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Sequences

Sequence NM_013986

AGAGGGAAAGCGAGAGGGAGACGGACGTTGAGAGAACGAGGAGGAAGGAGAGAAAATGGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAG
CAGGGCTACAGTGCTTACACCGCCCAGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCCCACTG
ATGTCAGCTATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTATGCAACTTCTTATGGACAGCCTCCCACTGGTTATACTACTCCAACTGC
CCCCCAGGCATACAGCCAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGATACCACCACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAG
TCTGCATATGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACAAGACCGCAGGATGGAAACAAGCCCACTGAGACTA
GTCAACCTCAATCTAGCACAGGGGGTTACAACCAGCCCAGCCTAGGATATGGACAGAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTACCCCATGCA
GCCAGTCACTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCGACTAGTTATGATCAGAGCAGTTACTCTCAGCAGAACACCTATGGG
CAACCGAGCAGCTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGCAGCAGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCC
AAGCTCCAAGTCAATATAGCCAACAGAGCAGCAGCTACGGGCAGCAGAGACCCATGGATGAAGGACCAGATCTTGATCTAGGCCCACCTGTAGATCCAGA
TGAAGACTCTGACAACAGTGCAATTTATGTACAAGGATTAAATGACAGTGTGACTCTAGATGATCTGGCAGACTTCTTTAAGCAGTGTGGGGTTGTTAAG
ATGAACAAGAGAACTGGGCAACCCATGATCCACATCTACCTGGACAAGGAAACAGGAAAGCCCAAAGGCGATGCCACAGTGTCCTATGAAGACCCACCCA
CTGCCAAGGCTGCCGTGGAATGGTTTGATGGGAAAGATTTTCAAGGGAGCAAACTTAAAGTCTCCCTTGCTCGGAAGAAGCCTCCAATGAACAGTATGCG
GGGTGGTCTGCCACCCCGTGAGGGCAGAGGCATGCCACCACCACTCCGTGGAGGTCCAGGAGGCCCAGGAGGTCCTGGGGGACCCATGGGTCGCATGGGA
GGCCGTGGAGGAGATAGAGGAGGCTTCCCTCCAAGAGGACCCCGGGGTTCCCGAGGGAACCCCTCTGGAGGAGGAAACGTCCAGCACCGAGCTGGAGACT
GGCAGTGTCCCAATCCGGGTTGTGGAAACCAGAACTTCGCCTGGAGAACAGAGTGCAACCAGTGTAAGGCCCCAAAGCCTGAAGGCTTCCTCCCGCCACC
CTTTCCGCCCCCGGGTGGTGATCGTGGCAGAGGTGGCCCTGGTGGCATGCGGGGAGGAAGAGGTGGCCTCATGGATCGTGGTGGTCCCGGTGGAATGTTC
AGAGGTGGCCGTGGTGGAGACAGAGGTGGCTTCCGTGGTGGCCGGGGCATGGACCGAGGTGGCTTTGGTGGAGGAAGACGAGGTGGCCCTGGGGGGCCCC
CTGGACCTTTGATGGAACAGATGGGAGGAAGAAGAGGAGGACGTGGAGGACCTGGAAAAATGGATAAAGGCGAGCACCGTCAGGAGCGCAGAGATCGGCC
CTACTAGATGCAGAGACCCCGCAGAGCTGCATTGACTACCAGATTTATTTTTTAAACCAGAAAATGTTTTAAATTTATAATTCCATATTTATAATGTTGG
CCACAACATTATGATTATTCCTTGTCTGTACTTTAGTATTTTTCACCATTTGTGAAGAAACATTAAAACAAGTTAAATGGTAGTGTGCGGAGTTTTTTTT
TCTTCCTTCTTTTAAAAATGGTTGTTTAAGACTTTAACAATGGGAACCCCTTGTGAGCATGCTCAGTATCATTGTGGAGAACCAAGAGGGCCTCTTAACT
GTAACAATGTTCATGGTTGTGATGTTTTTTTTTTTTTTTTAAATAAAATTCCAAATGTTTA
  • refseq_EWSR1.F1 (forward primer)
  • refseq_EWSR1.R1 (reverse primer)
  • Multiple exon skipping

