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Sequences

Sequence NM_199335

CCGCAGTTCTTGAGTTCCACATGCAGAGCAGATGCGACAGCTAGAAGTGAGTAGGGCCCAGACCCTGGCCCAGGAAGATCCACTAAAGGAGGCCATCCTT CCGCCTTCTTCTGCAGGAGTCAGGATGGAAAGGCAGATGTAAAGTCCCTCATGGCGAAATATAACACGGGGGGCAACCCGACAGAGGATGTCTCAGTCAA TAGCCGACCCTTCAGAGTCACAGGGCCAAACTCATCTTCAGGAATACAAGCAAGAAAGAACTTATTCAACAACCAAGGAAATGCCAGCCCTCCTGCAGGA CCCAGCAATGTACCTAAGTTTGGGTCCCCAAAGCCACCTGTGGCAGTCAAACCTTCTTCTGAGGAAAAGCCTGACAAGGAACCCAAGCCCCCGTTTCTAA AGCCCACTGGAGCAGGCCAAAGATTCGGAACACCAGCCAGCTTGACCACCAGAGACCCCGAGGCGAAAGTGGGATTTCTGAAACCTGTAGGCCCCAAGCC CATCAACTTGCCCAAAGAAGATTCCAAACCTACATTTCCCTGGCCTCCTGGAAACAAGCCATCTCTTCACAGTGTAAACCAAGACCATGACTTAAAGCCA CTAGGCCCGAAATCTGGGCCTACTCCTCCAACCTCAGAAAATGAACAGAAGCAAGCGTTTCCCAAATTGACTGGGGTTAAAGGGAAATTTATGTCAGCAT CACAAGATCTTGAACCCAAGCCCCTCTTCCCCAAACCCGCCTTTGGCCAGAAGCCGCCCCTAAGTACCGAGAACTCCCATGAAGACGAAAGCCCCATGAA GAATGTGTCTTCATCAAAAGGGTCCCCAGCTCCCCTGGGAGTCAGGTCCAAAAGCGGCCCTTTAAAACCAGCAAGGGAAGACTCAGAAAATAAAGACCAT GCAGGGGAGATTTCAAGTTTGCCCTTTCCTGGAGTGGTTTTGAAACCTGCTGCGAGCAGGGGAGGCCCAGGTCTCTCCAAAAATGGTGAAGAAAAAAAGG AAGATAGGAAGATAGATGCTGCTAAGAACACCTTCCAGAGCAAAATAAATCAGGAAGAGTTGGCCTCAGGGACTCCTCCTGCCAGGTTCCCTAAGGCCCC TTCTAAGCTGACAGTGGGGGGGCCATGGGGCCAAAGTCAGGAAAAGGAAAAGGGAGACAAGAATTCAGCCACCCCGAAACAGAAGCCATTGCCTCCCTTG TTTACCTTGGGTCCACCTCCACCAAAACCCAACAGACCACCAAATGTTGACCTGACGAAATTCCACAAAACCTCTTCTGGAAACAGTACTAGCAAAGGCC AGACGTCTTACTCAACAACTTCCCTGCCACCACCTCCACCATCCCATCCGGCCAGCCAACCACCATTGCCAGCATCTCACCCATCACAACCACCAGTCCC AAGCCTACCTCCCAGAAACATTAAACCTCCGTTTGACCTAAAAAGCCCTGTCAATGAAGACAATCAAGATGGTGTCACGCACTCTGATGGTGCTGGAAAT CTAGATGAGGAACAAGACAGTGAAGGAGAAACATATGAAGACATAGAAGCATCCAAAGAAAGAGAGAAGAAAAGGGAAAAGGAAGAAAAGAAGAGGTTAG AGCTGGAGAAAAAGGAACAGAAAGAGAAAGAAAAGAAAGAACAAGAAATAAAGAAGAAATTTAAACTAACAGGCCCTATTCAAGTCATCCATCTTGCAAA AGCTTGTTGTGATGTCAAAGGAGGAAAGAATGAACTGAGCTTCAAGCAAGGAGAGCAAATTGAAATCATCCGCATCACAGACAACCCAGAAGGAAAATGG TTGGGCAGAACAGCAAGGGGTTCATATGGCTATATTAAAACAACTGCTGTAGAGATTGACTATGATTCTTTGAAACTGAAAAAAGACTCTCTTGGTGCCC CTTCAAGACCTATTGAAGATGACCAAGAAGTATATGATGATGTTGCAGAGCAGGATGATATTAGCAGCCACAGTCAGAGTGGAAGTGGAGGGATATTCCC TCCACCACCAGATGATGACATTTATGATGGGATTGAAGAGGAAGATGCTGATGATGGTTTCCCTGCTCCTCCTAAACAATTGGACATGGGAGATGAAGTT TACGATGATGTGGATACCTCTGATTTCCCTGTTTCATCAGCAGAGATGAGTCAAGGAACTAATGTTGGAAAAGCTAAGACAGAAGAAAAGGACCTTAAGA AGCTAAAAAAGCAGGAAAAAGAAGAAAAAGACTTCAGGAAAAAATTTAAATATGATGGTGAAATTAGAGTCCTATATTCAACTAAAGTTACAACTTCCAT