Sequence NM_152919
GGGGGGTCTGCTCTGTGCGGTGAAGCTTCTCTTCTTGGCACCTGCCTGGCATCGGAAGAGGGCCCCTTCTCCCTCCCTGGGCTTTTATGTGGACACTGTA
ATGCCTCAGTTTTCTTTCTTTCTTTTTGTTTTTGACACAGGGTCTCACTCTATCACCCAAGCTGGAGTGCAGTGGCACCATCTTAGCTCACCGCAGCCTC
AAACTCCCCAGCTCGGGTGATTCTTCCTGCCTCAGCCTCCTGAGTAGTAGCAGCTGGGACCACAGACGTCTGCCACCAAGCCCAGCTGCACCGCCAGTTC
CGGGGAGGGCCCTGGGCCAGCGGCTGTCCGCCCCCCCTCCTTTATAAAGTCCTGGCCTCGGGACAGCCCGCACAGCTGCCCAGCCTGCGGAGACGGGACA
GCCCTGTCCCACTCACTCTTTCCCCTGCTGCTCCTGCCGGCAGCTCAGCTGGAACCATGGGAGGCCGCGTCTTTCTCGTCTTTCTCGCATTCTGTGTCTG
GCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGGACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAAT
CCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCCATGGAGACTTGTGACGACATCAACGAGTGTGCAACACTGTCGAAAGTGTCATGCGGAAAAT
TCTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCAGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAA
CACGTGTCAAGATGTGGACGAATGTCAGCAGAACCCAAGGCTCTGTAAAAGCTACGGCACCTGCGTCAACACCCTCGGCAGCTACACGTGCCAGTGCCTG
CCTGGCTTCAAGCTCAAACCTGAGGACCCGAAGCTCTGCACAGATGTGAATGAATGCACCTCCGGACAAAACCCATGCCACAGCTCCACCCACTGCCTCA
ACAACGTGGGCAGCTATCAGTGCCGCTGCCGCCCGGGCTGGCAACCGATTCCGGGGTCCCCCAATGGCCCAAACAATACCGTCTGTGAAGATGTGGACGA
GTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATACAGCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACAC
GGAATCCCGAATAACCAAAAGGACACTGTCTGTGAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGATTCT
TCGACAAAGTCCAGGACCTGGGCAGAGACTACAAGCCAGGCTTGGCCAATAACACCATCCAGAGCATCTTACAGGCGCTGGATGAGCTGCTGGAGGCCCC
TGGGGACCTGGAGACCCTGCCCCGCTTACAGCAGCACTGTGTGGCCAGTCACCTGCTGGATGGCCTAGAGGATGTCCTCAGAGGCCTGAGCAAGAACCTT
TCCAATGGGCTGTTGAACTTCAGTTATCCTGCAGGCACAGAATTGTCCCTGGAGGTGCAGAAGCAAGTAGACAGGAGTGTCACCTTGAGACAGAATCAGG
CAGTGATGCAGCTCGACTGGAATCAGGCACAGAAATCTGGTGACCCAGGGATGGGCAAGTTGCTGGCTGAGGCCCCTCTGGTCCTGGAACCTGAGAAGCA
GATGCTTCTGCATGAGACACACCAGGGCTTGCTGCAGGACGGCTCCCCCATCCTGCTCTCAGATGTGATCTCTGCCTTTCTGAGCAACAACGACACCCAA
AACCTCAGCTCCCCAGTTACCTTCACCTTCTCCCACCGTTCAGTGATCCCGAGACAGAAGGTGCTCTGTGTCTTCTGGGAGCATGGCCAGAATGGATGTG
GTCACTGGGCCACCACAGGCTGCAGCACAATAGGCACCAGAGACACCAGCACCATCTGCCGTTGCACCCACCTGAGCAGCTTTGCCGTCCTCATGGCCCA
CTACGATGTGCAGGAGGAGGATCCCGTGCTGACTGTCATCACCTACATGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTCCTGGCGGCCCTCACTTTTCTC
CTGTGTAAAGCCATCCAGAACACCAGCACCTCACTGCATCTGCAGCTCTCGCTCTGCCTCTTCCTGGCCCACCTCCTCTTCCTCGTGGCAATTGATCAAA
CCGGACACAAGGTGCTGTGCTCCATCATCGCCGGTACCTTGCACTATCTCTACCTGGCCACCTTGACCTGGATGCTGCTGGAGGCCCTGTACCTCTTCCT
CACTGCACGGAACCTGACGGTGGTCAACTACTCAAGCATCAACAGATTCATGAAGAAGCTCATGTTCCCTGTGGGCTACGGAGTCCCAGCTGTGACAGTG
GCCATTTCTGCAGCCTCCAGGCCTCACCTTTATGGAACACCTTCCCGCTGCTGGCTCCAACCAGAAAAGGGATTTATATGGGGCTTCCTTGGACCTGTCT
GCGCCATCTTCTCTGTGAATTTAGTTCTCTTTCTGGTGACTCTCTGGATTTTGAAAAACAGACTCTCCTCCCTCAATAGTGAAGTGTCCACCCTCCGGAA
CACAAGGATGCTGGCATTTAAAGCGACAGCTCAGCTGTTCATCCTGGGCTGCACGTGGTGTCTGGGCATCTTGCAGGTGGGTCCGGCTGCCCGGGTCATG
GCCTACCTCTTCACCATCATCAACAGCCTGCAGGGTGTCTTCATCTTCCTGGTGTACTGCCTCCTCAGCCAGCAGGTCCGGGAGCAATATGGGAAATGGT
CCAAAGGGATCAGGAAATTGAAAACTGAGTCTGAGATGCACACACTCTCCAGCAGTGCTAAGGCTGACACCTCCAAACCCAGCACGGTTAACTAGAAAAA
TCTTCTGAATAAGATCTTCCCTCTTTGCCCGTGGAAAATCTGAACAATCTTTGAGCCATCTAGAGGGGAAAGAAAAGACTTTGTTCTGTGTGTTTCAAGA
AATTCACCATGTCAGCAATATGAAGGATGTTATGGAAGGCGTGCTAGGCATTCAATTCCTGCAGAAACCGGAAATCTTCCATGCCCTGCAATGTGCTCAT
CAAACTCTCAGCATATGGACGGCCAGCTGTGGCCCATATCTTGGTCACTCTGAAGCACAATATTTATGAAGCTATAGAACGTTAAGACCTCTTTCACAGC
CTCTCCTTCCTACAAAGACTCCTCCAAATCTTAAAATGAAGCAGGAAAACGAGCCTAAGAGGACTTTCATACCGACAACATCTGAAAGGACTAGAATGTT
CACACCACGATCTGGATTTCTTAATTTTTTGTTTTTGTTTTTGTTGTTCTCTAGTTCTACGGGTTTGATTATTTAGTCATGTGAAAAATATTGATTACTC
ACACATAGATCAAGAGAGACACGGCTCCTGCCTTCATGGAGCTTTTAGGGGAAAATGAAGTGGCTCTTGCAGCTAGAGTTGACTCAGAAGCCGAAATTCC
TAGAAATCAGGTTTCTACTGCTAGGCAATTGAAGTATAAACTATTTTATAAACACTGTCTTCTTTCGTCTTCACACCAACATGCAGAAAAGTTTCTAATC
TCAGATCGGGGATGTGCAACAAATTCCATTTCAAAGGAATGACCTGCAAAACTCCTAAATATTCCAAGCAAATGCCCTTAACCCTGTCTGTTATCTGCTT
TCCTTGAACAGAAATTCTACATGACCATAAAACCTCGAAGATGGGTATGGCACAGTTCATGCCCTGTAATCCTAGCACTTTGGGAGGGTGAGGCAGGAGG
ATGGCTCAAGCCCAGGAGTTTGAGACCAGTGTGGGCAACAGAGTGAGAACCATCTCTACCCAAAAAAAAAATTAAAAATTAGCCAAGCATGGTGATGATA
TAGGAGTTAAGGAGAAATCATTTAGGCAAATAGCAAGGGTAGGAAGTCCTCAGTAAGGTTTTCCATTTAATGAAAAGCAGCCCCCAAAATCATTTTCTTT
TCTAACAAAGAACAGCCTGTAAAATCGAGCTGCAGACATAGACAAGCAAGCTGGAAGCTTCCACGGGTGAATGCCGGCAGCTGTGCCAATAGGAAAAAGC
TACCTAGACTAGGCATGTCCAAAATGGCGGCTCCAAGTTCCCTTCTCTTTGCCAGCCATGTGTACAGTAAAAAGCAGGCAACATAGTGTCAGCCAAAGCT
CATTTGCATAATAAGATTAGGGTGGGGTGGCCAGCTCACATAGGGGTAGGCCCTAGGTAAATCAGACACCGCCTTCTCAAGCCTGTCTATAAAATCTGGT
ACACTATGACGAGGGTCAGATTTCCCATTCAGACGCCCCTCTCCCATGCAAGAGAAAGAGCTGTTCTCCTTTCTCTTTCTTTTGCCTATTAAACCTCTGC
TCCTGGCCAGGCACAGTGGCTCACGCCTATAATCCCAGCACTTTGGGAGGCTGAGGTGGTCAGATCACCTAAGGTCAGGAGTTCAAGACCAGCCTGGTCA
ACATGGTGAAATCTTGTCTCTAGTAAAAATACAAAAATATATGAAATCTCACATAGATGATAATATTAAGTTCCAAAAGCAACTCAACCTGGTAGATTCT
AATTTTTTTTGAGGCAGGGTCTTGCTTTGTCACCCATGCTGGAGTACAATGGCACAAACACTGCTCACTGCAGCCTCGACCTCCCAAGGCCTAAGCAATC
CTCCTGCCTCAGTCCCCCTCCAGGTATTTGAAACTACAGGTGTGTACCACCACACCGGGCTAATTTTTGTATTTTTTGTAGAGACGTGGGTCTCACTATG
CTGCCCAGGCTCAGGTCTTAATCTCCTGAGCTCAGGCAATCCGCAGGCCTCAGCCTCCCTAAGTGCGGGGATTACAGGCTTGAGCCACTGCACCTAGCCT
CTATTTGTTTTACAAAAGAGAAATTGAGATCCTGAATGTTAAGTGACTTGCCTGAGGCCATCCCACTAACAGGAGCCAGGGTTAGGATTCAAACCCCATC
CAACTGGTCCCAGAGCTGGAGCTTCTTGCACTGCCCTACACTACCTACCATCTCCATCCTCTGGGCACCTTTTTATAAGAACCAAAACATTACAGAGCAT
TGCTTTGTCAACTCAGCTGGGAACATTTCCCAGTGCAACTCACATTTTTCACTGCTCTGTGCCTGTCCGTATAAGCTCAATGAGTATTGATTTAGGGGCT
TTGGAGAACTTTGAATGCTACCCCCCAAGTAACCATTGTTGGCAACCTGGTACCTCTACTTTTAGCCATTTCTCCTTCTCTATAAATAGTGCAGAAGTAA
CCCACTTGGTAACAGGCATCCTTGCCAAGCCTCCACCACTAGGTCAGTGTAAGAATTAAAGAAAGAGGAAAGAAACACAAAAAGTGGCTTGATGGTTAAG
ACAGGTTTATTTTAGAGAAAACACACCTGAGAGGGGCTGCTGGCTGAATTAGGTTAGAGTCTTTTCTACAGACTAAGAGTGTTTAAGGATTTAGGGTGGG
AGAGTTTCTTAGAGGCTTGGACTGCTTCTGTGTTTTTTTTGTTGTGCTTATATGGGAGGGAGAGTGGTGTGTTTGCTTTTATACATTTTTCTGCAGCTGT
AGGCATACCCCCCAAGTCTGCTTTTAGCTTCCCTATTTTAGTGCACCTGGAGGGAAAGGAATGTGCTTATTAAGGCCCACTGTTTTACTGGGGCCCATTG
TATGAGGGTGAAGTTTGGCAGTTACCCAAGAGACTTTTCCTCCACCTTCCTCTGTGCCCGAGCTGTTTTATCTGCATTTTACTGTCTGCTTTTTTTGGCT
GCTTATAGTTTTTAAAAAAGTAATTTCCTTAAATCCAGAAGGCTAAAAATGAAGCTGAAACTTAAAGTGGCGGTGTTTGTCCAAAATAACGGGGCTCCTG
CTCTGCCAGTCAGTACCCTCAAGTCACTCCTGATCCTCAACCTCCATGCCTAAGGCTGGTTCAAGAGACCACATAATATCTGCCTTTTATTACATACATG
ATGGGTGCATGGGATTCTGCGTGCCCTTTGCTTGATATAGACTGCTAAGGTGAGATGGGGAATATCAGAGTCAGCTGCTGCTTGAGGAAGCAGAACACAC
AGCTGGAGGCTTGGAACATGTGGGTCCCTATGAGTGTAGAGCCCATATCCCCATAGAGTCTACCTAGAGCAGGGGTCGCCAAATGTTTTCTTAAAGAGCC
TGATAGTGTATATGTTAGGCTTTGTGAGCCAGGTATTTACAGCAACTCAATTCTACCACTGTGGTATGAAAACAGCTATAGACAATCATAAATGAATGAT
CATGGCTATGTTTTAATAAAACTTTACAGACACTGAACTTGAACTTCCATTGTGATATGAAAACAGCTATAGACAATCATAAATGAATGATCATGGCTAT
GTTTTAATAAAACTTTATGGACACTGAGCTTGAACTTCATATATCAGTCATGTGACACAAAATATCATTCTTCTTTTTATTTGTTTTCACCCATTTGAAA
AATGTAAAAACTATTCTTAGCTGTACAGAAACAGATGGTGGGTCAGATTTGACTCACTGCCCATAGTTTCCAGACCATGATGTTCAGGTTCATGGAAGCA
CTTCACTCTACACTAATTTATTCATCCATTCATCACATATGTGATGAGCACATACAGACACACCTCAGAGATATTGCAGGCTCCACTCCAGATCACCACA
ATAATGTGAATATTGCAATAAAGCAAGTCATACACATT
- refseq_EMR2.F3 (forward primer)
- refseq_EMR2.R3 (reverse primer)
- Alternative acceptor
Frame 0
GGSALCGEASLLGTCLASEEGPFSLPGLLCGHC
NASVFFLSFCF*HRVSLYHPSWSAVAPS*LTAAS
NSPARVILPASAS*VVAAGTTDVCHQAQLHRQF
RGGPWASGCPPPLLYKVLASGQPAQLPSLRRRD
SPVPLTLSPAAPAGSSAGTMGGRVFLVFLAFCVW
LTLPGAETQDSRGCARWCPQDSSCVNATACRCN
PGFSSFSEIITTPMETCDDINECATLSKVSCGK
FSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESEN
TCQDVDECQQNPRLCKSYGTCVNTLGSYTCQCL
PGFKLKPEDPKLCTDVNECTSGQNPCHSSTHCL
NNVGSYQCRCRPGWQPIPGSPNGPNNTVCEDVDE
CSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRH
GIPNNQKDTVCEDMTFSTWTPPPGVHSQTLSRF
FDKVQDLGRDYKPGLANNTIQSILQALDELLEAP
GDLETLPRLQQHCVASHLLDGLEDVLRGLSKNL
SNGLLNFSYPAGTELSLEVQKQVDRSVTLRQNQ
AVMQLDWNQAQKSGDPGMGKLLAEAPLVLEPEKQ
MLLHETHQGLLQDGSPILLSDVISAFLSNNDTQ
NLSSPVTFTFSHRSVIPRQKVLCVFWEHGQNGC
GHWATTGCSTIGTRDTSTICRCTHLSSFAVLMAH
YDVQEEDPVLTVITYMGLSVSLLCLLLAALTFL
LCKAIQNTSTSLHLQLSLCLFLAHLLFLVAIDQ
TGHKVLCSIIAGTLHYLYLATLTWMLLEALYLFL
TARNLTVVNYSSINRFMKKLMFPVGYGVPAVTV
AISAASRPHLYGTPSRCWLQPEKGFIWGFLGPV
CAIFSVNLVLFLVTLWILKNRLSSLNSEVSTLRN
TRMLAFKATAQLFILGCTWCLGILQVGPAARVM
AYLFTIINSLQGVFIFLVYCLLSQQVREQYGKW
SKGIRKLKTESEMHTLSSSAKADTSKPSTVN*KN
LLNKIFPLCPWKI*TIFEPSRGERKDFVLCVSR
NSPCQQYEGCYGRRARHSIPAETGNLPCPAMCS
SNSQHMDGQLWPISWSL*STIFMKL*NVKTSFTA
SPSYKDSSKS*NEAGKRA*EDFHTDNI*KD*NV
HTTIWIS*FFVFVFVVL*FYGFDYLVM*KILIT
HT*IKRDTAPAFMELLGENEVALAARVDSEAEIP
RNQVSTARQLKYKLFYKHCLLSSSHQHAEKFLI
SDRGCATNSISKE*PAKLLNIPSKCP*PCLLSA
FLEQKFYMTIKPRRWVWHSSCPVILALWEGEAGG
WLKPRSLRPVWATE*EPSLPKKKIKN*PSMVMI
*ELRRNHLGK*QG*EVLSKVFHLMKSSPQNHFL
F*QRTACKIELQT*TSKLEASTGECRQLCQ*EKA
T*TRHVQNGGSKFPSLCQPCVQ*KAGNIVSAKA
HLHNKIRVGWPAHIGVGPR*IRHRLLKPVYKIW
YTMTRVRFPIQTPLSHARERAVLLSLSFAY*TSA
PGQAQWLTPIIPALWEAEVVRSPKVRSSRPAWS
TW*NLVSSKNTKIYEISHR**Y*VPKATQPGRF
*FFLRQGLALSPMLEYNGTNTAHCSLDLPRPKQS
SCLSPPPGI*NYRCVPPHRANFCIFCRDVGLTM
LPRLRS*SPELRQSAGLSLPKCGDYRLEPLHLA
SICFTKEKLRS*MLSDLPEAIPLTGARVRIQTPS
NWSQSWSFLHCPTLPTISILWAPFYKNQNITEH
CFVNSAGNISQCNSHFSLLCACPYKLNEY*FRG
FGEL*MLPPK*PLLATWYLYF*PFLLLYK*CRSN
PLGNRHPCQASTTRSV*ELKKEERNTKSGLMVK
TGLF*RKHT*EGLLAELG*SLFYRLRVFKDLGW
ESFLEAWTASVFFLLCLYGRESGVFAFIHFSAAV
GIPPKSAFSFPILVHLEGKECAY*GPLFYWGPL
YEGEVWQLPKRLFLHLPLCPSCFICILLSAFFG
CL*FLKK*FP*IQKAKNEAET*SGGVCPK*RGSC
SASQYPQVTPDPQPPCLRLVQETT*YLPFITYM
MGAWDSACPLLDIDC*GEMGNIRVSCCLRKQNT
QLEAWNMWVPMSVEPISP*SLPRAGVAKCFLKEP
DSVYVRLCEPGIYSNSILPLWYENSYRQS*MND
HGYVLIKLYRH*T*TSIVI*KQL*TIINE*SWL
CFNKTLWTLSLNFIYQSCDTKYHSSFYLFSPI*K
M*KLFLAVQKQMVGQI*LTAHSFQTMMFRFMEA
LHSTLIYSSIHHICDEHIQTHLRDIAGSTPDHH
NNVNIAIKQVIHI
Frame 1
GGLLCAVKLLFLAPAWHRKRAPSPSLGFYVDTV
MPQFSFFLFVFDTGSHSITQAGVQWHHLSSPQP
QTPQLG*FFLPQPPE**QLGPQTSATKPSCTASS
GEGPGPAAVRPPSFIKSWPRDSPHSCPACGDGT
ALSHSLFPLLLLPAAQLEPWEAASFSSFSHSVS
G*LCRELKPRTPGAVPGGALRTPRVSMPPPVAAI
QGSALFLRSSPPPWRLVTTSTSVQHCRKCHAEN
SRTAGTQRGATTACAAQDMSLFLGQKHSRMRAR
TRVKMWTNVSRTQGSVKATAPASTPSAATRASAC
LASSSNLRTRSSAQM*MNAPPDKTHATAPPTAS
TTWAAISAAAARAGNRFRGPPMAQTIPSVKMWT
SAAPGSISVTAPPSASTPWVHTAAAAAQAGSPDT
ESRITKRTLSVKI*LSPPGPRPLESTARRFPDS
STKSRTWAETTSQAWPITPSRASYRRWMSCWRP
LGTWRPCPAYSSTVWPVTCWMA*RMSSEA*ARTF
PMGC*TSVILQAQNCPWRCRSK*TGVSP*DRIR
Q*CSSTGIRHRNLVTQGWASCWLRPLWSWNLRS
RCFCMRHTRACCRTAPPSCSQM*SLPF*ATTTPK
TSAPQLPSPSPTVQ*SRDRRCSVSSGSMARMDV
VTGPPQAAAQ*APETPAPSAVAPT*AALPSSWP
TTMCRRRIPC*LSSPTWG*ASLCCASSWRPSLFS
CVKPSRTPAPHCICSSRSASSWPTSSSSWQLIK
PDTRCCAPSSPVPCTISTWPP*PGCCWRPCTSS
SLHGT*RWSTTQASTDS*RSSCSLWATESQL*QW
PFLQPPGLTFMEHLPAAGSNQKRDLYGASLDLS
APSSL*I*FSFW*LSGF*KTDSPPSIVKCPPSG
TQGCWHLKRQLSCSSWAARGVWASCRWVRLPGSW
PTSSPSSTACRVSSSSWCTASSASRSGSNMGNG
PKGSGN*KLSLRCTHSPAVLRLTPPNPARLTRK
IF*IRSSLFARGKSEQSLSHLEGKEKTLFCVFQE
IHHVSNMKDVMEGVLGIQFLQKPEIFHALQCAH
QTLSIWTASCGPYLGHSEAQYL*SYRTLRPLSQ
PLLPTKTPPNLKMKQENEPKRTFIPTTSERTRMF
TPRSGFLNFLFLFLLFSSSTGLII*SCEKY*LL
THRSRETRLLPSWSF*GKMKWLLQLELTQKPKF
LEIRFLLLGN*SINYFINTVFFRLHTNMQKSF*S
QIGDVQQIPFQRNDLQNS*IFQANALNPVCYLL
SLNRNST*P*NLEDGYGTVHAL*S*HFGRVRQE
DGSSPGV*DQCGQQSENHLYPKKKLKISQAW**Y
RS*GEII*ANSKGRKSSVRFSI**KAAPKIIFF
SNKEQPVKSSCRHRQASWKLPRVNAGSCANRKK
LPRLGMSKMAAPSSLLFASHVYSKKQAT*CQPKL
ICIIRLGWGGQLT*G*ALGKSDTAFSSLSIKSG
TL*RGSDFPFRRPSPMQEKELFSFLFLLPIKPL
LLARHSGSRL*SQHFGRLRWSDHLRSGVQDQPGQ
HGEILSLVKIQKYMKSHIDDNIKFQKQLNLVDS
NFF*GRVLLCHPCWSTMAQTLLTAASTSQGLSN
PPASVPLQVFETTGVYHHTGLIFVFFVETWVSLC
CPGSGLNLLSSGNPQASASLSAGITGLSHCT*P
LFVLQKRN*DPEC*VTCLRPSH*QEPGLGFKPH
PTGPRAGASCTALHYLPSPSSGHLFIRTKTLQSI
ALSTQLGTFPSATHIFHCSVPVRISSMSIDLGA
LENFECYPPSNHCWQPGTSTFSHFSFSINSAEV
THLVTGILAKPPPLGQCKN*RKRKETQKVA*WLR
QVYFRENTPERGCWLN*VRVFSTD*ECLRI*GG
RVS*RLGLLLCFFCCAYMGGRVVCLLLYIFLQL
*AYPPSLLLASLF*CTWRERNVLIKAHCFTGAHC
MRVKFGSYPRDFSSTFLCARAVLSAFYCLLFLA
AYSF*KSNFLKSRRLKMKLKLKVAVFVQNNGAP
ALPVSTLKSLLILNLHA*GWFKRPHNICLLLHT*
WVHGILRALCLI*TAKVRWGISESAAA*GSRTH
SWRLGTCGSL*V*SPYPHRVYLEQGSPNVFLKS
LIVYMLGFVSQVFTATQFYHCGMKTAIDNHK*MI
MAMF**NFTDTELELPL*YENSYRQS*MNDHGY
VLIKLYGH*A*TSYISHVTQNIILLFICFHPFE
KCKNYS*LYRNRWWVRFDSLPIVSRP*CSGSWKH
FTLH*FIHPFITYVMSTYRHTSEILQAPLQITT
IM*ILQ*SKSYT
Frame 2
GVCSVR*SFSSWHLPGIGRGPLLPPWAFMWTL*
CLSFLSFFLFLTQGLTLSPKLECSGTILAHRSL
KLPSSGDSSCLSLLSSSSWDHRRLPPSPAAPPV
PGRALGQRLSAPPPL*SPGLGTARTAAQPAETGQ
PCPTHSFPCCSCRQLSWNHGRPRLSRLSRILCL
ADSAGS*NPGLQGLCPVVPSGLLVCQCHRLSLQ
SRVQLFF*DHHHPHGDL*RHQRVCNTVESVMRKI
