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Sequences

Sequence NM_178156

CGTTTAGTACAGAAATCTCATGGGAGAGAGCATCCATGCATTTACAAATTGTTATTGAATTATTTTATTGAATGATGACACCCAAACTGAGCTAGAACAT
AATTCTGGCTCTGCTAGTACATCTTCTGTGTGATCTTGGACAAGTCACTCTACTTTCCTTTCAATTTTCTTTTCTCACAGGGAGATAATCATAAAAACGA
CTGTAAAGTACAGCACTTCATAGAGTGCTTTTTGTTTAAAGAGCTGACAATAAATACGAGTCTCAAGGTCTAGGAAAGCCTCCCTCACAACCTGAGCTGC
TTGAGGACAAGGGATTTTCTTTTGAATCAGCAGTACCTTATTTGTGTATCTGTGATAGAGTTCCTGGTACATAAGAAGGTCTCAATAAATATGTGAATTT
ATGAATATTAGGCAGATTGCAACCTTGACAGGCCACTGCCTCTTAAATCTCCTTTCTGTGATCTTTTAATATTTAACATCTAAAAGGCCGCCGCTACTTG
CTTTGGGATAAGTATCCCCGGTATGTACTTTAAAATGCCCAAGCCTAGAGAAATGATTCTTGTCTTAAGGGCACCATTTCGCTCTCCCACCGTAAAGCGC
CCCAGGCTTGGGATCTGGGTCCCAAGGCTACAGGGAAGAGTTTGGAACGGGAAGCTCATCTTCCGGCCCTCTGATTGGCCGGCTCGCACTCCACTCACGC
GGCGCGCAGCTCTGATTGGCCTCGGCGGCACCCCTCGTCCCGCGACTACTTTGTGTGCTGGGGCGGCGCGCTCCGGTCCTCCCGCTCAGCTGGCGGTCTG
GGCTGCTCTGGGGCAGCCCTTCGGTCCACTGCTCTGCATCGCGGGCGCCGGGAATTTTCCGAGTCCGAGCGGCATGTAGAGCGCATGAAGTACAGGACAA
TAAAGCTTCCTACACATATCACCAGGAGGATCTCTTTGAAAGATTCACTGCAGGACTACCAGAGAGAATAATTTGTCTGAAGCATCATGTGTTGAAACAA
CAGAAGTCTATTCACCTGTGCACTAACTAGAAACAGAGTTACAATGTTTTCAATTCTTTGAGCTCCAGGACTCCAGGGAAGTGAGTTGAAAATCTGAAAA
TGCGGCCATGGACTGGTTCCTGGCGTTGGATTATGCTCATTCTTTTTGCCTGGGGGACCTTGCTGTTTTATATAGGTGGTCACTTGGTACGAGATAATGA
CCATCCTGATCACTCTAGCCGAGAACTGTCCAAGATTCTGGCAAAGCTTGAACGCTTAAAACAACAGAATGAAGACTTGAGGCGAATGGCCGAATCTCTC
CGGATACCAGAAGGCCCTATTGATCAGGGGCCAGCTATAGGAAGAGTACGCGTTTTAGAAGAGCAGCTTGTTAAGGCCAAAGAACAGATTGAAAATTACA
AGAAACAGACCAGAAATGGTCTGGGGAAGGATCATGAAATCCTGAGGAGGAGGATTGAAAATGGAGCTAAAGAGCTCTGGTTTTTCCTACAGAGTGAATT
GAAGAAATTAAAGAACTTAGAAGGAAATGAACTCCAAAGACATGCAGATGAATTTCTTTTGGATTTAGGACATCATGAAAGGTCTATAATGACGGATCTA
TACTACCTCAGTCAGACAGATGGAGCAGGTGATTGGCGGGAAAAAGAGGCCAAAGATCTGACAGAACTGGTTCAGCGGAGAATAACATATCTTCAGAATC
CCAAGGACTGCAGCAAAGCCAAAAAGCTGGTGTGTAATATCAACAAAGGCTGTGGCTATGGCTGTCAGCTCCATCATGTGGTCTACTGCTTCATGATTGC
ATATGGCACCCAGCGAACACTCATCTTGGAATCTCAGAATTGGCGCTATGCTACTGGTGGATGGGAGACTGTATTTAGGCCTGTAAGTGAGACATGCACA
GACAGATCTGGCATCTCCACTGGACACTGGTCAGGTGAAGTGAAGGACAAAAATGTTCAAGTGGTCGAGCTTCCCATTGTAGACAGTCTTCATCCCCGTC
CTCCATATTTACCCTTGGCTGTACCAGAAGACCTCGCAGATCGACTTGTACGAGTGCATGGTGACCCTGCAGTGTGGTGGGTGTCTCAGTTTGTCAAATA
CTTGATCCGCCCACAGCCTTGGCTAGAAAAAGAAATAGAAGAAGCCACCAAGAAGCTTGGCTTCAAACATCCAGTTATTGGAGTCCATGTCAGACGCACA
GACAAAGTGGGAACAGAAGCTGCCTTCCATCCCATTGAAGAGTACATGGTGCATGTTGAAGAACATTTTCAGCTTCTTGCACGCAGAATGCAAGTGGACA
AAAAAAGAGTGTATTTGGCCACAGATGACCCTTCTTTATTAAAGGAGGCAAAAACAAAGTACCCCAATTATGAATTTATTAGTGATAACTCTATTTCCTG
GTCAGCTGGACTGCACAATCGATACACAGAAAATTCACTTCGTGGAGTGATCCTGGATATACATTTTCTCTCTCAGGCAGACTTCCTAGTGTGTACTTTT
TCATCCCAGGTCTGTCGAGTTGCTTATGAAATTATGCAAACACTACATCCTGATGCCTCTGCAAACTTCCATTCTTTAGATGACATCTACTATTTTGGGG
GCCAGAATGCCCACAATCAAATTGCCATTTATGCTCACCAACCCCGAACTGCAGATGAAATTCCCATGGAACCTGGAGATATCATTGGTGTGGCTGGAAA
TCATTGGGATGGCTATTCTAAAGGTGTCAACAGGAAATTGGGAAGGACGGGCCTATATCCCTCCTACAAAGTTCGAGAGAAGATAGAAACGGTCAAGTAC
CCCACATATCCTGAGGCTGAGAAATAAAGCTCAGATGGAAGAGATAAACGACCAAACTCAGTTCGACCAAACTCAGTTCAAACCATTTCAGCCAAACTGT
AGATGAAGAGGGCTCTGATCTAACAAAATAAGGTTATATGAGTAGATACTCTCAGCACCAAGAGCAGCTGGGAACTGACATAGGCTTCAATTGGTGGAAT
TCCTCTTTAACAAGGGCTGCAATGCCCTCATACCCATGCACAGTACAATAATGTACTCACATATAACATGCAAACAGGTTGTTTTCTACTTTGCCCCTTT
CAGTATGTCCCCATAAGACAAACACTGCCATATTGTGTAATTTAAGTGACACAGACATTTTGTGTGAGACTTAAAACATGGTGCCTATATCTGAGAGACC
TGTGTGAACTATTGAGAAGATCGGAACAGCTCCTTACTCTGAGGAAGTTGATTCTTATTTGATGGTGGTATTGTGACCACTGAATTCACTCCAGTCAACA
GATTCAGAATGAGAATGGACGTTTGGTTTTTTTTTGTTTTTGTTTTTGTTTTTTCCTTTATAAGGTTGTCTGTTTTTTTTTTTTTAAATAATTGCATCAG
TTCATTGACCTCATCATTAATAAGTGAAGAATACATCAGAAAATAAAATATTCACTCTCCATTAGAAAATTTTGTAAAACAATGCCATGAACAAATTCTT
TAGTACTCAATGTTTCTGGACATTCTCTTTGATAACAAAAAATAAATTTTAAAAAGGAATTTTGTAAAGTTTCTAGAATTTTATATCATTGGATGATATG
TTGATCAGCCTTATGTGGAAGAACTGTGATAAAAAGAGGAGCTTTTTAGTTTTTCAGCTTA
  • refseq_FUT8.F1 (forward primer)
  • refseq_FUT8.R1 (reverse primer)
  • Single exon skipping

