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Sequences

Sequence NM_000870

GGCGCAGGCAGCGGCTGGGAGCGCAGCGGCAGCGGCGGCAGCAGAAGCTCGGCTCAGCGGCTGGGGGTGGCCGCTCGAATCTGCCAGGGCACCTCGCTCC CTCGCCTCTGGCAGCGGGACCCTGTGGGCATTGAAATCCAACTCACTCATGCTTATTTCCTGTAATGGACAAACTTGATGCTAATGTGAGTTCTGAGGAG GGTTTCGGGTCAGTGGAGAAGGTGGTGCTGCTCACGTTTCTCTCGACGGTTATCCTGATGGCCATCTTGGGGAACCTGCTGGTGATGGTGGCTGTGTGCT GGGACAGGCAGCTCAGGAAAATAAAAACAAATTATTTCATTGTATCTCTTGCTTTTGCGGATCTGCTGGTTTCGGTGCTGGTGATGCCCTTTGGTGCCAT TGAGCTGGTTCAAGACATCTGGATTTATGGGGAGGTGTTTTGTCTTGTTCGGACATCTCTGGACGTCCTGCTCACAACGGCATCGATTTTTCACCTGTGC TGCATTTCTCTGGATAGGTATTACGCCATCTGCTGCCAGCCTTTGGTCTATAGGAACAAGATGACCCCTCTGCGCATCGCATTAATGCTGGGAGGCTGCT GGGTCATCCCCACGTTTATTTCTTTTCTCCCTATAATGCAAGGCTGGAATAACATTGGCATAATTGATTTGATAGAAAAGAGGAAGTTCAACCAGAACTC TAACTCTACGTACTGTGTCTTCATGGTCAACAAGCCCTACGCCATCACCTGCTCTGTGGTGGCCTTCTACATCCCATTTCTCCTCATGGTGCTGGCCTAT TACCGCATCTATGTCACAGCTAAGGAGCATGCCCATCAGATCCAGATGTTACAACGGGCAGGAGCCTCCTCCGAGAGCAGGCCTCAGTCGGCAGACCAGC ATAGCACTCATCGCATGAGGACAGAGACCAAAGCAGCCAAGACCCTGTGCATCATCATGGGTTGCTTCTGCCTCTGCTGGGCACCATTCTTTGTCACCAA TATTGTGGATCCTTTCATAGACTACACTGTCCCTGGGCAGGTGTGGACTGCTTTCCTCTGGCTCGGCTATATCAATTCCGGGTTGAACCCTTTTCTCTAC GCCTTCTTGAATAAGTCTTTTAGACGTGCCTTCCTCATCATCCTCTGCTGTGATGATGAGCGCTACCGAAGACCTTCCATTCTGGGCCAGACTGTCCCTT GTTCAACCACAACCATTAATGGATCCACACATGTACTAAGGGATGCAGTGGAGTGTGGTGGCCAGTGGGAGAGTCAGTGTCACCCGCCAGCAACTTCTCC TTTGGTGGCTGCTCAGCCCAGTGACACTTAGGCCCCTGGGACAATGACCCAGAAGACAGCCATGCCTCCGAAAGAGGGCCAGGTCCTAAGCTGCTGCTTG TGCGCGACTGCACCCGGTATTCTCTTCACCTGAGGCTTTCCGTCCGCCAGTGCAGGAACCCGGTGCTCGCTGGGCTTTTCCTCTGAGATTCCAGCAGGTG GCGCTGGAGGGAGTCAGGGGACATAATGGCCTCCTTGTTCACTTTTTATTTCCCAACACTCCCTCTTCCCAGATTCTCTGCTTTTGCCGCGTGGTCTCTG GTGTCTCTGACATGTTCCCGTCACTCACTGTGTCTGATCTGTCTCACTCACGTTCTAAGCACGATAGCTTGCTGCGTCCTATCTCGGTCACTGATGTCTC CAAATACGCCTTCTTTGCTGTGTGGGCTTCCAGATATCAACAAAATACTCCCTCTCTGTTCCTGTCTTAGCCAGAGGCGTCGCTCCTGTCTTGCTTACTT GCTTGGTCACGTCCGTCACAGCCATGTTCAAGCTATCCCCTGCCTCTTCTTCGCCCTGTTCTGTGCCAGACGCTAACACACTTTTCTCCCTGTGTCTGGG TCCAAACGTGCATCCTCTTGGTTGGCCCCCTTGTCAGTCCCAGACGTCCACTTGCTCTTCCTTATGTCCAATGTCTGTCCTCCTCTTCAGGACATGCTCC ATTTATTTTCCATGGAAGACACAGGGTCAGAGATCTGTCAGAGGAAAATGGTCTCCACAGCAACTCTGGAGGGAAGCTGAAGACAGATAGCTTCCTCCTG GTGTCCTGGCCGCTGGCGTGTCTGCCCTGAGATGCTCGCTCTCCTGCTGGGCTAGAACGGGACGTCTTCTAAAAAGCCTTGGGCAGGGCTAGCAACATCG TAGGTGTTTATTAGGCTTCAGGATCTTTCCCATGAACTTGTGATATCATTTTGAAACCCTTCAATCTGGCTTTGTTGCTGCCAAAGGAGATAACTGTTGG AAGTTCACACTGGGGAAACTCATTTTCCCCTGCAGCTCCCCCAAGGTGCAGGAGGGCAGGATCCAGCAGCACTGGGGATGCCGAGGCAATGCTGTACTTG AAGCCAGAGGACCCAAATTGCCGCCCTGGCTGTGCTGCAAACAAGCTGTGAGATCTTGAATAGTGTTCTTTCATCTCTGGACCCCACTTTCCACATCTGT GCACTCAAGCTTGGACTAGAGGACCTTTAAGTTTTCTTTCTGTTTCTAAGACAACAGTATGCTGCTATAGCCGTCTGCTACCTCGAGAAGCCATGGCCTG CCTGCCTTTTGGCTTTGTTTCAAAGGAACGACCCTCCTCATTTGGGCCTTTTAGACCCCTGACCTTTCCAGCTTTGTCCTTCAGATTAATCTGGATGTCT TTTATTCAAAGCTGAGTCAGTGGCTCTTGCGAATGCCCCATAGCAGAAGTCCCGGGAAGGTAAATGCCAAACCCAGAGCCATGTGAGATTCTGACTTTGA TCTTGGCTGTGGTTTAATAGCATCTCAGGATTATTTATCATATTCTCTTTTTTTCTTATTGCTTATAAGGTGTCAGTTATTGTTTCCATTGTTGTTTACT TTAGTTCTGGAACTGAAACCGACAGGAAGAAACTTTGGAATAAGGAAGAGAAGATTGACCAAACCATCCAAATGCCAAAAA