LGLLEHRGELRLRVQPRI*ACFWGKNIQE*ERE
HVSRCGRMSAEPKAL*KLRHLRQHPRQLHVPVP
AWLQAQT*GPEALHRCE*MHLRTKPMPQLHPLPQ
QRGQLSVPLPPGLATDSGVPQWPKQYRL*RCGR
VQLRAASV*QLHRLLQHRGFIQLPLPPRLEAQT
RNPE*PKGHCL*RYDFLHLDPAPWSPQPDAFPIL
RQSPGPGQRLQARLGQ*HHPEHLTGAG*AAGGP
WGPGDPAPLTAALCGQSPAGWPRGCPQRPEQEP
FQWAVELQLSCRHRIVPGGAEASRQECHLETESG
SDAARLESGTEIW*PRDGQVAG*GPSGPGT*EA
DASA*DTPGLAAGRLPHPALRCDLCLSEQQRHP
KPQLPSYLHLLPPFSDPETEGALCLLGAWPEWMW
SLGHHRLQHNRHQRHQHHLPLHPPEQLCRPHGP
LRCAGGGSRADCHHLHGAERLSAVPPPGGPHFS
PV*SHPEHQHLTASAALALPLPGPPPLPRGN*SN
RTQGAVLHHRRYLALSLPGHLDLDAAGGPVPLP
HCTEPDGGQLLKHQQIHEEAHVPCGLRSPSCDS
GHFCSLQASPLWNTFPLLAPTRKGIYMGLPWTCL
RHLLCEFSSLSGDSLDFEKQTLLPQ**SVHPPE
HKDAGI*SDSSAVHPGLHVVSGHLAGGSGCPGH
GLPLHHHQQPAGCLHLPGVLPPQPAGPGAIWEMV
QRDQEIEN*V*DAHTLQQC*G*HLQTQHG*LEK
SSE*DLPSLPVENLNNL*AI*RGKKRLCSVCFK
KFTMSAI*RMLWKAC*AFNSCRNRKSSMPCNVLI
KLSAYGRPAVAHILVTLKHNIYEAIER*DLFHS
LSFLQRLLQILK*SRKTSLRGLSYRQHLKGLEC
SHHDLDFLIFCFCFCCSLVLRV*LFSHVKNIDYS
HIDQERHGSCLHGAFRGK*SGSCS*S*LRSRNS
*KSGFYC*AIEV*TIL*TLSSFVFTPTCRKVSN
LRSGMCNKFHFKGMTCKTPKYSKQMPLTLSVICF
P*TEILHDHKTSKMGMAQFMPCNPSTLGG*GRR
MAQAQEFETSVGNRVRTISTQKKN*KLAKHGDD
IGVKEKSFRQIARVGSPQ*GFPFNEKQPPKSFSF
LTKNSL*NRAADIDKQAGSFHG*MPAAVPIGKS
YLD*ACPKWRLQVPFSLPAMCTVKSRQHSVSQS
SFA**D*GGVASSHRGRP*VNQTPPSQACL*NLV
HYDEGQISHSDAPLPCKRKSCSPFSFFCLLNLC
SWPGTVAHAYNPSTLGG*GGQIT*GQEFKTSLV
NMVKSCL**KYKNI*NLT*MIILSSKSNSTW*IL
IFFEAGSCFVTHAGVQWHKHCSLQPRPPKA*AI
LLPQSPSRYLKLQVCTTTPG*FLYFL*RRGSHY
AAQAQVLIS*AQAIRRPQPP*VRGLQA*ATAPSL
YLFYKREIEILNVK*LA*GHPTNRSQG*DSNPI
QLVPELELLALPYTTYHLHPLGTFL*EPKHYRA
LLCQLSWEHFPVQLTFFTALCLSV*AQ*VLI*GL
WRTLNATPQVTIVGNLVPLLLAISPSL*IVQK*
PTW*QASLPSLHH*VSVRIKERGKKHKKWLDG*
DRFILEKTHLRGAAG*IRLESFLQTKSV*GFRVG
EFLRGLDCFCVFFVVLIWEGEWCVCFYTFFCSC
RHTPQVCF*LPYFSAPGGKGMCLLRPTVLLGPI
V*G*SLAVTQETFPPPSSVPELFYLHFTVCFFWL
LIVFKKVISLNPEG*K*S*NLKWRCLSKITGLL
LCQSVPSSHS*SSTSMPKAGSRDHIISAFYYIH
DGCMGFCVPFA*YRLLR*DGEYQSQLLLEEAEHT
AGGLEHVGPYECRAHIPIEST*SRGRQMFS*RA
**CIC*AL*ARYLQQLNSTTVV*KQL*TIINE*
SWLCFNKTLQTLNLNFHCDMKTAIDNHK*MIMAM
F**NFMDTELELHISVM*HKISFFFLFVFTHLK
NVKTILSCTETDGGSDLTHCP*FPDHDVQVHGS
TSLYTNLFIHSSHM**AHTDTPQRYCRLHSRSPQ
*CEYCNKASHTH
Sequence NM_152921
GGGGGGTCTGCTCTGTGCGGTGAAGCTTCTCTTCTTGGCACCTGCCTGGCATCGGAAGAGGGCCCCTTCTCCCTCCCTGGGCTTTTATGTGGACACTGTA
ATGCCTCAGTTTTCTTTCTTTCTTTTTGTTTTTGACACAGGGTCTCACTCTATCACCCAAGCTGGAGTGCAGTGGCACCATCTTAGCTCACCGCAGCCTC
AAACTCCCCAGCTCGGGTGATTCTTCCTGCCTCAGCCTCCTGAGTAGTAGCAGCTGGGACCACAGACGTCTGCCACCAAGCCCAGCTGCACCGCCAGTTC
CGGGGAGGGCCCTGGGCCAGCGGCTGTCCGCCCCCCCTCCTTTATAAAGTCCTGGCCTCGGGACAGCCCGCACAGCTGCCCAGCCTGCGGAGACGGGACA
GCCCTGTCCCACTCACTCTTTCCCCTGCTGCTCCTGCCGGCAGCTCAGCTGGAACCATGGGAGGCCGCGTCTTTCTCGTCTTTCTCGCATTCTGTGTCTG
GCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGGACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAAT
CCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCCATGGAGACTTGTGACGACATCAACGAGTGTGCAACACTGTCGAAAGTGTCATGCGGAAAAT
TCTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCAGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAA
CACGTGTCAAGATGTGGACGAGTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATACAGCTGCCGCTGCCGC
CCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACACTGTCTGTGAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACA
GCCAGACGCTTTCCCGATTCTTCGACAAAGTCCAGGACCTGGGCAGAGACTACAAGCCAGGCTTGGCCAATAACACCATCCAGAGCATCTTACAGGCGCT
GGATGAGCTGCTGGAGGCCCCTGGGGACCTGGAGACCCTGCCCCGCTTACAGCAGCACTGTGTGGCCAGTCACCTGCTGGATGGCCTAGAGGATGTCCTC
AGAGGCCTGAGCAAGAACCTTTCCAATGGGCTGTTGAACTTCAGTTATCCTGCAGGCACAGAATTGTCCCTGGAGGTGCAGAAGCAAGTAGACAGGAGTG
TCACCTTGAGACAGAATCAGGCAGTGATGCAGCTCGACTGGAATCAGGCACAGAAATCTGGTGACCCAGGGATGGGCAAGTTGCTGGCTGAGGCCCCTCT
GGTCCTGGAACCTGAGAAGCAGATGCTTCTGCATGAGACACACCAGGGCTTGCTGCAGGACGGCTCCCCCATCCTGCTCTCAGATGTGATCTCTGCCTTT
CTGAGCAACAACGACACCCAAAACCTCAGCTCCCCAGTTACCTTCACCTTCTCCCACCGTTCAGTGATCCCGAGACAGAAGGTGCTCTGTGTCTTCTGGG
AGCATGGCCAGAATGGATGTGGTCACTGGGCCACCACAGGCTGCAGCACAATAGGCACCAGAGACACCAGCACCATCTGCCGTTGCACCCACCTGAGCAG
CTTTGCCGTCCTCATGGCCCACTACGATGTGCAGGAGGAGGATCCCGTGCTGACTGTCATCACCTACATGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTC
CTGGCGGCCCTCACTTTTCTCCTGTGTAAAGCCATCCAGAACACCAGCACCTCACTGCATCTGCAGCTCTCGCTCTGCCTCTTCCTGGCCCACCTCCTCT
TCCTCGTGGCAATTGATCAAACCGGACACAAGGTGCTGTGCTCCATCATCGCCGGTACCTTGCACTATCTCTACCTGGCCACCTTGACCTGGATGCTGCT
GGAGGCCCTGTACCTCTTCCTCACTGCACGGAACCTGACGGTGGTCAACTACTCAAGCATCAACAGATTCATGAAGAAGCTCATGTTCCCTGTGGGCTAC
GGAGTCCCAGCTGTGACAGTGGCCATTTCTGCAGCCTCCAGGCCTCACCTTTATGGAACACCTTCCCGCTGCTGGCTCCAACCAGAAAAGGGATTTATAT
GGGGCTTCCTTGGACCTGTCTGCGCCATCTTCTCTGTGAATTTAGTTCTCTTTCTGGTGACTCTCTGGATTTTGAAAAACAGACTCTCCTCCCTCAATAG
TGAAGTGTCCACCCTCCGGAACACAAGGATGCTGGCATTTAAAGCGACAGCTCAGCTGTTCATCCTGGGCTGCACGTGGTGTCTGGGCATCTTGCAGGTG
GGTCCGGCTGCCCGGGTCATGGCCTACCTCTTCACCATCATCAACAGCCTGCAGGGTGTCTTCATCTTCCTGGTGTACTGCCTCCTCAGCCAGCAGGTCC
GGGAGCAATATGGGAAATGGTCCAAAGGGATCAGGAAATTGAAAACTGAGTCTGAGATGCACACACTCTCCAGCAGTGCTAAGGCTGACACCTCCAAACC
CAGCACGGTTAACTAGAAAAATCTTCTGAATAAGATCTTCCCTCTTTGCCCGTGGAAAATCTGAACAATCTTTGAGCCATCTAGAGGGGAAAGAAAAGAC
TTTGTTCTGTGTGTTTCAAGAAATTCACCATGTCAGCAATATGAAGGATGTTATGGAAGGCGTGCTAGGCATTCAATTCCTGCAGAAACCGGAAATCTTC
CATGCCCTGCAATGTGCTCATCAAACTCTCAGCATATGGACGGCCAGCTGTGGCCCATATCTTGGTCACTCTGAAGCACAATATTTATGAAGCTATAGAA
CGTTAAGACCTCTTTCACAGCCTCTCCTTCCTACAAAGACTCCTCCAAATCTTAAAATGAAGCAGGAAAACGAGCCTAAGAGGACTTTCATACCGACAAC
ATCTGAAAGGACTAGAATGTTCACACCACGATCTGGATTTCTTAATTTTTTGTTTTTGTTTTTGTTGTTCTCTAGTTCTACGGGTTTGATTATTTAGTCA
TGTGAAAAATATTGATTACTCACACATAGATCAAGAGAGACACGGCTCCTGCCTTCATGGAGCTTTTAGGGGAAAATGAAGTGGCTCTTGCAGCTAGAGT
TGACTCAGAAGCCGAAATTCCTAGAAATCAGGTTTCTACTGCTAGGCAATTGAAGTATAAACTATTTTATAAACACTGTCTTCTTTCGTCTTCACACCAA
CATGCAGAAAAGTTTCTAATCTCAGATCGGGGATGTGCAACAAATTCCATTTCAAAGGAATGACCTGCAAAACTCCTAAATATTCCAAGCAAATGCCCTT
AACCCTGTCTGTTATCTGCTTTCCTTGAACAGAAATTCTACATGACCATAAAACCTCGAAGATGGGTATGGCACAGTTCATGCCCTGTAATCCTAGCACT
TTGGGAGGGTGAGGCAGGAGGATGGCTCAAGCCCAGGAGTTTGAGACCAGTGTGGGCAACAGAGTGAGAACCATCTCTACCCAAAAAAAAAATTAAAAAT
TAGCCAAGCATGGTGATGATATAGGAGTTAAGGAGAAATCATTTAGGCAAATAGCAAGGGTAGGAAGTCCTCAGTAAGGTTTTCCATTTAATGAAAAGCA
GCCCCCAAAATCATTTTCTTTTCTAACAAAGAACAGCCTGTAAAATCGAGCTGCAGACATAGACAAGCAAGCTGGAAGCTTCCACGGGTGAATGCCGGCA
GCTGTGCCAATAGGAAAAAGCTACCTAGACTAGGCATGTCCAAAATGGCGGCTCCAAGTTCCCTTCTCTTTGCCAGCCATGTGTACAGTAAAAAGCAGGC
AACATAGTGTCAGCCAAAGCTCATTTGCATAATAAGATTAGGGTGGGGTGGCCAGCTCACATAGGGGTAGGCCCTAGGTAAATCAGACACCGCCTTCTCA
AGCCTGTCTATAAAATCTGGTACACTATGACGAGGGTCAGATTTCCCATTCAGACGCCCCTCTCCCATGCAAGAGAAAGAGCTGTTCTCCTTTCTCTTTC
TTTTGCCTATTAAACCTCTGCTCCTGGCCAGGCACAGTGGCTCACGCCTATAATCCCAGCACTTTGGGAGGCTGAGGTGGTCAGATCACCTAAGGTCAGG
AGTTCAAGACCAGCCTGGTCAACATGGTGAAATCTTGTCTCTAGTAAAAATACAAAAATATATGAAATCTCACATAGATGATAATATTAAGTTCCAAAAG
CAACTCAACCTGGTAGATTCTAATTTTTTTTGAGGCAGGGTCTTGCTTTGTCACCCATGCTGGAGTACAATGGCACAAACACTGCTCACTGCAGCCTCGA
CCTCCCAAGGCCTAAGCAATCCTCCTGCCTCAGTCCCCCTCCAGGTATTTGAAACTACAGGTGTGTACCACCACACCGGGCTAATTTTTGTATTTTTTGT
AGAGACGTGGGTCTCACTATGCTGCCCAGGCTCAGGTCTTAATCTCCTGAGCTCAGGCAATCCGCAGGCCTCAGCCTCCCTAAGTGCGGGGATTACAGGC
TTGAGCCACTGCACCTAGCCTCTATTTGTTTTACAAAAGAGAAATTGAGATCCTGAATGTTAAGTGACTTGCCTGAGGCCATCCCACTAACAGGAGCCAG
GGTTAGGATTCAAACCCCATCCAACTGGTCCCAGAGCTGGAGCTTCTTGCACTGCCCTACACTACCTACCATCTCCATCCTCTGGGCACCTTTTTATAAG
AACCAAAACATTACAGAGCATTGCTTTGTCAACTCAGCTGGGAACATTTCCCAGTGCAACTCACATTTTTCACTGCTCTGTGCCTGTCCGTATAAGCTCA
ATGAGTATTGATTTAGGGGCTTTGGAGAACTTTGAATGCTACCCCCCAAGTAACCATTGTTGGCAACCTGGTACCTCTACTTTTAGCCATTTCTCCTTCT
CTATAAATAGTGCAGAAGTAACCCACTTGGTAACAGGCATCCTTGCCAAGCCTCCACCACTAGGTCAGTGTAAGAATTAAAGAAAGAGGAAAGAAACACA
AAAAGTGGCTTGATGGTTAAGACAGGTTTATTTTAGAGAAAACACACCTGAGAGGGGCTGCTGGCTGAATTAGGTTAGAGTCTTTTCTACAGACTAAGAG
TGTTTAAGGATTTAGGGTGGGAGAGTTTCTTAGAGGCTTGGACTGCTTCTGTGTTTTTTTTGTTGTGCTTATATGGGAGGGAGAGTGGTGTGTTTGCTTT
TATACATTTTTCTGCAGCTGTAGGCATACCCCCCAAGTCTGCTTTTAGCTTCCCTATTTTAGTGCACCTGGAGGGAAAGGAATGTGCTTATTAAGGCCCA
CTGTTTTACTGGGGCCCATTGTATGAGGGTGAAGTTTGGCAGTTACCCAAGAGACTTTTCCTCCACCTTCCTCTGTGCCCGAGCTGTTTTATCTGCATTT
TACTGTCTGCTTTTTTTGGCTGCTTATAGTTTTTAAAAAAGTAATTTCCTTAAATCCAGAAGGCTAAAAATGAAGCTGAAACTTAAAGTGGCGGTGTTTG
TCCAAAATAACGGGGCTCCTGCTCTGCCAGTCAGTACCCTCAAGTCACTCCTGATCCTCAACCTCCATGCCTAAGGCTGGTTCAAGAGACCACATAATAT
CTGCCTTTTATTACATACATGATGGGTGCATGGGATTCTGCGTGCCCTTTGCTTGATATAGACTGCTAAGGTGAGATGGGGAATATCAGAGTCAGCTGCT
GCTTGAGGAAGCAGAACACACAGCTGGAGGCTTGGAACATGTGGGTCCCTATGAGTGTAGAGCCCATATCCCCATAGAGTCTACCTAGAGCAGGGGTCGC
CAAATGTTTTCTTAAAGAGCCTGATAGTGTATATGTTAGGCTTTGTGAGCCAGGTATTTACAGCAACTCAATTCTACCACTGTGGTATGAAAACAGCTAT
AGACAATCATAAATGAATGATCATGGCTATGTTTTAATAAAACTTTACAGACACTGAACTTGAACTTCCATTGTGATATGAAAACAGCTATAGACAATCA
TAAATGAATGATCATGGCTATGTTTTAATAAAACTTTATGGACACTGAGCTTGAACTTCATATATCAGTCATGTGACACAAAATATCATTCTTCTTTTTA
TTTGTTTTCACCCATTTGAAAAATGTAAAAACTATTCTTAGCTGTACAGAAACAGATGGTGGGTCAGATTTGACTCACTGCCCATAGTTTCCAGACCATG
ATGTTCAGGTTCATGGAAGCACTTCACTCTACACTAATTTATTCATCCATTCATCACATATGTGATGAGCACATACAGACACACCTCAGAGATATTGCAG
GCTCCACTCCAGATCACCACAATAATGTGAATATTGCAATAAAGCAAGTCATACACATT
- refseq_EMR2.F3 (forward primer)
- refseq_EMR2.R3 (reverse primer)
- Alternative acceptor
Frame 0
GGSALCGEASLLGTCLASEEGPFSLPGLLCGHC
NASVFFLSFCF*HRVSLYHPSWSAVAPS*LTAAS
NSPARVILPASAS*VVAAGTTDVCHQAQLHRQF
RGGPWASGCPPPLLYKVLASGQPAQLPSLRRRD
SPVPLTLSPAAPAGSSAGTMGGRVFLVFLAFCVW
LTLPGAETQDSRGCARWCPQDSSCVNATACRCN
PGFSSFSEIITTPMETCDDINECATLSKVSCGK
FSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESEN
TCQDVDECSSGQHQCDSSTVCFNTVGSYSCRCR
PGWKPRHGIPNNQKDTVCEDMTFSTWTPPPGVH
SQTLSRFFDKVQDLGRDYKPGLANNTIQSILQAL
DELLEAPGDLETLPRLQQHCVASHLLDGLEDVL
RGLSKNLSNGLLNFSYPAGTELSLEVQKQVDRS
VTLRQNQAVMQLDWNQAQKSGDPGMGKLLAEAPL
VLEPEKQMLLHETHQGLLQDGSPILLSDVISAF
LSNNDTQNLSSPVTFTFSHRSVIPRQKVLCVFW
EHGQNGCGHWATTGCSTIGTRDTSTICRCTHLSS
FAVLMAHYDVQEEDPVLTVITYMGLSVSLLCLL
LAALTFLLCKAIQNTSTSLHLQLSLCLFLAHLL
FLVAIDQTGHKVLCSIIAGTLHYLYLATLTWMLL
EALYLFLTARNLTVVNYSSINRFMKKLMFPVGY
GVPAVTVAISAASRPHLYGTPSRCWLQPEKGFI
WGFLGPVCAIFSVNLVLFLVTLWILKNRLSSLNS
EVSTLRNTRMLAFKATAQLFILGCTWCLGILQV
GPAARVMAYLFTIINSLQGVFIFLVYCLLSQQV
REQYGKWSKGIRKLKTESEMHTLSSSAKADTSKP
STVN*KNLLNKIFPLCPWKI*TIFEPSRGERKD
FVLCVSRNSPCQQYEGCYGRRARHSIPAETGNL
PCPAMCSSNSQHMDGQLWPISWSL*STIFMKL*N
VKTSFTASPSYKDSSKS*NEAGKRA*EDFHTDN
I*KD*NVHTTIWIS*FFVFVFVVL*FYGFDYLV
M*KILITHT*IKRDTAPAFMELLGENEVALAARV
DSEAEIPRNQVSTARQLKYKLFYKHCLLSSSHQ
HAEKFLISDRGCATNSISKE*PAKLLNIPSKCP
*PCLLSAFLEQKFYMTIKPRRWVWHSSCPVILAL
WEGEAGGWLKPRSLRPVWATE*EPSLPKKKIKN
*PSMVMI*ELRRNHLGK*QG*EVLSKVFHLMKS
SPQNHFLF*QRTACKIELQT*TSKLEASTGECRQ
LCQ*EKAT*TRHVQNGGSKFPSLCQPCVQ*KAG
NIVSAKAHLHNKIRVGWPAHIGVGPR*IRHRLL
KPVYKIWYTMTRVRFPIQTPLSHARERAVLLSLS
FAY*TSAPGQAQWLTPIIPALWEAEVVRSPKVR
SSRPAWSTW*NLVSSKNTKIYEISHR**Y*VPK
ATQPGRF*FFLRQGLALSPMLEYNGTNTAHCSLD
LPRPKQSSCLSPPPGI*NYRCVPPHRANFCIFC
RDVGLTMLPRLRS*SPELRQSAGLSLPKCGDYR
LEPLHLASICFTKEKLRS*MLSDLPEAIPLTGAR
VRIQTPSNWSQSWSFLHCPTLPTISILWAPFYK
NQNITEHCFVNSAGNISQCNSHFSLLCACPYKL
NEY*FRGFGEL*MLPPK*PLLATWYLYF*PFLLL
YK*CRSNPLGNRHPCQASTTRSV*ELKKEERNT
KSGLMVKTGLF*RKHT*EGLLAELG*SLFYRLR
VFKDLGWESFLEAWTASVFFLLCLYGRESGVFAF
IHFSAAVGIPPKSAFSFPILVHLEGKECAY*GP
LFYWGPLYEGEVWQLPKRLFLHLPLCPSCFICI
LLSAFFGCL*FLKK*FP*IQKAKNEAET*SGGVC
PK*RGSCSASQYPQVTPDPQPPCLRLVQETT*Y
LPFITYMMGAWDSACPLLDIDC*GEMGNIRVSC
CLRKQNTQLEAWNMWVPMSVEPISP*SLPRAGVA
KCFLKEPDSVYVRLCEPGIYSNSILPLWYENSY
RQS*MNDHGYVLIKLYRH*T*TSIVI*KQL*TI
INE*SWLCFNKTLWTLSLNFIYQSCDTKYHSSFY
LFSPI*KM*KLFLAVQKQMVGQI*LTAHSFQTM
MFRFMEALHSTLIYSSIHHICDEHIQTHLRDIA
GSTPDHHNNVNIAIKQVIHI
Frame 1
GGLLCAVKLLFLAPAWHRKRAPSPSLGFYVDTV
MPQFSFFLFVFDTGSHSITQAGVQWHHLSSPQP
QTPQLG*FFLPQPPE**QLGPQTSATKPSCTASS
GEGPGPAAVRPPSFIKSWPRDSPHSCPACGDGT
ALSHSLFPLLLLPAAQLEPWEAASFSSFSHSVS
G*LCRELKPRTPGAVPGGALRTPRVSMPPPVAAI
QGSALFLRSSPPPWRLVTTSTSVQHCRKCHAEN
SRTAGTQRGATTACAAQDMSLFLGQKHSRMRAR
TRVKMWTSAAPGSISVTAPPSASTPWVHTAAAAA
QAGSPDTESRITKRTLSVKI*LSPPGPRPLEST
ARRFPDSSTKSRTWAETTSQAWPITPSRASYRR
WMSCWRPLGTWRPCPAYSSTVWPVTCWMA*RMSS
EA*ARTFPMGC*TSVILQAQNCPWRCRSK*TGV
SP*DRIRQ*CSSTGIRHRNLVTQGWASCWLRPL
WSWNLRSRCFCMRHTRACCRTAPPSCSQM*SLPF
*ATTTPKTSAPQLPSPSPTVQ*SRDRRCSVSSG
SMARMDVVTGPPQAAAQ*APETPAPSAVAPT*A
ALPSSWPTTMCRRRIPC*LSSPTWG*ASLCCASS
WRPSLFSCVKPSRTPAPHCICSSRSASSWPTSS
SSWQLIKPDTRCCAPSSPVPCTISTWPP*PGCC
WRPCTSSSLHGT*RWSTTQASTDS*RSSCSLWAT
ESQL*QWPFLQPPGLTFMEHLPAAGSNQKRDLY
GASLDLSAPSSL*I*FSFW*LSGF*KTDSPPSI
VKCPPSGTQGCWHLKRQLSCSSWAARGVWASCRW
VRLPGSWPTSSPSSTACRVSSSSWCTASSASRS
GSNMGNGPKGSGN*KLSLRCTHSPAVLRLTPPN
