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Sequence NM_201517
GGCCACAGCAGCGCGAGGCGGGCACGGGGTATTGTCCGGCTCCGGCGGCGGCGGTCGGTGCTGCGAGAGCGGCGGCGGCGGCGCGGGTCGGCAGCGGGAGGGCGCGCGGCCGAGCGGAGGCGGAGTCGGCGCCGAGAACATGGCTGGAGGCAAAGCTGGAAAGGACAGTGGGAAGGCCAAGGCTAAGGCAGTATCTCGCT
CACAGAGAGCTGGGCTACAGGTGCTGGAGCTGGCAGGTAATGCTTCTAAGGATCTCAAAGTAAAGCGTATCACTCCGCGTCACTTGCAGCTTGCAATCCG
TGGTGATGAAGAGTTGGATTCTCTTATCAAGGCTACCATAGCTGGGGGTGGTGTGATCCCTCACATCCACAAATCTCTGATTGGAAAGAAGGGACAGCAG
AAAACTGCTTAGAGGGATGCTTTAACCAACCCTCTTCCTCCCCGTCATTGTACTGTAACTGGGACAGAAGAAATAATGGGGATATGTGGAATTTTTAACA
ACAGTTAAATGGAAAAGCATAGACAATTACTGTAGACATGATAAAAGAAACATTTGTATGTTCTTAGACTCGAAGTTTGATAAAAGTACCTTTTCATGTG
GTGACAGTTGTGTGTTGATTGGCTAGGTTTCTCCCGTGTGTTTTATACAAAAATGGAATTGATAAACCATTTTTTACAAAATTAATTTGTCTCAAAACTG
TTCTGTTCATGATGTATTAGAAATATTTTACTCAGACTTTAAATATTTTAAATCTCAGATTGGTTATTCAGAGTAACCTTAGAACAGAAATTGGGAATAT
ATCTTTACAATGATTGATACCATGGTATATTGACTCTTAGATGCTATTGATCTGTAGCACCATTTTTTACAAACGACTAAGGAAAAAACCTGCCAATTAA
ATCATGATATGCCATCAATTATGAGACATCCCAATTTGAGAGATGTTAGATTATAGAAAAGTATGCATTTATGACTGAAATGGTAGTGGAATTATTTGAA
TTCTACACCAAGCACTTACCATGTGCCAGGCCCTTTGCA
- refseq_H2AFV.F5 (forward primer)
- refseq_H2AFV.R5 (reverse primer)
- Single exon skipping
Frame 0
GHSSARRARGIVRLRRRRSVLRERRRRRGSAAGGRAAERRRSRRREHGWRQSWKGQWEGQG*GSISL
TESWATGAGAGR*CF*GSQSKAYHSASLAACNP
W**RVGFSYQGYHSWGWCDPSHPQISDWKEGTA
ENCLEGCFNQPSSSPSLYCNWDRRNNGDMWNF*Q
QLNGKA*TITVDMIKETFVCS*TRSLIKVPFHV
VTVVC*LARFLPCVLYKNGIDKPFFTKLICLKT
VLFMMY*KYFTQTLNILNLRLVIQSNLRTEIGNI
SLQ*LIPWYIDS*MLLICSTIFYKRLRKKPAN*
IMICHQL*DIPI*EMLDYRKVCIYD*NGSGII*
ILHQALTMCQALC
Frame 1
ATAARGGHGVLSGSGGGGRCCESGGGGAGRQREGARPSGGGVGAENMAGGKAGKDSGKAKAKAVSR
SQRAGLQVLELAGNASKDLKVKRITPRHLQLAIR
GDEELDSLIKATIAGGGVIPHIHKSLIGKKGQQ
KTA*RDALTNPLPPRHCTVTGTEEIMGICGIFN
NS*MEKHRQLL*T**KKHLYVLRLEV**KYLFMW
*QLCVDWLGFSRVFYTKMELINHFLQN*FVSKL
FCS*CIRNILLRL*IF*ISDWLFRVTLEQKLGI
YLYND*YHGILTLRCY*SVAPFFTND*GKNLPIK
S*YAINYETSQFERC*IIEKYAFMTEMVVELFE
FYTKHLPCARPFA
Frame 2
PQQREAGTGYCPAPAAAVGAARAAAAARVGSGRARGRAEAESAPRTWLEAKLERTVGRPRLRQYLA
HRELGYRCWSWQVMLLRISK*SVSLRVTCSLQS
VVMKSWILLSRLP*LGVV*SLTSTNL*LERRDSR
KLLRGML*PTLFLPVIVL*LGQKK*WGYVEFLT
TVKWKSIDNYCRHDKRNICMFLDSKFDKSTFSC
GDSCVLIG*VSPVCFIQKWN**TIFYKINLSQNC
SVHDVLEIFYSDFKYFKSQIGYSE*P*NRNWEY
