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Sequences

Sequence NM_022664

ACAACCGTAACAGCCACCAGACAAGCTTCAGTGGCCGGCCCTTCACATCCAGACTTGCCTGAGAGGACCCACCTCTGAGTGTCCAGTGGTCAGTTGCCCC
AGGATGGGGACCACAGCCAGAGCAGCCTTGGTCTTGACCTATTTGGCTGTTGCTTCTGCTGCCTCTGAGGGAGGCTTCACGGCTACAGGACAGAGGCAGC
TGAGGCCAGAGCACTTTCAAGAAGTTGGCTACGCAGCTCCCCCCTCCCCACCCCTATCCCGAAGCCTCCCCATGGATCACCCTGACTCCTCTCAGCATGG
CCCTCCCTTTGAGGGACAGAGTCAAGTGCAGCCCCCTCCCTCTCAGGAGGCCACCCCTCTCCAACAGGAAAAGCTGCTACCTGCCCAACTCCCTGCTGAA
AAGGAAGTGGGTCCCCCTCTCCCTCAGGAAGCTGTCCCCCTCCAAAAAGAGCTGCCCTCTCTCCAGCACCCCAATGAACAGAAGGAAGGAACGCCAGCTC
CATTTGGGGACCAGAGCCATCCAGAACCTGAGTCCTGGAATGCAGCCCAGCACTGCCAACAGGACCGGTCCCAAGGGGGCTGGGGCCACCGGCTGGATGG
CTTCCCCCCTGGGCGGCCTTCTCCAGACAATCTGAACCAAATCTGCCTTCCTAACCGTCAGCATGTGGTATATGGTCCCTGGAACCTACCACAGTCCAGC
TACTCCCACCTCACTCGCCAGGGTGAGACCCTCAATTTCCTGGAGATTGGATATTCCCGCTGCTGCCACTGCCGCAGCCACACAAACCGCCTAGAGTGTG
CCAAACTTGTGTGGGAGGATACCCTTGACAAATACTGTGACCGGGAGTATGCTGTGAAGACCCACCACCACTTGTGTTGCCGCCACCCTCCCAGCCCTAC
TCGGGATGAGTGCTTTGCCCGTCGGGCTCCTTACCCCAACTATGACCGGGACATCTTGACCATTGACATCGGTCGAGTCACCCCCAACCTCATGGGCCAC
CTCTGTGGAAACCAAAGAGTTCTCACCAAGCATAAACATATTCCTGGGCTGATCCACAACATGACTGCCCGCTGCTGTGACCTGCCATTTCCAGAACAGG
CCTGCTGTGCAGAGGAGGAGAAATTAACCTTCATCAATGATCTGTGTGGTCCCCGACGTAACATCTGGCGAGACCCTGCCCTCTGCTGTTACCTGAGTCC
TGGGGATGAACAGGTCAACTGCTTCAACATCAATTATCTGAGGAACGTGGCTCTAGTGTCTGGAGACACTGAGAACGCCAAGGGCCAGGGGGAGCAGGGC
TCAACTGGAGGAACAAATATCAGCTCCACCTCTGAGCCCAAGGAAGAATGAGTCACCCCAGAGCCCTAGAGGGTCAGATGGGGGGAACCCCACCCTGCCC
CACCCATCTGAACACTCATTACACTAAACACCTCTTGGATTTGG
  • refseq_ECM1.F2 (forward primer)
  • refseq_ECM1.R2 (reverse primer)
  • Single exon skipping

