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Sequences

Sequence NM_198337

GGGCCCCGCGGCCCGCCATATAAACCCGCGCGCCCGCCAGGCGCTGCGGCCGTCCCGGGCCGTGACTCCTCCTTTCCCCCGCCCCGCCTCCGTTCGGAGA GCCGGCGGGCGGGCGCCTCTCGGCCAGGAAGCGCCTCTTGGACGCGTGTGACCGATGCCCAGATTGCACGACCACTTCTGGAGCTGCTCCTGTGCGCACA GCGCGAGGCGCCGAGGCCCCCCGCGAGCCAGCGCCGCGGGGCTGGCGGCCAAGGTTGGGGAGATGATCAACGTTTCCGTGTCCGGGCCCTCCCTGCTGGC GGCCCACGGTGCCCCGGACGCTGACCCCGCGCCCAGGGGCCGCAGTGCTGCGATGAGCGGCCCCGAGCCCGGCAGCCCCTACCCCAACACCTGGCATCAT CGCCTGTTGCAGAGGAGCCTCGTGCTCTTCTCGGTTGGGGTGGTCCTAGCCCTGGTGCTCAACCTGCTGCAGATCCAGAGGAATGTCACTCTCTTCCCCG AGGAGGTGATCGCCACCATCTTTTCCTCCGCCTGGTGGGTCCCTCCCTGCTGCGGGACAGCAGCTGGTATACATCCCCAGATTTCCTCTATATTCGTTCT TGGCTCCCTTGTATATTTTTCTCAGGAGGCGTCACGGTGGGGAACATAGGACGACAGTTAGCTATGGGTGTTCCTGAAAAGCCCCATAGTGATTGAGTCT TCAAAACCACCGATTCTGAGAGCAAGGAAGATTTTGGAAGAAAATCTGACTGTGGATTATGACAAAGATTATCTTTTTTCTTAAGTAATCTATTTAGATC GGGCTGACTGTACAAATGACTCCTGGAAAAAACTCTTCACCTAGTCTAGAATAGGGAGGTGGAGAATGATGACTTACCCTGAAGTCTTCCCTTGACTGCC CGCACTGGCGCCTGTCTGTGCCCTGGAGCATTCTGCCCAGGCTACGTGGGTTCAGGCAGGTGGCAGCTTCCCAAGTATTCGATTTCATTCATGTGATTAA AACAAGTTGCCATATTTCAAAGCCTTGAACTAAGACTCAATTACCAACCCGCAGTTTTGTGTCAGTGCCCAAAGGAGGTAGGTTGATGGTGCTTAACAAA CATGAAGTATGGTGTAATAGGAATAATATTTATCCAAAAGATTTTTAAAAATAGGGCTGTGTTTAAAAAAAAAAAACAAAACAAGAAAAGCAGCAGTGAT TATAGAGAGGTCACACTCTAAGTGGGGTCGCGGCGTGGCCACGCTTCACGGTCACGCTCGTCCGTCCTGCAGTGGCGTGTTTACATGGTCACACGTGTGT GTATCACCAGTGGGTCAACTGCTTGTCATTCCTCCCGTGGCAGTTTGTGTAGACAATCTTACTGAGCAAAAGGCAATGAAAAGTCTTGGTTCCCACACTG CGATATATTGGAATTTTCACCTCAGTTTATGAAGTTTATTTCGAAATCCATAGTCATCTAAGAATGAATACCTGTCTGCCATGTATTTCAATCTTAGTGA GCCAAAATTGTTTGTTTGTTACTACAGAATAGAGATGACTGTTTTTTGCCACAGCCCTATGGAATTTGCAATCTGTGATTGCCTTGTAAAAAGGAGAGTG CATATGGCACTGCATTAAACGTGTGGTGTTTCTAGTCAATGATATTGGTGAGCACAATGTATTCATTTAATGGCATAGACCATACCAGACCTAATTTGCA AGTATTGGGTCTTAAACTTCAAGTGCAATGTATATGAAAACCAATCTGAGCCTTGTATCTCTTAAATATTTATTTTTTTTAACGTGTGAGATGTTCGAGA GAAGGTTCTCCATTCATTTCAGTGCTGCCTGGAGGAAACTCGGCAATGATTTCTTTCAGTTGTGAAGTTCCTTTCGTGTTACACCCTCCACTGAACCCTC AACCTTCGAAATACTCCAGTTTTGTGGGTTTGGTCATTTTTACTTATAAATTTACCTTTTTGTATTTTGCAATTTACATGTGTTTGGTTTGTTTTAAATT CTGTGAAAGTGGCTTGATTAAAAGACTCCTTTTAAATGGAAGCCACCAGTCAGCAGAATGGAAGCTTAGAGGAACTTGCCTGTGAGCGCTGGTCTTTGTG TTTGGTTTTGTGATGTAACGATCTTTGCTGGGGTTTTTTGCTTTGTTTTGAGGGAAATGTCTTGGAGTAAATTTTAAGTTCCTGGAGTTAATTTGTTTTA CAGGAATTTTGTTTTTTAAAAAAATAGGATCATTCTGAACTTTGGAATGACCCCCTTATATATTTTCTGAAAATGAAAACAGTTACATGAAAAAAATTTC CAATGAAGATGTCAGCATTTTATGAAAAACCAGAAGTTATTAGATGAAAGCAGCGAGTGAATCTTTAAAACAGACTTGATCACGCACACACAATAAGTCT TTCTCTCCGAAACCGGAAGTAAATCTATATCTGTTAGAAATAATGTAGCCAAAAGAATGTAAATTTGAGGATTTTTTTGCCAATAGTTTATAGAAAATAT ATGAACCAAAGTGATTTGAGTTTGTAAAAATGTAAAATAGTATGAACAAAATTTGCACTCTACCAGATTTGAACATCTAGTGAGGTTCACATTCATACTA AGTTTTCAACATTGTGTTCTTTTTGCATTCATTTTTTACTTTTATTAAAGGTTCAAAACCAA

