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Sequences

Sequence NM_175728

refseq_IL5RA.F2 (forward primer)
refseq_IL5RA.R2 (reverse primer)
Single exon skipping

Frame 0

Frame 1

Frame 2

Sequence NM_175726

AGTACCCACGGAAAACTTGTTTACCTTGTCACCATGAGTAAAAGTTAATTCCCACTCCTGAAGAGAGCAAACCAACTCTGAAAGAGAGTGAAAATGCAGA CAAGACAGTTATCAGATAATGGCTATCTGGACGAGAGATTCTTTCGTTTGACAGCAGTTTGGTTGTTGGGAGTTCCAGTTCAGCTCCTGCACAGTTGCTC TGTACAAATCCTCCTCCATATTTGCTTAGAGAAAACGTGTTGCCATCCCATCATGAAGGAAGCTGCCTGAGAGTTTTTAACCATTACAGCCGTGATGATG AAAGAGTGAAGAACCGCCTCTAAGTTAAAAAGTGCACCCAGAGATAAGGTTCGTTCTCAATGCCCTGCCGCTGCTTCTCATCGCATGGCCACCGCATTTC TCAGGCCAGGCACATTGAGCATTGGTCCTGTGCCTGACGCTATGCTAGATGCTGGGGTTGCAGCCACGAGCATAGACACGACAGACACGGTCCTCGCCAT CTTCTGTTGAGTACTGGTCGGAACAAGAGGATCGTCTGTAGACAGGATATGATCATCGTGGCGCATGTATTACTCATCCTTTTGGGGGCCACTGAGATAC TGCAAGCTGACTTACTTCCTGATGAAAAGATTTCACTTCTCCCACCTGTCAATTTCACCATTAAAGTTACTGGTTTGGCTCAAGTTCTTTTACAATGGAA ACCAAATCCTGATCAAGAGCAAAGGAATGTTAATCTAGAATATCAAGTGAAAATAAACGCTCCAAAAGAAGATGACTATGAAACCAGAATCACTGAAAGC AAATGTGTAACCATCCTCCACAAAGGCTTTTCAGCAAGTGTGCGGACCATCCTGCAGAACGACCACTCACTACTGGCCAGCAGCTGGGCTTCTGCTGAAC TTCATGCCCCACCAGGGTCTCCTGGAACCTCAATTGTGAATTTAACTTGCACCACAAACACTACAGAAGACAATTATTCACGTTTAAGGTCATACCAAGT TTCCCTTCACTGCACCTGGCTTGTTGGCACAGATGCCCCTGAGGACACGCAGTATTTTCTCTACTATAGGTATGGCTCTTGGACTGAAGAATGCCAAGAA TACAGCAAAGACACACTGGGGAGAAATATCGCATGCTGGTTTCCCAGGACTTTTATCCTCAGCAAAGGGCGTGACTGGCTTGCGGTGCTTGTTAACGGCT CCAGCAAGCACTCTGCTATCAGGCCCTTTGATCAGCTGTTTGCCCTTCACGCCATTGATCAAATAAATCCTCCACTGAATGTCACAGCAGAGATTGAAGG AACTCGTCTCTCTATCCAATGGGAGAAACCAGTGTCTGCTTTTCCAATCCATTGCTTTGATTATGAAGTAAAAATACACAATACAAGGAATGGATATTTG CAGATAGAAAAATTGATGACCAATGCATTCATCTCAATAATTGATGATCTTTCTAAGTACGATGTTCAAGTGAGAGCAGCAGTGAGCTCCATGTGCAGAG AGGCAGGGCTCTGGAGTGAGTGGAGCCAACCTATTTATGTGGGAAATGATGAACACAAGCCCTTGAGAGAGTGGTTTGTCATTGTGATTATGGCAACCAT CTGCTTCATCTTGTTAATTCTCTCGCTTATCTGTAAAATATGTCATTTATGGATCAAGTTGTTTCCACCAATTCCAGCACCAAAAAGTAATATCAAAGAT CTCTTTGTAACCACTAACTATGAGAAAGCTGGGTCCAGTGAGACGGAAATTGAAGTCATCTGTTATATAGAGAAGCCTGGAGTTGAGACCCTGGAGGATT CTGTGTTTTGACTGTCACTTTGGCATCCTCTGATGAACTCACACATGCCTCAGTGCCTCAGTGAAAAGAACAGGGATGCTGGCTCTTGGCTAAGAGGTGT TCAGAATTTAGGCAACACTCAATTTACCTGCGAAGCAATACACCCAGACACACCAGTCTTGTATCTCTTAAAAGTATGGATGCTTCATCCAAATCGCCTC ACCTACAGCAGGGAAGTTGACTCATCCAAGCATTTTGCCATGTTTTTTCTCCCCATGCCGTACAGGGTAGCACCTCCTCACCTGCCAATCTTTGCAATTT GCTTGACTCACCTCAGACTTTTCATTCACAACAGACAGCTTTTAAGGCTAACGTCCAGCTGTATTTACTTCTGGCTGTGCCCGTTTGGCTGTTTAAGCTG CCAATTGTAGCACTCAGCTACCATCTGAGGAAGAAAGCATTTTGCATCAGCCTGGAGTGAACCATGAACTTGGATTCAAGACTGTCTTTTCTATAGCAA

refseq_IL5RA.F2 (forward primer)
refseq_IL5RA.R2 (reverse primer)
Single exon skipping