Frame 0

RLVQKSHGREHPCIYKLLLNYFIE**HPN*ART
*FWLC*YIFCVILDKSLYFPFNFLFSQGDNHKND
CKVQHFIECFLFKELTINTSLKV*ESLPHNLSC
LRTRDFLLNQQYLICVSVIEFLVHKKVSINM*I
YEY*ADCNLDRPLPLKSPFCDLLIFNI*KAAATC
FGISIPGMYFKMPKPREMILVLRAPFRSPTVKR
PRLGIWVPRLQGRVWNGKLIFRPSDWPARTPLT
RRAALIGLGGTPRPATTLCAGAARSGPPAQLAVW
AALGQPFGPLLCIAGAGNFPSPSGM*SA*STGQ
*SFLHISPGGSL*KIHCRTTRENNLSEASCVET
TEVYSPVH*LETELQCFQFFELQDSREVS*KSEN
AAMDWFLALDYAHSFCLGDLAVLYRWSLGTR**
PS*SL*PRTVQDSGKA*TLKTTE*RLEANGRIS
PDTRRPY*SGASYRKSTRFRRAAC*GQRTD*KLQ
ETDQKWSGEGS*NPEEED*KWS*RALVFPTE*I
EEIKELRRK*TPKTCR*ISFGFRTS*KVYNDGS
ILPQSDRWSR*LAGKRGQRSDRTGSAENNISSES
QGLQQSQKAGV*YQQRLWLWLSAPSCGLLLHDC
IWHPANTHLGISELALCYWWMGDCI*ACK*DMH
RQIWHLHWTLVR*SEGQKCSSGRASHCRQSSSPS
SIFTLGCTRRPRRSTCTSAW*PCSVVGVSVCQI
LDPPTALARKRNRRSHQEAWLQTSSYWSPCQTH
RQSGNRSCLPSH*RVHGAC*RTFSASCTQNASGQ
KKSVFGHR*PFFIKGGKNKVPQL*IY***LYFL
VSWTAQSIHRKFTSWSDPGYTFSLSGRLPSVYF
FIPGLSSCL*NYANTTS*CLCKLPFFR*HLLFWG
PECPQSNCHLCSPTPNCR*NSHGTWRYHWCGWK
SLGWLF*RCQQEIGKDGPISLLQSSREDRNGQV
PHIS*G*EIKLRWKR*TTKLSSTKLSSNHFSQTV
DEEGSDLTK*GYMSRYSQHQEQLGTDIGFNWWN
SSLTRAAMPSYPCTVQ*CTHI*HANRLFSTLPL
SVCPHKTNTAILCNLSDTDILCET*NMVPISERP
V*TIEKIGTAPYSEEVDSYLMVVL*PLNSLQST
DSE*EWTFGFFLFLFLFFPL*GCLFFFF*IIAS
VH*PHH**VKNTSENKIFTLH*KIL*NNAMNKFF
STQCFWTFSLITKNKF*KGIL*SF*NFISLDDM
LISLMWKNCDKKRSFLVFQL

Frame 1

V*YRNLMGESIHAFTNCY*IILLNDDTQTELEH
NSGSASTSSV*SWTSHSTFLSIFFSHREIIIKT
TVKYSTS*SAFCLKS*Q*IRVSRSRKASLTT*AA
*GQGIFF*ISSTLFVYL**SSWYIRRSQ*ICEF
MNIRQIATLTGHCLLNLLSVIF*YLTSKRPPLL
ALG*VSPVCTLKCPSLEK*FLS*GHHFALPP*SA
PGLGSGSQGYREEFGTGSSSSGPLIGRLALHSR
GAQL*LASAAPLVPRLLCVLGRRAPVLPLSWRS
GLLWGSPSVHCSASRAPGIFRVRAACRAHEVQDN
KASYTYHQEDLFERFTAGLPERIICLKHHVLKQ
QKSIHLCTN*KQSYNVFNSLSSRTPGK*VENLK
MRPWTGSWRWIMLILFAWGTLLFYIGGHLVRDND
HPDHSSRELSKILAKLERLKQQNEDLRRMAESL
RIPEGPIDQGPAIGRVRVLEEQLVKAKEQIENY
KKQTRNGLGKDHEILRRRIENGAKELWFFLQSEL
KKLKNLEGNELQRHADEFLLDLGHHERSIMTDL
YYLSQTDGAGDWREKEAKDLTELVQRRITYLQN
PKDCSKAKKLVCNINKGCGYGCQLHHVVYCFMIA
YGTQRTLILESQNWRYATGGWETVFRPVSETCT
DRSGISTGHWSGEVKDKNVQVVELPIVDSLHPR
PPYLPLAVPEDLADRLVRVHGDPAVWWVSQFVKY
LIRPQPWLEKEIEEATKKLGFKHPVIGVHVRRT
DKVGTEAAFHPIEEYMVHVEEHFQLLARRMQVD
KKRVYLATDDPSLLKEAKTKYPNYEFISDNSISW
SAGLHNRYTENSLRGVILDIHFLSQADFLVCTF
SSQVCRVAYEIMQTLHPDASANFHSLDDIYYFG
GQNAHNQIAIYAHQPRTADEIPMEPGDIIGVAGN
HWDGYSKGVNRKLGRTGLYPSYKVREKIETVKY
PTYPEAEK*SSDGRDKRPNSVRPNSVQTISAKL
*MKRALI*QNKVI*VDTLSTKSSWELT*ASIGGI
PL*QGLQCPHTHAQYNNVLTYNMQTGCFLLCPF
QYVPIRQTLPYCVI*VTQTFCVRLKTWCLYLRD
LCELLRRSEQLLTLRKLILI*WWYCDH*IHSSQQ
IQNENGRLVFFCFCFCFFLYKVVCFFFFK*LHQ
FIDLIINK*RIHQKIKYSLSIRKFCKTMP*TNS
LVLNVSGHSL**QKINFKKEFCKVSRILYHWMIC
*SALCGRTVIKRGAF*FFSL

Frame 2

FSTEISWERASMHLQIVIELFY*MMTPKLS*NI
ILALLVHLLCDLGQVTLLSFQFSFLTGR*S*KR
L*STALHRVLFV*RADNKYESQGLGKPPSQPEL
LEDKGFSFESAVPYLCICDRVPGT*EGLNKYVNL
*ILGRLQP*QATAS*ISFL*SFNI*HLKGRRYL
LWDKYPRYVL*NAQA*RNDSCLKGTISLSHRKA
PQAWDLGPKATGKSLEREAHLPAL*LAGSHSTHA
ARSSDWPRRHPSSRDYFVCWGGALRSSRSAGGL
GCSGAALRSTALHRGRREFSESERHVERMKYRT
IKLPTHITRRISLKDSLQDYQRE*FV*SIMC*NN
RSLFTCALTRNRVTMFSIL*APGLQGSELKI*K
CGHGLVPGVGLCSFFLPGGPCCFI*VVTWYEIM
TILITLAENCPRFWQSLNA*NNRMKT*GEWPNLS
GYQKALLIRGQL*EEYAF*KSSLLRPKNRLKIT
RNRPEMVWGRIMKS*GGGLKMELKSSGFSYRVN
*RN*RT*KEMNSKDMQMNFFWI*DIMKGL**RIY
TTSVRQMEQVIGGKKRPKI*QNWFSGE*HIFRI
PRTAAKPKSWCVISTKAVAMAVSSIMWSTAS*L
HMAPSEHSSWNLRIGAMLLVDGRLYLGL*VRHAQ
TDLASPLDTGQVK*RTKMFKWSSFPL*TVFIPV
LHIYPWLYQKTSQIDLYECMVTLQCGGCLSLSN
T*SAHSLG*KKK*KKPPRSLASNIQLLESMSDAQ
TKWEQKLPSIPLKSTWCMLKNIFSFLHAECKWT
KKECIWPQMTLLY*RRQKQSTPIMNLLVITLFP
GQLDCTIDTQKIHFVE*SWIYIFSLRQTS*CVLF
HPRSVELLMKLCKHYILMPLQTSIL*MTSTILG
ARMPTIKLPFMLTNPELQMKFPWNLEISLVWLE
IIGMAILKVSTGNWEGRAYIPPTKFERR*KRSST
PHILRLRNKAQMEEINDQTQFDQTQFKPFQPNC
R*RGL*SNKIRLYE*ILSAPRAAGN*HRLQLVE
FLFNKGCNALIPMHSTIMYSHITCKQVVFYFAPF
SMSP*DKHCHIV*FK*HRHFV*DLKHGAYI*ET
CVNY*EDRNSSLL*GS*FLFDGGIVTTEFTPVN
RFRMRMDVWFFFVFVFVFSFIRLSVFFFLNNCIS
SLTSSLISEEYIRK*NIHSPLENFVKQCHEQIL
*YSMFLDILFDNKK*ILKRNFVKFLEFYIIG*Y
VDQPYVEEL**KEELFSFSA

