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Sequences

Sequence NM_001010850

CGGGGCTGCTCAGTCCTCCAGGCGTCGGTACTCAGCGGTGTTGGAACTTCGTTGCTTGCTTGCCTGTGCGCGCGTGCGCGGACATGGCCTCAAACGATTA
TACCCAACAAGCAACCCAAAGCTATGGGGCCTACCCCACCCAGCCCGGGCAGGGCTATTCCCAGCAGAGCAGTCAGCCCTACGGACAGCAGAGTTACAGT
GGTTATAGCCAGTCCACGGACACTTCAGGCTATGGCCAGAGCAGCTATTCTTCTTATGGCCAGAGCCAGAACACAGGCTATGGAACTCAGTCAACTCCCC
AGGGATATGGCTCGACTGGCGGCTATGGCAGTAGCCAGAGCTCCCAATCGTCTTACGGGCAGCAGTCCTCCTACCCTGGCTATGGCCAGCAGCCAGCTCC
CAGCAGCACCTCGGGAAGTTACGGTAGCAGTTCTCAGAGCAGCAGCTATGGGCAGCCCCAGAGTGGGAGCTACAGCCAGCAGCCTAGCTATGGTGGACAG
CAGCAAAGCTATGGACAGCAGCAAAGCTATAATCCCCCTCAGGGCTATGGACAGCAGAACCAGTACAACAGCAGCAGTGGTGGTGGAGGTGGAGGTGGAG
GTGGAGGTAACTATGGCCAAGATCAATCCTCCATGAGTAGTGGTGGTGGCAGTGGTGGCGGTTATGGCAATCAAGACCAGAGTGGTGGAGGTGGCAGCGG
TGGCTATGGACAGCAGGACCGTGGAGGCCGCGGCAGGGGTGGCAGTGGTGGCGGCGGCGGCGGCGGCGGTGGTGGTTACAACCGCAGCAGTGGTGGCTAT
GAACCCAGAGGTCGTGGAGGTGGCCGTGGAGGCAGAGGTGGCATGGGGCCCTCGGGACCAAGGATCACGTCATGACTCCGAACAGGATAATTCAGACAAC
AACACCATCTTTGTGCAAGGCCTGGGTGAGAATGTTACAATTGAGTCTGTGGCTGATTACTTCAAGCAGATTGGTATTATTAAGACAAACAAGAAAACGG
GACAGCCCATGATTAATTTGTACACAGACAGGGAAACTGGCAAGCTGAAGGGAGAGGCAACGGTCTCTTTTGATGACCCACCTTCAGCTAAAGCAGCTAT
TGACTGGTTTGATGGTAAAGAATTCTCCGGAAATCCTATCAAGGTCTCATTTGCTACTCGCCGGGCAGACTTTAATCGGGGTGGTGGCAATGGTCGTGGA
GGCCGAGGGCGAGGAGGACCCATGGGCCGTGGAGGCTATGGAGGTGGTGGCAGTGGTGGTGGTGGCCGAGGAGGATTTCCCAGTGGAGGTGGTGGCGGTG
GAGGACAGCAGCGAGCTGGTGACTGGAAGTGTCCTAATCCCACCTGTGAGAATATGAACTTCTCTTGGAGGAATGAATGCAACCAGTGTAAGGCCCCTAA
ACCAGATGGCCCAGGAGGGGGACCAGGTGGCTCTCACATGGGGGGTAACTACGGGGATGATCGTCGTGGTGGCAGAGGAGGCTATGATCGAGGCGGCTAC
CGGGGCCGCGGCGGGGACCGTGGAGGCTTCCGAGGGGGCCGGGGTGGTGGGGACAGAGGTGGCTTTGGCCCTGGCAAGATGGATTCCAGGGGTGAGCACA
GACAGGATCGCAGGGAGAGGCCGTATTAATTAGCCTGGCTCCCCAGGTTCTGGAACAGCTTTTTGTCCTGTACCCAGTGTTACCCTCGTTATTTTGTAAC
CTTCCAATTCCTGATCACCCAAGGGTTTTTTTGTGTCGGACTATGTAATTGTAACTATACCTCTGGTTCCCATTAAAAGTGACCATTTTAGTTAAATTTT
GTTCCTCTTCCCCCTTTTCACTTTCCTGGAAGATCGATGTCCCGATCAGGAAGGTAGAGAGTTTTCCTGTTCAGATTACCCTGCCCAGCAGGAACTGGAA
TACAGTGTTCGGGGAGAAGGCCAAATGATATCCTTGAGAGCAGAGATTAAACTTTTCTGTCATGGGGAAA
  • refseq_FUS.F2 (forward primer)
  • refseq_FUS.R2 (reverse primer)
  • Single exon skipping

