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Sequences

Sequence NM_001001549

AAATGTAATTTGAAGAAGGCAGAAGGAACCCATGGCTTTAGCCGGCTGCCCAGATTCCTTTTTGCACCATCCGTACTACCAGGACAAGGTGGAGCAGACA CCTCGCAGTCAACAAGACCCGGCAGGACCAGGACTCCCCGCACAGTCTGACCGACTTGCGAATCACCAGGAGGATGATGTGGACCTGGAAGCCCTGGTGA ACGATATGAATGCATCCCTGGAGAGCCTGTACTCGGCCTGCAGCATGCAGTCAGACACGGTGCCCCTCCTGCAGAATGGCCAGCATGCCCGCAGCCAGCC TCGGGCTTCAGGCCCTCCTCGGTCCATCCAGCCACAGGTGTCCCCGAGGCAGAGGGTGCAGCGCTCCCAGCCTGTGCACATCCTCGCTGTCAGGCGCCTT CAGGAGGAAGACCAGCAGTTTAGAACCTCATCTCTGCCGGCCATCCCCAATCCTTTTCCTGAACTCTGTGGCCCTGGGAGCCCCCCTGTGCTCACGCCGG GTTCTTTACCTCCGAGCCAGGCCGCCGCAAAGCAGGATGTTAAAGTCTTTAGTGAAGATGGGACAAGCAAAGTGGTGGAGATTCTAGCAGACATGACAGC CAGAGACCTGTGCCAATTGCTGGTTTACAAAAGTCACTGTGTGGATGACAACAGCTGGACACTAGTGGAGCACCACCCGCACCTAGGATTAGAGAGGTGC TTGGAAGACCATGAGCTGGTGGTCCAGGTGGAGAGTACCATGGCCAGTGAGAGTAAATTTCTATTCAGGAAGAATTACGCAAAATACGAGTTCTTTAAAA ATCCCATGAATTTCTTCCCAGAACAGATGGTTACTTGGTGCCAGCAGTCAAATGGCAGTCAAACCCAGCTTTTGCAGGAACCCAGACACCTGCAGCTGCT GGCCGACCTGGAGGACAGCAACATCTTCTCCCTGATCGCTGGCAGGAAGCAGTACAACGCCCCTACAGACCACGGGCTCTGCATAAAGCCAAACAAAGTC AGGAATGAAACTAAAGAGCTGAGGTTGCTCTGTGCAGAGGACGAGCAAACCAGGACGTGCTGGATGACAGCGTTCAGACTCCTCAAGTATGGAATGCTCC TTTACCAGAATTACCGAATCCCTCAGCAGAGGAAGGCCTTGCTGTCCCCGTTCTCGACGCCAGTGCGCAGTGTCTCCGAGAACTCCCTCGTGGCAATGGA TTTTTCTGGGCAAACAGGACGCGTGATAGAGAATCCGGCAGAGGCCCAGAGCGCAGCCCTGGAGGAGGGCCACGCCTGGAGGAAGCGAAGCACACGGATG AACATCCTAGGTAGCCAAAGTCCCCTCCACCCTTCTACCCTAAGTACAGTGATTCACAGGACACAGCACTGGTTTCACGGGAGGATCTCCAGGGAGGAAT CCCACAGGATCATTAAACAGCAAGGGCTCGTGGATGGGCTTTTTCTCCTCCGTGACAGCCAGAGTAATCCAAAGGCATTTGTACTCACACTGTGTCATCA CCAGAAAATTAAAAATTTCCAGATCTTACCTTGCGAGGACGACGGGCAGACGTTCTTCAGCCTAGATGACGGGAACACCAAATTCTCTGACCTGATCCAG CTGGTTGACTTTTACCAGCTGAACAAAGGAGTCCTGCCTTGCAAACTCAAGCACCACTGCATCCGAGTGGCCTTATGACCGCAGATGTCCTCTCGGCTGA AGACTGGAGGAAGTGAACACTGGAGTGAAGAAGCGGTCTGTGCGTTGGTGAAGAACACACATCGATTCTGCACCTGGGGACCCAGAGCGAGATGGGTTTG TTCGGTGCCAGCCGACCAAGATTGACTAGTTTGTTGGACTTAAACGACGATTTGCTGCTGTGAACCCAGCAGGGTCGCCTCCCTCTGCATCGGCCAAATT GGGGAGGGCATGGAAGATCCAGCGGAAAGTTGAAAATAAACTGGAATGATCATCTTGGCTTGGGCCGCTTAGGAACAAGAACCGGAGAGAAGTGATTGGA AATGAACTCTTGCCCTGGAATAATCTTGACAATTAAAACTGATATGTTTACTTTTTTTGTATTGATCACTTTTTTGCACTCCTTCTTTGTTTTCAATATT GTATTCAGCCTATTGTAGGAGGGGGATGTGGCGTTTCAACTCATATAATACAGAAAGAGTTTTGAATGGGCAGATTTCAAACTGAATATGGGTCCCCAAA TGTTCCCAGAGGGTCCTCCACACCCTCTGCCGACTACCACGGTGTGGATTCAGCTCCCAAATGACAAACCCAGCCCTTCCCAGTATACTTGAAAAGCTTT CTTGTTAAAATAAAAGGTGTCACTGTGGTAGGCATTTGGCATATTTTGTGGACTCAGTCAAGCAACCACAGTCTGTTAATCATTTCTCTATGCTCAGATG TCAGATCCTCTTGTTATTAGTGTGTCTTGTTCTGCACAGTGCAGGAGACTTTATTCCTTTGGAAAATTCACTGTTCCACAAACAGCAGGCTGAATGGCCT CGCCTCTAGATTGACGTGGGCCAGCCTCCTTGAGACACACCTGGCACCCGTCATCGGCCAGCGGTGGATGCTGCATAATCCACCTGGGTACTTCAGCCTT GCGTTTCCACAGCCTTCAGCCTGTTCTAGAACGATCACTGCCTTACCCCTGCTGCTGCAGTGGTGTGAGTCGTTTCACGGCTGATGTCCCTCGGGGGATT AAAGGATCTAAAGAGAAAATGGCACCTGGTTGTCTTCGTGCTGTGTCTCATGGGTTTCCATAGTGATAAAGACAAGGAAACGCTGCAGGGGCCACAGGCA CAGGCTGATATTTAAAGATCTTTGCTTGCAGCCCTCCGTCCTGCTGAAAACCCCCATAAGCCAGTGAACACAGAGCAGCTAGAGGCTCCTCCTCTGCTGG CTTAGGGTCAGAAGTACCTCACAGTGGTTGTGGACATGGAAGAGTTTTGTCAACACAACACTTTGTCCCCGCTCCGGGAGATGAGTCAGATGGTGGCTTG AGTTGTCACTTGGTCCCCTCCGCCCCTCGGGTGGCCCCCTTTGCCACGTCCCCTTAGCTTAGTGATCAGGTGTGAGAGTGGCCATTTCCTTACCTTTGAT CCCTGTAAAGCAGAAAGGACTCCTTTGACAGGCGACAAACTACTGTGGTGAGCAGAATGATTTCCTTTTTCAAGACAACACCTGCCTGGCTTCTATTAAT GTGTGCTGGCCATGATATTGCCCCAAATCCGCCCCACTGAAGTGTTCCCTAAGGAACAGCATTTCTCTGCTCCTCAGTCAACCCCCGTAGCCTAGAGCAG TGTCACAAGCTTCAGTAAGGCCAGTCAGCTGGAAGTCAGTCTACCGTATAGTAACACTGTATGTCAGTCTACAGACCACACTCTAGTTGTTTTCCATGAA AGGTATACAAATGAAGAATTTTCTAGCAAAACATGTTTTTAACCATCAGTGCTCAATTGCATTTTCTTCCTTTCGCAGCCAGTCAGTCTTTCAAACTATT GACAGTAAGATAATTCTCACGTTCACACCTGGTGGCAGGCTTCACTGTAGGGACGGACATTGCAGTTACACCACGATTCCTTCCTCTTCACTGGCTCGAG GTAAACCCTTTTCAAGGAAAAACAACTCTAGGATTTCTTTTTTCTGTGTACGTAGACCAGTCCCATCAGTGTATAATCTCTCTCTCACACGCCTCTCTCC AATAGACAGCTTGTATTTGCAGTATTTCATATTTATAAATATGCGTTTATTTAAAAGGAGAACAAAAGCTTGACTCTGATTCACAGTTTTGTATGTAGCT GGTTTGACGTAGTCTTTTGTATTTTCCCTGCCGAAGTGAATTGTTGGAGAATGTAAACCGCCTCCACGTGGCGGCAGACTTCCTAAGGCCCCAGCTCGCT GGCCTCGCGCTGGGCGGCTGGGAATTCCACCTGAGAACAAGTCCCGCAAACCGGGGACGGAAGGACATTTGACTTTTATTTTTGTATTTAATTGACATGA ATGTAAAGGGGACAGCTCAGGGTTGTTTTGGAGCCTGTTGACTTTGTATCTCTGCCTGTGATTTTCTTTTCTAAATGAAACTCCATGTAGCAACCAGGAC GAAGTTGAGAAGGAAAACGCCAAATGCTTTGGTTATTAGAGTTTAATAGGTAAGCTCTGTTACACTAGGTGTTAGAGTTCCAGAATGTTCTTTTGTTTGC TAAACCTTGAAGAAACATGTGCCTCAGCCTAGATGTTTTGTCTTCTCTTTTCTGCACTTAATACCTGACAGTATGACCGATCTCTGCGCCTTTCTGGGGG CGGGCAAGCTGGCGGTAGATTTGTGATGTCACAGTGCAAACTGCAGTGACTGTAAATTGGCCTGGCGTGTATAAACGTTTTCAGGGAATGCAGAAGGTAT TAATGAAGAGACAAAACCTTTATTCCATGTGCTTTGCTTCATTCTGTACATAGCTCTTTGGCTCGTGAACCTAATTGTAAACTTTCAGGTATTTTTGTAC AAATAAGGGACTGATGTTCTGTTTCTTGTAATTAGAAATAAACATTAATACAGTGTTCTTCA

refseq_GRB10.F1 (forward primer)
refseq_GRB10.R1 (reverse primer)
Single exon skipping

