Sequence NM_207047
GGTTGGAAAGACGGTGAATGGAAAGGGGATGGCTGGTGGTCTTGGGTGTGATGTGTGTTATTGGTTTGTAGAGGACACGCAGGAGAAAGAAGGTATTCTG
CCTGAGAGAGCTGAAGAGGCAAAGCTAAAGGCCAAATACCCAAGCCTAGGACAAAAGCCTGGAGGCTCCGACTTCCTCATGAAGAGACTCCAGAAAGGGC
AAAAGTACTTTGACTCAGGAGACTACAACATGGCCAAAGCCAAGATGAAGAATAAGCAGCTGCCAAGTGCAGGACCAGACAAGAACCTGGTGACTGGTGA
TCACATCCCCACCCCACAGGATCTGCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGGGGTAACCTGAGCCCCCCTCTCCTCCCCTTCCTCAA
CCACTGGACGTTTATATATTATAGGCAGGGATGAAATGGGCACCTAGTCAGATCTTCTCAGCTTGCTAGCCAGAAATGACTGTGATTCTGCTGGGGGCTG
CTGAGAAGGTAATGTAGGTTGAAAAGGGGCTCTAAGTTTATTTATTTCGTTAGATTGACACTTCCACACACTCCCTGTAGTCCAGGTAGGGCCCAGAAAT
AGGAAAGGCTAGGATTGGATAATGCTGCAAATGCTTTTTTTGTGTGAGAAACTGGAGAGATGTGATTTCTCCTTTTGGGAGAGAATGTCCCAAAATTGAT
TAGGCTGAGCCTTGGGAATAGTTTGGCAGGTTTAACATCCCAAGGCTAACCTAACGTAGTTGGGAAAGGTAGATTGAATGAGACATGTTTTCTGTGCTTC
TAAGTGTTCTGTCCCTTAGGCTGCTATTGCTTCATGTTTCCATTATGGCAGGTTTAGAGAATCCTTAAAAAGAAAAATTGACTTGCTTGCCTAAAACTAC
AGTGCCCCCTTAGCCTCCATTACTTAGTATCTCTTACAGTTTGCTCTGGCTCTCAAATAATATAAAGATTGATGAACATTATTCACAGAAAATGGGCACC
TTCTCTCTCTCTTCCAGCATGTTGCTTTTGAAAGTATCATGGGATAGTGGGACGAGCACTGGTTTAGGAGTCAAGATATCTGGGTTCTCACTCTACTCAG
TCCCAGCAGAGCTGTTGTATAACCTTGATTAAGTCATTTAGCCTCTCTGGATTTCTATTTCCTCATCTGTAAAATAGAGTTAAATGTATGTAAGATTGAT
TCTGATTCTGAAGTCTTAACTGCCAGCAGAAAAACTCCATACTGTTCATTGTAAAACTAAAAGTGAGGAAGGCTCGTGGGTGGTGAACCTCTGCTCTGTA
ATACTGGGAAAGTACTACAGAGGGGAACCATTTGAGGGATACATGAGGAGAGAGTAAATTGAGGTTTGGGGATTATAAATTCAGGCAAGAGAACCTTATA
GATATCACAGTCTTGAGGGTCAAAAAAAAATACTTAGGAAGCTAGCCATGGAAGCTTCTTGCCTCTGACCCAGCCCACTTTCCCAGCCTACCTCTGGGCC
TTAGCTGCTAAAAAGCTTCTCTGGCAGCGGAGCTGCAGGCCTGAGGAAACATGCTCAGTCATGCACATGTGTTGACCCATGTTTCAGATGCAGTCTGATG
CCAGGTATATTGTTAGGGAAAGAGGAAGAAGGGTAAACTGAGCTAGCCCCTGGGGCTGAGTACTCCTGCAGGGCTGGAGGAACAGGTTCTTAGAAGATGA
TTCACCTTGGAGGAAGCTGGGGAAAGTGGTTAGGGAGGGAGAGGAGGAAGACTGAGAAATCTCTGCATCCCCAGAACTCAGCAACCCAGCTCTTTTATCA
TGAGGAGAGGTAGCCATGCTGACTCATGAACAGGTGGAGGAAAGGGGTTGCAGGTGACCAGCATCTTCAATCTAGTTACCACCAGCTTGGCTAGAATTTT
ATTGAACGCAACTTGCTAGTTATGACAGAGAGGACCAGGGTGAGAACTAATGCTCAAGAGAGCAAGATTCTGTAATCCTCTTGCCCTCTCACATCACAAA
TTCTGATATGCCAAACATTTGGCCCAATGAGAATGACTGATAGACTTGCTGATGTGCATGTGGGGGGATGAGGTGTGTATATGCCTTTCTCCTGACTGGC
CATTCAGTGTTCAGGCTGTCATTCAAATTGGATAGGAGAAAGTTGGTGAGGAAGGAATGGAGAGAGCAGGATCCCTGGACTCCCTGGTCCCCAAACTCCT
TGACTGTCACTTGTAATTGTATATAATTTTTGTGTTTCTTGCTCCATTTCCTCATGCTGTCATCCTTAAAGTCCATCTGGGAAGGGGAAAATGCTGAACA
CCATTGTATAGTTTCTTCAACTGTCCCAGCCATGTTGTACATAGATATGTCATGTTATTATATATATAAATATATATATAATTTTGTACGTTTTCTTCAT
CTCCGATCTTCTCAAATTCTGTACTTTTTTTCTTTTCTGTCTGAGCTGCCTTCTCTATCATTCAGCTTGAGTCCTCAAGACTACTGGTCACAATGAGTCT
GATTTTTAGGAAGGAATAGGAGGAGCAGGGAAATCAGAAAAACTGTTGGTCTCACTCTGTCTCTCTACCTGGTCATCTTCAAGGCTAAGAAAACAAACAA
GCTACATTCTGTATTTGCCAGTTTCCACAGCATGAAGTACATAGCTTCCTTAGCAAGGAGTGGTCATTCCAAAATCCAAATGCAAATTGCTTTGATTAAA
CCTAGATTATTAAATCCATTCAGAACAGCA
- refseq_ENSA.F1 (forward primer)
- refseq_ENSA.R1 (reverse primer)
- Single exon skipping
Frame 0
GWKDGEWKGDGWWSWV*CVLLVCRGHAGERRYS
A*ES*RGKAKGQIPKPRTKAWRLRLPHEETPERA
KVL*LRRLQHGQSQDEE*AAAKCRTRQEPGDW*
SHPHPTGSAPEKVLARHQQACGVT*APLSSPSS
TTGRLYIIGRDEMGT*SDLLSLLARNDCDSAGGC
*EGNVG*KGALSLFISLD*HFHTLPVVQVGPRN
RKG*DWIMLQMLFLCEKLERCDFSFWERMSQN*
LG*ALGIVWQV*HPKANLT*LGKVD*MRHVFCAS
KCSVP*AAIASCFHYGRFRESLKRKIDLLA*NY
SAPLASIT*YLLQFALALK*YKD**TLFTENGH
LLSLFQHVAFESIMG*WDEHWFRSQDIWVLTLLS
PSRAVV*P*LSHLASLDFYFLICKIELNVCKID
SDSEVLTASRKTPYCSL*N*K*GRLVGGEPLLC
NTGKVLQRGTI*GIHEERVN*GLGIINSGKRTL*
ISQS*GSKKNT*EASHGSFLPLTQPTFPAYLWA
LAAKKLLWQRSCRPEETCSVMHMC*PMFQMQSD
ARYIVRERGRRVN*ASPWG*VLLQGWRNRFLEDD
SPWRKLGKVVREGEEED*EISASPELSNPALLS
