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Sequences

Sequence NM_001040169

GGCGCAGGCAGCGGCTGGGAGCGCAGCGGCAGCGGCGGCAGCAGAAGCTCGGCTCAGCGGCTGGGGGTGGCCGCTCGAATCTGCCAGGGCACCTCGCTCC
CTCGCCTCTGGCAGCGGGACCCTGTGGGCATTGAAATCCAACTCACTCATGCTTATTTCCTGTAATGGACAAACTTGATGCTAATGTGAGTTCTGAGGAG
GGTTTCGGGTCAGTGGAGAAGGTGGTGCTGCTCACGTTTCTCTCGACGGTTATCCTGATGGCCATCTTGGGGAACCTGCTGGTGATGGTGGCTGTGTGCT
GGGACAGGCAGCTCAGGAAAATAAAAACAAATTATTTCATTGTATCTCTTGCTTTTGCGGATCTGCTGGTTTCGGTGCTGGTGATGCCCTTTGGTGCCAT
TGAGCTGGTTCAAGACATCTGGATTTATGGGGAGGTGTTTTGTCTTGTTCGGACATCTCTGGACGTCCTGCTCACAACGGCATCGATTTTTCACCTGTGC
TGCATTTCTCTGGATAGGTATTACGCCATCTGCTGCCAGCCTTTGGTCTATAGGAACAAGATGACCCCTCTGCGCATCGCATTAATGCTGGGAGGCTGCT
GGGTCATCCCCACGTTTATTTCTTTTCTCCCTATAATGCAAGGCTGGAATAACATTGGCATAATTGATTTGATAGAAAAGAGGAAGTTCAACCAGAACTC
TAACTCTACGTACTGTGTCTTCATGGTCAACAAGCCCTACGCCATCACCTGCTCTGTGGTGGCCTTCTACATCCCATTTCTCCTCATGGTGCTGGCCTAT
TACCGCATCTATGTCACAGCTAAGGAGCATGCCCATCAGATCCAGATGTTACAACGGGCAGGAGCCTCCTCCGAGAGCAGGCCTCAGTCGGCAGACCAGC
ATAGCACTCATCGCATGAGGACAGAGACCAAAGCAGCCAAGACCCTGTGCATCATCATGGGTTGCTTCTGCCTCTGCTGGGCACCATTCTTTGTCACCAA
TATTGTGGATCCTTTCATAGACTACACTGTCCCTGGGCAGGTGTGGACTGCTTTCCTCTGGCTCGGCTATATCAATTCCGGGTTGAACCCTTTTCTCTAC
GCCTTCTTGAATAAGTCTTTTAGACGTGCCTTCCTCATCATCCTCTGCTGTGATGATGAGCGCTACCGAAGACCTTCCATTCTGGGCCAGACTGTCCCTT
GTTCAACCACAACCATTAATGGATCCACACATGTACTAAGGTACACCGTTCTGCACAGGGGACATCATCAGGAACTCGAGAAACTGCCCATACACAATGA
CCCAGAA
TCCCTGGAATCATGCTTCTGATTGAGGACATGGCTCACAACTTAGCCATTCATTCGCATTCATGTTTGCATGAACAGGTCACCCTGGCATCAC
TTCTGAACCTCATCACCACCAGTGAGGCATCAGGTAGTAGGGGCTGAGAGCCCAGAGGAGGTACATGGAGGACAGTGTTGG
  • refseq_HTR4.F5 (forward primer)
  • refseq_HTR4.R6 (reverse primer)
  • Single exon skipping

Frame 0

GAGSGWERSGSGGSRSSAQRLGVAARICQGTSL
PRLWQRDPVGIEIQLTHAYFL*WTNLMLM*VLRR
VSGQWRRWCCSRFSRRLS*WPSWGTCW*WWLCA
GTGSSGK*KQIISLYLLLLRICWFRCW*CPLVP
LSWFKTSGFMGRCFVLFGHLWTSCSQRHRFFTCA
AFLWIGITPSAASLWSIGTR*PLCASH*CWEAA
GSSPRLFLFSL*CKAGITLA*LI**KRGSSTRT
LTLRTVSSWSTSPTPSPALWWPSTSHFSSWCWPI
TASMSQLRSMPIRSRCYNGQEPPPRAGLSRQTS
IALIA*GQRPKQPRPCASSWVASASAGHHSLSP
ILWILS*TTLSLGRCGLLSSGSAISIPG*TLFST
PS*ISLLDVPSSSSSAVMMSATEDLPFWARLSL
VQPQPLMDPHMY*GTPFCTGDIIRNSRNCPYTM
TQN
PWNHASD*GHGSQLSHSFAFMFA*TGHPGIT
SEPHHHQ*GIR**GLRAQRRYMEDSV