Frame 0

STHGKLVYLVTMSKS*FPLLKRANQL*KRVKMQ TRQLSDNGYLDERFFRLTAVWLLGVPVQLLHSCS VQILLHICLEKTCCHPIMKEAA*EFLTITAVMM KE*RTASKLKSAPRDKVRSQCPAAASHRMATAF LRPGTLSIGPVPDAMLDAGVAATSIDTTDTVLAI FC*VLVGTRGSSVDRI*SSWRMYYSSFWGPLRY CKLTYFLMKRFHFSHLSISPLKLLVWLKFFYNG NQILIKSKGMLI*NIK*K*TLQKKMTMKPESLKA NV*PSSTKAFQQVCGPSCRTTTHYWPAAGLLLN FMPHQGLLEPQL*I*LAPQTLQKTIIHV*GHTK FPFTAPGLLAQMPLRTRSIFSTIGMALGLKNAKN TAKTHWGEISHAGFPGLLSSAKGVTGLRCLLTA PASTLLSGPLISCLPFTPLIK*ILH*MSQQRLK ELVSLSNGRNQCLLFQSIALIMK*KYTIQGMDIC R*KN**PMHSSQ*LMIFLSTMFK*EQQ*APCAE RQGSGVSGANLFMWEMMNTSP*ESGLSL*LWQP SASSC*FSRLSVKYVIYGSSCFHQFQHQKVISKI SL*PLTMRKLGPVRRKLKSSVI*RSLELRPWRI LCFDCHFGIL**THTCLSASVKRTGMLALG*EV FRI*ATLNLPAKQYTQTHQSCIS*KYGCFIQIAS PTAGKLTHPSILPCFFSPCRTG*HLLTCQSLQF A*LTSDFSFTTDSF*G*RPAVFTSGCARLAV*A ANCSTQLPSEEESILHQPGVNHELGFKTVFSIA

Frame 1

VPTENLFTLSP*VKVNSHS*REQTNSERE*KCR QDSYQIMAIWTRDSFV*QQFGCWEFQFSSCTVA LYKSSSIFA*RKRVAIPS*RKLPESF*PLQP*** KSEEPPLS*KVHPEIRFVLNALPLLLIAWPPHF SGQAH*ALVLCLTLC*MLGLQPRA*TRQTRSSP SSVEYWSEQEDRL*TGYDHRGACITHPFGGH*DT AS*LTS**KDFTSPTCQFHH*SYWFGSSSFTME TKS*SRAKEC*SRISSENKRSKRR*L*NQNH*K QMCNHPPQRLFSKCADHPAERPLTTGQQLGFC*T SCPTRVSWNLNCEFNLHHKHYRRQLFTFKVIPS FPSLHLACWHRCP*GHAVFSLL*VWLLD*RMPR IQQRHTGEKYRMLVSQDFYPQQRA*LACGAC*RL QQALCYQAL*SAVCPSRH*SNKSSTECHSRD*R NSSLYPMGETSVCFSNPLL*L*SKNTQYKEWIF ADRKIDDQCIHLNN**SF*VRCSSESSSELHVQR GRALE*VEPTYLCGK**TQALERVVCHCDYGNH LLHLVNSLAYL*NMSFMDQVVSTNSSTKK*YQR SLCNH*L*ESWVQ*DGN*SHLLYREAWS*DPGGF CVLTVTLASSDELTHASVPQ*KEQGCWLLAKRC SEFRQHSIYLRSNTPRHTSLVSLKSMDASSKSP HLQQGS*LIQAFCHVFSPHAVQGSTSSPANLCNL LDSPQTFHSQQTAFKANVQLYLLLAVPVWLFKL PIVALSYHLRKKAFCISLE*TMNLDSRLSFL*Q

Frame 2

YPRKTCLPCHHE*KLIPTPEESKPTLKESENAD KTVIR*WLSGREILSFDSSLVVGSSSSAPAQLL CTNPPPYLLRENVLPSHHEGSCLRVFNHYSRDD ERVKNRL*VKKCTQR*GSFSMPCRCFSSHGHRIS QARHIEHWSCA*RYARCWGCSHEHRHDRHGPRH LLLSTGRNKRIVCRQDMIIVAHVLLILLGATEI LQADLLPDEKISLLPPVNFTIKVTGLAQVLLQWK PNPDQEQRNVNLEYQVKINAPKEDDYETRITES KCVTILHKGFSASVRTILQNDHSLLASSWASAE LHAPPGSPGTSIVNLTCTTNTTEDNYSRLRSYQV SLHCTWLVGTDAPEDTQYFLYYRYGSWTEECQE YSKDTLGRNIACWFPRTFILSKGRDWLAVLVNG SSKHSAIRPFDQLFALHAIDQINPPLNVTAEIEG TRLSIQWEKPVSAFPIHCFDYEVKIHNTRNGYL QIEKLMTNAFISIIDDLSKYDVQVRAAVSSMCR EAGLWSEWSQPIYVGNDEHKPLREWFVIVIMATI CFILLILSLICKICHLWIKLFPPIPAPKSNIKD LFVTTNYEKAGSSETEIEVICYIEKPGVETLED SVF*LSLWHPLMNSHMPQCLSEKNRDAGSWLRGV QNLGNTQFTCEAIHPDTPVLYLLKVWMLHPNRL TYSREVDSSKHFAMFFLPMPYRVAPPHLPIFAI CLTHLRLFIHNRQLLRLTSSCIYFWLCPFGCLSC QL*HSATI*GRKHFASAWSEP*TWIQDCLFYS