refseq_INSIG1.F1 (forward primer)
refseq_INSIG1.R1 (reverse primer)
Multiple exon skipping

Frame 0

GPRGPPYKPARPPGAAAVPGRDSSFPPPRLRSE SRRAGASRPGSASWTRVTDAQIARPLLELLLCAQ REAPRPPASQRRGAGGQGWGDDQRFRVRALPAG GPRCPGR*PRAQGPQCCDERPRARQPLPQHLAS SPVAEEPRALLGWGGPSPGAQPAADPEECHSLPR GGDRHHLFLRLVGPSLLRDSSWYTSPDFLYIRS WLPCIFFSGGVTVGNIGRQLAMGVPEKPHSD*V FKTTDSESKEDFGRKSDCGL*QRLSFFLSNLFRS G*LYK*LLEKTLHLV*NREVENDDLP*SLPLTA RTGACLCPGAFCPGYVGSGRWQLPKYSISFM*L KQVAIFQSLELRLNYQPAVLCQCPKEVG*WCLTN MKYGVIGIIFIQKIFKNRAVFKKKKQNKKSSSD YREVTL*VGSRRGHASRSRSSVLQWRVYMVTRV CITSGSTACHSSRGSLCRQSY*AKGNEKSWFPHC DILEFSPQFMKFISKSIVI*E*IPVCHVFQS** AKIVCLLLQNRDDCFLPQPYGICNL*LPCKKES AYGTALNVWCF*SMILVSTMYSFNGIDHTRPNLQ VLGLKLQVQCI*KPI*ALYLLNIYFF*RVRCSR EGSPFISVLPGGNSAMISFSCEVPFVLHPPLNP QPSKYSSFVGLVIFTYKFTFLYFAIYMCLVCFKF CESGLIKRLLLNGSHQSAEWKLRGTCL*ALVFV FGFVM*RSLLGFFALF*GKCLGVNFKFLELICF TGILFFKKIGSF*TLE*PPYIFSENENSYMKKIS NEDVSIL*KTRSY*MKAASESLKQT*SRTHNKS FSPKPEVNLYLLEIM*PKECKFEDFFANSL*KI YEPK*FEFVKM*NSMNKICTLPDLNI**GSHSY* VFNIVFFLHSFFTFIKGSKP

Frame 1

GPAARHINPRARQALRPSRAVTPPFPRPASVRR AGGRAPLGQEAPLGRV*PMPRLHDHFWSCSCAH SARRRGPPRASAAGLAAKVGEMINVSVSGPSLLA AHGAPDADPAPRGRSAAMSGPEPGSPYPNTWHH RLLQRSLVLFSVGVVLALVLNLLQIQRNVTLFP EEVIATIFSSAWWVPPCCGTAAGIHPQISSIFVL GSLVYFSQEASRWGT*DDS*LWVFLKSPIVIES SKPPILRARKILEENLTVDYDKDYLFS*VIYLD RADCTNDSWKKLFT*SRIGRWRMMTYPEVFP*LP ALAPVCALEHSAQATWVQAGGSFPSIRFHSCD* NKLPYFKALN*DSITNPQFCVSAQRR*VDGA*Q T*SMV**E*YLSKRFLKIGLCLKKKNKTRKAAVI IERSHSKWGRGVATLHGHARPSCSGVFTWSHVC VSPVGQLLVIPPVAVCVDNLTEQKAMKSLGSHT AIYWNFHLSL*SLFRNP*SSKNEYLSAMYFNLSE PKLFVCYYRIEMTVFCHSPMEFAICDCLVKRRV HMALH*TCGVSSQ*YW*AQCIHLMA*TIPDLIC KYWVLNFKCNVYENQSEPCIS*IFIFFNV*DVRE KVLHSFQCCLEETRQ*FLSVVKFLSCYTLH*TL NLRNTPVLWVWSFLLINLPFCILQFTCVWFVLN SVKVA*LKDSF*MEATSQQNGSLEELACERWSLC LVL*CNDLCWGFLLCFEGNVLE*ILSSWS*FVL QEFCFLKK*DHSELWNDPLIYFLKMKTVT*KKF PMKMSAFYEKPEVIR*KQRVNL*NRLDHAHTISL SLRNRK*IYIC*K*CSQKNVNLRIFLPIVYRKY MNQSDLSL*KCKIV*TKFALYQI*TSSEVHIHT KFSTLCSFCIHFLLLLKVQNQ

