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Sequence NM_133272
TCCACCCAAGAGCAACCTGGAACTAAGTTATTCGGCAACGAACTGTTCCACTTTGTTGTGAGGCAATAGATGTGGAAATTCCCTGACGAGGGGCTCTGTCCTCATACTTCCTGCGGAGCTTATTGTCGTAAGAATATCTGTCATCCTGCTAATGTGCATTGAAAGGAGAGCAACGGGGCTGAGGCCGTGTCAGCACGATG
GACCCCAAACAGACCACCCTCCTGTGTCTTGGGGACTTTCCCATGCCTTTCATATCTGCCAAATCGAGTCCTGTGATTCCCTTGGATGGATCTGTGAAAA
TCCAGTGCCAGGCCATTCGTGAAGCTTACCTGACCCAGCTGATGATCATAAAAAACTCCACGTACCGAGAGATAGGCAGAAGACTGAAGTTTTGGAATGA
GACTGATCCTGAGTTCGTCATTGACCACATGGACGCAAACAAGGCAGGGCGCTATCAGTGCCAATATAGGATAGGGCACTACAGATTCCGGTACAGTGAC
ACCCTGGAGCTGGTAGTGACAGGCTTGTATGGCAAACCCTTCCTCTCTGCAGATCGGGGTCTGGTGTTGATGCCAGGAGAGAATATTTCCCTCACGTGCA
GCTCAGCACACATCCCATTTGATAGATTTTCACTGGCCAAGGAGGGAGAACTTTCTCTGCCACAGCACCAAAGTGGGGAACACCCGGCCAACTTCTCTTT
GGGTCCTGTGGACCTCAATGTCTCAGGGATCTACAGGTGCTACGGTTGGTACAACAGGAGCCCCTACCTGTGGTCCTTCCCCAGTAATGCCTTGGAGCTT
GTGGTCACAGACTCCATCCACCAAGATTACACGACGCAGAACTTGATCCGCATGGCCGTGGCAGGACTGGTCCTCGTGGCTCTCTTGGCCATACTGGTTG
AAAATTGGCACAGCCATACGGCACTGAACAAGGAAGCCTCGGCAGATGTGGCTGAACCGAGCTGGAGCCAACAGATGTGTCAGCCAGGATTGACCTTTGC
ACGAACACCAAGTGTCTGCAAGTAAACACCTGGAGGTGAAGGCAGAGAGGAGCCAGGACTGTGGAGTCCGACAAAGCTACTTGAAGGACACAAGAGAGAA
AAGCTCACTAAGAAGCTTGAATCTACTTTTTTTTTTTTTTGAGACAGAGTCTGGCTCTGTCACCCAGGCTGGAGTGCAGTGGAGCAATCTCGGCTCATTG
AACCTCTTGGGTTCAAGTGATTCTTGTGCCTCAGCCTCCCAAGTAGCTGGAATTACAGGCACATACCACTGCACCCAGCTAATTTTTGTATTTTTAGTAG
AGATGGGGTTTCACTGTGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCACCTTGGCCTCCCAAAGTGCTGAGATTATAGGCATGAG
CCACCACGCCTGGCCAGATGCATGTTCAAACCAATCAAATGGTGTTTTCTTATGCAGGACTGATCGATTTGCACCCACCTTTCTGCACATAAGTTATGGT
TTTCCATCTTATCTGTCTTCTGATTTTTTATATCCTGTTTAATTTCTTCCTTCATTGTTCTTCTCTTTTTTTATTTATTTTATTTATTTTTATTTTTATT
TTTATTTGAGACAGAGTCTCACTCTGTTGCCCAGG
- refseq_FCAR.F4 (forward primer)
- refseq_FCAR.R4 (reverse primer)
- Single exon skipping
Frame 0
STQEQPGTKLFGNELFHFVVRQ*MWKFPDEGLCPHTSCGAYCRKNICHPANVH*KESNGAEAVSARW
TPNRPPSCVLGTFPCLSYLPNRVL*FPWMDL*K
SSARPFVKLT*PS**S*KTPRTER*AED*SFGM