Frame 0

KCNLKKAEGTHGFSRLPRFLFAPSVLPGQGGAD TSQSTRPGRTRTPRTV*PTCESPGG*CGPGSPGE RYECIPGEPVLGLQHAVRHGAPPAEWPACPQPA SGFRPSSVHPATGVPEAEGAALPACAHPRCQAP SGGRPAV*NLISAGHPQSFS*TLWPWEPPCAHAG FFTSEPGRRKAGC*SL**RWDKQSGGDSSRHDS QRPVPIAGLQKSLCG*QQLDTSGAPPAPRIREV LGRP*AGGPGGEYHGQ*E*ISIQEELRKIRVL*K SHEFLPRTDGYLVPAVKWQSNPAFAGTQTPAAA GRPGGQQHLLPDRWQEAVQRPYRPRALHKAKQS QE*N*RAEVALCRGRANQDVLDDSVQTPQVWNAP LPELPNPSAEEGLAVPVLDASAQCLRELPRGNG FFWANRTRDRESGRGPERSPGGGPRLEEAKHTD EHPR*PKSPPPFYPKYSDSQDTALVSREDLQGGI PQDH*TARARGWAFSPP*QPE*SKGICTHTVSS PEN*KFPDLTLRGRRADVLQPR*REHQIL*PDP AG*LLPAEQRSPALQTQAPLHPSGLMTADVLSAE DWRK*TLE*RSGLCVGEEHTSILHLGTQSEMGL FGASRPRLTSLLDLNDDLLL*TQQGRLPLHRPN WGGHGRSSGKLKINWNDHLGLGRLGTRTGEK*LE MNSCPGIILTIKTDMFTFFVLITFLHSFFVFNI VFSLL*EGDVAFQLI*YRKSFEWADFKLNMGPQ MFPEGPPHPLPTTTVWIQLPNDKPSPSQYT*KAF LLK*KVSLW*AFGIFCGLSQATTVC*SFLYAQM SDPLVISVSCSAQCRRLYSFGKFTVPQTAG*MA SPLD*RGPASLRHTWHPSSASGGCCIIHLGTSAL RFHSLQPVLERSLPYPCCCSGVSRFTADVPRGI KGSKEKMAPGCLRAVSHGFP***RQGNAAGATG TG*YLKIFACSPPSC*KPP*ASEHRAARGSSSAG LGSEVPHSGCGHGRVLSTQHFVPAPGDESDGGL SCHLVPSAPRVAPFATSP*LSDQV*EWPFPYL* SL*SRKDSFDRRQTTVVSRMISFFKTTPAWLLLM CAGHDIAPNPPH*SVP*GTAFLCSSVNPRSLEQ CHKLQ*GQSAGSQSTV**HCMSVYRPHSSCFP* KVYK*RIF*QNMFLTISAQLHFLPFAASQSFKLL TVR*FSRSHLVAGFTVGTDIAVTPRFLPLHWLE VNPFQGKTTLGFLFSVYVDQSHQCIISLSHASL Q*TACICSISYL*ICVYLKGEQKLDSDSQFCM*L V*RSLLYFPCRSELLENVNRLHVAADFLRPQLA GLALGGWEFHLRTSPANRGRKDI*LLFLYLIDM NVKGTAQGCFGAC*LCISACDFLF*MKLHVATRT KLRRKTPNALVIRV**VSSVTLGVRVPECSFVC *TLKKHVPQPRCFVFSFLHLIPDSMTDLCAFLG AGKLAVDL*CHSANCSDCKLAWRV*TFSGNAEGI NEETKPLFHVLCFILYIALWLVNLIVNFQVFLY K*GTDVLFLVIRNKH*YSVL

Frame 1

NVI*RRQKEPMALAGCPDSFLHHPYYQDKVEQT PRSQQDPAGPGLPAQSDRLANHQEDDVDLEALV NDMNASLESLYSACSMQSDTVPLLQNGQHARSQP RASGPPRSIQPQVSPRQRVQRSQPVHILAVRRL QEEDQQFRTSSLPAIPNPFPELCGPGSPPVLTP GSLPPSQAAAKQDVKVFSEDGTSKVVEILADMTA RDLCQLLVYKSHCVDDNSWTLVEHHPHLGLERC LEDHELVVQVESTMASESKFLFRKNYAKYEFFK NPMNFFPEQMVTWCQQSNGSQTQLLQEPRHLQLL ADLEDSNIFSLIAGRKQYNAPTDHGLCIKPNKV RNETKELRLLCAEDEQTRTCWMTAFRLLKYGML LYQNYRIPQQRKALLSPFSTPVRSVSENSLVAMD FSGQTGRVIENPAEAQSAALEEGHAWRKRSTRM NILGSQSPLHPSTLSTVIHRTQHWFHGRISREE SHRIIKQQGLVDGLFLLRDSQSNPKAFVLTLCHH QKIKNFQILPCEDDGQTFFSLDDGNTKFSDLIQ LVDFYQLNKGVLPCKLKHHCIRVAL*PQMSSRL KTGGSEHWSEEAVCALVKNTHRFCTWGPRARWVC SVPADQD*LVCWT*TTICCCEPSRVASLCIGQI GEGMEDPAES*K*TGMIILAWAA*EQEPERSDW K*TLALE*S*QLKLICLLFLY*SLFCTPSLFSIL YSAYCRRGMWRFNSYNTERVLNGQISN*IWVPK CSQRVLHTLCRLPRCGFSSQMTNPALPSILEKL SC*NKRCHCGRHLAYFVDSVKQPQSVNHFSMLRC QILLLLVCLVLHSAGDFIPLENSLFHKQQAEWP RL*IDVGQPP*DTPGTRHRPAVDAA*STWVLQP CVSTAFSLF*NDHCLTPAAAVV*VVSRLMSLGGL KDLKRKWHLVVFVLCLMGFHSDKDKETLQGPQA QADI*RSLLAALRPAENPHKPVNTEQLEAPPLL A*GQKYLTVVVDMEEFCQHNTLSPLREMSQMVA* VVTWSPPPLGWPPLPRPLSLVIRCESGHFLTFD PCKAERTPLTGDKLLW*AE*FPFSRQHLPGFY* CVLAMILPQIRPTEVFPKEQHFSAPQSTPVA*SS VTSFSKASQLEVSLPYSNTVCQSTDHTLVVFHE RYTNEEFSSKTCF*PSVLNCIFFLSQPVSLSNY *Q*DNSHVHTWWQASL*GRTLQLHHDSFLFTGSR *TLFKEKQL*DFFFLCT*TSPISV*SLSHTPLS NRQLVFAVFHIYKYAFI*KENKSLTLIHSFVCS WFDVVFCIFPAEVNCWRM*TASTWRQTS*GPSSL ASRWAAGNST*EQVPQTGDGRTFDFYFCI*LT* M*RGQLRVVLEPVDFVSLPVIFFSK*NSM*QPG RS*EGKRQMLWLLEFNR*ALLH*VLEFQNVLLFA KP*RNMCLSLDVLSSLFCT*YLTV*PISAPFWG RASWR*ICDVTVQTAVTVNWPGVYKRFQGMQKV LMKRQNLYSMCFASFCT*LFGS*T*L*TFRYFCT NKGLMFCFL*LEININTVFF

Frame 2

M*FEEGRRNPWL*PAAQIPFCTIRTTRTRWSRH LAVNKTRQDQDSPHSLTDLRITRRMMWTWKPW* TI*MHPWRACTRPAACSQTRCPSCRMASMPAAS LGLQALLGPSSHRCPRGRGCSAPSLCTSSLSGAF RRKTSSLEPHLCRPSPILFLNSVALGAPLCSRR VLYLRARPPQSRMLKSLVKMGQAKWWRF*QT*Q PETCANCWFTKVTVWMTTAGH*WSTTRT*D*RGA WKTMSWWSRWRVPWPVRVNFYSGRITQNTSSLK IP*ISSQNRWLLGASSQMAVKPSFCRNPDTCSC WPTWRTATSSP*SLAGSSTTPLQTTGSA*SQTKS GMKLKS*GCSVQRTSKPGRAG*QRSDSSSMECS FTRITESLSRGRPCCPRSRRQCAVSPRTPSWQW IFLGKQDA**RIRQRPRAQPWRRATPGGSEAHG* TS*VAKVPSTLLP*VQ*FTGHSTGFTGGSPGRN PTGSLNSKGSWMGFFSSVTARVIQRHLYSHCVI TRKLKISRSYLARTTGRRSSA*MTGTPNSLT*SS WLTFTS*TKESCLANSSTTASEWPYDRRCPLG* RLEEVNTGVKKRSVRW*RTHIDSAPGDPERDGF VRCQPTKID*FVGLKRRFAAVNPAGSPPSASAKL GRAWKIQRKVENKLE*SSWLGPLRNKNRREVIG NELLPWNNLDN*N*YVYFFCIDHFFALLLCFQY CIQPIVGGGCGVSTHIIQKEF*MGRFQTEYGSPN VPRGSSTPSADYHGVDSAPK*QTQPFPVYLKSF LVKIKGVTVVGIWHILWTQSSNHSLLIISLCSD VRSSCY*CVLFCTVQETLFLWKIHCSTNSRLNGL ASRLTWASLLETHLAPVIGQRWMLHNPPGYFSL AFPQPSACSRTITALPLLLQWCESFHG*CPSGD *RI*RENGTWLSSCCVSWVSIVIKTRKRCRGHRH RLIFKDLCLQPSVLLKTPISQ*TQSS*RLLLCW LRVRSTSQWLWTWKSFVNTTLCPRSGR*VRWWL ELSLGPLRPSGGPLCHVPLA**SGVRVAISLPLI PVKQKGLL*QATNYCGEQNDFLFQDNTCLASIN VCWP*YCPKSAPLKCSLRNSISLLLSQPP*PRA VSQASVRPVSWKSVYRIVTLYVSLQTTL*LFSMK GIQMKNFLAKHVFNHQCSIAFSSFRSQSVFQTI DSKIILTFTPGGRLHCRDGHCSYTTIPSSSLAR GKPFSRKNNSRISFFCVRRPVPSVYNLSLTRLSP IDSLYLQYFIFINMRLFKRRTKA*L*FTVLYVA GLT*SFVFSLPK*IVGECKPPPRGGRLPKAPAR WPRAGRLGIPPENKSRKPGTEGHLTFIFVFN*HE CKGDSSGLFWSLLTLYLCL*FSFLNETPCSNQD EVEKENAKCFGY*SLIGKLCYTRC*SSRMFFCL LNLEETCASA*MFCLLFSALNT*QYDRSLRLSGG GQAGGRFVMSQCKLQ*L*IGLACINVFRECRRY **RDKTFIPCALLHSVHSSLAREPNCKLSGIFV QIRD*CSVSCN*K*TLIQCSS