PARLTRKIF*IRSSLFARGKSEQSLSHLEGKEKT
LFCVFQEIHHVSNMKDVMEGVLGIQFLQKPEIF
HALQCAHQTLSIWTASCGPYLGHSEAQYL*SYR
TLRPLSQPLLPTKTPPNLKMKQENEPKRTFIPTT
SERTRMFTPRSGFLNFLFLFLLFSSSTGLII*S
CEKY*LLTHRSRETRLLPSWSF*GKMKWLLQLE
LTQKPKFLEIRFLLLGN*SINYFINTVFFRLHTN
MQKSF*SQIGDVQQIPFQRNDLQNS*IFQANAL
NPVCYLLSLNRNST*P*NLEDGYGTVHAL*S*H
FGRVRQEDGSSPGV*DQCGQQSENHLYPKKKLKI
SQAW**YRS*GEII*ANSKGRKSSVRFSI**KA
APKIIFFSNKEQPVKSSCRHRQASWKLPRVNAG
SCANRKKLPRLGMSKMAAPSSLLFASHVYSKKQA
T*CQPKLICIIRLGWGGQLT*G*ALGKSDTAFS
SLSIKSGTL*RGSDFPFRRPSPMQEKELFSFLF
LLPIKPLLLARHSGSRL*SQHFGRLRWSDHLRSG
VQDQPGQHGEILSLVKIQKYMKSHIDDNIKFQK
QLNLVDSNFF*GRVLLCHPCWSTMAQTLLTAAS
TSQGLSNPPASVPLQVFETTGVYHHTGLIFVFFV
ETWVSLCCPGSGLNLLSSGNPQASASLSAGITG
LSHCT*PLFVLQKRN*DPEC*VTCLRPSH*QEP
GLGFKPHPTGPRAGASCTALHYLPSPSSGHLFIR
TKTLQSIALSTQLGTFPSATHIFHCSVPVRISS
MSIDLGALENFECYPPSNHCWQPGTSTFSHFSF
SINSAEVTHLVTGILAKPPPLGQCKN*RKRKETQ
KVA*WLRQVYFRENTPERGCWLN*VRVFSTD*E
CLRI*GGRVS*RLGLLLCFFCCAYMGGRVVCLL
LYIFLQL*AYPPSLLLASLF*CTWRERNVLIKAH
CFTGAHCMRVKFGSYPRDFSSTFLCARAVLSAF
YCLLFLAAYSF*KSNFLKSRRLKMKLKLKVAVF
VQNNGAPALPVSTLKSLLILNLHA*GWFKRPHNI
CLLLHT*WVHGILRALCLI*TAKVRWGISESAA
A*GSRTHSWRLGTCGSL*V*SPYPHRVYLEQGS
PNVFLKSLIVYMLGFVSQVFTATQFYHCGMKTAI
DNHK*MIMAMF**NFTDTELELPL*YENSYRQS
*MNDHGYVLIKLYGH*A*TSYISHVTQNIILLF
ICFHPFEKCKNYS*LYRNRWWVRFDSLPIVSRP*
CSGSWKHFTLH*FIHPFITYVMSTYRHTSEILQ
APLQITTIM*ILQ*SKSYT
Frame 2
GVCSVR*SFSSWHLPGIGRGPLLPPWAFMWTL*
CLSFLSFFLFLTQGLTLSPKLECSGTILAHRSL
KLPSSGDSSCLSLLSSSSWDHRRLPPSPAAPPV
PGRALGQRLSAPPPL*SPGLGTARTAAQPAETGQ
PCPTHSFPCCSCRQLSWNHGRPRLSRLSRILCL
ADSAGS*NPGLQGLCPVVPSGLLVCQCHRLSLQ
SRVQLFF*DHHHPHGDL*RHQRVCNTVESVMRKI
LGLLEHRGELRLRVQPRI*ACFWGKNIQE*ERE
HVSRCGRVQLRAASV*QLHRLLQHRGFIQLPLP
PRLEAQTRNPE*PKGHCL*RYDFLHLDPAPWSPQ
PDAFPILRQSPGPGQRLQARLGQ*HHPEHLTGA
G*AAGGPWGPGDPAPLTAALCGQSPAGWPRGCP
QRPEQEPFQWAVELQLSCRHRIVPGGAEASRQEC
HLETESGSDAARLESGTEIW*PRDGQVAG*GPS
GPGT*EADASA*DTPGLAAGRLPHPALRCDLCL
SEQQRHPKPQLPSYLHLLPPFSDPETEGALCLLG
AWPEWMWSLGHHRLQHNRHQRHQHHLPLHPPEQ
LCRPHGPLRCAGGGSRADCHHLHGAERLSAVPP
PGGPHFSPV*SHPEHQHLTASAALALPLPGPPPL
PRGN*SNRTQGAVLHHRRYLALSLPGHLDLDAA
GGPVPLPHCTEPDGGQLLKHQQIHEEAHVPCGL
RSPSCDSGHFCSLQASPLWNTFPLLAPTRKGIYM
GLPWTCLRHLLCEFSSLSGDSLDFEKQTLLPQ*
*SVHPPEHKDAGI*SDSSAVHPGLHVVSGHLAG
GSGCPGHGLPLHHHQQPAGCLHLPGVLPPQPAGP
GAIWEMVQRDQEIEN*V*DAHTLQQC*G*HLQT
QHG*LEKSSE*DLPSLPVENLNNL*AI*RGKKR
LCSVCFKKFTMSAI*RMLWKAC*AFNSCRNRKSS
MPCNVLIKLSAYGRPAVAHILVTLKHNIYEAIE
R*DLFHSLSFLQRLLQILK*SRKTSLRGLSYRQ
HLKGLECSHHDLDFLIFCFCFCCSLVLRV*LFSH
VKNIDYSHIDQERHGSCLHGAFRGK*SGSCS*S
*LRSRNS*KSGFYC*AIEV*TIL*TLSSFVFTP
TCRKVSNLRSGMCNKFHFKGMTCKTPKYSKQMPL
TLSVICFP*TEILHDHKTSKMGMAQFMPCNPST
LGG*GRRMAQAQEFETSVGNRVRTISTQKKN*K
LAKHGDDIGVKEKSFRQIARVGSPQ*GFPFNEKQ
PPKSFSFLTKNSL*NRAADIDKQAGSFHG*MPA
AVPIGKSYLD*ACPKWRLQVPFSLPAMCTVKSR
QHSVSQSSFA**D*GGVASSHRGRP*VNQTPPSQ
ACL*NLVHYDEGQISHSDAPLPCKRKSCSPFSF
FCLLNLCSWPGTVAHAYNPSTLGG*GGQIT*GQ
EFKTSLVNMVKSCL**KYKNI*NLT*MIILSSKS
NSTW*ILIFFEAGSCFVTHAGVQWHKHCSLQPR
PPKA*AILLPQSPSRYLKLQVCTTTPG*FLYFL
*RRGSHYAAQAQVLIS*AQAIRRPQPP*VRGLQA
*ATAPSLYLFYKREIEILNVK*LA*GHPTNRSQ
G*DSNPIQLVPELELLALPYTTYHLHPLGTFL*
EPKHYRALLCQLSWEHFPVQLTFFTALCLSV*AQ
*VLI*GLWRTLNATPQVTIVGNLVPLLLAISPS
L*IVQK*PTW*QASLPSLHH*VSVRIKERGKKH
KKWLDG*DRFILEKTHLRGAAG*IRLESFLQTKS
V*GFRVGEFLRGLDCFCVFFVVLIWEGEWCVCF
YTFFCSCRHTPQVCF*LPYFSAPGGKGMCLLRP
TVLLGPIV*G*SLAVTQETFPPPSSVPELFYLHF
TVCFFWLLIVFKKVISLNPEG*K*S*NLKWRCL
SKITGLLLCQSVPSSHS*SSTSMPKAGSRDHII
SAFYYIHDGCMGFCVPFA*YRLLR*DGEYQSQLL
LEEAEHTAGGLEHVGPYECRAHIPIEST*SRGR
QMFS*RA**CIC*AL*ARYLQQLNSTTVV*KQL
*TIINE*SWLCFNKTLQTLNLNFHCDMKTAIDNH
K*MIMAMF**NFMDTELELHISVM*HKISFFFL
FVFTHLKNVKTILSCTETDGGSDLTHCP*FPDH
DVQVHGSTSLYTNLFIHSSHM**AHTDTPQRYCR
LHSRSPQ*CEYCNKASHTH
Sequence NM_152920
GGGGGGTCTGCTCTGTGCGGTGAAGCTTCTCTTCTTGGCACCTGCCTGGCATCGGAAGAGGGCCCCTTCTCCCTCCCTGGGCTTTTATGTGGACACTGTA
ATGCCTCAGTTTTCTTTCTTTCTTTTTGTTTTTGACACAGGGTCTCACTCTATCACCCAAGCTGGAGTGCAGTGGCACCATCTTAGCTCACCGCAGCCTC
AAACTCCCCAGCTCGGGTGATTCTTCCTGCCTCAGCCTCCTGAGTAGTAGCAGCTGGGACCACAGACGTCTGCCACCAAGCCCAGCTGCACCGCCAGTTC
CGGGGAGGGCCCTGGGCCAGCGGCTGTCCGCCCCCCCTCCTTTATAAAGTCCTGGCCTCGGGACAGCCCGCACAGCTGCCCAGCCTGCGGAGACGGGACA
GCCCTGTCCCACTCACTCTTTCCCCTGCTGCTCCTGCCGGCAGCTCAGCTGGAACCATGGGAGGCCGCGTCTTTCTCGTCTTTCTCGCATTCTGTGTCTG
GCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGGACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAAT
CCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCCATGGAGACTTGTGACGACATCAACGAGTGTGCAACACTGTCGAAAGTGTCATGCGGAAAAT
TCTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCAGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAA
CACGTGTCAAGATGTGGACGAATGTCAGCAGAACCCAAGGCTCTGTAAAAGCTACGGCACCTGCGTCAACACCCTCGGCAGCTACACGTGCCAGTGCCTG
CCTGGCTTCAAGCTCAAACCTGAGGACCCGAAGCTCTGCACAGATGTGGACGAGTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCA
ACACCGTGGGTTCATACAGCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACACTGTCTGTGAAGATATGACTTT
CTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGATTCTTCGACAAAGTCCAGGACCTGGGCAGAGACTACAAGCCAGGCTTGGCC
AATAACACCATCCAGAGCATCTTACAGGCGCTGGATGAGCTGCTGGAGGCCCCTGGGGACCTGGAGACCCTGCCCCGCTTACAGCAGCACTGTGTGGCCA
GTCACCTGCTGGATGGCCTAGAGGATGTCCTCAGAGGCCTGAGCAAGAACCTTTCCAATGGGCTGTTGAACTTCAGTTATCCTGCAGGCACAGAATTGTC
CCTGGAGGTGCAGAAGCAAGTAGACAGGAGTGTCACCTTGAGACAGAATCAGGCAGTGATGCAGCTCGACTGGAATCAGGCACAGAAATCTGGTGACCCA
GGGATGGGCAAGTTGCTGGCTGAGGCCCCTCTGGTCCTGGAACCTGAGAAGCAGATGCTTCTGCATGAGACACACCAGGGCTTGCTGCAGGACGGCTCCC
CCATCCTGCTCTCAGATGTGATCTCTGCCTTTCTGAGCAACAACGACACCCAAAACCTCAGCTCCCCAGTTACCTTCACCTTCTCCCACCGTTCAGTGAT
CCCGAGACAGAAGGTGCTCTGTGTCTTCTGGGAGCATGGCCAGAATGGATGTGGTCACTGGGCCACCACAGGCTGCAGCACAATAGGCACCAGAGACACC
AGCACCATCTGCCGTTGCACCCACCTGAGCAGCTTTGCCGTCCTCATGGCCCACTACGATGTGCAGGAGGAGGATCCCGTGCTGACTGTCATCACCTACA
TGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTCCTGGCGGCCCTCACTTTTCTCCTGTGTAAAGCCATCCAGAACACCAGCACCTCACTGCATCTGCAGCT
CTCGCTCTGCCTCTTCCTGGCCCACCTCCTCTTCCTCGTGGCAATTGATCAAACCGGACACAAGGTGCTGTGCTCCATCATCGCCGGTACCTTGCACTAT
CTCTACCTGGCCACCTTGACCTGGATGCTGCTGGAGGCCCTGTACCTCTTCCTCACTGCACGGAACCTGACGGTGGTCAACTACTCAAGCATCAACAGAT
TCATGAAGAAGCTCATGTTCCCTGTGGGCTACGGAGTCCCAGCTGTGACAGTGGCCATTTCTGCAGCCTCCAGGCCTCACCTTTATGGAACACCTTCCCG
CTGCTGGCTCCAACCAGAAAAGGGATTTATATGGGGCTTCCTTGGACCTGTCTGCGCCATCTTCTCTGTGAATTTAGTTCTCTTTCTGGTGACTCTCTGG
ATTTTGAAAAACAGACTCTCCTCCCTCAATAGTGAAGTGTCCACCCTCCGGAACACAAGGATGCTGGCATTTAAAGCGACAGCTCAGCTGTTCATCCTGG
GCTGCACGTGGTGTCTGGGCATCTTGCAGGTGGGTCCGGCTGCCCGGGTCATGGCCTACCTCTTCACCATCATCAACAGCCTGCAGGGTGTCTTCATCTT
CCTGGTGTACTGCCTCCTCAGCCAGCAGGTCCGGGAGCAATATGGGAAATGGTCCAAAGGGATCAGGAAATTGAAAACTGAGTCTGAGATGCACACACTC
TCCAGCAGTGCTAAGGCTGACACCTCCAAACCCAGCACGGTTAACTAGAAAAATCTTCTGAATAAGATCTTCCCTCTTTGCCCGTGGAAAATCTGAACAA
TCTTTGAGCCATCTAGAGGGGAAAGAAAAGACTTTGTTCTGTGTGTTTCAAGAAATTCACCATGTCAGCAATATGAAGGATGTTATGGAAGGCGTGCTAG
GCATTCAATTCCTGCAGAAACCGGAAATCTTCCATGCCCTGCAATGTGCTCATCAAACTCTCAGCATATGGACGGCCAGCTGTGGCCCATATCTTGGTCA
CTCTGAAGCACAATATTTATGAAGCTATAGAACGTTAAGACCTCTTTCACAGCCTCTCCTTCCTACAAAGACTCCTCCAAATCTTAAAATGAAGCAGGAA
AACGAGCCTAAGAGGACTTTCATACCGACAACATCTGAAAGGACTAGAATGTTCACACCACGATCTGGATTTCTTAATTTTTTGTTTTTGTTTTTGTTGT
TCTCTAGTTCTACGGGTTTGATTATTTAGTCATGTGAAAAATATTGATTACTCACACATAGATCAAGAGAGACACGGCTCCTGCCTTCATGGAGCTTTTA
GGGGAAAATGAAGTGGCTCTTGCAGCTAGAGTTGACTCAGAAGCCGAAATTCCTAGAAATCAGGTTTCTACTGCTAGGCAATTGAAGTATAAACTATTTT
ATAAACACTGTCTTCTTTCGTCTTCACACCAACATGCAGAAAAGTTTCTAATCTCAGATCGGGGATGTGCAACAAATTCCATTTCAAAGGAATGACCTGC
AAAACTCCTAAATATTCCAAGCAAATGCCCTTAACCCTGTCTGTTATCTGCTTTCCTTGAACAGAAATTCTACATGACCATAAAACCTCGAAGATGGGTA
TGGCACAGTTCATGCCCTGTAATCCTAGCACTTTGGGAGGGTGAGGCAGGAGGATGGCTCAAGCCCAGGAGTTTGAGACCAGTGTGGGCAACAGAGTGAG
AACCATCTCTACCCAAAAAAAAAATTAAAAATTAGCCAAGCATGGTGATGATATAGGAGTTAAGGAGAAATCATTTAGGCAAATAGCAAGGGTAGGAAGT
CCTCAGTAAGGTTTTCCATTTAATGAAAAGCAGCCCCCAAAATCATTTTCTTTTCTAACAAAGAACAGCCTGTAAAATCGAGCTGCAGACATAGACAAGC
AAGCTGGAAGCTTCCACGGGTGAATGCCGGCAGCTGTGCCAATAGGAAAAAGCTACCTAGACTAGGCATGTCCAAAATGGCGGCTCCAAGTTCCCTTCTC
TTTGCCAGCCATGTGTACAGTAAAAAGCAGGCAACATAGTGTCAGCCAAAGCTCATTTGCATAATAAGATTAGGGTGGGGTGGCCAGCTCACATAGGGGT
AGGCCCTAGGTAAATCAGACACCGCCTTCTCAAGCCTGTCTATAAAATCTGGTACACTATGACGAGGGTCAGATTTCCCATTCAGACGCCCCTCTCCCAT
GCAAGAGAAAGAGCTGTTCTCCTTTCTCTTTCTTTTGCCTATTAAACCTCTGCTCCTGGCCAGGCACAGTGGCTCACGCCTATAATCCCAGCACTTTGGG
AGGCTGAGGTGGTCAGATCACCTAAGGTCAGGAGTTCAAGACCAGCCTGGTCAACATGGTGAAATCTTGTCTCTAGTAAAAATACAAAAATATATGAAAT
CTCACATAGATGATAATATTAAGTTCCAAAAGCAACTCAACCTGGTAGATTCTAATTTTTTTTGAGGCAGGGTCTTGCTTTGTCACCCATGCTGGAGTAC
AATGGCACAAACACTGCTCACTGCAGCCTCGACCTCCCAAGGCCTAAGCAATCCTCCTGCCTCAGTCCCCCTCCAGGTATTTGAAACTACAGGTGTGTAC
CACCACACCGGGCTAATTTTTGTATTTTTTGTAGAGACGTGGGTCTCACTATGCTGCCCAGGCTCAGGTCTTAATCTCCTGAGCTCAGGCAATCCGCAGG
CCTCAGCCTCCCTAAGTGCGGGGATTACAGGCTTGAGCCACTGCACCTAGCCTCTATTTGTTTTACAAAAGAGAAATTGAGATCCTGAATGTTAAGTGAC
TTGCCTGAGGCCATCCCACTAACAGGAGCCAGGGTTAGGATTCAAACCCCATCCAACTGGTCCCAGAGCTGGAGCTTCTTGCACTGCCCTACACTACCTA
CCATCTCCATCCTCTGGGCACCTTTTTATAAGAACCAAAACATTACAGAGCATTGCTTTGTCAACTCAGCTGGGAACATTTCCCAGTGCAACTCACATTT
TTCACTGCTCTGTGCCTGTCCGTATAAGCTCAATGAGTATTGATTTAGGGGCTTTGGAGAACTTTGAATGCTACCCCCCAAGTAACCATTGTTGGCAACC
TGGTACCTCTACTTTTAGCCATTTCTCCTTCTCTATAAATAGTGCAGAAGTAACCCACTTGGTAACAGGCATCCTTGCCAAGCCTCCACCACTAGGTCAG
TGTAAGAATTAAAGAAAGAGGAAAGAAACACAAAAAGTGGCTTGATGGTTAAGACAGGTTTATTTTAGAGAAAACACACCTGAGAGGGGCTGCTGGCTGA
ATTAGGTTAGAGTCTTTTCTACAGACTAAGAGTGTTTAAGGATTTAGGGTGGGAGAGTTTCTTAGAGGCTTGGACTGCTTCTGTGTTTTTTTTGTTGTGC
TTATATGGGAGGGAGAGTGGTGTGTTTGCTTTTATACATTTTTCTGCAGCTGTAGGCATACCCCCCAAGTCTGCTTTTAGCTTCCCTATTTTAGTGCACC
TGGAGGGAAAGGAATGTGCTTATTAAGGCCCACTGTTTTACTGGGGCCCATTGTATGAGGGTGAAGTTTGGCAGTTACCCAAGAGACTTTTCCTCCACCT
TCCTCTGTGCCCGAGCTGTTTTATCTGCATTTTACTGTCTGCTTTTTTTGGCTGCTTATAGTTTTTAAAAAAGTAATTTCCTTAAATCCAGAAGGCTAAA
AATGAAGCTGAAACTTAAAGTGGCGGTGTTTGTCCAAAATAACGGGGCTCCTGCTCTGCCAGTCAGTACCCTCAAGTCACTCCTGATCCTCAACCTCCAT
GCCTAAGGCTGGTTCAAGAGACCACATAATATCTGCCTTTTATTACATACATGATGGGTGCATGGGATTCTGCGTGCCCTTTGCTTGATATAGACTGCTA
AGGTGAGATGGGGAATATCAGAGTCAGCTGCTGCTTGAGGAAGCAGAACACACAGCTGGAGGCTTGGAACATGTGGGTCCCTATGAGTGTAGAGCCCATA
TCCCCATAGAGTCTACCTAGAGCAGGGGTCGCCAAATGTTTTCTTAAAGAGCCTGATAGTGTATATGTTAGGCTTTGTGAGCCAGGTATTTACAGCAACT
CAATTCTACCACTGTGGTATGAAAACAGCTATAGACAATCATAAATGAATGATCATGGCTATGTTTTAATAAAACTTTACAGACACTGAACTTGAACTTC
CATTGTGATATGAAAACAGCTATAGACAATCATAAATGAATGATCATGGCTATGTTTTAATAAAACTTTATGGACACTGAGCTTGAACTTCATATATCAG
TCATGTGACACAAAATATCATTCTTCTTTTTATTTGTTTTCACCCATTTGAAAAATGTAAAAACTATTCTTAGCTGTACAGAAACAGATGGTGGGTCAGA
TTTGACTCACTGCCCATAGTTTCCAGACCATGATGTTCAGGTTCATGGAAGCACTTCACTCTACACTAATTTATTCATCCATTCATCACATATGTGATGA
GCACATACAGACACACCTCAGAGATATTGCAGGCTCCACTCCAGATCACCACAATAATGTGAATATTGCAATAAAGCAAGTCATACACATT
- refseq_EMR2.F3 (forward primer)
- refseq_EMR2.R3 (reverse primer)
- Alternative acceptor
Frame 0
GGSALCGEASLLGTCLASEEGPFSLPGLLCGHC
NASVFFLSFCF*HRVSLYHPSWSAVAPS*LTAAS
NSPARVILPASAS*VVAAGTTDVCHQAQLHRQF
RGGPWASGCPPPLLYKVLASGQPAQLPSLRRRD
SPVPLTLSPAAPAGSSAGTMGGRVFLVFLAFCVW
LTLPGAETQDSRGCARWCPQDSSCVNATACRCN
PGFSSFSEIITTPMETCDDINECATLSKVSCGK
FSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESEN
TCQDVDECQQNPRLCKSYGTCVNTLGSYTCQCL
PGFKLKPEDPKLCTDVDECSSGQHQCDSSTVCF
NTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMTF
STWTPPPGVHSQTLSRFFDKVQDLGRDYKPGLA
NNTIQSILQALDELLEAPGDLETLPRLQQHCVA
SHLLDGLEDVLRGLSKNLSNGLLNFSYPAGTELS
LEVQKQVDRSVTLRQNQAVMQLDWNQAQKSGDP
GMGKLLAEAPLVLEPEKQMLLHETHQGLLQDGS
PILLSDVISAFLSNNDTQNLSSPVTFTFSHRSVI
PRQKVLCVFWEHGQNGCGHWATTGCSTIGTRDT
STICRCTHLSSFAVLMAHYDVQEEDPVLTVITY
MGLSVSLLCLLLAALTFLLCKAIQNTSTSLHLQL
SLCLFLAHLLFLVAIDQTGHKVLCSIIAGTLHY
LYLATLTWMLLEALYLFLTARNLTVVNYSSINR
FMKKLMFPVGYGVPAVTVAISAASRPHLYGTPSR
CWLQPEKGFIWGFLGPVCAIFSVNLVLFLVTLW
ILKNRLSSLNSEVSTLRNTRMLAFKATAQLFIL
GCTWCLGILQVGPAARVMAYLFTIINSLQGVFIF
LVYCLLSQQVREQYGKWSKGIRKLKTESEMHTL
SSSAKADTSKPSTVN*KNLLNKIFPLCPWKI*T
IFEPSRGERKDFVLCVSRNSPCQQYEGCYGRRAR
HSIPAETGNLPCPAMCSSNSQHMDGQLWPISWS
L*STIFMKL*NVKTSFTASPSYKDSSKS*NEAG
KRA*EDFHTDNI*KD*NVHTTIWIS*FFVFVFVV
L*FYGFDYLVM*KILITHT*IKRDTAPAFMELL
GENEVALAARVDSEAEIPRNQVSTARQLKYKLF
YKHCLLSSSHQHAEKFLISDRGCATNSISKE*PA
KLLNIPSKCP*PCLLSAFLEQKFYMTIKPRRWV
WHSSCPVILALWEGEAGGWLKPRSLRPVWATE*
EPSLPKKKIKN*PSMVMI*ELRRNHLGK*QG*EV
LSKVFHLMKSSPQNHFLF*QRTACKIELQT*TS
KLEASTGECRQLCQ*EKAT*TRHVQNGGSKFPS
LCQPCVQ*KAGNIVSAKAHLHNKIRVGWPAHIGV
GPR*IRHRLLKPVYKIWYTMTRVRFPIQTPLSH
ARERAVLLSLSFAY*TSAPGQAQWLTPIIPALW
EAEVVRSPKVRSSRPAWSTW*NLVSSKNTKIYEI
