLC F*MF*RFQYKIKRIVNHSLHANIFQLLN*SVYY* YITPLM*SHRSLLHISNLLDFVKYFTVVI*RRI KARKSEVLSCF*KIQFLVFLEVT*FVSFFHLEI RNNVECKAVISFWKGL*RQKRRERTSWAEYYKKS VIFQHLNWKEKSEDPSCIINLPCVFSF*QSV*A AAVMSFSSR*KP*YVKSNPI*ILCFYRKKFLHR RGDISFVGPANSRSKGSTPCPLFLGLCSFLFRVY IGQVVLFL*ITGWKKVISFVRKYKLESM*SALV ILIFMIVLYEKQLQLKL*CVILYNKVMSVSQGS DLIQL**NQLNEIFYRIDLCPC*SPSI*ESRSLR QMVVVIFVAWLDLTGFAEDYSYVLHNDIYNSGP IKL*WSRKSIAS*CFSRFNVIA*CITHVSVEML V*TNLLHCQLYKSTAHSVMYSM*Y***Q*MTPVC VFTFY

Frame 2

FRPRPWRVVCARPAVRADAGRLRVCAGRERRPP SRFRAGCAPSTGDCLRLGGGCVVGKAIKEEIAK PCLFFCFQSSSQQI*VF*LSMEYRKQQKT*ALI SSGIPQFS*LPGPG*PVGSSRWLLYHVVLSDYSP RV*RTFGSRASELLWMASALWTVLPSRMSLRSL KWSLLLLSLLSFFVMWYLSLPHYNVIERVNWMY FYEYEPIYRQDFHFTLREHSNCSHQNPFLVILVT SHPSDVKARQAIRVTWGEKKSWWGYEVLTFFLL GQEAEKEDKMLALSLEDEHLLYGDIIRQDFLDT YNNLTLKTIMAFRWVTEFCPNAKYVMKTDTDVFI NTGNLVKYLLNLNHSEKFFTGYPLIDNYSYRGF YQKTHISYQEYPFKVFPPYCSGLGYIMSRDLVP RIYEMMGHVKPIKFEDVYVGICLNLLKVNIHIPE DTNLFFLYRIHLDVCQLRRVIAAHGFSSKEIIT FWQVMLRNTTCHY*LHILQKA*KDRILCGKC*I K*VLWKIHGEVSVLAYTELKLMKNPDWRLEGYTC DLLVRPFKDDMWRN*I*RNWRFLLKKLIGPNNL DMSFCRLEFLKRVLLSYKLTRL*KQNNVEFYLL NNVVT*RFCVYLMWITNLKIYVVLCQKTSSLKLY *TKFYLFLVIYKVLQDVAVFHSYYYLKLLQLCV FKCFDDFNTR*KG**IILYMQTFSSYLTDQFII DTSLH*CKVIGHYCISVISWTLLNILLW*YREEL KQENLKYCLVFKKYSS*CF*KSLNLSHFSTWKL GIM*NARQ*FPFGKDSEGRKEGREPHGQNIIKR VSYSSI*IGKRRVKIQVALLICPVFFPFNNQFEL LLL*VSHQDESPNM*SQIRFKFCAFIERNFFID VVIYHLLDLLIVGQRGALLAPCSWVYAVFFLEF I*GKWFFFSELQDGKRSYPLSGNINLKVCSQLL* YSYL*LSYMKNNFS*NYNV*FCITR*CLFPRAQ T*SSYNKIN*MKYSIESIYALVNLHPYRSHVL* DRWW*LFLWHG*I*LVLQKITVMYCIMTYTIVVP *NYNGAENLLPHDVLAVLMS*PSALLTCLWRCW CKQTYYTASCIKVQHIQLCTVCNTDNDNK*HRF VYLLFI