AACTTCTAAAAAGTGGGGAACCAGAGATCTACAGGTAAAACCTGGTGAATCTCTAGAAGTTATACAAACCACAGATGACACAAAAGTTCTCTGCAGAAAT GAAGAAGGGAAATATGGTTATGTCCTTCGGAGTTACCTAGCGGACAATGATGGAGAGATCTATGATGATATTGCTGATGGCTGCATCTATGACAATGACT AGCACTCAACTTTGGTCATTCTGCTGTGTTCATTAGGTGCCAATGTGAAGTCTGGATTTTAATTGGCATGTTATTGGGTATCAAGAAAATTAATGCACAA AACCACTTATTATCATTTGTTATGAAATCCCAATTATCTTTACAAAGTGTTTAAAGTTTGAACATAGAAAATAATCTCTCTGCTTAATTGTTAACTCAGA AGACTACATTAGTGAGATGTAAGAATTATTAAATATTCCATTTCCGCTTTGGCTACAATTATGAAGAAGTTGAAGGTACTTCTTTTAGACCACCAGTAAA TAATCCTCCTTCAAAAAATAAAAATAAAAGAAAAAGGAAAATCATTCAGGAAGAAATGACCTGTCTAAAAAAACCTAAGGAAGAATAATAATATAAGAAA GGAAATTTAAAAACATTCCACAAGAAGAAAAATTATTGTTTATACTTCTACTTATGGTTATATCTTATATTCTCTATTCAAGTGACCTGTCTTTTAAAAA GGCAGTGCTGTCTTACCTCTTGCTAGTGGGTTAAATGTTTTCAAAAATTATAGCAGTAGTAGAAGTTTTGTATAAAATTTGTCCTTATTTGTTAATTGTA TATAAATGTTAATTATTTGATACGAATGTTATGCATTTAGTATGCACATTGAAGTCTAAACTGTAGAAGAGTCTAAAACAAGTTCTCTTTTTGCAGATTC ACATACTAATGGTTTAATTCTGTGCTCTGTTTAAAGTACTATTATAACTAGAGTAGATCTGAATGAGGATAACCCTAAAATCATGAGGAATGGAAGAATG GACCTTGAAACTACCTAGGCTTTTATGCATGGCACCTCTTTATAATGAAGACACTTTTTAAAGTTTTTGTTTTTGTTTCAATTACCGCTAGATTTTTTTT TCTCTTTTTTTAAAATCCATTTTACTGGAAAGTTGGCCAGCAGAGGGAGTAGAAATTATTAAAATTCTAGTGTTTGGATTGGGCCCTTCTCTAACAGTAC ATACTCATTCCCAAAGCAATCCAAAAACAAAATGTGAACCATTTGGGTTTCAAATGTTAAGAACACTAAATAGCATGATTTAAAAAATGAAAAATGCTAA CACCCAAGAAAAGAAGATATTAAGTGCTTTTTAACAACTCCTAGAGTACAAAATGAGTACATCATAATGCTGGCTCTTCTACTAATGAACCATCGAGTGA TATTGAATAAATTATTTATCTTCTCAGTTTCCTTATCTGTAAATTACAATATTAGACTAAGTAAGTTTTTCCAACTCTTCACTACCAATTACCTTAGGCT TTTATAATGCTCCGCCTACTTCAGTCCCATGTTTCAGAAGCTTTTGTCTATTTTTTAAACTCATTGATTAAATAATGATTAATGCATTCTCCACATTTTA ATATTGCAAAGGCCCATTGGAGTTTCTGAAGTGGCTCCACAGAATTGAAATAATTTCAAATAACTGTAAAGGAACTGAAAATCTTCACAGAGATGAAGTG GGGTTTCCATTAGGTGCTTTGAAATTTGATAACAAATCATCAACTTCCACTGGTCAATATATAGATTTTGGGTGTCTGAGGCCCCAAGATTAGATGCCAC TAATCTCCAAAGATTCCCTCCAATTATGAAATATTTTAATGTCTACTTTTAGAGAGCACTAGCCAGTATATGACCATGTGATTAATTTCTTTTCACACTA GATAAAATTACCTGGTTCAAAAGTGGTTTTTGTTTATTAAATTTGGTAATAAATATATATAATACACAGACAGGATAGTTTTTATGCTGAAGTTTTTGGC CAGCTTTAGTTTGAGGACTCCTTGATAAGCTTGCTAAACTTTCAGAGTGCCCTGAGACACTTCCAGCCATCCCTCCTCCTGCCTTCATTGGGGCAGACTT GCATTGCAGTCTGACAGTAATTTTTTTTCTGATTGAGAATTATGTAAATTCAGTACAATGTCAGTTTTTAAAAGTCAAAGTTAGATCAAGAGAATATTTC AGAGTTTTGGTTTACACATCAAGAAACAGACACACATACCTAGGAAAGATTTACACAATAGATAATCATCTTAATGTGAAAGATATTTGAAGTATTAATT TTAATATATTAAATATGATTTCTGTTATAGTCTTCTGTATGGAATTTTGTCACTTAAGATGAGCTGCAAATAAATAATACCTTCAATGGA