Sequence NM_175727

AGTACCCACGGAAAACTTGTTTACCTTGTCACCATGAGTAAAAGTTAATTCCCACTCCTGAAGAGAGCAAACCAACTCTGAAAGAGAGTGAAAATGCAGA CAAGACAGTTATCAGATAATGGCTATCTGGACGAGAGATTCTTTCGTTTGACAGCAGTTTGGTTGTTGGGAGTTCCAGTTCAGCTCCTGCACAGTTGCTC TGTACAAATCCTCCTCCATATTTGCTTAGAGAAAACGTGTTGCCATCCCATCATGAAGGAAGCTGCCTGAGAGTTTTTAACCATTACAGCCGTGATGATG AAAGAGTGAAGAACCGCCTCTAAGTTAAAAAGTGCACCCAGAGATAAGGTTCGTTCTCAATGCCCTGCCGCTGCTTCTCATCGCATGGCCACCGCATTTC TCAGGCCAGGCACATTGAGCATTGGTCCTGTGCCTGACGCTATGCTAGATGCTGGGGTTGCAGCCACGAGCATAGACACGACAGACACGGTCCTCGCCAT CTTCTGTTGAGTACTGGTCGGAACAAGAGGATCGTCTGTAGACAGGATATGATCATCGTGGCGCATGTATTACTCATCCTTTTGGGGGCCACTGAGATAC TGCAAGCTGACTTACTTCCTGATGAAAAGATTTCACTTCTCCCACCTGTCAATTTCACCATTAAAGTTACTGGTTTGGCTCAAGTTCTTTTACAATGGAA ACCAAATCCTGATCAAGAGCAAAGGAATGTTAATCTAGAATATCAAGTGAAAATAAACGCTCCAAAAGAAGATGACTATGAAACCAGAATCACTGAAAGC AAATGTGTAACCATCCTCCACAAAGGCTTTTCAGCAAGTGTGCGGACCATCCTGCAGAACGACCACTCACTACTGGCCAGCAGCTGGGCTTCTGCTGAAC TTCATGCCCCACCAGGGTCTCCTGGAACCTCAATTGTGAATTTAACTTGCACCACAAACACTACAGAAGACAATTATTCACGTTTAAGGTCATACCAAGT TTCCCTTCACTGCACCTGGCTTGTTGGCACAGATGCCCCTGAGGACACGCAGTATTTTCTCTACTATAGGTATGGCTCTTGGACTGAAGAATGCCAAGAA TACAGCAAAGACACACTGGGGAGAAATATCGCATGCTGGTTTCCCAGGACTTTTATCCTCAGCAAAGGGCGTGACTGGCTTGCGGTGCTTGTTAACGGCT CCAGCAAGCACTCTGCTATCAGGCCCTTTGATCAGCTGTTTGCCCTTCACGCCATTGATCAAATAAATCCTCCACTGAATGTCACAGCAGAGATTGAAGG AACTCGTCTCTCTATCCAATGGGAGAAACCAGTGTCTGCTTTTCCAATCCATTGCTTTGATTATGAAGTAAAAATACACAATACAAGGAATGGATATTTG CAGATAGAAAAATTGATGACCAATGCATTCATCTCAATAATTGATGATCTTTCTAAGTACGATGTTCAAGTGAGAGCAGCAGTGAGCTCCATGTGCAGAG AGGCAGGGCTCTGGAGTGAGTGGAGCCAACCTATTTATGTGGGTAAGTAGCTTATGTTTATTTTACATTGGCAGCCTTCCTTGTGATCAAAAAAGGTAAT CCCAGAAACGTACCCGTTCACTCGTGGGTCTTAAAATGGTTTCATATCTCTATTGTGACTAATTTTCTCTCGGTCTACTGCCTTTTCAATCAGGAATAGA TTTGCCATGAAGCCAGTGAAGTTTTTAAGTGTCTAGGCTTCTCATTAGCGCCAACTCTCCTAGACCTGGTGCCTGTTTTTTTTCCAAGTTTTGTTTCTAC TTCTATCCATTTTTTAAATTAAACTTTTTATTTTGAAATAATTATCACACTCACAAGCTGTGGGAAGAAATAATAGAGATCCTGTGTCTCTTTCATCCAG TTTTCCTCAAGGGTAACATCTTACAAAACTATAGTACAATAGTGGAATAGAATATT

refseq_IL5RA.F2 (forward primer)
refseq_IL5RA.R2 (reverse primer)
Single exon skipping

Frame 0

STHGKLVYLVTMSKS*FPLLKRANQL*KRVKMQ TRQLSDNGYLDERFFRLTAVWLLGVPVQLLHSCS VQILLHICLEKTCCHPIMKEAA*EFLTITAVMM KE*RTASKLKSAPRDKVRSQCPAAASHRMATAF LRPGTLSIGPVPDAMLDAGVAATSIDTTDTVLAI FC*VLVGTRGSSVDRI*SSWRMYYSSFWGPLRY CKLTYFLMKRFHFSHLSISPLKLLVWLKFFYNG NQILIKSKGMLI*NIK*K*TLQKKMTMKPESLKA NV*PSSTKAFQQVCGPSCRTTTHYWPAAGLLLN FMPHQGLLEPQL*I*LAPQTLQKTIIHV*GHTK FPFTAPGLLAQMPLRTRSIFSTIGMALGLKNAKN TAKTHWGEISHAGFPGLLSSAKGVTGLRCLLTA PASTLLSGPLISCLPFTPLIK*ILH*MSQQRLK ELVSLSNGRNQCLLFQSIALIMK*KYTIQGMDIC R*KN**PMHSSQ*LMIFLSTMFK*EQQ*APCAE RQGSGVSGANLFMWVSSLCLFYIGSLPCDQKR* SQKRTRSLVGLKMVSYLYCD*FSLGLLPFQSGID LP*SQ*SF*VSRLLISANSPRPGACFFSKFCFY FYPFFKLNFLF*NNYHTHKLWEEIIEILCLFHP VFLKGNILQNYSTIVE*NI

Frame 1

VPTENLFTLSP*VKVNSHS*REQTNSERE*KCR QDSYQIMAIWTRDSFV*QQFGCWEFQFSSCTVA LYKSSSIFA*RKRVAIPS*RKLPESF*PLQP*** KSEEPPLS*KVHPEIRFVLNALPLLLIAWPPHF SGQAH*ALVLCLTLC*MLGLQPRA*TRQTRSSP SSVEYWSEQEDRL*TGYDHRGACITHPFGGH*DT AS*LTS**KDFTSPTCQFHH*SYWFGSSSFTME TKS*SRAKEC*SRISSENKRSKRR*L*NQNH*K QMCNHPPQRLFSKCADHPAERPLTTGQQLGFC*T SCPTRVSWNLNCEFNLHHKHYRRQLFTFKVIPS FPSLHLACWHRCP*GHAVFSLL*VWLLD*RMPR IQQRHTGEKYRMLVSQDFYPQQRA*LACGAC*RL QQALCYQAL*SAVCPSRH*SNKSSTECHSRD*R NSSLYPMGETSVCFSNPLL*L*SKNTQYKEWIF ADRKIDDQCIHLNN**SF*VRCSSESSSELHVQR GRALE*VEPTYLCG*VAYVYFTLAAFLVIKKGN PRNVPVHSWVLKWFHISIVTNFLSVYCLFNQE* ICHEASEVFKCLGFSLAPTLLDLVPVFFPSFVST SIHFLN*TFYFEIIITLTSCGKK**RSCVSFIQ FSSRVTSYKTIVQ*WNRI