IFTMIDTMVY*LLDAIDL*HHFLQTTKEKTCQL
NHDMPSIMRHPNLRDVRL*KSMHL*LKW*WNYLN
STPSTYHVPGPL
Sequence NM_012412
GGCCACAGCAGCGCGAGGCGGGCACGGGGTATTGTCCGGCTCCGGCGGCGGCGGTCGGTGCTGCGAGAGCGGCGGCGGCGGCGCGGGTCGGCAGCGGGAGGGCGCGCGGCCGAGCGGAGGCGGAGTCGGCGCCGAGAACATGGCTGGAGGCAAAGCTGGAAAGGACAGTGGGAAGGCCAAGGCTAAGGCAGTATCTCGCT
CACAGAGAGCTGGGCTACAGTTTCCTGTGGGCCGCATCCACAGACACTTGAAGACTCGCACCACAAGCCATGGAAGGGTGGGTGCCACTGCTGCCGTGTA
CAGTGCTGCGATTCTGGAGTACCTCACTGCAGAGGTGCTGGAGCTGGCAGGTAATGCTTCTAAGGATCTCAAAGTAAAGCGTATCACTCCGCGTCACTTG
CAGCTTGCAATCCGTGGTGATGAAGAGTTGGATTCTCTTATCAAGGCTACCATAGCTGGGGGTGGTGTGATCCCTCACATCCACAAATCTCTGATTGGAA
AGAAGGGACAGCAGAAAACTGCTTAGAGGGATGCTTTAACCAACCCTCTTCCTCCCCGTCATTGTACTGTAACTGGGACAGAAGAAATAATGGGGATATG
TGGAATTTTTAACAACAGTTAAATGGAAAAGCATAGACAATTACTGTAGACATGATAAAAGAAACATTTGTATGTTCTTAGACTCGAAGTTTGATAAAAG
TACCTTTTCATGTGGTGACAGTTGTGTGTTGATTGGCTAGGTTTCTCCCGTGTGTTTTATACAAAAATGGAATTGATAAACCATTTTTTACAAAATTAAT
TTGTCTCAAAACTGTTCTGTTCATGATGTATTAGAAATATTTTACTCAGACTTTAAATATTTTAAATCTCAGATTGGTTATTCAGAGTAACCTTAGAACA
GAAATTGGGAATATATCTTTACAATGATTGATACCATGGTATATTGACTCTTAGATGCTATTGATCTGTAGCACCATTTTTTACAAACGACTAAGGAAAA
AACCTGCCAATTAAATCATGATATGCCATCAATTATGAGACATCCCAATTTGAGAGATGTTAGATTATAGAAAAGTATGCATTTATGACTGAAATGGTAG
TGGAATTATTTGAATTCTACACCAAGCACTTACCATGTGCCAGGCCCTTTGCA
- refseq_H2AFV.F5 (forward primer)
- refseq_H2AFV.R5 (reverse primer)
- Single exon skipping
Frame 0
GHSSARRARGIVRLRRRRSVLRERRRRRGSAAGGRAAERRRSRRREHGWRQSWKGQWEGQG*GSISL
TESWATVSCGPHPQTLEDSHHKPWKGGCHCCRV
QCCDSGVPHCRGAGAGR*CF*GSQSKAYHSASL
AACNPW**RVGFSYQGYHSWGWCDPSHPQISDWK
EGTAENCLEGCFNQPSSSPSLYCNWDRRNNGDM
WNF*QQLNGKA*TITVDMIKETFVCS*TRSLIK
VPFHVVTVVC*LARFLPCVLYKNGIDKPFFTKLI
CLKTVLFMMY*KYFTQTLNILNLRLVIQSNLRT
EIGNISLQ*LIPWYIDS*MLLICSTIFYKRLRK
KPAN*IMICHQL*DIPI*EMLDYRKVCIYD*NGS
GII*ILHQALTMCQALC
Frame 1
ATAARGGHGVLSGSGGGGRCCESGGGGAGRQREGARPSGGGVGAENMAGGKAGKDSGKAKAKAVSR
SQRAGLQFPVGRIHRHLKTRTTSHGRVGATAAVY
SAAILEYLTAEVLELAGNASKDLKVKRITPRHL
QLAIRGDEELDSLIKATIAGGGVIPHIHKSLIG
KKGQQKTA*RDALTNPLPPRHCTVTGTEEIMGIC
GIFNNS*MEKHRQLL*T**KKHLYVLRLEV**K
YLFMW*QLCVDWLGFSRVFYTKMELINHFLQN*
FVSKLFCS*CIRNILLRL*IF*ISDWLFRVTLEQ
KLGIYLYND*YHGILTLRCY*SVAPFFTND*GK
NLPIKS*YAINYETSQFERC*IIEKYAFMTEMV
VELFEFYTKHLPCARPFA
Frame 2
PQQREAGTGYCPAPAAAVGAARAAAAARVGSGRARGRAEAESAPRTWLEAKLERTVGRPRLRQYLA