RLILSSSLTTWTQTRQGAISANIG*GTTDSGTVT
PWSW**QACMANPSSLQIGVWC*CQERIFPSRA
AQHTSHLIDFHWPRRENFLCHSTKVGNTRPTSL
WVLWTSMSQGSTGATVGTTGAPTCGPSPVMPWSL
WSQTPSTKITRRRT*SAWPWQDWSSWLSWPYWL
KIGTAIRH*TRKPRQMWLNRAGANRCVSQD*PL
HEHQVSASKHLEVKAERSQDCGVRQSYLKDTREK
SSLRSLNLLFFFFETESGSVTQAGVQWSNLGSL
NLLGSSDSCASASQVAGITGTYHCTQLIFVFLV
EMGFHCVGQAGLELLTSGDPPTLASQSAEIIGMS
HHAWPDACSNQSNGVFLCRTDRFAPTFLHISYG
FPSYLSSDFLYPV*FLPSLFFSFFIYFIYFYFY
FYLRQSLTLLPR
Frame 1
PPKSNLELSYSATNCSTLL*GNRCGNSLTRGSVLILPAELIVVRISVILLMCIERRATGLRPCQHD
GPQTDHPPVSWGLSHAFHICQIESCDSLGWICEN
PVPGHS*SLPDPADDHKKLHVPRDRQKTEVLE*
D*S*VRH*PHGRKQGRALSVPI*DRALQIPVQ*
HPGAGSDRLVWQTLPLCRSGSGVDARREYFPHVQ
LSTHPI**IFTGQGGRTFSATAPKWGTPGQLLF
GSCGPQCLRDLQVLRLVQQEPLPVVLPQ*CLGA
CGHRLHPPRLHDAELDPHGRGRTGPRGSLGHTG*
KLAQPYGTEQGSLGRCG*TELEPTDVSARIDLC
TNTKCLQVNTWR*RQRGARTVESDKAT*RTQER
KAH*EA*IYFFFFLRQSLALSPRLECSGAISAH*
TSWVQVILVPQPPK*LELQAHTTAPS*FLYF**
RWGFTVLARLVSNS*PQVIHPPWPPKVLRL*A*
ATTPGQMHVQTNQMVFSYAGLIDLHPPFCT*VMV
FHLICLLIFYILFNFFLHCSSLFLFILFIFIFI
FI*DRVSLCCP
Frame 2
HPRATWN*VIRQRTVPLCCEAIDVEIP*RGALSSYFLRSLLS*EYLSSC*CALKGEQRG*GRVSTM
DPKQTTLLCLGDFPMPFISAKSSPVIPLDGSVK
IQCQAIREAYLTQLMIIKNSTYREIGRRLKFWNE
TDPEFVIDHMDANKAGRYQCQYRIGHYRFRYSD
TLELVVTGLYGKPFLSADRGLVLMPGENISLTC
SSAHIPFDRFSLAKEGELSLPQHQSGEHPANFSL
GPVDLNVSGIYRCYGWYNRSPYLWSFPSNALEL
VVTDSIHQDYTTQNLIRMAVAGLVLVALLAILV
ENWHSHTALNKEASADVAEPSWSQQMCQPGLTFA
RTPSVCK*TPGGEGREEPGLWSPTKLLEGHKRE
KLTKKLESTFFFF*DRVWLCHPGWSAVEQSRLI
EPLGFK*FLCLSLPSSWNYRHIPLHPANFCIFSR
DGVSLCWPGWSRTPDLR*STHLGLPKC*DYRHE
PPRLARCMFKPIKWCFLMQD*SICTHLSAHKLW
FSILSVF*FFISCLISSFIVLLFFYLFYLFLFLF
LFETESHSVAQ
Sequence NM_133273
TCCACCCAAGAGCAACCTGGAACTAAGTTATTCGGCAACGAACTGTTCCACTTTGTTGTGAGGCAATAGATGTGGAAATTCCCTGACGAGGGGCTCTGTCCTCATACTTCCTGCGGAGCTTATTGTCGTAAGAATATCTGTCATCCTGCTAATGTGCATTGAAAGGAGAGCAACGGGGCTGAGGCCGTGTCAGCACGATG
GACCCCAAACAGACCACCCTCCTGTGTCTTGGGGACTTTCCCATGCCTTTCATATCTGCCAAATCGAGTCCTGTGATTCCCTTGGATGGATCTGTGAAAA
TCCAGTGCCAGGCCATTCGTGAAGCTTACCTGACCCAGCTGATGATCATAAAAAACTCCACGTACCGAGAGATAGGCAGAAGACTGAAGTTTTGGAATGA