*GEVAMLTHEQVEERGCR*PASSI*LPPAWLEF
Y*TQLASYDREDQGEN*CSREQDSVILLPSHITN
SDMPNIWPNEND**TC*CACGGMRCVYAFLLTG
HSVFRLSFKLDRRKLVRKEWREQDPWTPWSPNS
LTVTCNCI*FLCFLLHFLMLSSLKSIWEGENAEH
HCIVSSTVPAMLYIDMSCYYIYKYIYNFVRFLH
LRSSQILYFFSFLSELPSLSFSLSPQDYWSQ*V
*FLGRNRRSREIRKTVGLTLSLYLVIFKAKKTNK
LHSVFASFHSMKYIASLARSGHSKIQMQIALIK
PRLLNPFRTA
Frame 1
VGKTVNGKGMAGGLGCDVCYWFVEDTQEKEGIL
PERAEEAKLKAKYPSLGQKPGGSDFLMKRLQKG
QKYFDSGDYNMAKAKMKNKQLPSAGPDKNLVTGD
HIPTPQDLPQRKSSLVTSKLAG*PEPPSPPLPQ
PLDVYIL*AGMKWAPSQIFSAC*PEMTVILLGA
AEKVM*VEKGL*VYLFR*IDTSTHSL*SR*GPEI
GKARIG*CCKCFFCVRNWRDVISPFGRECPKID
*AEPWE*FGRFNIPRLT*RSWER*IE*DMFSVL
LSVLSLRLLLLHVSIMAGLENP*KEKLTCLPKTT
VPP*PPLLSISYSLLWLSNNIKIDEHYSQKMGT
FSLSSSMLLLKVSWDSGTSTGLGVKISGFSLYS
VPAELLYNLD*VI*PLWISISSSVK*S*MYVRLI
LILKS*LPAEKLHTVHCKTKSEEGSWVVNLCSV
ILGKYYRGEPFEGYMRRE*IEVWGL*IQAREPY
RYHSLEGQKKILRKLAMEASCL*PSPLSQPTSGP
*LLKSFSGSGAAGLRKHAQSCTCVDPCFRCSLM
PGILLGKEEEG*TELAPGAEYSCRAGGTGS*KM
IHLGGSWGKWLGRERRKTEKSLHPQNSATQLFYH
EER*PC*LMNRWRKGVAGDQHLQSSYHQLG*NF
IERNLLVMTERTRVRTNAQESKIL*SSCPLTSQ
ILICQTFGPMRMTDRLADVHVGG*GVYMPFS*LA
IQCSGCHSNWIGESW*GRNGESRIPGLPGPQTP
*LSLVIVYNFCVSCSISSCCHP*SPSGKGKMLN
TIV*FLQLSQPCCT*ICHVIIYINIYIILYVFFI
SDLLKFCTFFLFCLSCLLYHSA*VLKTTGHNES
DF*EGIGGAGKSEKLLVSLCLSTWSSSRLRKQT
SYILYLPVSTA*ST*LP*QGVVIPKSKCKLL*LN
LDY*IHSEQ
Frame 2
LERR*MERGWLVVLGVMCVIGL*RTRRRKKVFC
LRELKRQS*RPNTQA*DKSLEAPTSS*RDSRKG
KSTLTQETTTWPKPR*RISSCQVQDQTRTW*LV
ITSPPHRICPRESPRSSPASLRGNLSPPLLPFLN
HWTFIYYRQG*NGHLVRSSQLASQK*L*FCWGL
LRR*CRLKRGSKFIYFVRLTLPHTPCSPGRAQK
*ERLGLDNAANAFFV*ETGEM*FLLLGENVPKLI
RLSLGNSLAGLTSQG*PNVVGKGRLNETCFLCF
*VFCPLGCYCFMFPLWQV*RILKKKN*LACLKL
QCPLSLHYLVSLTVCSGSQII*RLMNIIHRKWAP
SLSLPACCF*KYHGIVGRALV*ESRYLGSHSTQ
SQQSCCITLIKSFSLSGFLFPHL*NRVKCM*D*
F*F*SLNCQQKNSILFIVKLKVRKARGW*TSAL*
YWESTTEGNHLRDT*GESKLRFGDYKFRQENLI
DITVLRVKKKYLGS*PWKLLASDPAHFPSLPLG
LSC*KASLAAELQA*GNMLSHAHVLTHVSDAV*C
QVYC*GKRKKGKLS*PLGLSTPAGLEEQVLRR*
FTLEEAGESG*GGRGGRLRNLCIPRTQQPSSFI
MRRGSHADS*TGGGKGLQVTSIFNLVTTSLARIL
LNATC*L*QRGPG*ELMLKRARFCNPLALSHHK
F*YAKHLAQ*E*LIDLLMCMWGDEVCICLSPDW
PFSVQAVIQIG*EKVGEEGMERAGSLDSLVPKLL
DCHL*LYIIFVFLAPFPHAVILKVHLGRGKC*T
PLYSFFNCPSHVVHRYVMLLYI*IYI*FCTFSS
SPIFSNSVLFFFSV*AAFSIIQLESSRLLVTMSL
IFRKE*EEQGNQKNCWSHSVSLPGHLQG*ENKQ
ATFCICQFPQHEVHSFLSKEWSFQNPNANCFD*
T*IIKSIQNS
Sequence NM_207046
GGTTGGAAAGACGGTGAATGGAAAGGGGATGGCTGGTGGTCTTGGGTGTGATGTGTGTTATTGGTTTGTAGAGGACACGCAGGAGAAAGAAGGTATTCTG
CCTGAGAGAGCTGAAGAGGCAAAGCTAAAGGCCAAATACCCAAGCCTAGGACAAAAGCCTGGAGGCTCCGACTTCCTCATGAAGAGACTCCAGAAAGGGC
AAAAGTACTTTGACTCAGGAGACTACAACATGGCCAAAGCCAAGATGAAGAATAAGCAGCTGCCAAGTGCAGGACCAGACAAGAACCTGGTGACTGGTGA
TCACATCCCCACCCCACAGGATCTGCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGGGTGGCCAAGTTGAATGATGCTGCCCGGGGCTCTGC
CAGATCCTGAGACGCTTCCCCTCCCTGCCCCACCCGGGTCCTGTGCTGGCTCCTGCCCCTTCCTGCTTTTGCAGCCAGGGGTCAGGAGGTGGCTCGGGTG
TGGGCTGGAGAGGCAGAAGCCCTTTCCTGTTGGTGTCCCAGCACATGGAGCCCCTTGGGCTGAGCACCAAGACCTTGAACCTTTTTTGTTTTACCTTTTT
TCCAAATAACAGTTGGGAGAAATATCAATGAAATTCTGGGGGTGGGGGTGGGGTTGAAAGGGTGGGGTGGGAGATATGGAGGAGTATGAATTAGGGCTTG
GAGTTGGTAAAAACATTCCTGACTATCCTTCTTAACCACGTGGCTGATGTGGGGTAGGTATGAGGGGAAGGAAGTGGAGTAGCCTAATGAAAAGGGGTTC
TAGTTGAGCTCTGTAGATAAATGCCTTGTTTCAGTGTGGTTGGAGACCTGGTGTCAGATAAAAGAAACTCCATCCGCACAGACAGATGCAAACAGCTCCT
CTAGTTCTGCAGAGCTAGTTGAGAACTCAACATTAATCATTTTAAAAAGTACTGTCCTTGAAATAGATTTGCTGTGGGAAGAAGGGCAGTGAGTGTGGGA
GAAAGGAGCCGTGAGCGTGGGGAACCCCACAGAGCCCAAAGGACTTTTTCAGTATTCGAAATAAACAAAACAAAAACCCATGAAAAAACCCAACCCAGAA
ATA
- refseq_ENSA.F1 (forward primer)
- refseq_ENSA.R1 (reverse primer)
- Single exon skipping
Frame 0
GWKDGEWKGDGWWSWV*CVLLVCRGHAGERRYS
A*ES*RGKAKGQIPKPRTKAWRLRLPHEETPERA