Frame 2

YPRKTCLPCHHE*KLIPTPEESKPTLKESENAD KTVIR*WLSGREILSFDSSLVVGSSSSAPAQLL CTNPPPYLLRENVLPSHHEGSCLRVFNHYSRDD ERVKNRL*VKKCTQR*GSFSMPCRCFSSHGHRIS QARHIEHWSCA*RYARCWGCSHEHRHDRHGPRH LLLSTGRNKRIVCRQDMIIVAHVLLILLGATEI LQADLLPDEKISLLPPVNFTIKVTGLAQVLLQWK PNPDQEQRNVNLEYQVKINAPKEDDYETRITES KCVTILHKGFSASVRTILQNDHSLLASSWASAE LHAPPGSPGTSIVNLTCTTNTTEDNYSRLRSYQV SLHCTWLVGTDAPEDTQYFLYYRYGSWTEECQE YSKDTLGRNIACWFPRTFILSKGRDWLAVLVNG SSKHSAIRPFDQLFALHAIDQINPPLNVTAEIEG TRLSIQWEKPVSAFPIHCFDYEVKIHNTRNGYL QIEKLMTNAFISIIDDLSKYDVQVRAAVSSMCR EAGLWSEWSQPIYVGK*LMFILHWQPSL*SKKVI PETYPFTRGS*NGFISLL*LIFSRSTAFSIRNR FAMKPVKFLSV*ASH*RQLS*TWCLFFFQVLFL LLSIF*IKLFILK*LSHSQAVGRNNRDPVSLSSS FPQG*HLTKL*YNSGIEY

Sequence NM_175725

AGTACCCACGGAAAACTTGTTTACCTTGTCACCATGAGTAAAAGTTAATTCCCACTCCTGAAGAGAGCAAACCAACTCTGAAAGAGAGTGAAAATGCAGA CAAGACAGTTATCAGATAATGGCTATCTGGACGAGAGATTCTTTCGTTTGACAGCAGTTTGGTTGTTGGGAGTTCCAGTTCAGCTCCTGCACAGTTGCTC TGTACAAATCCTCCTCCATATTTGCTTAGAGAAAACGTGTTGCCATCCCATCATGAAGGAAGCTGCCTGAGAGTTTTTAACCATTACAGCCGTGATGATG AAAGAGTGAAGAACCGCCTCTAAGTTAAAAAGTGCACCCAGAGATAAGGTTCGTTCTCAATGCCCTGCCGCTGCTTCTCATCGCATGGCCACCGCATTTC TCAGGCCAGGCACATTGAGCATTGGTCCTGTGCCTGACGCTATGCTAGATGCTGGGGTTGCAGCCACGAGCATAGACACGACAGACACGGTCCTCGCCAT CTTCTGTTGAGTACTGGTCGGAACAAGAGGATCGTCTGTAGACAGGCTACAGATTGTTTTAGATTGAAGTTTCCTGTCATGTTCACTCATCTTTAAATCC TCATAGTGAAAAAGGATATGATCATCGTGGCGCATGTATTACTCATCCTTTTGGGGGCCACTGAGATACTGCAAGCTGACTTACTTCCTGATGAAAAGAT TTCACTTCTCCCACCTGTCAATTTCACCATTAAAGTTACTGGTTTGGCTCAAGTTCTTTTACAATGGAAACCAAATCCTGATCAAGAGCAAAGGAATGTT AATCTAGAATATCAAGTGAAAATAAACGCTCCAAAAGAAGATGACTATGAAACCAGAATCACTGAAAGCAAATGTGTAACCATCCTCCACAAAGGCTTTT CAGCAAGTGTGCGGACCATCCTGCAGAACGACCACTCACTACTGGCCAGCAGCTGGGCTTCTGCTGAACTTCATGCCCCACCAGGGTCTCCTGGAACCTC AATTGTGAATTTAACTTGCACCACAAACACTACAGAAGACAATTATTCACGTTTAAGGTCATACCAAGTTTCCCTTCACTGCACCTGGCTTGTTGGCACA GATGCCCCTGAGGACACGCAGTATTTTCTCTACTATAGGTATGGCTCTTGGACTGAAGAATGCCAAGAATACAGCAAAGACACACTGGGGAGAAATATCG CATGCTGGTTTCCCAGGACTTTTATCCTCAGCAAAGGGCGTGACTGGCTTGCGGTGCTTGTTAACGGCTCCAGCAAGCACTCTGCTATCAGGCCCTTTGA TCAGCTGTTTGCCCTTCACGCCATTGATCAAATAAATCCTCCACTGAATGTCACAGCAGAGATTGAAGGAACTCGTCTCTCTATCCAATGGGAGAAACCA GTGTCTGCTTTTCCAATCCATTGCTTTGATTATGAAGTAAAAATACACAATACAAGGAATGGATATTTGCAGATAGAAAAATTGATGACCAATGCATTCA TCTCAATAATTGATGATCTTTCTAAGTACGATGTTCAAGTGAGAGCAGCAGTGAGCTCCATGTGCAGAGAGGCAGGGCTCTGGAGTGAGTGGAGCCAACC TATTTATGTGGGGTTCTCAAGATAAAGGAGATAACATCCAGCTTTCCTGCCCCACACCGTATCTGAAATAAAAACAACAGCAGGGATAGCAGATT

refseq_IL5RA.F2 (forward primer)
refseq_IL5RA.R2 (reverse primer)
Single exon skipping