refseq_HTR4.F1 (forward primer)
refseq_HTR4.R1 (reverse primer)
Single exon skipping

Frame 0

GAGSGWERSGSGGSRSSAQRLGVAARICQGTSL PRLWQRDPVGIEIQLTHAYFL*WTNLMLM*VLRR VSGQWRRWCCSRFSRRLS*WPSWGTCW*WWLCA GTGSSGK*KQIISLYLLLLRICWFRCW*CPLVP LSWFKTSGFMGRCFVLFGHLWTSCSQRHRFFTCA AFLWIGITPSAASLWSIGTR*PLCASH*CWEAA GSSPRLFLFSL*CKAGITLA*LI**KRGSSTRT LTLRTVSSWSTSPTPSPALWWPSTSHFSSWCWPI TASMSQLRSMPIRSRCYNGQEPPPRAGLSRQTS IALIA*GQRPKQPRPCASSWVASASAGHHSLSP ILWILS*TTLSLGRCGLLSSGSAISIPG*TLFST PS*ISLLDVPSSSSSAVMMSATEDLPFWARLSL VQPQPLMDPHMY*GMQWSVVASGRVSVTRQQLL LWWLLSPVTLRPLGQ*PRRQPCLRKRARS*AAAC ARLHPVFSSPEAFRPPVQEPGARWAFPLRFQQV ALEGVRGHNGLLVHFLFPNTPSSQILCFCRVVS GVSDMFPSLTVSDLSHSRSKHDSLLRPISVTDVS KYAFFAVWASRYQQNTPSLFLS*PEASLLSCLL AWSRPSQPCSSYPLPLLRPVLCQTLTHFSPCVW VQTCILLVGPLVSPRRPLALPYVQCLSSSSGHAP FIFHGRHRVRDLSEENGLHSNSGGKLKTDSFLL VSWPLACLP*DARSPAGLERDVF*KALGRASNI VGVY*ASGSFP*TCDIILKPFNLALLLPKEITVG SSHWGNSFSPAAPPRCRRAGSSSTGDAEAMLYL KPEDPNCRPGCAANKL*DLE*CSFISGPHFPHL CTQAWTRGPLSFLSVSKTTVCCYSRLLPREAMAC LPFGFVSKERPSSFGPFRPLTFPALSFRLIWMS FIQS*VSGSCECPIAEVPGR*MPNPEPCEILTL ILAVV**HLRIIYHILFFSYCL*GVSYCFHCCLL *FWN*NRQEETLE*GRED*PNHPNAK

Frame 1

AQAAAGSAAAAAAAEARLSGWGWPLESARAPRS LASGSGTLWALKSNSLMLISCNGQT*C*CEF*G GFRVSGEGGAAHVSLDGYPDGHLGEPAGDGGCVL GQAAQENKNKLFHCISCFCGSAGFGAGDALWCH *AGSRHLDLWGGVLSCSDISGRPAHNGIDFSPV LHFSG*VLRHLLPAFGL*EQDDPSAHRINAGRLL GHPHVYFFSPYNARLE*HWHN*FDRKEEVQPEL *LYVLCLHGQQALRHHLLCGGLLHPISPHGAGL LPHLCHS*GACPSDPDVTTGRSLLREQASVGRPA *HSSHEDRDQSSQDPVHHHGLLLPLLGTILCHQ YCGSFHRLHCPWAGVDCFPLARLYQFRVEPFSL RLLE*VF*TCLPHHPLL***ALPKTFHSGPDCPL FNHNH*WIHTCTKGCSGVWWPVGESVSPASNFS FGGCSAQ*HLGPWDNDPEDSHASERGPGPKLLL VRDCTRYSLHLRLSVRQCRNPVLAGLFL*DSSRW RWRESGDIMASLFTFYFPTLPLPRFSAFAAWSL VSLTCSRHSLCLICLTHVLSTIACCVLSRSLMS PNTPSLLCGLPDINKILPLCSCLSQRRRSCLAYL LGHVRHSHVQAIPCLFFALFCARR*HTFLPVSG SKRASSWLAPLSVPDVHLLFLMSNVCPPLQDML HLFSMEDTGSEICQRKMVSTATLEGS*RQIASSW CPGRWRVCPEMLALLLG*NGTSSKKPWAGLATS *VFIRLQDLSHELVISF*NPSIWLCCCQRR*LL EVHTGETHFPLQLPQGAGGQDPAALGMPRQCCT* SQRTQIAALAVLQTSCEILNSVLSSLDPTFHIC ALKLGLEDL*VFFLFLRQQYAAIAVCYLEKPWP ACLLALFQRNDPPHLGLLDP*PFQLCPSD*SGCL LFKAESVALANAP*QKSREGKCQTQSHVRF*L* SWLWFNSISGLFIIFSFFLIAYKVSVIVSIVVY FSSGTETDRKKLWNKEEKIDQTIQMPK