HRELGYSFLWAASTDT*RLAPQAMEGWVPLLPC
TVLRFWSTSLQRCWSWQVMLLRISK*SVSLRVTC
SLQSVVMKSWILLSRLP*LGVV*SLTSTNL*LE
RRDSRKLLRGML*PTLFLPVIVL*LGQKK*WGY
VEFLTTVKWKSIDNYCRHDKRNICMFLDSKFDKS
TFSCGDSCVLIG*VSPVCFIQKWN**TIFYKIN
LSQNCSVHDVLEIFYSDFKYFKSQIGYSE*P*N
RNWEYIFTMIDTMVY*LLDAIDL*HHFLQTTKEK
TCQLNHDMPSIMRHPNLRDVRL*KSMHL*LKW*
WNYLNSTPSTYHVPGPL
Sequence NM_138635
GGCCACAGCAGCGCGAGGCGGGCACGGGGTATTGTCCGGCTCCGGCGGCGGCGGTCGGTGCTGCGAGAGCGGCGGCGGCGGCGCGGGTCGGCAGCGGGAGGGCGCGCGGCCGAGCGGAGGCGGAGTCGGCGCCGAGAACATGGCTGGAGGCAAAGCTGGAAAGGACAGTGGGAAGGCCAAGGCTAAGGCAGTATCTCGCT
CACAGAGAGCTGGGCTACAGTTTCCTGTGGGCCGCATCCACAGACACTTGAAGACTCGCACCACAAGCCATGGAAGGGTGGGTGCCACTGCTGCCGTGTA
CAGTGCTGCGATTCTGGAGTACCTCACTGCAGAGGTGCTGGAGCTGGCAGGTAATGCTTCTAAGGATCTCAAAGTAAAGCGTATCACTCCGCGTCACTTG
CAGCTTGCAATCCGTGGTGATGAAGAGTTGGATTCTCTTATCAAGGCTACCATAGCTGGGGGTGAGAAGAGAAGGTGTTCTTAGATCAGAAGATGCCATT
GTATTAGGTGACGGCAAGGTTTTGCTGATTTAGTGGAACAAGATTCTACTGGGATGTGTGCTGAGCTGAACCAAGGAACTGCCCGAATGTGCTGAACAAG
GACATTTGCTTCTCAGATACGTGAATTCAATTTTAAGCAAGATTCTTGATCTGCTTCAGAGCAGCTTTGATTTAAAGTAATTTCAGAGCGCTTTTCCTTG
CATGAGTAATTTTCTGAATGTATAAAATATTCTATTTATGTTTGACCTTTTGGATAAACTGTGTTGCTGCAGTGTTGGTCCACACTACCATCCTAAGTGA
AGACTTAGTAAGTCTGAGGATCTCC
- refseq_H2AFV.F5 (forward primer)
- refseq_H2AFV.R5 (reverse primer)
- Single exon skipping
Frame 0
GHSSARRARGIVRLRRRRSVLRERRRRRGSAAGGRAAERRRSRRREHGWRQSWKGQWEGQG*GSISL
TESWATVSCGPHPQTLEDSHHKPWKGGCHCCRV
QCCDSGVPHCRGAGAGR*CF*GSQSKAYHSASL
AACNPW**RVGFSYQGYHSWG*EEKVFLDQKMPL
Y*VTARFC*FSGTRFYWDVC*AEPRNCPNVLNK
DICFSDT*IQF*ARFLICFRAALI*SNFRALFL
A*VIF*MYKIFYLCLTFWINCVAAVLVHTTILSE
DLVSLRIS
Frame 1
ATAARGGHGVLSGSGGGGRCCESGGGGAGRQREGARPSGGGVGAENMAGGKAGKDSGKAKAKAVSR
SQRAGLQFPVGRIHRHLKTRTTSHGRVGATAAVY
SAAILEYLTAEVLELAGNASKDLKVKRITPRHL
QLAIRGDEELDSLIKATIAGGEKRRCS*IRRCH
CIR*RQGFADLVEQDSTGMCAELNQGTARMC*TR
TFASQIREFNFKQDS*SASEQL*FKVISERFSL
HE*FSECIKYSIYV*PFG*TVLLQCWSTLPS*V
KT**V*GS
Frame 2
PQQREAGTGYCPAPAAAVGAARAAAAARVGSGRARGRAEAESAPRTWLEAKLERTVGRPRLRQYLA
HRELGYSFLWAASTDT*RLAPQAMEGWVPLLPC
TVLRFWSTSLQRCWSWQVMLLRISK*SVSLRVTC
SLQSVVMKSWILLSRLP*LGVRREGVLRSEDAI
VLGDGKVLLI*WNKILLGCVLS*TKELPECAEQ
GHLLLRYVNSILSKILDLLQSSFDLK*FQSAFPC
MSNFLNV*NILFMFDLLDKLCCCSVGPHYHPK*
RLSKSEDL
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