Frame 0

RGCSVLQASVLSGVGTSLLACLCARARTWPQTI
IPNKQPKAMGPTPPSPGRAIPSRAVSPTDSRVTV
VIASPRTLQAMARAAILLMARARTQAMELSQLP
RDMARLAAMAVARAPNRLTGSSPPTLAMASSQL
PAAPREVTVAVLRAAAMGSPRVGATASSLAMVDS
SKAMDSSKAIIPLRAMDSRTSTTAAVVVEVEVE
VEVTMAKINPP*VVVVAVVAVMAIKTRVVEVAA
VAMDSRTVEAAAGVAVVAAAAAAVVVTTAAVVAM
NPEVVEVAVEAEVAWGPRDQGSRHDSEQDNSDN
NTIFVQGLGENVTIESVADYFKQIGIIKTNKKT
GQPMINLYTDRETGKLKGEATVSFDDPPSAKAAI
DWFDGKEFSGNPIKVSFATRRADFNRGGGNGRG
GRGRGGPMGRGGYGGGGSGGGGRGGFPSGGGGG
GGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPK
PDGPGGGPGGSHMGGNYGDDRRGGRGGYDRGGY
RGRGGDRGGFRGGRGGGDRGGFGPGKMDSRGEH
RQDRRERPY*LAWLPRFWNSFLSCTQCYPRYFVT
FQFLITQGFFCVGLCNCNYTSGSH*K*PF*LNF
VPLPPFHFPGRSMSRSGR*RVFLFRLPCPAGTG
IQCSGRRPNDILESRD*TFLSWG

Frame 1

GAAQSSRRRYSAVLELRCLLACARVRGHGLKRL
YPTSNPKLWGLPHPARAGLFPAEQSALRTAELQ
WL*PVHGHFRLWPEQLFFLWPEPEHRLWNSVNSP
GIWLDWRLWQ*PELPIVLRAAVLLPWLWPAASS
QQHLGKLR*QFSEQQLWAAPEWELQPAA*LWWT
AAKLWTAAKL*SPSGLWTAEPVQQQQWWWRWRWR
WR*LWPRSILHE*WWWQWWRLWQSRPEWWRWQR
WLWTAGPWRPRQGWQWWRRRRRRWWLQPQQWWL
*TQRSWRWPWRQRWHGALGTKDHVMTPNRIIQTT
TPSLCKAWVRMLQLSLWLITSSRLVLLRQTRKR
DSP*LICTQTGKLAS*RERQRSLLMTHLQLKQL
LTGLMVKNSPEILSRSHLLLAGQTLIGVVAMVVE
AEGEEDPWAVEAMEVVAVVVVAEEDFPVEVVAV
EDSSELVTGSVLIPPVRI*TSLGGMNATSVRPL
NQMAQEGDQVALTWGVTTGMIVVVAEEAMIEAAT
GAAAGTVEASEGAGVVGTEVALALARWIPGVST
DRIAGRGRIN*PGSPGSGTAFCPVPSVTLVIL*
PSNS*SPKGFFVSDYVIVTIPLVPIKSDHFS*IL
FLFPLFTFLEDRCPDQEGREFSCSDYPAQQELE
YSVRGEGQMISLRAEIKLFCHGE

Frame 2

GLLSPPGVGTQRCWNFVACLPVRACADMASNDY
TQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYS
GYSQSTDTSGYGQSSYSSYGQSQNTGYGTQSTP
QGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPAP
SSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQ
QQSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGG
GGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSG
GYGQQDRGGRGRGGSGGGGGGGGGGYNRSSGGY
EPRGRGGGRGGRGGMGPSGPRITS*LRTG*FRQ
QHHLCARPG*ECYN*VCG*LLQADWYY*DKQENG
TAHD*FVHRQGNWQAEGRGNGLF**PTFS*SSY
*LV*W*RILRKSYQGLICYSPGRL*SGWWQWSW
RPRARRTHGPWRLWRWWQWWWWPRRISQWRWWRW
RTAASW*LEVS*SHL*EYELLLEE*MQPV*GP*
TRWPRRGTRWLSHGG*LRG*SSWWQRRL*SRRL
PGPRRGPWRLPRGPGWWGQRWLWPWQDGFQG*AQ
TGSQGEAVLISLAPQVLEQLFVLYPVLPSLFCN
LPIPDHPRVFLCRTM*L*LYLWFPLKVTILVKF
CSSSPFSLSWKIDVPIRKVESFPVQITLPSRNWN
TVFGEKAK*YP*EQRLNFSVMGK

