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Sequences

Sequence NM_025256

GCAAGCGGCGATGGCGGCGGCGGCGGGAGCTGCAGCGGCGGCGGCCGCCGAGGGGGAGGCCCCCGCTGAGATGGGGGCGCTGCTGCTGGAGAAGGAAACC AGAGGAGCCACCGAGAGAGTTCATGGCTCTTTGGGGGACACCCCTCGTAGTGAAGAAACCCTGCCCAAGGCCACCCCCGACTCCCTGGAGCCTGCTGGCC CCTCATCTCCAGCCTCTGTCACTGTCACTGTTGGTGATGAGGGGGCTGACACCCCTGTAGGGGCTACACCACTCATTGGGGATGAATCTGAGAATCTTGA GGGAGATGGGGACCTCCGTGGGGGCCGGATCCTGCTGGGCCATGCCACAAAGTCATTCCCCTCTTCCCCCAGCAAGGGGGGTTCCTGTCCTAGCCGGGCC AAGATGTCAATGACAGGGGCGGGAAAATCACCTCCATCTGTCCAGAGTTTGGCTATGAGGCTACTGAGTATGCCAGGAGCCCAGGGAGCTGCAGCAGCAG GGTCTGAACCCCCTCCAGCCACCACGAGCCCAGAGGGACAGCCCAAGGTCCACCGAGCCCGCAAAACCATGTCCAAACCAGGAAATGGACAGCCCCCGGT CCCTGAGAAGCGGCCCCCTGAAATACAGCATTTCCGCATGAGTGATGATGTCCACTCACTGGGAAAGGTGACCTCAGATCTGGCCAAAAGGAGGAAGCTG AACTCAGGAGGTGGCCTGTCAGAGGAGTTAGGTTCTGCCCGGCGTTCAGGAGAAGTGACCCTGACGAAAGGGGACCCCGGGTCCCTGGAGGAGTGGGAGA CGGTGGTGGGTGATGACTTCAGTCTCTACTATGATTCCTACTCTGTGGATGAGCGCGTGGACTCCGACAGCAAGTCTGAAGTTGAAGCTCTAACTGAACA ACTAAGTGAAGAGGAGGAGGAGGAAGAGGAGGAAGAAGAAGAAGAGGAAGAGGAGGAGGAAGAGGAAGAAGAAGAGGAAGATGAGGAGTCAGGGAATCAG TCAGATAGGAGTGGTTCCAGTGGCCGGCGCAAGGCCAAGAAGAAATGGCGAAAAGACAGCCCATGGGTGAAGCCGTCTCGGAAACGGCGCAAGCGGGAGC CTCCGCGGGCCAAGGAGCCACGAGGGGTGTCCAATGACACATCTTCGCTGGAGACAGAGCGAGGGTTTGAGGAGTTGCCCCTGTGCAGCTGCCGCATGGA GGCACCCAAGATTGACCGCATCAGCGAGAGGGCGGGGCACAAGTGCATGGCCACTGAGAGTGTGGACGGAGAGCTGTCAGGCTGCAATGCCGCCATCCTC AAGCGGGAGACCATGAGGCCATCCAGCCGTGTGGCCCTGATGGTGCTCTGTGAGACCCACCGCGCCCGCATGGTCAAACACCACTGCTGCCCGGGCTGCG GCTACTTCTGCACGGCGGGCACCTTCCTGGAGTGCCACCCTGACTTCCGTGTGGCCCACCGCTTCCACAAGGCCTGTGTGTCTCAGCTGAATGGGATGGT CTTCTGTCCCCACTGTGGGGAGGATGCTTCTGAAGCTCAAGAGGTGACCATCCCCCGGGGTGACGGGGTGACCCCACCGGCCGGCACTGCAGCTCCTGCA CCCCCACCCCTGTCCCAGGATGTCCCCGGGAGAGCAGACACTTCTCAGCCCAGTGCCCGGATGCGAGGGCATGGGGAACCCCGGCGCCCGCCCTGCGATC CCCTGGCTGACACCATTGACAGCTCAGGGCCCTCCCTGACCCTGCCCAATGGGGGCTGCCTTTCAGCCGTGGGGCTGCCACTGGGGCCAGGCCGGGAGGC CCTGGAAAAGGCCCTGGTCATCCAGGAGTCAGAGAGGCGGAAGAAGCTCCGTTTCCACCCTCGGCAGTTGTACCTGTCCGTGAAGCAGGGCGAGCTGCAG