Frame 0

TTVTATRQASVAGPSHPDLPERTHL*VSSGQLP
QDGDHSQSSLGLDLFGCCFCCL*GRLHGYRTEAA
EARALSRSWLRSSPLPTPIPKPPHGSP*LLSAW
PSL*GTESSAAPSLSGGHPSPTGKAATCPTPC*
KGSGSPSPSGSCPPPKRAALSPAPQ*TEGRNASS
IWGPEPSRT*VLECSPALPTGPVPRGLGPPAGW
LPPWAAFSRQSEPNLPS*PSACGIWSLEPTTVQ
LLPPHSPG*DPQFPGDWIFPLLPLPQPHKPPRVC
QTCVGGYP*QIL*PGVCCEDPPPLVLPPPSQPY
SG*VLCPSGSLPQL*PGHLDH*HRSSHPQPHGP
PLWKPKSSHQA*TYSWADPQHDCPLL*PAISRTG
LLCRGGEINLHQ*SVWSPT*HLARPCPLLLPES
WG*TGQLLQHQLSEERGSSVWRH*ERQGPGGAG
LNWRNKYQLHL*AQGRMSHPRALEGQMGGTPPCP
THLNTHYTKHLLDL

Frame 1

QP*QPPDKLQWPALHIQTCLRGPTSECPVVSCP
RMGTTARAALVLTYLAVASAASEGGFTATGQRQ
LRPEHFQEVGYAAPPSPPLSRSLPMDHPDSSQHG
PPFEGQSQVQPPPSQEATPLQQEKLLPAQLPAE
KEVGPPLPQEAVPLQKELPSLQHPNEQKEGTPA
PFGDQSHPEPESWNAAQHCQQDRSQGGWGHRLDG
FPPGRPSPDNLNQICLPNRQHVVYGPWNLPQSS
YSHLTRQGETLNFLEIGYSRCCHCRSHTNRLEC
AKLVWEDTLDKYCDREYAVKTHHHLCCRHPPSPT
RDECFARRAPYPNYDRDILTIDIGRVTPNLMGH
LCGNQRVLTKHKHIPGLIHNMTARCCDLPFPEQ
ACCAEEEKLTFINDLCGPRRNIWRDPALCCYLSP
GDEQVNCFNINYLRNVALVSGDTENAKGQGEQG
STGGTNISSTSEPKEE*VTPEP*RVRWGEPHPA
PPI*TLITLNTSWIW

Frame 2

NRNSHQTSFSGRPFTSRLA*EDPPLSVQWSVAP
GWGPQPEQPWS*PIWLLLLLPLREASRLQDRGS
*GQSTFKKLATQLPPPHPYPEASPWITLTPLSM
ALPLRDRVKCSPLPLRRPPLSNRKSCYLPNSLLK
RKWVPLSLRKLSPSKKSCPLSSTPMNRRKERQL
HLGTRAIQNLSPGMQPSTANRTGPKGAGATGWM
ASPLGGLLQTI*TKSAFLTVSMWYMVPGTYHSPA
TPTSLARVRPSISWRLDIPAAATAAATQTA*SV
PNLCGRIPLTNTVTGSML*RPTTTCVAATLPAL
LGMSALPVGLLTPTMTGTS*PLTSVESPPTSWAT
SVETKEFSPSINIFLG*STT*LPAAVTCHFQNR
PAVQRRRN*PSSMICVVPDVTSGETLPSAVT*V
LGMNRSTASTSII*GTWL*CLETLRTPRARGSRA
QLEEQISAPPLSPRKNESPQSPRGSDGGNPTLP
HPSEHSLH*TPLGF