KVL*LRRLQHGQSQDEE*AAAKCRTRQEPGDW*
SHPHPTGSAPEKVLARHQQACGWPS*MMLPGAL
PDPETLPLPAPPGSCAGSCPFLLLQPGVRRWLGC
GLERQKPFPVGVPAHGAPWAEHQDLEPFLFYLF
SK*QLGEISMKFWGWGWG*KGGVGDMEEYELGL
GVGKNIPDYPS*PRG*CGVGMRGRKWSSLMKRGS
S*AL*INALFQCGWRPGVR*KKLHPHRQMQTAP
LVLQS*LRTQH*SF*KVLSLK*ICCGKKGSECG
RKEP*AWGTPQSPKDFFSIRNKQNKNP*KNPTQK
Frame 1
VGKTVNGKGMAGGLGCDVCYWFVEDTQEKEGIL
PERAEEAKLKAKYPSLGQKPGGSDFLMKRLQKG
QKYFDSGDYNMAKAKMKNKQLPSAGPDKNLVTGD
HIPTPQDLPQRKSSLVTSKLAGGQVE*CCPGLC
QILRRFPSLPHPGPVLAPAPSCFCSQGSGGGSG
VGWRGRSPFLLVSQHMEPLGLSTKTLNLFCFTFF
PNNSWEKYQ*NSGGGGGVERVGWEIWRSMN*GL
ELVKTFLTILLNHVADVG*V*GEGSGVA**KGV
LVELCR*MPCFSVVGDLVSDKRNSIRTDRCKQLL
*FCRAS*ELNINHFKKYCP*NRFAVGRRAVSVG
ERSRERGEPHRAQRTFSVFEINKTKTHEKTQPR
N
Frame 2
LERR*MERGWLVVLGVMCVIGL*RTRRRKKVFC
LRELKRQS*RPNTQA*DKSLEAPTSS*RDSRKG
KSTLTQETTTWPKPR*RISSCQVQDQTRTW*LV
ITSPPHRICPRESPRSSPASLRVAKLNDAARGSA
RS*DASPPCPTRVLCWLLPLPAFAARGQEVARV
WAGEAEALSCWCPSTWSPLG*APRP*TFFVLPF
FQITVGRNINEILGVGVGLKGWGGRYGGV*IRAW
SW*KHS*LSFLTTWLMWGRYEGKEVE*PNEKGF
*LSSVDKCLVSVWLETWCQIKETPSAQTDANSS
SSSAELVENSTLIILKSTVLEIDLLWEEGQ*VWE
KGAVSVGNPTEPKGLFQYSK*TKQKPMKKPNPE
I
Sequence NM_004436
TCGCAACGTCACGGGCAAGGGCCGCCATTTTGACTGAGCAACCCTAGTGACAGGAGCCGAAGCAGCAGCGCAGGTTGTCCCCGTTTCCCCTCCCCCTTCC
CTTCTCCGGTTGCCTTCCCGGGCCCCTTACACTCCACAGTCCCGGTCCCGCCATGTCCCAGAAACAAGAAGAAGAGAACCCTGCGGAGGAGACCGGCGAG
GAGAAGCAGGACACGCAGGAGAAAGAAGGTATTCTGCCTGAGAGAGCTGAAGAGGCAAAGCTAAAGGCCAAATACCCAAGCCTAGGACAAAAGCCTGGAG
GCTCCGACTTCCTCATGAAGAGACTCCAGAAAGGGCAAAAGTACTTTGACTCAGGAGACTACAACATGGCCAAAGCCAAGATGAAGAATAAGCAGCTGCC
AAGTGCAGGACCAGACAAGAACCTGGTGACTGGTGATCACATCCCCACCCCACAGGATCTGCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCG
GGTGGCCAAGTTGAATGATGCTGCCCGGGGCTCTGCCAGATCCTGAGACGCTTCCCCTCCCTGCCCCACCCGGGTCCTGTGCTGGCTCCTGCCCCTTCCT
GCTTTTGCAGCCAGGGGTCAGGAGGTGGCTCGGGTGTGGGCTGGAGAGGCAGAAGCCCTTTCCTGTTGGTGTCCCAGCACATGGAGCCCCTTGGGCTGAG
CACCAAGACCTTGAACCTTTTTTGTTTTACCTTTTTTCCAAATAACAGTTGGGAGAAATATCAATGAAATTCTGGGGGTGGGGGTGGGGTTGAAAGGGTG
GGGTGGGAGATATGGAGGAGTATGAATTAGGGCTTGGAGTTGGTAAAAACATTCCTGACTATCCTTCTTAACCACGTGGCTGATGTGGGGTAGGTATGAG
GGGAAGGAAGTGGAGTAGCCTAATGAAAAGGGGTTCTAGTTGAGCTCTGTAGATAAATGCCTTGTTTCAGTGTGGTTGGAGACCTGGTGTCAGATAAAAG
AAACTCCATCCGCACAGACAGATGCAAACAGCTCCTCTAGTTCTGCAGAGCTAGTTGAGAACTCAACATTAATCATTTTAAAAAGTACTGTCCTTGAAAT
AGATTTGCTGTGGGAAGAAGGGCAGTGAGTGTGGGAGAAAGGAGCCGTGAGCGTGGGGAACCCCACAGAGCCCAAAGGACTTTTTCAGTATTCGAAATAA
ACAAAACAAAAACCCATGAAAAAACCCAACCCAGAAATA
- refseq_ENSA.F1 (forward primer)
- refseq_ENSA.R1 (reverse primer)
- Single exon skipping
Frame 0
SQRHGQGPPF*LSNPSDRSRSSSAGCPRFPSPF
PSPVAFPGPLHSTVPVPPCPRNKKKRTLRRRPAR
RSRTRRRKKVFCLRELKRQS*RPNTQA*DKSLE
APTSS*RDSRKGKSTLTQETTTWPKPR*RISSC
QVQDQTRTW*LVITSPPHRICPRESPRSSPASLR
VAKLNDAARGSARS*DASPPCPTRVLCWLLPLP
AFAARGQEVARVWAGEAEALSCWCPSTWSPLG*
APRP*TFFVLPFFQITVGRNINEILGVGVGLKGW
GGRYGGV*IRAWSW*KHS*LSFLTTWLMWGRYE
GKEVE*PNEKGF*LSSVDKCLVSVWLETWCQIK
ETPSAQTDANSSSSSAELVENSTLIILKSTVLEI
DLLWEEGQ*VWEKGAVSVGNPTEPKGLFQYSK*
TKQKPMKKPNPEI
Frame 1
RNVTGKGRHFD*ATLVTGAEAAAQVVPVSPPPS
LLRLPSRAPYTPQSRSRHVPETRRREPCGGDRR
GEAGHAGERRYSA*ES*RGKAKGQIPKPRTKAWR
LRLPHEETPERAKVL*LRRLQHGQSQDEE*AAA
KCRTRQEPGDW*SHPHPTGSAPEKVLARHQQAC
GWPS*MMLPGALPDPETLPLPAPPGSCAGSCPFL
LLQPGVRRWLGCGLERQKPFPVGVPAHGAPWAE
HQDLEPFLFYLFSK*QLGEISMKFWGWGWG*KG
GVGDMEEYELGLGVGKNIPDYPS*PRG*CGVGMR
GRKWSSLMKRGSS*AL*INALFQCGWRPGVR*K
KLHPHRQMQTAPLVLQS*LRTQH*SF*KVLSLK
*ICCGKKGSECGRKEP*AWGTPQSPKDFFSIRNK
QNKNP*KNPTQK
Frame 2
ATSRARAAILTEQP**QEPKQQRRLSPFPLPLP
FSGCLPGPLTLHSPGPAMSQKQEEENPAEETGE
EKQDTQEKEGILPERAEEAKLKAKYPSLGQKPG
GSDFLMKRLQKGQKYFDSGDYNMAKAKMKNKQLP
SAGPDKNLVTGDHIPTPQDLPQRKSSLVTSKLA