refseq_FYB.F1 (forward primer)
refseq_FYB.R1 (reverse primer)
Single exon skipping

Frame 0

PQFLSSTCRADATARSE*GPDPGPGRSTKGGHP SAFFCRSQDGKADVKSLMAKYNTGGNPTEDVSVN SRPFRVTGPNSSSGIQARKNLFNNQGNASPPAG PSNVPKFGSPKPPVAVKPSSEEKPDKEPKPPFL KPTGAGQRFGTPASLTTRDPEAKVGFLKPVGPKP INLPKEDSKPTFPWPPGNKPSLHSVNQDHDLKP LGPKSGPTPPTSENEQKQAFPKLTGVKGKFMSA SQDLEPKPLFPKPAFGQKPPLSTENSHEDESPMK NVSSSKGSPAPLGVRSKSGPLKPAREDSENKDH AGEISSLPFPGVVLKPAASRGGPGLSKNGEEKK EDRKIDAAKNTFQSKINQEELASGTPPARFPKAP SKLTVGGPWGQSQEKEKGDKNSATPKQKPLPPL FTLGPPPPKPNRPPNVDLTKFHKTSSGNSTSKG QTSYSTTSLPPPPPSHPASQPPLPASHPSQPPVP SLPPRNIKPPFDLKSPVNEDNQDGVTHSDGAGN LDEEQDSEGETYEDIEASKEREKKREKEEKKRL ELEKKEQKEKEKKEQEIKKKFKLTGPIQVIHLAK ACCDVKGGKNELSFKQGEQIEIIRITDNPEGKW LGRTARGSYGYIKTTAVEIDYDSLKLKKDSLGA PSRPIEDDQEVYDDVAEQDDISSHSQSGSGGIFP PPPDDDIYDGIEEEDADDGFPAPPKQLDMGDEV YDDVDTSDFPVSSAEMSQGTNVGKAKTEEKDLK KLKKQEKEEKDFRKKFKYDGEIRVLYSTKVTTSI TSKKWGTRDLQVKPGESLEVIQTTDDTKVLCRN EEGKYGYVLRSYLADNDGEIYDDIADGCIYDND *HSTLVILLCSLGANVKSGF*LACYWVSRKLMHK TTYYHLL*NPNYLYKVFKV*T*KIISLLNC*LR RLH**DVRIIKYSISALATIMKKLKVLLLDHQ* IILLQKIKIKEKGKSFRKK*PV*KNLRKNNNIRK EI*KHSTRRKIIVYTSTYGYILYSLFK*PVF*K GSAVLPLASGLNVFKNYSSSRSFV*NLSLFVNC I*MLII*YECYAFSMHIEV*TVEESKTSSLFADS HTNGLILCSV*STIITRVDLNEDNPKIMRNGRM DLETT*AFMHGTSL**RHFLKFLFLFQLPLDFF FSFFKIHFTGKLASRGSRNY*NSSVWIGPFSNST YSFPKQSKNKM*TIWVSNVKNTK*HDLKNEKC* HPRKEDIKCFLTTPRVQNEYIIMLALLLMNHRV ILNKLFIFSVSLSVNYNIRLSKFFQLFTTNYLRL L*CSAYFSPMFQKLLSIF*TH*LNND*CILHIL ILQRPIGVSEVAPQN*NNFK*L*RN*KSSQR*S GVSIRCFEI**QIINFHWSIYRFWVSEAPRLDAT NLQRFPPIMKYFNVYF*RALASI*PCD*FLFTL DKITWFKSGFCLLNLVINIYNTQTG*FLC*SFW PALV*GLLDKLAKLSECPETLPAIPPPAFIGADL HCSLTVIFFLIENYVNSVQCQFLKVKVRSREYF RVLVYTSRNRHTYLGKIYTIDNHLNVKDI*SIN FNILNMISVIVFCMEFCHLR*AANK*YLQW

Frame 1

RSS*VPHAEQMRQLEVSRAQTLAQEDPLKEAIL PPSSAGVRMERQM*SPSWRNITRGATRQRMSQS IADPSESQGQTHLQEYKQERTYSTTKEMPALLQD PAMYLSLGPQSHLWQSNLLLRKSLTRNPSPRF* SPLEQAKDSEHQPA*PPETPRRKWDF*NL*APS PSTCPKKIPNLHFPGLLETSHLFTV*TKTMT*SH *ARNLGLLLQPQKMNRSKRFPN*LGLKGNLCQH HKILNPSPSSPNPPLARSRP*VPRTPMKTKAP* RMCLHQKGPQLPWESGPKAAL*NQQGKTQKIKTM QGRFQVCPFLEWF*NLLRAGEAQVSPKMVKKKR KIGR*MLLRTPSRAK*IRKSWPQGLLLPGSLRP LLS*QWGGHGAKVRKRKRETRIQPPRNRSHCLPC LPWVHLHQNPTDHQMLT*RNSTKPLLETVLAKA RRLTQQLPCHHLHHPIRPANHHCQHLTHHNHQS QAYLPETLNLRLT*KALSMKTIKMVSRTLMVLEI *MRNKTVKEKHMKT*KHPKKERRKGKRKKRRG* SWRKRNRKRKKRKNKK*RRNLN*QALFKSSILQ KLVVMSKEERMN*ASSKESKLKSSASQTTQKENG WAEQQGVHMAILKQLL*RLTMIL*N*KKTLLVP LQDLLKMTKKYMMMLQSRMILAATVRVEVEGYS LHHQMMTFMMGLKRKMLMMVSLLLLNNWTWEMKF TMMWIPLISLFHQQR*VKELMLEKLRQKKRTLR S*KSRKKKKKTSGKNLNMMVKLESYIQLKLQLP *LLKSGEPEIYR*NLVNL*KLYKPQMTQKFSAEM KKGNMVMSFGVT*RTMMERSMMILLMAASMTMT STQLWSFCCVH*VPM*SLDFNWHVIGYQEN*CT KPLIIICYEIPIIFTKCLKFEHRK*SLCLIVNSE DYISEM*ELLNIPFPLWLQL*RS*RYFF*TTSK *SSFKK*K*KKKENHSGRNDLSKKT*GRIII*E RKFKNIPQEEKLLFILLLMVISYILYSSDLSFKK AVLSYLLLVG*MFSKIIAVVEVLYKICPYLLIV YKC*LFDTNVMHLVCTLKSKL*KSLKQVLFLQI HILMV*FCALFKVLL*LE*I*MRITLKS*GMEEW TLKLPRLLCMAPLYNEDTF*SFCFCFNYR*IFF SLFLKSILLESWPAEGVEIIKILVFGLGPSLTV HTHSQSNPKTKCEPFGFQMLRTLNSMI*KMKNAN TQEKKILSAF*QLLEYKMSTS*CWLFY**TIE* Y*INYLSSQFPYL*ITILD*VSFSNSSLPITLG FYNAPPTSVPCFRSFCLFFKLID*IMINAFSTF* YCKGPLEFLKWLHRIEIISNNCKGTENLHRDEV GFPLGALKFDNKSSTSTGQYIDFGCLRPQD*MP LISKDSLQL*NILMSTFREH*PVYDHVINFFSH* IKLPGSKVVFVY*IW**IYIIHRQDSFYAEVFG QL*FEDSLISLLNFQSALRHFQPSLLLPSLGQT CIAV*Q*FFF*LRIM*IQYNVSF*KSKLDQENIS EFWFTHQETDTHT*ERFTQ*IIILM*KIFEVLI LIY*I*FLL*SSVWNFVT*DELQINNTFNG