Frame 0

STHGKLVYLVTMSKS*FPLLKRANQL*KRVKMQ TRQLSDNGYLDERFFRLTAVWLLGVPVQLLHSCS VQILLHICLEKTCCHPIMKEAA*EFLTITAVMM KE*RTASKLKSAPRDKVRSQCPAAASHRMATAF LRPGTLSIGPVPDAMLDAGVAATSIDTTDTVLAI FC*VLVGTRGSSVDRLQIVLD*SFLSCSLIFKS S**KRI*SSWRMYYSSFWGPLRYCKLTYFLMKR FHFSHLSISPLKLLVWLKFFYNGNQILIKSKGML I*NIK*K*TLQKKMTMKPESLKANV*PSSTKAF QQVCGPSCRTTTHYWPAAGLLLNFMPHQGLLEP QL*I*LAPQTLQKTIIHV*GHTKFPFTAPGLLAQ MPLRTRSIFSTIGMALGLKNAKNTAKTHWGEIS HAGFPGLLSSAKGVTGLRCLLTAPASTLLSGPL ISCLPFTPLIK*ILH*MSQQRLKELVSLSNGRNQ CLLFQSIALIMK*KYTIQGMDICR*KN**PMHS SQ*LMIFLSTMFK*EQQ*APCAERQGSGVSGAN LFMWGSQDKGDNIQLSCPTPYLK*KQQQG*QI

Frame 1

VPTENLFTLSP*VKVNSHS*REQTNSERE*KCR QDSYQIMAIWTRDSFV*QQFGCWEFQFSSCTVA LYKSSSIFA*RKRVAIPS*RKLPESF*PLQP*** KSEEPPLS*KVHPEIRFVLNALPLLLIAWPPHF SGQAH*ALVLCLTLC*MLGLQPRA*TRQTRSSP SSVEYWSEQEDRL*TGYRLF*IEVSCHVHSSLNP HSEKGYDHRGACITHPFGGH*DTAS*LTS**KD FTSPTCQFHH*SYWFGSSSFTMETKS*SRAKEC *SRISSENKRSKRR*L*NQNH*KQMCNHPPQRLF SKCADHPAERPLTTGQQLGFC*TSCPTRVSWNL NCEFNLHHKHYRRQLFTFKVIPSFPSLHLACWH RCP*GHAVFSLL*VWLLD*RMPRIQQRHTGEKYR MLVSQDFYPQQRA*LACGAC*RLQQALCYQAL* SAVCPSRH*SNKSSTECHSRD*RNSSLYPMGET SVCFSNPLL*L*SKNTQYKEWIFADRKIDDQCIH LNN**SF*VRCSSESSSELHVQRGRALE*VEPT YLCGVLKIKEITSSFPAPHRI*NKNNSRDSR

Frame 2

YPRKTCLPCHHE*KLIPTPEESKPTLKESENAD KTVIR*WLSGREILSFDSSLVVGSSSSAPAQLL CTNPPPYLLRENVLPSHHEGSCLRVFNHYSRDD ERVKNRL*VKKCTQR*GSFSMPCRCFSSHGHRIS QARHIEHWSCA*RYARCWGCSHEHRHDRHGPRH LLLSTGRNKRIVCRQATDCFRLKFPVMFTHL*I LIVKKDMIIVAHVLLILLGATEILQADLLPDEKI SLLPPVNFTIKVTGLAQVLLQWKPNPDQEQRNV NLEYQVKINAPKEDDYETRITESKCVTILHKGF SASVRTILQNDHSLLASSWASAELHAPPGSPGTS IVNLTCTTNTTEDNYSRLRSYQVSLHCTWLVGT DAPEDTQYFLYYRYGSWTEECQEYSKDTLGRNI ACWFPRTFILSKGRDWLAVLVNGSSKHSAIRPFD QLFALHAIDQINPPLNVTAEIEGTRLSIQWEKP VSAFPIHCFDYEVKIHNTRNGYLQIEKLMTNAF ISIIDDLSKYDVQVRAAVSSMCREAGLWSEWSQP IYVGFSR*RR*HPAFLPHTVSEIKTTAGIAD