Sequence NM_001001555

GGGAGGTCGGGAGGAGGCAGAGAGGGAAGCGAGCTGCGGCCGGGCGGGCTCGGCGCTCGGAGACCCGGTGGAGCCCAAAGTTTCCGCGCAGCCCCTGGGT GGCGGCAGCGCCGGCGGCGCGGGGCGCCCGGGACAGTCTTGAGCGCCGGCCTCGCCCCGCGGGGACCCGCGCCCGCCGCCGGCCACGCCGAGTGTCGCCC GCAGCCACGCGGAGGCGGCGGGGAGCCGCGCGCGGCAGCTTTGGCGCTGACCACAATGCTGAGCAGGAAGCAGCAGCTGCAGGCCCAGTGACTGGTAGCT CAGTGACCAGCAGCCCAGTGACCGGCAGCCAGGTCCTCACCTGGGTCCTCTCAGTGAAGCCAGGGTGGCCGCCCCAGCAGACAGTGCTACAGAGCCAACT CCTGACAGGACAAGGTGGAGCAGACACCTCGCAGTCAACAAGACCCGGCAGGACCAGGACTCCCCGCACAGTCTGACCGACTTGCGAATCACCAGGAGGA TGATGTGGACCTGGAAGCCCTGGTGAACGATATGAATGCATCCCTGGAGAGCCTGTACTCGGCCTGCAGCATGCAGTCAGACACGGTGCCCCTCCTGCAG AATGGCCAGCATGCCCGCAGCCAGCCTCGGGCTTCAGGCCCTCCTCGGTCCATCCAGCCACAGGTGTCCCCGAGGCAGAGGGTGCAGCGCTCCCAGCCTG TGCACATCCTCGCTGTCAGGCGCCTTCAGGAGGAAGACCAGCAGTTTAGAACCTCATCTCTGCCGGCCATCCCCAATCCTTTTCCTGAACTCTGTGGCCC TGGGAGCCCCCCTGTGCTCACGCCGGGTTCTTTACCTCCGAGCCAGGCCGCCGCAAAGCAGGATGTTAAAGTCTTTAGTGAAGATGGGACAAGCAAAGTG GTGGAGATTCTAGCAGACATGACAGCCAGAGACCTGTGCCAATTGCTGGTTTACAAAAGTCACTGTGTGGATGACAACAGCTGGACACTAGTGGAGCACC ACCCGCACCTAGGATTAGAGAGGTGCTTGGAAGACCATGAGCTGGTGGTCCAGGTGGAGAGTACCATGGCCAGTGAGAGTAAATTTCTATTCAGGAAGAA TTACGCAAAATACGAGTTCTTTAAAAATCCCATGAATTTCTTCCCAGAACAGATGGTTACTTGGTGCCAGCAGTCAAATGGCAGTCAAACCCAGCTTTTG CAGAATTTTCTGAACTCCAGTAGTTGTCCTGAAATTCAAGGGTTTTTGCATGTGAAAGAGCTGGGAAAGAAATCATGGAAAAAGCTGTATGTGTGTTTGC GGAGATCTGGCCTTTATTGCTCCACCAAGGGAACTTCAAAGGAACCCAGACACCTGCAGCTGCTGGCCGACCTGGAGGACAGCAACATCTTCTCCCTGAT CGCTGGCAGGAAGCAGTACAACGCCCCTACAGACCACGGGCTCTGCATAAAGCCAAACAAAGTCAGGAATGAAACTAAAGAGCTGAGGTTGCTCTGTGCA GAGGACGAGCAAACCAGGACGTGCTGGATGACAGCGTTCAGACTCCTCAAGTATGGAATGCTCCTTTACCAGAATTACCGAATCCCTCAGCAGAGGAAGG CCTTGCTGTCCCCGTTCTCGACGCCAGTGCGCAGTGTCTCCGAGAACTCCCTCGTGGCAATGGATTTTTCTGGGCAAACAGGACGCGTGATAGAGAATCC GGCAGAGGCCCAGAGCGCAGCCCTGGAGGAGGGCCACGCCTGGAGGAAGCGAAGCACACGGATGAACATCCTAGGTAGCCAAAGTCCCCTCCACCCTTCT ACCCTAAGTACAGTGATTCACAGGACACAGCACTGGTTTCACGGGAGGATCTCCAGGGAGGAATCCCACAGGATCATTAAACAGCAAGGGCTCGTGGATG GGCTTTTTCTCCTCCGTGACAGCCAGAGTAATCCAAAGGCATTTGTACTCACACTGTGTCATCACCAGAAAATTAAAAATTTCCAGATCTTACCTTGCGA GGACGACGGGCAGACGTTCTTCAGCCTAGATGACGGGAACACCAAATTCTCTGACCTGATCCAGCTGGTTGACTTTTACCAGCTGAACAAAGGAGTCCTG CCTTGCAAACTCAAGCACCACTGCATCCGAGTGGCCTTATGACCGCAGATGTCCTCTCGGCTGAAGACTGGAGGAAGTGAACACTGGAGTGAAGAAGCGG TCTGTGCGTTGGTGAAGAACACACATCGATTCTGCACCTGGGGACCCAGAGCGAGATGGGTTTGTTCGGTGCCAGCCGACCAAGATTGACTAGTTTGTTG GACTTAAACGACGATTTGCTGCTGTGAACCCAGCAGGGTCGCCTCCCTCTGCATCGGCCAAATTGGGGAGGGCATGGAAGATCCAGCGGAAAGTTGAAAA TAAACTGGAATGATCATCTTGGCTTGGGCCGCTTAGGAACAAGAACCGGAGAGAAGTGATTGGAAATGAACTCTTGCCCTGGAATAATCTTGACAATTAA AACTGATATGTTTACTTTTTTTGTATTGATCACTTTTTTGCACTCCTTCTTTGTTTTCAATATTGTATTCAGCCTATTGTAGGAGGGGGATGTGGCGTTT CAACTCATATAATACAGAAAGAGTTTTGAATGGGCAGATTTCAAACTGAATATGGGTCCCCAAATGTTCCCAGAGGGTCCTCCACACCCTCTGCCGACTA CCACGGTGTGGATTCAGCTCCCAAATGACAAACCCAGCCCTTCCCAGTATACTTGAAAAGCTTTCTTGTTAAAATAAAAGGTGTCACTGTGGTAGGCATT TGGCATATTTTGTGGACTCAGTCAAGCAACCACAGTCTGTTAATCATTTCTCTATGCTCAGATGTCAGATCCTCTTGTTATTAGTGTGTCTTGTTCTGCA CAGTGCAGGAGACTTTATTCCTTTGGAAAATTCACTGTTCCACAAACAGCAGGCTGAATGGCCTCGCCTCTAGATTGACGTGGGCCAGCCTCCTTGAGAC ACACCTGGCACCCGTCATCGGCCAGCGGTGGATGCTGCATAATCCACCTGGGTACTTCAGCCTTGCGTTTCCACAGCCTTCAGCCTGTTCTAGAACGATC ACTGCCTTACCCCTGCTGCTGCAGTGGTGTGAGTCGTTTCACGGCTGATGTCCCTCGGGGGATTAAAGGATCTAAAGAGAAAATGGCACCTGGTTGTCTT CGTGCTGTGTCTCATGGGTTTCCATAGTGATAAAGACAAGGAAACGCTGCAGGGGCCACAGGCACAGGCTGATATTTAAAGATCTTTGCTTGCAGCCCTC CGTCCTGCTGAAAACCCCCATAAGCCAGTGAACACAGAGCAGCTAGAGGCTCCTCCTCTGCTGGCTTAGGGTCAGAAGTACCTCACAGTGGTTGTGGACA TGGAAGAGTTTTGTCAACACAACACTTTGTCCCCGCTCCGGGAGATGAGTCAGATGGTGGCTTGAGTTGTCACTTGGTCCCCTCCGCCCCTCGGGTGGCC CCCTTTGCCACGTCCCCTTAGCTTAGTGATCAGGTGTGAGAGTGGCCATTTCCTTACCTTTGATCCCTGTAAAGCAGAAAGGACTCCTTTGACAGGCGAC AAACTACTGTGGTGAGCAGAATGATTTCCTTTTTCAAGACAACACCTGCCTGGCTTCTATTAATGTGTGCTGGCCATGATATTGCCCCAAATCCGCCCCA CTGAAGTGTTCCCTAAGGAACAGCATTTCTCTGCTCCTCAGTCAACCCCCGTAGCCTAGAGCAGTGTCACAAGCTTCAGTAAGGCCAGTCAGCTGGAAGT CAGTCTACCGTATAGTAACACTGTATGTCAGTCTACAGACCACACTCTAGTTGTTTTCCATGAAAGGTATACAAATGAAGAATTTTCTAGCAAAACATGT TTTTAACCATCAGTGCTCAATTGCATTTTCTTCCTTTCGCAGCCAGTCAGTCTTTCAAACTATTGACAGTAAGATAATTCTCACGTTCACACCTGGTGGC AGGCTTCACTGTAGGGACGGACATTGCAGTTACACCACGATTCCTTCCTCTTCACTGGCTCGAGGTAAACCCTTTTCAAGGAAAAACAACTCTAGGATTT CTTTTTTCTGTGTACGTAGACCAGTCCCATCAGTGTATAATCTCTCTCTCACACGCCTCTCTCCAATAGACAGCTTGTATTTGCAGTATTTCATATTTAT AAATATGCGTTTATTTAAAAGGAGAACAAAAGCTTGACTCTGATTCACAGTTTTGTATGTAGCTGGTTTGACGTAGTCTTTTGTATTTTCCCTGCCGAAG TGAATTGTTGGAGAATGTAAACCGCCTCCACGTGGCGGCAGACTTCCTAAGGCCCCAGCTCGCTGGCCTCGCGCTGGGCGGCTGGGAATTCCACCTGAGA ACAAGTCCCGCAAACCGGGGACGGAAGGACATTTGACTTTTATTTTTGTATTTAATTGACATGAATGTAAAGGGGACAGCTCAGGGTTGTTTTGGAGCCT GTTGACTTTGTATCTCTGCCTGTGATTTTCTTTTCTAAATGAAACTCCATGTAGCAACCAGGACGAAGTTGAGAAGGAAAACGCCAAATGCTTTGGTTAT TAGAGTTTAATAGGTAAGCTCTGTTACACTAGGTGTTAGAGTTCCAGAATGTTCTTTTGTTTGCTAAACCTTGAAGAAACATGTGCCTCAGCCTAGATGT TTTGTCTTCTCTTTTCTGCACTTAATACCTGACAGTATGACCGATCTCTGCGCCTTTCTGGGGGCGGGCAAGCTGGCGGTAGATTTGTGATGTCACAGTG CAAACTGCAGTGACTGTAAATTGGCCTGGCGTGTATAAACGTTTTCAGGGAATGCAGAAGGTATTAATGAAGAGACAAAACCTTTATTCCATGTGCTTTG CTTCATTCTGTACATAGCTCTTTGGCTCGTGAACCTAATTGTAAACTTTCAGGTATTTTTGTACAAATAAGGGACTGATGTTCTGTTTCTTGTAATTAGA AATAAACATTAATACAGTGTTCTTCA

refseq_GRB10.F1 (forward primer)
refseq_GRB10.R1 (reverse primer)
Single exon skipping