Sequence NM_178154

GGCCGACCCGAGCAGCCGGTTCCCTCCTCTCCAGGCCCCCTCCCCATCCCACCCCCGCCGCCTGGCCCCAGCCGACCCGTCCCTTCGTCTCCCCGCGGAA
TGGGGCCGGCACTGCTCAGGGTCGCGCGCCCTGGACCCAGCTCGCTCTCGGTCTCGCGCTGTCAGCGACTGCCCGGCTCGCGCCGCCTCGCGCTCTGCCT
CAGTCAGTGGCGCCGAAGGCTCCGTTAAGCGGCGGCGGCGGTTCCTGTTTCCGTTTCTTCCTCTCCGTTCGGTCGGGAGTAGCATCCTCCACTCAGCCAC
CCTTCCCACTCCCCCATCGTGGGGCAGCTGCGGCTGAGGGCTGTGGCTTTGGCAGCTGCGACGGGGAGCGGCGGAGACCGCCTCTGCTCCCGCCTGGGGT
TGCTGCTTTTGCTCAGAGGACATCCATGACCCTAATGGTCTTTTTGTTCAAGATAAAGTGATTTTTTGCCTTTGTTGATTAACTGGACAAATTCAGCATG
TAGAGCGCATGAAGTACAGGACAATAAAGCTTCCTACACATATCACCAGGAGGATCTCTTTGAAAGATTCACTGCAGGACTACCAGAGAGAATAATTTGT
CTGAAGCATCATGTGTTGAAACAACAGAAGTCTATTCACCTGTGCACTAACTAGAAACAGAGTTACAATGTTTTCAATTCTTTGAGCTCCAGGACTCCAG
GGAAGTGAGTTGAAAATCTGAAAATGCGGCCATGGACTGGTTCCTGGCGTTGGATTATGCTCATTCTTTTTGCCTGGGGGACCTTGCTGTTTTATATAGG
TGGTCACTTGGTACGAGATAATGACCATCCTGATCACTCTAGCCGAGAACTGTCCAAGATTCTGGCAAAGCTTGAACGCTTAAAACAACAGAATGAAGAC
TTGAGGCGAATGGCCGAATCTCTCCGGATACCAGAAGGCCCTATTGATCAGGGGCCAGCTATAGGAAGAGTACGCGTTTTAGAAGAGCAGCTTGTTAAGG
CCAAAGAACAGATTGAAAATTACAAGAAACAGACCAGAAATGGTCTGGGGAAGGATCATGAAATCCTGAGGAGGAGGATTGAAAATGGAGCTAAAGAGCT
CTGGTTTTTCCTACAGAGTGAATTGAAGAAATTAAAGAACTTAGAAGGAAATGAACTCCAAAGACATGCAGATGAATTTCTTTTGGATTTAGGACATCAT
GAAAGGTCTATAATGACGGATCTATACTACCTCAGTCAGACAGATGGAGCAGGTGATTGGCGGGAAAAAGAGGCCAAAGATCTGACAGAACTGGTTCAGC
GGAGAATAACA
TATCTTCAGAATCCCAAGGACTGCAGCAAAGCCAAAAAGCTGGTGTGTAATATCAACAAAGGCTGTGGCTATGGCTGTCAGCTCCATCA
TGTGGTCTACTGCTTCATGATTGCATATGGCACCCAGCGAACACTCATCTTGGAATCTCAGAATTGGCGCTATGCTACTGGTGGATGGGAGACTGTATTT
AGGCCTGTAAGTGAGACATGCACAGACAGATCTGGCATCTCCACTGGACACTGGTCAGGTGAAGTGAAGGACAAAAATGTTCAAGTGGTCGAGCTTCCCA
TTGTAGACAGTCTTCATCCCCGTCCTCCATATTTACCCTTGGCTGTACCAGAAGACCTCGCAGATCGACTTGTACGAGTGCATGGTGACCCTGCAGTGTG
GTGGGTGTCTCAGTTTGTCAAATACTTGATCCGCCCACAGCCTTGGCTAGAAAAAGAAATAGAAGAAGCCACCAAGAAGCTTGGCTTCAAACATCCAGTT
ATTGGAGTCCATGTCAGACGCACAGACAAAGTGGGAACAGAAGCTGCCTTCCATCCCATTGAAGAGTACATGGTGCATGTTGAAGAACATTTTCAGCTTC
TTGCACGCAGAATGCAAGTGGACAAAAAAAGAGTGTATTTGGCCACAGATGACCCTTCTTTATTAAAGGAGGCAAAAACAAAGTACCCCAATTATGAATT
TATTAGTGATAACTCTATTTCCTGGTCAGCTGGACTGCACAATCGATACACAGAAAATTCACTTCGTGGAGTGATCCTGGATATACATTTTCTCTCTCAG
GCAGACTTCCTAGTGTGTACTTTTTCATCCCAGGTCTGTCGAGTTGCTTATGAAATTATGCAAACACTACATCCTGATGCCTCTGCAAACTTCCATTCTT
TAGATGACATCTACTATTTTGGGGGCCAGAATGCCCACAATCAAATTGCCATTTATGCTCACCAACCCCGAACTGCAGATGAAATTCCCATGGAACCTGG
AGATATCATTGGTGTGGCTGGAAATCATTGGGATGGCTATTCTAAAGGTGTCAACAGGAAATTGGGAAGGACGGGCCTATATCCCTCCTACAAAGTTCGA
GAGAAGATAGAAACGGTCAAGTACCCCACATATCCTGAGGCTGAGAAATAAAGCTCAGATGGAAGAGATAAACGACCAAACTCAGTTCGACCAAACTCAG
TTCAAACCATTTCAGCCAAACTGTAGATGAAGAGGGCTCTGATCTAACAAAATAAGGTTATATGAGTAGATACTCTCAGCACCAAGAGCAGCTGGGAACT
GACATAGGCTTCAATTGGTGGAATTCCTCTTTAACAAGGGCTGCAATGCCCTCATACCCATGCACAGTACAATAATGTACTCACATATAACATGCAAACA
GGTTGTTTTCTACTTTGCCCCTTTCAGTATGTCCCCATAAGACAAACACTGCCATATTGTGTAATTTAAGTGACACAGACATTTTGTGTGAGACTTAAAA
CATGGTGCCTATATCTGAGAGACCTGTGTGAACTATTGAGAAGATCGGAACAGCTCCTTACTCTGAGGAAGTTGATTCTTATTTGATGGTGGTATTGTGA
CCACTGAATTCACTCCAGTCAACAGATTCAGAATGAGAATGGACGTTTGGTTTTTTTTTGTTTTTGTTTTTGTTTTTTCCTTTATAAGGTTGTCTGTTTT
TTTTTTTTTAAATAATTGCATCAGTTCATTGACCTCATCATTAATAAGTGAAGAATACATCAGAAAATAAAATATTCACTCTCCATTAGAAAATTTTGTA
AAACAATGCCATGAACAAATTCTTTAGTACTCAATGTTTCTGGACATTCTCTTTGATAACAAAAAATAAATTTTAAAAAGGAATTTTGTAAAGTTTCTAG
AATTTTATATCATTGGATGATATGTTGATCAGCCTTATGTGGAAGAACTGTGATAAAAAGAGGAGCTTTTTAGTTTTTCAGCTTA
  • refseq_FUT8.F1 (forward primer)
  • refseq_FUT8.R1 (reverse primer)
  • Single exon skipping