Sequence NM_004960

CGGGGCTGCTCAGTCCTCCAGGCGTCGGTACTCAGCGGTGTTGGAACTTCGTTGCTTGCTTGCCTGTGCGCGCGTGCGCGGACATGGCCTCAAACGATTA
TACCCAACAAGCAACCCAAAGCTATGGGGCCTACCCCACCCAGCCCGGGCAGGGCTATTCCCAGCAGAGCAGTCAGCCCTACGGACAGCAGAGTTACAGT
GGTTATAGCCAGTCCACGGACACTTCAGGCTATGGCCAGAGCAGCTATTCTTCTTATGGCCAGAGCCAGAACACAGGCTATGGAACTCAGTCAACTCCCC
AGGGATATGGCTCGACTGGCGGCTATGGCAGTAGCCAGAGCTCCCAATCGTCTTACGGGCAGCAGTCCTCCTACCCTGGCTATGGCCAGCAGCCAGCTCC
CAGCAGCACCTCGGGAAGTTACGGTAGCAGTTCTCAGAGCAGCAGCTATGGGCAGCCCCAGAGTGGGAGCTACAGCCAGCAGCCTAGCTATGGTGGACAG
CAGCAAAGCTATGGACAGCAGCAAAGCTATAATCCCCCTCAGGGCTATGGACAGCAGAACCAGTACAACAGCAGCAGTGGTGGTGGAGGTGGAGGTGGAG
GTGGAGGTAACTATGGCCAAGATCAATCCTCCATGAGTAGTGGTGGTGGCAGTGGTGGCGGTTATGGCAATCAAGACCAGAGTGGTGGAGGTGGCAGCGG
TGGCTATGGACAGCAGGACCGTGGAGGCCGCGGCAGGGGTGGCAGTGGTGGCGGCGGCGGCGGCGGCGGTGGTGGTTACAACCGCAGCAGTGGTGGCTAT
GAACCCAGAGGTCGTGGAGGTGGCCGTGGAGGCAGAGGTGGCATGGGCGGAAGTGACCGTGGTGGCTTCAATAAATTTGGTGGCCCTCGGGACCAAGGAT
CACGTCATGACTCCGAACAGGATAATTCAGACAACAACACCATCTTTGTGCAAGGCCTGGGTGAGAATGTTACAATTGAGTCTGTGGCTGATTACTTCAA
GCAGATTGGTATTATTAAGACAAACAAGAAAACGGGACAGCCCATGATTAATTTGTACACAGACAGGGAAACTGGCAAGCTGAAGGGAGAGGCAACGGTC
TCTTTTGATGACCCACCTTCAGCTAAAGCAGCTATTGACTGGTTTGATGGTAAAGAATTCTCCGGAAATCCTATCAAGGTCTCATTTGCTACTCGCCGGG
CAGACTTTAATCGGGGTGGTGGCAATGGTCGTGGAGGCCGAGGGCGAGGAGGACCCATGGGCCGTGGAGGCTATGGAGGTGGTGGCAGTGGTGGTGGTGG
CCGAGGAGGATTTCCCAGTGGAGGTGGTGGCGGTGGAGGACAGCAGCGAGCTGGTGACTGGAAGTGTCCTAATCCCACCTGTGAGAATATGAACTTCTCT
TGGAGGAATGAATGCAACCAGTGTAAGGCCCCTAAACCAGATGGCCCAGGAGGGGGACCAGGTGGCTCTCACATGGGGGGTAACTACGGGGATGATCGTC
GTGGTGGCAGAGGAGGCTATGATCGAGGCGGCTACCGGGGCCGCGGCGGGGACCGTGGAGGCTTCCGAGGGGGCCGGGGTGGTGGGGACAGAGGTGGCTT
TGGCCCTGGCAAGATGGATTCCAGGGGTGAGCACAGACAGGATCGCAGGGAGAGGCCGTATTAATTAGCCTGGCTCCCCAGGTTCTGGAACAGCTTTTTG
TCCTGTACCCAGTGTTACCCTCGTTATTTTGTAACCTTCCAATTCCTGATCACCCAAGGGTTTTTTTGTGTCGGACTATGTAATTGTAACTATACCTCTG
GTTCCCATTAAAAGTGACCATTTTAGTTAAATTTTGTTCCTCTTCCCCCTTTTCACTTTCCTGGAAGATCGATGTCCCGATCAGGAAGGTAGAGAGTTTT
CCTGTTCAGATTACCCTGCCCAGCAGGAACTGGAATACAGTGTTCGGGGAGAAGGCCAAATGATATCCTTGAGAGCAGAGATTAAACTTTTCTGTCATGG
GGAAA
  • refseq_FUS.F2 (forward primer)
  • refseq_FUS.R2 (reverse primer)
  • Single exon skipping