Sequence NM_033167

GCTTCCGGCCGCGGCCCTGGCGCGTGGTCTGCGCGCGCCCGGCCGTGCGTGCGGATGCGGGGAGGCTGCGTGTGTGCGCAGGGAGAGAACGCCGGCCACC TTCCCGCTTCCGAGCTGGGTGCGCGCCGAGCACAGGAGATTGCCTGCGTTTAGGAGGTGGCTGCGTTGTGGGAAAAGCTATCAAGGAAGAAATTGCCAAA CCATGTCTTTTTTTCTGTTTTCAGAGTAGTTCACAACAGATCTGAGTGTTTTAATTAAGCATGGAATACAGAAAACAACAAAAAACTTAAGCTTTAATTT CATCTGGAATTCCACAGTTTTCTTAGCTCCCTGGACCCGGTTGACCTGTTGGCTCTTCCCGCTGGCTGCTCTATCACGTGGTGCTCTCCGACTACTCACC CCGAGTGTAAAGTCTTGCTGCTCTGTTGCCCAGGCTGGAGTACAGTGGCAGGATCTCAGCTCACTGCAATCTCCGTCTCTCAGGTTTAAGCAGTTCTCCT GCCTCAGCCTCCCAAGTAGGTGGGACTACAGAACCTTCGGCTCGCGTGCTTCTGAGCTGCTGTGGATGGCCTCGGCTCTCTGGACTGTCCTTCCGAGTAG GATGTCACTGAGATCCCTCAAATGGAGCCTCCTGCTGCTGTCACTCCTGAGTTTCTTTGTGATGTGGTACCTCAGCCTTCCCCACTACAATGTGATAGAA CGCGTGAACTGGATGTACTTCTATGAGTATGAGCCGATTTACAGACAAGACTTTCACTTCACACTTCGAGAGCATTCAAACTGCTCTCATCAAAATCCAT TTCTGGTCATTCTGGTGACCTCCCACCCTTCAGATGTGAAAGCCAGGCAGGCCATTAGAGTTACTTGGGGTGAAAAAAAGTCTTGGTGGGGATATGAGGT TCTTACATTTTTCTTATTAGGCCAAGAGGCTGAAAAGGAAGACAAAATGTTGGCATTGTCCTTAGAGGATGAACACCTTCTTTATGGTGACATAATCCGA CAAGATTTTTTAGACACATATAATAACCTGACCTTGAAAACCATTATGGCATTCAGGTGGGTAACTGAGTTTTGCCCCAATGCCAAGTACGTAATGAAGA CAGACACTGATGTTTTCATCAATACTGGCAATTTAGTGAAGTATCTTTTAAACCTAAACCACTCAGAGAAGTTTTTCACAGGTTATCCTCTAATTGATAA TTATTCCTATAGAGGATTTTACCAAAAAACCCATATTTCTTACCAGGAGTATCCTTTCAAGGTGTTCCCTCCATACTGCAGTGGGTTGGGTTATATAATG TCCAGAGATTTGGTGCCAAGGATCTATGAAATGATGGGTCACGTAAAACCCATCAAGTTTGAAGATGTTTATGTCGGGATCTGTTTGAATTTATTAAAAG TGAACATTCATATTCCAGAAGACACAAATCTTTTCTTTCTATATAGAATCCATTTGGATGTCTGTCAACTGAGACGTGTGATTGCAGCCCATGGCTTTTC TTCCAAGGAGATCATCACTTTTTGGCAGGTCATGCTAAGGAACACCACATGCCATTATTAACTTCACATTCTACAAAAAGCCTAGAAGGACAGGATACTT TGTGGAAAGTGTTAAATAAAGTAGGTACTGTGGAAAATTCATGGGGAGGTCAGTGTGCTGGCTTACACTGAACTGAAACTCATGAAAAACCCAGACTGGA GACTGGAGGGTTACACTTGTGATTTATTAGTCAGGCCCTTCAAAGATGATATGTGGAGGAATTAAATATAAAGGAATTGGAGGTTTTTGCTAAAGAAATT AATAGGACCAAACAATTTGGACATGTCATTCTGTAGACTAGAATTTCTTAAAAGGGTGTTACTGAGTTATAAGCTCACTAGGCTGTAAAAACAAAACAAT GTAGAGTTTTATTTATTGAACAATGTAGTCACTTGAAGGTTTTGTGTATATCTTATGTGGATTACCAATTTAAAAATATATGTAGTTCTGTGTCAAAAAA CTTCTTCACTGAAGTTATACTGAACAAAATTTTACCTGTTTTTGGTCATTTATAAAGTACTTCAAGATGTTGCAGTATTTCACAGTTATTATTATTTAAA ATTACTTCAACTTTGTGTTTTTAAATGTTTTGACGATTTCAATACAAGATAAAAAGGATAGTGAATCATTCTTTACATGCAAACATTTTCCAGTTACTTA ACTGATCAGTTTATTATTGATACATCACTCCATTAATGTAAAGTCATAGGTCATTATTGCATATCAGTAATCTCTTGGACTTTGTTAAATATTTTACTGT GGTAATATAGAGAAGAATTAAAGCAAGAAAATCTGAAGTATTGTCTTGTTTTTAAAAAATACAGTTCCTAGTGTTTTTAGAAGTCACTTAATTTGTCTCA TTTTTCCACCTGGAAATTAGGAATAATGTAGAATGCAAGGCAGTAATTTCCTTTTGGAAAGGACTCTGAAGGCAGAAAAGAAGGGAGAGAACCTCATGGG CAGAATATTATAAAAAGAGTGTCATATTCCAGCATTTGAATTGGAAAGAGAAGAGTGAAGATCCAAGTTGCATTATTAATCTGCCCTGTGTTTTTTCCTT TTAACAATCAGTTTGAGCTGCTGCTGTTATGAGTTTCTCATCAAGATGAAAGCCCTAATATGTAAAGTCAAATCCGATTTAAATTTTGTGCTTTTATAGA AAGAAATTTCTTCATAGACGTGGTGATATATCATTTGTTGGACCTGCTAATAGTAGGTCAAAGGGGAGCACTCCTTGCCCCCTGTTCCTGGGTTTATGCA GTTTTCTTTTTAGAGTTTATATAGGGCAAGTGGTTCTTTTTCTCTGAATTACAGGATGGAAAAAGGTCATATCCTTTGTCAGGAAATATAAACTTGAAAG TATGTAGTCAGCTCTTGTAATACTCATATTTATGATTGTCCTATATGAAAAACAACTTCAGTTAAAACTATAATGTGTGATTCTGTATAACAAGGTGATG TCTGTTTCCCAGGGCTCAGACCTAATCCAGTTATAATAAAATCAATTAAATGAAATATTCTATAGAATCGATCTATGCCCTTGTTAATCTCCATCCATAT AGGAGTCACGTTCTTTAAGACAGATGGTGGTAGTTATTTTTGTGGCATGGTTAGATTTGACTGGTTTTGCAGAAGATTACAGTTATGTACTGCATAATGA CATATACAATAGTGGTCCCATAAAATTATAATGGAGCAGAAAATCTATTGCCTCATGATGTTTTAGCCGTTTTAATGTCATAGCCTAGTGCATTACTCAC GTGTCTGTGGAGATGCTGGTGTAAACAAACCTACTACACTGCCAGTTGTATAAAAGTACAGCACATTCAGTTATGTACAGTATGTAATACTGATAATGAC AATAAATGACACCGGTTTGTGTATTTACTTTTTATAA