GACTGATCCTGAGTTCGTCATTGACCACATGGACGCAAACAAGGCAGGGCGCTATCAGTGCCAATATAGGATAGGGCACTACAGATTCCGGTACAGTGAC
ACCCTGGAGCTGGTAGTGACAGACTCCATCCACCAAGATTACACGACGCAGAACTTGATCCGCATGGCCGTGGCAGGACTGGTCCTCGTGGCTCTCTTGG
CCATACTGGTTGAAAATTGGCACAGCCATACGGCACTGAACAAGGAAGCCTCGGCAGATGTGGCTGAACCGAGCTGGAGCCAACAGATGTGTCAGCCAGG
ATTGACCTTTGCACGAACACCAAGTGTCTGCAAGTAAACACCTGGAGGTGAAGGCAGAGAGGAGCCAGGACTGTGGAGTCCGACAAAGCTACTTGAAGGA
CACAAGAGAGAAAAGCTCACTAAGAAGCTTGAATCTACTTTTTTTTTTTTTTGAGACAGAGTCTGGCTCTGTCACCCAGGCTGGAGTGCAGTGGAGCAAT
CTCGGCTCATTGAACCTCTTGGGTTCAAGTGATTCTTGTGCCTCAGCCTCCCAAGTAGCTGGAATTACAGGCACATACCACTGCACCCAGCTAATTTTTG
TATTTTTAGTAGAGATGGGGTTTCACTGTGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCACCTTGGCCTCCCAAAGTGCTGAGAT
TATAGGCATGAGCCACCACGCCTGGCCAGATGCATGTTCAAACCAATCAAATGGTGTTTTCTTATGCAGGACTGATCGATTTGCACCCACCTTTCTGCAC
ATAAGTTATGGTTTTCCATCTTATCTGTCTTCTGATTTTTTATATCCTGTTTAATTTCTTCCTTCATTGTTCTTCTCTTTTTTTATTTATTTTATTTATT
TTTATTTTTATTTTTATTTGAGACAGAGTCTCACTCTGTTGCCCAGG
- refseq_FCAR.F4 (forward primer)
- refseq_FCAR.R4 (reverse primer)
- Single exon skipping
Frame 0
STQEQPGTKLFGNELFHFVVRQ*MWKFPDEGLCPHTSCGAYCRKNICHPANVH*KESNGAEAVSARW
TPNRPPSCVLGTFPCLSYLPNRVL*FPWMDL*K
SSARPFVKLT*PS**S*KTPRTER*AED*SFGM
RLILSSSLTTWTQTRQGAISANIG*GTTDSGTVT
PWSW**QTPSTKITRRRT*SAWPWQDWSSWLSW
PYWLKIGTAIRH*TRKPRQMWLNRAGANRCVSQ
D*PLHEHQVSASKHLEVKAERSQDCGVRQSYLKD
TREKSSLRSLNLLFFFFETESGSVTQAGVQWSN
LGSLNLLGSSDSCASASQVAGITGTYHCTQLIF
VFLVEMGFHCVGQAGLELLTSGDPPTLASQSAEI
IGMSHHAWPDACSNQSNGVFLCRTDRFAPTFLH
ISYGFPSYLSSDFLYPV*FLPSLFFSFFIYFIY
FYFYFYLRQSLTLLPR
Frame 1
PPKSNLELSYSATNCSTLL*GNRCGNSLTRGSVLILPAELIVVRISVILLMCIERRATGLRPCQHD
GPQTDHPPVSWGLSHAFHICQIESCDSLGWICEN
PVPGHS*SLPDPADDHKKLHVPRDRQKTEVLE*
D*S*VRH*PHGRKQGRALSVPI*DRALQIPVQ*
HPGAGSDRLHPPRLHDAELDPHGRGRTGPRGSLG
HTG*KLAQPYGTEQGSLGRCG*TELEPTDVSAR
IDLCTNTKCLQVNTWR*RQRGARTVESDKAT*R
TQERKAH*EA*IYFFFFLRQSLALSPRLECSGAI
SAH*TSWVQVILVPQPPK*LELQAHTTAPS*FL
YF**RWGFTVLARLVSNS*PQVIHPPWPPKVLR
L*A*ATTPGQMHVQTNQMVFSYAGLIDLHPPFCT
*VMVFHLICLLIFYILFNFFLHCSSLFLFILFI