Frame 2

AVLEFHMQSRCDS*K*VGPRPWPRKIH*RRPSF RLLLQESGWKGRCKVPHGEI*HGGQPDRGCLSQ *PTLQSHRAKLIFRNTSKKELIQQPRKCQPSCR TQQCT*VWVPKATCGSQTFF*GKA*QGTQAPVSK AHWSRPKIRNTSQLDHQRPRGESGISETCRPQA HQLAQRRFQTYISLASWKQAISSQCKPRP*LKA TRPEIWAYSSNLRK*TEASVSQIDWG*REIYVSI TRS*TQAPLPQTRLWPEAAPKYRELP*RRKPHE ECVFIKRVPSSPGSQVQKRPFKTSKGRLRK*RP CRGDFKFALSWSGFETCCEQGRPRSLQKW*RKKG R*EDRCC*EHLPEQNKSGRVGLRDSSCQVP*GP F*ADSGGAMGPKSGKGKGRQEFSHPETEAIASL VYLGSTSTKTQQTTKC*PDEIPQNLFWKQY*QRP DVLLNNFPATTSTIPSGQPTTIASISPITTTSP KPTSQKH*TSV*PKKPCQ*RQSRWCHAL*WCWK SR*GTRQ*RRNI*RHRSIQRKREEKGKGRKEEVR AGEKGTERERKERTRNKEEI*TNRPYSSHPSCK SLL*CQRRKE*TELQARRAN*NHPHHRQPRRKM VGQNSKGFIWLY*NNCCRD*L*FFETEKRLSWCP FKTY*R*PRSI**CCRAG*Y*QPQSEWKWRDIP STTR**HL*WD*RGRC**WFPCSS*TIGHGR*S LR*CGYL*FPCFISRDESRN*CWKS*DRRKGP*E AKKAGKRRKRLQEKI*I*W*N*SPIFN*SYNFH NF*KVGNQRSTGKTW*ISRSYTNHR*HKSSLQK *RREIWLCPSELPSGQ*WRDL**YC*WLHL*Q*L ALNFGHSAVFIRCQCEVWILIGMLLGIKKINAQ NHLLSFVMKSQLSLQSV*SLNIENNLSA*LLTQ KTTLVRCKNY*IFHFRFGYNYEEVEGTSFRPPVN NPPSKNKNKRKRKIIQEEMTCLKKPKEE**YKK GNLKTFHKKKNYCLYFYLWLYLIFSIQVTCLLK RQCCLTSC*WVKCFQKL*Q**KFCIKFVLIC*LY INVNYLIRMLCI*YAH*SLNCRRV*NKFSFCRF TY*WFNSVLCLKYYYN*SRSE*G*P*NHEEWKN GP*NYLGFYAWHLFIMKTLFKVFVFVSITARFFF LFF*NPFYWKVGQQRE*KLLKF*CLDWALL*QY ILIPKAIQKQNVNHLGFKC*EH*IA*FKK*KML TPKKRRY*VLFNNS*STK*VHHNAGSSTNEPSSD IE*IIYLLSFLICKLQY*TK*VFPTLHYQLP*A FIMLRLLQSHVSEAFVYFLNSLIK**LMHSPHF NIAKAHWSF*SGSTELK*FQITVKELKIFTEMKW GFH*VL*NLITNHQLPLVNI*ILGV*GPKIRCH *SPKIPSNYEIF*CLLLESTSQYMTM*LISFHT R*NYLVQKWFLFIKFGNKYI*YTDRIVFMLKFLA SFSLRTP**AC*TFRVP*DTSSHPSSCLHWGRL ALQSDSNFFSD*ELCKFSTMSVFKSQS*IKRIF QSFGLHIKKQTHIPRKDLHNR*SS*CERYLKY*F *YIKYDFCYSLLYGILSLKMSCK*IIPSM