Frame 2

APRPAI*TRAPARRCGRPGP*LLLSPAPPPFGE PAGGRLSARKRLLDACDRCPDCTTTSGAAPVRT ARGAEAPREPAPRGWRPRLGR*STFPCPGPPCW RPTVPRTLTPRPGAAVLR*AAPSPAAPTPTPGII ACCRGASCSSRLGWS*PWCSTCCRSRGMSLSSP RR*SPPSFPPPGGSLPAAGQQLVYIPRFPLYSF LAPLYIFLRRRHGGEHRTTVSYGCS*KAP**LSL QNHRF*EQGRFWKKI*LWIMTKIIFFLK*SI*I GLTVQMTPGKNSSPSLE*GGGE**LTLKSSLDC PHWRLSVPWSILPRLRGFRQVAASQVFDFIHVIK TSCHISKP*TKTQLPTRSFVSVPKGGRLMVLNK HEVWCNRNNIYPKDF*K*GCV*KKKTKQEKQQ* L*RGHTLSGVAAWPRFTVTLVRPAVACLHGHTCV YHQWVNCLSFLPWQFV*TILLSKRQ*KVLVPTL RYIGIFTSVYEVYFEIHSHLRMNTCLPCISILV SQNCLFVTTE*R*LFFATALWNLQSVIAL*KGEC IWHCIKRVVFLVNDIGEHNVFI*WHRPYQT*FA SIGS*TSSAMYMKTNLSLVSLKYLFFLTCEMFE RRFSIHFSAAWRKLGNDFFQL*SSFRVTPSTEPS TFEILQFCGFGHFYL*IYLFVFCNLHVFGLF*I L*KWLD*KTPFKWKPPVSRMEA*RNLPVSAGLC VWFCDVTIFAGVFCFVLREMSWSKF*VPGVNLFY RNFVF*KNRIILNFGMTPLYIF*K*KQLHEKNF Q*RCQHFMKNQKLLDESSE*IFKTDLITHTQ*V FLSETGSKSISVRNNVAKRM*I*GFFCQ*FIENI *TKVI*VCKNVK*YEQNLHSTRFEHLVRFTFIL SFQHCVLFAFIFYFY*RFKT