Frame 2

RRQRLGAQRQRRQQKLGSAAGGGRSNLPGHLAP SPLAAGPCGH*NPTHSCLFPVMDKLDANVSSEE GFGSVEKVVLLTFLSTVILMAILGNLLVMVAVC WDRQLRKIKTNYFIVSLAFADLLVSVLVMPFGAI ELVQDIWIYGEVFCLVRTSLDVLLTTASIFHLC CISLDRYYAICCQPLVYRNKMTPLRIALMLGGC WVIPTFISFLPIMQGWNNIGIIDLIEKRKFNQNS NSTYCVFMVNKPYAITCSVVAFYIPFLLMVLAY YRIYVTAKEHAHQIQMLQRAGASSESRPQSADQ HSTHRMRTETKAAKTLCIIMGCFCLCWAPFFVTN IVDPFIDYTVPGQVWTAFLWLGYINSGLNPFLY AFLNKSFRRAFLIILCCDDERYRRPSILGQTVP CSTTTINGSTHVLRDAVECGGQWESQCHPPATSP LVAAQPSDT*APGTMTQKTAMPPKEGQVLSCCL CATAPGILFT*GFPSASAGTRCSLGFSSEIPAG GAGGSQGT*WPPCSLFISQHSLFPDSLLLPRGLW CL*HVPVTHCV*SVSLTF*AR*LAASYLGH*CL QIRLLCCVGFQISTKYSLSVPVLARGVAPVLLT CLVTSVTAMFKLSPASSSPCSVPDANTLFSLCLG PNVHPLGWPPCQSQTSTCSSLCPMSVLLFRTCS IYFPWKTQGQRSVRGKWSPQQLWREAEDR*LPP GVLAAGVSALRCSLSCWARTGRLLKSLGQG*QHR RCLLGFRIFPMNL*YHFETLQSGFVAAKGDNCW KFTLGKLIFPCSSPKVQEGRIQQHWGCRGNAVL EARGPKLPPWLCCKQAVRS*IVFFHLWTPLSTSV HSSLD*RTFKFSFCF*DNSMLL*PSATSRSHGL PAFWLCFKGTTLLIWAF*TPDLSSFVLQINLDV FYSKLSQWLLRMPHSRSPGKVNAKPRAM*DSDFD LGCGLIASQDYLSYSLFFLLLIRCQLLFPLLFT LVLELKPTGRNFGIRKRRLTKPSKCQK