SHR**Y*VPKATQPGRF*FFLRQGLALSPMLEY
NGTNTAHCSLDLPRPKQSSCLSPPPGI*NYRCV
PPHRANFCIFCRDVGLTMLPRLRS*SPELRQSAG
LSLPKCGDYRLEPLHLASICFTKEKLRS*MLSD
LPEAIPLTGARVRIQTPSNWSQSWSFLHCPTLP
TISILWAPFYKNQNITEHCFVNSAGNISQCNSHF
SLLCACPYKLNEY*FRGFGEL*MLPPK*PLLAT
WYLYF*PFLLLYK*CRSNPLGNRHPCQASTTRS
V*ELKKEERNTKSGLMVKTGLF*RKHT*EGLLAE
LG*SLFYRLRVFKDLGWESFLEAWTASVFFLLC
LYGRESGVFAFIHFSAAVGIPPKSAFSFPILVH
LEGKECAY*GPLFYWGPLYEGEVWQLPKRLFLHL
PLCPSCFICILLSAFFGCL*FLKK*FP*IQKAK
NEAET*SGGVCPK*RGSCSASQYPQVTPDPQPP
CLRLVQETT*YLPFITYMMGAWDSACPLLDIDC*
GEMGNIRVSCCLRKQNTQLEAWNMWVPMSVEPI
SP*SLPRAGVAKCFLKEPDSVYVRLCEPGIYSN
SILPLWYENSYRQS*MNDHGYVLIKLYRH*T*TS
IVI*KQL*TIINE*SWLCFNKTLWTLSLNFIYQ
SCDTKYHSSFYLFSPI*KM*KLFLAVQKQMVGQ
I*LTAHSFQTMMFRFMEALHSTLIYSSIHHICDE
HIQTHLRDIAGSTPDHHNNVNIAIKQVIHI
Frame 1
GGLLCAVKLLFLAPAWHRKRAPSPSLGFYVDTV
MPQFSFFLFVFDTGSHSITQAGVQWHHLSSPQP
QTPQLG*FFLPQPPE**QLGPQTSATKPSCTASS
GEGPGPAAVRPPSFIKSWPRDSPHSCPACGDGT
ALSHSLFPLLLLPAAQLEPWEAASFSSFSHSVS
G*LCRELKPRTPGAVPGGALRTPRVSMPPPVAAI
QGSALFLRSSPPPWRLVTTSTSVQHCRKCHAEN
SRTAGTQRGATTACAAQDMSLFLGQKHSRMRAR
TRVKMWTNVSRTQGSVKATAPASTPSAATRASAC
LASSSNLRTRSSAQMWTSAAPGSISVTAPPSAS
TPWVHTAAAAAQAGSPDTESRITKRTLSVKI*L
SPPGPRPLESTARRFPDSSTKSRTWAETTSQAWP
ITPSRASYRRWMSCWRPLGTWRPCPAYSSTVWP
VTCWMA*RMSSEA*ARTFPMGC*TSVILQAQNC
PWRCRSK*TGVSP*DRIRQ*CSSTGIRHRNLVTQ
GWASCWLRPLWSWNLRSRCFCMRHTRACCRTAP
PSCSQM*SLPF*ATTTPKTSAPQLPSPSPTVQ*
SRDRRCSVSSGSMARMDVVTGPPQAAAQ*APETP
APSAVAPT*AALPSSWPTTMCRRRIPC*LSSPT
WG*ASLCCASSWRPSLFSCVKPSRTPAPHCICS
SRSASSWPTSSSSWQLIKPDTRCCAPSSPVPCTI
STWPP*PGCCWRPCTSSSLHGT*RWSTTQASTD
S*RSSCSLWATESQL*QWPFLQPPGLTFMEHLP
AAGSNQKRDLYGASLDLSAPSSL*I*FSFW*LSG
F*KTDSPPSIVKCPPSGTQGCWHLKRQLSCSSW
AARGVWASCRWVRLPGSWPTSSPSSTACRVSSS
SWCTASSASRSGSNMGNGPKGSGN*KLSLRCTHS
PAVLRLTPPNPARLTRKIF*IRSSLFARGKSEQ
SLSHLEGKEKTLFCVFQEIHHVSNMKDVMEGVL
GIQFLQKPEIFHALQCAHQTLSIWTASCGPYLGH
SEAQYL*SYRTLRPLSQPLLPTKTPPNLKMKQE
NEPKRTFIPTTSERTRMFTPRSGFLNFLFLFLL
FSSSTGLII*SCEKY*LLTHRSRETRLLPSWSF*
GKMKWLLQLELTQKPKFLEIRFLLLGN*SINYF
INTVFFRLHTNMQKSF*SQIGDVQQIPFQRNDL
QNS*IFQANALNPVCYLLSLNRNST*P*NLEDGY
GTVHAL*S*HFGRVRQEDGSSPGV*DQCGQQSE
NHLYPKKKLKISQAW**YRS*GEII*ANSKGRK
SSVRFSI**KAAPKIIFFSNKEQPVKSSCRHRQA
SWKLPRVNAGSCANRKKLPRLGMSKMAAPSSLL
FASHVYSKKQAT*CQPKLICIIRLGWGGQLT*G
*ALGKSDTAFSSLSIKSGTL*RGSDFPFRRPSPM
QEKELFSFLFLLPIKPLLLARHSGSRL*SQHFG
RLRWSDHLRSGVQDQPGQHGEILSLVKIQKYMK
SHIDDNIKFQKQLNLVDSNFF*GRVLLCHPCWST
MAQTLLTAASTSQGLSNPPASVPLQVFETTGVY
HHTGLIFVFFVETWVSLCCPGSGLNLLSSGNPQ
ASASLSAGITGLSHCT*PLFVLQKRN*DPEC*VT
CLRPSH*QEPGLGFKPHPTGPRAGASCTALHYL
PSPSSGHLFIRTKTLQSIALSTQLGTFPSATHI
FHCSVPVRISSMSIDLGALENFECYPPSNHCWQP
GTSTFSHFSFSINSAEVTHLVTGILAKPPPLGQ
CKN*RKRKETQKVA*WLRQVYFRENTPERGCWL
N*VRVFSTD*ECLRI*GGRVS*RLGLLLCFFCCA
YMGGRVVCLLLYIFLQL*AYPPSLLLASLF*CT
WRERNVLIKAHCFTGAHCMRVKFGSYPRDFSST
FLCARAVLSAFYCLLFLAAYSF*KSNFLKSRRLK
MKLKLKVAVFVQNNGAPALPVSTLKSLLILNLH
A*GWFKRPHNICLLLHT*WVHGILRALCLI*TA
KVRWGISESAAA*GSRTHSWRLGTCGSL*V*SPY
PHRVYLEQGSPNVFLKSLIVYMLGFVSQVFTAT
QFYHCGMKTAIDNHK*MIMAMF**NFTDTELEL
PL*YENSYRQS*MNDHGYVLIKLYGH*A*TSYIS
HVTQNIILLFICFHPFEKCKNYS*LYRNRWWVR
FDSLPIVSRP*CSGSWKHFTLH*FIHPFITYVM
STYRHTSEILQAPLQITTIM*ILQ*SKSYT
Frame 2
GVCSVR*SFSSWHLPGIGRGPLLPPWAFMWTL*
CLSFLSFFLFLTQGLTLSPKLECSGTILAHRSL
KLPSSGDSSCLSLLSSSSWDHRRLPPSPAAPPV
PGRALGQRLSAPPPL*SPGLGTARTAAQPAETGQ
PCPTHSFPCCSCRQLSWNHGRPRLSRLSRILCL
ADSAGS*NPGLQGLCPVVPSGLLVCQCHRLSLQ
SRVQLFF*DHHHPHGDL*RHQRVCNTVESVMRKI
LGLLEHRGELRLRVQPRI*ACFWGKNIQE*ERE
HVSRCGRMSAEPKAL*KLRHLRQHPRQLHVPVP
AWLQAQT*GPEALHRCGRVQLRAASV*QLHRLLQ
HRGFIQLPLPPRLEAQTRNPE*PKGHCL*RYDF
LHLDPAPWSPQPDAFPILRQSPGPGQRLQARLG
Q*HHPEHLTGAG*AAGGPWGPGDPAPLTAALCGQ
SPAGWPRGCPQRPEQEPFQWAVELQLSCRHRIV
PGGAEASRQECHLETESGSDAARLESGTEIW*P
RDGQVAG*GPSGPGT*EADASA*DTPGLAAGRLP
HPALRCDLCLSEQQRHPKPQLPSYLHLLPPFSD
PETEGALCLLGAWPEWMWSLGHHRLQHNRHQRH
QHHLPLHPPEQLCRPHGPLRCAGGGSRADCHHLH
GAERLSAVPPPGGPHFSPV*SHPEHQHLTASAA
LALPLPGPPPLPRGN*SNRTQGAVLHHRRYLAL
SLPGHLDLDAAGGPVPLPHCTEPDGGQLLKHQQI
HEEAHVPCGLRSPSCDSGHFCSLQASPLWNTFP
LLAPTRKGIYMGLPWTCLRHLLCEFSSLSGDSL
DFEKQTLLPQ**SVHPPEHKDAGI*SDSSAVHPG
LHVVSGHLAGGSGCPGHGLPLHHHQQPAGCLHL
PGVLPPQPAGPGAIWEMVQRDQEIEN*V*DAHT
LQQC*G*HLQTQHG*LEKSSE*DLPSLPVENLNN
L*AI*RGKKRLCSVCFKKFTMSAI*RMLWKAC*
AFNSCRNRKSSMPCNVLIKLSAYGRPAVAHILV
TLKHNIYEAIER*DLFHSLSFLQRLLQILK*SRK
TSLRGLSYRQHLKGLECSHHDLDFLIFCFCFCC
SLVLRV*LFSHVKNIDYSHIDQERHGSCLHGAF
RGK*SGSCS*S*LRSRNS*KSGFYC*AIEV*TIL
*TLSSFVFTPTCRKVSNLRSGMCNKFHFKGMTC
KTPKYSKQMPLTLSVICFP*TEILHDHKTSKMG
MAQFMPCNPSTLGG*GRRMAQAQEFETSVGNRVR
TISTQKKN*KLAKHGDDIGVKEKSFRQIARVGS
PQ*GFPFNEKQPPKSFSFLTKNSL*NRAADIDK
QAGSFHG*MPAAVPIGKSYLD*ACPKWRLQVPFS
LPAMCTVKSRQHSVSQSSFA**D*GGVASSHRG
RP*VNQTPPSQACL*NLVHYDEGQISHSDAPLP
CKRKSCSPFSFFCLLNLCSWPGTVAHAYNPSTLG
G*GGQIT*GQEFKTSLVNMVKSCL**KYKNI*N
LT*MIILSSKSNSTW*ILIFFEAGSCFVTHAGV
QWHKHCSLQPRPPKA*AILLPQSPSRYLKLQVCT
TTPG*FLYFL*RRGSHYAAQAQVLIS*AQAIRR
PQPP*VRGLQA*ATAPSLYLFYKREIEILNVK*
LA*GHPTNRSQG*DSNPIQLVPELELLALPYTTY
HLHPLGTFL*EPKHYRALLCQLSWEHFPVQLTF
FTALCLSV*AQ*VLI*GLWRTLNATPQVTIVGN
LVPLLLAISPSL*IVQK*PTW*QASLPSLHH*VS
VRIKERGKKHKKWLDG*DRFILEKTHLRGAAG*
IRLESFLQTKSV*GFRVGEFLRGLDCFCVFFVV
LIWEGEWCVCFYTFFCSCRHTPQVCF*LPYFSAP
GGKGMCLLRPTVLLGPIV*G*SLAVTQETFPPP
SSVPELFYLHFTVCFFWLLIVFKKVISLNPEG*
K*S*NLKWRCLSKITGLLLCQSVPSSHS*SSTSM
PKAGSRDHIISAFYYIHDGCMGFCVPFA*YRLL
R*DGEYQSQLLLEEAEHTAGGLEHVGPYECRAH
IPIEST*SRGRQMFS*RA**CIC*AL*ARYLQQL
NSTTVV*KQL*TIINE*SWLCFNKTLQTLNLNF
HCDMKTAIDNHK*MIMAMF**NFMDTELELHIS
VM*HKISFFFLFVFTHLKNVKTILSCTETDGGSD
LTHCP*FPDHDVQVHGSTSLYTNLFIHSSHM**
AHTDTPQRYCRLHSRSPQ*CEYCNKASHTH
Sequence NM_013447
GGGGGGTCTGCTCTGTGCGGTGAAGCTTCTCTTCTTGGCACCTGCCTGGCATCGGAAGAGGGCCCCTTCTCCCTCCCTGGGCTTTTATGTGGACACTGTA
ATGCCTCAGTTTTCTTTCTTTCTTTTTGTTTTTGACACAGGGTCTCACTCTATCACCCAAGCTGGAGTGCAGTGGCACCATCTTAGCTCACCGCAGCCTC
AAACTCCCCAGCTCGGGTGATTCTTCCTGCCTCAGCCTCCTGAGTAGTAGCAGCTGGGACCACAGACGTCTGCCACCAAGCCCAGCTGCACCGCCAGTTC
CGGGGAGGGCCCTGGGCCAGCGGCTGTCCGCCCCCCCTCCTTTATAAAGTCCTGGCCTCGGGACAGCCCGCACAGCTGCCCAGCCTGCGGAGACGGGACA
GCCCTGTCCCACTCACTCTTTCCCCTGCTGCTCCTGCCGGCAGCTCAGCTGGAACCATGGGAGGCCGCGTCTTTCTCGTCTTTCTCGCATTCTGTGTCTG
GCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGGACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAAT
CCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCCATGGAGACTTGTGACGACATCAACGAGTGTGCAACACTGTCGAAAGTGTCATGCGGAAAAT
TCTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCAGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAA
CACGTGTCAAGATGTGGACGAATGTCAGCAGAACCCAAGGCTCTGTAAAAGCTACGGCACCTGCGTCAACACCCTCGGCAGCTACACGTGCCAGTGCCTG
CCTGGCTTCAAGCTCAAACCTGAGGACCCGAAGCTCTGCACAGATGTGAATGAATGCACCTCCGGACAAAACCCATGCCACAGCTCCACCCACTGCCTCA
ACAACGTGGGCAGCTATCAGTGCCGCTGCCGCCCGGGCTGGCAACCGATTCCGGGGTCCCCCAATGGCCCAAACAATACCGTCTGTGAAGATGTGGACGA
GTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATACAGCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACAC
GGAATCCCGAATAACCAAAAGGACACTGTCTGTGAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGATTCT
TCGACAAAGTCCAGGACCTGGGCAGAGACTACAAGCCAGGCTTGGCCAATAACACCATCCAGAGCATCTTACAGGCGCTGGATGAGCTGCTGGAGGCCCC
TGGGGACCTGGAGACCCTGCCCCGCTTACAGCAGCACTGTGTGGCCAGTCACCTGCTGGATGGCCTAGAGGATGTCCTCAGAGGCCTGAGCAAGAACCTT
TCCAATGGGCTGTTGAACTTCAGTTATCCTGCAGGCACAGAATTGTCCCTGGAGGTGCAGAAGCAAGTAGACAGGAGTGTCACCTTGAGACAGAATCAGG
CAGTGATGCAGCTCGACTGGAATCAGGCACAGAAATCTGGTGACCCAGGCCCTTCTGTGGTGGGCCTTGTCTCCATTCCAGGGATGGGCAAGTTGCTGGC
TGAGGCCCCTCTGGTCCTGGAACCTGAGAAGCAGATGCTTCTGCATGAGACACACCAGGGCTTGCTGCAGGACGGCTCCCCCATCCTGCTCTCAGATGTG
ATCTCTGCCTTTCTGAGCAACAACGACACCCAAAACCTCAGCTCCCCAGTTACCTTCACCTTCTCCCACCGTTCAGTGATCCCGAGACAGAAGGTGCTCT
GTGTCTTCTGGGAGCATGGCCAGAATGGATGTGGTCACTGGGCCACCACAGGCTGCAGCACAATAGGCACCAGAGACACCAGCACCATCTGCCGTTGCAC
CCACCTGAGCAGCTTTGCCGTCCTCATGGCCCACTACGATGTGCAGGAGGAGGATCCCGTGCTGACTGTCATCACCTACATGGGGCTGAGCGTCTCTCTG
CTGTGCCTCCTCCTGGCGGCCCTCACTTTTCTCCTGTGTAAAGCCATCCAGAACACCAGCACCTCACTGCATCTGCAGCTCTCGCTCTGCCTCTTCCTGG
CCCACCTCCTCTTCCTCGTGGCAATTGATCAAACCGGACACAAGGTGCTGTGCTCCATCATCGCCGGTACCTTGCACTATCTCTACCTGGCCACCTTGAC
CTGGATGCTGCTGGAGGCCCTGTACCTCTTCCTCACTGCACGGAACCTGACGGTGGTCAACTACTCAAGCATCAACAGATTCATGAAGAAGCTCATGTTC
CCTGTGGGCTACGGAGTCCCAGCTGTGACAGTGGCCATTTCTGCAGCCTCCAGGCCTCACCTTTATGGAACACCTTCCCGCTGCTGGCTCCAACCAGAAA
AGGGATTTATATGGGGCTTCCTTGGACCTGTCTGCGCCATCTTCTCTGTGAATTTAGTTCTCTTTCTGGTGACTCTCTGGATTTTGAAAAACAGACTCTC
CTCCCTCAATAGTGAAGTGTCCACCCTCCGGAACACAAGGATGCTGGCATTTAAAGCGACAGCTCAGCTGTTCATCCTGGGCTGCACGTGGTGTCTGGGC
ATCTTGCAGGTGGGTCCGGCTGCCCGGGTCATGGCCTACCTCTTCACCATCATCAACAGCCTGCAGGGTGTCTTCATCTTCCTGGTGTACTGCCTCCTCA
GCCAGCAGGTCCGGGAGCAATATGGGAAATGGTCCAAAGGGATCAGGAAATTGAAAACTGAGTCTGAGATGCACACACTCTCCAGCAGTGCTAAGGCTGA
CACCTCCAAACCCAGCACGGTTAACTAGAAAAATCTTCTGAATAAGATCTTCCCTCTTTGCCCGTGGAAAATCTGAACAATCTTTGAGCCATCTAGAGGG
GAAAGAAAAGACTTTGTTCTGTGTGTTTCAAGAAATTCACCATGTCAGCAATATGAAGGATGTTATGGAAGGCGTGCTAGGCATTCAATTCCTGCAGAAA
CCGGAAATCTTCCATGCCCTGCAATGTGCTCATCAAACTCTCAGCATATGGACGGCCAGCTGTGGCCCATATCTTGGTCACTCTGAAGCACAATATTTAT
GAAGCTATAGAACGTTAAGACCTCTTTCACAGCCTCTCCTTCCTACAAAGACTCCTCCAAATCTTAAAATGAAGCAGGAAAACGAGCCTAAGAGGACTTT
CATACCGACAACATCTGAAAGGACTAGAATGTTCACACCACGATCTGGATTTCTTAATTTTTTGTTTTTGTTTTTGTTGTTCTCTAGTTCTACGGGTTTG
ATTATTTAGTCATGTGAAAAATATTGATTACTCACACATAGATCAAGAGAGACACGGCTCCTGCCTTCATGGAGCTTTTAGGGGAAAATGAAGTGGCTCT
TGCAGCTAGAGTTGACTCAGAAGCCGAAATTCCTAGAAATCAGGTTTCTACTGCTAGGCAATTGAAGTATAAACTATTTTATAAACACTGTCTTCTTTCG
TCTTCACACCAACATGCAGAAAAGTTTCTAATCTCAGATCGGGGATGTGCAACAAATTCCATTTCAAAGGAATGACCTGCAAAACTCCTAAATATTCCAA
GCAAATGCCCTTAACCCTGTCTGTTATCTGCTTTCCTTGAACAGAAATTCTACATGACCATAAAACCTCGAAGATGGGTATGGCACAGTTCATGCCCTGT
AATCCTAGCACTTTGGGAGGGTGAGGCAGGAGGATGGCTCAAGCCCAGGAGTTTGAGACCAGTGTGGGCAACAGAGTGAGAACCATCTCTACCCAAAAAA
AAAATTAAAAATTAGCCAAGCATGGTGATGATATAGGAGTTAAGGAGAAATCATTTAGGCAAATAGCAAGGGTAGGAAGTCCTCAGTAAGGTTTTCCATT
TAATGAAAAGCAGCCCCCAAAATCATTTTCTTTTCTAACAAAGAACAGCCTGTAAAATCGAGCTGCAGACATAGACAAGCAAGCTGGAAGCTTCCACGGG
TGAATGCCGGCAGCTGTGCCAATAGGAAAAAGCTACCTAGACTAGGCATGTCCAAAATGGCGGCTCCAAGTTCCCTTCTCTTTGCCAGCCATGTGTACAG
TAAAAAGCAGGCAACATAGTGTCAGCCAAAGCTCATTTGCATAATAAGATTAGGGTGGGGTGGCCAGCTCACATAGGGGTAGGCCCTAGGTAAATCAGAC
ACCGCCTTCTCAAGCCTGTCTATAAAATCTGGTACACTATGACGAGGGTCAGATTTCCCATTCAGACGCCCCTCTCCCATGCAAGAGAAAGAGCTGTTCT
CCTTTCTCTTTCTTTTGCCTATTAAACCTCTGCTCCTGGCCAGGCACAGTGGCTCACGCCTATAATCCCAGCACTTTGGGAGGCTGAGGTGGTCAGATCA
CCTAAGGTCAGGAGTTCAAGACCAGCCTGGTCAACATGGTGAAATCTTGTCTCTAGTAAAAATACAAAAATATATGAAATCTCACATAGATGATAATATT
AAGTTCCAAAAGCAACTCAACCTGGTAGATTCTAATTTTTTTTGAGGCAGGGTCTTGCTTTGTCACCCATGCTGGAGTACAATGGCACAAACACTGCTCA
CTGCAGCCTCGACCTCCCAAGGCCTAAGCAATCCTCCTGCCTCAGTCCCCCTCCAGGTATTTGAAACTACAGGTGTGTACCACCACACCGGGCTAATTTT
TGTATTTTTTGTAGAGACGTGGGTCTCACTATGCTGCCCAGGCTCAGGTCTTAATCTCCTGAGCTCAGGCAATCCGCAGGCCTCAGCCTCCCTAAGTGCG
GGGATTACAGGCTTGAGCCACTGCACCTAGCCTCTATTTGTTTTACAAAAGAGAAATTGAGATCCTGAATGTTAAGTGACTTGCCTGAGGCCATCCCACT
AACAGGAGCCAGGGTTAGGATTCAAACCCCATCCAACTGGTCCCAGAGCTGGAGCTTCTTGCACTGCCCTACACTACCTACCATCTCCATCCTCTGGGCA
CCTTTTTATAAGAACCAAAACATTACAGAGCATTGCTTTGTCAACTCAGCTGGGAACATTTCCCAGTGCAACTCACATTTTTCACTGCTCTGTGCCTGTC
CGTATAAGCTCAATGAGTATTGATTTAGGGGCTTTGGAGAACTTTGAATGCTACCCCCCAAGTAACCATTGTTGGCAACCTGGTACCTCTACTTTTAGCC
ATTTCTCCTTCTCTATAAATAGTGCAGAAGTAACCCACTTGGTAACAGGCATCCTTGCCAAGCCTCCACCACTAGGTCAGTGTAAGAATTAAAGAAAGAG
GAAAGAAACACAAAAAGTGGCTTGATGGTTAAGACAGGTTTATTTTAGAGAAAACACACCTGAGAGGGGCTGCTGGCTGAATTAGGTTAGAGTCTTTTCT
ACAGACTAAGAGTGTTTAAGGATTTAGGGTGGGAGAGTTTCTTAGAGGCTTGGACTGCTTCTGTGTTTTTTTTGTTGTGCTTATATGGGAGGGAGAGTGG
TGTGTTTGCTTTTATACATTTTTCTGCAGCTGTAGGCATACCCCCCAAGTCTGCTTTTAGCTTCCCTATTTTAGTGCACCTGGAGGGAAAGGAATGTGCT
TATTAAGGCCCACTGTTTTACTGGGGCCCATTGTATGAGGGTGAAGTTTGGCAGTTACCCAAGAGACTTTTCCTCCACCTTCCTCTGTGCCCGAGCTGTT
TTATCTGCATTTTACTGTCTGCTTTTTTTGGCTGCTTATAGTTTTTAAAAAAGTAATTTCCTTAAATCCAGAAGGCTAAAAATGAAGCTGAAACTTAAAG
TGGCGGTGTTTGTCCAAAATAACGGGGCTCCTGCTCTGCCAGTCAGTACCCTCAAGTCACTCCTGATCCTCAACCTCCATGCCTAAGGCTGGTTCAAGAG
ACCACATAATATCTGCCTTTTATTACATACATGATGGGTGCATGGGATTCTGCGTGCCCTTTGCTTGATATAGACTGCTAAGGTGAGATGGGGAATATCA
GAGTCAGCTGCTGCTTGAGGAAGCAGAACACACAGCTGGAGGCTTGGAACATGTGGGTCCCTATGAGTGTAGAGCCCATATCCCCATAGAGTCTACCTAG
AGCAGGGGTCGCCAAATGTTTTCTTAAAGAGCCTGATAGTGTATATGTTAGGCTTTGTGAGCCAGGTATTTACAGCAACTCAATTCTACCACTGTGGTAT
GAAAACAGCTATAGACAATCATAAATGAATGATCATGGCTATGTTTTAATAAAACTTTACAGACACTGAACTTGAACTTCCATTGTGATATGAAAACAGC
TATAGACAATCATAAATGAATGATCATGGCTATGTTTTAATAAAACTTTATGGACACTGAGCTTGAACTTCATATATCAGTCATGTGACACAAAATATCA