Frame 0

GRPEQPVPSSPGPLPIPPPPPGPSRPVPSSPRG
MGPALLRVARPGPSSLSVSRCQRLPGSRRLALCL
SQWRRRLR*AAAAVPVSVSSSPFGRE*HPPLSH
PSHSPIVGQLRLRAVALAAATGSGGDRLCSRLG
LLLLLRGHP*P*WSFCSR*SDFLPLLINWTNSAC
RAHEVQDNKASYTYHQEDLFERFTAGLPERIIC
LKHHVLKQQKSIHLCTN*KQSYNVFNSLSSRTP
GK*VENLKMRPWTGSWRWIMLILFAWGTLLFYIG
GHLVRDNDHPDHSSRELSKILAKLERLKQQNED
LRRMAESLRIPEGPIDQGPAIGRVRVLEEQLVK
AKEQIENYKKQTRNGLGKDHEILRRRIENGAKEL
WFFLQSELKKLKNLEGNELQRHADEFLLDLGHH
ERSIMTDLYYLSQTDGAGDWREKEAKDLTELVQ
RRIT
YLQNPKDCSKAKKLVCNINKGCGYGCQLHH
VVYCFMIAYGTQRTLILESQNWRYATGGWETVF
RPVSETCTDRSGISTGHWSGEVKDKNVQVVELP
IVDSLHPRPPYLPLAVPEDLADRLVRVHGDPAVW
WVSQFVKYLIRPQPWLEKEIEEATKKLGFKHPV
IGVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQL
LARRMQVDKKRVYLATDDPSLLKEAKTKYPNYEF
ISDNSISWSAGLHNRYTENSLRGVILDIHFLSQ
ADFLVCTFSSQVCRVAYEIMQTLHPDASANFHS
LDDIYYFGGQNAHNQIAIYAHQPRTADEIPMEPG
DIIGVAGNHWDGYSKGVNRKLGRTGLYPSYKVR
EKIETVKYPTYPEAEK*SSDGRDKRPNSVRPNS
VQTISAKL*MKRALI*QNKVI*VDTLSTKSSWEL
T*ASIGGIPL*QGLQCPHTHAQYNNVLTYNMQT
GCFLLCPFQYVPIRQTLPYCVI*VTQTFCVRLK
TWCLYLRDLCELLRRSEQLLTLRKLILI*WWYCD
H*IHSSQQIQNENGRLVFFCFCFCFFLYKVVCF
FFFK*LHQFIDLIINK*RIHQKIKYSLSIRKFC
KTMP*TNSLVLNVSGHSL**QKINFKKEFCKVSR
ILYHWMIC*SALCGRTVIKRGAF*FFSL

Frame 1

ADPSSRFPPLQAPSPSHPRRLAPADPSLRLPAE
WGRHCSGSRALDPARSRSRAVSDCPARAASRSA
SVSGAEGSVKRRRRFLFPFLPLRSVGSSILHSAT
LPTPPSWGSCG*GLWLWQLRRGAAETASAPAWG
CCFCSEDIHDPNGLFVQDKVIFCLC*LTGQIQH
VERMKYRTIKLPTHITRRISLKDSLQDYQRE*FV
*SIMC*NNRSLFTCALTRNRVTMFSIL*APGLQ
GSELKI*KCGHGLVPGVGLCSFFLPGGPCCFI*
VVTWYEIMTILITLAENCPRFWQSLNA*NNRMKT
*GEWPNLSGYQKALLIRGQL*EEYAF*KSSLLR
PKNRLKITRNRPEMVWGRIMKS*GGGLKMELKS
SGFSYRVN*RN*RT*KEMNSKDMQMNFFWI*DIM
KGL**RIYTTSVRQMEQVIGGKKRPKI*QNWFS
GE*H
IFRIPRTAAKPKSWCVISTKAVAMAVSSI
MWSTAS*LHMAPSEHSSWNLRIGAMLLVDGRLYL
GL*VRHAQTDLASPLDTGQVK*RTKMFKWSSFP
L*TVFIPVLHIYPWLYQKTSQIDLYECMVTLQC
GGCLSLSNT*SAHSLG*KKK*KKPPRSLASNIQL
LESMSDAQTKWEQKLPSIPLKSTWCMLKNIFSF
LHAECKWTKKECIWPQMTLLY*RRQKQSTPIMN
LLVITLFPGQLDCTIDTQKIHFVE*SWIYIFSLR
QTS*CVLFHPRSVELLMKLCKHYILMPLQTSIL
*MTSTILGARMPTIKLPFMLTNPELQMKFPWNL
EISLVWLEIIGMAILKVSTGNWEGRAYIPPTKFE
RR*KRSSTPHILRLRNKAQMEEINDQTQFDQTQ
FKPFQPNCR*RGL*SNKIRLYE*ILSAPRAAGN
*HRLQLVEFLFNKGCNALIPMHSTIMYSHITCKQ
VVFYFAPFSMSP*DKHCHIV*FK*HRHFV*DLK
HGAYI*ETCVNY*EDRNSSLL*GS*FLFDGGIV
TTEFTPVNRFRMRMDVWFFFVFVFVFSFIRLSVF
FFLNNCISSLTSSLISEEYIRK*NIHSPLENFV
KQCHEQIL*YSMFLDILFDNKK*ILKRNFVKFL
EFYIIG*YVDQPYVEEL**KEELFSFSA

Frame 2

PTRAAGSLLSRPPPHPTPAAWPQPTRPFVSPRN
GAGTAQGRAPWTQLALGLALSATARLAPPRALP
QSVAPKAPLSGGGGSCFRFFLSVRSGVASSTQP
PFPLPHRGAAAAEGCGFGSCDGERRRPPLLPPGV
AAFAQRTSMTLMVFLFKIK*FFAFVD*LDKFSM
*SA*STGQ*SFLHISPGGSL*KIHCRTTRENNL
SEASCVETTEVYSPVH*LETELQCFQFFELQDSR
EVS*KSENAAMDWFLALDYAHSFCLGDLAVLYR
WSLGTR**PS*SL*PRTVQDSGKA*TLKTTE*R
LEANGRISPDTRRPY*SGASYRKSTRFRRAAC*G
QRTD*KLQETDQKWSGEGS*NPEEED*KWS*RA
LVFPTE*IEEIKELRRK*TPKTCR*ISFGFRTS
*KVYNDGSILPQSDRWSR*LAGKRGQRSDRTGSA
ENN
ISSESQGLQQSQKAGV*YQQRLWLWLSAPS
CGLLLHDCIWHPANTHLGISELALCYWWMGDCI
*ACK*DMHRQIWHLHWTLVR*SEGQKCSSGRASH
CRQSSSPSSIFTLGCTRRPRRSTCTSAW*PCSV
VGVSVCQILDPPTALARKRNRRSHQEAWLQTSS
YWSPCQTHRQSGNRSCLPSH*RVHGAC*RTFSAS
CTQNASGQKKSVFGHR*PFFIKGGKNKVPQL*I
Y***LYFLVSWTAQSIHRKFTSWSDPGYTFSLS
GRLPSVYFFIPGLSSCL*NYANTTS*CLCKLPFF
R*HLLFWGPECPQSNCHLCSPTPNCR*NSHGTW
RYHWCGWKSLGWLF*RCQQEIGKDGPISLLQSS
REDRNGQVPHIS*G*EIKLRWKR*TTKLSSTKLS
SNHFSQTVDEEGSDLTK*GYMSRYSQHQEQLGT
DIGFNWWNSSLTRAAMPSYPCTVQ*CTHI*HAN
RLFSTLPLSVCPHKTNTAILCNLSDTDILCET*N
MVPISERPV*TIEKIGTAPYSEEVDSYLMVVL*
PLNSLQSTDSE*EWTFGFFLFLFLFFPL*GCLF
FFF*IIASVH*PHH**VKNTSENKIFTLH*KIL*
NNAMNKFFSTQCFWTFSLITKNKF*KGIL*SF*
NFISLDDMLISLMWKNCDKKRSFLVFQL

