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Sequence NM_198174
AGGAGATGTGCCAAACTGTTAAGAGTGGTTATTTCTGAGCAGAAGAATGTGGATGAATTCCATTCTTCCTATTTTTCTGGTCCCAAGGAGAAGCGGATATTGTCCTCCAGCACTGGGGGCAGGAATGACCAAGGAAAGAGGTACTACCATGGCATGGAATATGAGACGGACCTCACTCCCCTTGAAAGCCCCACACACCT
CATGAAATTCCTGACAGAGAACGTGTCTGGAACCCCAGAGTACCCAGATTTGCTCAAGAAGAATAACCTGATGAGCTTGGAGGGGGCCTTGCCCACCCCT
GGCAAGGCAGCTCCCCTCCCTGCAGGCCCCAGCAAGCTGGAGGCCGGCTCTGTGGACAGCTACCTGTTACCCACCACTGATATGTATGATAATGGCTCCC
TCAACTCCTTGTTTGAGAGCATTCATGGGGTGCCGCCCACACAGCGCTGGCAGCCAGACAGCACCTTCAAAGATGACCCACAGGAGTCGATGCTCTTCCC
AGATATCCTGAAAACCTCCCCGGAACCCCCATGTCCAGAGGACTACCCCAGCCTCAAAAGTGACTTTGAATACACCCTGGGCTCCCCCAAAGCCATCCAC
ATCAAGTCAGGCGAGTCACCCATGGCCTACCTCAACAAAGGCCAGTTCTACCCCGTCACCCTGCGGACCCCAGCAGGTGGCAAAGGCCTTGCCTTGTCCT
CCAACAAAGTCAAGAGTGTGGTGATGGTTGTCTTCGACAATGAGAAGGTCCCAGTAGAGCAGCTGCGCTTCTGGAAGCACTGGCATTCCCGGCAACCCAC
TGCCAAGCAGCGGGTCATTGACGTGGCTGACTGCAAAGAAAACTTCAACACTGTGGAGCACATTGAGGAGGTGGCCTATAATGCACTGTCCTTTGTGTGG
AACGTGAATGAAGAGGCCAAGGTGTTCATCGGCGTAAACTGTCTGAGCACAGACTTTTCCTCACAAAAGGGGGTGAAGGGTGTCCCCCTGAACCTGCAGA
TTGACACCTATGACTGTGGCTTGGGCACTGAGCGCCTGGTACACCGTGCTGTCTGCCAGATCAAGATCTTCTGTGACAAGGGAGCTGAGAGGAAGATGCG
CGATGACGAGCGGAAGCAGTTCCGGAGGAAGGTCAAGTGCCCTGACTCCAGCAACAGTGGCGTCAAGGGCTGCCTGCTGTCGGGCTTCAGGGGCAATGAG
ACGACCTACCTTCGGCCAGAGACTGACCTGGAGACGCCACCCGTGCTGTTCATCCCCAATGTGCACTTCTCCAGCCTGCAGCGCTCTGGAGGGGCAGCCC
CCTCGGCAGGACCCAGCAGCTCCAACAGGCTGCCTCTGAAGCGTACCTGCTCGCCCTTCACTGAGGAGTTTGAGCCTCTGCCCTCCAAGCAGGCCAAGGA
AGGCGACCTTCAGAGAGTTCTGCTGTATGTGCGGAGGGAGACTGAGGAGGTGTTTGACGCGCTCATGTTGAAGACCCCAGACCTGAAGGGGCTGAGGAAT
GCGATCTCTGAGAAGTATGGGTTCCCTGAAGAGAACATTTACAAAGTCTACAAGAAATGCAAGCGAGGAATCTTAGTCAACATGGACAACAACATCATTC
AGCATTACAGCAACCACGTCGCCTTCCTGCTGGACATGGGGGAGCTGGACGGCAAAATTCAGATCATCCTTAAGGAGCTGTAAGGCCTCTCGAGCATCCA