TTCTTCTTTTTATTTGTTTTCACCCATTTGAAAAATGTAAAAACTATTCTTAGCTGTACAGAAACAGATGGTGGGTCAGATTTGACTCACTGCCCATAGT
TTCCAGACCATGATGTTCAGGTTCATGGAAGCACTTCACTCTACACTAATTTATTCATCCATTCATCACATATGTGATGAGCACATACAGACACACCTCA
GAGATATTGCAGGCTCCACTCCAGATCACCACAATAATGTGAATATTGCAATAAAGCAAGTCATACACATT
- refseq_EMR2.F3 (forward primer)
- refseq_EMR2.R3 (reverse primer)
- Alternative acceptor
Frame 0
GGSALCGEASLLGTCLASEEGPFSLPGLLCGHC
NASVFFLSFCF*HRVSLYHPSWSAVAPS*LTAAS
NSPARVILPASAS*VVAAGTTDVCHQAQLHRQF
RGGPWASGCPPPLLYKVLASGQPAQLPSLRRRD
SPVPLTLSPAAPAGSSAGTMGGRVFLVFLAFCVW
LTLPGAETQDSRGCARWCPQDSSCVNATACRCN
PGFSSFSEIITTPMETCDDINECATLSKVSCGK
FSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESEN
TCQDVDECQQNPRLCKSYGTCVNTLGSYTCQCL
PGFKLKPEDPKLCTDVNECTSGQNPCHSSTHCL
NNVGSYQCRCRPGWQPIPGSPNGPNNTVCEDVDE
CSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRH
GIPNNQKDTVCEDMTFSTWTPPPGVHSQTLSRF
FDKVQDLGRDYKPGLANNTIQSILQALDELLEAP
GDLETLPRLQQHCVASHLLDGLEDVLRGLSKNL
SNGLLNFSYPAGTELSLEVQKQVDRSVTLRQNQ
AVMQLDWNQAQKSGDPGPSVVGLVSIPGMGKLLA
EAPLVLEPEKQMLLHETHQGLLQDGSPILLSDV
ISAFLSNNDTQNLSSPVTFTFSHRSVIPRQKVL
CVFWEHGQNGCGHWATTGCSTIGTRDTSTICRCT
HLSSFAVLMAHYDVQEEDPVLTVITYMGLSVSL
LCLLLAALTFLLCKAIQNTSTSLHLQLSLCLFL
AHLLFLVAIDQTGHKVLCSIIAGTLHYLYLATLT
WMLLEALYLFLTARNLTVVNYSSINRFMKKLMF
PVGYGVPAVTVAISAASRPHLYGTPSRCWLQPE
KGFIWGFLGPVCAIFSVNLVLFLVTLWILKNRLS
SLNSEVSTLRNTRMLAFKATAQLFILGCTWCLG
ILQVGPAARVMAYLFTIINSLQGVFIFLVYCLL
SQQVREQYGKWSKGIRKLKTESEMHTLSSSAKAD
TSKPSTVN*KNLLNKIFPLCPWKI*TIFEPSRG
ERKDFVLCVSRNSPCQQYEGCYGRRARHSIPAE
TGNLPCPAMCSSNSQHMDGQLWPISWSL*STIFM
KL*NVKTSFTASPSYKDSSKS*NEAGKRA*EDF
HTDNI*KD*NVHTTIWIS*FFVFVFVVL*FYGF
DYLVM*KILITHT*IKRDTAPAFMELLGENEVAL
AARVDSEAEIPRNQVSTARQLKYKLFYKHCLLS
SSHQHAEKFLISDRGCATNSISKE*PAKLLNIP
SKCP*PCLLSAFLEQKFYMTIKPRRWVWHSSCPV
ILALWEGEAGGWLKPRSLRPVWATE*EPSLPKK
KIKN*PSMVMI*ELRRNHLGK*QG*EVLSKVFH
LMKSSPQNHFLF*QRTACKIELQT*TSKLEASTG
ECRQLCQ*EKAT*TRHVQNGGSKFPSLCQPCVQ
*KAGNIVSAKAHLHNKIRVGWPAHIGVGPR*IR
HRLLKPVYKIWYTMTRVRFPIQTPLSHARERAVL
LSLSFAY*TSAPGQAQWLTPIIPALWEAEVVRS
PKVRSSRPAWSTW*NLVSSKNTKIYEISHR**Y
*VPKATQPGRF*FFLRQGLALSPMLEYNGTNTAH
CSLDLPRPKQSSCLSPPPGI*NYRCVPPHRANF
CIFCRDVGLTMLPRLRS*SPELRQSAGLSLPKC
GDYRLEPLHLASICFTKEKLRS*MLSDLPEAIPL
TGARVRIQTPSNWSQSWSFLHCPTLPTISILWA
PFYKNQNITEHCFVNSAGNISQCNSHFSLLCAC
PYKLNEY*FRGFGEL*MLPPK*PLLATWYLYF*P
FLLLYK*CRSNPLGNRHPCQASTTRSV*ELKKE
ERNTKSGLMVKTGLF*RKHT*EGLLAELG*SLF
YRLRVFKDLGWESFLEAWTASVFFLLCLYGRESG
VFAFIHFSAAVGIPPKSAFSFPILVHLEGKECA
Y*GPLFYWGPLYEGEVWQLPKRLFLHLPLCPSC
FICILLSAFFGCL*FLKK*FP*IQKAKNEAET*S
GGVCPK*RGSCSASQYPQVTPDPQPPCLRLVQE
TT*YLPFITYMMGAWDSACPLLDIDC*GEMGNI
RVSCCLRKQNTQLEAWNMWVPMSVEPISP*SLPR
AGVAKCFLKEPDSVYVRLCEPGIYSNSILPLWY
ENSYRQS*MNDHGYVLIKLYRH*T*TSIVI*KQ
L*TIINE*SWLCFNKTLWTLSLNFIYQSCDTKYH
SSFYLFSPI*KM*KLFLAVQKQMVGQI*LTAHS
FQTMMFRFMEALHSTLIYSSIHHICDEHIQTHL
RDIAGSTPDHHNNVNIAIKQVIHI
Frame 1
GGLLCAVKLLFLAPAWHRKRAPSPSLGFYVDTV
MPQFSFFLFVFDTGSHSITQAGVQWHHLSSPQP
QTPQLG*FFLPQPPE**QLGPQTSATKPSCTASS
GEGPGPAAVRPPSFIKSWPRDSPHSCPACGDGT
ALSHSLFPLLLLPAAQLEPWEAASFSSFSHSVS
G*LCRELKPRTPGAVPGGALRTPRVSMPPPVAAI
QGSALFLRSSPPPWRLVTTSTSVQHCRKCHAEN
SRTAGTQRGATTACAAQDMSLFLGQKHSRMRAR
TRVKMWTNVSRTQGSVKATAPASTPSAATRASAC
LASSSNLRTRSSAQM*MNAPPDKTHATAPPTAS
TTWAAISAAAARAGNRFRGPPMAQTIPSVKMWT
SAAPGSISVTAPPSASTPWVHTAAAAAQAGSPDT
ESRITKRTLSVKI*LSPPGPRPLESTARRFPDS
STKSRTWAETTSQAWPITPSRASYRRWMSCWRP
LGTWRPCPAYSSTVWPVTCWMA*RMSSEA*ARTF
PMGC*TSVILQAQNCPWRCRSK*TGVSP*DRIR
Q*CSSTGIRHRNLVTQALLWWALSPFQGWASCW
LRPLWSWNLRSRCFCMRHTRACCRTAPPSCSQM*
SLPF*ATTTPKTSAPQLPSPSPTVQ*SRDRRCS
VSSGSMARMDVVTGPPQAAAQ*APETPAPSAVA
PT*AALPSSWPTTMCRRRIPC*LSSPTWG*ASLC
CASSWRPSLFSCVKPSRTPAPHCICSSRSASSW
PTSSSSWQLIKPDTRCCAPSSPVPCTISTWPP*
PGCCWRPCTSSSLHGT*RWSTTQASTDS*RSSCS
LWATESQL*QWPFLQPPGLTFMEHLPAAGSNQK
RDLYGASLDLSAPSSL*I*FSFW*LSGF*KTDS
PPSIVKCPPSGTQGCWHLKRQLSCSSWAARGVWA
SCRWVRLPGSWPTSSPSSTACRVSSSSWCTASS
ASRSGSNMGNGPKGSGN*KLSLRCTHSPAVLRL
TPPNPARLTRKIF*IRSSLFARGKSEQSLSHLEG
KEKTLFCVFQEIHHVSNMKDVMEGVLGIQFLQK
PEIFHALQCAHQTLSIWTASCGPYLGHSEAQYL
*SYRTLRPLSQPLLPTKTPPNLKMKQENEPKRTF
IPTTSERTRMFTPRSGFLNFLFLFLLFSSSTGL
II*SCEKY*LLTHRSRETRLLPSWSF*GKMKWL
LQLELTQKPKFLEIRFLLLGN*SINYFINTVFFR
LHTNMQKSF*SQIGDVQQIPFQRNDLQNS*IFQ
ANALNPVCYLLSLNRNST*P*NLEDGYGTVHAL
*S*HFGRVRQEDGSSPGV*DQCGQQSENHLYPKK
KLKISQAW**YRS*GEII*ANSKGRKSSVRFSI
**KAAPKIIFFSNKEQPVKSSCRHRQASWKLPR
VNAGSCANRKKLPRLGMSKMAAPSSLLFASHVYS
KKQAT*CQPKLICIIRLGWGGQLT*G*ALGKSD
TAFSSLSIKSGTL*RGSDFPFRRPSPMQEKELF
SFLFLLPIKPLLLARHSGSRL*SQHFGRLRWSDH
LRSGVQDQPGQHGEILSLVKIQKYMKSHIDDNI
KFQKQLNLVDSNFF*GRVLLCHPCWSTMAQTLL
TAASTSQGLSNPPASVPLQVFETTGVYHHTGLIF
VFFVETWVSLCCPGSGLNLLSSGNPQASASLSA
GITGLSHCT*PLFVLQKRN*DPEC*VTCLRPSH
*QEPGLGFKPHPTGPRAGASCTALHYLPSPSSGH
LFIRTKTLQSIALSTQLGTFPSATHIFHCSVPV
RISSMSIDLGALENFECYPPSNHCWQPGTSTFS
HFSFSINSAEVTHLVTGILAKPPPLGQCKN*RKR
KETQKVA*WLRQVYFRENTPERGCWLN*VRVFS
TD*ECLRI*GGRVS*RLGLLLCFFCCAYMGGRV
VCLLLYIFLQL*AYPPSLLLASLF*CTWRERNVL
IKAHCFTGAHCMRVKFGSYPRDFSSTFLCARAV
LSAFYCLLFLAAYSF*KSNFLKSRRLKMKLKLK
VAVFVQNNGAPALPVSTLKSLLILNLHA*GWFKR
PHNICLLLHT*WVHGILRALCLI*TAKVRWGIS
ESAAA*GSRTHSWRLGTCGSL*V*SPYPHRVYL
EQGSPNVFLKSLIVYMLGFVSQVFTATQFYHCGM
KTAIDNHK*MIMAMF**NFTDTELELPL*YENS
YRQS*MNDHGYVLIKLYGH*A*TSYISHVTQNI
ILLFICFHPFEKCKNYS*LYRNRWWVRFDSLPIV
SRP*CSGSWKHFTLH*FIHPFITYVMSTYRHTS
EILQAPLQITTIM*ILQ*SKSYT
Frame 2
GVCSVR*SFSSWHLPGIGRGPLLPPWAFMWTL*
CLSFLSFFLFLTQGLTLSPKLECSGTILAHRSL
KLPSSGDSSCLSLLSSSSWDHRRLPPSPAAPPV
PGRALGQRLSAPPPL*SPGLGTARTAAQPAETGQ
PCPTHSFPCCSCRQLSWNHGRPRLSRLSRILCL
ADSAGS*NPGLQGLCPVVPSGLLVCQCHRLSLQ
SRVQLFF*DHHHPHGDL*RHQRVCNTVESVMRKI
LGLLEHRGELRLRVQPRI*ACFWGKNIQE*ERE
HVSRCGRMSAEPKAL*KLRHLRQHPRQLHVPVP
AWLQAQT*GPEALHRCE*MHLRTKPMPQLHPLPQ
QRGQLSVPLPPGLATDSGVPQWPKQYRL*RCGR
VQLRAASV*QLHRLLQHRGFIQLPLPPRLEAQT
RNPE*PKGHCL*RYDFLHLDPAPWSPQPDAFPIL
RQSPGPGQRLQARLGQ*HHPEHLTGAG*AAGGP
WGPGDPAPLTAALCGQSPAGWPRGCPQRPEQEP
FQWAVELQLSCRHRIVPGGAEASRQECHLETESG
SDAARLESGTEIW*PRPFCGGPCLHSRDGQVAG
*GPSGPGT*EADASA*DTPGLAAGRLPHPALRC
DLCLSEQQRHPKPQLPSYLHLLPPFSDPETEGAL
CLLGAWPEWMWSLGHHRLQHNRHQRHQHHLPLH
PPEQLCRPHGPLRCAGGGSRADCHHLHGAERLS
AVPPPGGPHFSPV*SHPEHQHLTASAALALPLPG
PPPLPRGN*SNRTQGAVLHHRRYLALSLPGHLD
LDAAGGPVPLPHCTEPDGGQLLKHQQIHEEAHV
PCGLRSPSCDSGHFCSLQASPLWNTFPLLAPTRK
GIYMGLPWTCLRHLLCEFSSLSGDSLDFEKQTL
LPQ**SVHPPEHKDAGI*SDSSAVHPGLHVVSG
HLAGGSGCPGHGLPLHHHQQPAGCLHLPGVLPPQ
PAGPGAIWEMVQRDQEIEN*V*DAHTLQQC*G*
HLQTQHG*LEKSSE*DLPSLPVENLNNL*AI*R
GKKRLCSVCFKKFTMSAI*RMLWKAC*AFNSCRN
RKSSMPCNVLIKLSAYGRPAVAHILVTLKHNIY
EAIER*DLFHSLSFLQRLLQILK*SRKTSLRGL
SYRQHLKGLECSHHDLDFLIFCFCFCCSLVLRV*
LFSHVKNIDYSHIDQERHGSCLHGAFRGK*SGS
CS*S*LRSRNS*KSGFYC*AIEV*TIL*TLSSF
VFTPTCRKVSNLRSGMCNKFHFKGMTCKTPKYSK
QMPLTLSVICFP*TEILHDHKTSKMGMAQFMPC
NPSTLGG*GRRMAQAQEFETSVGNRVRTISTQK
KN*KLAKHGDDIGVKEKSFRQIARVGSPQ*GFPF
NEKQPPKSFSFLTKNSL*NRAADIDKQAGSFHG
*MPAAVPIGKSYLD*ACPKWRLQVPFSLPAMCT
VKSRQHSVSQSSFA**D*GGVASSHRGRP*VNQT
PPSQACL*NLVHYDEGQISHSDAPLPCKRKSCS
PFSFFCLLNLCSWPGTVAHAYNPSTLGG*GGQI
T*GQEFKTSLVNMVKSCL**KYKNI*NLT*MIIL
SSKSNSTW*ILIFFEAGSCFVTHAGVQWHKHCS
LQPRPPKA*AILLPQSPSRYLKLQVCTTTPG*F
LYFL*RRGSHYAAQAQVLIS*AQAIRRPQPP*VR
GLQA*ATAPSLYLFYKREIEILNVK*LA*GHPT
NRSQG*DSNPIQLVPELELLALPYTTYHLHPLG
TFL*EPKHYRALLCQLSWEHFPVQLTFFTALCLS
V*AQ*VLI*GLWRTLNATPQVTIVGNLVPLLLA
ISPSL*IVQK*PTW*QASLPSLHH*VSVRIKER
GKKHKKWLDG*DRFILEKTHLRGAAG*IRLESFL
QTKSV*GFRVGEFLRGLDCFCVFFVVLIWEGEW
CVCFYTFFCSCRHTPQVCF*LPYFSAPGGKGMC
LLRPTVLLGPIV*G*SLAVTQETFPPPSSVPELF
YLHFTVCFFWLLIVFKKVISLNPEG*K*S*NLK
WRCLSKITGLLLCQSVPSSHS*SSTSMPKAGSR
DHIISAFYYIHDGCMGFCVPFA*YRLLR*DGEYQ
SQLLLEEAEHTAGGLEHVGPYECRAHIPIEST*
SRGRQMFS*RA**CIC*AL*ARYLQQLNSTTVV
*KQL*TIINE*SWLCFNKTLQTLNLNFHCDMKTA
IDNHK*MIMAMF**NFMDTELELHISVM*HKIS
FFFLFVFTHLKNVKTILSCTETDGGSDLTHCP*
FPDHDVQVHGSTSLYTNLFIHSSHM**AHTDTPQ
RYCRLHSRSPQ*CEYCNKASHTH
Sequence NM_152917
GGGGGGTCTGCTCTGTGCGGTGAAGCTTCTCTTCTTGGCACCTGCCTGGCATCGGAAGAGGGCCCCTTCTCCCTCCCTGGGCTTTTATGTGGACACTGTA
ATGCCTCAGTTTTCTTTCTTTCTTTTTGTTTTTGACACAGGGTCTCACTCTATCACCCAAGCTGGAGTGCAGTGGCACCATCTTAGCTCACCGCAGCCTC
AAACTCCCCAGCTCGGGTGATTCTTCCTGCCTCAGCCTCCTGAGTAGTAGCAGCTGGGACCACAGACGTCTGCCACCAAGCCCAGCTGCACCGCCAGTTC
CGGGGAGGGCCCTGGGCCAGCGGCTGTCCGCCCCCCCTCCTTTATAAAGTCCTGGCCTCGGGACAGCCCGCACAGCTGCCCAGCCTGCGGAGACGGGACA
GCCCTGTCCCACTCACTCTTTCCCCTGCTGCTCCTGCCGGCAGCTCAGCTGGAACCATGGGAGGCCGCGTCTTTCTCGTCTTTCTCGCATTCTGTGTCTG
GCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGGACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAAT
CCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCCATGGAGACTTGTGACGACATCAACGAGTGTGCAACACTGTCGAAAGTGTCATGCGGAAAAT
TCTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCAGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAA
CACGTGTCAAGATGTGGACGAGTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCAACACCGTGGGTTCATACAGCTGCCGCTGCCGC
CCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACACTGTCTGTGAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACA
GCCAGACGCTTTCCCGATTCTTCGACAAAGTCCAGGACCTGGGCAGAGACTACAAGCCAGGCTTGGCCAATAACACCATCCAGAGCATCTTACAGGCGCT
GGATGAGCTGCTGGAGGCCCCTGGGGACCTGGAGACCCTGCCCCGCTTACAGCAGCACTGTGTGGCCAGTCACCTGCTGGATGGCCTAGAGGATGTCCTC
AGAGGCCTGAGCAAGAACCTTTCCAATGGGCTGTTGAACTTCAGTTATCCTGCAGGCACAGAATTGTCCCTGGAGGTGCAGAAGCAAGTAGACAGGAGTG
TCACCTTGAGACAGAATCAGGCAGTGATGCAGCTCGACTGGAATCAGGCACAGAAATCTGGTGACCCAGGCCCTTCTGTGGTGGGCCTTGTCTCCATTCC
AGGGATGGGCAAGTTGCTGGCTGAGGCCCCTCTGGTCCTGGAACCTGAGAAGCAGATGCTTCTGCATGAGACACACCAGGGCTTGCTGCAGGACGGCTCC
CCCATCCTGCTCTCAGATGTGATCTCTGCCTTTCTGAGCAACAACGACACCCAAAACCTCAGCTCCCCAGTTACCTTCACCTTCTCCCACCGTTCAGTGA
TCCCGAGACAGAAGGTGCTCTGTGTCTTCTGGGAGCATGGCCAGAATGGATGTGGTCACTGGGCCACCACAGGCTGCAGCACAATAGGCACCAGAGACAC
CAGCACCATCTGCCGTTGCACCCACCTGAGCAGCTTTGCCGTCCTCATGGCCCACTACGATGTGCAGGAGGAGGATCCCGTGCTGACTGTCATCACCTAC
ATGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTCCTGGCGGCCCTCACTTTTCTCCTGTGTAAAGCCATCCAGAACACCAGCACCTCACTGCATCTGCAGC
TCTCGCTCTGCCTCTTCCTGGCCCACCTCCTCTTCCTCGTGGCAATTGATCAAACCGGACACAAGGTGCTGTGCTCCATCATCGCCGGTACCTTGCACTA
TCTCTACCTGGCCACCTTGACCTGGATGCTGCTGGAGGCCCTGTACCTCTTCCTCACTGCACGGAACCTGACGGTGGTCAACTACTCAAGCATCAACAGA
TTCATGAAGAAGCTCATGTTCCCTGTGGGCTACGGAGTCCCAGCTGTGACAGTGGCCATTTCTGCAGCCTCCAGGCCTCACCTTTATGGAACACCTTCCC
GCTGCTGGCTCCAACCAGAAAAGGGATTTATATGGGGCTTCCTTGGACCTGTCTGCGCCATCTTCTCTGTGAATTTAGTTCTCTTTCTGGTGACTCTCTG
GATTTTGAAAAACAGACTCTCCTCCCTCAATAGTGAAGTGTCCACCCTCCGGAACACAAGGATGCTGGCATTTAAAGCGACAGCTCAGCTGTTCATCCTG
GGCTGCACGTGGTGTCTGGGCATCTTGCAGGTGGGTCCGGCTGCCCGGGTCATGGCCTACCTCTTCACCATCATCAACAGCCTGCAGGGTGTCTTCATCT
TCCTGGTGTACTGCCTCCTCAGCCAGCAGGTCCGGGAGCAATATGGGAAATGGTCCAAAGGGATCAGGAAATTGAAAACTGAGTCTGAGATGCACACACT
CTCCAGCAGTGCTAAGGCTGACACCTCCAAACCCAGCACGGTTAACTAGAAAAATCTTCTGAATAAGATCTTCCCTCTTTGCCCGTGGAAAATCTGAACA
ATCTTTGAGCCATCTAGAGGGGAAAGAAAAGACTTTGTTCTGTGTGTTTCAAGAAATTCACCATGTCAGCAATATGAAGGATGTTATGGAAGGCGTGCTA
GGCATTCAATTCCTGCAGAAACCGGAAATCTTCCATGCCCTGCAATGTGCTCATCAAACTCTCAGCATATGGACGGCCAGCTGTGGCCCATATCTTGGTC
ACTCTGAAGCACAATATTTATGAAGCTATAGAACGTTAAGACCTCTTTCACAGCCTCTCCTTCCTACAAAGACTCCTCCAAATCTTAAAATGAAGCAGGA
AAACGAGCCTAAGAGGACTTTCATACCGACAACATCTGAAAGGACTAGAATGTTCACACCACGATCTGGATTTCTTAATTTTTTGTTTTTGTTTTTGTTG
TTCTCTAGTTCTACGGGTTTGATTATTTAGTCATGTGAAAAATATTGATTACTCACACATAGATCAAGAGAGACACGGCTCCTGCCTTCATGGAGCTTTT
AGGGGAAAATGAAGTGGCTCTTGCAGCTAGAGTTGACTCAGAAGCCGAAATTCCTAGAAATCAGGTTTCTACTGCTAGGCAATTGAAGTATAAACTATTT
TATAAACACTGTCTTCTTTCGTCTTCACACCAACATGCAGAAAAGTTTCTAATCTCAGATCGGGGATGTGCAACAAATTCCATTTCAAAGGAATGACCTG
CAAAACTCCTAAATATTCCAAGCAAATGCCCTTAACCCTGTCTGTTATCTGCTTTCCTTGAACAGAAATTCTACATGACCATAAAACCTCGAAGATGGGT
ATGGCACAGTTCATGCCCTGTAATCCTAGCACTTTGGGAGGGTGAGGCAGGAGGATGGCTCAAGCCCAGGAGTTTGAGACCAGTGTGGGCAACAGAGTGA
GAACCATCTCTACCCAAAAAAAAAATTAAAAATTAGCCAAGCATGGTGATGATATAGGAGTTAAGGAGAAATCATTTAGGCAAATAGCAAGGGTAGGAAG
TCCTCAGTAAGGTTTTCCATTTAATGAAAAGCAGCCCCCAAAATCATTTTCTTTTCTAACAAAGAACAGCCTGTAAAATCGAGCTGCAGACATAGACAAG
CAAGCTGGAAGCTTCCACGGGTGAATGCCGGCAGCTGTGCCAATAGGAAAAAGCTACCTAGACTAGGCATGTCCAAAATGGCGGCTCCAAGTTCCCTTCT
CTTTGCCAGCCATGTGTACAGTAAAAAGCAGGCAACATAGTGTCAGCCAAAGCTCATTTGCATAATAAGATTAGGGTGGGGTGGCCAGCTCACATAGGGG
TAGGCCCTAGGTAAATCAGACACCGCCTTCTCAAGCCTGTCTATAAAATCTGGTACACTATGACGAGGGTCAGATTTCCCATTCAGACGCCCCTCTCCCA
TGCAAGAGAAAGAGCTGTTCTCCTTTCTCTTTCTTTTGCCTATTAAACCTCTGCTCCTGGCCAGGCACAGTGGCTCACGCCTATAATCCCAGCACTTTGG
GAGGCTGAGGTGGTCAGATCACCTAAGGTCAGGAGTTCAAGACCAGCCTGGTCAACATGGTGAAATCTTGTCTCTAGTAAAAATACAAAAATATATGAAA
TCTCACATAGATGATAATATTAAGTTCCAAAAGCAACTCAACCTGGTAGATTCTAATTTTTTTTGAGGCAGGGTCTTGCTTTGTCACCCATGCTGGAGTA