Sequence NM_001040172

GGCGCAGGCAGCGGCTGGGAGCGCAGCGGCAGCGGCGGCAGCAGAAGCTCGGCTCAGCGGCTGGGGGTGGCCGCTCGAATCTGCCAGGGCACCTCGCTCC CTCGCCTCTGGCAGCGGGACCCTGTGGGCATTGAAATCCAACTCACTCATGCTTATTTCCTGTAATGGACAAACTTGATGCTAATGTGAGTTCTGAGGAG GGTTTCGGGTCAGTGGAGAAGGTGGTGCTGCTCACGTTTCTCTCGACGGTTATCCTGATGGCCATCTTGGGGAACCTGCTGGTGATGGTGGCTGTGTGCT GGGACAGGCAGCTCAGGAAAATAAAAACAAATTATTTCATTGTATCTCTTGCTTTTGCGGATCTGCTGGTTTCGGTGCTGGTGATGCCCTTTGGTGCCAT TGAGCTGGTTCAAGACATCTGGATTTATGGGGAGGTGTTTTGTCTTGTTCGGACATCTCTGGACGTCCTGCTCACAACGGCATCGATTTTTCACCTGTGC TGCATTTCTCTGGATAGGTATTACGCCATCTGCTGCCAGCCTTTGGTCTATAGGAACAAGATGACCCCTCTGCGCATCGCATTAATGCTGGGAGGCTGCT GGGTCATCCCCACGTTTATTTCTTTTCTCCCTATAATGCAAGGCTGGAATAACATTGGCATAATTGATTTGGAAAGGAGTCTAAACCAAGGCCTGGGCCA GGATTTTCATGCGATAGAAAAGAGGAAGTTCAACCAGAACTCTAACTCTACGTACTGTGTCTTCATGGTCAACAAGCCCTACGCCATCACCTGCTCTGTG GTGGCCTTCTACATCCCATTTCTCCTCATGGTGCTGGCCTATTACCGCATCTATGTCACAGCTAAGGAGCATGCCCATCAGATCCAGATGTTACAACGGG CAGGAGCCTCCTCCGAGAGCAGGCCTCAGTCGGCAGACCAGCATAGCACTCATCGCATGAGGACAGAGACCAAAGCAGCCAAGACCCTGTGCATCATCAT GGGTTGCTTCTGCCTCTGCTGGGCACCATTCTTTGTCACCAATATTGTGGATCCTTTCATAGACTACACTGTCCCTGGGCAGGTGTGGACTGCTTTCCTC TGGCTCGGCTATATCAATTCCGGGTTGAACCCTTTTCTCTACGCCTTCTTGAATAAGTCTTTTAGACGTGCCTTCCTCATCATCCTCTGCTGTGATGATG AGCGCTACCGAAGACCTTCCATTCTGGGCCAGACTGTCCCTTGTTCAACCACAACCATTAATGGATCCACACATGTACTAAGGGATGCAGTGGAGTGTGG TGGCCAGTGGGAGAGTCAGTGTCACCCGCCAGCAACTTCTCCTTTGGTGGCTGCTCAGCCCAGTGACACTTAGGCCCCTGGGACAATGACCCAGAAGACA GCCATGCCTCCGAAAGAGGGCCAGGTCCTAAGCTGCTGCTTGTGCGCGACTGCACCCGGTATTCTCTTCACCTGAGGCTTTCCGTCCGCCAGTGCAGGAA CCCGGTGCTCGCTGGGCTTTTCCTCTGAGATTCCAGCAGGTGGCGCTGGAGGGAGTCAGGGGACATAATGGCCTCCTTGTTCACTTTTTATTTCCCAACA CTCCCTCTTCCCAGATTCTCTGCTTTTGCCGCGTGGTCTCTGGTGTCTCTGACATGTTCCCGTCACTCACTGTGTCTGATCTGTCTCACTCACGTTCTAA GCACGATAGCTTGCTGCGTCCTATCTCGGTCACTGATGTCTCCAAATACGCCTTCTTTGCTGTGTGGGCTTCCAGATATCAACAAAATACTCCCTCTCTG TTCCTGTCTTAGCCAGAGGCGTCGCTCCTGTCTTGCTTACTTGCTTGGTCACGTCCGTCACAGCCATGTTCAAGCTATCCCCTGCCTCTTCTTCGCCCTG TTCTGTGCCAGACGCTAACACACTTTTCTCCCTGTGTCTGGGTCCAAACGTGCATCCTCTTGGTTGGCCCCCTTGTCAGTCCCAGACGTCCACTTGCTCT TCCTTATGTCCAATGTCTGTCCTCCTCTTCAGGACATGCTCCATTTATTTTCCATGGAAGACACAGGGTCAGAGATCTGTCAGAGGAAAATGGTCTCCAC AGCAACTCTGGAGGGAAGCTGAAGACAGATAGCTTCCTCCTGGTGTCCTGGCCGCTGGCGTGTCTGCCCTGAGATGCTCGCTCTCCTGCTGGGCTAGAAC GGGACGTCTTCTAAAAAGCCTTGGGCAGGGCTAGCAACATCGTAGGTGTTTATTAGGCTTCAGGATCTTTCCCATGAACTTGTGATATCATTTTGAAACC CTTCAATCTGGCTTTGTTGCTGCCAAAGGAGATAACTGTTGGAAGTTCACACTGGGGAAACTCATTTTCCCCTGCAGCTCCCCCAAGGTGCAGGAGGGCA GGATCCAGCAGCACTGGGGATGCCGAGGCAATGCTGTACTTGAAGCCAGAGGACCCAAATTGCCGCCCTGGCTGTGCTGCAAACAAGCTGTGAGATCTTG AATAGTGTTCTTTCATCTCTGGACCCCACTTTCCACATCTGTGCACTCAAGCTTGGACTAGAGGACCTTTAAGTTTTCTTTCTGTTTCTAAGACAACAGT ATGCTGCTATAGCCGTCTGCTACCTCGAGAAGCCATGGCCTGCCTGCCTTTTGGCTTTGTTTCAAAGGAACGACCCTCCTCATTTGGGCCTTTTAGACCC CTGACCTTTCCAGCTTTGTCCTTCAGATTAATCTGGATGTCTTTTATTCAAAGCTGAGTCAGTGGCTCTTGCGAATGCCCCATAGCAGAAGTCCCGGGAA GGTAAATGCCAAACCCAGAGCCATGTGAGATTCTGACTTTGATCTTGGCTGTGGTTTAATAGCATCTCAGGATTATTTATCATATTCTCTTTTTTTCTTA TTGCTTATAAGGTGTCAGTTATTGTTTCCATTGTTGTTTACTTTAGTTCTGGAACTGAAACCGACAGGAAGAAACTTTGGAATAAGGAAGAGAAGATTGA CCAAACCATCCAAATGCCAAAAA

refseq_HTR4.F1 (forward primer)
refseq_HTR4.R1 (reverse primer)
Single exon skipping