Sequence NM_000564

AGTACCCACGGAAAACTTGTTTACCTTGTCACCATGAGTAAAAGTTAATTCCCACTCCTGAAGAGAGCAAACCAACTCTGAAAGAGAGTGAAAATGCAGA CAAGACAGTTATCAGATAATGGCTATCTGGACGAGAGATTCTTTCGTTTGACAGCAGTTTGGTTGTTGGGAGTTCCAGTTCAGCTCCTGCACAGTTGCTC TGTACAAATCCTCCTCCATATTTGCTTAGAGAAAACGTGTTGCCATCCCATCATGAAGGAAGCTGCCTGAGAGTTTTTAACCATTACAGCCGTGATGATG AAAGAGTGAAGAACCGCCTCTAAGTTAAAAAGTGCACCCAGAGATAAGGTTCGTTCTCAATGCCCTGCCGCTGCTTCTCATCGCATGGCCACCGCATTTC TCAGGCCAGGCACATTGAGCATTGGTCCTGTGCCTGACGCTATGCTAGATGCTGGGGTTGCAGCCACGAGCATAGACACGACAGACACGGTCCTCGCCAT CTTCTGTTGAGTACTGGTCGGAACAAGAGGATCGTCTGTAGACAGGCTACAGATTGTTTTAGATTGAAGTTTCCTGTCATGTTCACTCATCTTTAAATCC TCATAGTGAAAAAGGATATGATCATCGTGGCGCATGTATTACTCATCCTTTTGGGGGCCACTGAGATACTGCAAGCTGACTTACTTCCTGATGAAAAGAT TTCACTTCTCCCACCTGTCAATTTCACCATTAAAGTTACTGGTTTGGCTCAAGTTCTTTTACAATGGAAACCAAATCCTGATCAAGAGCAAAGGAATGTT AATCTAGAATATCAAGTGAAAATAAACGCTCCAAAAGAAGATGACTATGAAACCAGAATCACTGAAAGCAAATGTGTAACCATCCTCCACAAAGGCTTTT CAGCAAGTGTGCGGACCATCCTGCAGAACGACCACTCACTACTGGCCAGCAGCTGGGCTTCTGCTGAACTTCATGCCCCACCAGGGTCTCCTGGAACCTC AATTGTGAATTTAACTTGCACCACAAACACTACAGAAGACAATTATTCACGTTTAAGGTCATACCAAGTTTCCCTTCACTGCACCTGGCTTGTTGGCACA GATGCCCCTGAGGACACGCAGTATTTTCTCTACTATAGGTATGGCTCTTGGACTGAAGAATGCCAAGAATACAGCAAAGACACACTGGGGAGAAATATCG CATGCTGGTTTCCCAGGACTTTTATCCTCAGCAAAGGGCGTGACTGGCTTGCGGTGCTTGTTAACGGCTCCAGCAAGCACTCTGCTATCAGGCCCTTTGA TCAGCTGTTTGCCCTTCACGCCATTGATCAAATAAATCCTCCACTGAATGTCACAGCAGAGATTGAAGGAACTCGTCTCTCTATCCAATGGGAGAAACCA GTGTCTGCTTTTCCAATCCATTGCTTTGATTATGAAGTAAAAATACACAATACAAGGAATGGATATTTGCAGATAGAAAAATTGATGACCAATGCATTCA TCTCAATAATTGATGATCTTTCTAAGTACGATGTTCAAGTGAGAGCAGCAGTGAGCTCCATGTGCAGAGAGGCAGGGCTCTGGAGTGAGTGGAGCCAACC TATTTATGTGGGAAATGATGAACACAAGCCCTTGAGAGAGTGGTTTGTCATTGTGATTATGGCAACCATCTGCTTCATCTTGTTAATTCTCTCGCTTATC TGTAAAATATGTCATTTATGGATCAAGTTGTTTCCACCAATTCCAGCACCAAAAAGTAATATCAAAGATCTCTTTGTAACCACTAACTATGAGAAAGCTG GGTCCAGTGAGACGGAAATTGAAGTCATCTGTTATATAGAGAAGCCTGGAGTTGAGACCCTGGAGGATTCTGTGTTTTGACTGTCACTTTGGCATCCTCT GATGAACTCACACATGCCTCAGTGCCTCAGTGAAAAGAACAGGGATGCTGGCTCTTGGCTAAGAGGTGTTCAGAATTTAGGCAACACTCAATTTACCTGC GAAGCAATACACCCAGACACACCAGTCTTGTATCTCTTAAAAGTATGGATGCTTCATCCAAATCGCCTCACCTACAGCAGGGAAGTTGACTCATCCAAGC ATTTTGCCATGTTTTTTCTCCCCATGCCGTACAGGGTAGCACCTCCTCACCTGCCAATCTTTGCAATTTGCTTGACTCACCTCAGACTTTTCATTCACAA CAGACAGCTTTTAAGGCTAACGTCCAGCTGTATTTACTTCTGGCTGTGCCCGTTTGGCTGTTTAAGCTGCCAATTGTAGCACTCAGCTACCATCTGAGGA AGAAAGCATTTTGCATCAGCCTGGAGTGAACCATGAACTTGGATTCAAGACTGTCTTTTCTATAGCAA

refseq_IL5RA.F2 (forward primer)
refseq_IL5RA.R2 (reverse primer)
Single exon skipping

Frame 0

STHGKLVYLVTMSKS*FPLLKRANQL*KRVKMQ TRQLSDNGYLDERFFRLTAVWLLGVPVQLLHSCS VQILLHICLEKTCCHPIMKEAA*EFLTITAVMM KE*RTASKLKSAPRDKVRSQCPAAASHRMATAF LRPGTLSIGPVPDAMLDAGVAATSIDTTDTVLAI FC*VLVGTRGSSVDRLQIVLD*SFLSCSLIFKS S**KRI*SSWRMYYSSFWGPLRYCKLTYFLMKR FHFSHLSISPLKLLVWLKFFYNGNQILIKSKGML I*NIK*K*TLQKKMTMKPESLKANV*PSSTKAF QQVCGPSCRTTTHYWPAAGLLLNFMPHQGLLEP QL*I*LAPQTLQKTIIHV*GHTKFPFTAPGLLAQ MPLRTRSIFSTIGMALGLKNAKNTAKTHWGEIS HAGFPGLLSSAKGVTGLRCLLTAPASTLLSGPL ISCLPFTPLIK*ILH*MSQQRLKELVSLSNGRNQ CLLFQSIALIMK*KYTIQGMDICR*KN**PMHS SQ*LMIFLSTMFK*EQQ*APCAERQGSGVSGAN LFMWEMMNTSP*ESGLSL*LWQPSASSC*FSRLS VKYVIYGSSCFHQFQHQKVISKISL*PLTMRKL GPVRRKLKSSVI*RSLELRPWRILCFDCHFGIL **THTCLSASVKRTGMLALG*EVFRI*ATLNLPA KQYTQTHQSCIS*KYGCFIQIASPTAGKLTHPS ILPCFFSPCRTG*HLLTCQSLQFA*LTSDFSFT TDSF*G*RPAVFTSGCARLAV*AANCSTQLPSEE ESILHQPGVNHELGFKTVFSIA

Frame 1

VPTENLFTLSP*VKVNSHS*REQTNSERE*KCR QDSYQIMAIWTRDSFV*QQFGCWEFQFSSCTVA LYKSSSIFA*RKRVAIPS*RKLPESF*PLQP*** KSEEPPLS*KVHPEIRFVLNALPLLLIAWPPHF SGQAH*ALVLCLTLC*MLGLQPRA*TRQTRSSP SSVEYWSEQEDRL*TGYRLF*IEVSCHVHSSLNP HSEKGYDHRGACITHPFGGH*DTAS*LTS**KD FTSPTCQFHH*SYWFGSSSFTMETKS*SRAKEC *SRISSENKRSKRR*L*NQNH*KQMCNHPPQRLF SKCADHPAERPLTTGQQLGFC*TSCPTRVSWNL NCEFNLHHKHYRRQLFTFKVIPSFPSLHLACWH RCP*GHAVFSLL*VWLLD*RMPRIQQRHTGEKYR MLVSQDFYPQQRA*LACGAC*RLQQALCYQAL* SAVCPSRH*SNKSSTECHSRD*RNSSLYPMGET SVCFSNPLL*L*SKNTQYKEWIFADRKIDDQCIH LNN**SF*VRCSSESSSELHVQRGRALE*VEPT YLCGK**TQALERVVCHCDYGNHLLHLVNSLAY L*NMSFMDQVVSTNSSTKK*YQRSLCNH*L*ESW VQ*DGN*SHLLYREAWS*DPGGFCVLTVTLASS DELTHASVPQ*KEQGCWLLAKRCSEFRQHSIYL RSNTPRHTSLVSLKSMDASSKSPHLQQGS*LIQA FCHVFSPHAVQGSTSSPANLCNLLDSPQTFHSQ QTAFKANVQLYLLLAVPVWLFKLPIVALSYHLR KKAFCISLE*TMNLDSRLSFL*Q

