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Sequence NM_012280
GGATACGTCGACAATAGATGACGGGGCTCACGTTGTACGTTCACATCAGGTCCCGGCCCGCCGGAACCTGGGCGATCCACGATGCCGAGTTTGCCACGCTGCGACAGCCCATAGGCTTGCCCCCCCGGGCATTCGGGTGGACTACGAACACAAACTGAAGCCCTAGGACTTGTCGCCCGTTTGCGCTCTCGCCGAGGCAC
AGGCTGCTCGCGGACCACCCTGCTCCGAAAACTAAGAGGTAGGTGGTAGCCCATTCATCTGGTTACTGATACTGGCCGGCATCAGTGGACTGTCGGCAGG
TCCTTGAGCAACTGGTGTGTGAAATGGGACGGACGTCAAAGGACAAGCGGGATGTCTACTACCGCCTGGCCAAGGAGAATGGCTGGCGTGCTCGCAGCGC
CTTCAAACTGCTACAACTGGATAAGGAATTCCAACTCTTCCAAGGCGTGACACGGGCAGTTGACCTGTGTGCAGCCCCAGGCAGCTGGAGCCAGGTGCTG
AGCCAGAAGATCGGGGGCCAAGGGTCCGGCCACGTGGTGGCTGTGGACCTGCAGGCTATGGCTCCACTACCAGGTGTGGTACAGATCCAGGGGGACATCA
CCCAGCTGTCCACTGCCAAGGAGATCATCCAGCACTTTAAGGGCTGCCCTGCGGACCTAGTGGTGTGTGACGGGGCTCCTGATGTAACCGGTCTCCATGA
TGTTGATGAGTATATGCAGGCCCAGCTCCTCCTAGCTGCTCTGAACATTGCTACACATGTCCTGAAGCCAGGGGGCTGCTTTGTGGCCAAGATATTCCGA
GGCCGGGATGTGACGCTCCTCTACAGCCAGCTGCAGGTCTTCTTCTCCAGCGTGCTGTGTGCCAAGCCCAGGAGCAGCCGGAACTCTAGCATCGAGGCCT
TCGCTGTCTGTCAGGGCTATGACCCTCCCGAGGGCTTCATCCCGGACCTGAGCAAACCCCTGCTGGACCATTCTTACGACCCAGATTTCAACCAGCTGGA
TGGTCCCACCCGCATCATTGTGCCTTTTGTGACCTGTGGGGACCTGAGCTCCTATGATTCGGACCGCAGTTACCCACTGGACCTAGAGGGCGGCTCAGAG
TACAAGTACACTCCACCCACACAGCCCCCCATCTCGCCACCATACCAGGAGGCCTGCACGTTGAAGAGGAAGGGGCAGCTGGCCAAGGAGATCCGCCCCC
AGGACTGCCCCATCAGCAGAGTGGACACGTTTCCCCAGCCCCTGGCCGCCCCTCAGTGCCACACCCTGCTGGCCCCTGAGATGGAAGACAATGAAATGAG
TTGTTCACCTTAACCCATTACGCTGGCAAACTGATACAACTCAGAAACTGCTCAATGTCAGGAAAACCGGAACTTGTTGATGAACTTGTTTTCAAAATAT
CATCTCATCAACGAGGCCTGGACAAACAAGCCCTGAGGAGGGATCTGCAACAACCCTGAAGACAACAAGGAAAGAAACCATGAAAGTCTGTCTGTACTGC
AGTGGGAATTCTTGAGTGAGGTCTTACCTCTTCTTTAAACCTCTTCAGGAGTGTCCTGATTATGTCCAGAATTTTCCCTAAAGGCAGGGATTCTTAACCT
GGATAGAAGCCAGGGGTAACCATGAACTTGATGGAAGAAAATGTTACATCTTTATTTTCAGCAATGAAACTGAAATTTAGCCTTACTCCCAAGTTATAAA
TGCTGGCAACAAATCACAGTAGTAAAAGCAGTACCTGGGATTTTCCCACCAATACAAACCAGTTACAGCAACTGTAATGTAACATAAAAAGGGCATCTTG
AAGTATTGTTCATATTTGTCACCTCTTGGAATGATAGCAGATCCTGTAAGTTGATGTATTAATTAAAAAGCCCATATATTACTGCATCTCAATTTTGTTT
CAATTTAATATATAGTAATAATTGTAATTCAATGTAATTGGCTTCCATTGT
- refseq_FTSJ1.F2 (forward primer)
- refseq_FTSJ1.R2 (reverse primer)
- Single exon skipping
Frame 0