FIFIFI*DRVSLCCP
Frame 2
HPRATWN*VIRQRTVPLCCEAIDVEIP*RGALSSYFLRSLLS*EYLSSC*CALKGEQRG*GRVSTM
DPKQTTLLCLGDFPMPFISAKSSPVIPLDGSVK
IQCQAIREAYLTQLMIIKNSTYREIGRRLKFWNE
TDPEFVIDHMDANKAGRYQCQYRIGHYRFRYSD
TLELVVTDSIHQDYTTQNLIRMAVAGLVLVALL
AILVENWHSHTALNKEASADVAEPSWSQQMCQPG
LTFARTPSVCK*TPGGEGREEPGLWSPTKLLEG
HKREKLTKKLESTFFFF*DRVWLCHPGWSAVEQ
SRLIEPLGFK*FLCLSLPSSWNYRHIPLHPANFC
IFSRDGVSLCWPGWSRTPDLR*STHLGLPKC*D
YRHEPPRLARCMFKPIKWCFLMQD*SICTHLSA
HKLWFSILSVF*FFISCLISSFIVLLFFYLFYLF
LFLFLFETESHSVAQ
Sequence NM_133274
TCCACCCAAGAGCAACCTGGAACTAAGTTATTCGGCAACGAACTGTTCCACTTTGTTGTGAGGCAATAGATGTGGAAATTCCCTGACGAGGGGCTCTGTCCTCATACTTCCTGCGGAGCTTATTGTCGTAAGAATATCTGTCATCCTGCTAATGTGCATTGAAAGGAGAGCAACGGGGCTGAGGCCGTGTCAGCACGATG
GACCCCAAACAGACCACCCTCCTGTGTCTTGGGGACTTTCCCATGCCTTTCATATCTGCCAAATCGAGTCCTGTGATTCCCTTGGATGGATCTGTGAAAA
TCCAGTGCCAGGCCATTCGTGAAGCTTACCTGACCCAGCTGATGATCATAAAAAACTCCACGTACCGAGAGATAGGCAGAAGACTGAAGTTTTGGAATGA
GACTGATCCTGAGTTCGTCATTGACCACATGGACGCAAACAAGGCAGGGCGCTATCAGTGCCAATATAGGATAGGGCACTACAGATTCCGGTACAGTGAC
ACCCTGGAGCTGGTAGTGACAGGCTTGTATGGCAAACCCTTCCTCTCTGCAGATCGGGGTCTGGTGTTGATGCCAGGAGAGAATATTTCCCTCACGTGCA
GCTCAGCACACATCCCATTTGATAGATTTTCACTGGCCAAGGAGGGAGAACTTTCTCTGCCACAGCACCAAAGTGGGGAACACCCGGCCAACTTCTCTTT
GGGTCCTGTGGACCTCAATGTCTCAGGGATCTACAGACTCCATCCACCAAGATTACACGACGCAGAACTTGATCCGCATGGCCGTGGCAGGACTGGTCCT
CGTGGCTCTCTTGGCCATACTGGTTGAAAATTGGCACAGCCATACGGCACTGAACAAGGAAGCCTCGGCAGATGTGGCTGAACCGAGCTGGAGCCAACAG
ATGTGTCAGCCAGGATTGACCTTTGCACGAACACCAAGTGTCTGCAAGTAAACACCTGGAGGTGAAGGCAGAGAGGAGCCAGGACTGTGGAGTCCGACAA
AGCTACTTGAAGGACACAAGAGAGAAAAGCTCACTAAGAAGCTTGAATCTACTTTTTTTTTTTTTTGAGACAGAGTCTGGCTCTGTCACCCAGGCTGGAG
TGCAGTGGAGCAATCTCGGCTCATTGAACCTCTTGGGTTCAAGTGATTCTTGTGCCTCAGCCTCCCAAGTAGCTGGAATTACAGGCACATACCACTGCAC
CCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACTGTGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCACCTTGGCCTCC
CAAAGTGCTGAGATTATAGGCATGAGCCACCACGCCTGGCCAGATGCATGTTCAAACCAATCAAATGGTGTTTTCTTATGCAGGACTGATCGATTTGCAC