CAATGGCACAAACACTGCTCACTGCAGCCTCGACCTCCCAAGGCCTAAGCAATCCTCCTGCCTCAGTCCCCCTCCAGGTATTTGAAACTACAGGTGTGTA
CCACCACACCGGGCTAATTTTTGTATTTTTTGTAGAGACGTGGGTCTCACTATGCTGCCCAGGCTCAGGTCTTAATCTCCTGAGCTCAGGCAATCCGCAG
GCCTCAGCCTCCCTAAGTGCGGGGATTACAGGCTTGAGCCACTGCACCTAGCCTCTATTTGTTTTACAAAAGAGAAATTGAGATCCTGAATGTTAAGTGA
CTTGCCTGAGGCCATCCCACTAACAGGAGCCAGGGTTAGGATTCAAACCCCATCCAACTGGTCCCAGAGCTGGAGCTTCTTGCACTGCCCTACACTACCT
ACCATCTCCATCCTCTGGGCACCTTTTTATAAGAACCAAAACATTACAGAGCATTGCTTTGTCAACTCAGCTGGGAACATTTCCCAGTGCAACTCACATT
TTTCACTGCTCTGTGCCTGTCCGTATAAGCTCAATGAGTATTGATTTAGGGGCTTTGGAGAACTTTGAATGCTACCCCCCAAGTAACCATTGTTGGCAAC
CTGGTACCTCTACTTTTAGCCATTTCTCCTTCTCTATAAATAGTGCAGAAGTAACCCACTTGGTAACAGGCATCCTTGCCAAGCCTCCACCACTAGGTCA
GTGTAAGAATTAAAGAAAGAGGAAAGAAACACAAAAAGTGGCTTGATGGTTAAGACAGGTTTATTTTAGAGAAAACACACCTGAGAGGGGCTGCTGGCTG
AATTAGGTTAGAGTCTTTTCTACAGACTAAGAGTGTTTAAGGATTTAGGGTGGGAGAGTTTCTTAGAGGCTTGGACTGCTTCTGTGTTTTTTTTGTTGTG
CTTATATGGGAGGGAGAGTGGTGTGTTTGCTTTTATACATTTTTCTGCAGCTGTAGGCATACCCCCCAAGTCTGCTTTTAGCTTCCCTATTTTAGTGCAC
CTGGAGGGAAAGGAATGTGCTTATTAAGGCCCACTGTTTTACTGGGGCCCATTGTATGAGGGTGAAGTTTGGCAGTTACCCAAGAGACTTTTCCTCCACC
TTCCTCTGTGCCCGAGCTGTTTTATCTGCATTTTACTGTCTGCTTTTTTTGGCTGCTTATAGTTTTTAAAAAAGTAATTTCCTTAAATCCAGAAGGCTAA
AAATGAAGCTGAAACTTAAAGTGGCGGTGTTTGTCCAAAATAACGGGGCTCCTGCTCTGCCAGTCAGTACCCTCAAGTCACTCCTGATCCTCAACCTCCA
TGCCTAAGGCTGGTTCAAGAGACCACATAATATCTGCCTTTTATTACATACATGATGGGTGCATGGGATTCTGCGTGCCCTTTGCTTGATATAGACTGCT
AAGGTGAGATGGGGAATATCAGAGTCAGCTGCTGCTTGAGGAAGCAGAACACACAGCTGGAGGCTTGGAACATGTGGGTCCCTATGAGTGTAGAGCCCAT
ATCCCCATAGAGTCTACCTAGAGCAGGGGTCGCCAAATGTTTTCTTAAAGAGCCTGATAGTGTATATGTTAGGCTTTGTGAGCCAGGTATTTACAGCAAC
TCAATTCTACCACTGTGGTATGAAAACAGCTATAGACAATCATAAATGAATGATCATGGCTATGTTTTAATAAAACTTTACAGACACTGAACTTGAACTT
CCATTGTGATATGAAAACAGCTATAGACAATCATAAATGAATGATCATGGCTATGTTTTAATAAAACTTTATGGACACTGAGCTTGAACTTCATATATCA
GTCATGTGACACAAAATATCATTCTTCTTTTTATTTGTTTTCACCCATTTGAAAAATGTAAAAACTATTCTTAGCTGTACAGAAACAGATGGTGGGTCAG
ATTTGACTCACTGCCCATAGTTTCCAGACCATGATGTTCAGGTTCATGGAAGCACTTCACTCTACACTAATTTATTCATCCATTCATCACATATGTGATG
AGCACATACAGACACACCTCAGAGATATTGCAGGCTCCACTCCAGATCACCACAATAATGTGAATATTGCAATAAAGCAAGTCATACACATT
- refseq_EMR2.F3 (forward primer)
- refseq_EMR2.R3 (reverse primer)
- Alternative acceptor
Frame 0
GGSALCGEASLLGTCLASEEGPFSLPGLLCGHC
NASVFFLSFCF*HRVSLYHPSWSAVAPS*LTAAS
NSPARVILPASAS*VVAAGTTDVCHQAQLHRQF
RGGPWASGCPPPLLYKVLASGQPAQLPSLRRRD
SPVPLTLSPAAPAGSSAGTMGGRVFLVFLAFCVW
LTLPGAETQDSRGCARWCPQDSSCVNATACRCN
PGFSSFSEIITTPMETCDDINECATLSKVSCGK
FSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESEN
TCQDVDECSSGQHQCDSSTVCFNTVGSYSCRCR
PGWKPRHGIPNNQKDTVCEDMTFSTWTPPPGVH
SQTLSRFFDKVQDLGRDYKPGLANNTIQSILQAL
DELLEAPGDLETLPRLQQHCVASHLLDGLEDVL
RGLSKNLSNGLLNFSYPAGTELSLEVQKQVDRS
VTLRQNQAVMQLDWNQAQKSGDPGPSVVGLVSIP
GMGKLLAEAPLVLEPEKQMLLHETHQGLLQDGS
PILLSDVISAFLSNNDTQNLSSPVTFTFSHRSV
IPRQKVLCVFWEHGQNGCGHWATTGCSTIGTRDT
STICRCTHLSSFAVLMAHYDVQEEDPVLTVITY
MGLSVSLLCLLLAALTFLLCKAIQNTSTSLHLQ
LSLCLFLAHLLFLVAIDQTGHKVLCSIIAGTLHY
LYLATLTWMLLEALYLFLTARNLTVVNYSSINR
FMKKLMFPVGYGVPAVTVAISAASRPHLYGTPS
RCWLQPEKGFIWGFLGPVCAIFSVNLVLFLVTLW
ILKNRLSSLNSEVSTLRNTRMLAFKATAQLFIL
GCTWCLGILQVGPAARVMAYLFTIINSLQGVFI
FLVYCLLSQQVREQYGKWSKGIRKLKTESEMHTL
SSSAKADTSKPSTVN*KNLLNKIFPLCPWKI*T
IFEPSRGERKDFVLCVSRNSPCQQYEGCYGRRA
RHSIPAETGNLPCPAMCSSNSQHMDGQLWPISWS
L*STIFMKL*NVKTSFTASPSYKDSSKS*NEAG
KRA*EDFHTDNI*KD*NVHTTIWIS*FFVFVFV
VL*FYGFDYLVM*KILITHT*IKRDTAPAFMELL
GENEVALAARVDSEAEIPRNQVSTARQLKYKLF
YKHCLLSSSHQHAEKFLISDRGCATNSISKE*P
AKLLNIPSKCP*PCLLSAFLEQKFYMTIKPRRWV
WHSSCPVILALWEGEAGGWLKPRSLRPVWATE*
EPSLPKKKIKN*PSMVMI*ELRRNHLGK*QG*E
VLSKVFHLMKSSPQNHFLF*QRTACKIELQT*TS
KLEASTGECRQLCQ*EKAT*TRHVQNGGSKFPS
LCQPCVQ*KAGNIVSAKAHLHNKIRVGWPAHIG
VGPR*IRHRLLKPVYKIWYTMTRVRFPIQTPLSH
ARERAVLLSLSFAY*TSAPGQAQWLTPIIPALW
EAEVVRSPKVRSSRPAWSTW*NLVSSKNTKIYE
ISHR**Y*VPKATQPGRF*FFLRQGLALSPMLEY
NGTNTAHCSLDLPRPKQSSCLSPPPGI*NYRCV
PPHRANFCIFCRDVGLTMLPRLRS*SPELRQSA
GLSLPKCGDYRLEPLHLASICFTKEKLRS*MLSD
LPEAIPLTGARVRIQTPSNWSQSWSFLHCPTLP
TISILWAPFYKNQNITEHCFVNSAGNISQCNSH
FSLLCACPYKLNEY*FRGFGEL*MLPPK*PLLAT
WYLYF*PFLLLYK*CRSNPLGNRHPCQASTTRS
V*ELKKEERNTKSGLMVKTGLF*RKHT*EGLLA
ELG*SLFYRLRVFKDLGWESFLEAWTASVFFLLC
LYGRESGVFAFIHFSAAVGIPPKSAFSFPILVH
LEGKECAY*GPLFYWGPLYEGEVWQLPKRLFLH
LPLCPSCFICILLSAFFGCL*FLKK*FP*IQKAK
NEAET*SGGVCPK*RGSCSASQYPQVTPDPQPP
CLRLVQETT*YLPFITYMMGAWDSACPLLDIDC
*GEMGNIRVSCCLRKQNTQLEAWNMWVPMSVEPI
SP*SLPRAGVAKCFLKEPDSVYVRLCEPGIYSN
SILPLWYENSYRQS*MNDHGYVLIKLYRH*T*T
SIVI*KQL*TIINE*SWLCFNKTLWTLSLNFIYQ
SCDTKYHSSFYLFSPI*KM*KLFLAVQKQMVGQ
I*LTAHSFQTMMFRFMEALHSTLIYSSIHHICD
EHIQTHLRDIAGSTPDHHNNVNIAIKQVIHI
Frame 1
GGLLCAVKLLFLAPAWHRKRAPSPSLGFYVDTV
MPQFSFFLFVFDTGSHSITQAGVQWHHLSSPQP
QTPQLG*FFLPQPPE**QLGPQTSATKPSCTASS
GEGPGPAAVRPPSFIKSWPRDSPHSCPACGDGT
ALSHSLFPLLLLPAAQLEPWEAASFSSFSHSVS
G*LCRELKPRTPGAVPGGALRTPRVSMPPPVAAI
QGSALFLRSSPPPWRLVTTSTSVQHCRKCHAEN
SRTAGTQRGATTACAAQDMSLFLGQKHSRMRAR
TRVKMWTSAAPGSISVTAPPSASTPWVHTAAAAA
QAGSPDTESRITKRTLSVKI*LSPPGPRPLEST
ARRFPDSSTKSRTWAETTSQAWPITPSRASYRR
WMSCWRPLGTWRPCPAYSSTVWPVTCWMA*RMSS
EA*ARTFPMGC*TSVILQAQNCPWRCRSK*TGV
SP*DRIRQ*CSSTGIRHRNLVTQALLWWALSPF
QGWASCWLRPLWSWNLRSRCFCMRHTRACCRTAP
PSCSQM*SLPF*ATTTPKTSAPQLPSPSPTVQ*
SRDRRCSVSSGSMARMDVVTGPPQAAAQ*APET
PAPSAVAPT*AALPSSWPTTMCRRRIPC*LSSPT
WG*ASLCCASSWRPSLFSCVKPSRTPAPHCICS
SRSASSWPTSSSSWQLIKPDTRCCAPSSPVPCT
ISTWPP*PGCCWRPCTSSSLHGT*RWSTTQASTD
S*RSSCSLWATESQL*QWPFLQPPGLTFMEHLP
AAGSNQKRDLYGASLDLSAPSSL*I*FSFW*LS
GF*KTDSPPSIVKCPPSGTQGCWHLKRQLSCSSW
AARGVWASCRWVRLPGSWPTSSPSSTACRVSSS
SWCTASSASRSGSNMGNGPKGSGN*KLSLRCTH
SPAVLRLTPPNPARLTRKIF*IRSSLFARGKSEQ
SLSHLEGKEKTLFCVFQEIHHVSNMKDVMEGVL
GIQFLQKPEIFHALQCAHQTLSIWTASCGPYLG
HSEAQYL*SYRTLRPLSQPLLPTKTPPNLKMKQE
NEPKRTFIPTTSERTRMFTPRSGFLNFLFLFLL
FSSSTGLII*SCEKY*LLTHRSRETRLLPSWSF
*GKMKWLLQLELTQKPKFLEIRFLLLGN*SINYF
INTVFFRLHTNMQKSF*SQIGDVQQIPFQRNDL
QNS*IFQANALNPVCYLLSLNRNST*P*NLEDG
YGTVHAL*S*HFGRVRQEDGSSPGV*DQCGQQSE
NHLYPKKKLKISQAW**YRS*GEII*ANSKGRK
SSVRFSI**KAAPKIIFFSNKEQPVKSSCRHRQ
ASWKLPRVNAGSCANRKKLPRLGMSKMAAPSSLL
FASHVYSKKQAT*CQPKLICIIRLGWGGQLT*G
*ALGKSDTAFSSLSIKSGTL*RGSDFPFRRPSP
MQEKELFSFLFLLPIKPLLLARHSGSRL*SQHFG
RLRWSDHLRSGVQDQPGQHGEILSLVKIQKYMK
SHIDDNIKFQKQLNLVDSNFF*GRVLLCHPCWS
TMAQTLLTAASTSQGLSNPPASVPLQVFETTGVY
HHTGLIFVFFVETWVSLCCPGSGLNLLSSGNPQ
ASASLSAGITGLSHCT*PLFVLQKRN*DPEC*V
TCLRPSH*QEPGLGFKPHPTGPRAGASCTALHYL
PSPSSGHLFIRTKTLQSIALSTQLGTFPSATHI
FHCSVPVRISSMSIDLGALENFECYPPSNHCWQ
PGTSTFSHFSFSINSAEVTHLVTGILAKPPPLGQ
CKN*RKRKETQKVA*WLRQVYFRENTPERGCWL
N*VRVFSTD*ECLRI*GGRVS*RLGLLLCFFCC
AYMGGRVVCLLLYIFLQL*AYPPSLLLASLF*CT
WRERNVLIKAHCFTGAHCMRVKFGSYPRDFSST
FLCARAVLSAFYCLLFLAAYSF*KSNFLKSRRL
KMKLKLKVAVFVQNNGAPALPVSTLKSLLILNLH
A*GWFKRPHNICLLLHT*WVHGILRALCLI*TA
KVRWGISESAAA*GSRTHSWRLGTCGSL*V*SP
YPHRVYLEQGSPNVFLKSLIVYMLGFVSQVFTAT
QFYHCGMKTAIDNHK*MIMAMF**NFTDTELEL
PL*YENSYRQS*MNDHGYVLIKLYGH*A*TSYI
SHVTQNIILLFICFHPFEKCKNYS*LYRNRWWVR
FDSLPIVSRP*CSGSWKHFTLH*FIHPFITYVM
STYRHTSEILQAPLQITTIM*ILQ*SKSYT
Frame 2
GVCSVR*SFSSWHLPGIGRGPLLPPWAFMWTL*
CLSFLSFFLFLTQGLTLSPKLECSGTILAHRSL
KLPSSGDSSCLSLLSSSSWDHRRLPPSPAAPPV
PGRALGQRLSAPPPL*SPGLGTARTAAQPAETGQ
PCPTHSFPCCSCRQLSWNHGRPRLSRLSRILCL
ADSAGS*NPGLQGLCPVVPSGLLVCQCHRLSLQ
SRVQLFF*DHHHPHGDL*RHQRVCNTVESVMRKI
LGLLEHRGELRLRVQPRI*ACFWGKNIQE*ERE
HVSRCGRVQLRAASV*QLHRLLQHRGFIQLPLP
PRLEAQTRNPE*PKGHCL*RYDFLHLDPAPWSPQ
PDAFPILRQSPGPGQRLQARLGQ*HHPEHLTGA
G*AAGGPWGPGDPAPLTAALCGQSPAGWPRGCP
QRPEQEPFQWAVELQLSCRHRIVPGGAEASRQEC
HLETESGSDAARLESGTEIW*PRPFCGGPCLHS
RDGQVAG*GPSGPGT*EADASA*DTPGLAAGRL
PHPALRCDLCLSEQQRHPKPQLPSYLHLLPPFSD
PETEGALCLLGAWPEWMWSLGHHRLQHNRHQRH
QHHLPLHPPEQLCRPHGPLRCAGGGSRADCHHL
HGAERLSAVPPPGGPHFSPV*SHPEHQHLTASAA
LALPLPGPPPLPRGN*SNRTQGAVLHHRRYLAL
SLPGHLDLDAAGGPVPLPHCTEPDGGQLLKHQQ
IHEEAHVPCGLRSPSCDSGHFCSLQASPLWNTFP
LLAPTRKGIYMGLPWTCLRHLLCEFSSLSGDSL
DFEKQTLLPQ**SVHPPEHKDAGI*SDSSAVHP
GLHVVSGHLAGGSGCPGHGLPLHHHQQPAGCLHL
PGVLPPQPAGPGAIWEMVQRDQEIEN*V*DAHT
LQQC*G*HLQTQHG*LEKSSE*DLPSLPVENLN
NL*AI*RGKKRLCSVCFKKFTMSAI*RMLWKAC*
AFNSCRNRKSSMPCNVLIKLSAYGRPAVAHILV
TLKHNIYEAIER*DLFHSLSFLQRLLQILK*SR
KTSLRGLSYRQHLKGLECSHHDLDFLIFCFCFCC
SLVLRV*LFSHVKNIDYSHIDQERHGSCLHGAF
RGK*SGSCS*S*LRSRNS*KSGFYC*AIEV*TI
L*TLSSFVFTPTCRKVSNLRSGMCNKFHFKGMTC
KTPKYSKQMPLTLSVICFP*TEILHDHKTSKMG
MAQFMPCNPSTLGG*GRRMAQAQEFETSVGNRV
RTISTQKKN*KLAKHGDDIGVKEKSFRQIARVGS
PQ*GFPFNEKQPPKSFSFLTKNSL*NRAADIDK
QAGSFHG*MPAAVPIGKSYLD*ACPKWRLQVPF
SLPAMCTVKSRQHSVSQSSFA**D*GGVASSHRG
RP*VNQTPPSQACL*NLVHYDEGQISHSDAPLP
CKRKSCSPFSFFCLLNLCSWPGTVAHAYNPSTL
GG*GGQIT*GQEFKTSLVNMVKSCL**KYKNI*N
LT*MIILSSKSNSTW*ILIFFEAGSCFVTHAGV
QWHKHCSLQPRPPKA*AILLPQSPSRYLKLQVC
TTTPG*FLYFL*RRGSHYAAQAQVLIS*AQAIRR
PQPP*VRGLQA*ATAPSLYLFYKREIEILNVK*
LA*GHPTNRSQG*DSNPIQLVPELELLALPYTT
YHLHPLGTFL*EPKHYRALLCQLSWEHFPVQLTF
FTALCLSV*AQ*VLI*GLWRTLNATPQVTIVGN
LVPLLLAISPSL*IVQK*PTW*QASLPSLHH*V
SVRIKERGKKHKKWLDG*DRFILEKTHLRGAAG*
IRLESFLQTKSV*GFRVGEFLRGLDCFCVFFVV
LIWEGEWCVCFYTFFCSCRHTPQVCF*LPYFSA
PGGKGMCLLRPTVLLGPIV*G*SLAVTQETFPPP
SSVPELFYLHFTVCFFWLLIVFKKVISLNPEG*
K*S*NLKWRCLSKITGLLLCQSVPSSHS*SSTS
MPKAGSRDHIISAFYYIHDGCMGFCVPFA*YRLL
R*DGEYQSQLLLEEAEHTAGGLEHVGPYECRAH
IPIEST*SRGRQMFS*RA**CIC*AL*ARYLQQ
LNSTTVV*KQL*TIINE*SWLCFNKTLQTLNLNF
HCDMKTAIDNHK*MIMAMF**NFMDTELELHIS
VM*HKISFFFLFVFTHLKNVKTILSCTETDGGS
DLTHCP*FPDHDVQVHGSTSLYTNLFIHSSHM**
AHTDTPQRYCRLHSRSPQ*CEYCNKASHTH
Sequence NM_152916
GGGGGGTCTGCTCTGTGCGGTGAAGCTTCTCTTCTTGGCACCTGCCTGGCATCGGAAGAGGGCCCCTTCTCCCTCCCTGGGCTTTTATGTGGACACTGTA
ATGCCTCAGTTTTCTTTCTTTCTTTTTGTTTTTGACACAGGGTCTCACTCTATCACCCAAGCTGGAGTGCAGTGGCACCATCTTAGCTCACCGCAGCCTC
AAACTCCCCAGCTCGGGTGATTCTTCCTGCCTCAGCCTCCTGAGTAGTAGCAGCTGGGACCACAGACGTCTGCCACCAAGCCCAGCTGCACCGCCAGTTC
CGGGGAGGGCCCTGGGCCAGCGGCTGTCCGCCCCCCCTCCTTTATAAAGTCCTGGCCTCGGGACAGCCCGCACAGCTGCCCAGCCTGCGGAGACGGGACA
GCCCTGTCCCACTCACTCTTTCCCCTGCTGCTCCTGCCGGCAGCTCAGCTGGAACCATGGGAGGCCGCGTCTTTCTCGTCTTTCTCGCATTCTGTGTCTG
GCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGGACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAAT
CCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCCATGGAGACTTGTGACGACATCAACGAGTGTGCAACACTGTCGAAAGTGTCATGCGGAAAAT
TCTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCAGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAA
CACGTGTCAAGATGTGGACGAATGTCAGCAGAACCCAAGGCTCTGTAAAAGCTACGGCACCTGCGTCAACACCCTCGGCAGCTACACGTGCCAGTGCCTG
CCTGGCTTCAAGCTCAAACCTGAGGACCCGAAGCTCTGCACAGATGTGGACGAGTGCAGCTCCGGGCAGCATCAGTGTGACAGCTCCACCGTCTGCTTCA
ACACCGTGGGTTCATACAGCTGCCGCTGCCGCCCAGGCTGGAAGCCCAGACACGGAATCCCGAATAACCAAAAGGACACTGTCTGTGAAGATATGACTTT
CTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGATTCTTCGACAAAGTCCAGGACCTGGGCAGAGACTACAAGCCAGGCTTGGCC
AATAACACCATCCAGAGCATCTTACAGGCGCTGGATGAGCTGCTGGAGGCCCCTGGGGACCTGGAGACCCTGCCCCGCTTACAGCAGCACTGTGTGGCCA
GTCACCTGCTGGATGGCCTAGAGGATGTCCTCAGAGGCCTGAGCAAGAACCTTTCCAATGGGCTGTTGAACTTCAGTTATCCTGCAGGCACAGAATTGTC
CCTGGAGGTGCAGAAGCAAGTAGACAGGAGTGTCACCTTGAGACAGAATCAGGCAGTGATGCAGCTCGACTGGAATCAGGCACAGAAATCTGGTGACCCA
GGCCCTTCTGTGGTGGGCCTTGTCTCCATTCCAGGGATGGGCAAGTTGCTGGCTGAGGCCCCTCTGGTCCTGGAACCTGAGAAGCAGATGCTTCTGCATG
AGACACACCAGGGCTTGCTGCAGGACGGCTCCCCCATCCTGCTCTCAGATGTGATCTCTGCCTTTCTGAGCAACAACGACACCCAAAACCTCAGCTCCCC
AGTTACCTTCACCTTCTCCCACCGTTCAGTGATCCCGAGACAGAAGGTGCTCTGTGTCTTCTGGGAGCATGGCCAGAATGGATGTGGTCACTGGGCCACC
ACAGGCTGCAGCACAATAGGCACCAGAGACACCAGCACCATCTGCCGTTGCACCCACCTGAGCAGCTTTGCCGTCCTCATGGCCCACTACGATGTGCAGG
AGGAGGATCCCGTGCTGACTGTCATCACCTACATGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTCCTGGCGGCCCTCACTTTTCTCCTGTGTAAAGCCAT
CCAGAACACCAGCACCTCACTGCATCTGCAGCTCTCGCTCTGCCTCTTCCTGGCCCACCTCCTCTTCCTCGTGGCAATTGATCAAACCGGACACAAGGTG
CTGTGCTCCATCATCGCCGGTACCTTGCACTATCTCTACCTGGCCACCTTGACCTGGATGCTGCTGGAGGCCCTGTACCTCTTCCTCACTGCACGGAACC
TGACGGTGGTCAACTACTCAAGCATCAACAGATTCATGAAGAAGCTCATGTTCCCTGTGGGCTACGGAGTCCCAGCTGTGACAGTGGCCATTTCTGCAGC
CTCCAGGCCTCACCTTTATGGAACACCTTCCCGCTGCTGGCTCCAACCAGAAAAGGGATTTATATGGGGCTTCCTTGGACCTGTCTGCGCCATCTTCTCT
GTGAATTTAGTTCTCTTTCTGGTGACTCTCTGGATTTTGAAAAACAGACTCTCCTCCCTCAATAGTGAAGTGTCCACCCTCCGGAACACAAGGATGCTGG
CATTTAAAGCGACAGCTCAGCTGTTCATCCTGGGCTGCACGTGGTGTCTGGGCATCTTGCAGGTGGGTCCGGCTGCCCGGGTCATGGCCTACCTCTTCAC
CATCATCAACAGCCTGCAGGGTGTCTTCATCTTCCTGGTGTACTGCCTCCTCAGCCAGCAGGTCCGGGAGCAATATGGGAAATGGTCCAAAGGGATCAGG
AAATTGAAAACTGAGTCTGAGATGCACACACTCTCCAGCAGTGCTAAGGCTGACACCTCCAAACCCAGCACGGTTAACTAGAAAAATCTTCTGAATAAGA
TCTTCCCTCTTTGCCCGTGGAAAATCTGAACAATCTTTGAGCCATCTAGAGGGGAAAGAAAAGACTTTGTTCTGTGTGTTTCAAGAAATTCACCATGTCA