GYVDNR*RGSRCTFTSGPGPPEPGRSTMPSLPRCDSP*ACPPGHSGGLRTQTEALGLVARLRSRRGT
GCSRTTLLRKLRGRW*PIHLVTDTGRHQWTVGR
SLSNWCVKWDGRQRTSGMSTTAWPRRMAGVLAA
PSNCYNWIRNSNSSKA*HGQLTCVQPQAAGARC*
ARRSGAKGPATWWLWTCRLWLHYQVWYRSRGTS
PSCPLPRRSSSTLRAALRT*WCVTGLLM*PVSM
MLMSICRPSSS*LL*TLLHMS*SQGAALWPRYSE
AGM*RSSTASCRSSSPACCVPSPGAAGTLASRP
SLSVRAMTLPRASSRT*ANPCWTILTTQISTSW
MVPPASLCLL*PVGT*APMIRTAVTHWT*RAAQS
TSTLHPHSPPSRHHTRRPAR*RGRGSWPRRSAP
RTAPSAEWTRFPSPWPPLSATPCWPLRWKTMK*
VVHLNPLRWQTDTTQKLLNVRKTGTC**TCFQNI
ISSTRPGQTSPEEGSATTLKTTRKETMKVCLYC
SGNS*VRSYLFFKPLQECPDYVQNFP*RQGFLT
WIEARGNHELDGRKCYIFIFSNETEI*PYSQVIN
AGNKSQ**KQYLGFSHQYKPVTATVM*HKKGIL
KYCSYLSPLGMIADPVS*CIN*KAHILLHLNFV
SI*YIVIIVIQCNWLPL
Frame 1
DTSTIDDGAHVVRSHQVPARRNLGDPRCRVCHAATAHRLAPPGIRVDYEHKLKP*DLSPVCALAEA
QAARGPPCSEN*EVGGSPFIWLLILAGISGLSAG
P*ATGV*NGTDVKGQAGCLLPPGQGEWLACSQR
LQTATTG*GIPTLPRRDTGS*PVCSPRQLEPGA
EPEDRGPRVRPRGGCGPAGYGSTTRCGTDPGGHH
PAVHCQGDHPAL*GLPCGPSGV*RGS*CNRSP*
C**VYAGPAPPSCSEHCYTCPEARGLLCGQDIP
RPGCDAPLQPAAGLLLQRAVCQAQEQPEL*HRGL
RCLSGL*PSRGLHPGPEQTPAGPFLRPRFQPAG
WSHPHHCAFCDLWGPELL*FGPQLPTGPRGRLR
VQVHSTHTAPHLATIPGGLHVEEEGAAGQGDPPP
GLPHQQSGHVSPAPGRPSVPHPAGP*DGRQ*NE
LFTLTHYAGKLIQLRNCSMSGKPELVDELVFKI
SSHQRGLDKQALRRDLQQP*RQQGKKP*KSVCTA
VGILE*GLTSSLNLFRSVLIMSRIFPKGRDS*P
G*KPGVTMNLMEENVTSLFSAMKLKFSLTPKL*
MLATNHSSKSSTWDFPTNTNQLQQL*CNIKRAS*
SIVHICHLLE**QIL*VDVLIKKPIYYCISILF
QFNI***L*FNVIGFHC
Frame 2
IRRQ*MTGLTLYVHIRSRPAGTWAIHDAEFATLRQPIGLPPRAFGWTTNTN*SPRTCRPFALSPRH
RLLADHPAPKTKR*VVAHSSGY*YWPASVDCRQ
VLEQLVCEMGRTSKDKRDVYYRLAKENGWRARSA
FKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQVL
SQKIGGQGSGHVVAVDLQAMAPLPGVVQIQGDI
TQLSTAKEIIQHFKGCPADLVVCDGAPDVTGLHD
VDEYMQAQLLLAALNIATHVLKPGGCFVAKIFR
GRDVTLLYSQLQVFFSSVLCAKPRSSRNSSIEA
FAVCQGYDPPEGFIPDLSKPLLDHSYDPDFNQLD
GPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSE
YKYTPPTQPPISPPYQEACTLKRKGQLAKEIRP
QDCPISRVDTFPQPLAAPQCHTLLAPEMEDNEMS
CSP*PITLAN*YNSETAQCQENRNLLMNLFSKY
HLINEAWTNKP*GGICNNPEDNKERNHESLSVL
QWEFLSEVLPLL*TSSGVS*LCPEFSLKAGILNL
DRSQG*P*T*WKKMLHLYFQQ*N*NLALLPSYK
CWQQITVVKAVPGIFPPIQTSYSNCNVT*KGHL
EVLFIFVTSWNDSRSCKLMY*LKSPYITASQFCF
NLIYSNNCNSM*LASI
Sequence NM_177439