Sequence NM_001465

CCGCAGTTCTTGAGTTCCACATGCAGAGCAGATGCGACAGCTAGAAGTGAGTAGGGCCCAGACCCTGGCCCAGGAAGATCCACTAAAGGAGGCCATCCTT CCGCCTTCTTCTGCAGGAGTCAGGATGGAAAGGCAGATGTAAAGTCCCTCATGGCGAAATATAACACGGGGGGCAACCCGACAGAGGATGTCTCAGTCAA TAGCCGACCCTTCAGAGTCACAGGGCCAAACTCATCTTCAGGAATACAAGCAAGAAAGAACTTATTCAACAACCAAGGAAATGCCAGCCCTCCTGCAGGA CCCAGCAATGTACCTAAGTTTGGGTCCCCAAAGCCACCTGTGGCAGTCAAACCTTCTTCTGAGGAAAAGCCTGACAAGGAACCCAAGCCCCCGTTTCTAA AGCCCACTGGAGCAGGCCAAAGATTCGGAACACCAGCCAGCTTGACCACCAGAGACCCCGAGGCGAAAGTGGGATTTCTGAAACCTGTAGGCCCCAAGCC CATCAACTTGCCCAAAGAAGATTCCAAACCTACATTTCCCTGGCCTCCTGGAAACAAGCCATCTCTTCACAGTGTAAACCAAGACCATGACTTAAAGCCA CTAGGCCCGAAATCTGGGCCTACTCCTCCAACCTCAGAAAATGAACAGAAGCAAGCGTTTCCCAAATTGACTGGGGTTAAAGGGAAATTTATGTCAGCAT CACAAGATCTTGAACCCAAGCCCCTCTTCCCCAAACCCGCCTTTGGCCAGAAGCCGCCCCTAAGTACCGAGAACTCCCATGAAGACGAAAGCCCCATGAA GAATGTGTCTTCATCAAAAGGGTCCCCAGCTCCCCTGGGAGTCAGGTCCAAAAGCGGCCCTTTAAAACCAGCAAGGGAAGACTCAGAAAATAAAGACCAT GCAGGGGAGATTTCAAGTTTGCCCTTTCCTGGAGTGGTTTTGAAACCTGCTGCGAGCAGGGGAGGCCCAGGTCTCTCCAAAAATGGTGAAGAAAAAAAGG AAGATAGGAAGATAGATGCTGCTAAGAACACCTTCCAGAGCAAAATAAATCAGGAAGAGTTGGCCTCAGGGACTCCTCCTGCCAGGTTCCCTAAGGCCCC TTCTAAGCTGACAGTGGGGGGGCCATGGGGCCAAAGTCAGGAAAAGGAAAAGGGAGACAAGAATTCAGCCACCCCGAAACAGAAGCCATTGCCTCCCTTG TTTACCTTGGGTCCACCTCCACCAAAACCCAACAGACCACCAAATGTTGACCTGACGAAATTCCACAAAACCTCTTCTGGAAACAGTACTAGCAAAGGCC AGACGTCTTACTCAACAACTTCCCTGCCACCACCTCCACCATCCCATCCGGCCAGCCAACCACCATTGCCAGCATCTCACCCATCACAACCACCAGTCCC AAGCCTACCTCCCAGAAACATTAAACCTCCGTTTGACCTAAAAAGCCCTGTCAATGAAGACAATCAAGATGGTGTCACGCACTCTGATGGTGCTGGAAAT CTAGATGAGGAACAAGACAGTGAAGGAGAAACATATGAAGACATAGAAGCATCCAAAGAAAGAGAGAAGAAAAGGGAAAAGGAAGAAAAGAAGAGGTTAG AGCTGGAGAAAAAGGAACAGAAAGAGAAAGAAAAGAAAGAACAAGAAATAAAGAAGAAATTTAAACTAACAGGCCCTATTCAAGTCATCCATCTTGCAAA AGCTTGTTGTGATGTCAAAGGAGGAAAGAATGAACTGAGCTTCAAGCAAGGAGAGCAAATTGAAATCATCCGCATCACAGACAACCCAGAAGGAAAATGG TTGGGCAGAACAGCAAGGGGTTCATATGGCTATATTAAAACAACTGCTGTAGAGATTGACTATGATTCTTTGAAACTGAAAAAAGACTCTCTTGGTGCCC CTTCAAGACCTATTGAAGATGACCAAGAAGTATATGATGATGTTGCAGAGCAGGATGATATTAGCAGCCACAGTCAGAGTGGAAGTGGAGGGATATTCCC TCCACCACCAGATGATGACATTTATGATGGGATTGAAGAGGAAGATGCTGATGATGGCTCCACACTACAGGTTCAAGAGAAGAGTAATACGTGGTCCTGG GGGATTTTGAAGATGTTAAAGGGAAAAGATGACAGAAAGAAAAGTATACGAGAGAAACCTAAAGTCTCTGACTCAGACAATAATGAAGGTTCATCTTTCC CTGCTCCTCCTAAACAATTGGACATGGGAGATGAAGTTTACGATGATGTGGATACCTCTGATTTCCCTGTTTCATCAGCAGAGATGAGTCAAGGAACTAA TGTTGGAAAAGCTAAGACAGAAGAAAAGGACCTTAAGAAGCTAAAAAAGCAGGAAAAAGAAGAAAAAGACTTCAGGAAAAAATTTAAATATGATGGTGAA