CCACCTTTCTGCACATAAGTTATGGTTTTCCATCTTATCTGTCTTCTGATTTTTTATATCCTGTTTAATTTCTTCCTTCATTGTTCTTCTCTTTTTTTAT
TTATTTTATTTATTTTTATTTTTATTTTTATTTGAGACAGAGTCTCACTCTGTTGCCCAGG
- refseq_FCAR.F4 (forward primer)
- refseq_FCAR.R4 (reverse primer)
- Single exon skipping
Frame 0
STQEQPGTKLFGNELFHFVVRQ*MWKFPDEGLCPHTSCGAYCRKNICHPANVH*KESNGAEAVSARW
TPNRPPSCVLGTFPCLSYLPNRVL*FPWMDL*K
SSARPFVKLT*PS**S*KTPRTER*AED*SFGM
RLILSSSLTTWTQTRQGAISANIG*GTTDSGTVT
PWSW**QACMANPSSLQIGVWC*CQERIFPSRA
AQHTSHLIDFHWPRRENFLCHSTKVGNTRPTSL
WVLWTSMSQGSTDSIHQDYTTQNLIRMAVAGLVL
VALLAILVENWHSHTALNKEASADVAEPSWSQQ
MCQPGLTFARTPSVCK*TPGGEGREEPGLWSPT
KLLEGHKREKLTKKLESTFFFF*DRVWLCHPGWS
AVEQSRLIEPLGFK*FLCLSLPSSWNYRHIPLH
PANFCIFSRDGVSLCWPGWSRTPDLR*STHLGL
PKC*DYRHEPPRLARCMFKPIKWCFLMQD*SICT
HLSAHKLWFSILSVF*FFISCLISSFIVLLFFY
LFYLFLFLFLFETESHSVAQ
Frame 1
PPKSNLELSYSATNCSTLL*GNRCGNSLTRGSVLILPAELIVVRISVILLMCIERRATGLRPCQHD
GPQTDHPPVSWGLSHAFHICQIESCDSLGWICEN
PVPGHS*SLPDPADDHKKLHVPRDRQKTEVLE*
D*S*VRH*PHGRKQGRALSVPI*DRALQIPVQ*
HPGAGSDRLVWQTLPLCRSGSGVDARREYFPHVQ
LSTHPI**IFTGQGGRTFSATAPKWGTPGQLLF
GSCGPQCLRDLQTPSTKITRRRT*SAWPWQDWS
SWLSWPYWLKIGTAIRH*TRKPRQMWLNRAGANR
CVSQD*PLHEHQVSASKHLEVKAERSQDCGVRQ
SYLKDTREKSSLRSLNLLFFFFETESGSVTQAG
VQWSNLGSLNLLGSSDSCASASQVAGITGTYHCT
QLIFVFLVEMGFHCVGQAGLELLTSGDPPTLAS
QSAEIIGMSHHAWPDACSNQSNGVFLCRTDRFA
PTFLHISYGFPSYLSSDFLYPV*FLPSLFFSFFI
YFIYFYFYFYLRQSLTLLPR
Frame 2
HPRATWN*VIRQRTVPLCCEAIDVEIP*RGALSSYFLRSLLS*EYLSSC*CALKGEQRG*GRVSTM
DPKQTTLLCLGDFPMPFISAKSSPVIPLDGSVK
IQCQAIREAYLTQLMIIKNSTYREIGRRLKFWNE
TDPEFVIDHMDANKAGRYQCQYRIGHYRFRYSD
TLELVVTGLYGKPFLSADRGLVLMPGENISLTC
SSAHIPFDRFSLAKEGELSLPQHQSGEHPANFSL
GPVDLNVSGIYRLHPPRLHDAELDPHGRGRTGP
RGSLGHTG*KLAQPYGTEQGSLGRCG*TELEPT
DVSARIDLCTNTKCLQVNTWR*RQRGARTVESDK
AT*RTQERKAH*EA*IYFFFFLRQSLALSPRLE
CSGAISAH*TSWVQVILVPQPPK*LELQAHTTA
PS*FLYF**RWGFTVLARLVSNS*PQVIHPPWPP
KVLRL*A*ATTPGQMHVQTNQMVFSYAGLIDLH
PPFCT*VMVFHLICLLIFYILFNFFLHCSSLFL
FILFIFIFIFI*DRVSLCCP
Sequence NM_133278
TCCACCCAAGAGCAACCTGGAACTAAGTTATTCGGCAACGAACTGTTCCACTTTGTTGTGAGGCAATAGATGTGGAAATTCCCTGACGAGGGGCTCTGTCCTCATACTTCCTGCGGAGCTTATTGTCGTAAGAATATCTGTCATCCTGCTAATGTGCATTGAAAGGAGAGCAACGGGGCTGAGGCCGTGTCAGCACGATG