Sequence NM_178155

CGTTTAGTACAGAAATCTCATGGGAGAGAGCATCCATGCATTTACAAATTGTTATTGAATTATTTTATTGAATGATGACACCCAAACTGAGCTAGAACAT
AATTCTGGCTCTGCTAGTACATCTTCTGTGTGATCTTGGACAAGTCACTCTACTTTCCTTTCAATTTTCTTTTCTCACAGGGAGATAATCATAAAAACGA
CTGTAAAGTACAGCACTTCATAGAGTGCTTTTTGTTTAAAGAGCTGACAATAAATACGAGTCTCAAGGTCTAGGAAAGCCTCCCTCACAACCTGAGCTGC
TTGAGGACAAGGGATTTTCTTTTGAATCAGCAGTACCTTATTTGTGTATCTGTGATAGAGTTCCTGGTACATAAGAAGGTCTCAATAAATATGTGAATTT
ATGAATATTAGGCAGATTGCAACCTTGACAGGCCACTGCCTCTTAAATCTCCTTTCTGTGATCTTTTAATATTTAACATCTAAAAGGCCGCCGCTACTTG
CTTTGGGATAAGTATCCCCGGTATGTACTTTAAAATGCCCAAGCCTAGAGAAATGATTCTTGTCTTAAGGGCACCATTTCGCTCTCCCACCGTAAAGCGC
CCCAGGCTTGGGATCTGGGTCCCAAGGCTACAGGGAAGAGTTTGGAACGGGAAGCTCATCTTCCGGCCCTCTGATTGGCCGGCTCGCACTCCACTCACGC
GGCGCGCAGCTCTGATTGGCCTCGGCGGCACCCCTCGTCCCGCGACTACTTTGTGTGCTGGGGCGGCGCGCTCCGGTCCTCCCGCTCAGCTGGCGGTCTG
GGCTGCTCTGGGGCAGCCCTTCGGTCCACTGCTCTGCATCGCGGGCGCCGGGAATTTTCCGAGTCCGAGCGGGTTGCTGCTTTTGCTCAGAGGACATCCA
TGACCCTAATGGTCTTTTTGTTCAAGATAAAGTGATTTTTTGCCTTTGTTGATTAACTGGACAAATTCAGCATGTAGAGCGCATGAAGTACAGGACAATA
AAGCTTCCTACACATATCACCAGGAGGATCTCTTTGAAAGATTCACTGCAGGACTACCAGAGAGAATAATTTGTCTGAAGCATCATGTGTTGAAACAACA
GAAGTCTATTCACCTGTGCACTAACTAGAAACAGAGTTACAATGTTTTCAATTCTTTGAGCTCCAGGACTCCAGGGAAGTGAGTTGAAAATCTGAAAATG
CGGCCATGGACTGGTTCCTGGCGTTGGATTATGCTCATTCTTTTTGCCTGGGGGACCTTGCTGTTTTATATAGGTGGTCACTTGGTACGAGATAATGACC
ATCCTGATCACTCTAGCCGAGAACTGTCCAAGATTCTGGCAAAGCTTGAACGCTTAAAACAACAGAATGAAGACTTGAGGCGAATGGCCGAATCTCTCCG
GATACCAGAAGGCCCTATTGATCAGGGGCCAGCTATAGGAAGAGTACGCGTTTTAGAAGAGCAGCTTGTTAAGGCCAAAGAACAGATTGAAAATTACAAG
AAACAGACCAGAAATGGTCTGGGGAAGGATCATGAAATCCTGAGGAGGAGGATTGAAAATGGAGCTAAAGAGCTCTGGTTTTTCCTACAGAGTGAATTGA
AGAAATTAAAGAACTTAGAAGGAAATGAACTCCAAAGACATGCAGATGAATTTCTTTTGGATTTAGGACATCATGAAAGGTCTATAATGACGGATCTATA
CTACCTCAGTCAGACAGATGGAGCAGGTGATTGGCGGGAAAAAGAGGCCAAAGATCTGACAGAACTGGTTCAGCGGAGAATAACATATCTTCAGAATCCC
AAGGACTGCAGCAAAGCCAAAAAGCTGGTGTGTAATATCAACAAAGGCTGTGGCTATGGCTGTCAGCTCCATCATGTGGTCTACTGCTTCATGATTGCAT
ATGGCACCCAGCGAACACTCATCTTGGAATCTCAGAATTGGCGCTATGCTACTGGTGGATGGGAGACTGTATTTAGGCCTGTAAGTGAGACATGCACAGA
CAGATCTGGCATCTCCACTGGACACTGGTCAGGTGAAGTGAAGGACAAAAATGTTCAAGTGGTCGAGCTTCCCATTGTAGACAGTCTTCATCCCCGTCCT
CCATATTTACCCTTGGCTGTACCAGAAGACCTCGCAGATCGACTTGTACGAGTGCATGGTGACCCTGCAGTGTGGTGGGTGTCTCAGTTTGTCAAATACT
TGATCCGCCCACAGCCTTGGCTAGAAAAAGAAATAGAAGAAGCCACCAAGAAGCTTGGCTTCAAACATCCAGTTATTGGAGTCCATGTCAGACGCACAGA
CAAAGTGGGAACAGAAGCTGCCTTCCATCCCATTGAAGAGTACATGGTGCATGTTGAAGAACATTTTCAGCTTCTTGCACGCAGAATGCAAGTGGACAAA
AAAAGAGTGTATTTGGCCACAGATGACCCTTCTTTATTAAAGGAGGCAAAAACAAAGTACCCCAATTATGAATTTATTAGTGATAACTCTATTTCCTGGT
CAGCTGGACTGCACAATCGATACACAGAAAATTCACTTCGTGGAGTGATCCTGGATATACATTTTCTCTCTCAGGCAGACTTCCTAGTGTGTACTTTTTC
ATCCCAGGTCTGTCGAGTTGCTTATGAAATTATGCAAACACTACATCCTGATGCCTCTGCAAACTTCCATTCTTTAGATGACATCTACTATTTTGGGGGC
CAGAATGCCCACAATCAAATTGCCATTTATGCTCACCAACCCCGAACTGCAGATGAAATTCCCATGGAACCTGGAGATATCATTGGTGTGGCTGGAAATC
ATTGGGATGGCTATTCTAAAGGTGTCAACAGGAAATTGGGAAGGACGGGCCTATATCCCTCCTACAAAGTTCGAGAGAAGATAGAAACGGTCAAGTACCC
CACATATCCTGAGGCTGAGAAATAAAGCTCAGATGGAAGAGATAAACGACCAAACTCAGTTCGACCAAACTCAGTTCAAACCATTTCAGCCAAACTGTAG
ATGAAGAGGGCTCTGATCTAACAAAATAAGGTTATATGAGTAGATACTCTCAGCACCAAGAGCAGCTGGGAACTGACATAGGCTTCAATTGGTGGAATTC
CTCTTTAACAAGGGCTGCAATGCCCTCATACCCATGCACAGTACAATAATGTACTCACATATAACATGCAAACAGGTTGTTTTCTACTTTGCCCCTTTCA
GTATGTCCCCATAAGACAAACACTGCCATATTGTGTAATTTAAGTGACACAGACATTTTGTGTGAGACTTAAAACATGGTGCCTATATCTGAGAGACCTG
TGTGAACTATTGAGAAGATCGGAACAGCTCCTTACTCTGAGGAAGTTGATTCTTATTTGATGGTGGTATTGTGACCACTGAATTCACTCCAGTCAACAGA
TTCAGAATGAGAATGGACGTTTGGTTTTTTTTTGTTTTTGTTTTTGTTTTTTCCTTTATAAGGTTGTCTGTTTTTTTTTTTTTAAATAATTGCATCAGTT
CATTGACCTCATCATTAATAAGTGAAGAATACATCAGAAAATAAAATATTCACTCTCCATTAGAAAATTTTGTAAAACAATGCCATGAACAAATTCTTTA
GTACTCAATGTTTCTGGACATTCTCTTTGATAACAAAAAATAAATTTTAAAAAGGAATTTTGTAAAGTTTCTAGAATTTTATATCATTGGATGATATGTT
GATCAGCCTTATGTGGAAGAACTGTGATAAAAAGAGGAGCTTTTTAGTTTTTCAGCTTA
  • refseq_FUT8.F1 (forward primer)
  • refseq_FUT8.R1 (reverse primer)
  • Single exon skipping