Frame 0

RGKARGRRTLRERGGRRENGVHGLQYL*PSCSA
AGLQCLHRPAHSRICTDHPGIWATKLWNLWTAH*
CQLYPGSDHCNLWADRLCNFLWTASHWLYYSNC
PPGIQPACPGVWHWCL*YHHCYSHHHPGLLCSS
VCIWHSACLSSLWAAASSHCTYKTAGWKQAH*D*
STSI*HRGLQPAQPRIWTE*LQLSPGTWELPHA
ASHCTSILPSYQLFLYTAD*L*SEQLLSAEHLW
ATEQLWTAE*LWSTKQLWAAASH*LPTPNWILQP
SSKSI*PTEQQLRAAETHG*RTRS*SRPTCRSR
*RL*QQCNLCTRIK*QCDSR*SGRLL*AVWGC*
DEQENWATHDPHLPGQGNRKAQRRCHSVL*RPTH
CQGCRGMV*WERFSREQT*SLPCSEEASNEQYA
GWSATP*GQRHATTTPWRSRRPRRSWGTHGSHG
RPWRR*RRLPSKRTPGFPREPLWRRKRPAPSWRL
AVSQSGLWKPELRLENRVQPV*GPKA*RLPPAT
LSAPGW*SWQRWPWWHAGRKRWPHGSWWSRWNV
QRWPWWRQRWLPWWPGHGPRWLWWRKTRWPWGAP
WTFDGTDGRKKRRTWRTWKNG*RRAPSGAQRSA
LLDAETPQSCIDYQIYFLNQKMF*IYNSIFIML
ATTL*LFLVCTLVFFTICEETLKQVKW*CAEFFF
LPSFKNGCLRL*QWEPLVSMLSIIVENQEGLLT
VTMFMVVMFFFFF*IKFQMF

Frame 1

EGKREGDGR*ENEEEGEKMASTDYSTYSQAAAQ
QGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPT
DVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTA
PQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQ
SAYGTQPAYPAYGQQPAATAPTRPQDGNKPTET
SQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQ
PVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYG
QPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYS
QAPSQYSQQSSSYGQQRPMDEGPDLDLGPPVDPD
EDSDNSAIYVQGLNDSVTLDDLADFFKQCGVVK
MNKRTGQPMIHIYLDKETGKPKGDATVSYEDPP
TAKAAVEWFDGKDFQGSKLKVSLARKKPPMNSMR
GGLPPREGRGMPPPLRGGPGGPGGPGGPMGRMG
GRGGDRGGFPPRGPRGSRGNPSGGGNVQHRAGD
WQCPNPGCGNQNFAWRTECNQCKAPKPEGFLPPP
FPPPGGDRGRGGPGGMRGGRGGLMDRGGPGGMF
RGGRGGDRGGFRGGRGMDRGGFGGGRRGGPGGP
PGPLMEQMGGRRGGRGGPGKMDKGEHRQERRDRP
Y*MQRPRRAALTTRFIF*TRKCFKFIIPYL*CW
PQHYDYSLSVL*YFSPFVKKH*NKLNGSVRSFF
FFLLLKMVV*DFNNGNPL*ACSVSLWRTKRAS*L
*QCSWL*CFFFFFK*NSKCL