Frame 0

GAGSGWERSGSGGSRSSAQRLGVAARICQGTSL PRLWQRDPVGIEIQLTHAYFL*WTNLMLM*VLRR VSGQWRRWCCSRFSRRLS*WPSWGTCW*WWLCA GTGSSGK*KQIISLYLLLLRICWFRCW*CPLVP LSWFKTSGFMGRCFVLFGHLWTSCSQRHRFFTCA AFLWIGITPSAASLWSIGTR*PLCASH*CWEAA GSSPRLFLFSL*CKAGITLA*LIWKGV*TKAWA RIFMR*KRGSSTRTLTLRTVSSWSTSPTPSPALW WPSTSHFSSWCWPITASMSQLRSMPIRSRCYNG QEPPPRAGLSRQTSIALIA*GQRPKQPRPCASS WVASASAGHHSLSPILWILS*TTLSLGRCGLLSS GSAISIPG*TLFSTPS*ISLLDVPSSSSSAVMM SATEDLPFWARLSLVQPQPLMDPHMY*GMQWSV VASGRVSVTRQQLLLWWLLSPVTLRPLGQ*PRRQ PCLRKRARS*AAACARLHPVFSSPEAFRPPVQE PGARWAFPLRFQQVALEGVRGHNGLLVHFLFPN TPSSQILCFCRVVSGVSDMFPSLTVSDLSHSRSK HDSLLRPISVTDVSKYAFFAVWASRYQQNTPSL FLS*PEASLLSCLLAWSRPSQPCSSYPLPLLRP VLCQTLTHFSPCVWVQTCILLVGPLVSPRRPLAL PYVQCLSSSSGHAPFIFHGRHRVRDLSEENGLH SNSGGKLKTDSFLLVSWPLACLP*DARSPAGLE RDVF*KALGRASNIVGVY*ASGSFP*TCDIILKP FNLALLLPKEITVGSSHWGNSFSPAAPPRCRRA GSSSTGDAEAMLYLKPEDPNCRPGCAANKL*DL E*CSFISGPHFPHLCTQAWTRGPLSFLSVSKTTV CCYSRLLPREAMACLPFGFVSKERPSSFGPFRP LTFPALSFRLIWMSFIQS*VSGSCECPIAEVPG R*MPNPEPCEILTLILAVV**HLRIIYHILFFSY CL*GVSYCFHCCLL*FWN*NRQEETLE*GRED* PNHPNAK

Frame 1

AQAAAGSAAAAAAAEARLSGWGWPLESARAPRS LASGSGTLWALKSNSLMLISCNGQT*C*CEF*G GFRVSGEGGAAHVSLDGYPDGHLGEPAGDGGCVL GQAAQENKNKLFHCISCFCGSAGFGAGDALWCH *AGSRHLDLWGGVLSCSDISGRPAHNGIDFSPV LHFSG*VLRHLLPAFGL*EQDDPSAHRINAGRLL GHPHVYFFSPYNARLE*HWHN*FGKESKPRPGP GFSCDRKEEVQPEL*LYVLCLHGQQALRHHLLC GGLLHPISPHGAGLLPHLCHS*GACPSDPDVTTG RSLLREQASVGRPA*HSSHEDRDQSSQDPVHHH GLLLPLLGTILCHQYCGSFHRLHCPWAGVDCFP LARLYQFRVEPFSLRLLE*VF*TCLPHHPLL*** ALPKTFHSGPDCPLFNHNH*WIHTCTKGCSGVW WPVGESVSPASNFSFGGCSAQ*HLGPWDNDPED SHASERGPGPKLLLVRDCTRYSLHLRLSVRQCRN PVLAGLFL*DSSRWRWRESGDIMASLFTFYFPT LPLPRFSAFAAWSLVSLTCSRHSLCLICLTHVL STIACCVLSRSLMSPNTPSLLCGLPDINKILPLC SCLSQRRRSCLAYLLGHVRHSHVQAIPCLFFAL FCARR*HTFLPVSGSKRASSWLAPLSVPDVHLL FLMSNVCPPLQDMLHLFSMEDTGSEICQRKMVST ATLEGS*RQIASSWCPGRWRVCPEMLALLLG*N GTSSKKPWAGLATS*VFIRLQDLSHELVISF*N PSIWLCCCQRR*LLEVHTGETHFPLQLPQGAGGQ DPAALGMPRQCCT*SQRTQIAALAVLQTSCEIL NSVLSSLDPTFHICALKLGLEDL*VFFLFLRQQ YAAIAVCYLEKPWPACLLALFQRNDPPHLGLLDP *PFQLCPSD*SGCLLFKAESVALANAP*QKSRE GKCQTQSHVRF*L*SWLWFNSISGLFIIFSFFL IAYKVSVIVSIVVYFSSGTETDRKKLWNKEEKID QTIQMPK