AAGGTGATCCTGATGCTGTTGGACAACCTGGACCCCAACTTCCAGAGCGACCAGCAGAGCAAGCGCACGCCCCTGCATGCAGCCGCCCAGAAGGGCTCCG TGGAGATCTGCCATGTGCTGCTGCAGGCTGGAGCCAACATAAATGCAGTGGACAAACAGCAGCGGACGCCACTGATGGAGGCCGTGGTGAACAACCACCT GGAGGTAGCCCGTTACATGGTGCAGCGTGGTGGCTGTGTCTATAGCAAGGAGGAGGACGGTTCCACCTGCCTCCACCACGCAGCCAAAATCGGGAACTTG GAGATGGTCAGCCTGCTGCTGAGCACAGGACAGGTGGACGTCAACGCCCAGGACAGTGGGGGGTGGACGCCCATCATCTGGGCTGCAGAGCACAAGCACA TCGAGGTGATCCGCATGCTACTGACGCGGGGCGCCGACGTCACCCTCACTGACAACGAGGAGAACATCTGCCTGCACTGGGCCTCCTTCACGGGCAGCGC CGCCATCGCCGAAGTCCTTCTGAATGCGCGCTGTGACCTCCATGCTGTCAACTACCATGGGGACACCCCCCTGCACATCGCAGCTCGGGAGAGCTACCAT GACTGCGTGCTGTTATTCCTGTCACGTGGGGCCAACCCTGAGCTGCGGAACAAAGAGGGGGACACAGCATGGGACCTGACTCCCGAGCGCTCCGACGTGT GGTTTGCGCTTCAACTCAACCGCAAGCTCCGACTTGGGGTGGGAAATCGGGCCATCCGCACAGAGAAGATCATCTGCCGGGACGTGGCTCGGGGCTATGA GAACGTGCCCATTCCCTGTGTCAACGGTGTGGATGGGGAGCCCTGCCCTGAGGATTACAAGTACATCTCAGAGAACTGCGAGACGTCCACCATGAACATC GATCGCAACATCACCCACCTGCAGCACTGCACGTGTGTGGACGACTGCTCTAGCTCCAACTGCCTGTGCGGCCAGCTCAGCATCCGGTGCTGGTATGACA AGGATGGGCGATTGCTCCAGGAATTTAACAAGATTGAGCCTCCGCTGATTTTCGAGTGTAACCAGGCGTGCTCATGCTGGAGAAACTGCAAGAACCGGGT CGTACAGAGTGGCATCAAGGTGCGGCTACAGCTCTACCGAACAGCCAAGATGGGCTGGGGGGTCCGCGCCCTGCAGACCATCCCACAGGGGACCTTCATC TGCGAGTATGTCGGGGAGCTGATCTCTGATGCTGAGGCTGATGTGAGAGAGGATGATTCTTACCTCTTCGACTTAGACAACAAGGATGGAGAGGTGTACT GCATAGATGCCCGTTACTATGGCAACATCAGCCGCTTCATCAACCACCTGTGTGACCCCAACATCATTCCCGTCCGGGTCTTCATGCTGCACCAAGACCT GCGATTTCCACGCATCGCCTTCTTCAGTTCCCGAGACATCCGGACTGGGGAGGAGCTAGGGTTTGACTATGGCGACCGCTTCTGGGACATCAAAAGCAAA TATTTCACCTGCCAATGTGGCTCTGAGAAGTGCAAGCACTCAGCCGAAGCCATTGCCCTGGAGCAGAGCCGTCTGGCCCGCCTGGACCCACACCCTGAGC TGCTGCCCGAGCTCGGCTCCCTGCCCCCTGTCAACACATGAGAACGGACCACACCCTCTCTCCCCAGCATGGATGGCCACAGCTCAGCCGCCTCCTCTGC CACCAGCTGCTCGCAGCCCATGCCTGGGGGTGCTGCCATCTTCTCTCCCCACCACCCTTTCACACATTCCTGACCAGAGATCCCAGCCAGGCCCTGGAGG TCTGACAGCCCCTCCCTCCCAGAGCTGGTTCCTCCCTGGGAGGGCAACTTCAGGGCTGGCCACCCCCCGTGTTCCCCATCCTCAGTTGAAGTTTGATGAA TTGAAGTCGGGCCTCTATGCCAACTGGTTCCTTTTGTTCTCAATAAATGTTGGGTTTGGTAATAAA