GGATACGTCGACAATAGATGACGGGGCTCACGTTGTACGTTCACATCAGGTCCCGGCCCGCCGGAACCTGGGCGATCCACGATGCCGAGTTTGCCACGCTGCGACAGCCCATAGGCTTGCCCCCCCGGGCATTCGGGTGGACTACGAACACAAACTGAAGCCCTAGGACTTGTCGCCCGTTTGCGCTCTCGCCGAGGCAC
AGGCTGCTCGCGGACCACCCTGCTCCGAAAACTAAGAAGGGGACCAGTAGACGGGGTGGATTCGAGGTGGGGACATGGGACGCAGCGGTCGGAGCCGCCT
GGAGGGGTACCCGGTGGTCCCGATGGAGCTATCATCTTTGAGAGGTAGGTGGTAGCCCATTCATCTGGTTACTGATACTGGCCGGCATCAGTGGACTGTC
GGCAGGTCCTTGAGCAACTGGTGTGTGAAATGGGACGGACGTCAAAGGACAAGCGGGATGTCTACTACCGCCTGGCCAAGGAGAATGGCTGGCGTGCTCG
CAGCGCCTTCAAACTGCTACAACTGGATAAGGAATTCCAACTCTTCCAAGGCGTGACACGGGCAGTTGACCTGTGTGCAGCCCCAGGCAGCTGGAGCCAG
GTGCTGAGCCAGAAGATCGGGGGCCAAGGGTCCGGCCACGTGGTGGCTGTGGACCTGCAGGCTATGGCTCCACTACCAGGTGTGGTACAGATCCAGGGGG
ACATCACCCAGCTGTCCACTGCCAAGGAGATCATCCAGCACTTTAAGGGCTGCCCTGCGGACCTAGTGGTGTGTGACGGGGCTCCTGATGTAACCGGTCT
CCATGATGTTGATGAGTATATGCAGGCCCAGCTCCTCCTAGCTGCTCTGAACATTGCTACACATGTCCTGAAGCCAGGGGGCTGCTTTGTGGCCAAGATA
TTCCGAGGCCGGGATGTGACGCTCCTCTACAGCCAGCTGCAGGTCTTCTTCTCCAGCGTGCTGTGTGCCAAGCCCAGGAGCAGCCGGAACTCTAGCATCG
AGGCCTTCGCTGTCTGTCAGGGCTATGACCCTCCCGAGGGCTTCATCCCGGACCTGAGCAAACCCCTGCTGGACCATTCTTACGATTTCAACCAGCTGGA
TGGTCCCACCCGCATCATTGTGCCTTTTGTGACCTGTGGGGACCTGAGCTCCTATGATTCGGACCGCAGTTACCCACTGGACCTAGAGGGCGGCTCAGAG
TACAAGTACACTCCACCCACACAGCCCCCCATCTCGCCACCATACCAGGAGGCCTGCACGTTGAAGAGGAAGGGGCAGCTGGCCAAGGAGATCCGCCCCC
AGGACTGCCCCATCAGCAGAGTGGACACGTTTCCCCAGCCCCTGGCCGCCCCTCAGTGCCACACCCTGCTGGCCCCTGAGATGGAAGACAATGAAATGAG
TTGTTCACCTTAACCCATTACGCTGGCAAACTGATACAACTCAGAAACTGCTCAATGTCAGGAAAACCGGAACTTGTTGATGAACTTGTTTTCAAAATAT
CATCTCATCAACGAGGCCTGGACAAACAAGCCCTGAGGAGGGATCTGCAACAACCCTGAAGACAACAAGGAAAGAAACCATGAAAGTCTGTCTGTACTGC
AGTGGGAATTCTTGAGTGAGGTCTTACCTCTTCTTTAAACCTCTTCAGGAGTGTCCTGATTATGTCCAGAATTTTCCCTAAAGGCAGGGATTCTTAACCT
GGATAGAAGCCAGGGGTAACCATGAACTTGATGGAAGAAAATGTTACATCTTTATTTTCAGCAATGAAACTGAAATTTAGCCTTACTCCCAAGTTATAAA
TGCTGGCAACAAATCACAGTAGTAAAAGCAGTACCTGGGATTTTCCCACCAATACAAACCAGTTACAGCAACTGTAATGTAACATAAAAAGGGCATCTTG
AAGTATTGTTCATATTTGTCACCTCTTGGAATGATAGCAGATCCTGTAAGTTGATGTATTAATTAAAAAGCCCATATATTACTGCATCTCAATTTTGTTT
CAATTTAATATATAGTAATAATTGTAATTCAATGTAATTGGCTTCCATTGT
- refseq_FTSJ1.F2 (forward primer)
- refseq_FTSJ1.R2 (reverse primer)
- Single exon skipping
Frame 0