Frame 0

GRSGGGREGSELRPGGLGARRPGGAQSFRAAPG WRQRRRRGAPGTVLSAGLAPRGPAPAAGHAECRP QPRGGGGEPRAAALALTTMLSRKQQLQAQ*LVA Q*PAAQ*PAARSSPGSSQ*SQGGRPSRQCYRAN S*QDKVEQTPRSQQDPAGPGLPAQSDRLANHQED DVDLEALVNDMNASLESLYSACSMQSDTVPLLQ NGQHARSQPRASGPPRSIQPQVSPRQRVQRSQP VHILAVRRLQEEDQQFRTSSLPAIPNPFPELCGP GSPPVLTPGSLPPSQAAAKQDVKVFSEDGTSKV VEILADMTARDLCQLLVYKSHCVDDNSWTLVEH HPHLGLERCLEDHELVVQVESTMASESKFLFRKN YAKYEFFKNPMNFFPEQMVTWCQQSNGSQTQLL QNFLNSSSCPEIQGFLHVKELGKKSWKKLYVCL RRSGLYCSTKGTSKEPRHLQLLADLEDSNIFSLI AGRKQYNAPTDHGLCIKPNKVRNETKELRLLCA EDEQTRTCWMTAFRLLKYGMLLYQNYRIPQQRK ALLSPFSTPVRSVSENSLVAMDFSGQTGRVIENP AEAQSAALEEGHAWRKRSTRMNILGSQSPLHPS TLSTVIHRTQHWFHGRISREESHRIIKQQGLVD GLFLLRDSQSNPKAFVLTLCHHQKIKNFQILPCE DDGQTFFSLDDGNTKFSDLIQLVDFYQLNKGVL PCKLKHHCIRVAL*PQMSSRLKTGGSEHWSEEA VCALVKNTHRFCTWGPRARWVCSVPADQD*LVCW T*TTICCCEPSRVASLCIGQIGEGMEDPAES*K *TGMIILAWAA*EQEPERSDWK*TLALE*S*QL KLICLLFLY*SLFCTPSLFSILYSAYCRRGMWRF NSYNTERVLNGQISN*IWVPKCSQRVLHTLCRL PRCGFSSQMTNPALPSILEKLSC*NKRCHCGRH LAYFVDSVKQPQSVNHFSMLRCQILLLLVCLVLH SAGDFIPLENSLFHKQQAEWPRL*IDVGQPP*D TPGTRHRPAVDAA*STWVLQPCVSTAFSLF*ND HCLTPAAAVV*VVSRLMSLGGLKDLKRKWHLVVF VLCLMGFHSDKDKETLQGPQAQADI*RSLLAAL RPAENPHKPVNTEQLEAPPLLA*GQKYLTVVVD MEEFCQHNTLSPLREMSQMVA*VVTWSPPPLGWP PLPRPLSLVIRCESGHFLTFDPCKAERTPLTGD KLLW*AE*FPFSRQHLPGFY*CVLAMILPQIRP TEVFPKEQHFSAPQSTPVA*SSVTSFSKASQLEV SLPYSNTVCQSTDHTLVVFHERYTNEEFSSKTC F*PSVLNCIFFLSQPVSLSNY*Q*DNSHVHTWW QASL*GRTLQLHHDSFLFTGSR*TLFKEKQL*DF FFLCT*TSPISV*SLSHTPLSNRQLVFAVFHIY KYAFI*KENKSLTLIHSFVCSWFDVVFCIFPAE VNCWRM*TASTWRQTS*GPSSLASRWAAGNST*E QVPQTGDGRTFDFYFCI*LT*M*RGQLRVVLEP VDFVSLPVIFFSK*NSM*QPGRS*EGKRQMLWL LEFNR*ALLH*VLEFQNVLLFAKP*RNMCLSLDV LSSLFCT*YLTV*PISAPFWGRASWR*ICDVTV QTAVTVNWPGVYKRFQGMQKVLMKRQNLYSMCF ASFCT*LFGS*T*L*TFRYFCTNKGLMFCFL*LE ININTVFF

Frame 1

GGREEAEREASCGRAGSALGDPVEPKVSAQPLG GGSAGGAGRPGQS*APASPRGDPRPPPATPSVA RSHAEAAGSRARQLWR*PQC*AGSSSCRPSDW*L SDQQPSDRQPGPHLGPLSEARVAAPADSATEPT PDRTRWSRHLAVNKTRQDQDSPHSLTDLRITRR MMWTWKPW*TI*MHPWRACTRPAACSQTRCPSCR MASMPAASLGLQALLGPSSHRCPRGRGCSAPSL CTSSLSGAFRRKTSSLEPHLCRPSPILFLNSVA LGAPLCSRRVLYLRARPPQSRMLKSLVKMGQAKW WRF*QT*QPETCANCWFTKVTVWMTTAGH*WST TRT*D*RGAWKTMSWWSRWRVPWPVRVNFYSGR ITQNTSSLKIP*ISSQNRWLLGASSQMAVKPSFC RIF*TPVVVLKFKGFCM*KSWERNHGKSCMCVC GDLAFIAPPRELQRNPDTCSCWPTWRTATSSP* SLAGSSTTPLQTTGSA*SQTKSGMKLKS*GCSVQ RTSKPGRAG*QRSDSSSMECSFTRITESLSRGR PCCPRSRRQCAVSPRTPSWQWIFLGKQDA**RI RQRPRAQPWRRATPGGSEAHG*TS*VAKVPSTLL P*VQ*FTGHSTGFTGGSPGRNPTGSLNSKGSWM GFFSSVTARVIQRHLYSHCVITRKLKISRSYLA RTTGRRSSA*MTGTPNSLT*SSWLTFTS*TKESC LANSSTTASEWPYDRRCPLG*RLEEVNTGVKKR SVRW*RTHIDSAPGDPERDGFVRCQPTKID*FV GLKRRFAAVNPAGSPPSASAKLGRAWKIQRKVEN KLE*SSWLGPLRNKNRREVIGNELLPWNNLDN* N*YVYFFCIDHFFALLLCFQYCIQPIVGGGCGV STHIIQKEF*MGRFQTEYGSPNVPRGSSTPSADY HGVDSAPK*QTQPFPVYLKSFLVKIKGVTVVGI WHILWTQSSNHSLLIISLCSDVRSSCY*CVLFC TVQETLFLWKIHCSTNSRLNGLASRLTWASLLET HLAPVIGQRWMLHNPPGYFSLAFPQPSACSRTI TALPLLLQWCESFHG*CPSGD*RI*RENGTWLS SCCVSWVSIVIKTRKRCRGHRHRLIFKDLCLQPS VLLKTPISQ*TQSS*RLLLCWLRVRSTSQWLWT WKSFVNTTLCPRSGR*VRWWLELSLGPLRPSGG PLCHVPLA**SGVRVAISLPLIPVKQKGLL*QAT NYCGEQNDFLFQDNTCLASINVCWP*YCPKSAP LKCSLRNSISLLLSQPP*PRAVSQASVRPVSWK SVYRIVTLYVSLQTTL*LFSMKGIQMKNFLAKHV FNHQCSIAFSSFRSQSVFQTIDSKIILTFTPGG RLHCRDGHCSYTTIPSSSLARGKPFSRKNNSRI SFFCVRRPVPSVYNLSLTRLSPIDSLYLQYFIFI NMRLFKRRTKA*L*FTVLYVAGLT*SFVFSLPK *IVGECKPPPRGGRLPKAPARWPRAGRLGIPPE NKSRKPGTEGHLTFIFVFN*HECKGDSSGLFWSL LTLYLCL*FSFLNETPCSNQDEVEKENAKCFGY *SLIGKLCYTRC*SSRMFFCLLNLEETCASA*M FCLLFSALNT*QYDRSLRLSGGGQAGGRFVMSQC KLQ*L*IGLACINVFRECRRY**RDKTFIPCAL LHSVHSSLAREPNCKLSGIFVQIRD*CSVSCN* K*TLIQCSS

Frame 2

EVGRRQRGKRAAAGRARRSETRWSPKFPRSPWV AAAPAARGARDSLERRPRPAGTRARRRPRRVSP AATRRRRGAARGSFGADHNAEQEAAAAGPVTGS SVTSSPVTGSQVLTWVLSVKPGWPPQQTVLQSQL LTGQGGADTSQSTRPGRTRTPRTV*PTCESPGG *CGPGSPGERYECIPGEPVLGLQHAVRHGAPPA EWPACPQPASGFRPSSVHPATGVPEAEGAALPAC AHPRCQAPSGGRPAV*NLISAGHPQSFS*TLWP WEPPCAHAGFFTSEPGRRKAGC*SL**RWDKQS GGDSSRHDSQRPVPIAGLQKSLCG*QQLDTSGAP PAPRIREVLGRP*AGGPGGEYHGQ*E*ISIQEE LRKIRVL*KSHEFLPRTDGYLVPAVKWQSNPAF AEFSELQ*LS*NSRVFACERAGKEIMEKAVCVFA EIWPLLLHQGNFKGTQTPAAAGRPGGQQHLLPD RWQEAVQRPYRPRALHKAKQSQE*N*RAEVALC RGRANQDVLDDSVQTPQVWNAPLPELPNPSAEEG LAVPVLDASAQCLRELPRGNGFFWANRTRDRES GRGPERSPGGGPRLEEAKHTDEHPR*PKSPPPF YPKYSDSQDTALVSREDLQGGIPQDH*TARARGW AFSPP*QPE*SKGICTHTVSSPEN*KFPDLTLR GRRADVLQPR*REHQIL*PDPAG*LLPAEQRSP ALQTQAPLHPSGLMTADVLSAEDWRK*TLE*RSG LCVGEEHTSILHLGTQSEMGLFGASRPRLTSLL DLNDDLLL*TQQGRLPLHRPNWGGHGRSSGKLK INWNDHLGLGRLGTRTGEK*LEMNSCPGIILTIK TDMFTFFVLITFLHSFFVFNIVFSLL*EGDVAF QLI*YRKSFEWADFKLNMGPQMFPEGPPHPLPT TTVWIQLPNDKPSPSQYT*KAFLLK*KVSLW*AF GIFCGLSQATTVC*SFLYAQMSDPLVISVSCSA QCRRLYSFGKFTVPQTAG*MASPLD*RGPASLR HTWHPSSASGGCCIIHLGTSALRFHSLQPVLERS LPYPCCCSGVSRFTADVPRGIKGSKEKMAPGCL RAVSHGFP***RQGNAAGATGTG*YLKIFACSP PSC*KPP*ASEHRAARGSSSAGLGSEVPHSGCGH GRVLSTQHFVPAPGDESDGGLSCHLVPSAPRVA PFATSP*LSDQV*EWPFPYL*SL*SRKDSFDRR QTTVVSRMISFFKTTPAWLLLMCAGHDIAPNPPH *SVP*GTAFLCSSVNPRSLEQCHKLQ*GQSAGS QSTV**HCMSVYRPHSSCFP*KVYK*RIF*QNM FLTISAQLHFLPFAASQSFKLLTVR*FSRSHLVA GFTVGTDIAVTPRFLPLHWLEVNPFQGKTTLGF LFSVYVDQSHQCIISLSHASLQ*TACICSISYL *ICVYLKGEQKLDSDSQFCM*LV*RSLLYFPCRS ELLENVNRLHVAADFLRPQLAGLALGGWEFHLR TSPANRGRKDI*LLFLYLIDMNVKGTAQGCFGA C*LCISACDFLF*MKLHVATRTKLRRKTPNALVI RV**VSSVTLGVRVPECSFVC*TLKKHVPQPRC FVFSFLHLIPDSMTDLCAFLGAGKLAVDL*CHS ANCSDCKLAWRV*TFSGNAEGINEETKPLFHVLC FILYIALWLVNLIVNFQVFLYK*GTDVLFLVIR NKH*YSVL