refseq_B3GALNT1.F3 (forward primer)
refseq_B3GALNT1.R3 (reverse primer)
Single exon skipping

Frame 0

ASGRGPGAWSARARPCVRMRGGCVCAQGENAGH LPASELGARRAQEIACV*EVAALWEKLSRKKLPN HVFFSVFRVVHNRSECFN*AWNTENNKKLKL*F HLEFHSFLSSLDPVDLLALPAGCSITWCSPTTH PECKVLLLCCPGWSTVAGSQLTAISVSQV*AVLL PQPPK*VGLQNLRLACF*AAVDGLGSLDCPSE* DVTEIPQMEPPAAVTPEFLCDVVPQPSPLQCDR TRELDVLL*V*ADLQTRLSLHTSRAFKLLSSKSI SGHSGDLPPFRCESQAGH*SYLG*KKVLVGI*G SYIFLIRPRG*KGRQNVGIVLRG*TPSLW*HNP TRFFRHI**PDLENHYGIQVGN*VLPQCQVRNED RH*CFHQYWQFSEVSFKPKPLREVFHRLSSN** LFL*RILPKNPYFLPGVSFQGVPSILQWVGLYN VQRFGAKDL*NDGSRKTHQV*RCLCRDLFEFIKS EHSYSRRHKSFLSI*NPFGCLSTETCDCSPWLF FQGDHHFLAGHAKEHHMPLLTSHSTKSLEGQDT LWKVLNKVGTVENSWGGQCAGLH*TETHEKPRLE TGGLHL*FISQALQR*YVEELNIKELEVFAKEI NRTKQFGHVIL*TRIS*KGVTEL*AH*AVKTKQ CRVLFIEQCSHLKVLCISYVDYQFKNICSSVSKN FFTEVILNKILPVFGHL*STSRCCSISQLLLFK ITSTLCF*MF*RFQYKIKRIVNHSLHANIFQLL N*SVYY*YITPLM*SHRSLLHISNLLDFVKYFTV VI*RRIKARKSEVLSCF*KIQFLVFLEVT*FVS FFHLEIRNNVECKAVISFWKGL*RQKRRERTSW AEYYKKSVIFQHLNWKEKSEDPSCIINLPCVFSF *QSV*AAAVMSFSSR*KP*YVKSNPI*ILCFYR KKFLHRRGDISFVGPANSRSKGSTPCPLFLGLC SFLFRVYIGQVVLFL*ITGWKKVISFVRKYKLES M*SALVILIFMIVLYEKQLQLKL*CVILYNKVM SVSQGSDLIQL**NQLNEIFYRIDLCPC*SPSI *ESRSLRQMVVVIFVAWLDLTGFAEDYSYVLHND IYNSGPIKL*WSRKSIAS*CFSRFNVIA*CITH VSVEMLV*TNLLHCQLYKSTAHSVMYSM*Y*** Q*MTPVCVFTFY