Frame 0

RLVQKSHGREHPCIYKLLLNYFIE**HPN*ART
*FWLC*YIFCVILDKSLYFPFNFLFSQGDNHKND
CKVQHFIECFLFKELTINTSLKV*ESLPHNLSC
LRTRDFLLNQQYLICVSVIEFLVHKKVSINM*I
YEY*ADCNLDRPLPLKSPFCDLLIFNI*KAAATC
FGISIPGMYFKMPKPREMILVLRAPFRSPTVKR
PRLGIWVPRLQGRVWNGKLIFRPSDWPARTPLT
RRAALIGLGGTPRPATTLCAGAARSGPPAQLAVW
AALGQPFGPLLCIAGAGNFPSPSGLLLLLRGHP
*P*WSFCSR*SDFLPLLINWTNSACRAHEVQDN
KASYTYHQEDLFERFTAGLPERIICLKHHVLKQQ
KSIHLCTN*KQSYNVFNSLSSRTPGK*VENLKM
RPWTGSWRWIMLILFAWGTLLFYIGGHLVRDND
HPDHSSRELSKILAKLERLKQQNEDLRRMAESLR
IPEGPIDQGPAIGRVRVLEEQLVKAKEQIENYK
KQTRNGLGKDHEILRRRIENGAKELWFFLQSEL
KKLKNLEGNELQRHADEFLLDLGHHERSIMTDLY
YLSQTDGAGDWREKEAKDLTELVQRRITYLQNP
KDCSKAKKLVCNINKGCGYGCQLHHVVYCFMIA
YGTQRTLILESQNWRYATGGWETVFRPVSETCTD
RSGISTGHWSGEVKDKNVQVVELPIVDSLHPRP
PYLPLAVPEDLADRLVRVHGDPAVWWVSQFVKY
LIRPQPWLEKEIEEATKKLGFKHPVIGVHVRRTD
KVGTEAAFHPIEEYMVHVEEHFQLLARRMQVDK
KRVYLATDDPSLLKEAKTKYPNYEFISDNSISW
SAGLHNRYTENSLRGVILDIHFLSQADFLVCTFS
SQVCRVAYEIMQTLHPDASANFHSLDDIYYFGG
QNAHNQIAIYAHQPRTADEIPMEPGDIIGVAGN
HWDGYSKGVNRKLGRTGLYPSYKVREKIETVKYP
TYPEAEK*SSDGRDKRPNSVRPNSVQTISAKL*
MKRALI*QNKVI*VDTLSTKSSWELT*ASIGGI
PL*QGLQCPHTHAQYNNVLTYNMQTGCFLLCPFQ
YVPIRQTLPYCVI*VTQTFCVRLKTWCLYLRDL
CELLRRSEQLLTLRKLILI*WWYCDH*IHSSQQ
IQNENGRLVFFCFCFCFFLYKVVCFFFFK*LHQF
IDLIINK*RIHQKIKYSLSIRKFCKTMP*TNSL
VLNVSGHSL**QKINFKKEFCKVSRILYHWMIC
*SALCGRTVIKRGAF*FFSL

Frame 1

V*YRNLMGESIHAFTNCY*IILLNDDTQTELEH
NSGSASTSSV*SWTSHSTFLSIFFSHREIIIKT
TVKYSTS*SAFCLKS*Q*IRVSRSRKASLTT*AA
*GQGIFF*ISSTLFVYL**SSWYIRRSQ*ICEF
MNIRQIATLTGHCLLNLLSVIF*YLTSKRPPLL
ALG*VSPVCTLKCPSLEK*FLS*GHHFALPP*SA
PGLGSGSQGYREEFGTGSSSSGPLIGRLALHSR
GAQL*LASAAPLVPRLLCVLGRRAPVLPLSWRS
GLLWGSPSVHCSASRAPGIFRVRAGCCFCSEDIH
DPNGLFVQDKVIFCLC*LTGQIQHVERMKYRTI
KLPTHITRRISLKDSLQDYQRE*FV*SIMC*NN
RSLFTCALTRNRVTMFSIL*APGLQGSELKI*KC
GHGLVPGVGLCSFFLPGGPCCFI*VVTWYEIMT
ILITLAENCPRFWQSLNA*NNRMKT*GEWPNLS
GYQKALLIRGQL*EEYAF*KSSLLRPKNRLKITR
NRPEMVWGRIMKS*GGGLKMELKSSGFSYRVN*
RN*RT*KEMNSKDMQMNFFWI*DIMKGL**RIY
TTSVRQMEQVIGGKKRPKI*QNWFSGE*HIFRIP
RTAAKPKSWCVISTKAVAMAVSSIMWSTAS*LH
MAPSEHSSWNLRIGAMLLVDGRLYLGL*VRHAQ
TDLASPLDTGQVK*RTKMFKWSSFPL*TVFIPVL
HIYPWLYQKTSQIDLYECMVTLQCGGCLSLSNT
*SAHSLG*KKK*KKPPRSLASNIQLLESMSDAQ
TKWEQKLPSIPLKSTWCMLKNIFSFLHAECKWTK
KECIWPQMTLLY*RRQKQSTPIMNLLVITLFPG
QLDCTIDTQKIHFVE*SWIYIFSLRQTS*CVLF
HPRSVELLMKLCKHYILMPLQTSIL*MTSTILGA
RMPTIKLPFMLTNPELQMKFPWNLEISLVWLEI
IGMAILKVSTGNWEGRAYIPPTKFERR*KRSST
PHILRLRNKAQMEEINDQTQFDQTQFKPFQPNCR
*RGL*SNKIRLYE*ILSAPRAAGN*HRLQLVEF
LFNKGCNALIPMHSTIMYSHITCKQVVFYFAPF
SMSP*DKHCHIV*FK*HRHFV*DLKHGAYI*ETC
VNY*EDRNSSLL*GS*FLFDGGIVTTEFTPVNR
FRMRMDVWFFFVFVFVFSFIRLSVFFFLNNCIS
SLTSSLISEEYIRK*NIHSPLENFVKQCHEQIL*
YSMFLDILFDNKK*ILKRNFVKFLEFYIIG*YV
DQPYVEEL**KEELFSFSA