Sequence NM_001001550

CGCAACTTTGCCTCCCAGGGAACAAACATCCTCCTTCTAAGTGGTAGATGTGGGTGAGCTGACCCTGCTGGAGTCTGTCCCCTGGGCTACCCTCTGCTTC CCCCCATTGTGAGTGGTCCGTGAAGCACAGCGTTGACCAGACCTAAACCTGTTTGCTCCCAGGACAAGGTGGAGCAGACACCTCGCAGTCAACAAGACCC GGCAGGACCAGGACTCCCCGCACAGTCTGACCGACTTGCGAATCACCAGGAGGATGATGTGGACCTGGAAGCCCTGGTGAACGATATGAATGCATCCCTG GAGAGCCTGTACTCGGCCTGCAGCATGCAGTCAGACACGGTGCCCCTCCTGCAGAATGGCCAGCATGCCCGCAGCCAGCCTCGGGCTTCAGGCCCTCCTC GGTCCATCCAGCCACAGGTGTCCCCGAGGCAGAGGGTGCAGCGCTCCCAGCCTGTGCACATCCTCGCTGTCAGGCGCCTTCAGGAGGAAGACCAGCAGTT TAGAACCTCATCTCTGCCGGCCATCCCCAATCCTTTTCCTGAACTCTGTGGCCCTGGGAGCCCCCCTGTGCTCACGCCGGGTTCTTTACCTCCGAGCCAG GCCGCCGCAAAGCAGGATGTTAAAGTCTTTAGTGAAGATGGGACAAGCAAAGTGGTGGAGATTCTAGCAGACATGACAGCCAGAGACCTGTGCCAATTGC TGGTTTACAAAAGTCACTGTGTGGATGACAACAGCTGGACACTAGTGGAGCACCACCCGCACCTAGGATTAGAGAGGTGCTTGGAAGACCATGAGCTGGT GGTCCAGGTGGAGAGTACCATGGCCAGTGAGAGTAAATTTCTATTCAGGAAGAATTACGCAAAATACGAGTTCTTTAAAAATCCCATGAATTTCTTCCCA GAACAGATGGTTACTTGGTGCCAGCAGTCAAATGGCAGTCAAACCCAGCTTTTGCAGAATTTTCTGAACTCCAGTAGTTGTCCTGAAATTCAAGGGTTTT TGCATGTGAAAGAGCTGGGAAAGAAATCATGGAAAAAGCTGTATGTGTGTTTGCGGAGATCTGGCCTTTATTGCTCCACCAAGGGAACTTCAAAGGAACC CAGACACCTGCAGCTGCTGGCCGACCTGGAGGACAGCAACATCTTCTCCCTGATCGCTGGCAGGAAGCAGTACAACGCCCCTACAGACCACGGGCTCTGC ATAAAGCCAAACAAAGTCAGGAATGAAACTAAAGAGCTGAGGTTGCTCTGTGCAGAGGACGAGCAAACCAGGACGTGCTGGATGACAGCGTTCAGACTCC TCAAGTATGGAATGCTCCTTTACCAGAATTACCGAATCCCTCAGCAGAGGAAGGCCTTGCTGTCCCCGTTCTCGACGCCAGTGCGCAGTGTCTCCGAGAA CTCCCTCGTGGCAATGGATTTTTCTGGGCAAACAGGACGCGTGATAGAGAATCCGGCAGAGGCCCAGAGCGCAGCCCTGGAGGAGGGCCACGCCTGGAGG AAGCGAAGCACACGGATGAACATCCTAGGTAGCCAAAGTCCCCTCCACCCTTCTACCCTAAGTACAGTGATTCACAGGACACAGCACTGGTTTCACGGGA GGATCTCCAGGGAGGAATCCCACAGGATCATTAAACAGCAAGGGCTCGTGGATGGGCTTTTTCTCCTCCGTGACAGCCAGAGTAATCCAAAGGCATTTGT ACTCACACTGTGTCATCACCAGAAAATTAAAAATTTCCAGATCTTACCTTGCGAGGACGACGGGCAGACGTTCTTCAGCCTAGATGACGGGAACACCAAA TTCTCTGACCTGATCCAGCTGGTTGACTTTTACCAGCTGAACAAAGGAGTCCTGCCTTGCAAACTCAAGCACCACTGCATCCGAGTGGCCTTATGACCGC AGATGTCCTCTCGGCTGAAGACTGGAGGAAGTGAACACTGGAGTGAAGAAGCGGTCTGTGCGTTGGTGAAGAACACACATCGATTCTGCACCTGGGGACC CAGAGCGAGATGGGTTTGTTCGGTGCCAGCCGACCAAGATTGACTAGTTTGTTGGACTTAAACGACGATTTGCTGCTGTGAACCCAGCAGGGTCGCCTCC CTCTGCATCGGCCAAATTGGGGAGGGCATGGAAGATCCAGCGGAAAGTTGAAAATAAACTGGAATGATCATCTTGGCTTGGGCCGCTTAGGAACAAGAAC CGGAGAGAAGTGATTGGAAATGAACTCTTGCCCTGGAATAATCTTGACAATTAAAACTGATATGTTTACTTTTTTTGTATTGATCACTTTTTTGCACTCC TTCTTTGTTTTCAATATTGTATTCAGCCTATTGTAGGAGGGGGATGTGGCGTTTCAACTCATATAATACAGAAAGAGTTTTGAATGGGCAGATTTCAAAC TGAATATGGGTCCCCAAATGTTCCCAGAGGGTCCTCCACACCCTCTGCCGACTACCACGGTGTGGATTCAGCTCCCAAATGACAAACCCAGCCCTTCCCA GTATACTTGAAAAGCTTTCTTGTTAAAATAAAAGGTGTCACTGTGGTAGGCATTTGGCATATTTTGTGGACTCAGTCAAGCAACCACAGTCTGTTAATCA TTTCTCTATGCTCAGATGTCAGATCCTCTTGTTATTAGTGTGTCTTGTTCTGCACAGTGCAGGAGACTTTATTCCTTTGGAAAATTCACTGTTCCACAAA CAGCAGGCTGAATGGCCTCGCCTCTAGATTGACGTGGGCCAGCCTCCTTGAGACACACCTGGCACCCGTCATCGGCCAGCGGTGGATGCTGCATAATCCA CCTGGGTACTTCAGCCTTGCGTTTCCACAGCCTTCAGCCTGTTCTAGAACGATCACTGCCTTACCCCTGCTGCTGCAGTGGTGTGAGTCGTTTCACGGCT GATGTCCCTCGGGGGATTAAAGGATCTAAAGAGAAAATGGCACCTGGTTGTCTTCGTGCTGTGTCTCATGGGTTTCCATAGTGATAAAGACAAGGAAACG CTGCAGGGGCCACAGGCACAGGCTGATATTTAAAGATCTTTGCTTGCAGCCCTCCGTCCTGCTGAAAACCCCCATAAGCCAGTGAACACAGAGCAGCTAG AGGCTCCTCCTCTGCTGGCTTAGGGTCAGAAGTACCTCACAGTGGTTGTGGACATGGAAGAGTTTTGTCAACACAACACTTTGTCCCCGCTCCGGGAGAT GAGTCAGATGGTGGCTTGAGTTGTCACTTGGTCCCCTCCGCCCCTCGGGTGGCCCCCTTTGCCACGTCCCCTTAGCTTAGTGATCAGGTGTGAGAGTGGC CATTTCCTTACCTTTGATCCCTGTAAAGCAGAAAGGACTCCTTTGACAGGCGACAAACTACTGTGGTGAGCAGAATGATTTCCTTTTTCAAGACAACACC TGCCTGGCTTCTATTAATGTGTGCTGGCCATGATATTGCCCCAAATCCGCCCCACTGAAGTGTTCCCTAAGGAACAGCATTTCTCTGCTCCTCAGTCAAC CCCCGTAGCCTAGAGCAGTGTCACAAGCTTCAGTAAGGCCAGTCAGCTGGAAGTCAGTCTACCGTATAGTAACACTGTATGTCAGTCTACAGACCACACT CTAGTTGTTTTCCATGAAAGGTATACAAATGAAGAATTTTCTAGCAAAACATGTTTTTAACCATCAGTGCTCAATTGCATTTTCTTCCTTTCGCAGCCAG TCAGTCTTTCAAACTATTGACAGTAAGATAATTCTCACGTTCACACCTGGTGGCAGGCTTCACTGTAGGGACGGACATTGCAGTTACACCACGATTCCTT CCTCTTCACTGGCTCGAGGTAAACCCTTTTCAAGGAAAAACAACTCTAGGATTTCTTTTTTCTGTGTACGTAGACCAGTCCCATCAGTGTATAATCTCTC TCTCACACGCCTCTCTCCAATAGACAGCTTGTATTTGCAGTATTTCATATTTATAAATATGCGTTTATTTAAAAGGAGAACAAAAGCTTGACTCTGATTC ACAGTTTTGTATGTAGCTGGTTTGACGTAGTCTTTTGTATTTTCCCTGCCGAAGTGAATTGTTGGAGAATGTAAACCGCCTCCACGTGGCGGCAGACTTC CTAAGGCCCCAGCTCGCTGGCCTCGCGCTGGGCGGCTGGGAATTCCACCTGAGAACAAGTCCCGCAAACCGGGGACGGAAGGACATTTGACTTTTATTTT TGTATTTAATTGACATGAATGTAAAGGGGACAGCTCAGGGTTGTTTTGGAGCCTGTTGACTTTGTATCTCTGCCTGTGATTTTCTTTTCTAAATGAAACT CCATGTAGCAACCAGGACGAAGTTGAGAAGGAAAACGCCAAATGCTTTGGTTATTAGAGTTTAATAGGTAAGCTCTGTTACACTAGGTGTTAGAGTTCCA GAATGTTCTTTTGTTTGCTAAACCTTGAAGAAACATGTGCCTCAGCCTAGATGTTTTGTCTTCTCTTTTCTGCACTTAATACCTGACAGTATGACCGATC TCTGCGCCTTTCTGGGGGCGGGCAAGCTGGCGGTAGATTTGTGATGTCACAGTGCAAACTGCAGTGACTGTAAATTGGCCTGGCGTGTATAAACGTTTTC AGGGAATGCAGAAGGTATTAATGAAGAGACAAAACCTTTATTCCATGTGCTTTGCTTCATTCTGTACATAGCTCTTTGGCTCGTGAACCTAATTGTAAAC TTTCAGGTATTTTTGTACAAATAAGGGACTGATGTTCTGTTTCTTGTAATTAGAAATAAACATTAATACAGTGTTCTTCA

refseq_GRB10.F1 (forward primer)
refseq_GRB10.R1 (reverse primer)
Single exon skipping