ATTAGAGTCCTATATTCAACTAAAGTTACAACTTCCATAACTTCTAAAAAGTGGGGAACCAGAGATCTACAGGTAAAACCTGGTGAATCTCTAGAAGTTA TACAAACCACAGATGACACAAAAGTTCTCTGCAGAAATGAAGAAGGGAAATATGGTTATGTCCTTCGGAGTTACCTAGCGGACAATGATGGAGAGATCTA TGATGATATTGCTGATGGCTGCATCTATGACAATGACTAGCACTCAACTTTGGTCATTCTGCTGTGTTCATTAGGTGCCAATGTGAAGTCTGGATTTTAA TTGGCATGTTATTGGGTATCAAGAAAATTAATGCACAAAACCACTTATTATCATTTGTTATGAAATCCCAATTATCTTTACAAAGTGTTTAAAGTTTGAA CATAGAAAATAATCTCTCTGCTTAATTGTTAACTCAGAAGACTACATTAGTGAGATGTAAGAATTATTAAATATTCCATTTCCGCTTTGGCTACAATTAT GAAGAAGTTGAAGGTACTTCTTTTAGACCACCAGTAAATAATCCTCCTTCAAAAAATAAAAATAAAAGAAAAAGGAAAATCATTCAGGAAGAAATGACCT GTCTAAAAAAACCTAAGGAAGAATAATAATATAAGAAAGGAAATTTAAAAACATTCCACAAGAAGAAAAATTATTGTTTATACTTCTACTTATGGTTATA TCTTATATTCTCTATTCAAGTGACCTGTCTTTTAAAAAGGCAGTGCTGTCTTACCTCTTGCTAGTGGGTTAAATGTTTTCAAAAATTATAGCAGTAGTAG AAGTTTTGTATAAAATTTGTCCTTATTTGTTAATTGTATATAAATGTTAATTATTTGATACGAATGTTATGCATTTAGTATGCACATTGAAGTCTAAACT GTAGAAGAGTCTAAAACAAGTTCTCTTTTTGCAGATTCACATACTAATGGTTTAATTCTGTGCTCTGTTTAAAGTACTATTATAACTAGAGTAGATCTGA ATGAGGATAACCCTAAAATCATGAGGAATGGAAGAATGGACCTTGAAACTACCTAGGCTTTTATGCATGGCACCTCTTTATAATGAAGACACTTTTTAAA GTTTTTGTTTTTGTTTCAATTACCGCTAGATTTTTTTTTCTCTTTTTTTAAAATCCATTTTACTGGAAAGTTGGCCAGCAGAGGGAGTAGAAATTATTAA AATTCTAGTGTTTGGATTGGGCCCTTCTCTAACAGTACATACTCATTCCCAAAGCAATCCAAAAACAAAATGTGAACCATTTGGGTTTCAAATGTTAAGA ACACTAAATAGCATGATTTAAAAAATGAAAAATGCTAACACCCAAGAAAAGAAGATATTAAGTGCTTTTTAACAACTCCTAGAGTACAAAATGAGTACAT CATAATGCTGGCTCTTCTACTAATGAACCATCGAGTGATATTGAATAAATTATTTATCTTCTCAGTTTCCTTATCTGTAAATTACAATATTAGACTAAGT AAGTTTTTCCAACTCTTCACTACCAATTACCTTAGGCTTTTATAATGCTCCGCCTACTTCAGTCCCATGTTTCAGAAGCTTTTGTCTATTTTTTAAACTC ATTGATTAAATAATGATTAATGCATTCTCCACATTTTAATATTGCAAAGGCCCATTGGAGTTTCTGAAGTGGCTCCACAGAATTGAAATAATTTCAAATA ACTGTAAAGGAACTGAAAATCTTCACAGAGATGAAGTGGGGTTTCCATTAGGTGCTTTGAAATTTGATAACAAATCATCAACTTCCACTGGTCAATATAT AGATTTTGGGTGTCTGAGGCCCCAAGATTAGATGCCACTAATCTCCAAAGATTCCCTCCAATTATGAAATATTTTAATGTCTACTTTTAGAGAGCACTAG CCAGTATATGACCATGTGATTAATTTCTTTTCACACTAGATAAAATTACCTGGTTCAAAAGTGGTTTTTGTTTATTAAATTTGGTAATAAATATATATAA TACACAGACAGGATAGTTTTTATGCTGAAGTTTTTGGCCAGCTTTAGTTTGAGGACTCCTTGATAAGCTTGCTAAACTTTCAGAGTGCCCTGAGACACTT CCAGCCATCCCTCCTCCTGCCTTCATTGGGGCAGACTTGCATTGCAGTCTGACAGTAATTTTTTTTCTGATTGAGAATTATGTAAATTCAGTACAATGTC AGTTTTTAAAAGTCAAAGTTAGATCAAGAGAATATTTCAGAGTTTTGGTTTACACATCAAGAAACAGACACACATACCTAGGAAAGATTTACACAATAGA TAATCATCTTAATGTGAAAGATATTTGAAGTATTAATTTTAATATATTAAATATGATTTCTGTTATAGTCTTCTGTATGGAATTTTGTCACTTAAGATGA GCTGCAAATAAATAATACCTTCAATGGA