Frame 2

RRQRLGAQRQRRQQKLGSAAGGGRSNLPGHLAP SPLAAGPCGH*NPTHSCLFPVMDKLDANVSSEE GFGSVEKVVLLTFLSTVILMAILGNLLVMVAVC WDRQLRKIKTNYFIVSLAFADLLVSVLVMPFGAI ELVQDIWIYGEVFCLVRTSLDVLLTTASIFHLC CISLDRYYAICCQPLVYRNKMTPLRIALMLGGC WVIPTFISFLPIMQGWNNIGIIDLERSLNQGLGQ DFHAIEKRKFNQNSNSTYCVFMVNKPYAITCSV VAFYIPFLLMVLAYYRIYVTAKEHAHQIQMLQR AGASSESRPQSADQHSTHRMRTETKAAKTLCIIM GCFCLCWAPFFVTNIVDPFIDYTVPGQVWTAFL WLGYINSGLNPFLYAFLNKSFRRAFLIILCCDD ERYRRPSILGQTVPCSTTTINGSTHVLRDAVECG GQWESQCHPPATSPLVAAQPSDT*APGTMTQKT AMPPKEGQVLSCCLCATAPGILFT*GFPSASAG TRCSLGFSSEIPAGGAGGSQGT*WPPCSLFISQH SLFPDSLLLPRGLWCL*HVPVTHCV*SVSLTF* AR*LAASYLGH*CLQIRLLCCVGFQISTKYSLS VPVLARGVAPVLLTCLVTSVTAMFKLSPASSSPC SVPDANTLFSLCLGPNVHPLGWPPCQSQTSTCS SLCPMSVLLFRTCSIYFPWKTQGQRSVRGKWSP QQLWREAEDR*LPPGVLAAGVSALRCSLSCWART GRLLKSLGQG*QHRRCLLGFRIFPMNL*YHFET LQSGFVAAKGDNCWKFTLGKLIFPCSSPKVQEG RIQQHWGCRGNAVLEARGPKLPPWLCCKQAVRS* IVFFHLWTPLSTSVHSSLD*RTFKFSFCF*DNS MLL*PSATSRSHGLPAFWLCFKGTTLLIWAF*T PDLSSFVLQINLDVFYSKLSQWLLRMPHSRSPGK VNAKPRAM*DSDFDLGCGLIASQDYLSYSLFFL LLIRCQLLFPLLFTLVLELKPTGRNFGIRKRRL TKPSKCQK

Sequence NM_001040173

GGCGCAGGCAGCGGCTGGGAGCGCAGCGGCAGCGGCGGCAGCAGAAGCTCGGCTCAGCGGCTGGGGGTGGCCGCTCGAATCTGCCAGGGCACCTCGCTCC CTCGCCTCTGGCAGCGGGACCCTGTGGGCATTGAAATCCAACTCACTCATGCTTATTTCCTGTAATGGACAAACTTGATGCTAATGTGAGTTCTGAGGAG GGTTTCGGGTCAGTGGAGAAGGTGGTGCTGCTCACGTTTCTCTCGACGGTTATCCTGATGGCCATCTTGGGGAACCTGCTGGTGATGGTGGCTGTGTGCT GGGACAGGCAGCTCAGGAAAATAAAAACAAATTATTTCATTGTATCTCTTGCTTTTGCGGATCTGCTGGTTTCGGTGCTGGTGATGCCCTTTGGTGCCAT TGAGCTGGTTCAAGACATCTGGATTTATGGGGAGGTGTTTTGTCTTGTTCGGACATCTCTGGACGTCCTGCTCACAACGGCATCGATTTTTCACCTGTGC TGCATTTCTCTGGATAGGTATTACGCCATCTGCTGCCAGCCTTTGGTCTATAGGAACAAGATGACCCCTCTGCGCATCGCATTAATGCTGGGAGGCTGCT GGGTCATCCCCACGTTTATTTCTTTTCTCCCTATAATGCAAGGCTGGAATAACATTGGCATAATTGATTTGATAGAAAAGAGGAAGTTCAACCAGAACTC TAACTCTACGTACTGTGTCTTCATGGTCAACAAGCCCTACGCCATCACCTGCTCTGTGGTGGCCTTCTACATCCCATTTCTCCTCATGGTGCTGGCCTAT TACCGCATCTATGTCACAGCTAAGGAGCATGCCCATCAGATCCAGATGTTACAACGGGCAGGAGCCTCCTCCGAGAGCAGGCCTCAGTCGGCAGACCAGC ATAGCACTCATCGCATGAGGACAGAGACCAAAGCAGCCAAGACCCTGTGCATCATCATGGGTTGCTTCTGCCTCTGCTGGGCACCATTCTTTGTCACCAA TATTGTGGATCCTTTCATAGACTACACTGTCCCTGGGCAGGTGTGGACTGCTTTCCTCTGGCTCGGCTATATCAATTCCGGGTTGAACCCTTTTCTCTAC GCCTTCTTGAATAAGTCTTTTAGACGTGCCTTCCTCATCATCCTCTGCTGTGATGATGAGCGCTACCGAAGACCTTCCATTCTGGGCCAGACTGTCCCTT GTTCAACCACAACCATTAATGGATCCACACATGTACTAAGGACAGATTTTCTGTTTGACAGAGACATTTTAGCAAGGTATTGGACAAAGCCTGCCAGAGC GGGTCCATTCTCAGGCACATTGAGCATTAGATGTTTGACTGCAAGGAAGCCTGTCTTGGGGGATGCAGTGGAGTGTGGTGGCCAGTGGGAGAGTCAGTGT CACCCGCCAGCAACTTCTCCTTTGGTGGCTGCTCAGCCCAGTGACACTTAGGCCCCTGGGACAATGACCCAGAAGACAGCCATGCCTCCGAAAGAGGGCC AGGTCCTAAGCTGCTGCTTGTGCGCGACTGCACCCGGTATTCTCTTCACCTGAGGCTTTCCGTCCGCCAGTGCAGGAACCCGGTGCTCGCTGGGCTTTTC CTCTGAGATTCCAGCAGGTGGCGCTGGAGGGAGTCAGGGGACATAATGGCCTCCTTGTTCACTTTTTATTTCCCAACACTCCCTCTTCCCAGATTCTCTG CTTTTGCCGCGTGGTCTCTGGTGTCTCTGACATGTTCCCGTCACTCACTGTGTCTGATCTGTCTCACTCACGTTCTAAGCACGATAGCTTGCTGCGTCCT ATCTCGGTCACTGATGTCTCCAAATACGCCTTCTTTGCTGTGTGGGCTTCCAGATATCAACAAAATACTCCCTCTCTGTTCCTGTCTTAGCCAGAGGCGT CGCTCCTGTCTTGCTTACTTGCTTGGTCACGTCCGTCACAGCCATGTTCAAGCTATCCCCTGCCTCTTCTTCGCCCTGTTCTGTGCCAGACGCTAACACA CTTTTCTCCCTGTGTCTGGGTCCAAACGTGCATCCTCTTGGTTGGCCCCCTTGTCAGTCCCAGACGTCCACTTGCTCTTCCTTATGTCCAATGTCTGTCC TCCTCTTCAGGACATGCTCCATTTATTTTCCATGGAAGACACAGGGTCAGAGATCTGTCAGAGGAAAATGGTCTCCACAGCAACTCTGGAGGGAAGCTGA AGACAGATAGCTTCCTCCTGGTGTCCTGGCCGCTGGCGTGTCTGCCCTGAGATGCTCGCTCTCCTGCTGGGCTAGAACGGGACGTCTTCTAAAAAGCCTT GGGCAGGGCTAGCAACATCGTAGGTGTTTATTAGGCTTCAGGATCTTTCCCATGAACTTGTGATATCATTTTGAAACCCTTCAATCTGGCTTTGTTGCTG CCAAAGGAGATAACTGTTGGAAGTTCACACTGGGGAAACTCATTTTCCCCTGCAGCTCCCCCAAGGTGCAGGAGGGCAGGATCCAGCAGCACTGGGGATG CCGAGGCAATGCTGTACTTGAAGCCAGAGGACCCAAATTGCCGCCCTGGCTGTGCTGCAAACAAGCTGTGAGATCTTGAATAGTGTTCTTTCATCTCTGG ACCCCACTTTCCACATCTGTGCACTCAAGCTTGGACTAGAGGACCTTTAAGTTTTCTTTCTGTTTCTAAGACAACAGTATGCTGCTATAGCCGTCTGCTA CCTCGAGAAGCCATGGCCTGCCTGCCTTTTGGCTTTGTTTCAAAGGAACGACCCTCCTCATTTGGGCCTTTTAGACCCCTGACCTTTCCAGCTTTGTCCT TCAGATTAATCTGGATGTCTTTTATTCAAAGCTGAGTCAGTGGCTCTTGCGAATGCCCCATAGCAGAAGTCCCGGGAAGGTAAATGCCAAACCCAGAGCC ATGTGAGATTCTGACTTTGATCTTGGCTGTGGTTTAATAGCATCTCAGGATTATTTATCATATTCTCTTTTTTTCTTATTGCTTATAAGGTGTCAGTTAT TGTTTCCATTGTTGTTTACTTTAGTTCTGGAACTGAAACCGACAGGAAGAAACTTTGGAATAAGGAAGAGAAGATTGACCAAACCATCCAAATGCCAAAA A