Frame 1

LPAAALARGLRAPGRACGCGEAACVRRERTPAT FPLPSWVRAEHRRLPAFRRWLRCGKSYQGRNCQ TMSFFLFSE*FTTDLSVLIKHGIQKTTKNLSFNF IWNSTVFLAPWTRLTCWLFPLAALSRGALRLLT PSVKSCCSVAQAGVQWQDLSSLQSPSLRFKQFS CLSLPSRWDYRTFGSRASELLWMASALWTVLPSR MSLRSLKWSLLLLSLLSFFVMWYLSLPHYNVIE RVNWMYFYEYEPIYRQDFHFTLREHSNCSHQNP FLVILVTSHPSDVKARQAIRVTWGEKKSWWGYEV LTFFLLGQEAEKEDKMLALSLEDEHLLYGDIIR QDFLDTYNNLTLKTIMAFRWVTEFCPNAKYVMK TDTDVFINTGNLVKYLLNLNHSEKFFTGYPLIDN YSYRGFYQKTHISYQEYPFKVFPPYCSGLGYIM SRDLVPRIYEMMGHVKPIKFEDVYVGICLNLLK VNIHIPEDTNLFFLYRIHLDVCQLRRVIAAHGFS SKEIITFWQVMLRNTTCHY*LHILQKA*KDRIL CGKC*IK*VLWKIHGEVSVLAYTELKLMKNPDW RLEGYTCDLLVRPFKDDMWRN*I*RNWRFLLKKL IGPNNLDMSFCRLEFLKRVLLSYKLTRL*KQNN VEFYLLNNVVT*RFCVYLMWITNLKIYVVLCQK TSSLKLY*TKFYLFLVIYKVLQDVAVFHSYYYLK LLQLCVFKCFDDFNTR*KG**IILYMQTFSSYL TDQFIIDTSLH*CKVIGHYCISVISWTLLNILL W*YREELKQENLKYCLVFKKYSS*CF*KSLNLSH FSTWKLGIM*NARQ*FPFGKDSEGRKEGREPHG QNIIKRVSYSSI*IGKRRVKIQVALLICPVFFP FNNQFELLLL*VSHQDESPNM*SQIRFKFCAFIE RNFFIDVVIYHLLDLLIVGQRGALLAPCSWVYA VFFLEFI*GKWFFFSELQDGKRSYPLSGNINLK VCSQLL*YSYL*LSYMKNNFS*NYNV*FCITR*C LFPRAQT*SSYNKIN*MKYSIESIYALVNLHPY RSHVL*DRWW*LFLWHG*I*LVLQKITVMYCIM TYTIVVP*NYNGAENLLPHDVLAVLMS*PSALLT CLWRCWCKQTYYTASCIKVQHIQLCTVCNTDND NK*HRFVYLLFI