GGQVE*CCPGLCQILRRFPSLPHPGPVLAPAPS
CFCSQGSGGGSGVGWRGRSPFLLVSQHMEPLGLS
TKTLNLFCFTFFPNNSWEKYQ*NSGGGGGVERV
GWEIWRSMN*GLELVKTFLTILLNHVADVG*V*
GEGSGVA**KGVLVELCR*MPCFSVVGDLVSDKR
NSIRTDRCKQLL*FCRAS*ELNINHFKKYCP*N
RFAVGRRAVSVGERSRERGEPHRAQRTFSVFEI
NKTKTHEKTQPRN
Sequence NM_207044
TCGCAACGTCACGGGCAAGGGCCGCCATTTTGACTGAGCAACCCTAGTGACAGGAGCCGAAGCAGCAGCGCAGGTTGTCCCCGTTTCCCCTCCCCCTTCC
CTTCTCCGGTTGCCTTCCCGGGCCCCTTACACTCCACAGTCCCGGTCCCGCCATGTCCCAGAAACAAGAAGAAGAGAACCCTGCGGAGGAGACCGGCGAG
GAGAAGCAGGACACGCAGGAGAAAGAAGGTATTCTGCCTGAGAGAGCTGAAGAGGCAAAGCTAAAGGCCAAATACCCAAGCCTAGGACAAAAGCCTGGAG
GCTCCGACTTCCTCATGAAGAGACTCCAGAAAGGGCAAAAGTACTTTGACTCAGGAGACTACAACATGGCCAAAGCCAAGATGAAGAATAAGCAGCTGCC
AAGTGCAGGACCAGACAAGAACCTGGTGACTGGTGATCACATCCCCACCCCACAGGATCTGCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCG
GGGTAACCTGAGCCCCCCTCTCCTCCCCTTCCTCAACCACTGGACGTTTATATATTATAGGCAGGGATGAAATGGGCACCTAGTCAGATCTTCTCAGCTT
GCTAGCCAGAAATGACTGTGATTCTGCTGGGGGCTGCTGAGAAGGTAATGTAGGTTGAAAAGGGGCTCTAAGTTTATTTATTTCGTTAGATTGACACTTC
CACACACTCCCTGTAGTCCAGGTAGGGCCCAGAAATAGGAAAGGCTAGGATTGGATAATGCTGCAAATGCTTTTTTTGTGTGAGAAACTGGAGAGATGTG
ATTTCTCCTTTTGGGAGAGAATGTCCCAAAATTGATTAGGCTGAGCCTTGGGAATAGTTTGGCAGGTTTAACATCCCAAGGCTAACCTAACGTAGTTGGG
AAAGGTAGATTGAATGAGACATGTTTTCTGTGCTTCTAAGTGTTCTGTCCCTTAGGCTGCTATTGCTTCATGTTTCCATTATGGCAGGTTTAGAGAATCC
TTAAAAAGAAAAATTGACTTGCTTGCCTAAAACTACAGTGCCCCCTTAGCCTCCATTACTTAGTATCTCTTACAGTTTGCTCTGGCTCTCAAATAATATA
AAGATTGATGAACATTATTCACAGAAAATGGGCACCTTCTCTCTCTCTTCCAGCATGTTGCTTTTGAAAGTATCATGGGATAGTGGGACGAGCACTGGTT
TAGGAGTCAAGATATCTGGGTTCTCACTCTACTCAGTCCCAGCAGAGCTGTTGTATAACCTTGATTAAGTCATTTAGCCTCTCTGGATTTCTATTTCCTC
ATCTGTAAAATAGAGTTAAATGTATGTAAGATTGATTCTGATTCTGAAGTCTTAACTGCCAGCAGAAAAACTCCATACTGTTCATTGTAAAACTAAAAGT
GAGGAAGGCTCGTGGGTGGTGAACCTCTGCTCTGTAATACTGGGAAAGTACTACAGAGGGGAACCATTTGAGGGATACATGAGGAGAGAGTAAATTGAGG
TTTGGGGATTATAAATTCAGGCAAGAGAACCTTATAGATATCACAGTCTTGAGGGTCAAAAAAAAATACTTAGGAAGCTAGCCATGGAAGCTTCTTGCCT
CTGACCCAGCCCACTTTCCCAGCCTACCTCTGGGCCTTAGCTGCTAAAAAGCTTCTCTGGCAGCGGAGCTGCAGGCCTGAGGAAACATGCTCAGTCATGC
ACATGTGTTGACCCATGTTTCAGATGCAGTCTGATGCCAGGTATATTGTTAGGGAAAGAGGAAGAAGGGTAAACTGAGCTAGCCCCTGGGGCTGAGTACT
CCTGCAGGGCTGGAGGAACAGGTTCTTAGAAGATGATTCACCTTGGAGGAAGCTGGGGAAAGTGGTTAGGGAGGGAGAGGAGGAAGACTGAGAAATCTCT
GCATCCCCAGAACTCAGCAACCCAGCTCTTTTATCATGAGGAGAGGTAGCCATGCTGACTCATGAACAGGTGGAGGAAAGGGGTTGCAGGTGACCAGCAT
CTTCAATCTAGTTACCACCAGCTTGGCTAGAATTTTATTGAACGCAACTTGCTAGTTATGACAGAGAGGACCAGGGTGAGAACTAATGCTCAAGAGAGCA
AGATTCTGTAATCCTCTTGCCCTCTCACATCACAAATTCTGATATGCCAAACATTTGGCCCAATGAGAATGACTGATAGACTTGCTGATGTGCATGTGGG
GGGATGAGGTGTGTATATGCCTTTCTCCTGACTGGCCATTCAGTGTTCAGGCTGTCATTCAAATTGGATAGGAGAAAGTTGGTGAGGAAGGAATGGAGAG
AGCAGGATCCCTGGACTCCCTGGTCCCCAAACTCCTTGACTGTCACTTGTAATTGTATATAATTTTTGTGTTTCTTGCTCCATTTCCTCATGCTGTCATC
CTTAAAGTCCATCTGGGAAGGGGAAAATGCTGAACACCATTGTATAGTTTCTTCAACTGTCCCAGCCATGTTGTACATAGATATGTCATGTTATTATATA
TATAAATATATATATAATTTTGTACGTTTTCTTCATCTCCGATCTTCTCAAATTCTGTACTTTTTTTCTTTTCTGTCTGAGCTGCCTTCTCTATCATTCA
GCTTGAGTCCTCAAGACTACTGGTCACAATGAGTCTGATTTTTAGGAAGGAATAGGAGGAGCAGGGAAATCAGAAAAACTGTTGGTCTCACTCTGTCTCT
CTACCTGGTCATCTTCAAGGCTAAGAAAACAAACAAGCTACATTCTGTATTTGCCAGTTTCCACAGCATGAAGTACATAGCTTCCTTAGCAAGGAGTGGT
CATTCCAAAATCCAAATGCAAATTGCTTTGATTAAACCTAGATTATTAAATCCATTCAGAACAGCA
- refseq_ENSA.F1 (forward primer)
- refseq_ENSA.R1 (reverse primer)
- Single exon skipping
Frame 0
SQRHGQGPPF*LSNPSDRSRSSSAGCPRFPSPF
PSPVAFPGPLHSTVPVPPCPRNKKKRTLRRRPAR
RSRTRRRKKVFCLRELKRQS*RPNTQA*DKSLE
APTSS*RDSRKGKSTLTQETTTWPKPR*RISSC
QVQDQTRTW*LVITSPPHRICPRESPRSSPASLR
GNLSPPLLPFLNHWTFIYYRQG*NGHLVRSSQL
ASQK*L*FCWGLLRR*CRLKRGSKFIYFVRLTL