refseq_EHMT2.F1 (forward primer)
refseq_EHMT2.R1 (reverse primer)
Single exon skipping

Frame 0

ASGDGGGGGSCSGGGRRGGGPR*DGGAAAGEGN QRSHRESSWLFGGHPS**RNPAQGHPRLPGACWP LISSLCHCHCW**GG*HPCRGYTTHWG*I*ES* GRWGPPWGPDPAGPCHKVIPLFPQQGGFLS*PG QDVNDRGGKITSICPEFGYEATEYARSPGSCSSR V*TPSSHHEPRGTAQGPPSPQNHVQTRKWTAPG P*EAAP*NTAFPHE**CPLTGKGDLRSGQKEEA ELRRWPVRGVRFCPAFRRSDPDERGPRVPGGVGD GGG**LQSLL*FLLCG*ARGLRQQV*S*SSN*T TK*RGGGGRGGRRRRGRGGGRGRRRGR*GVRES VR*EWFQWPAQGQEEMAKRQPMGEAVSETAQAGA SAGQGATRGVQ*HIFAGDRARV*GVAPVQLPHG GTQD*PHQREGGAQVHGH*ECGRRAVRLQCRHP QAGDHEAIQPCGPDGAL*DPPRPHGQTPLLPGLR LLLHGGHLPGVPP*LPCGPPLPQGLCVSAEWDG LLSPLWGGCF*SSRGDHPPG*RGDPTGRHCSSC TPTPVPGCPRESRHFSAQCPDARAWGTPAPALRS PG*HH*QLRALPDPAQWGLPFSRGAATGARPGG PGKGPGHPGVREAEEAPFPPSAVVPVREAGRAA EGDPDAVGQPGPQLPERPAEQAHAPACSRPEGLR GDLPCAAAGWSQHKCSGQTAADATDGGRGEQPP GGSPLHGAAWWLCL*QGGGRFHLPPPRSQNREL GDGQPAAEHRTGGRQRPGQWGVDAHHLGCRAQAH RGDPHATDAGRRRHPH*QRGEHLPALGLLHGQR RHRRSPSECAL*PPCCQLPWGHPPAHRSSGELP *LRAVIPVTWGQP*AAEQRGGHSMGPDSRALRRV VCASTQPQAPTWGGKSGHPHREDHLPGRGSGL* ERAHSLCQRCGWGALP*GLQVHLRELRDVHHEH RSQHHPPAALHVCGRLL*LQLPVRPAQHPVLV*Q GWAIAPGI*QD*ASADFRV*PGVLMLEKLQEPG RTEWHQGAATALPNSQDGLGGPRPADHPTGDLH LRVCRGADL*C*G*CERG*FLPLRLRQQGWRGVL HRCPLLWQHQPLHQPPV*PQHHSRPGLHAAPRP AISTHRLLQFPRHPDWGGARV*LWRPLLGHQKQ IFHLPMWL*EVQALSRSHCPGAEPSGPPGPTP*A AARARLPAPCQHMRTDHTLSPQHGWPQLSRLLC HQLLAAHAWGCCHLLSPPPFHTFLTRDPSQALE V*QPLPPRAGSSLGGQLQGWPPPVFPILS*SLMN *SRASMPTGSFCSQ*MLGLVI

Frame 1

QAAMAAAAGAAAAAAAEGEAPAEMGALLLEKET RGATERVHGSLGDTPRSEETLPKATPDSLEPAG PSSPASVTVTVGDEGADTPVGATPLIGDESENLE GDGDLRGGRILLGHATKSFPSSPSKGGSCPSRA KMSMTGAGKSPPSVQSLAMRLLSMPGAQGAAAA GSEPPPATTSPEGQPKVHRARKTMSKPGNGQPPV PEKRPPEIQHFRMSDDVHSLGKVTSDLAKRRKL NSGGGLSEELGSARRSGEVTLTKGDPGSLEEWE TVVGDDFSLYYDSYSVDERVDSDSKSEVEALTEQ LSEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQ SDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKRE PPRAKEPRGVSNDTSSLETERGFEELPLCSCRME APKIDRISERAGHKCMATESVDGELSGCNAAIL KRETMRPSSRVALMVLCETHRARMVKHHCCPGC GYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMV FCPHCGEDASEAQEVTIPRGDGVTPPAGTAAPA PPPLSQDVPGRADTSQPSARMRGHGEPRRPPCD PLADTIDSSGPSLTLPNGGCLSAVGLPLGPGREA LEKALVIQESERRKKLRFHPRQLYLSVKQGELQ KVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGS VEICHVLLQAGANINAVDKQQRTPLMEAVVNNHL EVARYMVQRGGCVYSKEEDGSTCLHHAAKIGNL EMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKH IEVIRMLLTRGADVTLTDNEENICLHWASFTGSA AIAEVLLNARCDLHAVNYHGDTPLHIAARESYH DCVLLFLSRGANPELRNKEGDTAWDLTPERSDV WFALQLNRKLRLGVGNRAIRTEKIICRDVARGYE NVPIPCVNGVDGEPCPEDYKYISENCETSTMNI DRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYD KDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRV VQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFI CEYVGELISDAEADVREDDSYLFDLDNKDGEVY CIDARYYGNISRFINHLCDPNIIPVRVFMLHQDL RFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSK YFTCQCGSEKCKHSAEAIALEQSRLARLDPHPE LLPELGSLPPVNT*ERTTPSLPSMDGHSSAASSA TSCSQPMPGGAAIFSPHHPFTHS*PEIPARPWR SDSPSLPELVPPWEGNFRAGHPPCSPSSVEV** IEVGPLCQLVPFVLNKCWVW**