Frame 2

FRPRPWRVVCARPAVRADAGRLRVCAGRERRPP SRFRAGCAPSTGDCLRLGGGCVVGKAIKEEIAK PCLFFCFQSSSQQI*VF*LSMEYRKQQKT*ALI SSGIPQFS*LPGPG*PVGSSRWLLYHVVLSDYSP RV*SLAALLPRLEYSGRISAHCNLRLSGLSSSP ASASQVGGTTEPSARVLLSCCGWPRLSGLSFRV GCH*DPSNGASCCCHS*VSL*CGTSAFPTTM**N A*TGCTSMSMSRFTDKTFTSHFESIQTALIKIH FWSFW*PPTLQM*KPGRPLELLGVKKSLGGDMR FLHFSY*AKRLKRKTKCWHCP*RMNTFFMVT*SD KIF*THIIT*P*KPLWHSGG*LSFAPMPST**R QTLMFSSILAI**SIF*T*TTQRSFSQVIL*LI IIPIEDFTKKPIFLTRSILSRCSLHTAVGWVI*C PEIWCQGSMK*WVT*NPSSLKMFMSGSV*IY*K *TFIFQKTQIFSFYIESIWMSVN*DV*LQPMAF LPRRSSLFGRSC*GTPHAIINFTFYKKPRRTGYF VESVK*SRYCGKFMGRSVCWLTLN*NS*KTQTG DWRVTLVIY*SGPSKMICGGIKYKGIGGFC*RN **DQTIWTCHSVD*NFLKGCY*VISSLGCKNKTM *SFIY*TM*SLEGFVYILCGLPI*KYM*FCVKK LLH*SYTEQNFTCFWSFIKYFKMLQYFTVIII* NYFNFVFLNVLTISIQDKKDSESFFTCKHFPVT* LISLLLIHHSINVKS*VIIAYQ*SLGLC*IFYC GNIEKN*SKKI*SIVLFLKNTVPSVFRSHLICL IFPPGN*E*CRMQGSNFLLERTLKAEKKGENLMG RIL*KECHIPAFELEREE*RSKLHY*SALCFFL LTISLSCCCYEFLIKMKALICKVKSDLNFVLL* KEISS*TW*YIICWTC***VKGEHSLPPVPGFMQ FSF*SLYRASGSFSLNYRMEKGHILCQEI*T*K YVVSSCNTHIYDCPI*KTTSVKTIMCDSV*QGD VCFPGLRPNPVIIKSIK*NIL*NRSMPLLISIHI GVTFFKTDGGSYFCGMVRFDWFCRRLQLCTA** HIQ*WSHKIIMEQKIYCLMMF*PF*CHSLVHYS RVCGDAGVNKPTTLPVV*KYSTFSYVQYVILIMT INDTGLCIYFL*