AACCCTCACGACCTGCAAGGGGCCAGCAGGGACGTGGCCCCACGCCACACACAACCTCTCCACATGCCTCAGCGCTGTTACTTGAATGCCTTCCCTGAGG
GAAGAGGCCCTTGAGTCACAGACCCACAGACGTCAGGGCCAGGGAGAGACCTAGGGGGTCCCCTGGCCTGGATCCCCATGGTATGCTTGAATCTGCTCCC
TGAACTTCCTGCCAGTGCCTCCCCGTACCCCAAAACAATGTCACCATGGTTACCACCTACCCAGAAGACTGTTCCCTCCTCCCAAGACCCTTGTCTGCAG
TGGTGCTCCTGCAGGCTGCCCGTTAAGATGGTGGCGGCACACGCTCCCTCCCGCAGCACCACGCCAGCTGGTGCGGCCCCCACTCTCTGTCTTCCTTCAA
CTTCAGACAAAGGATTTCTCAACCTTTGGTCAGTTAACTTGAAAACTCTTGATTTTCAGTGCAAATGACTTTTAAAAGACACTATATTGGAGTCTCTTTC
TCAGACTTCCTCAGCGCAGGATGTAAATAGCACTAACGATCGACTGGAACAAAGTGACCGCTGTGTAAAACTACTGCCTTGCCACTCACTGTTGTATACA
TTTCTTATTTACGATTTTCATTTGTTATATATATATATAAATATACTGTATATATATGCAACATTTTATATTTTTCATGGATATGTTTTTATCATTTCAA
AAAATGTGTATTTCACATTTCTTGGACTTTTTTTAGCTGTTATTCAGTGATGCATTTTGTATACTCACGTGGTATTTAGTAATAAAAATCTATCTATGTA
TTACGTCACATTA
- refseq_GRHL3.F1 (forward primer)
- refseq_GRHL3.R1 (reverse primer)
- Single exon skipping
Frame 0
RRCAKLLRVVISEQKNVDEFHSSYFSGPKEKRILSSSTGGRNDQGKRYYHGMEYETDLTPLESPTHL
MKFLTENVSGTPEYPDLLKKNNLMSLEGALPTP
GKAAPLPAGPSKLEAGSVDSYLLPTTDMYDNGS
LNSLFESIHGVPPTQRWQPDSTFKDDPQESMLFP
DILKTSPEPPCPEDYPSLKSDFEYTLGSPKAIH
IKSGESPMAYLNKGQFYPVTLRTPAGGKGLALS
SNKVKSVVMVVFDNEKVPVEQLRFWKHWHSRQPT
AKQRVIDVADCKENFNTVEHIEEVAYNALSFVW
NVNEEAKVFIGVNCLSTDFSSQKGVKGVPLNLQ
IDTYDCGLGTERLVHRAVCQIKIFCDKGAERKMR
DDERKQFRRKVKCPDSSNSGVKGCLLSGFRGNE
TTYLRPETDLETPPVLFIPNVHFSSLQRSGGAA
PSAGPSSSNRLPLKRTCSPFTEEFEPLPSKQAKE
GDLQRVLLYVRRETEEVFDALMLKTPDLKGLRN
AISEKYGFPEENIYKVYKKCKRGILVNMDNNII
QHYSNHVAFLLDMGELDGKIQIILKEL*GLSSIQ
TLTTCKGPAGTWPHATHNLSTCLSAVT*MPSLR
EEALESQTHRRQGQGET*GVPWPGSPWYA*ICS
LNFLPVPPRTPKQCHHGYHLPRRLFPPPKTLVCS
GAPAGCPLRWWRHTLPPAAPRQLVRPPLSVFLQ
LQTKDFSTFGQLT*KLLIFSANDF*KTLYWSLF
LRLPQRRM*IALTIDWNKVTAV*NYCLATHCCIH
FLFTIFICYIYI*IYCIYMQHFIFFMDMFLSFQ
KMCISHFLDFF*LLFSDAFCILTWYLVIKIYLC
ITSH
Frame 1
GDVPNC*EWLFLSRRMWMNSILPIFLVPRRSGYCPPALGAGMTKERGTTMAWNMRRTSLPLKAPHT
S*NS*QRTCLEPQSTQICSRRIT**AWRGPCPPL