PHTPCSPGRAQK*ERLGLDNAANAFFV*ETGEM*
FLLLGENVPKLIRLSLGNSLAGLTSQG*PNVVG
KGRLNETCFLCF*VFCPLGCYCFMFPLWQV*RI
LKKKN*LACLKLQCPLSLHYLVSLTVCSGSQII*
RLMNIIHRKWAPSLSLPACCF*KYHGIVGRALV
*ESRYLGSHSTQSQQSCCITLIKSFSLSGFLFP
HL*NRVKCM*D*F*F*SLNCQQKNSILFIVKLKV
RKARGW*TSAL*YWESTTEGNHLRDT*GESKLR
FGDYKFRQENLIDITVLRVKKKYLGS*PWKLLA
SDPAHFPSLPLGLSC*KASLAAELQA*GNMLSHA
HVLTHVSDAV*CQVYC*GKRKKGKLS*PLGLST
PAGLEEQVLRR*FTLEEAGESG*GGRGGRLRNL
CIPRTQQPSSFIMRRGSHADS*TGGGKGLQVTSI
FNLVTTSLARILLNATC*L*QRGPG*ELMLKRA
RFCNPLALSHHKF*YAKHLAQ*E*LIDLLMCMW
GDEVCICLSPDWPFSVQAVIQIG*EKVGEEGMER
AGSLDSLVPKLLDCHL*LYIIFVFLAPFPHAVI
LKVHLGRGKC*TPLYSFFNCPSHVVHRYVMLLY
I*IYI*FCTFSSSPIFSNSVLFFFSV*AAFSIIQ
LESSRLLVTMSLIFRKE*EEQGNQKNCWSHSVS
LPGHLQG*ENKQATFCICQFPQHEVHSFLSKEW
SFQNPNANCFD*T*IIKSIQNS
Frame 1
RNVTGKGRHFD*ATLVTGAEAAAQVVPVSPPPS
LLRLPSRAPYTPQSRSRHVPETRRREPCGGDRR
GEAGHAGERRYSA*ES*RGKAKGQIPKPRTKAWR
LRLPHEETPERAKVL*LRRLQHGQSQDEE*AAA
KCRTRQEPGDW*SHPHPTGSAPEKVLARHQQAC
GVT*APLSSPSSTTGRLYIIGRDEMGT*SDLLSL
LARNDCDSAGGC*EGNVG*KGALSLFISLD*HF
HTLPVVQVGPRNRKG*DWIMLQMLFLCEKLERC
DFSFWERMSQN*LG*ALGIVWQV*HPKANLT*LG
KVD*MRHVFCASKCSVP*AAIASCFHYGRFRES
LKRKIDLLA*NYSAPLASIT*YLLQFALALK*Y
KD**TLFTENGHLLSLFQHVAFESIMG*WDEHWF
RSQDIWVLTLLSPSRAVV*P*LSHLASLDFYFL
ICKIELNVCKIDSDSEVLTASRKTPYCSL*N*K
*GRLVGGEPLLCNTGKVLQRGTI*GIHEERVN*G
LGIINSGKRTL*ISQS*GSKKNT*EASHGSFLP
LTQPTFPAYLWALAAKKLLWQRSCRPEETCSVM
HMC*PMFQMQSDARYIVRERGRRVN*ASPWG*VL
LQGWRNRFLEDDSPWRKLGKVVREGEEED*EIS
ASPELSNPALLS*GEVAMLTHEQVEERGCR*PA
SSI*LPPAWLEFY*TQLASYDREDQGEN*CSREQ
DSVILLPSHITNSDMPNIWPNEND**TC*CACG
GMRCVYAFLLTGHSVFRLSFKLDRRKLVRKEWR
EQDPWTPWSPNSLTVTCNCI*FLCFLLHFLMLSS
LKSIWEGENAEHHCIVSSTVPAMLYIDMSCYYI
YKYIYNFVRFLHLRSSQILYFFSFLSELPSLSF
SLSPQDYWSQ*V*FLGRNRRSREIRKTVGLTLSL
YLVIFKAKKTNKLHSVFASFHSMKYIASLARSG
HSKIQMQIALIKPRLLNPFRTA
Frame 2
ATSRARAAILTEQP**QEPKQQRRLSPFPLPLP
FSGCLPGPLTLHSPGPAMSQKQEEENPAEETGE
EKQDTQEKEGILPERAEEAKLKAKYPSLGQKPG
GSDFLMKRLQKGQKYFDSGDYNMAKAKMKNKQLP
SAGPDKNLVTGDHIPTPQDLPQRKSSLVTSKLA
G*PEPPSPPLPQPLDVYIL*AGMKWAPSQIFSA
C*PEMTVILLGAAEKVM*VEKGL*VYLFR*IDTS
THSL*SR*GPEIGKARIG*CCKCFFCVRNWRDV
ISPFGRECPKID*AEPWE*FGRFNIPRLT*RSW
ER*IE*DMFSVLLSVLSLRLLLLHVSIMAGLENP
*KEKLTCLPKTTVPP*PPLLSISYSLLWLSNNI
KIDEHYSQKMGTFSLSSSMLLLKVSWDSGTSTG
LGVKISGFSLYSVPAELLYNLD*VI*PLWISISS
SVK*S*MYVRLILILKS*LPAEKLHTVHCKTKS
EEGSWVVNLCSVILGKYYRGEPFEGYMRRE*IE
VWGL*IQAREPYRYHSLEGQKKILRKLAMEASCL
*PSPLSQPTSGP*LLKSFSGSGAAGLRKHAQSC
TCVDPCFRCSLMPGILLGKEEEG*TELAPGAEY
SCRAGGTGS*KMIHLGGSWGKWLGRERRKTEKSL
HPQNSATQLFYHEER*PC*LMNRWRKGVAGDQH
LQSSYHQLG*NFIERNLLVMTERTRVRTNAQES
KIL*SSCPLTSQILICQTFGPMRMTDRLADVHVG
G*GVYMPFS*LAIQCSGCHSNWIGESW*GRNGE
SRIPGLPGPQTP*LSLVIVYNFCVSCSISSCCH
P*SPSGKGKMLNTIV*FLQLSQPCCT*ICHVIIY
INIYIILYVFFISDLLKFCTFFLFCLSCLLYHS
A*VLKTTGHNESDF*EGIGGAGKSEKLLVSLCL
STWSSSRLRKQTSYILYLPVSTA*ST*LP*QGVV
IPKSKCKLL*LNLDY*IHSEQ
Sequence NM_207043
TCGCAACGTCACGGGCAAGGGCCGCCATTTTGACTGAGCAACCCTAGTGACAGGAGCCGAAGCAGCAGCGCAGGTTGTCCCCGTTTCCCCTCCCCCTTCC
CTTCTCCGGTTGCCTTCCCGGGCCCCTTACACTCCACAGTCCCGGTCCCGCCATGTCCCAGAAACAAGAAGAAGAGAACCCTGCGGAGGAGACCGGCGAG
GAGAAGCAGGACACGCAGGAGAAAGAAGGTATTCTGCCTGAGAGAGCTGAAGAGGCAAAGCTAAAGGCCAAATACCCAAGCCTAGGACAAAAGCCTGGAG
GCTCCGACTTCCTCATGAAGAGACTCCAGAAAGGGGATTATAAATCATTACATTGGAGTGTGCTTCTCTGTGCGGATGAAATGCAAAAGTACTTTGACTC
AGGAGACTACAACATGGCCAAAGCCAAGATGAAGAATAAGCAGCTGCCAAGTGCAGGACCAGACAAGAACCTGGTGACTGGTGATCACATCCCCACCCCA
CAGGATCTGCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGGGGTAACCTGAGCCCCCCTCTCCTCCCCTTCCTCAACCACTGGACGTTTATA
TATTATAGGCAGGGATGAAATGGGCACCTAGTCAGATCTTCTCAGCTTGCTAGCCAGAAATGACTGTGATTCTGCTGGGGGCTGCTGAGAAGGTAATGTA
GGTTGAAAAGGGGCTCTAAGTTTATTTATTTCGTTAGATTGACACTTCCACACACTCCCTGTAGTCCAGGTAGGGCCCAGAAATAGGAAAGGCTAGGATT