Frame 2

KRRWRRRRELQRRRPPRGRPPLRWGRCCWRRKP EEPPREFMALWGTPLVVKKPCPRPPPTPWSLLA PHLQPLSLSLLVMRGLTPL*GLHHSLGMNLRIL REMGTSVGAGSCWAMPQSHSPLPPARGVPVLAGP RCQ*QGRENHLHLSRVWL*GY*VCQEPRELQQQ GLNPLQPPRAQRDSPRSTEPAKPCPNQEMDSPR SLRSGPLKYSISA*VMMSTHWER*PQIWPKGGS* TQEVACQRS*VLPGVQEK*P*RKGTPGPWRSGR RWWVMTSVSTMIPTLWMSAWTPTASLKLKL*LN N*VKRRRRKRRKKKKRKRRRKRKKKRKMRSQGIS QIGVVPVAGARPRRNGEKTAHG*SRLGNGASGS LRGPRSHEGCPMTHLRWRQSEGLRSCPCAAAAW RHPRLTASARGRGTSAWPLRVWTESCQAAMPPSS SGRP*GHPAVWP*WCSVRPTAPAWSNTTAARAA ATSARRAPSWSATLTSVWPTASTRPVCLS*MGW SSVPTVGRMLLKLKR*PSPGVTG*PHRPALQLLH PHPCPRMSPGEQTLLSPVPGCEGMGNPGARPAI PWLTPLTAQGPP*PCPMGAAFQPWGCHWGQAGR PWKRPWSSRSQRGGRSSVSTLGSCTCP*SRASCR R*S*CCWTTWTPTSRATSRASARPCMQPPRRAP WRSAMCCCRLEPT*MQWTNSSGRH*WRPW*TTT WR*PVTWCSVVAVSIARRRTVPPASTTQPKSGTW RWSACC*AQDRWTSTPRTVGGGRPSSGLQSTST SR*SACY*RGAPTSPSLTTRRTSACTGPPSRAA PPSPKSF*MRAVTSMLSTTMGTPPCTSQLGRATM TACCYSCHVGPTLSCGTKRGTQHGT*LPSAPTC GLRFNSTASSDLGWEIGPSAQRRSSAGTWLGAM RTCPFPVSTVWMGSPALRITSTSQRTARRPP*TS IATSPTCSTARVWTTALAPTACAASSASGAGMT RMGDCSRNLTRLSLR*FSSVTRRAHAGETARTG SYRVASRCGYSSTEQPRWAGGSAPCRPSHRGPSS ASMSGS*SLMLRLM*ERMILTSST*TTRMERCT A*MPVTMATSAASSTTCVTPTSFPSGSSCCTKT CDFHASPSSVPETSGLGRS*GLTMATASGTSKAN ISPANVALRSASTQPKPLPWSRAVWPAWTHTLS CCPSSAPCPLSTHENGPHPLSPAWMATAQPPPL PPAARSPCLGVLPSSLPTTLSHIPDQRSQPGPGG LTAPPSQSWFLPGRATSGLATPRVPHPQLKFDE LKSGLYANWFLLFSINVGFGNK