Frame 2

FSTEISWERASMHLQIVIELFY*MMTPKLS*NI
ILALLVHLLCDLGQVTLLSFQFSFLTGR*S*KR
L*STALHRVLFV*RADNKYESQGLGKPPSQPEL
LEDKGFSFESAVPYLCICDRVPGT*EGLNKYVNL
*ILGRLQP*QATAS*ISFL*SFNI*HLKGRRYL
LWDKYPRYVL*NAQA*RNDSCLKGTISLSHRKA
PQAWDLGPKATGKSLEREAHLPAL*LAGSHSTHA
ARSSDWPRRHPSSRDYFVCWGGALRSSRSAGGL
GCSGAALRSTALHRGRREFSESERVAAFAQRTS
MTLMVFLFKIK*FFAFVD*LDKFSM*SA*STGQ*
SFLHISPGGSL*KIHCRTTRENNLSEASCVETT
EVYSPVH*LETELQCFQFFELQDSREVS*KSEN
AAMDWFLALDYAHSFCLGDLAVLYRWSLGTR**P
S*SL*PRTVQDSGKA*TLKTTE*RLEANGRISP
DTRRPY*SGASYRKSTRFRRAAC*GQRTD*KLQ
ETDQKWSGEGS*NPEEED*KWS*RALVFPTE*IE
EIKELRRK*TPKTCR*ISFGFRTS*KVYNDGSI
LPQSDRWSR*LAGKRGQRSDRTGSAENNISSES
QGLQQSQKAGV*YQQRLWLWLSAPSCGLLLHDCI
WHPANTHLGISELALCYWWMGDCI*ACK*DMHR
QIWHLHWTLVR*SEGQKCSSGRASHCRQSSSPS
SIFTLGCTRRPRRSTCTSAW*PCSVVGVSVCQIL
DPPTALARKRNRRSHQEAWLQTSSYWSPCQTHR
QSGNRSCLPSH*RVHGAC*RTFSASCTQNASGQ
KKSVFGHR*PFFIKGGKNKVPQL*IY***LYFLV
SWTAQSIHRKFTSWSDPGYTFSLSGRLPSVYFF
IPGLSSCL*NYANTTS*CLCKLPFFR*HLLFWG
PECPQSNCHLCSPTPNCR*NSHGTWRYHWCGWKS
LGWLF*RCQQEIGKDGPISLLQSSREDRNGQVP
HIS*G*EIKLRWKR*TTKLSSTKLSSNHFSQTV
DEEGSDLTK*GYMSRYSQHQEQLGTDIGFNWWNS
SLTRAAMPSYPCTVQ*CTHI*HANRLFSTLPLS
VCPHKTNTAILCNLSDTDILCET*NMVPISERP
V*TIEKIGTAPYSEEVDSYLMVVL*PLNSLQSTD
SE*EWTFGFFLFLFLFFPL*GCLFFFF*IIASV
H*PHH**VKNTSENKIFTLH*KIL*NNAMNKFF
STQCFWTFSLITKNKF*KGIL*SF*NFISLDDML
ISLMWKNCDKKRSFLVFQL

Sequence NM_004480

GGCCGACCCGAGCAGCCGGTTCCCTCCTCTCCAGGCCCCCTCCCCATCCCACCCCCGCCGCCTGGCCCCAGCCGACCCGTCCCTTCGTCTCCCCGCGGAA
TGGGGCCGGCACTGCTCAGGGTCGCGCGCCCTGGACCCAGCTCGCTCTCGGTCTCGCGCTGTCAGCGACTGCCCGGCTCGCGCCGCCTCGCGCTCTGCCT
CAGTCAGTGGCGCCGAAGGCTCCGTTAAGCGGCGGCGGCGGTTCCTGTTTCCGTTTCTTCCTCTCCGTTCGGTCGGGAGTAGCATCCTCCACTCAGCCAC
CCTTCCCACTCCCCCATCGTGGGGCAGCTGCGGCTGAGGGCTGTGGCTTTGGCAGCTGCGACGGGGAGCGGCGGAGACCGCCTCTGCTCCCGCCTGGGGT
TGCTGCTTTTGCTCAGAGGACATCCATGACCCTAATGGTCTTTTTGTTCAAGATAAAGTGATTTTTTGCCTTTGTTGATTAACTGGACAAATTCAGGATA
CCAGAAGGCCCTATTGATCAGGGGCCAGCTATAGGAAGAGTACGCGTTTTAGAAGAGCAGCTTGTTAAGGCCAAAGAACAGATTGAAAATTACAAGAAAC
AGACCAGAAATGGTCTGGGGAAGGATCATGAAATCCTGAGGAGGAGGATTGAAAATGGAGCTAAAGAGCTCTGGTTTTTCCTACAGAGTGAATTGAAGAA
ATTAAAGAACTTAGAAGGAAATGAACTCCAAAGACATGCAGATGAATTTCTTTTGGATTTAGGACATCATGAAAGGATTCTGATGGCAATTACTGTCTCA
TTAGTGAACAATAAAAGAAAAATTGTTGTATTAGCACAACCTACTACTGTGAAGAGGAAAAGAATTACCCCATACAA
GTCTATAATGACGGATCTATACT
ACCTCAGTCAGACAGATGGAGCAGGTGATTGGCGGGAAAAAGAGGCCAAAGATCTGACAGAACTGGTTCAGCGGAGAATAACATATCTTCAGAATCCCAA
GGACTGCAGCAAAGCCAAAAAGCTGGTGTGTAATATCAACAAAGGCTGTGGCTATGGCTGTCAGCTCCATCATGTGGTCTACTGCTTCATGATTGCATAT
GGCACCCAGCGAACACTCATCTTGGAATCTCAGAATTGGCGCTATGCTACTGGTGGATGGGAGACTGTATTTAGGCCTGTAAGTGAGACATGCACAGACA
GATCTGGCATCTCCACTGGACACTGGTCAGGTGAAGTGAAGGACAAAAATGTTCAAGTGGTCGAGCTTCCCATTGTAGACAGTCTTCATCCCCGTCCTCC
ATATTTACCCTTGGCTGTACCAGAAGACCTCGCAGATCGACTTGTACGAGTGCATGGTGACCCTGCAGTGTGGTGGGTGTCTCAGTTTGTCAAATACTTG
ATCCGCCCACAGCCTTGGCTAGAAAAAGAAATAGAAGAAGCCACCAAGAAGCTTGGCTTCAAACATCCAGTTATTGGAGTCCATGTCAGACGCACAGACA
AAGTGGGAACAGAAGCTGCCTTCCATCCCATTGAAGAGTACATGGTGCATGTTGAAGAACATTTTCAGCTTCTTGCACGCAGAATGCAAGTGGACAAAAA
AAGAGTGTATTTGGCCACAGATGACCCTTCTTTATTAAAGGAGGCAAAAACAAAGTACCCCAATTATGAATTTATTAGTGATAACTCTATTTCCTGGTCA
GCTGGACTGCACAATCGATACACAGAAAATTCACTTCGTGGAGTGATCCTGGATATACATTTTCTCTCTCAGGCAGACTTCCTAGTGTGTACTTTTTCAT
CCCAGGTCTGTCGAGTTGCTTATGAAATTATGCAAACACTACATCCTGATGCCTCTGCAAACTTCCATTCTTTAGATGACATCTACTATTTTGGGGGCCA
GAATGCCCACAATCAAATTGCCATTTATGCTCACCAACCCCGAACTGCAGATGAAATTCCCATGGAACCTGGAGATATCATTGGTGTGGCTGGAAATCAT
TGGGATGGCTATTCTAAAGGTGTCAACAGGAAATTGGGAAGGACGGGCCTATATCCCTCCTACAAAGTTCGAGAGAAGATAGAAACGGTCAAGTACCCCA
CATATCCTGAGGCTGAGAAATAAAGCTCAGATGGAAGAGATAAACGACCAAACTCAGTTCGACCAAACTCAGTTCAAACCATTTCAGCCAAACTGTAGAT
GAAGAGGGCTCTGATCTAACAAAATAAGGTTATATGAGTAGATACTCTCAGCACCAAGAGCAGCTGGGAACTGACATAGGCTTCAATTGGTGGAATTCCT
CTTTAACAAGGGCTGCAATGCCCTCATACCCATGCACAGTACAATAATGTACTCACATATAACATGCAAACAGGTTGTTTTCTACTTTGCCCCTTTCAGT
ATGTCCCCATAAGACAAACACTGCCATATTGTGTAATTTAAGTGACACAGACATTTTGTGTGAGACTTAAAACATGGTGCCTATATCTGAGAGACCTGTG
TGAACTATTGAGAAGATCGGAACAGCTCCTTACTCTGAGGAAGTTGATTCTTATTTGATGGTGGTATTGTGACCACTGAATTCACTCCAGTCAACAGATT
CAGAATGAGAATGGACGTTTGGTTTTTTTTTGTTTTTGTTTTTGTTTTTTCCTTTATAAGGTTGTCTGTTTTTTTTTTTTTAAATAATTGCATCAGTTCA
TTGACCTCATCATTAATAAGTGAAGAATACATCAGAAAATAAAATATTCACTCTCCATTAGAAAATTTTGTAAAACAATGCCATGAACAAATTCTTTAGT
ACTCAATGTTTCTGGACATTCTCTTTGATAACAAAAAATAAATTTTAAAAAGGAATTTTGTAAAGTTTCTAGAATTTTATATCATTGGATGATATGTTGA
TCAGCCTTATGTGGAAGAACTGTGATAAAAAGAGGAGCTTTTTAGTTTTTCAGCTTA
  • refseq_FUT8.F1 (forward primer)
  • refseq_FUT8.R1 (reverse primer)
  • Single exon skipping