Frame 1

AQAAAGSAAAAAAAEARLSGWGWPLESARAPRS
LASGSGTLWALKSNSLMLISCNGQT*C*CEF*G
GFRVSGEGGAAHVSLDGYPDGHLGEPAGDGGCVL
GQAAQENKNKLFHCISCFCGSAGFGAGDALWCH
*AGSRHLDLWGGVLSCSDISGRPAHNGIDFSPV
LHFSG*VLRHLLPAFGL*EQDDPSAHRINAGRLL
GHPHVYFFSPYNARLE*HWHN*FDRKEEVQPEL
*LYVLCLHGQQALRHHLLCGGLLHPISPHGAGL
LPHLCHS*GACPSDPDVTTGRSLLREQASVGRPA
*HSSHEDRDQSSQDPVHHHGLLLPLLGTILCHQ
YCGSFHRLHCPWAGVDCFPLARLYQFRVEPFSL
RLLE*VF*TCLPHHPLL***ALPKTFHSGPDCPL
FNHNH*WIHTCTKVHRSAQGTSSGTRETAHTQ*
PR
IPGIMLLIEDMAHNLAIHSHSCLHEQVTLAS
LLNLITTSEASGSRG*EPRGGTWRTVL

Frame 2

RRQRLGAQRQRRQQKLGSAAGGGRSNLPGHLAP
SPLAAGPCGH*NPTHSCLFPVMDKLDANVSSEE
GFGSVEKVVLLTFLSTVILMAILGNLLVMVAVC
WDRQLRKIKTNYFIVSLAFADLLVSVLVMPFGAI
ELVQDIWIYGEVFCLVRTSLDVLLTTASIFHLC
CISLDRYYAICCQPLVYRNKMTPLRIALMLGGC
WVIPTFISFLPIMQGWNNIGIIDLIEKRKFNQNS
NSTYCVFMVNKPYAITCSVVAFYIPFLLMVLAY
YRIYVTAKEHAHQIQMLQRAGASSESRPQSADQ
HSTHRMRTETKAAKTLCIIMGCFCLCWAPFFVTN
IVDPFIDYTVPGQVWTAFLWLGYINSGLNPFLY
AFLNKSFRRAFLIILCCDDERYRRPSILGQTVP
CSTTTINGSTHVLRYTVLHRGHHQELEKLPIHND
PE
SLESCF*LRTWLTT*PFIRIHVCMNRSPWHH
F*TSSPPVRHQVVGAESPEEVHGGQCW

Sequence NM_199453

GGCGCAGGCAGCGGCTGGGAGCGCAGCGGCAGCGGCGGCAGCAGAAGCTCGGCTCAGCGGCTGGGGGTGGCCGCTCGAATCTGCCAGGGCACCTCGCTCC
CTCGCCTCTGGCAGCGGGACCCTGTGGGCATTGAAATCCAACTCACTCATGCTTATTTCCTGTAATGGACAAACTTGATGCTAATGTGAGTTCTGAGGAG
GGTTTCGGGTCAGTGGAGAAGGTGGTGCTGCTCACGTTTCTCTCGACGGTTATCCTGATGGCCATCTTGGGGAACCTGCTGGTGATGGTGGCTGTGTGCT
GGGACAGGCAGCTCAGGAAAATAAAAACAAATTATTTCATTGTATCTCTTGCTTTTGCGGATCTGCTGGTTTCGGTGCTGGTGATGCCCTTTGGTGCCAT
TGAGCTGGTTCAAGACATCTGGATTTATGGGGAGGTGTTTTGTCTTGTTCGGACATCTCTGGACGTCCTGCTCACAACGGCATCGATTTTTCACCTGTGC
TGCATTTCTCTGGATAGGTATTACGCCATCTGCTGCCAGCCTTTGGTCTATAGGAACAAGATGACCCCTCTGCGCATCGCATTAATGCTGGGAGGCTGCT
GGGTCATCCCCACGTTTATTTCTTTTCTCCCTATAATGCAAGGCTGGAATAACATTGGCATAATTGATTTGATAGAAAAGAGGAAGTTCAACCAGAACTC
TAACTCTACGTACTGTGTCTTCATGGTCAACAAGCCCTACGCCATCACCTGCTCTGTGGTGGCCTTCTACATCCCATTTCTCCTCATGGTGCTGGCCTAT
TACCGCATCTATGTCACAGCTAAGGAGCATGCCCATCAGATCCAGATGTTACAACGGGCAGGAGCCTCCTCCGAGAGCAGGCCTCAGTCGGCAGACCAGC
ATAGCACTCATCGCATGAGGACAGAGACCAAAGCAGCCAAGACCCTGTGCATCATCATGGGTTGCTTCTGCCTCTGCTGGGCACCATTCTTTGTCACCAA
TATTGTGGATCCTTTCATAGACTACACTGTCCCTGGGCAGGTGTGGACTGCTTTCCTCTGGCTCGGCTATATCAATTCCGGGTTGAACCCTTTTCTCTAC
GCCTTCTTGAATAAGTCTTTTAGACGTGCCTTCCTCATCATCCTCTGCTGTGATGATGAGCGCTACCGAAGACCTTCCATTCTGGGCCAGACTGTCCCTT
GTTCAACCACAACCATTAATGGATCCACACATGTACTAAGTGGCTGTTCCCCTGTCTCCAGCTTCCTCCTGCTCTTCTGCAATAGACCAGTTCCTGTCTA
ACTCTGAAAGTGCTCT
GTACACCGTTCTGCACAGGGGACATCATCAGGAACTCGAGAAACTGCCCATACACAATGACCCAGAATCCCTGGAATCATGCTT
CTGATTGAGGACATGGCTCACAACTTAGCCATTCATTCGCATTCATGTTTGCATGAACAGGTCACCCTGGCATCACTTCTGAACCTCATCACCACCAGTG
AGGCATCAGGTAGTAGGGGCTGAGAGCCCAGAGGAGGTACATGGAGGACAGTGTTGG
  • refseq_HTR4.F5 (forward primer)
  • refseq_HTR4.R6 (reverse primer)
  • Single exon skipping