GACCCCAAACAGACCACCCTCCTGTGTCTTGGGGACTTTCCCATGCCTTTCATATCTGCCAAATCGAGTCCTGTGATTCCCTTGGATGGATCTGTGAAAA
TCCAGTGCCAGGCCATTCGTGAAGCTTACCTGACCCAGCTGATGATCATAAAAAACTCCACGTACCGAGAGATAGGCAGAAGACTGAAGTTTTGGAATGA
GACTGATCCTGAGTTCGTCATTGACCACATGGACGCAAACAAGGCAGGGCGCTATCAGTGCCAATATAGGATAGGGCACTACAGATTCCGGTACAGTGAC
ACCCTGGAGCTGGTAGTGACAGGCTTGTATGGCAAACCCTTCCTCTCTGCAGATCGGGGTCTGGTGTTGATGCCAGGAGAGAATATTTCCCTCACGTGCA
GCTCAGCACACATCCCATTTGATAGATTTTCACTGGCCAAGGAGGGAGAACTTTCTCTGCCACAGCACCAAAGTGGGGAACACCCGGCCAACTTCTCTTT
GGGTCCTGTGGACCTCAATGTCTCAGGGATCTACAGGTGCTACGACTCCATCCACCAAGATTACACGACGCAGAACTTGATCCGCATGGCCGTGGCAGGA
CTGGTCCTCGTGGCTCTCTTGGCCATACTGGTTGAAAATTGGCACAGCCATACGGCACTGAACAAGGAAGCCTCGGCAGATGTGGCTGAACCGAGCTGGA
GCCAACAGATGTGTCAGCCAGGATTGACCTTTGCACGAACACCAAGTGTCTGCAAGTAAACACCTGGAGGTGAAGGCAGAGAGGAGCCAGGACTGTGGAG
TCCGACAAAGCTACTTGAAGGACACAAGAGAGAAAAGCTCACTAAGAAGCTTGAATCTACTTTTTTTTTTTTTTGAGACAGAGTCTGGCTCTGTCACCCA
GGCTGGAGTGCAGTGGAGCAATCTCGGCTCATTGAACCTCTTGGGTTCAAGTGATTCTTGTGCCTCAGCCTCCCAAGTAGCTGGAATTACAGGCACATAC
CACTGCACCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACTGTGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCACCT
TGGCCTCCCAAAGTGCTGAGATTATAGGCATGAGCCACCACGCCTGGCCAGATGCATGTTCAAACCAATCAAATGGTGTTTTCTTATGCAGGACTGATCG
ATTTGCACCCACCTTTCTGCACATAAGTTATGGTTTTCCATCTTATCTGTCTTCTGATTTTTTATATCCTGTTTAATTTCTTCCTTCATTGTTCTTCTCT
TTTTTTATTTATTTTATTTATTTTTATTTTTATTTTTATTTGAGACAGAGTCTCACTCTGTTGCCCAGG
- refseq_FCAR.F4 (forward primer)
- refseq_FCAR.R4 (reverse primer)
- Single exon skipping
Frame 0
STQEQPGTKLFGNELFHFVVRQ*MWKFPDEGLCPHTSCGAYCRKNICHPANVH*KESNGAEAVSARW
TPNRPPSCVLGTFPCLSYLPNRVL*FPWMDL*K
SSARPFVKLT*PS**S*KTPRTER*AED*SFGM
RLILSSSLTTWTQTRQGAISANIG*GTTDSGTVT
PWSW**QACMANPSSLQIGVWC*CQERIFPSRA
AQHTSHLIDFHWPRRENFLCHSTKVGNTRPTSL
WVLWTSMSQGSTGATTPSTKITRRRT*SAWPWQD
WSSWLSWPYWLKIGTAIRH*TRKPRQMWLNRAG
ANRCVSQD*PLHEHQVSASKHLEVKAERSQDCG
VRQSYLKDTREKSSLRSLNLLFFFFETESGSVTQ