ARQLPSLQAPASWRPALWTATCYPPLICMIMAP
STPCLRAFMGCRPHSAGSQTAPSKMTHRSRCSS
QIS*KPPRNPHVQRTTPASKVTLNTPWAPPKPST
SSQASHPWPTSTKASSTPSPCGPQQVAKALPCP
PTKSRVW*WLSSTMRRSQ*SSCASGSTGIPGNP
LPSSGSLTWLTAKKTSTLWSTLRRWPIMHCPLCG
T*MKRPRCSSA*TV*AQTFPHKRG*RVSP*TCR
LTPMTVAWALSAWYTVLSARSRSSVTRELRGRC
AMTSGSSSGGRSSALTPATVASRAACCRASGAMR
RPTFGQRLTWRRHPCCSSPMCTSPACSALEGQP
PRQDPAAPTGCL*SVPARPSLRSLSLCPPSRPR
KATFREFCCMCGGRLRRCLTRSC*RPQT*RG*GM
RSLRSMGSLKRTFTKSTRNASEES*STWTTTSF
SITATTSPSCWTWGSWTAKFRSSLRSCKASRAS
KPSRPARGQQGRGPTPHTTSPHASALLLECLP*G
KRPLSHRPTDVRARERPRGSPGLDPHGMLESAP
*TSCQCLPVPQNNVTMVTTYPEDCSLLPRPLSA
VVLLQAAR*DGGGTRSLPQHHASWCGPHSLSSFN
FRQRISQPLVS*LENS*FSVQMTFKRHYIGVSF
SDFLSAGCK*H*RSTGTK*PLCKTTALPLTVVY
ISYLRFSFVIYIYKYTVYICNILYFSWICFYHFK
KCVFHISWTFFSCYSVMHFVYSRGI***KSIYV
LRHI
Frame 2
EMCQTVKSGYF*AEECG*IPFFLFFWSQGEADIVLQHWGQE*PRKEVLPWHGI*DGPHSP*KPHTP
HEIPDRERVWNPRVPRFAQEE*PDELGGGLAHP
WQGSSPPCRPQQAGGRLCGQLPVTHH*YV**WLP
QLLV*EHSWGAAHTALAARQHLQR*PTGVDALP
RYPENLPGTPMSRGLPQPQK*L*IHPGLPQSHP
HQVRRVTHGLPQQRPVLPRHPADPSRWQRPCLVL
QQSQECGDGCLRQ*EGPSRAAALLEALAFPATH
CQAAGH*RG*LQRKLQHCGAH*GGGL*CTVLCV
ERE*RGQGVHRRKLSEHRLFLTKGGEGCPPEPAD
*HL*LWLGH*APGTPCCLPDQDLL*QGS*EEDA
R*RAEAVPEEGQVP*LQQQWRQGLPAVGLQGQ*
DDLPSARD*PGDATRAVHPQCALLQPAALWRGSP
LGRTQQLQQAASEAYLLALH*GV*ASALQAGQG
RRPSESSAVCAEGD*GGV*RAHVEDPRPEGAEE
CDL*EVWVP*REHLQSLQEMQARNLSQHGQQHHS
ALQQPRRLPAGHGGAGRQNSDHP*GAVRPLEHP
NPHDLQGASRDVAPRHTQPLHMPQRCYLNAFPE
GRGP*VTDPQTSGPGRDLGGPLAWIPMVCLNLLP
ELPASASPYPKTMSPWLPPTQKTVPSSQDPCLQ
WCSCRLPVKMVAAHAPSRSTTPAGAAPTLCLPS
TSDKGFLNLWSVNLKTLDFQCK*LLKDTILESLS
QTSSAQDVNSTNDRLEQSDRCVKLLPCHSLLYT
FLIYDFHLLYIYINILYIYATFYIFHGYVFIIS
KNVYFTFLGLFLAVIQ*CILYTHVVFSNKNLSMY
YVTL
Sequence NM_021180
AGGAGATGTGCCAAACTGTTAAGAGTGGTTATTTCTGAGCAGAAGAATGTGGATGAATTCCATTCTTCCTATTTTTCTTTTCAGGTCTGTGCGGCTGCTAAAGAACGACCCAGTCAACTTGCAGAAATTCTCTTACACTAGTGAGGATGAGGCCTGGAAGACGTACCTAGAAAACCCGTTGACAGCTGCCACAAAGGCCA