Sequence NM_001038628

GCTTCCGGCCGCGGCCCTGGCGCGTGGTCTGCGCGCGCCCGGCCGTGCGTGCGGATGCGGGGAGGCTGCGTGTGTGCGCAGGGAGAGAACGCCGGCCACC TTCCCGCTTCCGAGCTGGGTGCGCGCCGAGCACAGGAGGGTGCAATACCTGCCTTGCGTCGCCCGTGCCGCTCCAGCCACCGTCAAATCCGGGTAGCGAC CCGGGGCGTTTGCAGCGGTGCCGAGGAAGAGGACGGGAACGGTGTTACGATTGCCTGCGTTTAGGAGGTGGCTGCGTTGTGGGAAAAGCTATCAAGGAAG AAATTGCCAAACCATGTCTTTTTTTCTGTTTTCAGAGTAGTTCACAACAGATCTGAGTGTTTTAATTAAGCATGGAATACAGAAAACAACAAAAAACTTA AGCTTTAATTTCATCTGGAATTCCACAGAGACAAGGCCACTTTATGTTTCCCAGGCTGGACTGGAACCCCTGGGCTCAAATGATCCTCCTACCTAAGCCT GCAGAGTAGCGAGGACTTCAGTTTTCTTAGCTCCCTGGACCCGGTTGACCTGTTGGCTCTTCCCGCTGGCTGCTCTATCACGTGGTGCTCTCCGACTACT CACCCCGAGTGTAAAGTCTTGCTGCTCTGTTGCCCAGGCTGGAGTACAGTGGCAGGATCTCAGCTCACTGCAATCTCCGTCTCTCAGGTTTAAGCAGTTC TCCTGCCTCAGCCTCCCAAGTAGGTGGGACTACAGAACCTTCGGCTCGCGTGCTTCTGAGCTGCTGTGGATGGCCTCGGCTCTCTGGACTGTCCTTCCGA GTAGGATGTCACTGAGATCCCTCAAATGGAGCCTCCTGCTGCTGTCACTCCTGAGTTTCTTTGTGATGTGGTACCTCAGCCTTCCCCACTACAATGTGAT AGAACGCGTGAACTGGATGTACTTCTATGAGTATGAGCCGATTTACAGACAAGACTTTCACTTCACACTTCGAGAGCATTCAAACTGCTCTCATCAAAAT CCATTTCTGGTCATTCTGGTGACCTCCCACCCTTCAGATGTGAAAGCCAGGCAGGCCATTAGAGTTACTTGGGGTGAAAAAAAGTCTTGGTGGGGATATG AGGTTCTTACATTTTTCTTATTAGGCCAAGAGGCTGAAAAGGAAGACAAAATGTTGGCATTGTCCTTAGAGGATGAACACCTTCTTTATGGTGACATAAT CCGACAAGATTTTTTAGACACATATAATAACCTGACCTTGAAAACCATTATGGCATTCAGGTGGGTAACTGAGTTTTGCCCCAATGCCAAGTACGTAATG AAGACAGACACTGATGTTTTCATCAATACTGGCAATTTAGTGAAGTATCTTTTAAACCTAAACCACTCAGAGAAGTTTTTCACAGGTTATCCTCTAATTG ATAATTATTCCTATAGAGGATTTTACCAAAAAACCCATATTTCTTACCAGGAGTATCCTTTCAAGGTGTTCCCTCCATACTGCAGTGGGTTGGGTTATAT AATGTCCAGAGATTTGGTGCCAAGGATCTATGAAATGATGGGTCACGTAAAACCCATCAAGTTTGAAGATGTTTATGTCGGGATCTGTTTGAATTTATTA AAAGTGAACATTCATATTCCAGAAGACACAAATCTTTTCTTTCTATATAGAATCCATTTGGATGTCTGTCAACTGAGACGTGTGATTGCAGCCCATGGCT TTTCTTCCAAGGAGATCATCACTTTTTGGCAGGTCATGCTAAGGAACACCACATGCCATTATTAACTTCACATTCTACAAAAAGCCTAGAAGGACAGGAT ACTTTGTGGAAAGTGTTAAATAAAGTAGGTACTGTGGAAAATTCATGGGGAGGTCAGTGTGCTGGCTTACACTGAACTGAAACTCATGAAAAACCCAGAC TGGAGACTGGAGGGTTACACTTGTGATTTATTAGTCAGGCCCTTCAAAGATGATATGTGGAGGAATTAAATATAAAGGAATTGGAGGTTTTTGCTAAAGA AATTAATAGGACCAAACAATTTGGACATGTCATTCTGTAGACTAGAATTTCTTAAAAGGGTGTTACTGAGTTATAAGCTCACTAGGCTGTAAAAACAAAA CAATGTAGAGTTTTATTTATTGAACAATGTAGTCACTTGAAGGTTTTGTGTATATCTTATGTGGATTACCAATTTAAAAATATATGTAGTTCTGTGTCAA AAAACTTCTTCACTGAAGTTATACTGAACAAAATTTTACCTGTTTTTGGTCATTTATAAAGTACTTCAAGATGTTGCAGTATTTCACAGTTATTATTATT TAAAATTACTTCAACTTTGTGTTTTTAAATGTTTTGACGATTTCAATACAAGATAAAAAGGATAGTGAATCATTCTTTACATGCAAACATTTTCCAGTTA CTTAACTGATCAGTTTATTATTGATACATCACTCCATTAATGTAAAGTCATAGGTCATTATTGCATATCAGTAATCTCTTGGACTTTGTTAAATATTTTA CTGTGGTAATATAGAGAAGAATTAAAGCAAGAAAATCTGAAGTATTGTCTTGTTTTTAAAAAATACAGTTCCTAGTGTTTTTAGAAGTCACTTAATTTGT CTCATTTTTCCACCTGGAAATTAGGAATAATGTAGAATGCAAGGCAGTAATTTCCTTTTGGAAAGGACTCTGAAGGCAGAAAAGAAGGGAGAGAACCTCA TGGGCAGAATATTATAAAAAGAGTGTCATATTCCAGCATTTGAATTGGAAAGAGAAGAGTGAAGATCCAAGTTGCATTATTAATCTGCCCTGTGTTTTTT CCTTTTAACAATCAGTTTGAGCTGCTGCTGTTATGAGTTTCTCATCAAGATGAAAGCCCTAATATGTAAAGTCAAATCCGATTTAAATTTTGTGCTTTTA TAGAAAGAAATTTCTTCATAGACGTGGTGATATATCATTTGTTGGACCTGCTAATAGTAGGTCAAAGGGGAGCACTCCTTGCCCCCTGTTCCTGGGTTTA TGCAGTTTTCTTTTTAGAGTTTATATAGGGCAAGTGGTTCTTTTTCTCTGAATTACAGGATGGAAAAAGGTCATATCCTTTGTCAGGAAATATAAACTTG AAAGTATGTAGTCAGCTCTTGTAATACTCATATTTATGATTGTCCTATATGAAAAACAACTTCAGTTAAAACTATAATGTGTGATTCTGTATAACAAGGT GATGTCTGTTTCCCAGGGCTCAGACCTAATCCAGTTATAATAAAATCAATTAAATGAAATATTCTATAGAATCGATCTATGCCCTTGTTAATCTCCATCC ATATAGGAGTCACGTTCTTTAAGACAGATGGTGGTAGTTATTTTTGTGGCATGGTTAGATTTGACTGGTTTTGCAGAAGATTACAGTTATGTACTGCATA ATGACATATACAATAGTGGTCCCATAAAATTATAATGGAGCAGAAAATCTATTGCCTCATGATGTTTTAGCCGTTTTAATGTCATAGCCTAGTGCATTAC TCACGTGTCTGTGGAGATGCTGGTGTAAACAAACCTACTACACTGCCAGTTGTATAAAAGTACAGCACATTCAGTTATGTACAGTATGTAATACTGATAA TGACAATAAATGACACCGGTTTGTGTATTTACTTTTTATAA

refseq_B3GALNT1.F3 (forward primer)
refseq_B3GALNT1.R3 (reverse primer)
Single exon skipping