GGATAATGCTGCAAATGCTTTTTTTGTGTGAGAAACTGGAGAGATGTGATTTCTCCTTTTGGGAGAGAATGTCCCAAAATTGATTAGGCTGAGCCTTGGG
AATAGTTTGGCAGGTTTAACATCCCAAGGCTAACCTAACGTAGTTGGGAAAGGTAGATTGAATGAGACATGTTTTCTGTGCTTCTAAGTGTTCTGTCCCT
TAGGCTGCTATTGCTTCATGTTTCCATTATGGCAGGTTTAGAGAATCCTTAAAAAGAAAAATTGACTTGCTTGCCTAAAACTACAGTGCCCCCTTAGCCT
CCATTACTTAGTATCTCTTACAGTTTGCTCTGGCTCTCAAATAATATAAAGATTGATGAACATTATTCACAGAAAATGGGCACCTTCTCTCTCTCTTCCA
GCATGTTGCTTTTGAAAGTATCATGGGATAGTGGGACGAGCACTGGTTTAGGAGTCAAGATATCTGGGTTCTCACTCTACTCAGTCCCAGCAGAGCTGTT
GTATAACCTTGATTAAGTCATTTAGCCTCTCTGGATTTCTATTTCCTCATCTGTAAAATAGAGTTAAATGTATGTAAGATTGATTCTGATTCTGAAGTCT
TAACTGCCAGCAGAAAAACTCCATACTGTTCATTGTAAAACTAAAAGTGAGGAAGGCTCGTGGGTGGTGAACCTCTGCTCTGTAATACTGGGAAAGTACT
ACAGAGGGGAACCATTTGAGGGATACATGAGGAGAGAGTAAATTGAGGTTTGGGGATTATAAATTCAGGCAAGAGAACCTTATAGATATCACAGTCTTGA
GGGTCAAAAAAAAATACTTAGGAAGCTAGCCATGGAAGCTTCTTGCCTCTGACCCAGCCCACTTTCCCAGCCTACCTCTGGGCCTTAGCTGCTAAAAAGC
TTCTCTGGCAGCGGAGCTGCAGGCCTGAGGAAACATGCTCAGTCATGCACATGTGTTGACCCATGTTTCAGATGCAGTCTGATGCCAGGTATATTGTTAG
GGAAAGAGGAAGAAGGGTAAACTGAGCTAGCCCCTGGGGCTGAGTACTCCTGCAGGGCTGGAGGAACAGGTTCTTAGAAGATGATTCACCTTGGAGGAAG
CTGGGGAAAGTGGTTAGGGAGGGAGAGGAGGAAGACTGAGAAATCTCTGCATCCCCAGAACTCAGCAACCCAGCTCTTTTATCATGAGGAGAGGTAGCCA
TGCTGACTCATGAACAGGTGGAGGAAAGGGGTTGCAGGTGACCAGCATCTTCAATCTAGTTACCACCAGCTTGGCTAGAATTTTATTGAACGCAACTTGC
TAGTTATGACAGAGAGGACCAGGGTGAGAACTAATGCTCAAGAGAGCAAGATTCTGTAATCCTCTTGCCCTCTCACATCACAAATTCTGATATGCCAAAC
ATTTGGCCCAATGAGAATGACTGATAGACTTGCTGATGTGCATGTGGGGGGATGAGGTGTGTATATGCCTTTCTCCTGACTGGCCATTCAGTGTTCAGGC
TGTCATTCAAATTGGATAGGAGAAAGTTGGTGAGGAAGGAATGGAGAGAGCAGGATCCCTGGACTCCCTGGTCCCCAAACTCCTTGACTGTCACTTGTAA
TTGTATATAATTTTTGTGTTTCTTGCTCCATTTCCTCATGCTGTCATCCTTAAAGTCCATCTGGGAAGGGGAAAATGCTGAACACCATTGTATAGTTTCT
TCAACTGTCCCAGCCATGTTGTACATAGATATGTCATGTTATTATATATATAAATATATATATAATTTTGTACGTTTTCTTCATCTCCGATCTTCTCAAA
TTCTGTACTTTTTTTCTTTTCTGTCTGAGCTGCCTTCTCTATCATTCAGCTTGAGTCCTCAAGACTACTGGTCACAATGAGTCTGATTTTTAGGAAGGAA
TAGGAGGAGCAGGGAAATCAGAAAAACTGTTGGTCTCACTCTGTCTCTCTACCTGGTCATCTTCAAGGCTAAGAAAACAAACAAGCTACATTCTGTATTT
GCCAGTTTCCACAGCATGAAGTACATAGCTTCCTTAGCAAGGAGTGGTCATTCCAAAATCCAAATGCAAATTGCTTTGATTAAACCTAGATTATTAAATC
CATTCAGAACAGCA
- refseq_ENSA.F1 (forward primer)
- refseq_ENSA.R1 (reverse primer)
- Single exon skipping
Frame 0
SQRHGQGPPF*LSNPSDRSRSSSAGCPRFPSPF
PSPVAFPGPLHSTVPVPPCPRNKKKRTLRRRPAR
RSRTRRRKKVFCLRELKRQS*RPNTQA*DKSLE
APTSS*RDSRKGIINHYIGVCFSVRMKCKSTLT
QETTTWPKPR*RISSCQVQDQTRTW*LVITSPPH
RICPRESPRSSPASLRGNLSPPLLPFLNHWTFI
YYRQG*NGHLVRSSQLASQK*L*FCWGLLRR*C
RLKRGSKFIYFVRLTLPHTPCSPGRAQK*ERLGL
DNAANAFFV*ETGEM*FLLLGENVPKLIRLSLG
NSLAGLTSQG*PNVVGKGRLNETCFLCF*VFCP
LGCYCFMFPLWQV*RILKKKN*LACLKLQCPLSL
HYLVSLTVCSGSQII*RLMNIIHRKWAPSLSLP
ACCF*KYHGIVGRALV*ESRYLGSHSTQSQQSC
CITLIKSFSLSGFLFPHL*NRVKCM*D*F*F*SL
NCQQKNSILFIVKLKVRKARGW*TSAL*YWEST
TEGNHLRDT*GESKLRFGDYKFRQENLIDITVL
RVKKKYLGS*PWKLLASDPAHFPSLPLGLSC*KA
SLAAELQA*GNMLSHAHVLTHVSDAV*CQVYC*
GKRKKGKLS*PLGLSTPAGLEEQVLRR*FTLEE
AGESG*GGRGGRLRNLCIPRTQQPSSFIMRRGSH
ADS*TGGGKGLQVTSIFNLVTTSLARILLNATC
*L*QRGPG*ELMLKRARFCNPLALSHHKF*YAK
HLAQ*E*LIDLLMCMWGDEVCICLSPDWPFSVQA
VIQIG*EKVGEEGMERAGSLDSLVPKLLDCHL*
LYIIFVFLAPFPHAVILKVHLGRGKC*TPLYSF
FNCPSHVVHRYVMLLYI*IYI*FCTFSSSPIFSN
SVLFFFSV*AAFSIIQLESSRLLVTMSLIFRKE
*EEQGNQKNCWSHSVSLPGHLQG*ENKQATFCI
CQFPQHEVHSFLSKEWSFQNPNANCFD*T*IIKS
IQNS
Frame 1
RNVTGKGRHFD*ATLVTGAEAAAQVVPVSPPPS
LLRLPSRAPYTPQSRSRHVPETRRREPCGGDRR
GEAGHAGERRYSA*ES*RGKAKGQIPKPRTKAWR
LRLPHEETPERGL*IITLECASLCG*NAKVL*L
RRLQHGQSQDEE*AAAKCRTRQEPGDW*SHPHP
TGSAPEKVLARHQQACGVT*APLSSPSSTTGRLY
IIGRDEMGT*SDLLSLLARNDCDSAGGC*EGNV