Sequence NM_198336

GGGCCCCGCGGCCCGCCATATAAACCCGCGCGCCCGCCAGGCGCTGCGGCCGTCCCGGGCCGTGACTCCTCCTTTCCCCCGCCCCGCCTCCGTTCGGAGA GCCGGCGGGCGGGCGCCTCTCGGCCAGGAAGCGCCTCTTGGACGCGTGTGACCGATGCCCAGATTGCACGACCACTTCTGGAGCTGCTCCTGTGCGCACA GCGCGAGGCGCCGAGGCCCCCCGCGAGCCAGCGCCGCGGGGCTGGCGGCCAAGGTTGGGGAGATGATCAACGTTTCCGTGTCCGGGCCCTCCCTGCTGGC GGCCCACGGTGCCCCGGACGCTGACCCCGCGCCCAGGGGCCGCAGTGCTGCGATGAGCGGCCCCGAGCCCGGCAGCCCCTACCCCAACACCTGGCATCAT CGCCTGTTGCAGAGGAGCCTCGTGCTCTTCTCGGTTGGGGTGGTCCTAGCCCTGGTGCTCAACCTGCTGCAGATCCAGAGGAATGTCACTCTCTTCCCCG AGGAGGTGATCGCCACCATCTTTTCCTCCGCCTGGTGGGTCCCTCCCTGCTGCGGGACAGCAGCTGCTGTTGTTGGCCTACTGTACCCCTGTATCGACAG TCACCTCGGAGAACCCCACAAATTTAAGAGAGAATGGGCCAGTGTCATGCGCTGCATAGCAGTTTTTGTTGGCATTAACCACGCCAGTGCTAAATTGGAT TTTGCCAATAATGTCCAGCTGTCCTTGACTTTAGCAGCCCTATCTTTGGGCCTTTGGTGGACATTTGATCGTTCCAGAAGTGGCCTTGGGCTGGGGATCA CCATAGCTTTTCTAGCTACGCTGATCACGCAGTTTCTCGTGTATAATGGTGTCTATCAGTATACATCCCCAGATTTCCTCTATATTCGTTCTTGGCTCCC TTGTATATTTTTCTCAGGAGGCGTCACGGTGGGGAACATAGGACGACAGTTAGCTATGCTGATACCCTTCTGTGAGGAGTTGAATTTGAAGACCACTTGG CTGTTTCACAAAACCAGAAGTAATTACAGGGTGTTCCTGAAAAGCCCCATAGTGATTGAGTCTTCAAAACCACCGATTCTGAGAGCAAGGAAGATTTTGG AAGAAAATCTGACTGTGGATTATGACAAAGATTATCTTTTTTCTTAAGTAATCTATTTAGATCGGGCTGACTGTACAAATGACTCCTGGAAAAAACTCTT CACCTAGTCTAGAATAGGGAGGTGGAGAATGATGACTTACCCTGAAGTCTTCCCTTGACTGCCCGCACTGGCGCCTGTCTGTGCCCTGGAGCATTCTGCC CAGGCTACGTGGGTTCAGGCAGGTGGCAGCTTCCCAAGTATTCGATTTCATTCATGTGATTAAAACAAGTTGCCATATTTCAAAGCCTTGAACTAAGACT CAATTACCAACCCGCAGTTTTGTGTCAGTGCCCAAAGGAGGTAGGTTGATGGTGCTTAACAAACATGAAGTATGGTGTAATAGGAATAATATTTATCCAA AAGATTTTTAAAAATAGGGCTGTGTTTAAAAAAAAAAAACAAAACAAGAAAAGCAGCAGTGATTATAGAGAGGTCACACTCTAAGTGGGGTCGCGGCGTG GCCACGCTTCACGGTCACGCTCGTCCGTCCTGCAGTGGCGTGTTTACATGGTCACACGTGTGTGTATCACCAGTGGGTCAACTGCTTGTCATTCCTCCCG TGGCAGTTTGTGTAGACAATCTTACTGAGCAAAAGGCAATGAAAAGTCTTGGTTCCCACACTGCGATATATTGGAATTTTCACCTCAGTTTATGAAGTTT ATTTCGAAATCCATAGTCATCTAAGAATGAATACCTGTCTGCCATGTATTTCAATCTTAGTGAGCCAAAATTGTTTGTTTGTTACTACAGAATAGAGATG ACTGTTTTTTGCCACAGCCCTATGGAATTTGCAATCTGTGATTGCCTTGTAAAAAGGAGAGTGCATATGGCACTGCATTAAACGTGTGGTGTTTCTAGTC AATGATATTGGTGAGCACAATGTATTCATTTAATGGCATAGACCATACCAGACCTAATTTGCAAGTATTGGGTCTTAAACTTCAAGTGCAATGTATATGA AAACCAATCTGAGCCTTGTATCTCTTAAATATTTATTTTTTTTAACGTGTGAGATGTTCGAGAGAAGGTTCTCCATTCATTTCAGTGCTGCCTGGAGGAA ACTCGGCAATGATTTCTTTCAGTTGTGAAGTTCCTTTCGTGTTACACCCTCCACTGAACCCTCAACCTTCGAAATACTCCAGTTTTGTGGGTTTGGTCAT TTTTACTTATAAATTTACCTTTTTGTATTTTGCAATTTACATGTGTTTGGTTTGTTTTAAATTCTGTGAAAGTGGCTTGATTAAAAGACTCCTTTTAAAT GGAAGCCACCAGTCAGCAGAATGGAAGCTTAGAGGAACTTGCCTGTGAGCGCTGGTCTTTGTGTTTGGTTTTGTGATGTAACGATCTTTGCTGGGGTTTT TTGCTTTGTTTTGAGGGAAATGTCTTGGAGTAAATTTTAAGTTCCTGGAGTTAATTTGTTTTACAGGAATTTTGTTTTTTAAAAAAATAGGATCATTCTG AACTTTGGAATGACCCCCTTATATATTTTCTGAAAATGAAAACAGTTACATGAAAAAAATTTCCAATGAAGATGTCAGCATTTTATGAAAAACCAGAAGT TATTAGATGAAAGCAGCGAGTGAATCTTTAAAACAGACTTGATCACGCACACACAATAAGTCTTTCTCTCCGAAACCGGAAGTAAATCTATATCTGTTAG AAATAATGTAGCCAAAAGAATGTAAATTTGAGGATTTTTTTGCCAATAGTTTATAGAAAATATATGAACCAAAGTGATTTGAGTTTGTAAAAATGTAAAA TAGTATGAACAAAATTTGCACTCTACCAGATTTGAACATCTAGTGAGGTTCACATTCATACTAAGTTTTCAACATTGTGTTCTTTTTGCATTCATTTTTT ACTTTTATTAAAGGTTCAAAACCAA

refseq_INSIG1.F1 (forward primer)
refseq_INSIG1.R1 (reverse primer)
Multiple exon skipping