Frame 0

ASGRGPGAWSARARPCVRMRGGCVCAQGENAGH LPASELGARRAQEGAIPALRRPCRSSHRQIRVAT RGVCSGAEEEDGNGVTIACV*EVAALWEKLSRK KLPNHVFFSVFRVVHNRSECFN*AWNTENNKKL KL*FHLEFHRDKATLCFPGWTGTPGLK*SSYLSL QSSEDFSFLSSLDPVDLLALPAGCSITWCSPTT HPECKVLLLCCPGWSTVAGSQLTAISVSQV*AV LLPQPPK*VGLQNLRLACF*AAVDGLGSLDCPSE *DVTEIPQMEPPAAVTPEFLCDVVPQPSPLQCD RTRELDVLL*V*ADLQTRLSLHTSRAFKLLSSK SISGHSGDLPPFRCESQAGH*SYLG*KKVLVGI* GSYIFLIRPRG*KGRQNVGIVLRG*TPSLW*HN PTRFFRHI**PDLENHYGIQVGN*VLPQCQVRN EDRH*CFHQYWQFSEVSFKPKPLREVFHRLSSN* *LFL*RILPKNPYFLPGVSFQGVPSILQWVGLY NVQRFGAKDL*NDGSRKTHQV*RCLCRDLFEFI KSEHSYSRRHKSFLSI*NPFGCLSTETCDCSPWL FFQGDHHFLAGHAKEHHMPLLTSHSTKSLEGQD TLWKVLNKVGTVENSWGGQCAGLH*TETHEKPR LETGGLHL*FISQALQR*YVEELNIKELEVFAKE INRTKQFGHVIL*TRIS*KGVTEL*AH*AVKTK QCRVLFIEQCSHLKVLCISYVDYQFKNICSSVS KNFFTEVILNKILPVFGHL*STSRCCSISQLLLF KITSTLCF*MF*RFQYKIKRIVNHSLHANIFQL LN*SVYY*YITPLM*SHRSLLHISNLLDFVKYF TVVI*RRIKARKSEVLSCF*KIQFLVFLEVT*FV SFFHLEIRNNVECKAVISFWKGL*RQKRRERTS WAEYYKKSVIFQHLNWKEKSEDPSCIINLPCVF SF*QSV*AAAVMSFSSR*KP*YVKSNPI*ILCFY RKKFLHRRGDISFVGPANSRSKGSTPCPLFLGL CSFLFRVYIGQVVLFL*ITGWKKVISFVRKYKL ESM*SALVILIFMIVLYEKQLQLKL*CVILYNKV MSVSQGSDLIQL**NQLNEIFYRIDLCPC*SPS I*ESRSLRQMVVVIFVAWLDLTGFAEDYSYVLH NDIYNSGPIKL*WSRKSIAS*CFSRFNVIA*CIT HVSVEMLV*TNLLHCQLYKSTAHSVMYSM*Y** *Q*MTPVCVFTFY