GCAATATGAAGGATGTTATGGAAGGCGTGCTAGGCATTCAATTCCTGCAGAAACCGGAAATCTTCCATGCCCTGCAATGTGCTCATCAAACTCTCAGCAT
ATGGACGGCCAGCTGTGGCCCATATCTTGGTCACTCTGAAGCACAATATTTATGAAGCTATAGAACGTTAAGACCTCTTTCACAGCCTCTCCTTCCTACA
AAGACTCCTCCAAATCTTAAAATGAAGCAGGAAAACGAGCCTAAGAGGACTTTCATACCGACAACATCTGAAAGGACTAGAATGTTCACACCACGATCTG
GATTTCTTAATTTTTTGTTTTTGTTTTTGTTGTTCTCTAGTTCTACGGGTTTGATTATTTAGTCATGTGAAAAATATTGATTACTCACACATAGATCAAG
AGAGACACGGCTCCTGCCTTCATGGAGCTTTTAGGGGAAAATGAAGTGGCTCTTGCAGCTAGAGTTGACTCAGAAGCCGAAATTCCTAGAAATCAGGTTT
CTACTGCTAGGCAATTGAAGTATAAACTATTTTATAAACACTGTCTTCTTTCGTCTTCACACCAACATGCAGAAAAGTTTCTAATCTCAGATCGGGGATG
TGCAACAAATTCCATTTCAAAGGAATGACCTGCAAAACTCCTAAATATTCCAAGCAAATGCCCTTAACCCTGTCTGTTATCTGCTTTCCTTGAACAGAAA
TTCTACATGACCATAAAACCTCGAAGATGGGTATGGCACAGTTCATGCCCTGTAATCCTAGCACTTTGGGAGGGTGAGGCAGGAGGATGGCTCAAGCCCA
GGAGTTTGAGACCAGTGTGGGCAACAGAGTGAGAACCATCTCTACCCAAAAAAAAAATTAAAAATTAGCCAAGCATGGTGATGATATAGGAGTTAAGGAG
AAATCATTTAGGCAAATAGCAAGGGTAGGAAGTCCTCAGTAAGGTTTTCCATTTAATGAAAAGCAGCCCCCAAAATCATTTTCTTTTCTAACAAAGAACA
GCCTGTAAAATCGAGCTGCAGACATAGACAAGCAAGCTGGAAGCTTCCACGGGTGAATGCCGGCAGCTGTGCCAATAGGAAAAAGCTACCTAGACTAGGC
ATGTCCAAAATGGCGGCTCCAAGTTCCCTTCTCTTTGCCAGCCATGTGTACAGTAAAAAGCAGGCAACATAGTGTCAGCCAAAGCTCATTTGCATAATAA
GATTAGGGTGGGGTGGCCAGCTCACATAGGGGTAGGCCCTAGGTAAATCAGACACCGCCTTCTCAAGCCTGTCTATAAAATCTGGTACACTATGACGAGG
GTCAGATTTCCCATTCAGACGCCCCTCTCCCATGCAAGAGAAAGAGCTGTTCTCCTTTCTCTTTCTTTTGCCTATTAAACCTCTGCTCCTGGCCAGGCAC
AGTGGCTCACGCCTATAATCCCAGCACTTTGGGAGGCTGAGGTGGTCAGATCACCTAAGGTCAGGAGTTCAAGACCAGCCTGGTCAACATGGTGAAATCT
TGTCTCTAGTAAAAATACAAAAATATATGAAATCTCACATAGATGATAATATTAAGTTCCAAAAGCAACTCAACCTGGTAGATTCTAATTTTTTTTGAGG
CAGGGTCTTGCTTTGTCACCCATGCTGGAGTACAATGGCACAAACACTGCTCACTGCAGCCTCGACCTCCCAAGGCCTAAGCAATCCTCCTGCCTCAGTC
CCCCTCCAGGTATTTGAAACTACAGGTGTGTACCACCACACCGGGCTAATTTTTGTATTTTTTGTAGAGACGTGGGTCTCACTATGCTGCCCAGGCTCAG
GTCTTAATCTCCTGAGCTCAGGCAATCCGCAGGCCTCAGCCTCCCTAAGTGCGGGGATTACAGGCTTGAGCCACTGCACCTAGCCTCTATTTGTTTTACA
AAAGAGAAATTGAGATCCTGAATGTTAAGTGACTTGCCTGAGGCCATCCCACTAACAGGAGCCAGGGTTAGGATTCAAACCCCATCCAACTGGTCCCAGA
GCTGGAGCTTCTTGCACTGCCCTACACTACCTACCATCTCCATCCTCTGGGCACCTTTTTATAAGAACCAAAACATTACAGAGCATTGCTTTGTCAACTC
AGCTGGGAACATTTCCCAGTGCAACTCACATTTTTCACTGCTCTGTGCCTGTCCGTATAAGCTCAATGAGTATTGATTTAGGGGCTTTGGAGAACTTTGA
ATGCTACCCCCCAAGTAACCATTGTTGGCAACCTGGTACCTCTACTTTTAGCCATTTCTCCTTCTCTATAAATAGTGCAGAAGTAACCCACTTGGTAACA
GGCATCCTTGCCAAGCCTCCACCACTAGGTCAGTGTAAGAATTAAAGAAAGAGGAAAGAAACACAAAAAGTGGCTTGATGGTTAAGACAGGTTTATTTTA
GAGAAAACACACCTGAGAGGGGCTGCTGGCTGAATTAGGTTAGAGTCTTTTCTACAGACTAAGAGTGTTTAAGGATTTAGGGTGGGAGAGTTTCTTAGAG
GCTTGGACTGCTTCTGTGTTTTTTTTGTTGTGCTTATATGGGAGGGAGAGTGGTGTGTTTGCTTTTATACATTTTTCTGCAGCTGTAGGCATACCCCCCA
AGTCTGCTTTTAGCTTCCCTATTTTAGTGCACCTGGAGGGAAAGGAATGTGCTTATTAAGGCCCACTGTTTTACTGGGGCCCATTGTATGAGGGTGAAGT
TTGGCAGTTACCCAAGAGACTTTTCCTCCACCTTCCTCTGTGCCCGAGCTGTTTTATCTGCATTTTACTGTCTGCTTTTTTTGGCTGCTTATAGTTTTTA
AAAAAGTAATTTCCTTAAATCCAGAAGGCTAAAAATGAAGCTGAAACTTAAAGTGGCGGTGTTTGTCCAAAATAACGGGGCTCCTGCTCTGCCAGTCAGT
ACCCTCAAGTCACTCCTGATCCTCAACCTCCATGCCTAAGGCTGGTTCAAGAGACCACATAATATCTGCCTTTTATTACATACATGATGGGTGCATGGGA
TTCTGCGTGCCCTTTGCTTGATATAGACTGCTAAGGTGAGATGGGGAATATCAGAGTCAGCTGCTGCTTGAGGAAGCAGAACACACAGCTGGAGGCTTGG
AACATGTGGGTCCCTATGAGTGTAGAGCCCATATCCCCATAGAGTCTACCTAGAGCAGGGGTCGCCAAATGTTTTCTTAAAGAGCCTGATAGTGTATATG
TTAGGCTTTGTGAGCCAGGTATTTACAGCAACTCAATTCTACCACTGTGGTATGAAAACAGCTATAGACAATCATAAATGAATGATCATGGCTATGTTTT
AATAAAACTTTACAGACACTGAACTTGAACTTCCATTGTGATATGAAAACAGCTATAGACAATCATAAATGAATGATCATGGCTATGTTTTAATAAAACT
TTATGGACACTGAGCTTGAACTTCATATATCAGTCATGTGACACAAAATATCATTCTTCTTTTTATTTGTTTTCACCCATTTGAAAAATGTAAAAACTAT
TCTTAGCTGTACAGAAACAGATGGTGGGTCAGATTTGACTCACTGCCCATAGTTTCCAGACCATGATGTTCAGGTTCATGGAAGCACTTCACTCTACACT
AATTTATTCATCCATTCATCACATATGTGATGAGCACATACAGACACACCTCAGAGATATTGCAGGCTCCACTCCAGATCACCACAATAATGTGAATATT
GCAATAAAGCAAGTCATACACATT
- refseq_EMR2.F3 (forward primer)
- refseq_EMR2.R3 (reverse primer)
- Alternative acceptor
Frame 0
GGSALCGEASLLGTCLASEEGPFSLPGLLCGHC
NASVFFLSFCF*HRVSLYHPSWSAVAPS*LTAAS
NSPARVILPASAS*VVAAGTTDVCHQAQLHRQF
RGGPWASGCPPPLLYKVLASGQPAQLPSLRRRD
SPVPLTLSPAAPAGSSAGTMGGRVFLVFLAFCVW
LTLPGAETQDSRGCARWCPQDSSCVNATACRCN
PGFSSFSEIITTPMETCDDINECATLSKVSCGK
FSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESEN
TCQDVDECQQNPRLCKSYGTCVNTLGSYTCQCL
PGFKLKPEDPKLCTDVDECSSGQHQCDSSTVCF
NTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMTF
STWTPPPGVHSQTLSRFFDKVQDLGRDYKPGLA
NNTIQSILQALDELLEAPGDLETLPRLQQHCVA
SHLLDGLEDVLRGLSKNLSNGLLNFSYPAGTELS
LEVQKQVDRSVTLRQNQAVMQLDWNQAQKSGDP
GPSVVGLVSIPGMGKLLAEAPLVLEPEKQMLLH
ETHQGLLQDGSPILLSDVISAFLSNNDTQNLSSP
VTFTFSHRSVIPRQKVLCVFWEHGQNGCGHWAT
TGCSTIGTRDTSTICRCTHLSSFAVLMAHYDVQ
EEDPVLTVITYMGLSVSLLCLLLAALTFLLCKAI
QNTSTSLHLQLSLCLFLAHLLFLVAIDQTGHKV
LCSIIAGTLHYLYLATLTWMLLEALYLFLTARN
LTVVNYSSINRFMKKLMFPVGYGVPAVTVAISAA
SRPHLYGTPSRCWLQPEKGFIWGFLGPVCAIFS
VNLVLFLVTLWILKNRLSSLNSEVSTLRNTRML
AFKATAQLFILGCTWCLGILQVGPAARVMAYLFT
IINSLQGVFIFLVYCLLSQQVREQYGKWSKGIR
KLKTESEMHTLSSSAKADTSKPSTVN*KNLLNK
IFPLCPWKI*TIFEPSRGERKDFVLCVSRNSPCQ
QYEGCYGRRARHSIPAETGNLPCPAMCSSNSQH
MDGQLWPISWSL*STIFMKL*NVKTSFTASPSY
KDSSKS*NEAGKRA*EDFHTDNI*KD*NVHTTIW
IS*FFVFVFVVL*FYGFDYLVM*KILITHT*IK
RDTAPAFMELLGENEVALAARVDSEAEIPRNQV
STARQLKYKLFYKHCLLSSSHQHAEKFLISDRGC
ATNSISKE*PAKLLNIPSKCP*PCLLSAFLEQK
FYMTIKPRRWVWHSSCPVILALWEGEAGGWLKP
RSLRPVWATE*EPSLPKKKIKN*PSMVMI*ELRR
NHLGK*QG*EVLSKVFHLMKSSPQNHFLF*QRT
ACKIELQT*TSKLEASTGECRQLCQ*EKAT*TR
HVQNGGSKFPSLCQPCVQ*KAGNIVSAKAHLHNK
IRVGWPAHIGVGPR*IRHRLLKPVYKIWYTMTR
VRFPIQTPLSHARERAVLLSLSFAY*TSAPGQA
QWLTPIIPALWEAEVVRSPKVRSSRPAWSTW*NL
VSSKNTKIYEISHR**Y*VPKATQPGRF*FFLR
QGLALSPMLEYNGTNTAHCSLDLPRPKQSSCLS
PPPGI*NYRCVPPHRANFCIFCRDVGLTMLPRLR
S*SPELRQSAGLSLPKCGDYRLEPLHLASICFT
KEKLRS*MLSDLPEAIPLTGARVRIQTPSNWSQ
SWSFLHCPTLPTISILWAPFYKNQNITEHCFVNS
AGNISQCNSHFSLLCACPYKLNEY*FRGFGEL*
MLPPK*PLLATWYLYF*PFLLLYK*CRSNPLGN
RHPCQASTTRSV*ELKKEERNTKSGLMVKTGLF*
RKHT*EGLLAELG*SLFYRLRVFKDLGWESFLE
AWTASVFFLLCLYGRESGVFAFIHFSAAVGIPP
KSAFSFPILVHLEGKECAY*GPLFYWGPLYEGEV
WQLPKRLFLHLPLCPSCFICILLSAFFGCL*FL
KK*FP*IQKAKNEAET*SGGVCPK*RGSCSASQ
YPQVTPDPQPPCLRLVQETT*YLPFITYMMGAWD
SACPLLDIDC*GEMGNIRVSCCLRKQNTQLEAW
NMWVPMSVEPISP*SLPRAGVAKCFLKEPDSVY
VRLCEPGIYSNSILPLWYENSYRQS*MNDHGYVL
IKLYRH*T*TSIVI*KQL*TIINE*SWLCFNKT
LWTLSLNFIYQSCDTKYHSSFYLFSPI*KM*KL
FLAVQKQMVGQI*LTAHSFQTMMFRFMEALHSTL
IYSSIHHICDEHIQTHLRDIAGSTPDHHNNVNI
AIKQVIHI
Frame 1
GGLLCAVKLLFLAPAWHRKRAPSPSLGFYVDTV
MPQFSFFLFVFDTGSHSITQAGVQWHHLSSPQP
QTPQLG*FFLPQPPE**QLGPQTSATKPSCTASS
GEGPGPAAVRPPSFIKSWPRDSPHSCPACGDGT
ALSHSLFPLLLLPAAQLEPWEAASFSSFSHSVS
G*LCRELKPRTPGAVPGGALRTPRVSMPPPVAAI
QGSALFLRSSPPPWRLVTTSTSVQHCRKCHAEN
SRTAGTQRGATTACAAQDMSLFLGQKHSRMRAR
TRVKMWTNVSRTQGSVKATAPASTPSAATRASAC
LASSSNLRTRSSAQMWTSAAPGSISVTAPPSAS
TPWVHTAAAAAQAGSPDTESRITKRTLSVKI*L
SPPGPRPLESTARRFPDSSTKSRTWAETTSQAWP
ITPSRASYRRWMSCWRPLGTWRPCPAYSSTVWP
VTCWMA*RMSSEA*ARTFPMGC*TSVILQAQNC
PWRCRSK*TGVSP*DRIRQ*CSSTGIRHRNLVTQ
ALLWWALSPFQGWASCWLRPLWSWNLRSRCFCM
RHTRACCRTAPPSCSQM*SLPF*ATTTPKTSAP
QLPSPSPTVQ*SRDRRCSVSSGSMARMDVVTGPP
QAAAQ*APETPAPSAVAPT*AALPSSWPTTMCR
RRIPC*LSSPTWG*ASLCCASSWRPSLFSCVKP
SRTPAPHCICSSRSASSWPTSSSSWQLIKPDTRC
CAPSSPVPCTISTWPP*PGCCWRPCTSSSLHGT
*RWSTTQASTDS*RSSCSLWATESQL*QWPFLQ
PPGLTFMEHLPAAGSNQKRDLYGASLDLSAPSSL
*I*FSFW*LSGF*KTDSPPSIVKCPPSGTQGCW
HLKRQLSCSSWAARGVWASCRWVRLPGSWPTSS
PSSTACRVSSSSWCTASSASRSGSNMGNGPKGSG
N*KLSLRCTHSPAVLRLTPPNPARLTRKIF*IR
SSLFARGKSEQSLSHLEGKEKTLFCVFQEIHHV
SNMKDVMEGVLGIQFLQKPEIFHALQCAHQTLSI
WTASCGPYLGHSEAQYL*SYRTLRPLSQPLLPT
KTPPNLKMKQENEPKRTFIPTTSERTRMFTPRS
GFLNFLFLFLLFSSSTGLII*SCEKY*LLTHRSR
ETRLLPSWSF*GKMKWLLQLELTQKPKFLEIRF
LLLGN*SINYFINTVFFRLHTNMQKSF*SQIGD
VQQIPFQRNDLQNS*IFQANALNPVCYLLSLNRN
ST*P*NLEDGYGTVHAL*S*HFGRVRQEDGSSP
GV*DQCGQQSENHLYPKKKLKISQAW**YRS*G
EII*ANSKGRKSSVRFSI**KAAPKIIFFSNKEQ
PVKSSCRHRQASWKLPRVNAGSCANRKKLPRLG
MSKMAAPSSLLFASHVYSKKQAT*CQPKLICII
RLGWGGQLT*G*ALGKSDTAFSSLSIKSGTL*RG
SDFPFRRPSPMQEKELFSFLFLLPIKPLLLARH
SGSRL*SQHFGRLRWSDHLRSGVQDQPGQHGEI
LSLVKIQKYMKSHIDDNIKFQKQLNLVDSNFF*G
RVLLCHPCWSTMAQTLLTAASTSQGLSNPPASV
PLQVFETTGVYHHTGLIFVFFVETWVSLCCPGS
GLNLLSSGNPQASASLSAGITGLSHCT*PLFVLQ
KRN*DPEC*VTCLRPSH*QEPGLGFKPHPTGPR
AGASCTALHYLPSPSSGHLFIRTKTLQSIALST
QLGTFPSATHIFHCSVPVRISSMSIDLGALENFE
CYPPSNHCWQPGTSTFSHFSFSINSAEVTHLVT
GILAKPPPLGQCKN*RKRKETQKVA*WLRQVYF
RENTPERGCWLN*VRVFSTD*ECLRI*GGRVS*R
LGLLLCFFCCAYMGGRVVCLLLYIFLQL*AYPP
SLLLASLF*CTWRERNVLIKAHCFTGAHCMRVK
FGSYPRDFSSTFLCARAVLSAFYCLLFLAAYSF*
KSNFLKSRRLKMKLKLKVAVFVQNNGAPALPVS
TLKSLLILNLHA*GWFKRPHNICLLLHT*WVHG
ILRALCLI*TAKVRWGISESAAA*GSRTHSWRLG
TCGSL*V*SPYPHRVYLEQGSPNVFLKSLIVYM
LGFVSQVFTATQFYHCGMKTAIDNHK*MIMAMF
**NFTDTELELPL*YENSYRQS*MNDHGYVLIKL
YGH*A*TSYISHVTQNIILLFICFHPFEKCKNY
S*LYRNRWWVRFDSLPIVSRP*CSGSWKHFTLH
*FIHPFITYVMSTYRHTSEILQAPLQITTIM*IL
Q*SKSYT
Frame 2
GVCSVR*SFSSWHLPGIGRGPLLPPWAFMWTL*
CLSFLSFFLFLTQGLTLSPKLECSGTILAHRSL
KLPSSGDSSCLSLLSSSSWDHRRLPPSPAAPPV
PGRALGQRLSAPPPL*SPGLGTARTAAQPAETGQ
PCPTHSFPCCSCRQLSWNHGRPRLSRLSRILCL
ADSAGS*NPGLQGLCPVVPSGLLVCQCHRLSLQ
SRVQLFF*DHHHPHGDL*RHQRVCNTVESVMRKI
LGLLEHRGELRLRVQPRI*ACFWGKNIQE*ERE
HVSRCGRMSAEPKAL*KLRHLRQHPRQLHVPVP
AWLQAQT*GPEALHRCGRVQLRAASV*QLHRLLQ
HRGFIQLPLPPRLEAQTRNPE*PKGHCL*RYDF
LHLDPAPWSPQPDAFPILRQSPGPGQRLQARLG
Q*HHPEHLTGAG*AAGGPWGPGDPAPLTAALCGQ
SPAGWPRGCPQRPEQEPFQWAVELQLSCRHRIV
PGGAEASRQECHLETESGSDAARLESGTEIW*P
RPFCGGPCLHSRDGQVAG*GPSGPGT*EADASA*
DTPGLAAGRLPHPALRCDLCLSEQQRHPKPQLP
SYLHLLPPFSDPETEGALCLLGAWPEWMWSLGH
HRLQHNRHQRHQHHLPLHPPEQLCRPHGPLRCAG
GGSRADCHHLHGAERLSAVPPPGGPHFSPV*SH
PEHQHLTASAALALPLPGPPPLPRGN*SNRTQG
AVLHHRRYLALSLPGHLDLDAAGGPVPLPHCTEP
DGGQLLKHQQIHEEAHVPCGLRSPSCDSGHFCS
LQASPLWNTFPLLAPTRKGIYMGLPWTCLRHLL
CEFSSLSGDSLDFEKQTLLPQ**SVHPPEHKDAG
I*SDSSAVHPGLHVVSGHLAGGSGCPGHGLPLH
HHQQPAGCLHLPGVLPPQPAGPGAIWEMVQRDQ
EIEN*V*DAHTLQQC*G*HLQTQHG*LEKSSE*D
LPSLPVENLNNL*AI*RGKKRLCSVCFKKFTMS
AI*RMLWKAC*AFNSCRNRKSSMPCNVLIKLSA
YGRPAVAHILVTLKHNIYEAIER*DLFHSLSFLQ
RLLQILK*SRKTSLRGLSYRQHLKGLECSHHDL
DFLIFCFCFCCSLVLRV*LFSHVKNIDYSHIDQ
ERHGSCLHGAFRGK*SGSCS*S*LRSRNS*KSGF
YC*AIEV*TIL*TLSSFVFTPTCRKVSNLRSGM
CNKFHFKGMTCKTPKYSKQMPLTLSVICFP*TE
ILHDHKTSKMGMAQFMPCNPSTLGG*GRRMAQAQ
EFETSVGNRVRTISTQKKN*KLAKHGDDIGVKE
KSFRQIARVGSPQ*GFPFNEKQPPKSFSFLTKN
SL*NRAADIDKQAGSFHG*MPAAVPIGKSYLD*A
CPKWRLQVPFSLPAMCTVKSRQHSVSQSSFA**
D*GGVASSHRGRP*VNQTPPSQACL*NLVHYDE
GQISHSDAPLPCKRKSCSPFSFFCLLNLCSWPGT
VAHAYNPSTLGG*GGQIT*GQEFKTSLVNMVKS
CL**KYKNI*NLT*MIILSSKSNSTW*ILIFFE
AGSCFVTHAGVQWHKHCSLQPRPPKA*AILLPQS
PSRYLKLQVCTTTPG*FLYFL*RRGSHYAAQAQ
VLIS*AQAIRRPQPP*VRGLQA*ATAPSLYLFY
KREIEILNVK*LA*GHPTNRSQG*DSNPIQLVPE
LELLALPYTTYHLHPLGTFL*EPKHYRALLCQL
SWEHFPVQLTFFTALCLSV*AQ*VLI*GLWRTL
NATPQVTIVGNLVPLLLAISPSL*IVQK*PTW*Q
ASLPSLHH*VSVRIKERGKKHKKWLDG*DRFIL
EKTHLRGAAG*IRLESFLQTKSV*GFRVGEFLR
GLDCFCVFFVVLIWEGEWCVCFYTFFCSCRHTPQ
VCF*LPYFSAPGGKGMCLLRPTVLLGPIV*G*S
LAVTQETFPPPSSVPELFYLHFTVCFFWLLIVF
KKVISLNPEG*K*S*NLKWRCLSKITGLLLCQSV
PSSHS*SSTSMPKAGSRDHIISAFYYIHDGCMG
FCVPFA*YRLLR*DGEYQSQLLLEEAEHTAGGL
EHVGPYECRAHIPIEST*SRGRQMFS*RA**CIC
*AL*ARYLQQLNSTTVV*KQL*TIINE*SWLCF
NKTLQTLNLNFHCDMKTAIDNHK*MIMAMF**N
FMDTELELHISVM*HKISFFFLFVFTHLKNVKTI
LSCTETDGGSDLTHCP*FPDHDVQVHGSTSLYT
NLFIHSSHM**AHTDTPQRYCRLHSRSPQ*CEY
CNKASHTH
Sequence NM_152918
GGGGGGTCTGCTCTGTGCGGTGAAGCTTCTCTTCTTGGCACCTGCCTGGCATCGGAAGAGGGCCCCTTCTCCCTCCCTGGGCTTTTATGTGGACACTGTA
ATGCCTCAGTTTTCTTTCTTTCTTTTTGTTTTTGACACAGGGTCTCACTCTATCACCCAAGCTGGAGTGCAGTGGCACCATCTTAGCTCACCGCAGCCTC
AAACTCCCCAGCTCGGGTGATTCTTCCTGCCTCAGCCTCCTGAGTAGTAGCAGCTGGGACCACAGACGTCTGCCACCAAGCCCAGCTGCACCGCCAGTTC
CGGGGAGGGCCCTGGGCCAGCGGCTGTCCGCCCCCCCTCCTTTATAAAGTCCTGGCCTCGGGACAGCCCGCACAGCTGCCCAGCCTGCGGAGACGGGACA
GCCCTGTCCCACTCACTCTTTCCCCTGCTGCTCCTGCCGGCAGCTCAGCTGGAACCATGGGAGGCCGCGTCTTTCTCGTCTTTCTCGCATTCTGTGTCTG
GCTGACTCTGCCGGGAGCTGAAACCCAGGACTCCAGGGGCTGTGCCCGGTGGTGCCCTCAGGACTCCTCGTGTGTCAATGCCACCGCCTGTCGCTGCAAT
CCAGGGTTCAGCTCTTTTTCTGAGATCATCACCACCCCCATGGAGACTTGTGACGACATCAACGAGTGTGCAACACTGTCGAAAGTGTCATGCGGAAAAT
TCTCGGACTGCTGGAACACAGAGGGGAGCTACGACTGCGTGTGCAGCCCAGGATATGAGCCTGTTTCTGGGGCAAAAACATTCAAGAATGAGAGCGAGAA
CACGTGTCAAGATATGACTTTCTCCACCTGGACCCCGCCCCCTGGAGTCCACAGCCAGACGCTTTCCCGATTCTTCGACAAAGTCCAGGACCTGGGCAGA
GACTACAAGCCAGGCTTGGCCAATAACACCATCCAGAGCATCTTACAGGCGCTGGATGAGCTGCTGGAGGCCCCTGGGGACCTGGAGACCCTGCCCCGCT
TACAGCAGCACTGTGTGGCCAGTCACCTGCTGGATGGCCTAGAGGATGTCCTCAGAGGCCTGAGCAAGAACCTTTCCAATGGGCTGTTGAACTTCAGTTA