Sequence NM_006709

GCAAGCGGCGATGGCGGCGGCGGCGGGAGCTGCAGCGGCGGCGGCCGCCGAGGGGGAGGCCCCCGCTGAGATGGGGGCGCTGCTGCTGGAGAAGGAAACC AGAGGAGCCACCGAGAGAGTTCATGGCTCTTTGGGGGACACCCCTCGTAGTGAAGAAACCCTGCCCAAGGCCACCCCCGACTCCCTGGAGCCTGCTGGCC CCTCATCTCCAGCCTCTGTCACTGTCACTGTTGGTGATGAGGGGGCTGACACCCCTGTAGGGGCTACACCACTCATTGGGGATGAATCTGAGAATCTTGA GGGAGATGGGGACCTCCGTGGGGGCCGGATCCTGCTGGGCCATGCCACAAAGTCATTCCCCTCTTCCCCCAGCAAGGGGGGTTCCTGTCCTAGCCGGGCC AAGATGTCAATGACAGGGGCGGGAAAATCACCTCCATCTGTCCAGAGTTTGGCTATGAGGCTACTGAGTATGCCAGGAGCCCAGGGAGCTGCAGCAGCAG GGTCTGAACCCCCTCCAGCCACCACGAGCCCAGAGGGACAGCCCAAGGTCCACCGAGCCCGCAAAACCATGTCCAAACCAGGAAATGGACAGCCCCCGGT CCCTGAGAAGCGGCCCCCTGAAATACAGCATTTCCGCATGAGTGATGATGTCCACTCACTGGGAAAGGTGACCTCAGATCTGGCCAAAAGGAGGAAGCTG AACTCAGGAGGTGGCCTGTCAGAGGAGTTAGGTTCTGCCCGGCGTTCAGGAGAAGTGACCCTGACGAAAGGGGACCCCGGGTCCCTGGAGGAGTGGGAGA CGGTGGTGGGTGATGACTTCAGTCTCTACTATGATTCCTACTCTGTGGATGAGCGCGTGGACTCCGACAGCAAGTCTGAAGTTGAAGCTCTAACTGAACA ACTAAGTGAAGAGGAGGAGGAGGAAGAGGAGGAAGAAGAAGAAGAGGAAGAGGAGGAGGAAGAGGAAGAAGAAGAGGAAGATGAGGAGTCAGGGAATCAG TCAGATAGGAGTGGTTCCAGTGGCCGGCGCAAGGCCAAGAAGAAATGGCGAAAAGACAGCCCATGGGTGAAGCCGTCTCGGAAACGGCGCAAGCGGGAGC CTCCGCGGGCCAAGGAGCCACGAGGAGTGAATGGTGTGGGCTCCTCAGGCCCCAGTGAGTACATGGAGGTCCCTCTGGGGTCCCTGGAGCTGCCCAGCGA GGGGACCCTCTCCCCCAACCACGCTGGGGTGTCCAATGACACATCTTCGCTGGAGACAGAGCGAGGGTTTGAGGAGTTGCCCCTGTGCAGCTGCCGCATG GAGGCACCCAAGATTGACCGCATCAGCGAGAGGGCGGGGCACAAGTGCATGGCCACTGAGAGTGTGGACGGAGAGCTGTCAGGCTGCAATGCCGCCATCC TCAAGCGGGAGACCATGAGGCCATCCAGCCGTGTGGCCCTGATGGTGCTCTGTGAGACCCACCGCGCCCGCATGGTCAAACACCACTGCTGCCCGGGCTG CGGCTACTTCTGCACGGCGGGCACCTTCCTGGAGTGCCACCCTGACTTCCGTGTGGCCCACCGCTTCCACAAGGCCTGTGTGTCTCAGCTGAATGGGATG GTCTTCTGTCCCCACTGTGGGGAGGATGCTTCTGAAGCTCAAGAGGTGACCATCCCCCGGGGTGACGGGGTGACCCCACCGGCCGGCACTGCAGCTCCTG CACCCCCACCCCTGTCCCAGGATGTCCCCGGGAGAGCAGACACTTCTCAGCCCAGTGCCCGGATGCGAGGGCATGGGGAACCCCGGCGCCCGCCCTGCGA TCCCCTGGCTGACACCATTGACAGCTCAGGGCCCTCCCTGACCCTGCCCAATGGGGGCTGCCTTTCAGCCGTGGGGCTGCCACTGGGGCCAGGCCGGGAG GCCCTGGAAAAGGCCCTGGTCATCCAGGAGTCAGAGAGGCGGAAGAAGCTCCGTTTCCACCCTCGGCAGTTGTACCTGTCCGTGAAGCAGGGCGAGCTGC