Frame 0

GPRGPPYKPARPPGAAAVPGRDSSFPPPRLRSE SRRAGASRPGSASWTRVTDAQIARPLLELLLCAQ REAPRPPASQRRGAGGQGWGDDQRFRVRALPAG GPRCPGR*PRAQGPQCCDERPRARQPLPQHLAS SPVAEEPRALLGWGGPSPGAQPAADPEECHSLPR GGDRHHLFLRLVGPSLLRDSSCCCWPTVPLYRQ SPRRTPQI*ERMGQCHALHSSFCWH*PRQC*IG FCQ*CPAVLDFSSPIFGPLVDI*SFQKWPWAGDH HSFSSYADHAVSRV*WCLSVYIPRFPLYSFLAP LYIFLRRRHGGEHRTTVSYADTLL*GVEFEDHL AVSQNQK*LQGVPEKPHSD*VFKTTDSESKEDFG RKSDCGL*QRLSFFLSNLFRSG*LYK*LLEKTL HLV*NREVENDDLP*SLPLTARTGACLCPGAFC PGYVGSGRWQLPKYSISFM*LKQVAIFQSLELRL NYQPAVLCQCPKEVG*WCLTNMKYGVIGIIFIQ KIFKNRAVFKKKKQNKKSSSDYREVTL*VGSRR GHASRSRSSVLQWRVYMVTRVCITSGSTACHSSR GSLCRQSY*AKGNEKSWFPHCDILEFSPQFMKF ISKSIVI*E*IPVCHVFQS**AKIVCLLLQNRD DCFLPQPYGICNL*LPCKKESAYGTALNVWCF*S MILVSTMYSFNGIDHTRPNLQVLGLKLQVQCI* KPI*ALYLLNIYFF*RVRCSREGSPFISVLPGG NSAMISFSCEVPFVLHPPLNPQPSKYSSFVGLVI FTYKFTFLYFAIYMCLVCFKFCESGLIKRLLLN GSHQSAEWKLRGTCL*ALVFVFGFVM*RSLLGF FALF*GKCLGVNFKFLELICFTGILFFKKIGSF* TLE*PPYIFSENENSYMKKISNEDVSIL*KTRS Y*MKAASESLKQT*SRTHNKSFSPKPEVNLYLL EIM*PKECKFEDFFANSL*KIYEPK*FEFVKM*N SMNKICTLPDLNI**GSHSY*VFNIVFFLHSFF TFIKGSKP

Frame 1

GPAARHINPRARQALRPSRAVTPPFPRPASVRR AGGRAPLGQEAPLGRV*PMPRLHDHFWSCSCAH SARRRGPPRASAAGLAAKVGEMINVSVSGPSLLA AHGAPDADPAPRGRSAAMSGPEPGSPYPNTWHH RLLQRSLVLFSVGVVLALVLNLLQIQRNVTLFP EEVIATIFSSAWWVPPCCGTAAAVVGLLYPCIDS HLGEPHKFKREWASVMRCIAVFVGINHASAKLD FANNVQLSLTLAALSLGLWWTFDRSRSGLGLGI TIAFLATLITQFLVYNGVYQYTSPDFLYIRSWLP CIFFSGGVTVGNIGRQLAMLIPFCEELNLKTTW LFHKTRSNYRVFLKSPIVIESSKPPILRARKIL EENLTVDYDKDYLFS*VIYLDRADCTNDSWKKLF T*SRIGRWRMMTYPEVFP*LPALAPVCALEHSA QATWVQAGGSFPSIRFHSCD*NKLPYFKALN*D SITNPQFCVSAQRR*VDGA*QT*SMV**E*YLSK RFLKIGLCLKKKNKTRKAAVIIERSHSKWGRGV ATLHGHARPSCSGVFTWSHVCVSPVGQLLVIPP VAVCVDNLTEQKAMKSLGSHTAIYWNFHLSL*SL FRNP*SSKNEYLSAMYFNLSEPKLFVCYYRIEM TVFCHSPMEFAICDCLVKRRVHMALH*TCGVSS Q*YW*AQCIHLMA*TIPDLICKYWVLNFKCNVYE NQSEPCIS*IFIFFNV*DVREKVLHSFQCCLEE TRQ*FLSVVKFLSCYTLH*TLNLRNTPVLWVWS FLLINLPFCILQFTCVWFVLNSVKVA*LKDSF*M EATSQQNGSLEELACERWSLCLVL*CNDLCWGF LLCFEGNVLE*ILSSWS*FVLQEFCFLKK*DHS ELWNDPLIYFLKMKTVT*KKFPMKMSAFYEKPEV IR*KQRVNL*NRLDHAHTISLSLRNRK*IYIC* K*CSQKNVNLRIFLPIVYRKYMNQSDLSL*KCK IV*TKFALYQI*TSSEVHIHTKFSTLCSFCIHFL LLLKVQNQ