TCCTGCAGGCACAGAATTGTCCCTGGAGGTGCAGAAGCAAGTAGACAGGAGTGTCACCTTGAGACAGAATCAGGCAGTGATGCAGCTCGACTGGAATCAG
GCACAGAAATCTGGTGACCCAGGCCCTTCTGTGGTGGGCCTTGTCTCCATTCCAGGGATGGGCAAGTTGCTGGCTGAGGCCCCTCTGGTCCTGGAACCTG
AGAAGCAGATGCTTCTGCATGAGACACACCAGGGCTTGCTGCAGGACGGCTCCCCCATCCTGCTCTCAGATGTGATCTCTGCCTTTCTGAGCAACAACGA
CACCCAAAACCTCAGCTCCCCAGTTACCTTCACCTTCTCCCACCGTTCAGTGATCCCGAGACAGAAGGTGCTCTGTGTCTTCTGGGAGCATGGCCAGAAT
GGATGTGGTCACTGGGCCACCACAGGCTGCAGCACAATAGGCACCAGAGACACCAGCACCATCTGCCGTTGCACCCACCTGAGCAGCTTTGCCGTCCTCA
TGGCCCACTACGATGTGCAGGAGGAGGATCCCGTGCTGACTGTCATCACCTACATGGGGCTGAGCGTCTCTCTGCTGTGCCTCCTCCTGGCGGCCCTCAC
TTTTCTCCTGTGTAAAGCCATCCAGAACACCAGCACCTCACTGCATCTGCAGCTCTCGCTCTGCCTCTTCCTGGCCCACCTCCTCTTCCTCGTGGCAATT
GATCAAACCGGACACAAGGTGCTGTGCTCCATCATCGCCGGTACCTTGCACTATCTCTACCTGGCCACCTTGACCTGGATGCTGCTGGAGGCCCTGTACC
TCTTCCTCACTGCACGGAACCTGACGGTGGTCAACTACTCAAGCATCAACAGATTCATGAAGAAGCTCATGTTCCCTGTGGGCTACGGAGTCCCAGCTGT
GACAGTGGCCATTTCTGCAGCCTCCAGGCCTCACCTTTATGGAACACCTTCCCGCTGCTGGCTCCAACCAGAAAAGGGATTTATATGGGGCTTCCTTGGA
CCTGTCTGCGCCATCTTCTCTGTGAATTTAGTTCTCTTTCTGGTGACTCTCTGGATTTTGAAAAACAGACTCTCCTCCCTCAATAGTGAAGTGTCCACCC
TCCGGAACACAAGGATGCTGGCATTTAAAGCGACAGCTCAGCTGTTCATCCTGGGCTGCACGTGGTGTCTGGGCATCTTGCAGGTGGGTCCGGCTGCCCG
GGTCATGGCCTACCTCTTCACCATCATCAACAGCCTGCAGGGTGTCTTCATCTTCCTGGTGTACTGCCTCCTCAGCCAGCAGGTCCGGGAGCAATATGGG
AAATGGTCCAAAGGGATCAGGAAATTGAAAACTGAGTCTGAGATGCACACACTCTCCAGCAGTGCTAAGGCTGACACCTCCAAACCCAGCACGGTTAACT
AGAAAAATCTTCTGAATAAGATCTTCCCTCTTTGCCCGTGGAAAATCTGAACAATCTTTGAGCCATCTAGAGGGGAAAGAAAAGACTTTGTTCTGTGTGT
TTCAAGAAATTCACCATGTCAGCAATATGAAGGATGTTATGGAAGGCGTGCTAGGCATTCAATTCCTGCAGAAACCGGAAATCTTCCATGCCCTGCAATG
TGCTCATCAAACTCTCAGCATATGGACGGCCAGCTGTGGCCCATATCTTGGTCACTCTGAAGCACAATATTTATGAAGCTATAGAACGTTAAGACCTCTT
TCACAGCCTCTCCTTCCTACAAAGACTCCTCCAAATCTTAAAATGAAGCAGGAAAACGAGCCTAAGAGGACTTTCATACCGACAACATCTGAAAGGACTA
GAATGTTCACACCACGATCTGGATTTCTTAATTTTTTGTTTTTGTTTTTGTTGTTCTCTAGTTCTACGGGTTTGATTATTTAGTCATGTGAAAAATATTG
ATTACTCACACATAGATCAAGAGAGACACGGCTCCTGCCTTCATGGAGCTTTTAGGGGAAAATGAAGTGGCTCTTGCAGCTAGAGTTGACTCAGAAGCCG
AAATTCCTAGAAATCAGGTTTCTACTGCTAGGCAATTGAAGTATAAACTATTTTATAAACACTGTCTTCTTTCGTCTTCACACCAACATGCAGAAAAGTT
TCTAATCTCAGATCGGGGATGTGCAACAAATTCCATTTCAAAGGAATGACCTGCAAAACTCCTAAATATTCCAAGCAAATGCCCTTAACCCTGTCTGTTA
TCTGCTTTCCTTGAACAGAAATTCTACATGACCATAAAACCTCGAAGATGGGTATGGCACAGTTCATGCCCTGTAATCCTAGCACTTTGGGAGGGTGAGG
CAGGAGGATGGCTCAAGCCCAGGAGTTTGAGACCAGTGTGGGCAACAGAGTGAGAACCATCTCTACCCAAAAAAAAAATTAAAAATTAGCCAAGCATGGT
GATGATATAGGAGTTAAGGAGAAATCATTTAGGCAAATAGCAAGGGTAGGAAGTCCTCAGTAAGGTTTTCCATTTAATGAAAAGCAGCCCCCAAAATCAT
TTTCTTTTCTAACAAAGAACAGCCTGTAAAATCGAGCTGCAGACATAGACAAGCAAGCTGGAAGCTTCCACGGGTGAATGCCGGCAGCTGTGCCAATAGG
AAAAAGCTACCTAGACTAGGCATGTCCAAAATGGCGGCTCCAAGTTCCCTTCTCTTTGCCAGCCATGTGTACAGTAAAAAGCAGGCAACATAGTGTCAGC
CAAAGCTCATTTGCATAATAAGATTAGGGTGGGGTGGCCAGCTCACATAGGGGTAGGCCCTAGGTAAATCAGACACCGCCTTCTCAAGCCTGTCTATAAA
ATCTGGTACACTATGACGAGGGTCAGATTTCCCATTCAGACGCCCCTCTCCCATGCAAGAGAAAGAGCTGTTCTCCTTTCTCTTTCTTTTGCCTATTAAA
CCTCTGCTCCTGGCCAGGCACAGTGGCTCACGCCTATAATCCCAGCACTTTGGGAGGCTGAGGTGGTCAGATCACCTAAGGTCAGGAGTTCAAGACCAGC
CTGGTCAACATGGTGAAATCTTGTCTCTAGTAAAAATACAAAAATATATGAAATCTCACATAGATGATAATATTAAGTTCCAAAAGCAACTCAACCTGGT
AGATTCTAATTTTTTTTGAGGCAGGGTCTTGCTTTGTCACCCATGCTGGAGTACAATGGCACAAACACTGCTCACTGCAGCCTCGACCTCCCAAGGCCTA
AGCAATCCTCCTGCCTCAGTCCCCCTCCAGGTATTTGAAACTACAGGTGTGTACCACCACACCGGGCTAATTTTTGTATTTTTTGTAGAGACGTGGGTCT
CACTATGCTGCCCAGGCTCAGGTCTTAATCTCCTGAGCTCAGGCAATCCGCAGGCCTCAGCCTCCCTAAGTGCGGGGATTACAGGCTTGAGCCACTGCAC
CTAGCCTCTATTTGTTTTACAAAAGAGAAATTGAGATCCTGAATGTTAAGTGACTTGCCTGAGGCCATCCCACTAACAGGAGCCAGGGTTAGGATTCAAA
CCCCATCCAACTGGTCCCAGAGCTGGAGCTTCTTGCACTGCCCTACACTACCTACCATCTCCATCCTCTGGGCACCTTTTTATAAGAACCAAAACATTAC
AGAGCATTGCTTTGTCAACTCAGCTGGGAACATTTCCCAGTGCAACTCACATTTTTCACTGCTCTGTGCCTGTCCGTATAAGCTCAATGAGTATTGATTT
AGGGGCTTTGGAGAACTTTGAATGCTACCCCCCAAGTAACCATTGTTGGCAACCTGGTACCTCTACTTTTAGCCATTTCTCCTTCTCTATAAATAGTGCA
GAAGTAACCCACTTGGTAACAGGCATCCTTGCCAAGCCTCCACCACTAGGTCAGTGTAAGAATTAAAGAAAGAGGAAAGAAACACAAAAAGTGGCTTGAT
GGTTAAGACAGGTTTATTTTAGAGAAAACACACCTGAGAGGGGCTGCTGGCTGAATTAGGTTAGAGTCTTTTCTACAGACTAAGAGTGTTTAAGGATTTA
GGGTGGGAGAGTTTCTTAGAGGCTTGGACTGCTTCTGTGTTTTTTTTGTTGTGCTTATATGGGAGGGAGAGTGGTGTGTTTGCTTTTATACATTTTTCTG
CAGCTGTAGGCATACCCCCCAAGTCTGCTTTTAGCTTCCCTATTTTAGTGCACCTGGAGGGAAAGGAATGTGCTTATTAAGGCCCACTGTTTTACTGGGG
CCCATTGTATGAGGGTGAAGTTTGGCAGTTACCCAAGAGACTTTTCCTCCACCTTCCTCTGTGCCCGAGCTGTTTTATCTGCATTTTACTGTCTGCTTTT
TTTGGCTGCTTATAGTTTTTAAAAAAGTAATTTCCTTAAATCCAGAAGGCTAAAAATGAAGCTGAAACTTAAAGTGGCGGTGTTTGTCCAAAATAACGGG
GCTCCTGCTCTGCCAGTCAGTACCCTCAAGTCACTCCTGATCCTCAACCTCCATGCCTAAGGCTGGTTCAAGAGACCACATAATATCTGCCTTTTATTAC
ATACATGATGGGTGCATGGGATTCTGCGTGCCCTTTGCTTGATATAGACTGCTAAGGTGAGATGGGGAATATCAGAGTCAGCTGCTGCTTGAGGAAGCAG
AACACACAGCTGGAGGCTTGGAACATGTGGGTCCCTATGAGTGTAGAGCCCATATCCCCATAGAGTCTACCTAGAGCAGGGGTCGCCAAATGTTTTCTTA
AAGAGCCTGATAGTGTATATGTTAGGCTTTGTGAGCCAGGTATTTACAGCAACTCAATTCTACCACTGTGGTATGAAAACAGCTATAGACAATCATAAAT
GAATGATCATGGCTATGTTTTAATAAAACTTTACAGACACTGAACTTGAACTTCCATTGTGATATGAAAACAGCTATAGACAATCATAAATGAATGATCA
TGGCTATGTTTTAATAAAACTTTATGGACACTGAGCTTGAACTTCATATATCAGTCATGTGACACAAAATATCATTCTTCTTTTTATTTGTTTTCACCCA
TTTGAAAAATGTAAAAACTATTCTTAGCTGTACAGAAACAGATGGTGGGTCAGATTTGACTCACTGCCCATAGTTTCCAGACCATGATGTTCAGGTTCAT
GGAAGCACTTCACTCTACACTAATTTATTCATCCATTCATCACATATGTGATGAGCACATACAGACACACCTCAGAGATATTGCAGGCTCCACTCCAGAT
CACCACAATAATGTGAATATTGCAATAAAGCAAGTCATACACATT
- refseq_EMR2.F3 (forward primer)
- refseq_EMR2.R3 (reverse primer)
- Alternative acceptor
Frame 0
GGSALCGEASLLGTCLASEEGPFSLPGLLCGHC
NASVFFLSFCF*HRVSLYHPSWSAVAPS*LTAAS
NSPARVILPASAS*VVAAGTTDVCHQAQLHRQF
RGGPWASGCPPPLLYKVLASGQPAQLPSLRRRD
SPVPLTLSPAAPAGSSAGTMGGRVFLVFLAFCVW
LTLPGAETQDSRGCARWCPQDSSCVNATACRCN
PGFSSFSEIITTPMETCDDINECATLSKVSCGK
FSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESEN
TCQDMTFSTWTPPPGVHSQTLSRFFDKVQDLGR
DYKPGLANNTIQSILQALDELLEAPGDLETLPR
LQQHCVASHLLDGLEDVLRGLSKNLSNGLLNFSY
PAGTELSLEVQKQVDRSVTLRQNQAVMQLDWNQ
AQKSGDPGPSVVGLVSIPGMGKLLAEAPLVLEP
EKQMLLHETHQGLLQDGSPILLSDVISAFLSNND
TQNLSSPVTFTFSHRSVIPRQKVLCVFWEHGQN
GCGHWATTGCSTIGTRDTSTICRCTHLSSFAVL
MAHYDVQEEDPVLTVITYMGLSVSLLCLLLAALT
FLLCKAIQNTSTSLHLQLSLCLFLAHLLFLVAI
DQTGHKVLCSIIAGTLHYLYLATLTWMLLEALY
LFLTARNLTVVNYSSINRFMKKLMFPVGYGVPAV
TVAISAASRPHLYGTPSRCWLQPEKGFIWGFLG
PVCAIFSVNLVLFLVTLWILKNRLSSLNSEVST
LRNTRMLAFKATAQLFILGCTWCLGILQVGPAAR
VMAYLFTIINSLQGVFIFLVYCLLSQQVREQYG
KWSKGIRKLKTESEMHTLSSSAKADTSKPSTVN
*KNLLNKIFPLCPWKI*TIFEPSRGERKDFVLCV
SRNSPCQQYEGCYGRRARHSIPAETGNLPCPAM
CSSNSQHMDGQLWPISWSL*STIFMKL*NVKTS
FTASPSYKDSSKS*NEAGKRA*EDFHTDNI*KD*
NVHTTIWIS*FFVFVFVVL*FYGFDYLVM*KIL
ITHT*IKRDTAPAFMELLGENEVALAARVDSEA
EIPRNQVSTARQLKYKLFYKHCLLSSSHQHAEKF
LISDRGCATNSISKE*PAKLLNIPSKCP*PCLL
SAFLEQKFYMTIKPRRWVWHSSCPVILALWEGE
AGGWLKPRSLRPVWATE*EPSLPKKKIKN*PSMV
MI*ELRRNHLGK*QG*EVLSKVFHLMKSSPQNH
FLF*QRTACKIELQT*TSKLEASTGECRQLCQ*
EKAT*TRHVQNGGSKFPSLCQPCVQ*KAGNIVSA
KAHLHNKIRVGWPAHIGVGPR*IRHRLLKPVYK
IWYTMTRVRFPIQTPLSHARERAVLLSLSFAY*
TSAPGQAQWLTPIIPALWEAEVVRSPKVRSSRPA
WSTW*NLVSSKNTKIYEISHR**Y*VPKATQPG
RF*FFLRQGLALSPMLEYNGTNTAHCSLDLPRP
KQSSCLSPPPGI*NYRCVPPHRANFCIFCRDVGL
TMLPRLRS*SPELRQSAGLSLPKCGDYRLEPLH
LASICFTKEKLRS*MLSDLPEAIPLTGARVRIQ
TPSNWSQSWSFLHCPTLPTISILWAPFYKNQNIT
EHCFVNSAGNISQCNSHFSLLCACPYKLNEY*F
RGFGEL*MLPPK*PLLATWYLYF*PFLLLYK*C
RSNPLGNRHPCQASTTRSV*ELKKEERNTKSGLM
VKTGLF*RKHT*EGLLAELG*SLFYRLRVFKDL
GWESFLEAWTASVFFLLCLYGRESGVFAFIHFS
AAVGIPPKSAFSFPILVHLEGKECAY*GPLFYWG
PLYEGEVWQLPKRLFLHLPLCPSCFICILLSAF
FGCL*FLKK*FP*IQKAKNEAET*SGGVCPK*R
GSCSASQYPQVTPDPQPPCLRLVQETT*YLPFIT
YMMGAWDSACPLLDIDC*GEMGNIRVSCCLRKQ
NTQLEAWNMWVPMSVEPISP*SLPRAGVAKCFL
KEPDSVYVRLCEPGIYSNSILPLWYENSYRQS*M
NDHGYVLIKLYRH*T*TSIVI*KQL*TIINE*S
WLCFNKTLWTLSLNFIYQSCDTKYHSSFYLFSP
I*KM*KLFLAVQKQMVGQI*LTAHSFQTMMFRFM
EALHSTLIYSSIHHICDEHIQTHLRDIAGSTPD
HHNNVNIAIKQVIHI
Frame 1
GGLLCAVKLLFLAPAWHRKRAPSPSLGFYVDTV
MPQFSFFLFVFDTGSHSITQAGVQWHHLSSPQP
QTPQLG*FFLPQPPE**QLGPQTSATKPSCTASS
GEGPGPAAVRPPSFIKSWPRDSPHSCPACGDGT
ALSHSLFPLLLLPAAQLEPWEAASFSSFSHSVS
G*LCRELKPRTPGAVPGGALRTPRVSMPPPVAAI
QGSALFLRSSPPPWRLVTTSTSVQHCRKCHAEN
SRTAGTQRGATTACAAQDMSLFLGQKHSRMRAR
TRVKI*LSPPGPRPLESTARRFPDSSTKSRTWAE
TTSQAWPITPSRASYRRWMSCWRPLGTWRPCPA
YSSTVWPVTCWMA*RMSSEA*ARTFPMGC*TSV
ILQAQNCPWRCRSK*TGVSP*DRIRQ*CSSTGIR
HRNLVTQALLWWALSPFQGWASCWLRPLWSWNL
RSRCFCMRHTRACCRTAPPSCSQM*SLPF*ATT
TPKTSAPQLPSPSPTVQ*SRDRRCSVSSGSMARM
DVVTGPPQAAAQ*APETPAPSAVAPT*AALPSS
WPTTMCRRRIPC*LSSPTWG*ASLCCASSWRPS
LFSCVKPSRTPAPHCICSSRSASSWPTSSSSWQL
IKPDTRCCAPSSPVPCTISTWPP*PGCCWRPCT
SSSLHGT*RWSTTQASTDS*RSSCSLWATESQL
*QWPFLQPPGLTFMEHLPAAGSNQKRDLYGASLD
LSAPSSL*I*FSFW*LSGF*KTDSPPSIVKCPP
SGTQGCWHLKRQLSCSSWAARGVWASCRWVRLP
GSWPTSSPSSTACRVSSSSWCTASSASRSGSNMG
NGPKGSGN*KLSLRCTHSPAVLRLTPPNPARLT
RKIF*IRSSLFARGKSEQSLSHLEGKEKTLFCV
FQEIHHVSNMKDVMEGVLGIQFLQKPEIFHALQC
AHQTLSIWTASCGPYLGHSEAQYL*SYRTLRPL
SQPLLPTKTPPNLKMKQENEPKRTFIPTTSERT
RMFTPRSGFLNFLFLFLLFSSSTGLII*SCEKY*
LLTHRSRETRLLPSWSF*GKMKWLLQLELTQKP
KFLEIRFLLLGN*SINYFINTVFFRLHTNMQKS
F*SQIGDVQQIPFQRNDLQNS*IFQANALNPVCY
LLSLNRNST*P*NLEDGYGTVHAL*S*HFGRVR
QEDGSSPGV*DQCGQQSENHLYPKKKLKISQAW
**YRS*GEII*ANSKGRKSSVRFSI**KAAPKII
FFSNKEQPVKSSCRHRQASWKLPRVNAGSCANR
KKLPRLGMSKMAAPSSLLFASHVYSKKQAT*CQ
PKLICIIRLGWGGQLT*G*ALGKSDTAFSSLSIK
SGTL*RGSDFPFRRPSPMQEKELFSFLFLLPIK
PLLLARHSGSRL*SQHFGRLRWSDHLRSGVQDQ
PGQHGEILSLVKIQKYMKSHIDDNIKFQKQLNLV
DSNFF*GRVLLCHPCWSTMAQTLLTAASTSQGL
SNPPASVPLQVFETTGVYHHTGLIFVFFVETWV
SLCCPGSGLNLLSSGNPQASASLSAGITGLSHCT
*PLFVLQKRN*DPEC*VTCLRPSH*QEPGLGFK
PHPTGPRAGASCTALHYLPSPSSGHLFIRTKTL
QSIALSTQLGTFPSATHIFHCSVPVRISSMSIDL
GALENFECYPPSNHCWQPGTSTFSHFSFSINSA
EVTHLVTGILAKPPPLGQCKN*RKRKETQKVA*
WLRQVYFRENTPERGCWLN*VRVFSTD*ECLRI*
GGRVS*RLGLLLCFFCCAYMGGRVVCLLLYIFL
QL*AYPPSLLLASLF*CTWRERNVLIKAHCFTG
AHCMRVKFGSYPRDFSSTFLCARAVLSAFYCLLF
LAAYSF*KSNFLKSRRLKMKLKLKVAVFVQNNG
APALPVSTLKSLLILNLHA*GWFKRPHNICLLL
HT*WVHGILRALCLI*TAKVRWGISESAAA*GSR
THSWRLGTCGSL*V*SPYPHRVYLEQGSPNVFL
KSLIVYMLGFVSQVFTATQFYHCGMKTAIDNHK
*MIMAMF**NFTDTELELPL*YENSYRQS*MNDH
GYVLIKLYGH*A*TSYISHVTQNIILLFICFHP
FEKCKNYS*LYRNRWWVRFDSLPIVSRP*CSGS
WKHFTLH*FIHPFITYVMSTYRHTSEILQAPLQI
TTIM*ILQ*SKSYT
Frame 2
GVCSVR*SFSSWHLPGIGRGPLLPPWAFMWTL*
CLSFLSFFLFLTQGLTLSPKLECSGTILAHRSL
KLPSSGDSSCLSLLSSSSWDHRRLPPSPAAPPV
PGRALGQRLSAPPPL*SPGLGTARTAAQPAETGQ
PCPTHSFPCCSCRQLSWNHGRPRLSRLSRILCL
ADSAGS*NPGLQGLCPVVPSGLLVCQCHRLSLQ
SRVQLFF*DHHHPHGDL*RHQRVCNTVESVMRKI
LGLLEHRGELRLRVQPRI*ACFWGKNIQE*ERE
HVSRYDFLHLDPAPWSPQPDAFPILRQSPGPGQ
RLQARLGQ*HHPEHLTGAG*AAGGPWGPGDPAPL
TAALCGQSPAGWPRGCPQRPEQEPFQWAVELQL
SCRHRIVPGGAEASRQECHLETESGSDAARLES
GTEIW*PRPFCGGPCLHSRDGQVAG*GPSGPGT*
EADASA*DTPGLAAGRLPHPALRCDLCLSEQQR
HPKPQLPSYLHLLPPFSDPETEGALCLLGAWPE
WMWSLGHHRLQHNRHQRHQHHLPLHPPEQLCRPH
GPLRCAGGGSRADCHHLHGAERLSAVPPPGGPH
FSPV*SHPEHQHLTASAALALPLPGPPPLPRGN
*SNRTQGAVLHHRRYLALSLPGHLDLDAAGGPVP
LPHCTEPDGGQLLKHQQIHEEAHVPCGLRSPSC
DSGHFCSLQASPLWNTFPLLAPTRKGIYMGLPW
TCLRHLLCEFSSLSGDSLDFEKQTLLPQ**SVHP
PEHKDAGI*SDSSAVHPGLHVVSGHLAGGSGCP
GHGLPLHHHQQPAGCLHLPGVLPPQPAGPGAIW
EMVQRDQEIEN*V*DAHTLQQC*G*HLQTQHG*L
EKSSE*DLPSLPVENLNNL*AI*RGKKRLCSVC
FKKFTMSAI*RMLWKAC*AFNSCRNRKSSMPCN
VLIKLSAYGRPAVAHILVTLKHNIYEAIER*DLF
HSLSFLQRLLQILK*SRKTSLRGLSYRQHLKGL
ECSHHDLDFLIFCFCFCCSLVLRV*LFSHVKNI
DYSHIDQERHGSCLHGAFRGK*SGSCS*S*LRSR
NS*KSGFYC*AIEV*TIL*TLSSFVFTPTCRKV
SNLRSGMCNKFHFKGMTCKTPKYSKQMPLTLSV
ICFP*TEILHDHKTSKMGMAQFMPCNPSTLGG*G
RRMAQAQEFETSVGNRVRTISTQKKN*KLAKHG
DDIGVKEKSFRQIARVGSPQ*GFPFNEKQPPKS
FSFLTKNSL*NRAADIDKQAGSFHG*MPAAVPIG
KSYLD*ACPKWRLQVPFSLPAMCTVKSRQHSVS
QSSFA**D*GGVASSHRGRP*VNQTPPSQACL*
NLVHYDEGQISHSDAPLPCKRKSCSPFSFFCLLN
LCSWPGTVAHAYNPSTLGG*GGQIT*GQEFKTS
LVNMVKSCL**KYKNI*NLT*MIILSSKSNSTW
*ILIFFEAGSCFVTHAGVQWHKHCSLQPRPPKA*
AILLPQSPSRYLKLQVCTTTPG*FLYFL*RRGS
HYAAQAQVLIS*AQAIRRPQPP*VRGLQA*ATA
PSLYLFYKREIEILNVK*LA*GHPTNRSQG*DSN
PIQLVPELELLALPYTTYHLHPLGTFL*EPKHY
RALLCQLSWEHFPVQLTFFTALCLSV*AQ*VLI
*GLWRTLNATPQVTIVGNLVPLLLAISPSL*IVQ
K*PTW*QASLPSLHH*VSVRIKERGKKHKKWLD
G*DRFILEKTHLRGAAG*IRLESFLQTKSV*GF
RVGEFLRGLDCFCVFFVVLIWEGEWCVCFYTFFC
SCRHTPQVCF*LPYFSAPGGKGMCLLRPTVLLG
PIV*G*SLAVTQETFPPPSSVPELFYLHFTVCF
FWLLIVFKKVISLNPEG*K*S*NLKWRCLSKITG
LLLCQSVPSSHS*SSTSMPKAGSRDHIISAFYY
IHDGCMGFCVPFA*YRLLR*DGEYQSQLLLEEA
EHTAGGLEHVGPYECRAHIPIEST*SRGRQMFS*
RA**CIC*AL*ARYLQQLNSTTVV*KQL*TIIN
E*SWLCFNKTLQTLNLNFHCDMKTAIDNHK*MI
MAMF**NFMDTELELHISVM*HKISFFFLFVFTH
LKNVKTILSCTETDGGSDLTHCP*FPDHDVQVH
GSTSLYTNLFIHSSHM**AHTDTPQRYCRLHSR
SPQ*CEYCNKASHTH