AGAAGGTGATCCTGATGCTGTTGGACAACCTGGACCCCAACTTCCAGAGCGACCAGCAGAGCAAGCGCACGCCCCTGCATGCAGCCGCCCAGAAGGGCTC CGTGGAGATCTGCCATGTGCTGCTGCAGGCTGGAGCCAACATAAATGCAGTGGACAAACAGCAGCGGACGCCACTGATGGAGGCCGTGGTGAACAACCAC CTGGAGGTAGCCCGTTACATGGTGCAGCGTGGTGGCTGTGTCTATAGCAAGGAGGAGGACGGTTCCACCTGCCTCCACCACGCAGCCAAAATCGGGAACT TGGAGATGGTCAGCCTGCTGCTGAGCACAGGACAGGTGGACGTCAACGCCCAGGACAGTGGGGGGTGGACGCCCATCATCTGGGCTGCAGAGCACAAGCA CATCGAGGTGATCCGCATGCTACTGACGCGGGGCGCCGACGTCACCCTCACTGACAACGAGGAGAACATCTGCCTGCACTGGGCCTCCTTCACGGGCAGC GCCGCCATCGCCGAAGTCCTTCTGAATGCGCGCTGTGACCTCCATGCTGTCAACTACCATGGGGACACCCCCCTGCACATCGCAGCTCGGGAGAGCTACC ATGACTGCGTGCTGTTATTCCTGTCACGTGGGGCCAACCCTGAGCTGCGGAACAAAGAGGGGGACACAGCATGGGACCTGACTCCCGAGCGCTCCGACGT GTGGTTTGCGCTTCAACTCAACCGCAAGCTCCGACTTGGGGTGGGAAATCGGGCCATCCGCACAGAGAAGATCATCTGCCGGGACGTGGCTCGGGGCTAT GAGAACGTGCCCATTCCCTGTGTCAACGGTGTGGATGGGGAGCCCTGCCCTGAGGATTACAAGTACATCTCAGAGAACTGCGAGACGTCCACCATGAACA TCGATCGCAACATCACCCACCTGCAGCACTGCACGTGTGTGGACGACTGCTCTAGCTCCAACTGCCTGTGCGGCCAGCTCAGCATCCGGTGCTGGTATGA CAAGGATGGGCGATTGCTCCAGGAATTTAACAAGATTGAGCCTCCGCTGATTTTCGAGTGTAACCAGGCGTGCTCATGCTGGAGAAACTGCAAGAACCGG GTCGTACAGAGTGGCATCAAGGTGCGGCTACAGCTCTACCGAACAGCCAAGATGGGCTGGGGGGTCCGCGCCCTGCAGACCATCCCACAGGGGACCTTCA TCTGCGAGTATGTCGGGGAGCTGATCTCTGATGCTGAGGCTGATGTGAGAGAGGATGATTCTTACCTCTTCGACTTAGACAACAAGGATGGAGAGGTGTA CTGCATAGATGCCCGTTACTATGGCAACATCAGCCGCTTCATCAACCACCTGTGTGACCCCAACATCATTCCCGTCCGGGTCTTCATGCTGCACCAAGAC CTGCGATTTCCACGCATCGCCTTCTTCAGTTCCCGAGACATCCGGACTGGGGAGGAGCTAGGGTTTGACTATGGCGACCGCTTCTGGGACATCAAAAGCA AATATTTCACCTGCCAATGTGGCTCTGAGAAGTGCAAGCACTCAGCCGAAGCCATTGCCCTGGAGCAGAGCCGTCTGGCCCGCCTGGACCCACACCCTGA GCTGCTGCCCGAGCTCGGCTCCCTGCCCCCTGTCAACACATGAGAACGGACCACACCCTCTCTCCCCAGCATGGATGGCCACAGCTCAGCCGCCTCCTCT GCCACCAGCTGCTCGCAGCCCATGCCTGGGGGTGCTGCCATCTTCTCTCCCCACCACCCTTTCACACATTCCTGACCAGAGATCCCAGCCAGGCCCTGGA GGTCTGACAGCCCCTCCCTCCCAGAGCTGGTTCCTCCCTGGGAGGGCAACTTCAGGGCTGGCCACCCCCCGTGTTCCCCATCCTCAGTTGAAGTTTGATG AATTGAAGTCGGGCCTCTATGCCAACTGGTTCCTTTTGTTCTCAATAAATGTTGGGTTTGGTAATAAA