Frame 2

RESERETDVERTRRKERKWRPRITVPIAKLQRS
RATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPL
MSAIPRLRPLQPMGRPPMQLLMDSLPLVILLQL
PPRHTASLSRGMALVLMIPPLLQSPPPRPPMQLS
LHMALSLLIQPMGSSQQPLHLQDRRMETSPLRL
VNLNLAQGVTTSPA*DMDRVTTVIPRYLGATPC
SQSLHLHPTLLPAIPLHSRLVMIRAVTLSRTPMG
NRAAMDSRVAMVNKAAMGSSLPLVTHPKLDPTA
KLQVNIANRAAATGSRDPWMKDQILI*AHL*IQ
MKTLTTVQFMYKD*MTV*L*MIWQTSLSSVGLLR
*TRELGNP*STSTWTRKQESPKAMPQCPMKTHP
LPRLPWNGLMGKIFKGANLKSPLLGRSLQ*TVC
GVVCHPVRAEACHHHSVEVQEAQEVLGDPWVAWE
AVEEIEEASLQEDPGVPEGTPLEEETSSTELET
GSVPIRVVETRTSPGEQSATSVRPQSLKASSRH
PFRPRVVIVAEVALVACGEEEVASWIVVVPVECS
EVAVVETEVASVVAGAWTEVALVEEDEVALGGP
LDL*WNRWEEEEEDVEDLEKWIKASTVRSAEIG
PTRCRDPAELH*LPDLFFKPENVLNL*FHIYNVG
HNIMIIPCLYFSIFHHL*RNIKTS*MVVCGVFF
SSFF*KWLFKTLTMGTPCEHAQYHCGEPRGPLN
CNNVHGCDVFFFFLNKIPNV

Sequence NM_005243

AGAGGGAAAGCGAGAGGGAGACGGACGTTGAGAGAACGAGGAGGAAGGAGAGAAAATGGCGTCCACGGATTACAGTACCTATAGCCAAGCTGCAGCGCAG
CAGGGCTACAGTGCTTACACCGCCCAGCCCACTCAAGGATATGCACAGACCACCCAGGCATATGGGCAACAAAGCTATGGAACCTATGGACAGCCCACTG
ATGTCAGCTATACCCAGGCTCAGACCACTGCAACCTATGGGCAGACCGCCTATGCAACTTCTTATGGACAGCCTCCCACTGGTTATACTACTCCAACTGC
CCCCCAGGCATACAGCCAGCCTGTCCAGGGGTATGGCACTGGTGCTTATGATACCACCACTGCTACAGTCACCACCACCCAGGCCTCCTATGCAGCTCAG
TCTGCATATGGCACTCAGCCTGCTTATCCAGCCTATGGGCAGCAGCCAGCAGCCACTGCACCTACAAGACCGCAGGATGGAAACAAGCCCACTGAGACTA
GTCAACCTCAATCTAGCACAGGGGGTTACAACCAGCCCAGCCTAGGATATGGACAGAGTAACTACAGTTATCCCCAGGTACCTGGGAGCTACCCCATGCA
GCCAGTCACTGCACCTCCATCCTACCCTCCTACCAGCTATTCCTCTACACAGCCGACTAGTTATGATCAGAGCAGTTACTCTCAGCAGAACACCTATGGG
CAACCGAGCAGCTATGGACAGCAGAGTAGCTATGGTCAACAAAGCAGCTATGGGCAGCAGCCTCCCACTAGTTACCCACCCCAAACTGGATCCTACAGCC
AAGCTCCAAGTCAATATAGCCAACAGAGCAGCAGCTACGGGCAGCAGAGTTCATTCCGACAGGACCACCCCAGTAGCATGGGTGTTTATGGGCAGGAGTC
TGGAGGATTTTCCGGACCAGGAGAGAACCGGAGCATGAGTGGCCCTGATAACCGGGGCAGGGGAAGAGGGGGATTTGATCGTGGAGGCATGAGCAGAGGT
GGGCGGGGAGGAGGACGCGGTGGAATGGGCAGCGCTGGAGAGCGAGGTGGCTTCAATAAGCCTGGTGGACCCATGGATGAAGGACCAGATCTTGATCTAG
GCCCACCTGTAGATCCAGATGAAGACTCTGACAACAGTGCAATTTATGTACAAGGATTAAATGACAGTGTGACTCTAGATGATCTGGCAGACTTCTTTAA
GCAGTGTGGGGTTGTTAAGATGAACAAGAGAACTGGGCAACCCATGATCCACATCTACCTGGACAAGGAAACAGGAAAGCCCAAAGGCGATGCCACAGTG
TCCTATGAAGACCCACCCACTGCCAAGGCTGCCGTGGAATGGTTTGATGGGAAAGATTTTCAAGGGAGCAAACTTAAAGTCTCCCTTGCTCGGAAGAAGC
CTCCAATGAACAGTATGCGGGGTGGTCTGCCACCCCGTGAGGGCAGAGGCATGCCACCACCACTCCGTGGAGGTCCAGGAGGCCCAGGAGGTCCTGGGGG
ACCCATGGGTCGCATGGGAGGCCGTGGAGGAGATAGAGGAGGCTTCCCTCCAAGAGGACCCCGGGGTTCCCGAGGGAACCCCTCTGGAGGAGGAAACGTC
CAGCACCGAGCTGGAGACTGGCAGTGTCCCAATCCGGGTTGTGGAAACCAGAACTTCGCCTGGAGAACAGAGTGCAACCAGTGTAAGGCCCCAAAGCCTG
AAGGCTTCCTCCCGCCACCCTTTCCGCCCCCGGGTGGTGATCGTGGCAGAGGTGGCCCTGGTGGCATGCGGGGAGGAAGAGGTGGCCTCATGGATCGTGG
TGGTCCCGGTGGAATGTTCAGAGGTGGCCGTGGTGGAGACAGAGGTGGCTTCCGTGGTGGCCGGGGCATGGACCGAGGTGGCTTTGGTGGAGGAAGACGA
GGTGGCCCTGGGGGGCCCCCTGGACCTTTGATGGAACAGATGGGAGGAAGAAGAGGAGGACGTGGAGGACCTGGAAAAATGGATAAAGGCGAGCACCGTC
AGGAGCGCAGAGATCGGCCCTACTAGATGCAGAGACCCCGCAGAGCTGCATTGACTACCAGATTTATTTTTTAAACCAGAAAATGTTTTAAATTTATAAT
TCCATATTTATAATGTTGGCCACAACATTATGATTATTCCTTGTCTGTACTTTAGTATTTTTCACCATTTGTGAAGAAACATTAAAACAAGTTAAATGGT
AGTGTGCGGAGTTTTTTTTTCTTCCTTCTTTTAAAAATGGTTGTTTAAGACTTTAACAATGGGAACCCCTTGTGAGCATGCTCAGTATCATTGTGGAGAA
CCAAGAGGGCCTCTTAACTGTAACAATGTTCATGGTTGTGATGTTTTTTTTTTTTTTTTAAATAAAATTCCAAATGTTTA
  • refseq_EWSR1.F1 (forward primer)
  • refseq_EWSR1.R1 (reverse primer)
  • Multiple exon skipping