refseq_HTR4.F1 (forward primer)
refseq_HTR4.R1 (reverse primer)
Single exon skipping

Frame 0

GAGSGWERSGSGGSRSSAQRLGVAARICQGTSL PRLWQRDPVGIEIQLTHAYFL*WTNLMLM*VLRR VSGQWRRWCCSRFSRRLS*WPSWGTCW*WWLCA GTGSSGK*KQIISLYLLLLRICWFRCW*CPLVP LSWFKTSGFMGRCFVLFGHLWTSCSQRHRFFTCA AFLWIGITPSAASLWSIGTR*PLCASH*CWEAA GSSPRLFLFSL*CKAGITLA*LI**KRGSSTRT LTLRTVSSWSTSPTPSPALWWPSTSHFSSWCWPI TASMSQLRSMPIRSRCYNGQEPPPRAGLSRQTS IALIA*GQRPKQPRPCASSWVASASAGHHSLSP ILWILS*TTLSLGRCGLLSSGSAISIPG*TLFST PS*ISLLDVPSSSSSAVMMSATEDLPFWARLSL VQPQPLMDPHMY*GQIFCLTETF*QGIGQSLPE RVHSQAH*ALDV*LQGSLSWGMQWSVVASGRVSV TRQQLLLWWLLSPVTLRPLGQ*PRRQPCLRKRA RS*AAACARLHPVFSSPEAFRPPVQEPGARWAF PLRFQQVALEGVRGHNGLLVHFLFPNTPSSQILC FCRVVSGVSDMFPSLTVSDLSHSRSKHDSLLRP ISVTDVSKYAFFAVWASRYQQNTPSLFLS*PEA SLLSCLLAWSRPSQPCSSYPLPLLRPVLCQTLTH FSPCVWVQTCILLVGPLVSPRRPLALPYVQCLS SSSGHAPFIFHGRHRVRDLSEENGLHSNSGGKL KTDSFLLVSWPLACLP*DARSPAGLERDVF*KAL GRASNIVGVY*ASGSFP*TCDIILKPFNLALLL PKEITVGSSHWGNSFSPAAPPRCRRAGSSSTGD AEAMLYLKPEDPNCRPGCAANKL*DLE*CSFISG PHFPHLCTQAWTRGPLSFLSVSKTTVCCYSRLL PREAMACLPFGFVSKERPSSFGPFRPLTFPALS FRLIWMSFIQS*VSGSCECPIAEVPGR*MPNPEP CEILTLILAVV**HLRIIYHILFFSYCL*GVSY CFHCCLL*FWN*NRQEETLE*GRED*PNHPNAK