Sequence NM_004425

ACAACCGTAACAGCCACCAGACAAGCTTCAGTGGCCGGCCCTTCACATCCAGACTTGCCTGAGAGGACCCACCTCTGAGTGTCCAGTGGTCAGTTGCCCC
AGGATGGGGACCACAGCCAGAGCAGCCTTGGTCTTGACCTATTTGGCTGTTGCTTCTGCTGCCTCTGAGGGAGGCTTCACGGCTACAGGACAGAGGCAGC
TGAGGCCAGAGCACTTTCAAGAAGTTGGCTACGCAGCTCCCCCCTCCCCACCCCTATCCCGAAGCCTCCCCATGGATCACCCTGACTCCTCTCAGCATGG
CCCTCCCTTTGAGGGACAGAGTCAAGTGCAGCCCCCTCCCTCTCAGGAGGCCACCCCTCTCCAACAGGAAAAGCTGCTACCTGCCCAACTCCCTGCTGAA
AAGGAAGTGGGTCCCCCTCTCCCTCAGGAAGCTGTCCCCCTCCAAAAAGAGCTGCCCTCTCTCCAGCACCCCAATGAACAGAAGGAAGGAACGCCAGCTC
CATTTGGGGACCAGAGCCATCCAGAACCTGAGTCCTGGAATGCAGCCCAGCACTGCCAACAGGACCGGTCCCAAGGGGGCTGGGGCCACCGGCTGGATGG
CTTCCCCCCTGGGCGGCCTTCTCCAGACAATCTGAACCAAATCTGCCTTCCTAACCGTCAGCATGTGGTATATGGTCCCTGGAACCTACCACAGTCCAGC
TACTCCCACCTCACTCGCCAGGGTGAGACCCTCAATTTCCTGGAGATTGGATATTCCCGCTGCTGCCACTGCCGCAGCCACACAAACCGCCTAGAGTGTG
CCAAACTTGTGTGGGAGGAAGCAATGAGCCGATTCTGTGAGGCCGAGTTCTCGGTCAAGACCCGACCCCACTGGTGCTGCACGCGGCAGGGGGAGGCTCG
GTTCTCCTGCTTCCAGGAGGAAGCTCCCCAGCCACACTACCAGCTCCGGGCCTGCCCCAGCCATCAGCCTGATATTTCCTCGGGTCTTGAGCTGCCTTTC
CCTCCTGGGGTGCCCACATTGGACAATATCAAGAACATCTGCCACCTGAGGCGCTTCCGCTCTGTGCCACGCAACCTGCCAGCTACTGACCCCCTACAAA
GGGAGCTGCTGGCACTGATCCAGCTGGAGAGGGAGTTCCAGCGCTGCTGCCGCCAGGGGAACAATCACACCTGTACATGGAAGGCCTGGGAGGATACCCT
TGACAAATACTGTGACCGGGAGTATGCTGTGAAGACCCACCACCACTTGTGTTGCCGCCACCCTCCCAGCCCTACTCGGGATGAGTGCTTTGCCCGTCGG
GCTCCTTACCCCAACTATGACCGGGACATCTTGACCATTGACATCGGTCGAGTCACCCCCAACCTCATGGGCCACCTCTGTGGAAACCAAAGAGTTCTCA
CCAAGCATAAACATATTCCTGGGCTGATCCACAACATGACTGCCCGCTGCTGTGACCTGCCATTTCCAGAACAGGCCTGCTGTGCAGAGGAGGAGAAATT
AACCTTCATCAATGATCTGTGTGGTCCCCGACGTAACATCTGGCGAGACCCTGCCCTCTGCTGTTACCTGAGTCCTGGGGATGAACAGGTCAACTGCTTC
AACATCAATTATCTGAGGAACGTGGCTCTAGTGTCTGGAGACACTGAGAACGCCAAGGGCCAGGGGGAGCAGGGCTCAACTGGAGGAACAAATATCAGCT
CCACCTCTGAGCCCAAGGAAGAATGAGTCACCCCAGAGCCCTAGAGGGTCAGATGGGGGGAACCCCACCCTGCCCCACCCATCTGAACACTCATTACACT
AAACACCTCTTGGATTTGG
  • refseq_ECM1.F2 (forward primer)
  • refseq_ECM1.R2 (reverse primer)
  • Single exon skipping