refseq_FYB.F1 (forward primer)
refseq_FYB.R1 (reverse primer)
Single exon skipping

Frame 0

PQFLSSTCRADATARSE*GPDPGPGRSTKGGHP SAFFCRSQDGKADVKSLMAKYNTGGNPTEDVSVN SRPFRVTGPNSSSGIQARKNLFNNQGNASPPAG PSNVPKFGSPKPPVAVKPSSEEKPDKEPKPPFL KPTGAGQRFGTPASLTTRDPEAKVGFLKPVGPKP INLPKEDSKPTFPWPPGNKPSLHSVNQDHDLKP LGPKSGPTPPTSENEQKQAFPKLTGVKGKFMSA SQDLEPKPLFPKPAFGQKPPLSTENSHEDESPMK NVSSSKGSPAPLGVRSKSGPLKPAREDSENKDH AGEISSLPFPGVVLKPAASRGGPGLSKNGEEKK EDRKIDAAKNTFQSKINQEELASGTPPARFPKAP SKLTVGGPWGQSQEKEKGDKNSATPKQKPLPPL FTLGPPPPKPNRPPNVDLTKFHKTSSGNSTSKG QTSYSTTSLPPPPPSHPASQPPLPASHPSQPPVP SLPPRNIKPPFDLKSPVNEDNQDGVTHSDGAGN LDEEQDSEGETYEDIEASKEREKKREKEEKKRL ELEKKEQKEKEKKEQEIKKKFKLTGPIQVIHLAK ACCDVKGGKNELSFKQGEQIEIIRITDNPEGKW LGRTARGSYGYIKTTAVEIDYDSLKLKKDSLGA PSRPIEDDQEVYDDVAEQDDISSHSQSGSGGIFP PPPDDDIYDGIEEEDADDGSTLQVQEKSNTWSW GILKMLKGKDDRKKSIREKPKVSDSDNNEGSSF PAPPKQLDMGDEVYDDVDTSDFPVSSAEMSQGTN VGKAKTEEKDLKKLKKQEKEEKDFRKKFKYDGE IRVLYSTKVTTSITSKKWGTRDLQVKPGESLEV IQTTDDTKVLCRNEEGKYGYVLRSYLADNDGEIY DDIADGCIYDND*HSTLVILLCSLGANVKSGF* LACYWVSRKLMHKTTYYHLL*NPNYLYKVFKV* T*KIISLLNC*LRRLH**DVRIIKYSISALATIM KKLKVLLLDHQ*IILLQKIKIKEKGKSFRKK*P V*KNLRKNNNIRKEI*KHSTRRKIIVYTSTYGY ILYSLFK*PVF*KGSAVLPLASGLNVFKNYSSSR SFV*NLSLFVNCI*MLII*YECYAFSMHIEV*T VEESKTSSLFADSHTNGLILCSV*STIITRVDL NEDNPKIMRNGRMDLETT*AFMHGTSL**RHFLK FLFLFQLPLDFFFSFFKIHFTGKLASRGSRNY* NSSVWIGPFSNSTYSFPKQSKNKM*TIWVSNVK NTK*HDLKNEKC*HPRKEDIKCFLTTPRVQNEYI IMLALLLMNHRVILNKLFIFSVSLSVNYNIRLS KFFQLFTTNYLRLL*CSAYFSPMFQKLLSIF*T H*LNND*CILHILILQRPIGVSEVAPQN*NNFK* L*RN*KSSQR*SGVSIRCFEI**QIINFHWSIY RFWVSEAPRLDATNLQRFPPIMKYFNVYF*RAL ASI*PCD*FLFTLDKITWFKSGFCLLNLVINIYN TQTG*FLC*SFWPALV*GLLDKLAKLSECPETL PAIPPPAFIGADLHCSLTVIFFLIENYVNSVQC QFLKVKVRSREYFRVLVYTSRNRHTYLGKIYTID NHLNVKDI*SINFNILNMISVIVFCMEFCHLR* AANK*YLQW

Frame 1

RSS*VPHAEQMRQLEVSRAQTLAQEDPLKEAIL PPSSAGVRMERQM*SPSWRNITRGATRQRMSQS IADPSESQGQTHLQEYKQERTYSTTKEMPALLQD PAMYLSLGPQSHLWQSNLLLRKSLTRNPSPRF* SPLEQAKDSEHQPA*PPETPRRKWDF*NL*APS PSTCPKKIPNLHFPGLLETSHLFTV*TKTMT*SH *ARNLGLLLQPQKMNRSKRFPN*LGLKGNLCQH HKILNPSPSSPNPPLARSRP*VPRTPMKTKAP* RMCLHQKGPQLPWESGPKAAL*NQQGKTQKIKTM QGRFQVCPFLEWF*NLLRAGEAQVSPKMVKKKR KIGR*MLLRTPSRAK*IRKSWPQGLLLPGSLRP LLS*QWGGHGAKVRKRKRETRIQPPRNRSHCLPC LPWVHLHQNPTDHQMLT*RNSTKPLLETVLAKA RRLTQQLPCHHLHHPIRPANHHCQHLTHHNHQS QAYLPETLNLRLT*KALSMKTIKMVSRTLMVLEI *MRNKTVKEKHMKT*KHPKKERRKGKRKKRRG* SWRKRNRKRKKRKNKK*RRNLN*QALFKSSILQ KLVVMSKEERMN*ASSKESKLKSSASQTTQKENG WAEQQGVHMAILKQLL*RLTMIL*N*KKTLLVP LQDLLKMTKKYMMMLQSRMILAATVRVEVEGYS LHHQMMTFMMGLKRKMLMMAPHYRFKRRVIRGPG GF*RC*REKMTERKVYERNLKSLTQTIMKVHLS LLLLNNWTWEMKFTMMWIPLISLFHQQR*VKEL MLEKLRQKKRTLRS*KSRKKKKKTSGKNLNMMVK LESYIQLKLQLP*LLKSGEPEIYR*NLVNL*KL YKPQMTQKFSAEMKKGNMVMSFGVT*RTMMERS MMILLMAASMTMTSTQLWSFCCVH*VPM*SLDFN WHVIGYQEN*CTKPLIIICYEIPIIFTKCLKFE HRK*SLCLIVNSEDYISEM*ELLNIPFPLWLQL *RS*RYFF*TTSK*SSFKK*K*KKKENHSGRNDL SKKT*GRIII*ERKFKNIPQEEKLLFILLLMVI SYILYSSDLSFKKAVLSYLLLVG*MFSKIIAVV EVLYKICPYLLIVYKC*LFDTNVMHLVCTLKSKL *KSLKQVLFLQIHILMV*FCALFKVLL*LE*I* MRITLKS*GMEEWTLKLPRLLCMAPLYNEDTF* SFCFCFNYR*IFFSLFLKSILLESWPAEGVEIIK ILVFGLGPSLTVHTHSQSNPKTKCEPFGFQMLR TLNSMI*KMKNANTQEKKILSAF*QLLEYKMST S*CWLFY**TIE*Y*INYLSSQFPYL*ITILD*V SFSNSSLPITLGFYNAPPTSVPCFRSFCLFFKL ID*IMINAFSTF*YCKGPLEFLKWLHRIEIISN NCKGTENLHRDEVGFPLGALKFDNKSSTSTGQYI DFGCLRPQD*MPLISKDSLQL*NILMSTFREH* PVYDHVINFFSH*IKLPGSKVVFVY*IW**IYI IHRQDSFYAEVFGQL*FEDSLISLLNFQSALRHF QPSLLLPSLGQTCIAV*Q*FFF*LRIM*IQYNV SF*KSKLDQENISEFWFTHQETDTHT*ERFTQ* IIILM*KIFEVLILIY*I*FLL*SSVWNFVT*DE LQINNTFNG