Frame 2

APRPAI*TRAPARRCGRPGP*LLLSPAPPPFGE PAGGRLSARKRLLDACDRCPDCTTTSGAAPVRT ARGAEAPREPAPRGWRPRLGR*STFPCPGPPCW RPTVPRTLTPRPGAAVLR*AAPSPAAPTPTPGII ACCRGASCSSRLGWS*PWCSTCCRSRGMSLSSP RR*SPPSFPPPGGSLPAAGQQLLLLAYCTPVST VTSENPTNLRENGPVSCAA*QFLLALTTPVLNWI LPIMSSCP*L*QPYLWAFGGHLIVPEVALGWGS P*LF*LR*SRSFSCIMVSISIHPQISSIFVLGS LVYFSQEASRWGT*DDS*LC*YPSVRS*I*RPLG CFTKPEVITGCS*KAP**LSLQNHRF*EQGRFW KKI*LWIMTKIIFFLK*SI*IGLTVQMTPGKNS SPSLE*GGGE**LTLKSSLDCPHWRLSVPWSILP RLRGFRQVAASQVFDFIHVIKTSCHISKP*TKT QLPTRSFVSVPKGGRLMVLNKHEVWCNRNNIYP KDF*K*GCV*KKKTKQEKQQ*L*RGHTLSGVAAW PRFTVTLVRPAVACLHGHTCVYHQWVNCLSFLP WQFV*TILLSKRQ*KVLVPTLRYIGIFTSVYEV YFEIHSHLRMNTCLPCISILVSQNCLFVTTE*R* LFFATALWNLQSVIAL*KGECIWHCIKRVVFLV NDIGEHNVFI*WHRPYQT*FASIGS*TSSAMYM KTNLSLVSLKYLFFLTCEMFERRFSIHFSAAWRK LGNDFFQL*SSFRVTPSTEPSTFEILQFCGFGH FYL*IYLFVFCNLHVFGLF*IL*KWLD*KTPFK WKPPVSRMEA*RNLPVSAGLCVWFCDVTIFAGVF CFVLREMSWSKF*VPGVNLFYRNFVF*KNRIIL NFGMTPLYIF*K*KQLHEKNFQ*RCQHFMKNQK LLDESSE*IFKTDLITHTQ*VFLSETGSKSISVR NNVAKRM*I*GFFCQ*FIENI*TKVI*VCKNVK *YEQNLHSTRFEHLVRFTFILSFQHCVLFAFIF YFY*RFKT

Sequence NM_005542

GGGCCCCGCGGCCCGCCATATAAACCCGCGCGCCCGCCAGGCGCTGCGGCCGTCCCGGGCCGTGACTCCTCCTTTCCCCCGCCCCGCCTCCGTTCGGAGA GCCGGCGGGCGGGCGCCTCTCGGCCAGGAAGCGCCTCTTGGACGCGTGTGACCGATGCCCAGATTGCACGACCACTTCTGGAGCTGCTCCTGTGCGCACA GCGCGAGGCGCCGAGGCCCCCCGCGAGCCAGCGCCGCGGGGCTGGCGGCCAAGGTTGGGGAGATGATCAACGTTTCCGTGTCCGGGCCCTCCCTGCTGGC GGCCCACGGTGCCCCGGACGCTGACCCCGCGCCCAGGGGCCGCAGTGCTGCGATGAGCGGCCCCGAGCCCGGCAGCCCCTACCCCAACACCTGGCATCAT CGCCTGTTGCAGAGGAGCCTCGTGCTCTTCTCGGTTGGGGTGGTCCTAGCCCTGGTGCTCAACCTGCTGCAGATCCAGAGGAATGTCACTCTCTTCCCCG AGGAGGTGATCGCCACCATCTTTTCCTCCGCCTGGTGGGTCCCTCCCTGCTGCGGGACAGCAGCTGCTGTTGTTGGCCTACTGTACCCCTGTATCGACAG TCACCTCGGAGAACCCCACAAATTTAAGAGAGAATGGGCCAGTGTCATGCGCTGCATAGCAGTTTTTGTTGGCATTAACCACGCCAGTGCTAAATTGGAT TTTGCCAATAATGTCCAGCTGTCCTTGACTTTAGCAGCCCTATCTTTGGGCCTTTGGTGGACATTTGATCGTTCCAGAAGTGGCCTTGGGCTGGGGATCA CCATAGCTTTTCTAGCTACGCTGATCACGCAGTTTCTCGTGTATAATGGTGTCTATCAGTATACATCCCCAGATTTCCTCTATATTCGTTCTTGGCTCCC TTGTATATTTTTCTCAGGAGGCGTCACGGTGGGGAACATAGGACGACAGTTAGCTATGGGTGTTCCTGAAAAGCCCCATAGTGATTGAGTCTTCAAAACC ACCGATTCTGAGAGCAAGGAAGATTTTGGAAGAAAATCTGACTGTGGATTATGACAAAGATTATCTTTTTTCTTAAGTAATCTATTTAGATCGGGCTGAC TGTACAAATGACTCCTGGAAAAAACTCTTCACCTAGTCTAGAATAGGGAGGTGGAGAATGATGACTTACCCTGAAGTCTTCCCTTGACTGCCCGCACTGG CGCCTGTCTGTGCCCTGGAGCATTCTGCCCAGGCTACGTGGGTTCAGGCAGGTGGCAGCTTCCCAAGTATTCGATTTCATTCATGTGATTAAAACAAGTT GCCATATTTCAAAGCCTTGAACTAAGACTCAATTACCAACCCGCAGTTTTGTGTCAGTGCCCAAAGGAGGTAGGTTGATGGTGCTTAACAAACATGAAGT ATGGTGTAATAGGAATAATATTTATCCAAAAGATTTTTAAAAATAGGGCTGTGTTTAAAAAAAAAAAACAAAACAAGAAAAGCAGCAGTGATTATAGAGA GGTCACACTCTAAGTGGGGTCGCGGCGTGGCCACGCTTCACGGTCACGCTCGTCCGTCCTGCAGTGGCGTGTTTACATGGTCACACGTGTGTGTATCACC AGTGGGTCAACTGCTTGTCATTCCTCCCGTGGCAGTTTGTGTAGACAATCTTACTGAGCAAAAGGCAATGAAAAGTCTTGGTTCCCACACTGCGATATAT TGGAATTTTCACCTCAGTTTATGAAGTTTATTTCGAAATCCATAGTCATCTAAGAATGAATACCTGTCTGCCATGTATTTCAATCTTAGTGAGCCAAAAT TGTTTGTTTGTTACTACAGAATAGAGATGACTGTTTTTTGCCACAGCCCTATGGAATTTGCAATCTGTGATTGCCTTGTAAAAAGGAGAGTGCATATGGC ACTGCATTAAACGTGTGGTGTTTCTAGTCAATGATATTGGTGAGCACAATGTATTCATTTAATGGCATAGACCATACCAGACCTAATTTGCAAGTATTGG GTCTTAAACTTCAAGTGCAATGTATATGAAAACCAATCTGAGCCTTGTATCTCTTAAATATTTATTTTTTTTAACGTGTGAGATGTTCGAGAGAAGGTTC TCCATTCATTTCAGTGCTGCCTGGAGGAAACTCGGCAATGATTTCTTTCAGTTGTGAAGTTCCTTTCGTGTTACACCCTCCACTGAACCCTCAACCTTCG AAATACTCCAGTTTTGTGGGTTTGGTCATTTTTACTTATAAATTTACCTTTTTGTATTTTGCAATTTACATGTGTTTGGTTTGTTTTAAATTCTGTGAAA GTGGCTTGATTAAAAGACTCCTTTTAAATGGAAGCCACCAGTCAGCAGAATGGAAGCTTAGAGGAACTTGCCTGTGAGCGCTGGTCTTTGTGTTTGGTTT TGTGATGTAACGATCTTTGCTGGGGTTTTTTGCTTTGTTTTGAGGGAAATGTCTTGGAGTAAATTTTAAGTTCCTGGAGTTAATTTGTTTTACAGGAATT TTGTTTTTTAAAAAAATAGGATCATTCTGAACTTTGGAATGACCCCCTTATATATTTTCTGAAAATGAAAACAGTTACATGAAAAAAATTTCCAATGAAG ATGTCAGCATTTTATGAAAAACCAGAAGTTATTAGATGAAAGCAGCGAGTGAATCTTTAAAACAGACTTGATCACGCACACACAATAAGTCTTTCTCTCC GAAACCGGAAGTAAATCTATATCTGTTAGAAATAATGTAGCCAAAAGAATGTAAATTTGAGGATTTTTTTGCCAATAGTTTATAGAAAATATATGAACCA AAGTGATTTGAGTTTGTAAAAATGTAAAATAGTATGAACAAAATTTGCACTCTACCAGATTTGAACATCTAGTGAGGTTCACATTCATACTAAGTTTTCA ACATTGTGTTCTTTTTGCATTCATTTTTTACTTTTATTAAAGGTTCAAAACCAA