refseq_EHMT2.F1 (forward primer)
refseq_EHMT2.R1 (reverse primer)
Single exon skipping

Frame 0

ASGDGGGGGSCSGGGRRGGGPR*DGGAAAGEGN QRSHRESSWLFGGHPS**RNPAQGHPRLPGACWP LISSLCHCHCW**GG*HPCRGYTTHWG*I*ES* GRWGPPWGPDPAGPCHKVIPLFPQQGGFLS*PG QDVNDRGGKITSICPEFGYEATEYARSPGSCSSR V*TPSSHHEPRGTAQGPPSPQNHVQTRKWTAPG P*EAAP*NTAFPHE**CPLTGKGDLRSGQKEEA ELRRWPVRGVRFCPAFRRSDPDERGPRVPGGVGD GGG**LQSLL*FLLCG*ARGLRQQV*S*SSN*T TK*RGGGGRGGRRRRGRGGGRGRRRGR*GVRES VR*EWFQWPAQGQEEMAKRQPMGEAVSETAQAGA SAGQGATRSEWCGLLRPQ*VHGGPSGVPGAAQR GDPLPQPRWGVQ*HIFAGDRARV*GVAPVQLPH GGTQD*PHQREGGAQVHGH*ECGRRAVRLQCRHP QAGDHEAIQPCGPDGAL*DPPRPHGQTPLLPGL RLLLHGGHLPGVPP*LPCGPPLPQGLCVSAEWD GLLSPLWGGCF*SSRGDHPPG*RGDPTGRHCSSC TPTPVPGCPRESRHFSAQCPDARAWGTPAPALR SPG*HH*QLRALPDPAQWGLPFSRGAATGARPG GPGKGPGHPGVREAEEAPFPPSAVVPVREAGRAA EGDPDAVGQPGPQLPERPAEQAHAPACSRPEGL RGDLPCAAAGWSQHKCSGQTAADATDGGRGEQP PGGSPLHGAAWWLCL*QGGGRFHLPPPRSQNREL GDGQPAAEHRTGGRQRPGQWGVDAHHLGCRAQA HRGDPHATDAGRRRHPH*QRGEHLPALGLLHGQ RRHRRSPSECAL*PPCCQLPWGHPPAHRSSGELP *LRAVIPVTWGQP*AAEQRGGHSMGPDSRALRR VVCASTQPQAPTWGGKSGHPHREDHLPGRGSGL *ERAHSLCQRCGWGALP*GLQVHLRELRDVHHEH RSQHHPPAALHVCGRLL*LQLPVRPAQHPVLV* QGWAIAPGI*QD*ASADFRV*PGVLMLEKLQEP GRTEWHQGAATALPNSQDGLGGPRPADHPTGDLH LRVCRGADL*C*G*CERG*FLPLRLRQQGWRGV LHRCPLLWQHQPLHQPPV*PQHHSRPGLHAAPR PAISTHRLLQFPRHPDWGGARV*LWRPLLGHQKQ IFHLPMWL*EVQALSRSHCPGAEPSGPPGPTP* AAARARLPAPCQHMRTDHTLSPQHGWPQLSRLL CHQLLAAHAWGCCHLLSPPPFHTFLTRDPSQALE V*QPLPPRAGSSLGGQLQGWPPPVFPILS*SLM N*SRASMPTGSFCSQ*MLGLVI

Frame 1

QAAMAAAAGAAAAAAAEGEAPAEMGALLLEKET RGATERVHGSLGDTPRSEETLPKATPDSLEPAG PSSPASVTVTVGDEGADTPVGATPLIGDESENLE GDGDLRGGRILLGHATKSFPSSPSKGGSCPSRA KMSMTGAGKSPPSVQSLAMRLLSMPGAQGAAAA GSEPPPATTSPEGQPKVHRARKTMSKPGNGQPPV PEKRPPEIQHFRMSDDVHSLGKVTSDLAKRRKL NSGGGLSEELGSARRSGEVTLTKGDPGSLEEWE TVVGDDFSLYYDSYSVDERVDSDSKSEVEALTEQ LSEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQ SDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKRE PPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSE GTLSPNHAGVSNDTSSLETERGFEELPLCSCRM EAPKIDRISERAGHKCMATESVDGELSGCNAAI LKRETMRPSSRVALMVLCETHRARMVKHHCCPGC GYFCTAGTFLECHPDFRVAHRFHKACVSQLNGM VFCPHCGEDASEAQEVTIPRGDGVTPPAGTAAP APPPLSQDVPGRADTSQPSARMRGHGEPRRPPCD PLADTIDSSGPSLTLPNGGCLSAVGLPLGPGRE ALEKALVIQESERRKKLRFHPRQLYLSVKQGEL QKVILMLLDNLDPNFQSDQQSKRTPLHAAAQKGS VEICHVLLQAGANINAVDKQQRTPLMEAVVNNH LEVARYMVQRGGCVYSKEEDGSTCLHHAAKIGN LEMVSLLLSTGQVDVNAQDSGGWTPIIWAAEHKH IEVIRMLLTRGADVTLTDNEENICLHWASFTGS AAIAEVLLNARCDLHAVNYHGDTPLHIAARESY HDCVLLFLSRGANPELRNKEGDTAWDLTPERSDV WFALQLNRKLRLGVGNRAIRTEKIICRDVARGY ENVPIPCVNGVDGEPCPEDYKYISENCETSTMN IDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYD KDGRLLQEFNKIEPPLIFECNQACSCWRNCKNR VVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTF ICEYVGELISDAEADVREDDSYLFDLDNKDGEVY CIDARYYGNISRFINHLCDPNIIPVRVFMLHQD LRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKS KYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPE LLPELGSLPPVNT*ERTTPSLPSMDGHSSAASS ATSCSQPMPGGAAIFSPHHPFTHS*PEIPARPW RSDSPSLPELVPPWEGNFRAGHPPCSPSSVEV** IEVGPLCQLVPFVLNKCWVW**