Frame 0

RNFASQGTNILLLSGRCG*ADPAGVCPLGYPLL PPIVSGP*STALTRPKPVCSQDKVEQTPRSQQDP AGPGLPAQSDRLANHQEDDVDLEALVNDMNASL ESLYSACSMQSDTVPLLQNGQHARSQPRASGPP RSIQPQVSPRQRVQRSQPVHILAVRRLQEEDQQF RTSSLPAIPNPFPELCGPGSPPVLTPGSLPPSQ AAAKQDVKVFSEDGTSKVVEILADMTARDLCQL LVYKSHCVDDNSWTLVEHHPHLGLERCLEDHELV VQVESTMASESKFLFRKNYAKYEFFKNPMNFFP EQMVTWCQQSNGSQTQLLQNFLNSSSCPEIQGF LHVKELGKKSWKKLYVCLRRSGLYCSTKGTSKEP RHLQLLADLEDSNIFSLIAGRKQYNAPTDHGLC IKPNKVRNETKELRLLCAEDEQTRTCWMTAFRL LKYGMLLYQNYRIPQQRKALLSPFSTPVRSVSEN SLVAMDFSGQTGRVIENPAEAQSAALEEGHAWR KRSTRMNILGSQSPLHPSTLSTVIHRTQHWFHG RISREESHRIIKQQGLVDGLFLLRDSQSNPKAFV LTLCHHQKIKNFQILPCEDDGQTFFSLDDGNTK FSDLIQLVDFYQLNKGVLPCKLKHHCIRVAL*P QMSSRLKTGGSEHWSEEAVCALVKNTHRFCTWGP RARWVCSVPADQD*LVCWT*TTICCCEPSRVAS LCIGQIGEGMEDPAES*K*TGMIILAWAA*EQE PERSDWK*TLALE*S*QLKLICLLFLY*SLFCTP SLFSILYSAYCRRGMWRFNSYNTERVLNGQISN *IWVPKCSQRVLHTLCRLPRCGFSSQMTNPALP SILEKLSC*NKRCHCGRHLAYFVDSVKQPQSVNH FSMLRCQILLLLVCLVLHSAGDFIPLENSLFHK QQAEWPRL*IDVGQPP*DTPGTRHRPAVDAA*S TWVLQPCVSTAFSLF*NDHCLTPAAAVV*VVSRL MSLGGLKDLKRKWHLVVFVLCLMGFHSDKDKET LQGPQAQADI*RSLLAALRPAENPHKPVNTEQL EAPPLLA*GQKYLTVVVDMEEFCQHNTLSPLREM SQMVA*VVTWSPPPLGWPPLPRPLSLVIRCESG HFLTFDPCKAERTPLTGDKLLW*AE*FPFSRQH LPGFY*CVLAMILPQIRPTEVFPKEQHFSAPQST PVA*SSVTSFSKASQLEVSLPYSNTVCQSTDHT LVVFHERYTNEEFSSKTCF*PSVLNCIFFLSQP VSLSNY*Q*DNSHVHTWWQASL*GRTLQLHHDSF LFTGSR*TLFKEKQL*DFFFLCT*TSPISV*SL SHTPLSNRQLVFAVFHIYKYAFI*KENKSLTLI HSFVCSWFDVVFCIFPAEVNCWRM*TASTWRQTS *GPSSLASRWAAGNST*EQVPQTGDGRTFDFYF CI*LT*M*RGQLRVVLEPVDFVSLPVIFFSK*N SM*QPGRS*EGKRQMLWLLEFNR*ALLH*VLEFQ NVLLFAKP*RNMCLSLDVLSSLFCT*YLTV*PI SAPFWGRASWR*ICDVTVQTAVTVNWPGVYKRF QGMQKVLMKRQNLYSMCFASFCT*LFGS*T*L*T FRYFCTNKGLMFCFL*LEININTVFF

Frame 1

ATLPPREQTSSF*VVDVGELTLLESVPWATLCF PPL*VVREAQR*PDLNLFAPRTRWSRHLAVNKT RQDQDSPHSLTDLRITRRMMWTWKPW*TI*MHPW RACTRPAACSQTRCPSCRMASMPAASLGLQALL GPSSHRCPRGRGCSAPSLCTSSLSGAFRRKTSS LEPHLCRPSPILFLNSVALGAPLCSRRVLYLRAR PPQSRMLKSLVKMGQAKWWRF*QT*QPETCANC WFTKVTVWMTTAGH*WSTTRT*D*RGAWKTMSW WSRWRVPWPVRVNFYSGRITQNTSSLKIP*ISSQ NRWLLGASSQMAVKPSFCRIF*TPVVVLKFKGF CM*KSWERNHGKSCMCVCGDLAFIAPPRELQRN PDTCSCWPTWRTATSSP*SLAGSSTTPLQTTGSA *SQTKSGMKLKS*GCSVQRTSKPGRAG*QRSDS SSMECSFTRITESLSRGRPCCPRSRRQCAVSPR TPSWQWIFLGKQDA**RIRQRPRAQPWRRATPGG SEAHG*TS*VAKVPSTLLP*VQ*FTGHSTGFTG GSPGRNPTGSLNSKGSWMGFFSSVTARVIQRHL YSHCVITRKLKISRSYLARTTGRRSSA*MTGTPN SLT*SSWLTFTS*TKESCLANSSTTASEWPYDR RCPLG*RLEEVNTGVKKRSVRW*RTHIDSAPGD PERDGFVRCQPTKID*FVGLKRRFAAVNPAGSPP SASAKLGRAWKIQRKVENKLE*SSWLGPLRNKN RREVIGNELLPWNNLDN*N*YVYFFCIDHFFAL LLCFQYCIQPIVGGGCGVSTHIIQKEF*MGRFQT EYGSPNVPRGSSTPSADYHGVDSAPK*QTQPFP VYLKSFLVKIKGVTVVGIWHILWTQSSNHSLLI ISLCSDVRSSCY*CVLFCTVQETLFLWKIHCSTN SRLNGLASRLTWASLLETHLAPVIGQRWMLHNP PGYFSLAFPQPSACSRTITALPLLLQWCESFHG *CPSGD*RI*RENGTWLSSCCVSWVSIVIKTRKR CRGHRHRLIFKDLCLQPSVLLKTPISQ*TQSS* RLLLCWLRVRSTSQWLWTWKSFVNTTLCPRSGR *VRWWLELSLGPLRPSGGPLCHVPLA**SGVRVA ISLPLIPVKQKGLL*QATNYCGEQNDFLFQDNT CLASINVCWP*YCPKSAPLKCSLRNSISLLLSQ PP*PRAVSQASVRPVSWKSVYRIVTLYVSLQTTL *LFSMKGIQMKNFLAKHVFNHQCSIAFSSFRSQ SVFQTIDSKIILTFTPGGRLHCRDGHCSYTTIP SSSLARGKPFSRKNNSRISFFCVRRPVPSVYNLS LTRLSPIDSLYLQYFIFINMRLFKRRTKA*L*F TVLYVAGLT*SFVFSLPK*IVGECKPPPRGGRL PKAPARWPRAGRLGIPPENKSRKPGTEGHLTFIF VFN*HECKGDSSGLFWSLLTLYLCL*FSFLNET PCSNQDEVEKENAKCFGY*SLIGKLCYTRC*SS RMFFCLLNLEETCASA*MFCLLFSALNT*QYDRS LRLSGGGQAGGRFVMSQCKLQ*L*IGLACINVF RECRRY**RDKTFIPCALLHSVHSSLAREPNCK LSGIFVQIRD*CSVSCN*K*TLIQCSS

Frame 2

QLCLPGNKHPPSKW*MWVS*PCWSLSPGLPSAS PHCEWSVKHSVDQT*TCLLPGQGGADTSQSTRP GRTRTPRTV*PTCESPGG*CGPGSPGERYECIP GEPVLGLQHAVRHGAPPAEWPACPQPASGFRPSS VHPATGVPEAEGAALPACAHPRCQAPSGGRPAV *NLISAGHPQSFS*TLWPWEPPCAHAGFFTSEP GRRKAGC*SL**RWDKQSGGDSSRHDSQRPVPIA GLQKSLCG*QQLDTSGAPPAPRIREVLGRP*AG GPGGEYHGQ*E*ISIQEELRKIRVL*KSHEFLP RTDGYLVPAVKWQSNPAFAEFSELQ*LS*NSRVF ACERAGKEIMEKAVCVFAEIWPLLLHQGNFKGT QTPAAAGRPGGQQHLLPDRWQEAVQRPYRPRAL HKAKQSQE*N*RAEVALCRGRANQDVLDDSVQTP QVWNAPLPELPNPSAEEGLAVPVLDASAQCLRE LPRGNGFFWANRTRDRESGRGPERSPGGGPRLE EAKHTDEHPR*PKSPPPFYPKYSDSQDTALVSRE DLQGGIPQDH*TARARGWAFSPP*QPE*SKGIC THTVSSPEN*KFPDLTLRGRRADVLQPR*REHQ IL*PDPAG*LLPAEQRSPALQTQAPLHPSGLMTA DVLSAEDWRK*TLE*RSGLCVGEEHTSILHLGT QSEMGLFGASRPRLTSLLDLNDDLLL*TQQGRL PLHRPNWGGHGRSSGKLKINWNDHLGLGRLGTRT GEK*LEMNSCPGIILTIKTDMFTFFVLITFLHS FFVFNIVFSLL*EGDVAFQLI*YRKSFEWADFK LNMGPQMFPEGPPHPLPTTTVWIQLPNDKPSPSQ YT*KAFLLK*KVSLW*AFGIFCGLSQATTVC*S FLYAQMSDPLVISVSCSAQCRRLYSFGKFTVPQ TAG*MASPLD*RGPASLRHTWHPSSASGGCCIIH LGTSALRFHSLQPVLERSLPYPCCCSGVSRFTA DVPRGIKGSKEKMAPGCLRAVSHGFP***RQGN AAGATGTG*YLKIFACSPPSC*KPP*ASEHRAAR GSSSAGLGSEVPHSGCGHGRVLSTQHFVPAPGD ESDGGLSCHLVPSAPRVAPFATSP*LSDQV*EW PFPYL*SL*SRKDSFDRRQTTVVSRMISFFKTTP AWLLLMCAGHDIAPNPPH*SVP*GTAFLCSSVN PRSLEQCHKLQ*GQSAGSQSTV**HCMSVYRPH SSCFP*KVYK*RIF*QNMFLTISAQLHFLPFAAS QSFKLLTVR*FSRSHLVAGFTVGTDIAVTPRFL PLHWLEVNPFQGKTTLGFLFSVYVDQSHQCIIS LSHASLQ*TACICSISYL*ICVYLKGEQKLDSDS QFCM*LV*RSLLYFPCRSELLENVNRLHVAADF LRPQLAGLALGGWEFHLRTSPANRGRKDI*LLF LYLIDMNVKGTAQGCFGAC*LCISACDFLF*MKL HVATRTKLRRKTPNALVIRV**VSSVTLGVRVP ECSFVC*TLKKHVPQPRCFVFSFLHLIPDSMTD LCAFLGAGKLAVDL*CHSANCSDCKLAWRV*TFS GNAEGINEETKPLFHVLCFILYIALWLVNLIVN FQVFLYK*GTDVLFLVIRNKH*YSVL