TGATGAGAGTCAATGGAGATGATGACAGTGTTGCGGCCTTGAGCTTCCTCTATGATTACTACATGGGTCCCAAGGAGAAGCGGATATTGTCCTCCAGCAC
TGGGGGCAGGAATGACCAAGGAAAGAGGTACTACCATGGCATGGAATATGAGACGGACCTCACTCCCCTTGAAAGCCCCACACACCTCATGAAATTCCTG
ACAGAGAACGTGTCTGGAACCCCAGAGTACCCAGATTTGCTCAAGAAGAATAACCTGATGAGCTTGGAGGGGGCCTTGCCCACCCCTGGCAAGGCAGCTC
CCCTCCCTGCAGGCCCCAGCAAGCTGGAGGCCGGCTCTGTGGACAGCTACCTGTTACCCACCACTGATATGTATGATAATGGCTCCCTCAACTCCTTGTT
TGAGAGCATTCATGGGGTGCCGCCCACACAGCGCTGGCAGCCAGACAGCACCTTCAAAGATGACCCACAGGAGTCGATGCTCTTCCCAGATATCCTGAAA
ACCTCCCCGGAACCCCCATGTCCAGAGGACTACCCCAGCCTCAAAAGTGACTTTGAATACACCCTGGGCTCCCCCAAAGCCATCCACATCAAGTCAGGCG
AGTCACCCATGGCCTACCTCAACAAAGGCCAGTTCTACCCCGTCACCCTGCGGACCCCAGCAGGTGGCAAAGGCCTTGCCTTGTCCTCCAACAAAGTCAA
GAGTGTGGTGATGGTTGTCTTCGACAATGAGAAGGTCCCAGTAGAGCAGCTGCGCTTCTGGAAGCACTGGCATTCCCGGCAACCCACTGCCAAGCAGCGG
GTCATTGACGTGGCTGACTGCAAAGAAAACTTCAACACTGTGGAGCACATTGAGGAGGTGGCCTATAATGCACTGTCCTTTGTGTGGAACGTGAATGAAG
AGGCCAAGGTGTTCATCGGCGTAAACTGTCTGAGCACAGACTTTTCCTCACAAAAGGGGGTGAAGGGTGTCCCCCTGAACCTGCAGATTGACACCTATGA
CTGTGGCTTGGGCACTGAGCGCCTGGTACACCGTGCTGTCTGCCAGATCAAGATCTTCTGTGACAAGGGAGCTGAGAGGAAGATGCGCGATGACGAGCGG
AAGCAGTTCCGGAGGAAGGTCAAGTGCCCTGACTCCAGCAACAGTGGCGTCAAGGGCTGCCTGCTGTCGGGCTTCAGGGGCAATGAGACGACCTACCTTC
GGCCAGAGACTGACCTGGAGACGCCACCCGTGCTGTTCATCCCCAATGTGCACTTCTCCAGCCTGCAGCGCTCTGGAGGGGCAGCCCCCTCGGCAGGACC
CAGCAGCTCCAACAGGCTGCCTCTGAAGCGTACCTGCTCGCCCTTCACTGAGGAGTTTGAGCCTCTGCCCTCCAAGCAGGCCAAGGAAGGCGACCTTCAG
AGAGTTCTGCTGTATGTGCGGAGGGAGACTGAGGAGGTGTTTGACGCGCTCATGTTGAAGACCCCAGACCTGAAGGGGCTGAGGAATGCGATCTCTGAGA
AGTATGGGTTCCCTGAAGAGAACATTTACAAAGTCTACAAGAAATGCAAGCGAGGAATCTTAGTCAACATGGACAACAACATCATTCAGCATTACAGCAA
CCACGTCGCCTTCCTGCTGGACATGGGGGAGCTGGACGGCAAAATTCAGATCATCCTTAAGGAGCTGTAAGGCCTCTCGAGCATCCAAACCCTCACGACC
TGCAAGGGGCCAGCAGGGACGTGGCCCCACGCCACACACAACCTCTCCACATGCCTCAGCGCTGTTACTTGAATGCCTTCCCTGAGGGAAGAGGCCCTTG