Frame 1

AQAAAGSAAAAAAAEARLSGWGWPLESARAPRS LASGSGTLWALKSNSLMLISCNGQT*C*CEF*G GFRVSGEGGAAHVSLDGYPDGHLGEPAGDGGCVL GQAAQENKNKLFHCISCFCGSAGFGAGDALWCH *AGSRHLDLWGGVLSCSDISGRPAHNGIDFSPV LHFSG*VLRHLLPAFGL*EQDDPSAHRINAGRLL GHPHVYFFSPYNARLE*HWHN*FDRKEEVQPEL *LYVLCLHGQQALRHHLLCGGLLHPISPHGAGL LPHLCHS*GACPSDPDVTTGRSLLREQASVGRPA *HSSHEDRDQSSQDPVHHHGLLLPLLGTILCHQ YCGSFHRLHCPWAGVDCFPLARLYQFRVEPFSL RLLE*VF*TCLPHHPLL***ALPKTFHSGPDCPL FNHNH*WIHTCTKDRFSV*QRHFSKVLDKACQS GSILRHIEH*MFDCKEACLGGCSGVWWPVGESV SPASNFSFGGCSAQ*HLGPWDNDPEDSHASERGP GPKLLLVRDCTRYSLHLRLSVRQCRNPVLAGLF L*DSSRWRWRESGDIMASLFTFYFPTLPLPRFS AFAAWSLVSLTCSRHSLCLICLTHVLSTIACCVL SRSLMSPNTPSLLCGLPDINKILPLCSCLSQRR RSCLAYLLGHVRHSHVQAIPCLFFALFCARR*H TFLPVSGSKRASSWLAPLSVPDVHLLFLMSNVCP PLQDMLHLFSMEDTGSEICQRKMVSTATLEGS* RQIASSWCPGRWRVCPEMLALLLG*NGTSSKKP WAGLATS*VFIRLQDLSHELVISF*NPSIWLCCC QRR*LLEVHTGETHFPLQLPQGAGGQDPAALGM PRQCCT*SQRTQIAALAVLQTSCEILNSVLSSL DPTFHICALKLGLEDL*VFFLFLRQQYAAIAVCY LEKPWPACLLALFQRNDPPHLGLLDP*PFQLCP SD*SGCLLFKAESVALANAP*QKSREGKCQTQS HVRF*L*SWLWFNSISGLFIIFSFFLIAYKVSVI VSIVVYFSSGTETDRKKLWNKEEKIDQTIQMPK

Frame 2

RRQRLGAQRQRRQQKLGSAAGGGRSNLPGHLAP SPLAAGPCGH*NPTHSCLFPVMDKLDANVSSEE GFGSVEKVVLLTFLSTVILMAILGNLLVMVAVC WDRQLRKIKTNYFIVSLAFADLLVSVLVMPFGAI ELVQDIWIYGEVFCLVRTSLDVLLTTASIFHLC CISLDRYYAICCQPLVYRNKMTPLRIALMLGGC WVIPTFISFLPIMQGWNNIGIIDLIEKRKFNQNS NSTYCVFMVNKPYAITCSVVAFYIPFLLMVLAY YRIYVTAKEHAHQIQMLQRAGASSESRPQSADQ HSTHRMRTETKAAKTLCIIMGCFCLCWAPFFVTN IVDPFIDYTVPGQVWTAFLWLGYINSGLNPFLY AFLNKSFRRAFLIILCCDDERYRRPSILGQTVP CSTTTINGSTHVLRTDFLFDRDILARYWTKPARA GPFSGTLSIRCLTARKPVLGDAVECGGQWESQC HPPATSPLVAAQPSDT*APGTMTQKTAMPPKEG QVLSCCLCATAPGILFT*GFPSASAGTRCSLGFS SEIPAGGAGGSQGT*WPPCSLFISQHSLFPDSL LLPRGLWCL*HVPVTHCV*SVSLTF*AR*LAAS YLGH*CLQIRLLCCVGFQISTKYSLSVPVLARGV APVLLTCLVTSVTAMFKLSPASSSPCSVPDANT LFSLCLGPNVHPLGWPPCQSQTSTCSSLCPMSV LLFRTCSIYFPWKTQGQRSVRGKWSPQQLWREAE DR*LPPGVLAAGVSALRCSLSCWARTGRLLKSL GQG*QHRRCLLGFRIFPMNL*YHFETLQSGFVA AKGDNCWKFTLGKLIFPCSSPKVQEGRIQQHWGC RGNAVLEARGPKLPPWLCCKQAVRS*IVFFHLW TPLSTSVHSSLD*RTFKFSFCF*DNSMLL*PSA TSRSHGLPAFWLCFKGTTLLIWAF*TPDLSSFVL QINLDVFYSKLSQWLLRMPHSRSPGKVNAKPRA M*DSDFDLGCGLIASQDYLSYSLFFLLLIRCQL LFPLLFTLVLELKPTGRNFGIRKRRLTKPSKCQK