Frame 0

GRPEQPVPSSPGPLPIPPPPPGPSRPVPSSPRG
MGPALLRVARPGPSSLSVSRCQRLPGSRRLALCL
SQWRRRLR*AAAAVPVSVSSSPFGRE*HPPLSH
PSHSPIVGQLRLRAVALAAATGSGGDRLCSRLG
LLLLLRGHP*P*WSFCSR*SDFLPLLINWTNSGY
QKALLIRGQL*EEYAF*KSSLLRPKNRLKITRN
RPEMVWGRIMKS*GGGLKMELKSSGFSYRVN*R
N*RT*KEMNSKDMQMNFFWI*DIMKGF*WQLLSH
**TIKEKLLY*HNLLL*RGKELPHT
SL**RIYT
TSVRQMEQVIGGKKRPKI*QNWFSGE*HIFRIP
RTAAKPKSWCVISTKAVAMAVSSIMWSTAS*LHM
APSEHSSWNLRIGAMLLVDGRLYLGL*VRHAQT
DLASPLDTGQVK*RTKMFKWSSFPL*TVFIPVL
HIYPWLYQKTSQIDLYECMVTLQCGGCLSLSNT*
SAHSLG*KKK*KKPPRSLASNIQLLESMSDAQT
KWEQKLPSIPLKSTWCMLKNIFSFLHAECKWTK
KECIWPQMTLLY*RRQKQSTPIMNLLVITLFPGQ
LDCTIDTQKIHFVE*SWIYIFSLRQTS*CVLFH
PRSVELLMKLCKHYILMPLQTSIL*MTSTILGA
RMPTIKLPFMLTNPELQMKFPWNLEISLVWLEII
GMAILKVSTGNWEGRAYIPPTKFERR*KRSSTP
HILRLRNKAQMEEINDQTQFDQTQFKPFQPNCR
*RGL*SNKIRLYE*ILSAPRAAGN*HRLQLVEFL
FNKGCNALIPMHSTIMYSHITCKQVVFYFAPFS
MSP*DKHCHIV*FK*HRHFV*DLKHGAYI*ETC
VNY*EDRNSSLL*GS*FLFDGGIVTTEFTPVNRF
RMRMDVWFFFVFVFVFSFIRLSVFFFLNNCISS
LTSSLISEEYIRK*NIHSPLENFVKQCHEQIL*
YSMFLDILFDNKK*ILKRNFVKFLEFYIIG*YVD
QPYVEEL**KEELFSFSA

Frame 1

ADPSSRFPPLQAPSPSHPRRLAPADPSLRLPAE
WGRHCSGSRALDPARSRSRAVSDCPARAASRSA
SVSGAEGSVKRRRRFLFPFLPLRSVGSSILHSAT
LPTPPSWGSCG*GLWLWQLRRGAAETASAPAWG
CCFCSEDIHDPNGLFVQDKVIFCLC*LTGQIQD
TRRPY*SGASYRKSTRFRRAAC*GQRTD*KLQET
DQKWSGEGS*NPEEED*KWS*RALVFPTE*IEE
IKELRRK*TPKTCR*ISFGFRTS*KDSDGNYCL
ISEQ*KKNCCISTTYYCEEEKNYPIQ
VYNDGSIL
PQSDRWSR*LAGKRGQRSDRTGSAENNISSESQ
GLQQSQKAGV*YQQRLWLWLSAPSCGLLLHDCI
WHPANTHLGISELALCYWWMGDCI*ACK*DMHRQ
IWHLHWTLVR*SEGQKCSSGRASHCRQSSSPSS
IFTLGCTRRPRRSTCTSAW*PCSVVGVSVCQIL
DPPTALARKRNRRSHQEAWLQTSSYWSPCQTHRQ
SGNRSCLPSH*RVHGAC*RTFSASCTQNASGQK
KSVFGHR*PFFIKGGKNKVPQL*IY***LYFLV
SWTAQSIHRKFTSWSDPGYTFSLSGRLPSVYFFI
PGLSSCL*NYANTTS*CLCKLPFFR*HLLFWGP
ECPQSNCHLCSPTPNCR*NSHGTWRYHWCGWKS
LGWLF*RCQQEIGKDGPISLLQSSREDRNGQVPH
IS*G*EIKLRWKR*TTKLSSTKLSSNHFSQTVD
EEGSDLTK*GYMSRYSQHQEQLGTDIGFNWWNS
SLTRAAMPSYPCTVQ*CTHI*HANRLFSTLPLSV
CPHKTNTAILCNLSDTDILCET*NMVPISERPV
*TIEKIGTAPYSEEVDSYLMVVL*PLNSLQSTD
SE*EWTFGFFLFLFLFFPL*GCLFFFF*IIASVH
*PHH**VKNTSENKIFTLH*KIL*NNAMNKFFS
TQCFWTFSLITKNKF*KGIL*SF*NFISLDDML
ISLMWKNCDKKRSFLVFQL

Frame 2

PTRAAGSLLSRPPPHPTPAAWPQPTRPFVSPRN
GAGTAQGRAPWTQLALGLALSATARLAPPRALP
QSVAPKAPLSGGGGSCFRFFLSVRSGVASSTQP
PFPLPHRGAAAAEGCGFGSCDGERRRPPLLPPGV
AAFAQRTSMTLMVFLFKIK*FFAFVD*LDKFRI
PEGPIDQGPAIGRVRVLEEQLVKAKEQIENYKK
QTRNGLGKDHEILRRRIENGAKELWFFLQSELKK
LKNLEGNELQRHADEFLLDLGHHERILMAITVS
LVNNKRKIVVLAQPTTVKRKRITPYK
SIMTDLY
YLSQTDGAGDWREKEAKDLTELVQRRITYLQNPK
DCSKAKKLVCNINKGCGYGCQLHHVVYCFMIAY
GTQRTLILESQNWRYATGGWETVFRPVSETCTD
RSGISTGHWSGEVKDKNVQVVELPIVDSLHPRPP
YLPLAVPEDLADRLVRVHGDPAVWWVSQFVKYL
IRPQPWLEKEIEEATKKLGFKHPVIGVHVRRTD
KVGTEAAFHPIEEYMVHVEEHFQLLARRMQVDKK
RVYLATDDPSLLKEAKTKYPNYEFISDNSISWS
AGLHNRYTENSLRGVILDIHFLSQADFLVCTFS
SQVCRVAYEIMQTLHPDASANFHSLDDIYYFGGQ
NAHNQIAIYAHQPRTADEIPMEPGDIIGVAGNH
WDGYSKGVNRKLGRTGLYPSYKVREKIETVKYP
TYPEAEK*SSDGRDKRPNSVRPNSVQTISAKL*M
KRALI*QNKVI*VDTLSTKSSWELT*ASIGGIP
L*QGLQCPHTHAQYNNVLTYNMQTGCFLLCPFQ
YVPIRQTLPYCVI*VTQTFCVRLKTWCLYLRDLC
ELLRRSEQLLTLRKLILI*WWYCDH*IHSSQQI
QNENGRLVFFCFCFCFFLYKVVCFFFFK*LHQF
IDLIINK*RIHQKIKYSLSIRKFCKTMP*TNSLV
LNVSGHSL**QKINFKKEFCKVSRILYHWMIC*
SALCGRTVIKRGAF*FFSL

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