Sequence NM_005311

AAATGTAATTTGAAGAAGGCAGAAGGAACCCATGGCTTTAGCCGGCTGCCCAGATTCCTTTTTGCACCATCCGTACTACCAGGACAAGGTGGAGCAGACA CCTCGCAGTCAACAAGACCCGGCAGGACCAGGACTCCCCGCACAGTCTGACCGACTTGCGAATCACCAGGAGGATGATGTGGACCTGGAAGCCCTGGTGA ACGATATGAATGCATCCCTGGAGAGCCTGTACTCGGCCTGCAGCATGCAGTCAGACACGGTGCCCCTCCTGCAGAATGGCCAGCATGCCCGCAGCCAGCC TCGGGCTTCAGGCCCTCCTCGGTCCATCCAGCCACAGGTGTCCCCGAGGCAGAGGGTGCAGCGCTCCCAGCCTGTGCACATCCTCGCTGTCAGGCGCCTT CAGGAGGAAGACCAGCAGTTTAGAACCTCATCTCTGCCGGCCATCCCCAATCCTTTTCCTGAACTCTGTGGCCCTGGGAGCCCCCCTGTGCTCACGCCGG GTTCTTTACCTCCGAGCCAGGCCGCCGCAAAGCAGGATGTTAAAGTCTTTAGTGAAGATGGGACAAGCAAAGTGGTGGAGATTCTAGCAGACATGACAGC CAGAGACCTGTGCCAATTGCTGGTTTACAAAAGTCACTGTGTGGATGACAACAGCTGGACACTAGTGGAGCACCACCCGCACCTAGGATTAGAGAGGTGC TTGGAAGACCATGAGCTGGTGGTCCAGGTGGAGAGTACCATGGCCAGTGAGAGTAAATTTCTATTCAGGAAGAATTACGCAAAATACGAGTTCTTTAAAA ATCCCATGAATTTCTTCCCAGAACAGATGGTTACTTGGTGCCAGCAGTCAAATGGCAGTCAAACCCAGCTTTTGCAGAATTTTCTGAACTCCAGTAGTTG TCCTGAAATTCAAGGGTTTTTGCATGTGAAAGAGCTGGGAAAGAAATCATGGAAAAAGCTGTATGTGTGTTTGCGGAGATCTGGCCTTTATTGCTCCACC AAGGGAACTTCAAAGGAACCCAGACACCTGCAGCTGCTGGCCGACCTGGAGGACAGCAACATCTTCTCCCTGATCGCTGGCAGGAAGCAGTACAACGCCC CTACAGACCACGGGCTCTGCATAAAGCCAAACAAAGTCAGGAATGAAACTAAAGAGCTGAGGTTGCTCTGTGCAGAGGACGAGCAAACCAGGACGTGCTG GATGACAGCGTTCAGACTCCTCAAGTATGGAATGCTCCTTTACCAGAATTACCGAATCCCTCAGCAGAGGAAGGCCTTGCTGTCCCCGTTCTCGACGCCA GTGCGCAGTGTCTCCGAGAACTCCCTCGTGGCAATGGATTTTTCTGGGCAAACAGGACGCGTGATAGAGAATCCGGCAGAGGCCCAGAGCGCAGCCCTGG AGGAGGGCCACGCCTGGAGGAAGCGAAGCACACGGATGAACATCCTAGGTAGCCAAAGTCCCCTCCACCCTTCTACCCTAAGTACAGTGATTCACAGGAC ACAGCACTGGTTTCACGGGAGGATCTCCAGGGAGGAATCCCACAGGATCATTAAACAGCAAGGGCTCGTGGATGGGCTTTTTCTCCTCCGTGACAGCCAG AGTAATCCAAAGGCATTTGTACTCACACTGTGTCATCACCAGAAAATTAAAAATTTCCAGATCTTACCTTGCGAGGACGACGGGCAGACGTTCTTCAGCC TAGATGACGGGAACACCAAATTCTCTGACCTGATCCAGCTGGTTGACTTTTACCAGCTGAACAAAGGAGTCCTGCCTTGCAAACTCAAGCACCACTGCAT CCGAGTGGCCTTATGACCGCAGATGTCCTCTCGGCTGAAGACTGGAGGAAGTGAACACTGGAGTGAAGAAGCGGTCTGTGCGTTGGTGAAGAACACACAT CGATTCTGCACCTGGGGACCCAGAGCGAGATGGGTTTGTTCGGTGCCAGCCGACCAAGATTGACTAGTTTGTTGGACTTAAACGACGATTTGCTGCTGTG AACCCAGCAGGGTCGCCTCCCTCTGCATCGGCCAAATTGGGGAGGGCATGGAAGATCCAGCGGAAAGTTGAAAATAAACTGGAATGATCATCTTGGCTTG GGCCGCTTAGGAACAAGAACCGGAGAGAAGTGATTGGAAATGAACTCTTGCCCTGGAATAATCTTGACAATTAAAACTGATATGTTTACTTTTTTTGTAT TGATCACTTTTTTGCACTCCTTCTTTGTTTTCAATATTGTATTCAGCCTATTGTAGGAGGGGGATGTGGCGTTTCAACTCATATAATACAGAAAGAGTTT TGAATGGGCAGATTTCAAACTGAATATGGGTCCCCAAATGTTCCCAGAGGGTCCTCCACACCCTCTGCCGACTACCACGGTGTGGATTCAGCTCCCAAAT GACAAACCCAGCCCTTCCCAGTATACTTGAAAAGCTTTCTTGTTAAAATAAAAGGTGTCACTGTGGTAGGCATTTGGCATATTTTGTGGACTCAGTCAAG CAACCACAGTCTGTTAATCATTTCTCTATGCTCAGATGTCAGATCCTCTTGTTATTAGTGTGTCTTGTTCTGCACAGTGCAGGAGACTTTATTCCTTTGG AAAATTCACTGTTCCACAAACAGCAGGCTGAATGGCCTCGCCTCTAGATTGACGTGGGCCAGCCTCCTTGAGACACACCTGGCACCCGTCATCGGCCAGC GGTGGATGCTGCATAATCCACCTGGGTACTTCAGCCTTGCGTTTCCACAGCCTTCAGCCTGTTCTAGAACGATCACTGCCTTACCCCTGCTGCTGCAGTG GTGTGAGTCGTTTCACGGCTGATGTCCCTCGGGGGATTAAAGGATCTAAAGAGAAAATGGCACCTGGTTGTCTTCGTGCTGTGTCTCATGGGTTTCCATA GTGATAAAGACAAGGAAACGCTGCAGGGGCCACAGGCACAGGCTGATATTTAAAGATCTTTGCTTGCAGCCCTCCGTCCTGCTGAAAACCCCCATAAGCC AGTGAACACAGAGCAGCTAGAGGCTCCTCCTCTGCTGGCTTAGGGTCAGAAGTACCTCACAGTGGTTGTGGACATGGAAGAGTTTTGTCAACACAACACT TTGTCCCCGCTCCGGGAGATGAGTCAGATGGTGGCTTGAGTTGTCACTTGGTCCCCTCCGCCCCTCGGGTGGCCCCCTTTGCCACGTCCCCTTAGCTTAG TGATCAGGTGTGAGAGTGGCCATTTCCTTACCTTTGATCCCTGTAAAGCAGAAAGGACTCCTTTGACAGGCGACAAACTACTGTGGTGAGCAGAATGATT TCCTTTTTCAAGACAACACCTGCCTGGCTTCTATTAATGTGTGCTGGCCATGATATTGCCCCAAATCCGCCCCACTGAAGTGTTCCCTAAGGAACAGCAT TTCTCTGCTCCTCAGTCAACCCCCGTAGCCTAGAGCAGTGTCACAAGCTTCAGTAAGGCCAGTCAGCTGGAAGTCAGTCTACCGTATAGTAACACTGTAT GTCAGTCTACAGACCACACTCTAGTTGTTTTCCATGAAAGGTATACAAATGAAGAATTTTCTAGCAAAACATGTTTTTAACCATCAGTGCTCAATTGCAT TTTCTTCCTTTCGCAGCCAGTCAGTCTTTCAAACTATTGACAGTAAGATAATTCTCACGTTCACACCTGGTGGCAGGCTTCACTGTAGGGACGGACATTG CAGTTACACCACGATTCCTTCCTCTTCACTGGCTCGAGGTAAACCCTTTTCAAGGAAAAACAACTCTAGGATTTCTTTTTTCTGTGTACGTAGACCAGTC CCATCAGTGTATAATCTCTCTCTCACACGCCTCTCTCCAATAGACAGCTTGTATTTGCAGTATTTCATATTTATAAATATGCGTTTATTTAAAAGGAGAA CAAAAGCTTGACTCTGATTCACAGTTTTGTATGTAGCTGGTTTGACGTAGTCTTTTGTATTTTCCCTGCCGAAGTGAATTGTTGGAGAATGTAAACCGCC TCCACGTGGCGGCAGACTTCCTAAGGCCCCAGCTCGCTGGCCTCGCGCTGGGCGGCTGGGAATTCCACCTGAGAACAAGTCCCGCAAACCGGGGACGGAA GGACATTTGACTTTTATTTTTGTATTTAATTGACATGAATGTAAAGGGGACAGCTCAGGGTTGTTTTGGAGCCTGTTGACTTTGTATCTCTGCCTGTGAT TTTCTTTTCTAAATGAAACTCCATGTAGCAACCAGGACGAAGTTGAGAAGGAAAACGCCAAATGCTTTGGTTATTAGAGTTTAATAGGTAAGCTCTGTTA CACTAGGTGTTAGAGTTCCAGAATGTTCTTTTGTTTGCTAAACCTTGAAGAAACATGTGCCTCAGCCTAGATGTTTTGTCTTCTCTTTTCTGCACTTAAT ACCTGACAGTATGACCGATCTCTGCGCCTTTCTGGGGGCGGGCAAGCTGGCGGTAGATTTGTGATGTCACAGTGCAAACTGCAGTGACTGTAAATTGGCC TGGCGTGTATAAACGTTTTCAGGGAATGCAGAAGGTATTAATGAAGAGACAAAACCTTTATTCCATGTGCTTTGCTTCATTCTGTACATAGCTCTTTGGC TCGTGAACCTAATTGTAAACTTTCAGGTATTTTTGTACAAATAAGGGACTGATGTTCTGTTTCTTGTAATTAGAAATAAACATTAATACAGTGTTCTTCA

refseq_GRB10.F1 (forward primer)
refseq_GRB10.R1 (reverse primer)
Single exon skipping