Frame 2

KRRWRRRRELQRRRPPRGRPPLRWGRCCWRRKP EEPPREFMALWGTPLVVKKPCPRPPPTPWSLLA PHLQPLSLSLLVMRGLTPL*GLHHSLGMNLRIL REMGTSVGAGSCWAMPQSHSPLPPARGVPVLAGP RCQ*QGRENHLHLSRVWL*GY*VCQEPRELQQQ GLNPLQPPRAQRDSPRSTEPAKPCPNQEMDSPR SLRSGPLKYSISA*VMMSTHWER*PQIWPKGGS* TQEVACQRS*VLPGVQEK*P*RKGTPGPWRSGR RWWVMTSVSTMIPTLWMSAWTPTASLKLKL*LN N*VKRRRRKRRKKKKRKRRRKRKKKRKMRSQGIS QIGVVPVAGARPRRNGEKTAHG*SRLGNGASGS LRGPRSHEE*MVWAPQAPVSTWRSLWGPWSCPA RGPSPPTTLGCPMTHLRWRQSEGLRSCPCAAAAW RHPRLTASARGRGTSAWPLRVWTESCQAAMPPS SSGRP*GHPAVWP*WCSVRPTAPAWSNTTAARA AATSARRAPSWSATLTSVWPTASTRPVCLS*MGW SSVPTVGRMLLKLKR*PSPGVTG*PHRPALQLL HPHPCPRMSPGEQTLLSPVPGCEGMGNPGARPA IPWLTPLTAQGPP*PCPMGAAFQPWGCHWGQAGR PWKRPWSSRSQRGGRSSVSTLGSCTCP*SRASC RR*S*CCWTTWTPTSRATSRASARPCMQPPRRA PWRSAMCCCRLEPT*MQWTNSSGRH*WRPW*TTT WR*PVTWCSVVAVSIARRRTVPPASTTQPKSGT WRWSACC*AQDRWTSTPRTVGGGRPSSGLQSTS TSR*SACY*RGAPTSPSLTTRRTSACTGPPSRAA PPSPKSF*MRAVTSMLSTTMGTPPCTSQLGRAT MTACCYSCHVGPTLSCGTKRGTQHGT*LPSAPT CGLRFNSTASSDLGWEIGPSAQRRSSAGTWLGAM RTCPFPVSTVWMGSPALRITSTSQRTARRPP*T SIATSPTCSTARVWTTALAPTACAASSASGAGM TRMGDCSRNLTRLSLR*FSSVTRRAHAGETARTG SYRVASRCGYSSTEQPRWAGGSAPCRPSHRGPS SASMSGS*SLMLRLM*ERMILTSST*TTRMERC TA*MPVTMATSAASSTTCVTPTSFPSGSSCCTKT CDFHASPSSVPETSGLGRS*GLTMATASGTSKA NISPANVALRSASTQPKPLPWSRAVWPAWTHTL SCCPSSAPCPLSTHENGPHPLSPAWMATAQPPPL PPAARSPCLGVLPSSLPTTLSHIPDQRSQPGPG GLTAPPSQSWFLPGRATSGLATPRVPHPQLKFD ELKSGLYANWFLLFSINVGFGNK