G*KGALSLFISLD*HFHTLPVVQVGPRNRKG*D
WIMLQMLFLCEKLERCDFSFWERMSQN*LG*ALG
IVWQV*HPKANLT*LGKVD*MRHVFCASKCSVP
*AAIASCFHYGRFRESLKRKIDLLA*NYSAPLA
SIT*YLLQFALALK*YKD**TLFTENGHLLSLFQ
HVAFESIMG*WDEHWFRSQDIWVLTLLSPSRAV
V*P*LSHLASLDFYFLICKIELNVCKIDSDSEV
LTASRKTPYCSL*N*K*GRLVGGEPLLCNTGKVL
QRGTI*GIHEERVN*GLGIINSGKRTL*ISQS*
GSKKNT*EASHGSFLPLTQPTFPAYLWALAAKK
LLWQRSCRPEETCSVMHMC*PMFQMQSDARYIVR
ERGRRVN*ASPWG*VLLQGWRNRFLEDDSPWRK
LGKVVREGEEED*EISASPELSNPALLS*GEVA
MLTHEQVEERGCR*PASSI*LPPAWLEFY*TQLA
SYDREDQGEN*CSREQDSVILLPSHITNSDMPN
IWPNEND**TC*CACGGMRCVYAFLLTGHSVFR
LSFKLDRRKLVRKEWREQDPWTPWSPNSLTVTCN
CI*FLCFLLHFLMLSSLKSIWEGENAEHHCIVS
STVPAMLYIDMSCYYIYKYIYNFVRFLHLRSSQ
ILYFFSFLSELPSLSFSLSPQDYWSQ*V*FLGRN
RRSREIRKTVGLTLSLYLVIFKAKKTNKLHSVF
ASFHSMKYIASLARSGHSKIQMQIALIKPRLLN
PFRTA
Frame 2
ATSRARAAILTEQP**QEPKQQRRLSPFPLPLP
FSGCLPGPLTLHSPGPAMSQKQEEENPAEETGE
EKQDTQEKEGILPERAEEAKLKAKYPSLGQKPG
GSDFLMKRLQKGDYKSLHWSVLLCADEMQKYFDS
GDYNMAKAKMKNKQLPSAGPDKNLVTGDHIPTP
QDLPQRKSSLVTSKLAG*PEPPSPPLPQPLDVY
IL*AGMKWAPSQIFSAC*PEMTVILLGAAEKVM*
VEKGL*VYLFR*IDTSTHSL*SR*GPEIGKARI
G*CCKCFFCVRNWRDVISPFGRECPKID*AEPW
E*FGRFNIPRLT*RSWER*IE*DMFSVLLSVLSL
RLLLLHVSIMAGLENP*KEKLTCLPKTTVPP*P
PLLSISYSLLWLSNNIKIDEHYSQKMGTFSLSS
SMLLLKVSWDSGTSTGLGVKISGFSLYSVPAELL
YNLD*VI*PLWISISSSVK*S*MYVRLILILKS
*LPAEKLHTVHCKTKSEEGSWVVNLCSVILGKY
YRGEPFEGYMRRE*IEVWGL*IQAREPYRYHSLE
GQKKILRKLAMEASCL*PSPLSQPTSGP*LLKS
FSGSGAAGLRKHAQSCTCVDPCFRCSLMPGILL
GKEEEG*TELAPGAEYSCRAGGTGS*KMIHLGGS
WGKWLGRERRKTEKSLHPQNSATQLFYHEER*P
C*LMNRWRKGVAGDQHLQSSYHQLG*NFIERNL
LVMTERTRVRTNAQESKIL*SSCPLTSQILICQT
FGPMRMTDRLADVHVGG*GVYMPFS*LAIQCSG
CHSNWIGESW*GRNGESRIPGLPGPQTP*LSLV
IVYNFCVSCSISSCCHP*SPSGKGKMLNTIV*FL
QLSQPCCT*ICHVIIYINIYIILYVFFISDLLK
FCTFFLFCLSCLLYHSA*VLKTTGHNESDF*EG
IGGAGKSEKLLVSLCLSTWSSSRLRKQTSYILYL
PVSTA*ST*LP*QGVVIPKSKCKLL*LNLDY*I
HSEQ
Sequence NM_207042
TCGCAACGTCACGGGCAAGGGCCGCCATTTTGACTGAGCAACCCTAGTGACAGGAGCCGAAGCAGCAGCGCAGGTTGTCCCCGTTTCCCCTCCCCCTTCC
CTTCTCCGGTTGCCTTCCCGGGCCCCTTACACTCCACAGTCCCGGTCCCGCCATGTCCCAGAAACAAGAAGAAGAGAACCCTGCGGAGGAGACCGGCGAG
GAGAAGCAGGACACGCAGGAGAAAGAAGGTATTCTGCCTGAGAGAGCTGAAGAGGCAAAGCTAAAGGCCAAATACCCAAGCCTAGGACAAAAGCCTGGAG
GCTCCGACTTCCTCATGAAGAGACTCCAGAAAGGGGATTATAAATCATTACATTGGAGTGTGCTTCTCTGTGCGGATGAAATGCAAAAGTACTTTGACTC
AGGAGACTACAACATGGCCAAAGCCAAGATGAAGAATAAGCAGCTGCCAAGTGCAGGACCAGACAAGAACCTGGTGACTGGTGATCACATCCCCACCCCA
CAGGATCTGCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGGGTGGCCAAGTTGAATGATGCTGCCCGGGGCTCTGCCAGATCCTGAGACGCT
TCCCCTCCCTGCCCCACCCGGGTCCTGTGCTGGCTCCTGCCCCTTCCTGCTTTTGCAGCCAGGGGTCAGGAGGTGGCTCGGGTGTGGGCTGGAGAGGCAG
AAGCCCTTTCCTGTTGGTGTCCCAGCACATGGAGCCCCTTGGGCTGAGCACCAAGACCTTGAACCTTTTTTGTTTTACCTTTTTTCCAAATAACAGTTGG
GAGAAATATCAATGAAATTCTGGGGGTGGGGGTGGGGTTGAAAGGGTGGGGTGGGAGATATGGAGGAGTATGAATTAGGGCTTGGAGTTGGTAAAAACAT
TCCTGACTATCCTTCTTAACCACGTGGCTGATGTGGGGTAGGTATGAGGGGAAGGAAGTGGAGTAGCCTAATGAAAAGGGGTTCTAGTTGAGCTCTGTAG
ATAAATGCCTTGTTTCAGTGTGGTTGGAGACCTGGTGTCAGATAAAAGAAACTCCATCCGCACAGACAGATGCAAACAGCTCCTCTAGTTCTGCAGAGCT
AGTTGAGAACTCAACATTAATCATTTTAAAAAGTACTGTCCTTGAAATAGATTTGCTGTGGGAAGAAGGGCAGTGAGTGTGGGAGAAAGGAGCCGTGAGC
GTGGGGAACCCCACAGAGCCCAAAGGACTTTTTCAGTATTCGAAATAAACAAAACAAAAACCCATGAAAAAACCCAACCCAGAAATA
- refseq_ENSA.F1 (forward primer)
- refseq_ENSA.R1 (reverse primer)
- Single exon skipping
Frame 0
SQRHGQGPPF*LSNPSDRSRSSSAGCPRFPSPF
PSPVAFPGPLHSTVPVPPCPRNKKKRTLRRRPAR
RSRTRRRKKVFCLRELKRQS*RPNTQA*DKSLE
APTSS*RDSRKGIINHYIGVCFSVRMKCKSTLT
QETTTWPKPR*RISSCQVQDQTRTW*LVITSPPH
RICPRESPRSSPASLRVAKLNDAARGSARS*DA
SPPCPTRVLCWLLPLPAFAARGQEVARVWAGEA
EALSCWCPSTWSPLG*APRP*TFFVLPFFQITVG
RNINEILGVGVGLKGWGGRYGGV*IRAWSW*KH