Frame 2

YPRKTCLPCHHE*KLIPTPEESKPTLKESENAD KTVIR*WLSGREILSFDSSLVVGSSSSAPAQLL CTNPPPYLLRENVLPSHHEGSCLRVFNHYSRDD ERVKNRL*VKKCTQR*GSFSMPCRCFSSHGHRIS QARHIEHWSCA*RYARCWGCSHEHRHDRHGPRH LLLSTGRNKRIVCRQATDCFRLKFPVMFTHL*I LIVKKDMIIVAHVLLILLGATEILQADLLPDEKI SLLPPVNFTIKVTGLAQVLLQWKPNPDQEQRNV NLEYQVKINAPKEDDYETRITESKCVTILHKGF SASVRTILQNDHSLLASSWASAELHAPPGSPGTS IVNLTCTTNTTEDNYSRLRSYQVSLHCTWLVGT DAPEDTQYFLYYRYGSWTEECQEYSKDTLGRNI ACWFPRTFILSKGRDWLAVLVNGSSKHSAIRPFD QLFALHAIDQINPPLNVTAEIEGTRLSIQWEKP VSAFPIHCFDYEVKIHNTRNGYLQIEKLMTNAF ISIIDDLSKYDVQVRAAVSSMCREAGLWSEWSQP IYVGNDEHKPLREWFVIVIMATICFILLILSLI CKICHLWIKLFPPIPAPKSNIKDLFVTTNYEKA GSSETEIEVICYIEKPGVETLEDSVF*LSLWHPL MNSHMPQCLSEKNRDAGSWLRGVQNLGNTQFTC EAIHPDTPVLYLLKVWMLHPNRLTYSREVDSSK HFAMFFLPMPYRVAPPHLPIFAICLTHLRLFIHN RQLLRLTSSCIYFWLCPFGCLSCQL*HSATI*G RKHFASAWSEP*TWIQDCLFYS

Sequence NM_175724

AGTACCCACGGAAAACTTGTTTACCTTGTCACCATGAGTAAAAGTTAATTCCCACTCCTGAAGAGAGCAAACCAACTCTGAAAGAGAGTGAAAATGCAGA CAAGACAGTTATCAGATAATGGCTATCTGGACGAGAGATTCTTTCGTTTGACAGCAGTTTGGTTGTTGGGAGTTCCAGTTCAGCTCCTGCACAGTTGCTC TGTACAAATCCTCCTCCATATTTGCTTAGAGAAAACGTGTTGCCATCCCATCATGAAGGAAGCTGCCTGAGAGTTTTTAACCATTACAGCCGTGATGATG AAAGAGTGAAGAACCGCCTCTAAGTTAAAAAGTGCACCCAGAGATAAGGTTCGTTCTCAATGCCCTGCCGCTGCTTCTCATCGCATGGCCACCGCATTTC TCAGGCCAGGCACATTGAGCATTGGTCCTGTGCCTGACGCTATGCTAGATGCTGGGGTTGCAGCCACGAGCATAGACACGACAGACACGGTCCTCGCCAT CTTCTGTTGAGTACTGGTCGGAACAAGAGGATCGTCTGTAGACAGGCTACAGATTGTTTTAGATTGAAGTTTCCTGTCATGTTCACTCATCTTTAAATCC TCATAGTGAAAAAGGATATGATCATCGTGGCGCATGTATTACTCATCCTTTTGGGGGCCACTGAGATACTGCAAGCTGACTTACTTCCTGATGAAAAGAT TTCACTTCTCCCACCTGTCAATTTCACCATTAAAGTTACTGGTTTGGCTCAAGTTCTTTTACAATGGAAACCAAATCCTGATCAAGAGCAAAGGAATGTT AATCTAGAATATCAAGTGAAAATAAACGCTCCAAAAGAAGATGACTATGAAACCAGAATCACTGAAAGCAAATGTGTAACCATCCTCCACAAAGGCTTTT CAGCAAGTGTGCGGACCATCCTGCAGAACGACCACTCACTACTGGCCAGCAGCTGGGCTTCTGCTGAACTTCATGCCCCACCAGGGTCTCCTGGAACCTC AATTGTGAATTTAACTTGCACCACAAACACTACAGAAGACAATTATTCACGTTTAAGGTCATACCAAGTTTCCCTTCACTGCACCTGGCTTGTTGGCACA GATGCCCCTGAGGACACGCAGTATTTTCTCTACTATAGGTATGGCTCTTGGACTGAAGAATGCCAAGAATACAGCAAAGACACACTGGGGAGAAATATCG CATGCTGGTTTCCCAGGACTTTTATCCTCAGCAAAGGGCGTGACTGGCTTGCGGTGCTTGTTAACGGCTCCAGCAAGCACTCTGCTATCAGGCCCTTTGA TCAGCTGTTTGCCCTTCACGCCATTGATCAAATAAATCCTCCACTGAATGTCACAGCAGAGATTGAAGGAACTCGTCTCTCTATCCAATGGGAGAAACCA GTGTCTGCTTTTCCAATCCATTGCTTTGATTATGAAGTAAAAATACACAATACAAGGAATGGATATTTGCAGATAGAAAAATTGATGACCAATGCATTCA TCTCAATAATTGATGATCTTTCTAAGTACGATGTTCAAGTGAGAGCAGCAGTGAGCTCCATGTGCAGAGAGGCAGGGCTCTGGAGTGAGTGGAGCCAACC TATTTATGTGGGTAAGTAGCTTATGTTTATTTTACATTGGCAGCCTTCCTTGTGATCAAAAAAGGTAATCCCAGAAACGTACCCGTTCACTCGTGGGTCT TAAAATGGTTTCATATCTCTATTGTGACTAATTTTCTCTCGGTCTACTGCCTTTTCAATCAGGAATAGATTTGCCATGAAGCCAGTGAAGTTTTTAAGTG TCTAGGCTTCTCATTAGCGCCAACTCTCCTAGACCTGGTGCCTGTTTTTTTTCCAAGTTTTGTTTCTACTTCTATCCATTTTTTAAATTAAACTTTTTAT TTTGAAATAATTATCACACTCACAAGCTGTGGGAAGAAATAATAGAGATCCTGTGTCTCTTTCATCCAGTTTTCCTCAAGGGTAACATCTTACAAAACTA TAGTACAATAGTGGAATAGAATATT