refseq_INSIG1.F1 (forward primer)
refseq_INSIG1.R1 (reverse primer)
Multiple exon skipping

Frame 0

GPRGPPYKPARPPGAAAVPGRDSSFPPPRLRSE SRRAGASRPGSASWTRVTDAQIARPLLELLLCAQ REAPRPPASQRRGAGGQGWGDDQRFRVRALPAG GPRCPGR*PRAQGPQCCDERPRARQPLPQHLAS SPVAEEPRALLGWGGPSPGAQPAADPEECHSLPR GGDRHHLFLRLVGPSLLRDSSCCCWPTVPLYRQ SPRRTPQI*ERMGQCHALHSSFCWH*PRQC*IG FCQ*CPAVLDFSSPIFGPLVDI*SFQKWPWAGDH HSFSSYADHAVSRV*WCLSVYIPRFPLYSFLAP LYIFLRRRHGGEHRTTVSYGCS*KAP**LSLQN HRF*EQGRFWKKI*LWIMTKIIFFLK*SI*IGLT VQMTPGKNSSPSLE*GGGE**LTLKSSLDCPHW RLSVPWSILPRLRGFRQVAASQVFDFIHVIKTS CHISKP*TKTQLPTRSFVSVPKGGRLMVLNKHEV WCNRNNIYPKDF*K*GCV*KKKTKQEKQQ*L*R GHTLSGVAAWPRFTVTLVRPAVACLHGHTCVYH QWVNCLSFLPWQFV*TILLSKRQ*KVLVPTLRYI GIFTSVYEVYFEIHSHLRMNTCLPCISILVSQN CLFVTTE*R*LFFATALWNLQSVIAL*KGECIW HCIKRVVFLVNDIGEHNVFI*WHRPYQT*FASIG S*TSSAMYMKTNLSLVSLKYLFFLTCEMFERRF SIHFSAAWRKLGNDFFQL*SSFRVTPSTEPSTF EILQFCGFGHFYL*IYLFVFCNLHVFGLF*IL*K WLD*KTPFKWKPPVSRMEA*RNLPVSAGLCVWF CDVTIFAGVFCFVLREMSWSKF*VPGVNLFYRN FVF*KNRIILNFGMTPLYIF*K*KQLHEKNFQ*R CQHFMKNQKLLDESSE*IFKTDLITHTQ*VFLS ETGSKSISVRNNVAKRM*I*GFFCQ*FIENI*T KVI*VCKNVK*YEQNLHSTRFEHLVRFTFILSFQ HCVLFAFIFYFY*RFKT