Frame 0

KCNLKKAEGTHGFSRLPRFLFAPSVLPGQGGAD TSQSTRPGRTRTPRTV*PTCESPGG*CGPGSPGE RYECIPGEPVLGLQHAVRHGAPPAEWPACPQPA SGFRPSSVHPATGVPEAEGAALPACAHPRCQAP SGGRPAV*NLISAGHPQSFS*TLWPWEPPCAHAG FFTSEPGRRKAGC*SL**RWDKQSGGDSSRHDS QRPVPIAGLQKSLCG*QQLDTSGAPPAPRIREV LGRP*AGGPGGEYHGQ*E*ISIQEELRKIRVL*K SHEFLPRTDGYLVPAVKWQSNPAFAEFSELQ*L S*NSRVFACERAGKEIMEKAVCVFAEIWPLLLH QGNFKGTQTPAAAGRPGGQQHLLPDRWQEAVQRP YRPRALHKAKQSQE*N*RAEVALCRGRANQDVL DDSVQTPQVWNAPLPELPNPSAEEGLAVPVLDA SAQCLRELPRGNGFFWANRTRDRESGRGPERSPG GGPRLEEAKHTDEHPR*PKSPPPFYPKYSDSQD TALVSREDLQGGIPQDH*TARARGWAFSPP*QP E*SKGICTHTVSSPEN*KFPDLTLRGRRADVLQP R*REHQIL*PDPAG*LLPAEQRSPALQTQAPLH PSGLMTADVLSAEDWRK*TLE*RSGLCVGEEHT SILHLGTQSEMGLFGASRPRLTSLLDLNDDLLL* TQQGRLPLHRPNWGGHGRSSGKLKINWNDHLGL GRLGTRTGEK*LEMNSCPGIILTIKTDMFTFFV LITFLHSFFVFNIVFSLL*EGDVAFQLI*YRKSF EWADFKLNMGPQMFPEGPPHPLPTTTVWIQLPN DKPSPSQYT*KAFLLK*KVSLW*AFGIFCGLSQ ATTVC*SFLYAQMSDPLVISVSCSAQCRRLYSFG KFTVPQTAG*MASPLD*RGPASLRHTWHPSSAS GGCCIIHLGTSALRFHSLQPVLERSLPYPCCCS GVSRFTADVPRGIKGSKEKMAPGCLRAVSHGFP* **RQGNAAGATGTG*YLKIFACSPPSC*KPP*A SEHRAARGSSSAGLGSEVPHSGCGHGRVLSTQH FVPAPGDESDGGLSCHLVPSAPRVAPFATSP*LS DQV*EWPFPYL*SL*SRKDSFDRRQTTVVSRMI SFFKTTPAWLLLMCAGHDIAPNPPH*SVP*GTA FLCSSVNPRSLEQCHKLQ*GQSAGSQSTV**HCM SVYRPHSSCFP*KVYK*RIF*QNMFLTISAQLH FLPFAASQSFKLLTVR*FSRSHLVAGFTVGTDI AVTPRFLPLHWLEVNPFQGKTTLGFLFSVYVDQS HQCIISLSHASLQ*TACICSISYL*ICVYLKGE QKLDSDSQFCM*LV*RSLLYFPCRSELLENVNR LHVAADFLRPQLAGLALGGWEFHLRTSPANRGRK DI*LLFLYLIDMNVKGTAQGCFGAC*LCISACD FLF*MKLHVATRTKLRRKTPNALVIRV**VSSV TLGVRVPECSFVC*TLKKHVPQPRCFVFSFLHLI PDSMTDLCAFLGAGKLAVDL*CHSANCSDCKLA WRV*TFSGNAEGINEETKPLFHVLCFILYIALW LVNLIVNFQVFLYK*GTDVLFLVIRNKH*YSVL

Frame 1

NVI*RRQKEPMALAGCPDSFLHHPYYQDKVEQT PRSQQDPAGPGLPAQSDRLANHQEDDVDLEALV NDMNASLESLYSACSMQSDTVPLLQNGQHARSQP RASGPPRSIQPQVSPRQRVQRSQPVHILAVRRL QEEDQQFRTSSLPAIPNPFPELCGPGSPPVLTP GSLPPSQAAAKQDVKVFSEDGTSKVVEILADMTA RDLCQLLVYKSHCVDDNSWTLVEHHPHLGLERC LEDHELVVQVESTMASESKFLFRKNYAKYEFFK NPMNFFPEQMVTWCQQSNGSQTQLLQNFLNSSSC PEIQGFLHVKELGKKSWKKLYVCLRRSGLYCST KGTSKEPRHLQLLADLEDSNIFSLIAGRKQYNA PTDHGLCIKPNKVRNETKELRLLCAEDEQTRTCW MTAFRLLKYGMLLYQNYRIPQQRKALLSPFSTP VRSVSENSLVAMDFSGQTGRVIENPAEAQSAAL EEGHAWRKRSTRMNILGSQSPLHPSTLSTVIHRT QHWFHGRISREESHRIIKQQGLVDGLFLLRDSQ SNPKAFVLTLCHHQKIKNFQILPCEDDGQTFFS LDDGNTKFSDLIQLVDFYQLNKGVLPCKLKHHCI RVAL*PQMSSRLKTGGSEHWSEEAVCALVKNTH RFCTWGPRARWVCSVPADQD*LVCWT*TTICCC EPSRVASLCIGQIGEGMEDPAES*K*TGMIILAW AA*EQEPERSDWK*TLALE*S*QLKLICLLFLY *SLFCTPSLFSILYSAYCRRGMWRFNSYNTERV LNGQISN*IWVPKCSQRVLHTLCRLPRCGFSSQM TNPALPSILEKLSC*NKRCHCGRHLAYFVDSVK QPQSVNHFSMLRCQILLLLVCLVLHSAGDFIPL ENSLFHKQQAEWPRL*IDVGQPP*DTPGTRHRPA VDAA*STWVLQPCVSTAFSLF*NDHCLTPAAAV V*VVSRLMSLGGLKDLKRKWHLVVFVLCLMGFH SDKDKETLQGPQAQADI*RSLLAALRPAENPHKP VNTEQLEAPPLLA*GQKYLTVVVDMEEFCQHNT LSPLREMSQMVA*VVTWSPPPLGWPPLPRPLSL VIRCESGHFLTFDPCKAERTPLTGDKLLW*AE*F PFSRQHLPGFY*CVLAMILPQIRPTEVFPKEQH FSAPQSTPVA*SSVTSFSKASQLEVSLPYSNTV CQSTDHTLVVFHERYTNEEFSSKTCF*PSVLNCI FFLSQPVSLSNY*Q*DNSHVHTWWQASL*GRTL QLHHDSFLFTGSR*TLFKEKQL*DFFFLCT*TS PISV*SLSHTPLSNRQLVFAVFHIYKYAFI*KEN KSLTLIHSFVCSWFDVVFCIFPAEVNCWRM*TA STWRQTS*GPSSLASRWAAGNST*EQVPQTGDG RTFDFYFCI*LT*M*RGQLRVVLEPVDFVSLPVI FFSK*NSM*QPGRS*EGKRQMLWLLEFNR*ALL H*VLEFQNVLLFAKP*RNMCLSLDVLSSLFCT* YLTV*PISAPFWGRASWR*ICDVTVQTAVTVNWP GVYKRFQGMQKVLMKRQNLYSMCFASFCT*LFG S*T*L*TFRYFCTNKGLMFCFL*LEININTVFF

Frame 2

M*FEEGRRNPWL*PAAQIPFCTIRTTRTRWSRH LAVNKTRQDQDSPHSLTDLRITRRMMWTWKPW* TI*MHPWRACTRPAACSQTRCPSCRMASMPAAS LGLQALLGPSSHRCPRGRGCSAPSLCTSSLSGAF RRKTSSLEPHLCRPSPILFLNSVALGAPLCSRR VLYLRARPPQSRMLKSLVKMGQAKWWRF*QT*Q PETCANCWFTKVTVWMTTAGH*WSTTRT*D*RGA WKTMSWWSRWRVPWPVRVNFYSGRITQNTSSLK IP*ISSQNRWLLGASSQMAVKPSFCRIF*TPVV VLKFKGFCM*KSWERNHGKSCMCVCGDLAFIAPP RELQRNPDTCSCWPTWRTATSSP*SLAGSSTTP LQTTGSA*SQTKSGMKLKS*GCSVQRTSKPGRA G*QRSDSSSMECSFTRITESLSRGRPCCPRSRRQ CAVSPRTPSWQWIFLGKQDA**RIRQRPRAQPW RRATPGGSEAHG*TS*VAKVPSTLLP*VQ*FTG HSTGFTGGSPGRNPTGSLNSKGSWMGFFSSVTAR VIQRHLYSHCVITRKLKISRSYLARTTGRRSSA *MTGTPNSLT*SSWLTFTS*TKESCLANSSTTA SEWPYDRRCPLG*RLEEVNTGVKKRSVRW*RTHI DSAPGDPERDGFVRCQPTKID*FVGLKRRFAAV NPAGSPPSASAKLGRAWKIQRKVENKLE*SSWL GPLRNKNRREVIGNELLPWNNLDN*N*YVYFFCI DHFFALLLCFQYCIQPIVGGGCGVSTHIIQKEF *MGRFQTEYGSPNVPRGSSTPSADYHGVDSAPK *QTQPFPVYLKSFLVKIKGVTVVGIWHILWTQSS NHSLLIISLCSDVRSSCY*CVLFCTVQETLFLW KIHCSTNSRLNGLASRLTWASLLETHLAPVIGQ RWMLHNPPGYFSLAFPQPSACSRTITALPLLLQW CESFHG*CPSGD*RI*RENGTWLSSCCVSWVSI VIKTRKRCRGHRHRLIFKDLCLQPSVLLKTPIS Q*TQSS*RLLLCWLRVRSTSQWLWTWKSFVNTTL CPRSGR*VRWWLELSLGPLRPSGGPLCHVPLA* *SGVRVAISLPLIPVKQKGLL*QATNYCGEQND FLFQDNTCLASINVCWP*YCPKSAPLKCSLRNSI SLLLSQPP*PRAVSQASVRPVSWKSVYRIVTLY VSLQTTL*LFSMKGIQMKNFLAKHVFNHQCSIA FSSFRSQSVFQTIDSKIILTFTPGGRLHCRDGHC SYTTIPSSSLARGKPFSRKNNSRISFFCVRRPV PSVYNLSLTRLSPIDSLYLQYFIFINMRLFKRR TKA*L*FTVLYVAGLT*SFVFSLPK*IVGECKPP PRGGRLPKAPARWPRAGRLGIPPENKSRKPGTE GHLTFIFVFN*HECKGDSSGLFWSLLTLYLCL* FSFLNETPCSNQDEVEKENAKCFGY*SLIGKLCY TRC*SSRMFFCLLNLEETCASA*MFCLLFSALN T*QYDRSLRLSGGGQAGGRFVMSQCKLQ*L*IG LACINVFRECRRY**RDKTFIPCALLHSVHSSLA REPNCKLSGIFVQIRD*CSVSCN*K*TLIQCSS