Frame 0

RGCSVLQASVLSGVGTSLLACLCARARTWPQTI
IPNKQPKAMGPTPPSPGRAIPSRAVSPTDSRVTV
VIASPRTLQAMARAAILLMARARTQAMELSQLP
RDMARLAAMAVARAPNRLTGSSPPTLAMASSQL
PAAPREVTVAVLRAAAMGSPRVGATASSLAMVDS
SKAMDSSKAIIPLRAMDSRTSTTAAVVVEVEVE
VEVTMAKINPP*VVVVAVVAVMAIKTRVVEVAA
VAMDSRTVEAAAGVAVVAAAAAAVVVTTAAVVAM
NPEVVEVAVEAEVAWAEVTVVASINLVALGTKD
HVMTPNRIIQTTTPSLCKAWVRMLQLSLWLITS
SRLVLLRQTRKRDSP*LICTQTGKLAS*RERQRS
LLMTHLQLKQLLTGLMVKNSPEILSRSHLLLAG
QTLIGVVAMVVEAEGEEDPWAVEAMEVVAVVVV
AEEDFPVEVVAVEDSSELVTGSVLIPPVRI*TSL
GGMNATSVRPLNQMAQEGDQVALTWGVTTGMIV
VVAEEAMIEAATGAAAGTVEASEGAGVVGTEVA
LALARWIPGVSTDRIAGRGRIN*PGSPGSGTAFC
PVPSVTLVIL*PSNS*SPKGFFVSDYVIVTIPL
VPIKSDHFS*ILFLFPLFTFLEDRCPDQEGREF
SCSDYPAQQELEYSVRGEGQMISLRAEIKLFCHG
E

Frame 1

GAAQSSRRRYSAVLELRCLLACARVRGHGLKRL
YPTSNPKLWGLPHPARAGLFPAEQSALRTAELQ
WL*PVHGHFRLWPEQLFFLWPEPEHRLWNSVNSP
GIWLDWRLWQ*PELPIVLRAAVLLPWLWPAASS
QQHLGKLR*QFSEQQLWAAPEWELQPAA*LWWT
AAKLWTAAKL*SPSGLWTAEPVQQQQWWWRWRWR
WR*LWPRSILHE*WWWQWWRLWQSRPEWWRWQR
WLWTAGPWRPRQGWQWWRRRRRRWWLQPQQWWL
*TQRSWRWPWRQRWHGRK*PWWLQ*IWWPSGPRI
TS*LRTG*FRQQHHLCARPG*ECYN*VCG*LLQ
ADWYY*DKQENGTAHD*FVHRQGNWQAEGRGNG
LF**PTFS*SSY*LV*W*RILRKSYQGLICYSPG
RL*SGWWQWSWRPRARRTHGPWRLWRWWQWWWW
PRRISQWRWWRWRTAASW*LEVS*SHL*EYELL
LEE*MQPV*GP*TRWPRRGTRWLSHGG*LRG*SS
WWQRRL*SRRLPGPRRGPWRLPRGPGWWGQRWL
WPWQDGFQG*AQTGSQGEAVLISLAPQVLEQLF
VLYPVLPSLFCNLPIPDHPRVFLCRTM*L*LYLW
FPLKVTILVKFCSSSPFSLSWKIDVPIRKVESF
PVQITLPSRNWNTVFGEKAK*YP*EQRLNFSVM
GK

Frame 2

GLLSPPGVGTQRCWNFVACLPVRACADMASNDY
TQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYS
GYSQSTDTSGYGQSSYSSYGQSQNTGYGTQSTP
QGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPAP
SSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQ
QQSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGG
GGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSG
GYGQQDRGGRGRGGSGGGGGGGGGGYNRSSGGY
EPRGRGGGRGGRGGMGGSDRGGFNKFGGPRDQG
SRHDSEQDNSDNNTIFVQGLGENVTIESVADYFK
QIGIIKTNKKTGQPMINLYTDRETGKLKGEATV
SFDDPPSAKAAIDWFDGKEFSGNPIKVSFATRR
ADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGG
RGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFS
WRNECNQCKAPKPDGPGGGPGGSHMGGNYGDDR
RGGRGGYDRGGYRGRGGDRGGFRGGRGGGDRGGF
GPGKMDSRGEHRQDRRERPY*LAWLPRFWNSFL
SCTQCYPRYFVTFQFLITQGFFCVGLCNCNYTS
GSH*K*PF*LNFVPLPPFHFPGRSMSRSGR*RVF
LFRLPCPAGTGIQCSGRRPNDILESRD*TFLSW
G

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