Frame 1

GPAARHINPRARQALRPSRAVTPPFPRPASVRR AGGRAPLGQEAPLGRV*PMPRLHDHFWSCSCAH SARRRGPPRASAAGLAAKVGEMINVSVSGPSLLA AHGAPDADPAPRGRSAAMSGPEPGSPYPNTWHH RLLQRSLVLFSVGVVLALVLNLLQIQRNVTLFP EEVIATIFSSAWWVPPCCGTAAAVVGLLYPCIDS HLGEPHKFKREWASVMRCIAVFVGINHASAKLD FANNVQLSLTLAALSLGLWWTFDRSRSGLGLGI TIAFLATLITQFLVYNGVYQYTSPDFLYIRSWLP CIFFSGGVTVGNIGRQLAMGVPEKPHSD*VFKT TDSESKEDFGRKSDCGL*QRLSFFLSNLFRSG* LYK*LLEKTLHLV*NREVENDDLP*SLPLTARTG ACLCPGAFCPGYVGSGRWQLPKYSISFM*LKQV AIFQSLELRLNYQPAVLCQCPKEVG*WCLTNMK YGVIGIIFIQKIFKNRAVFKKKKQNKKSSSDYRE VTL*VGSRRGHASRSRSSVLQWRVYMVTRVCIT SGSTACHSSRGSLCRQSY*AKGNEKSWFPHCDI LEFSPQFMKFISKSIVI*E*IPVCHVFQS**AKI VCLLLQNRDDCFLPQPYGICNL*LPCKKESAYG TALNVWCF*SMILVSTMYSFNGIDHTRPNLQVL GLKLQVQCI*KPI*ALYLLNIYFF*RVRCSREGS PFISVLPGGNSAMISFSCEVPFVLHPPLNPQPS KYSSFVGLVIFTYKFTFLYFAIYMCLVCFKFCE SGLIKRLLLNGSHQSAEWKLRGTCL*ALVFVFGF VM*RSLLGFFALF*GKCLGVNFKFLELICFTGI LFFKKIGSF*TLE*PPYIFSENENSYMKKISNE DVSIL*KTRSY*MKAASESLKQT*SRTHNKSFSP KPEVNLYLLEIM*PKECKFEDFFANSL*KIYEP K*FEFVKM*NSMNKICTLPDLNI**GSHSY*VF NIVFFLHSFFTFIKGSKP

Frame 2

APRPAI*TRAPARRCGRPGP*LLLSPAPPPFGE PAGGRLSARKRLLDACDRCPDCTTTSGAAPVRT ARGAEAPREPAPRGWRPRLGR*STFPCPGPPCW RPTVPRTLTPRPGAAVLR*AAPSPAAPTPTPGII ACCRGASCSSRLGWS*PWCSTCCRSRGMSLSSP RR*SPPSFPPPGGSLPAAGQQLLLLAYCTPVST VTSENPTNLRENGPVSCAA*QFLLALTTPVLNWI LPIMSSCP*L*QPYLWAFGGHLIVPEVALGWGS P*LF*LR*SRSFSCIMVSISIHPQISSIFVLGS LVYFSQEASRWGT*DDS*LWVFLKSPIVIESSKP PILRARKILEENLTVDYDKDYLFS*VIYLDRAD CTNDSWKKLFT*SRIGRWRMMTYPEVFP*LPAL APVCALEHSAQATWVQAGGSFPSIRFHSCD*NKL PYFKALN*DSITNPQFCVSAQRR*VDGA*QT*S MV**E*YLSKRFLKIGLCLKKKNKTRKAAVIIE RSHSKWGRGVATLHGHARPSCSGVFTWSHVCVSP VGQLLVIPPVAVCVDNLTEQKAMKSLGSHTAIY WNFHLSL*SLFRNP*SSKNEYLSAMYFNLSEPK LFVCYYRIEMTVFCHSPMEFAICDCLVKRRVHMA LH*TCGVSSQ*YW*AQCIHLMA*TIPDLICKYW VLNFKCNVYENQSEPCIS*IFIFFNV*DVREKV LHSFQCCLEETRQ*FLSVVKFLSCYTLH*TLNLR NTPVLWVWSFLLINLPFCILQFTCVWFVLNSVK VA*LKDSF*MEATSQQNGSLEELACERWSLCLV L*CNDLCWGFLLCFEGNVLE*ILSSWS*FVLQEF CFLKK*DHSELWNDPLIYFLKMKTVT*KKFPMK MSAFYEKPEVIR*KQRVNL*NRLDHAHTISLSL RNRK*IYIC*K*CSQKNVNLRIFLPIVYRKYMNQ SDLSL*KCKIV*TKFALYQI*TSSEVHIHTKFS TLCSFCIHFLLLLKVQNQ