Frame 0

GAGSGWERSGSGGSRSSAQRLGVAARICQGTSL
PRLWQRDPVGIEIQLTHAYFL*WTNLMLM*VLRR
VSGQWRRWCCSRFSRRLS*WPSWGTCW*WWLCA
GTGSSGK*KQIISLYLLLLRICWFRCW*CPLVP
LSWFKTSGFMGRCFVLFGHLWTSCSQRHRFFTCA
AFLWIGITPSAASLWSIGTR*PLCASH*CWEAA
GSSPRLFLFSL*CKAGITLA*LI**KRGSSTRT
LTLRTVSSWSTSPTPSPALWWPSTSHFSSWCWPI
TASMSQLRSMPIRSRCYNGQEPPPRAGLSRQTS
IALIA*GQRPKQPRPCASSWVASASAGHHSLSP
ILWILS*TTLSLGRCGLLSSGSAISIPG*TLFST
PS*ISLLDVPSSSSSAVMMSATEDLPFWARLSL
VQPQPLMDPHMY*VAVPLSPASSCSSAIDQFLS
NSESAL
YTVLHRGHHQELEKLPIHNDPESLESCF
*LRTWLTT*PFIRIHVCMNRSPWHHF*TSSPPV
RHQVVGAESPEEVHGGQCW

Frame 1

AQAAAGSAAAAAAAEARLSGWGWPLESARAPRS
LASGSGTLWALKSNSLMLISCNGQT*C*CEF*G
GFRVSGEGGAAHVSLDGYPDGHLGEPAGDGGCVL
GQAAQENKNKLFHCISCFCGSAGFGAGDALWCH
*AGSRHLDLWGGVLSCSDISGRPAHNGIDFSPV
LHFSG*VLRHLLPAFGL*EQDDPSAHRINAGRLL
GHPHVYFFSPYNARLE*HWHN*FDRKEEVQPEL
*LYVLCLHGQQALRHHLLCGGLLHPISPHGAGL
LPHLCHS*GACPSDPDVTTGRSLLREQASVGRPA
*HSSHEDRDQSSQDPVHHHGLLLPLLGTILCHQ
YCGSFHRLHCPWAGVDCFPLARLYQFRVEPFSL
RLLE*VF*TCLPHHPLL***ALPKTFHSGPDCPL
FNHNH*WIHTCTKWLFPCLQLPPALLQ*TSSCL
TLKVL
CTPFCTGDIIRNSRNCPYTMTQNPWNHA
SD*GHGSQLSHSFAFMFA*TGHPGITSEPHHHQ*
GIR**GLRAQRRYMEDSV

Frame 2

RRQRLGAQRQRRQQKLGSAAGGGRSNLPGHLAP
SPLAAGPCGH*NPTHSCLFPVMDKLDANVSSEE
GFGSVEKVVLLTFLSTVILMAILGNLLVMVAVC
WDRQLRKIKTNYFIVSLAFADLLVSVLVMPFGAI
ELVQDIWIYGEVFCLVRTSLDVLLTTASIFHLC
CISLDRYYAICCQPLVYRNKMTPLRIALMLGGC
WVIPTFISFLPIMQGWNNIGIIDLIEKRKFNQNS
NSTYCVFMVNKPYAITCSVVAFYIPFLLMVLAY
YRIYVTAKEHAHQIQMLQRAGASSESRPQSADQ
HSTHRMRTETKAAKTLCIIMGCFCLCWAPFFVTN
IVDPFIDYTVPGQVWTAFLWLGYINSGLNPFLY
AFLNKSFRRAFLIILCCDDERYRRPSILGQTVP
CSTTTINGSTHVLSGCSPVSSFLLLFCNRPVPV*
L*KCS
VHRSAQGTSSGTRETAHTQ*PRIPGIML
LIEDMAHNLAIHSHSCLHEQVTLASLLNLITTS
EASGSRG*EPRGGTWRTVL

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