GYVDNR*RGSRCTFTSGPGPPEPGRSTMPSLPRCDSP*ACPPGHSGGLRTQTEALGLVARLRSRRGT
GCSRTTLLRKLRRGPVDGVDSRWGHGTQRSEPP
GGVPGGPDGAIIFER*VVAHSSGY*YWPASVDC
RQVLEQLVCEMGRTSKDKRDVYYRLAKENGWRAR
SAFKLLQLDKEFQLFQGVTRAVDLCAAPGSWSQ
VLSQKIGGQGSGHVVAVDLQAMAPLPGVVQIQG
DITQLSTAKEIIQHFKGCPADLVVCDGAPDVTGL
HDVDEYMQAQLLLAALNIATHVLKPGGCFVAKI
FRGRDVTLLYSQLQVFFSSVLCAKPRSSRNSSI
EAFAVCQGYDPPEGFIPDLSKPLLDHSYDFNQLD
GPTRIIVPFVTCGDLSSYDSDRSYPLDLEGGSE
YKYTPPTQPPISPPYQEACTLKRKGQLAKEIRP
QDCPISRVDTFPQPLAAPQCHTLLAPEMEDNEMS
CSP*PITLAN*YNSETAQCQENRNLLMNLFSKY
HLINEAWTNKP*GGICNNPEDNKERNHESLSVL
QWEFLSEVLPLL*TSSGVS*LCPEFSLKAGILNL
DRSQG*P*T*WKKMLHLYFQQ*N*NLALLPSYK
CWQQITVVKAVPGIFPPIQTSYSNCNVT*KGHL
EVLFIFVTSWNDSRSCKLMY*LKSPYITASQFCF
NLIYSNNCNSM*LASI
Frame 1
DTSTIDDGAHVVRSHQVPARRNLGDPRCRVCHAATAHRLAPPGIRVDYEHKLKP*DLSPVCALAEA
QAARGPPCSEN*EGDQ*TGWIRGGDMGRSGRSRL
EGYPVVPMELSSLRGRW*PIHLVTDTGRHQWTV
GRSLSNWCVKWDGRQRTSGMSTTAWPRRMAGVL
AAPSNCYNWIRNSNSSKA*HGQLTCVQPQAAGAR
C*ARRSGAKGPATWWLWTCRLWLHYQVWYRSRG
TSPSCPLPRRSSSTLRAALRT*WCVTGLLM*PV
SMMLMSICRPSSS*LL*TLLHMS*SQGAALWPRY
SEAGM*RSSTASCRSSSPACCVPSPGAAGTLAS
RPSLSVRAMTLPRASSRT*ANPCWTILTISTSW
MVPPASLCLL*PVGT*APMIRTAVTHWT*RAAQS
TSTLHPHSPPSRHHTRRPAR*RGRGSWPRRSAP
RTAPSAEWTRFPSPWPPLSATPCWPLRWKTMK*
VVHLNPLRWQTDTTQKLLNVRKTGTC**TCFQNI
ISSTRPGQTSPEEGSATTLKTTRKETMKVCLYC
SGNS*VRSYLFFKPLQECPDYVQNFP*RQGFLT
WIEARGNHELDGRKCYIFIFSNETEI*PYSQVIN
AGNKSQ**KQYLGFSHQYKPVTATVM*HKKGIL
KYCSYLSPLGMIADPVS*CIN*KAHILLHLNFV
SI*YIVIIVIQCNWLPL
Frame 2
IRRQ*MTGLTLYVHIRSRPAGTWAIHDAEFATLRQPIGLPPRAFGWTTNTN*SPRTCRPFALSPRH
RLLADHPAPKTKKGTSRRGGFEVGTWDAAVGAA
WRGTRWSRWSYHL*EVGGSPFIWLLILAGISGLS
AGP*ATGV*NGTDVKGQAGCLLPPGQGEWLACS
QRLQTATTG*GIPTLPRRDTGS*PVCSPRQLEP
GAEPEDRGPRVRPRGGCGPAGYGSTTRCGTDPGG
HHPAVHCQGDHPAL*GLPCGPSGV*RGS*CNRS
P*C**VYAGPAPPSCSEHCYTCPEARGLLCGQD
IPRPGCDAPLQPAAGLLLQRAVCQAQEQPEL*HR
GLRCLSGL*PSRGLHPGPEQTPAGPFLRFQPAG
WSHPHHCAFCDLWGPELL*FGPQLPTGPRGRLR
VQVHSTHTAPHLATIPGGLHVEEEGAAGQGDPPP
GLPHQQSGHVSPAPGRPSVPHPAGP*DGRQ*NE
LFTLTHYAGKLIQLRNCSMSGKPELVDELVFKI
SSHQRGLDKQALRRDLQQP*RQQGKKP*KSVCTA
VGILE*GLTSSLNLFRSVLIMSRIFPKGRDS*P
G*KPGVTMNLMEENVTSLFSAMKLKFSLTPKL*
MLATNHSSKSSTWDFPTNTNQLQQL*CNIKRAS*
SIVHICHLLE**QIL*VDVLIKKPIYYCISILF
QFNI***L*FNVIGFHC
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