refseq_IL5RA.F2 (forward primer)
refseq_IL5RA.R2 (reverse primer)
Single exon skipping

Frame 0

STHGKLVYLVTMSKS*FPLLKRANQL*KRVKMQ TRQLSDNGYLDERFFRLTAVWLLGVPVQLLHSCS VQILLHICLEKTCCHPIMKEAA*EFLTITAVMM KE*RTASKLKSAPRDKVRSQCPAAASHRMATAF LRPGTLSIGPVPDAMLDAGVAATSIDTTDTVLAI FC*VLVGTRGSSVDRLQIVLD*SFLSCSLIFKS S**KRI*SSWRMYYSSFWGPLRYCKLTYFLMKR FHFSHLSISPLKLLVWLKFFYNGNQILIKSKGML I*NIK*K*TLQKKMTMKPESLKANV*PSSTKAF QQVCGPSCRTTTHYWPAAGLLLNFMPHQGLLEP QL*I*LAPQTLQKTIIHV*GHTKFPFTAPGLLAQ MPLRTRSIFSTIGMALGLKNAKNTAKTHWGEIS HAGFPGLLSSAKGVTGLRCLLTAPASTLLSGPL ISCLPFTPLIK*ILH*MSQQRLKELVSLSNGRNQ CLLFQSIALIMK*KYTIQGMDICR*KN**PMHS SQ*LMIFLSTMFK*EQQ*APCAERQGSGVSGAN LFMWVSSLCLFYIGSLPCDQKR*SQKRTRSLVGL KMVSYLYCD*FSLGLLPFQSGIDLP*SQ*SF*V SRLLISANSPRPGACFFSKFCFYFYPFFKLNFL F*NNYHTHKLWEEIIEILCLFHPVFLKGNILQNY STIVE*NI

Frame 1

VPTENLFTLSP*VKVNSHS*REQTNSERE*KCR QDSYQIMAIWTRDSFV*QQFGCWEFQFSSCTVA LYKSSSIFA*RKRVAIPS*RKLPESF*PLQP*** KSEEPPLS*KVHPEIRFVLNALPLLLIAWPPHF SGQAH*ALVLCLTLC*MLGLQPRA*TRQTRSSP SSVEYWSEQEDRL*TGYRLF*IEVSCHVHSSLNP HSEKGYDHRGACITHPFGGH*DTAS*LTS**KD FTSPTCQFHH*SYWFGSSSFTMETKS*SRAKEC *SRISSENKRSKRR*L*NQNH*KQMCNHPPQRLF SKCADHPAERPLTTGQQLGFC*TSCPTRVSWNL NCEFNLHHKHYRRQLFTFKVIPSFPSLHLACWH RCP*GHAVFSLL*VWLLD*RMPRIQQRHTGEKYR MLVSQDFYPQQRA*LACGAC*RLQQALCYQAL* SAVCPSRH*SNKSSTECHSRD*RNSSLYPMGET SVCFSNPLL*L*SKNTQYKEWIFADRKIDDQCIH LNN**SF*VRCSSESSSELHVQRGRALE*VEPT YLCG*VAYVYFTLAAFLVIKKGNPRNVPVHSWV LKWFHISIVTNFLSVYCLFNQE*ICHEASEVFKC LGFSLAPTLLDLVPVFFPSFVSTSIHFLN*TFY FEIIITLTSCGKK**RSCVSFIQFSSRVTSYKT IVQ*WNRI

Frame 2

YPRKTCLPCHHE*KLIPTPEESKPTLKESENAD KTVIR*WLSGREILSFDSSLVVGSSSSAPAQLL CTNPPPYLLRENVLPSHHEGSCLRVFNHYSRDD ERVKNRL*VKKCTQR*GSFSMPCRCFSSHGHRIS QARHIEHWSCA*RYARCWGCSHEHRHDRHGPRH LLLSTGRNKRIVCRQATDCFRLKFPVMFTHL*I LIVKKDMIIVAHVLLILLGATEILQADLLPDEKI SLLPPVNFTIKVTGLAQVLLQWKPNPDQEQRNV NLEYQVKINAPKEDDYETRITESKCVTILHKGF SASVRTILQNDHSLLASSWASAELHAPPGSPGTS IVNLTCTTNTTEDNYSRLRSYQVSLHCTWLVGT DAPEDTQYFLYYRYGSWTEECQEYSKDTLGRNI ACWFPRTFILSKGRDWLAVLVNGSSKHSAIRPFD QLFALHAIDQINPPLNVTAEIEGTRLSIQWEKP VSAFPIHCFDYEVKIHNTRNGYLQIEKLMTNAF ISIIDDLSKYDVQVRAAVSSMCREAGLWSEWSQP IYVGK*LMFILHWQPSL*SKKVIPETYPFTRGS *NGFISLL*LIFSRSTAFSIRNRFAMKPVKFLS V*ASH*RQLS*TWCLFFFQVLFLLLSIF*IKLFI LK*LSHSQAVGRNNRDPVSLSSSFPQG*HLTKL *YNSGIEY