AGTCACAGACCCACAGACGTCAGGGCCAGGGAGAGACCTAGGGGGTCCCCTGGCCTGGATCCCCATGGTATGCTTGAATCTGCTCCCTGAACTTCCTGCC
AGTGCCTCCCCGTACCCCAAAACAATGTCACCATGGTTACCACCTACCCAGAAGACTGTTCCCTCCTCCCAAGACCCTTGTCTGCAGTGGTGCTCCTGCA
GGCTGCCCGTTAAGATGGTGGCGGCACACGCTCCCTCCCGCAGCACCACGCCAGCTGGTGCGGCCCCCACTCTCTGTCTTCCTTCAACTTCAGACAAAGG
ATTTCTCAACCTTTGGTCAGTTAACTTGAAAACTCTTGATTTTCAGTGCAAATGACTTTTAAAAGACACTATATTGGAGTCTCTTTCTCAGACTTCCTCA
GCGCAGGATGTAAATAGCACTAACGATCGACTGGAACAAAGTGACCGCTGTGTAAAACTACTGCCTTGCCACTCACTGTTGTATACATTTCTTATTTACG
ATTTTCATTTGTTATATATATATATAAATATACTGTATATATATGCAACATTTTATATTTTTCATGGATATGTTTTTATCATTTCAAAAAATGTGTATTT
CACATTTCTTGGACTTTTTTTAGCTGTTATTCAGTGATGCATTTTGTATACTCACGTGGTATTTAGTAATAAAAATCTATCTATGTATTACGTCACATTA
- refseq_GRHL3.F1 (forward primer)
- refseq_GRHL3.R1 (reverse primer)
- Single exon skipping
Frame 0
RRCAKLLRVVISEQKNVDEFHSSYFSFQVCAAAKERPSQLAEILLH**G*GLEDVPRKPVDSCHKGH
DESQWR**QCCGLELPL*LLHGSQGEADIVLQH
WGQE*PRKEVLPWHGI*DGPHSP*KPHTPHEIP
DRERVWNPRVPRFAQEE*PDELGGGLAHPWQGSS
PPCRPQQAGGRLCGQLPVTHH*YV**WLPQLLV
*EHSWGAAHTALAARQHLQR*PTGVDALPRYPE
NLPGTPMSRGLPQPQK*L*IHPGLPQSHPHQVRR
VTHGLPQQRPVLPRHPADPSRWQRPCLVLQQSQ
ECGDGCLRQ*EGPSRAAALLEALAFPATHCQAA
GH*RG*LQRKLQHCGAH*GGGL*CTVLCVERE*R
GQGVHRRKLSEHRLFLTKGGEGCPPEPAD*HL*
LWLGH*APGTPCCLPDQDLL*QGS*EEDAR*RA
EAVPEEGQVP*LQQQWRQGLPAVGLQGQ*DDLPS
ARD*PGDATRAVHPQCALLQPAALWRGSPLGRT
QQLQQAASEAYLLALH*GV*ASALQAGQGRRPS
ESSAVCAEGD*GGV*RAHVEDPRPEGAEECDL*E
VWVP*REHLQSLQEMQARNLSQHGQQHHSALQQ
PRRLPAGHGGAGRQNSDHP*GAVRPLEHPNPHD
LQGASRDVAPRHTQPLHMPQRCYLNAFPEGRGP*
VTDPQTSGPGRDLGGPLAWIPMVCLNLLPELPA
SASPYPKTMSPWLPPTQKTVPSSQDPCLQWCSC
RLPVKMVAAHAPSRSTTPAGAAPTLCLPSTSDKG
FLNLWSVNLKTLDFQCK*LLKDTILESLSQTSS
AQDVNSTNDRLEQSDRCVKLLPCHSLLYTFLIY
DFHLLYIYINILYIYATFYIFHGYVFIISKNVYF
TFLGLFLAVIQ*CILYTHVVFSNKNLSMYYVTL
Frame 1
GDVPNC*EWLFLSRRMWMNSILPIFLFRSVRLLKNDPVNLQKFSYTSEDEAWKTYLENPLTAATKA