Frame 2

AVLEFHMQSRCDS*K*VGPRPWPRKIH*RRPSF RLLLQESGWKGRCKVPHGEI*HGGQPDRGCLSQ *PTLQSHRAKLIFRNTSKKELIQQPRKCQPSCR TQQCT*VWVPKATCGSQTFF*GKA*QGTQAPVSK AHWSRPKIRNTSQLDHQRPRGESGISETCRPQA HQLAQRRFQTYISLASWKQAISSQCKPRP*LKA TRPEIWAYSSNLRK*TEASVSQIDWG*REIYVSI TRS*TQAPLPQTRLWPEAAPKYRELP*RRKPHE ECVFIKRVPSSPGSQVQKRPFKTSKGRLRK*RP CRGDFKFALSWSGFETCCEQGRPRSLQKW*RKKG R*EDRCC*EHLPEQNKSGRVGLRDSSCQVP*GP F*ADSGGAMGPKSGKGKGRQEFSHPETEAIASL VYLGSTSTKTQQTTKC*PDEIPQNLFWKQY*QRP DVLLNNFPATTSTIPSGQPTTIASISPITTTSP KPTSQKH*TSV*PKKPCQ*RQSRWCHAL*WCWK SR*GTRQ*RRNI*RHRSIQRKREEKGKGRKEEVR AGEKGTERERKERTRNKEEI*TNRPYSSHPSCK SLL*CQRRKE*TELQARRAN*NHPHHRQPRRKM VGQNSKGFIWLY*NNCCRD*L*FFETEKRLSWCP FKTY*R*PRSI**CCRAG*Y*QPQSEWKWRDIP STTR**HL*WD*RGRC**WLHTTGSREE*YVVL GDFEDVKGKR*QKEKYTRET*SL*LRQ**RFIFP CSS*TIGHGR*SLR*CGYL*FPCFISRDESRN* CWKS*DRRKGP*EAKKAGKRRKRLQEKI*I*W* N*SPIFN*SYNFHNF*KVGNQRSTGKTW*ISRSY TNHR*HKSSLQK*RREIWLCPSELPSGQ*WRDL **YC*WLHL*Q*LALNFGHSAVFIRCQCEVWIL IGMLLGIKKINAQNHLLSFVMKSQLSLQSV*SLN IENNLSA*LLTQKTTLVRCKNY*IFHFRFGYNY EEVEGTSFRPPVNNPPSKNKNKRKRKIIQEEMT CLKKPKEE**YKKGNLKTFHKKKNYCLYFYLWLY LIFSIQVTCLLKRQCCLTSC*WVKCFQKL*Q** KFCIKFVLIC*LYINVNYLIRMLCI*YAH*SLN CRRV*NKFSFCRFTY*WFNSVLCLKYYYN*SRSE *G*P*NHEEWKNGP*NYLGFYAWHLFIMKTLFK VFVFVSITARFFFLFF*NPFYWKVGQQRE*KLL KF*CLDWALL*QYILIPKAIQKQNVNHLGFKC*E H*IA*FKK*KMLTPKKRRY*VLFNNS*STK*VH HNAGSSTNEPSSDIE*IIYLLSFLICKLQY*TK *VFPTLHYQLP*AFIMLRLLQSHVSEAFVYFLNS LIK**LMHSPHFNIAKAHWSF*SGSTELK*FQI TVKELKIFTEMKWGFH*VL*NLITNHQLPLVNI *ILGV*GPKIRCH*SPKIPSNYEIF*CLLLESTS QYMTM*LISFHTR*NYLVQKWFLFIKFGNKYI* YTDRIVFMLKFLASFSLRTP**AC*TFRVP*DT SSHPSSCLHWGRLALQSDSNFFSD*ELCKFSTMS VFKSQS*IKRIFQSFGLHIKKQTHIPRKDLHNR *SS*CERYLKY*F*YIKYDFCYSLLYGILSLKM SCK*IIPSM