Frame 1

LPAAALARGLRAPGRACGCGEAACVRRERTPAT FPLPSWVRAEHRRVQYLPCVARAAPATVKSG*R PGAFAAVPRKRTGTVLRLPAFRRWLRCGKSYQGR NCQTMSFFLFSE*FTTDLSVLIKHGIQKTTKNL SFNFIWNSTETRPLYVSQAGLEPLGSNDPPT*A CRVARTSVFLAPWTRLTCWLFPLAALSRGALRLL TPSVKSCCSVAQAGVQWQDLSSLQSPSLRFKQF SCLSLPSRWDYRTFGSRASELLWMASALWTVLP SRMSLRSLKWSLLLLSLLSFFVMWYLSLPHYNVI ERVNWMYFYEYEPIYRQDFHFTLREHSNCSHQN PFLVILVTSHPSDVKARQAIRVTWGEKKSWWGY EVLTFFLLGQEAEKEDKMLALSLEDEHLLYGDII RQDFLDTYNNLTLKTIMAFRWVTEFCPNAKYVM KTDTDVFINTGNLVKYLLNLNHSEKFFTGYPLI DNYSYRGFYQKTHISYQEYPFKVFPPYCSGLGYI MSRDLVPRIYEMMGHVKPIKFEDVYVGICLNLL KVNIHIPEDTNLFFLYRIHLDVCQLRRVIAAHG FSSKEIITFWQVMLRNTTCHY*LHILQKA*KDRI LCGKC*IK*VLWKIHGEVSVLAYTELKLMKNPD WRLEGYTCDLLVRPFKDDMWRN*I*RNWRFLLK KLIGPNNLDMSFCRLEFLKRVLLSYKLTRL*KQN NVEFYLLNNVVT*RFCVYLMWITNLKIYVVLCQ KTSSLKLY*TKFYLFLVIYKVLQDVAVFHSYYY LKLLQLCVFKCFDDFNTR*KG**IILYMQTFSSY LTDQFIIDTSLH*CKVIGHYCISVISWTLLNIL LW*YREELKQENLKYCLVFKKYSS*CF*KSLNL SHFSTWKLGIM*NARQ*FPFGKDSEGRKEGREPH GQNIIKRVSYSSI*IGKRRVKIQVALLICPVFF PFNNQFELLLL*VSHQDESPNM*SQIRFKFCAF IERNFFIDVVIYHLLDLLIVGQRGALLAPCSWVY AVFFLEFI*GKWFFFSELQDGKRSYPLSGNINL KVCSQLL*YSYL*LSYMKNNFS*NYNV*FCITR *CLFPRAQT*SSYNKIN*MKYSIESIYALVNLHP YRSHVL*DRWW*LFLWHG*I*LVLQKITVMYCI MTYTIVVP*NYNGAENLLPHDVLAVLMS*PSAL LTCLWRCWCKQTYYTASCIKVQHIQLCTVCNTDN DNK*HRFVYLLFI

Frame 2

FRPRPWRVVCARPAVRADAGRLRVCAGRERRPP SRFRAGCAPSTGGCNTCLASPVPLQPPSNPGSD PGRLQRCRGRGRERCYDCLRLGGGCVVGKAIKE EIAKPCLFFCFQSSSQQI*VF*LSMEYRKQQKT* ALISSGIPQRQGHFMFPRLDWNPWAQMILLPKP AE*RGLQFS*LPGPG*PVGSSRWLLYHVVLSDY SPRV*SLAALLPRLEYSGRISAHCNLRLSGLSSS PASASQVGGTTEPSARVLLSCCGWPRLSGLSFR VGCH*DPSNGASCCCHS*VSL*CGTSAFPTTM* *NA*TGCTSMSMSRFTDKTFTSHFESIQTALIKI HFWSFW*PPTLQM*KPGRPLELLGVKKSLGGDM RFLHFSY*AKRLKRKTKCWHCP*RMNTFFMVT* SDKIF*THIIT*P*KPLWHSGG*LSFAPMPST** RQTLMFSSILAI**SIF*T*TTQRSFSQVIL*L IIIPIEDFTKKPIFLTRSILSRCSLHTAVGWVI *CPEIWCQGSMK*WVT*NPSSLKMFMSGSV*IY* K*TFIFQKTQIFSFYIESIWMSVN*DV*LQPMA FLPRRSSLFGRSC*GTPHAIINFTFYKKPRRTG YFVESVK*SRYCGKFMGRSVCWLTLN*NS*KTQT GDWRVTLVIY*SGPSKMICGGIKYKGIGGFC*R N**DQTIWTCHSVD*NFLKGCY*VISSLGCKNK TM*SFIY*TM*SLEGFVYILCGLPI*KYM*FCVK KLLH*SYTEQNFTCFWSFIKYFKMLQYFTVIII *NYFNFVFLNVLTISIQDKKDSESFFTCKHFPV T*LISLLLIHHSINVKS*VIIAYQ*SLGLC*IFY CGNIEKN*SKKI*SIVLFLKNTVPSVFRSHLIC LIFPPGN*E*CRMQGSNFLLERTLKAEKKGENL MGRIL*KECHIPAFELEREE*RSKLHY*SALCFF LLTISLSCCCYEFLIKMKALICKVKSDLNFVLL *KEISS*TW*YIICWTC***VKGEHSLPPVPGF MQFSF*SLYRASGSFSLNYRMEKGHILCQEI*T* KYVVSSCNTHIYDCPI*KTTSVKTIMCDSV*QG DVCFPGLRPNPVIIKSIK*NIL*NRSMPLLISI HIGVTFFKTDGGSYFCGMVRFDWFCRRLQLCTA* *HIQ*WSHKIIMEQKIYCLMMF*PF*CHSLVHY SRVCGDAGVNKPTTLPVV*KYSTFSYVQYVILI MTINDTGLCIYFL*