Frame 0

RGKARGRRTLRERGGRRENGVHGLQYL*PSCSA
AGLQCLHRPAHSRICTDHPGIWATKLWNLWTAH*
CQLYPGSDHCNLWADRLCNFLWTASHWLYYSNC
PPGIQPACPGVWHWCL*YHHCYSHHHPGLLCSS
VCIWHSACLSSLWAAASSHCTYKTAGWKQAH*D*
STSI*HRGLQPAQPRIWTE*LQLSPGTWELPHA
ASHCTSILPSYQLFLYTAD*L*SEQLLSAEHLW
ATEQLWTAE*LWSTKQLWAAASH*LPTPNWILQP
SSKSI*PTEQQLRAAEFIPTGPPQ*HGCLWAGV
WRIFRTRREPEHEWP**PGQGKRGI*SWRHEQR
WAGRRTRWNGQRWRARWLQ*AWWTHG*RTRS*SR
PTCRSR*RL*QQCNLCTRIK*QCDSR*SGRLL*
AVWGC*DEQENWATHDPHLPGQGNRKAQRRCHS
VL*RPTHCQGCRGMV*WERFSREQT*SLPCSEEA
SNEQYAGWSATP*GQRHATTTPWRSRRPRRSWG
THGSHGRPWRR*RRLPSKRTPGFPREPLWRRKR
PAPSWRLAVSQSGLWKPELRLENRVQPV*GPKA*
RLPPATLSAPGW*SWQRWPWWHAGRKRWPHGSW
WSRWNVQRWPWWRQRWLPWWPGHGPRWLWWRKT
RWPWGAPWTFDGTDGRKKRRTWRTWKNG*RRAPS
GAQRSALLDAETPQSCIDYQIYFLNQKMF*IYN
SIFIMLATTL*LFLVCTLVFFTICEETLKQVKW
*CAEFFFLPSFKNGCLRL*QWEPLVSMLSIIVEN
QEGLLTVTMFMVVMFFFFF*IKFQMF