S*LSFLTTWLMWGRYEGKEVE*PNEKGF*LSSV
DKCLVSVWLETWCQIKETPSAQTDANSSSSSAEL
VENSTLIILKSTVLEIDLLWEEGQ*VWEKGAVS
VGNPTEPKGLFQYSK*TKQKPMKKPNPEI
Frame 1
RNVTGKGRHFD*ATLVTGAEAAAQVVPVSPPPS
LLRLPSRAPYTPQSRSRHVPETRRREPCGGDRR
GEAGHAGERRYSA*ES*RGKAKGQIPKPRTKAWR
LRLPHEETPERGL*IITLECASLCG*NAKVL*L
RRLQHGQSQDEE*AAAKCRTRQEPGDW*SHPHP
TGSAPEKVLARHQQACGWPS*MMLPGALPDPETL
PLPAPPGSCAGSCPFLLLQPGVRRWLGCGLERQ
KPFPVGVPAHGAPWAEHQDLEPFLFYLFSK*QL
GEISMKFWGWGWG*KGGVGDMEEYELGLGVGKNI
PDYPS*PRG*CGVGMRGRKWSSLMKRGSS*AL*
INALFQCGWRPGVR*KKLHPHRQMQTAPLVLQS
*LRTQH*SF*KVLSLK*ICCGKKGSECGRKEP*A
WGTPQSPKDFFSIRNKQNKNP*KNPTQK
Frame 2
ATSRARAAILTEQP**QEPKQQRRLSPFPLPLP
FSGCLPGPLTLHSPGPAMSQKQEEENPAEETGE
EKQDTQEKEGILPERAEEAKLKAKYPSLGQKPG
GSDFLMKRLQKGDYKSLHWSVLLCADEMQKYFDS
GDYNMAKAKMKNKQLPSAGPDKNLVTGDHIPTP
QDLPQRKSSLVTSKLAGGQVE*CCPGLCQILRR
FPSLPHPGPVLAPAPSCFCSQGSGGGSGVGWRGR
SPFLLVSQHMEPLGLSTKTLNLFCFTFFPNNSW
EKYQ*NSGGGGGVERVGWEIWRSMN*GLELVKT
FLTILLNHVADVG*V*GEGSGVA**KGVLVELCR
*MPCFSVVGDLVSDKRNSIRTDRCKQLL*FCRA
S*ELNINHFKKYCP*NRFAVGRRAVSVGERSRE
RGEPHRAQRTFSVFEINKTKTHEKTQPRN
Sequence NM_207045
GGTTGGAAAGACGGTGAATGGAAAGGGGATGGCTGGTGGTCTTGGGTGTGATGTGTGTTATTGGTTTGTAGAGGACACGCAGGAGAAAGAAGGTATTCTG
CCTGAGAGAGCTGAAGAGGCAAAGCTAAAGGCCAAATACCCAAGCCTAGGACAAAAGCCTGGAGGCTCCGACTTCCTCATGAAGAGACTCCAGAAAGGGG
ATTATAAATCATTACATTGGAGTGTGCTTCTCTGTGCGGATGAAATGCAAAAGTACTTTGACTCAGGAGACTACAACATGGCCAAAGCCAAGATGAAGAA
TAAGCAGCTGCCAAGTGCAGGACCAGACAAGAACCTGGTGACTGGTGATCACATCCCCACCCCACAGGATCTGCCCCAGAGAAAGTCCTCGCTCGTCACC
AGCAAGCTTGCGGGTGGCCAAGTTGAATGATGCTGCCCGGGGCTCTGCCAGATCCTGAGACGCTTCCCCTCCCTGCCCCACCCGGGTCCTGTGCTGGCTC
CTGCCCCTTCCTGCTTTTGCAGCCAGGGGTCAGGAGGTGGCTCGGGTGTGGGCTGGAGAGGCAGAAGCCCTTTCCTGTTGGTGTCCCAGCACATGGAGCC
CCTTGGGCTGAGCACCAAGACCTTGAACCTTTTTTGTTTTACCTTTTTTCCAAATAACAGTTGGGAGAAATATCAATGAAATTCTGGGGGTGGGGGTGGG
GTTGAAAGGGTGGGGTGGGAGATATGGAGGAGTATGAATTAGGGCTTGGAGTTGGTAAAAACATTCCTGACTATCCTTCTTAACCACGTGGCTGATGTGG
GGTAGGTATGAGGGGAAGGAAGTGGAGTAGCCTAATGAAAAGGGGTTCTAGTTGAGCTCTGTAGATAAATGCCTTGTTTCAGTGTGGTTGGAGACCTGGT
GTCAGATAAAAGAAACTCCATCCGCACAGACAGATGCAAACAGCTCCTCTAGTTCTGCAGAGCTAGTTGAGAACTCAACATTAATCATTTTAAAAAGTAC
TGTCCTTGAAATAGATTTGCTGTGGGAAGAAGGGCAGTGAGTGTGGGAGAAAGGAGCCGTGAGCGTGGGGAACCCCACAGAGCCCAAAGGACTTTTTCAG
TATTCGAAATAAACAAAACAAAAACCCATGAAAAAACCCAACCCAGAAATA
- refseq_ENSA.F1 (forward primer)
- refseq_ENSA.R1 (reverse primer)
- Single exon skipping
Frame 0
GWKDGEWKGDGWWSWV*CVLLVCRGHAGERRYS
A*ES*RGKAKGQIPKPRTKAWRLRLPHEETPERG
L*IITLECASLCG*NAKVL*LRRLQHGQSQDEE
*AAAKCRTRQEPGDW*SHPHPTGSAPEKVLARH
QQACGWPS*MMLPGALPDPETLPLPAPPGSCAGS
CPFLLLQPGVRRWLGCGLERQKPFPVGVPAHGA
PWAEHQDLEPFLFYLFSK*QLGEISMKFWGWGW
G*KGGVGDMEEYELGLGVGKNIPDYPS*PRG*CG
VGMRGRKWSSLMKRGSS*AL*INALFQCGWRPG
VR*KKLHPHRQMQTAPLVLQS*LRTQH*SF*KV
LSLK*ICCGKKGSECGRKEP*AWGTPQSPKDFFS
IRNKQNKNP*KNPTQK
Frame 1
VGKTVNGKGMAGGLGCDVCYWFVEDTQEKEGIL
PERAEEAKLKAKYPSLGQKPGGSDFLMKRLQKG
DYKSLHWSVLLCADEMQKYFDSGDYNMAKAKMKN
KQLPSAGPDKNLVTGDHIPTPQDLPQRKSSLVT
SKLAGGQVE*CCPGLCQILRRFPSLPHPGPVLA
PAPSCFCSQGSGGGSGVGWRGRSPFLLVSQHMEP
LGLSTKTLNLFCFTFFPNNSWEKYQ*NSGGGGG
VERVGWEIWRSMN*GLELVKTFLTILLNHVADV
G*V*GEGSGVA**KGVLVELCR*MPCFSVVGDLV
SDKRNSIRTDRCKQLL*FCRAS*ELNINHFKKY
CP*NRFAVGRRAVSVGERSRERGEPHRAQRTFS
VFEINKTKTHEKTQPRN
Frame 2
LERR*MERGWLVVLGVMCVIGL*RTRRRKKVFC
LRELKRQS*RPNTQA*DKSLEAPTSS*RDSRKG
IINHYIGVCFSVRMKCKSTLTQETTTWPKPR*R
ISSCQVQDQTRTW*LVITSPPHRICPRESPRSSP
ASLRVAKLNDAARGSARS*DASPPCPTRVLCWL
LPLPAFAARGQEVARVWAGEAEALSCWCPSTWS
PLG*APRP*TFFVLPFFQITVGRNINEILGVGVG
LKGWGGRYGGV*IRAWSW*KHS*LSFLTTWLMW
GRYEGKEVE*PNEKGF*LSSVDKCLVSVWLETW
CQIKETPSAQTDANSSSSSAELVENSTLIILKST
VLEIDLLWEEGQ*VWEKGAVSVGNPTEPKGLFQ
YSK*TKQKPMKKPNPEI