Frame 0

TTVTATRQASVAGPSHPDLPERTHL*VSSGQLP
QDGDHSQSSLGLDLFGCCFCCL*GRLHGYRTEAA
EARALSRSWLRSSPLPTPIPKPPHGSP*LLSAW
PSL*GTESSAAPSLSGGHPSPTGKAATCPTPC*
KGSGSPSPSGSCPPPKRAALSPAPQ*TEGRNASS
IWGPEPSRT*VLECSPALPTGPVPRGLGPPAGW
LPPWAAFSRQSEPNLPS*PSACGIWSLEPTTVQ
LLPPHSPG*DPQFPGDWIFPLLPLPQPHKPPRVC
QTCVGGSNEPIL*GRVLGQDPTPLVLHAAGGGS
VLLLPGGSSPATLPAPGLPQPSA*YFLGS*AAF
PSWGAHIGQYQEHLPPEALPLCATQPASY*PPTK
GAAGTDPAGEGVPALLPPGEQSHLYMEGLGGYP
*QIL*PGVCCEDPPPLVLPPPSQPYSG*VLCPS
GSLPQL*PGHLDH*HRSSHPQPHGPPLWKPKSSH
QA*TYSWADPQHDCPLL*PAISRTGLLCRGGEI
NLHQ*SVWSPT*HLARPCPLLLPESWG*TGQLL
QHQLSEERGSSVWRH*ERQGPGGAGLNWRNKYQL
HL*AQGRMSHPRALEGQMGGTPPCPTHLNTHYT
KHLLDL

Frame 1

QP*QPPDKLQWPALHIQTCLRGPTSECPVVSCP
RMGTTARAALVLTYLAVASAASEGGFTATGQRQ
LRPEHFQEVGYAAPPSPPLSRSLPMDHPDSSQHG
PPFEGQSQVQPPPSQEATPLQQEKLLPAQLPAE
KEVGPPLPQEAVPLQKELPSLQHPNEQKEGTPA
PFGDQSHPEPESWNAAQHCQQDRSQGGWGHRLDG
FPPGRPSPDNLNQICLPNRQHVVYGPWNLPQSS
YSHLTRQGETLNFLEIGYSRCCHCRSHTNRLEC
AKLVWEEAMSRFCEAEFSVKTRPHWCCTRQGEAR
FSCFQEEAPQPHYQLRACPSHQPDISSGLELPF
PPGVPTLDNIKNICHLRRFRSVPRNLPATDPLQ
RELLALIQLEREFQRCCRQGNNHTCTWKAWEDTL
DKYCDREYAVKTHHHLCCRHPPSPTRDECFARR
APYPNYDRDILTIDIGRVTPNLMGHLCGNQRVL
TKHKHIPGLIHNMTARCCDLPFPEQACCAEEEKL
TFINDLCGPRRNIWRDPALCCYLSPGDEQVNCF
NINYLRNVALVSGDTENAKGQGEQGSTGGTNIS
STSEPKEE*VTPEP*RVRWGEPHPAPPI*TLITL
NTSWIW

Frame 2

NRNSHQTSFSGRPFTSRLA*EDPPLSVQWSVAP
GWGPQPEQPWS*PIWLLLLLPLREASRLQDRGS
*GQSTFKKLATQLPPPHPYPEASPWITLTPLSM
ALPLRDRVKCSPLPLRRPPLSNRKSCYLPNSLLK
RKWVPLSLRKLSPSKKSCPLSSTPMNRRKERQL
HLGTRAIQNLSPGMQPSTANRTGPKGAGATGWM
ASPLGGLLQTI*TKSAFLTVSMWYMVPGTYHSPA
TPTSLARVRPSISWRLDIPAAATAAATQTA*SV
PNLCGRKQ*ADSVRPSSRSRPDPTGAARGRGRL
GSPASRRKLPSHTTSSGPAPAISLIFPRVLSCLS
LLGCPHWTISRTSAT*GASALCHATCQLLTPYK
GSCWH*SSWRGSSSAAAARGTITPVHGRPGRIP
LTNTVTGSML*RPTTTCVAATLPALLGMSALPVG
LLTPTMTGTS*PLTSVESPPTSWATSVETKEFS
PSINIFLG*STT*LPAAVTCHFQNRPAVQRRRN
*PSSMICVVPDVTSGETLPSAVT*VLGMNRSTAS
TSII*GTWL*CLETLRTPRARGSRAQLEEQISA
PPLSPRKNESPQSPRGSDGGNPTLPHPSEHSLH
*TPLGF

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