MMRVNGDDDSVAALSFLYDYYMGPKEKRILSSST
GGRNDQGKRYYHGMEYETDLTPLESPTHLMKFL
TENVSGTPEYPDLLKKNNLMSLEGALPTPGKAA
PLPAGPSKLEAGSVDSYLLPTTDMYDNGSLNSLF
ESIHGVPPTQRWQPDSTFKDDPQESMLFPDILK
TSPEPPCPEDYPSLKSDFEYTLGSPKAIHIKSG
ESPMAYLNKGQFYPVTLRTPAGGKGLALSSNKVK
SVVMVVFDNEKVPVEQLRFWKHWHSRQPTAKQR
VIDVADCKENFNTVEHIEEVAYNALSFVWNVNE
EAKVFIGVNCLSTDFSSQKGVKGVPLNLQIDTYD
CGLGTERLVHRAVCQIKIFCDKGAERKMRDDER
KQFRRKVKCPDSSNSGVKGCLLSGFRGNETTYL
RPETDLETPPVLFIPNVHFSSLQRSGGAAPSAGP
SSSNRLPLKRTCSPFTEEFEPLPSKQAKEGDLQ
RVLLYVRRETEEVFDALMLKTPDLKGLRNAISE
KYGFPEENIYKVYKKCKRGILVNMDNNIIQHYSN
HVAFLLDMGELDGKIQIILKEL*GLSSIQTLTT
CKGPAGTWPHATHNLSTCLSAVT*MPSLREEAL
ESQTHRRQGQGET*GVPWPGSPWYA*ICSLNFLP
VPPRTPKQCHHGYHLPRRLFPPPKTLVCSGAPA
GCPLRWWRHTLPPAAPRQLVRPPLSVFLQLQTK
DFSTFGQLT*KLLIFSANDF*KTLYWSLFLRLPQ
RRM*IALTIDWNKVTAV*NYCLATHCCIHFLFT
IFICYIYI*IYCIYMQHFIFFMDMFLSFQKMCI
SHFLDFF*LLFSDAFCILTWYLVIKIYLCITSH
Frame 2
EMCQTVKSGYF*AEECG*IPFFLFFFSGLCGC*RTTQSTCRNSLTLVRMRPGRRT*KTR*QLPQRP
**ESMEMMTVLRP*ASSMITTWVPRRSGYCPPA
LGAGMTKERGTTMAWNMRRTSLPLKAPHTS*NS*
QRTCLEPQSTQICSRRIT**AWRGPCPPLARQL
PSLQAPASWRPALWTATCYPPLICMIMAPSTPC
LRAFMGCRPHSAGSQTAPSKMTHRSRCSSQIS*K
PPRNPHVQRTTPASKVTLNTPWAPPKPSTSSQA
SHPWPTSTKASSTPSPCGPQQVAKALPCPPTKS
RVW*WLSSTMRRSQ*SSCASGSTGIPGNPLPSSG
SLTWLTAKKTSTLWSTLRRWPIMHCPLCGT*MK
RPRCSSA*TV*AQTFPHKRG*RVSP*TCRLTPM
TVAWALSAWYTVLSARSRSSVTRELRGRCAMTSG
SSSGGRSSALTPATVASRAACCRASGAMRRPTF
GQRLTWRRHPCCSSPMCTSPACSALEGQPPRQD
PAAPTGCL*SVPARPSLRSLSLCPPSRPRKATFR
EFCCMCGGRLRRCLTRSC*RPQT*RG*GMRSLR
SMGSLKRTFTKSTRNASEES*STWTTTSFSITA
TTSPSCWTWGSWTAKFRSSLRSCKASRASKPSRP
ARGQQGRGPTPHTTSPHASALLLECLP*GKRPL
SHRPTDVRARERPRGSPGLDPHGMLESAP*TSC
QCLPVPQNNVTMVTTYPEDCSLLPRPLSAVVLLQ
AAR*DGGGTRSLPQHHASWCGPHSLSSFNFRQR
ISQPLVS*LENS*FSVQMTFKRHYIGVSFSDFL
SAGCK*H*RSTGTK*PLCKTTALPLTVVYISYLR
FSFVIYIYKYTVYICNILYFSWICFYHFKKCVF
HISWTFFSCYSVMHFVYSRGI***KSIYVLRHI
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