Frame 1

EGKREGDGR*ENEEEGEKMASTDYSTYSQAAAQ
QGYSAYTAQPTQGYAQTTQAYGQQSYGTYGQPT
DVSYTQAQTTATYGQTAYATSYGQPPTGYTTPTA
PQAYSQPVQGYGTGAYDTTTATVTTTQASYAAQ
SAYGTQPAYPAYGQQPAATAPTRPQDGNKPTET
SQPQSSTGGYNQPSLGYGQSNYSYPQVPGSYPMQ
PVTAPPSYPPTSYSSTQPTSYDQSSYSQQNTYG
QPSSYGQQSSYGQQSSYGQQPPTSYPPQTGSYS
QAPSQYSQQSSSYGQQSSFRQDHPSSMGVYGQES
GGFSGPGENRSMSGPDNRGRGRGGFDRGGMSRG
GRGGGRGGMGSAGERGGFNKPGGPMDEGPDLDL
GPPVDPDEDSDNSAIYVQGLNDSVTLDDLADFFK
QCGVVKMNKRTGQPMIHIYLDKETGKPKGDATV
SYEDPPTAKAAVEWFDGKDFQGSKLKVSLARKK
PPMNSMRGGLPPREGRGMPPPLRGGPGGPGGPGG
PMGRMGGRGGDRGGFPPRGPRGSRGNPSGGGNV
QHRAGDWQCPNPGCGNQNFAWRTECNQCKAPKP
EGFLPPPFPPPGGDRGRGGPGGMRGGRGGLMDRG
GPGGMFRGGRGGDRGGFRGGRGMDRGGFGGGRR
GGPGGPPGPLMEQMGGRRGGRGGPGKMDKGEHR
QERRDRPY*MQRPRRAALTTRFIF*TRKCFKFII
PYL*CWPQHYDYSLSVL*YFSPFVKKH*NKLNG
SVRSFFFFLLLKMVV*DFNNGNPL*ACSVSLWR
TKRAS*L*QCSWL*CFFFFFK*NSKCL

Frame 2

RESERETDVERTRRKERKWRPRITVPIAKLQRS
RATVLTPPSPLKDMHRPPRHMGNKAMEPMDSPL
MSAIPRLRPLQPMGRPPMQLLMDSLPLVILLQL
PPRHTASLSRGMALVLMIPPLLQSPPPRPPMQLS
LHMALSLLIQPMGSSQQPLHLQDRRMETSPLRL
VNLNLAQGVTTSPA*DMDRVTTVIPRYLGATPC
SQSLHLHPTLLPAIPLHSRLVMIRAVTLSRTPMG
NRAAMDSRVAMVNKAAMGSSLPLVTHPKLDPTA
KLQVNIANRAAATGSRVHSDRTTPVAWVFMGRS
LEDFPDQERTGA*VALITGAGEEGDLIVEA*AEV
GGEEDAVEWAALESEVASISLVDPWMKDQILI*
AHL*IQMKTLTTVQFMYKD*MTV*L*MIWQTSL
SSVGLLR*TRELGNP*STSTWTRKQESPKAMPQC
PMKTHPLPRLPWNGLMGKIFKGANLKSPLLGRS
LQ*TVCGVVCHPVRAEACHHHSVEVQEAQEVLG
DPWVAWEAVEEIEEASLQEDPGVPEGTPLEEETS
STELETGSVPIRVVETRTSPGEQSATSVRPQSL
KASSRHPFRPRVVIVAEVALVACGEEEVASWIV
VVPVECSEVAVVETEVASVVAGAWTEVALVEEDE
VALGGPLDL*WNRWEEEEEDVEDLEKWIKASTV
RSAEIGPTRCRDPAELH*LPDLFFKPENVLNL*
FHIYNVGHNIMIIPCLYFSIFHHL*RNIKTS*MV
VCGVFFSSFF*KWLFKTLTMGTPCEHAQYHCGE
PRGPLNCNNVHGCDVFFFFLNKIPNV

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