AGVQWSNLGSLNLLGSSDSCASASQVAGITGTY
HCTQLIFVFLVEMGFHCVGQAGLELLTSGDPPT
LASQSAEIIGMSHHAWPDACSNQSNGVFLCRTDR
FAPTFLHISYGFPSYLSSDFLYPV*FLPSLFFS
FFIYFIYFYFYFYLRQSLTLLPR
Frame 1
PPKSNLELSYSATNCSTLL*GNRCGNSLTRGSVLILPAELIVVRISVILLMCIERRATGLRPCQHD
GPQTDHPPVSWGLSHAFHICQIESCDSLGWICEN
PVPGHS*SLPDPADDHKKLHVPRDRQKTEVLE*
D*S*VRH*PHGRKQGRALSVPI*DRALQIPVQ*
HPGAGSDRLVWQTLPLCRSGSGVDARREYFPHVQ
LSTHPI**IFTGQGGRTFSATAPKWGTPGQLLF
GSCGPQCLRDLQVLRLHPPRLHDAELDPHGRGR
TGPRGSLGHTG*KLAQPYGTEQGSLGRCG*TELE
PTDVSARIDLCTNTKCLQVNTWR*RQRGARTVE
SDKAT*RTQERKAH*EA*IYFFFFLRQSLALSP
RLECSGAISAH*TSWVQVILVPQPPK*LELQAHT
TAPS*FLYF**RWGFTVLARLVSNS*PQVIHPP
WPPKVLRL*A*ATTPGQMHVQTNQMVFSYAGLI
DLHPPFCT*VMVFHLICLLIFYILFNFFLHCSSL
FLFILFIFIFIFI*DRVSLCCP
Frame 2
HPRATWN*VIRQRTVPLCCEAIDVEIP*RGALSSYFLRSLLS*EYLSSC*CALKGEQRG*GRVSTM
DPKQTTLLCLGDFPMPFISAKSSPVIPLDGSVK
IQCQAIREAYLTQLMIIKNSTYREIGRRLKFWNE
TDPEFVIDHMDANKAGRYQCQYRIGHYRFRYSD
TLELVVTGLYGKPFLSADRGLVLMPGENISLTC
SSAHIPFDRFSLAKEGELSLPQHQSGEHPANFSL
GPVDLNVSGIYRCYDSIHQDYTTQNLIRMAVAG
LVLVALLAILVENWHSHTALNKEASADVAEPSW
SQQMCQPGLTFARTPSVCK*TPGGEGREEPGLWS
PTKLLEGHKREKLTKKLESTFFFF*DRVWLCHP
GWSAVEQSRLIEPLGFK*FLCLSLPSSWNYRHI
PLHPANFCIFSRDGVSLCWPGWSRTPDLR*STHL
GLPKC*DYRHEPPRLARCMFKPIKWCFLMQD*S
ICTHLSAHKLWFSILSVF*FFISCLISSFIVLL
FFYLFYLFLFLFLFETESHSVAQ
Sequence NM_133280
TCCACCCAAGAGCAACCTGGAACTAAGTTATTCGGCAACGAACTGTTCCACTTTGTTGTGAGGCAATAGATGTGGAAATTCCCTGACGAGGGGCTCTGTCCTCATACTTCCTGCGGAGCTTATTGTCGTAAGAATATCTGTCATCCTGCTAATGTGCATTGAAAGGAGAGCAACGGGGCTGAGGCCGTGTCAGCACGATG
GACCCCAAACAGACCACCCTCCTGTGTCTTGGGGACTTTCCCATGCCTTTCATATCTGCCAAATCGAGTCCTGTGATTCCCTTGGATGGATCTGTGAAAA
TCCAGTGCCAGGCCATTCGTGAAGCTTACCTGACCCAGCTGATGATCATAAAAAACTCCACGTACCGAGAGATAGGCAGAAGACTGAAGTTTTGGAATGA
GACTGATCCTGAGTTCGTCATTGACCACATGGACGCAAACAAGGCAGGGCGCTATCAGTGCCAATATAGGATAGGGCACTACAGATTCCGGTACAGTGAC
ACCCTGGAGCTGGTAGTGACAGGCTTGTATGGCAAACCCTTCCTCTCTGCAGATCGGGGTCTGGTGTTGATGCCAGGAGAGAATATTTCCCTCACGTGCA
GCTCAGCACACATCCCATTTGATAGATTTTCACTGGCCAAGGAGGGAGAACTTTCTCTGCCACAGCACCAAAGTGGGGAACACCCGGCCAACTTCTCTTT
GGGTCCTGTGGACCTCAATGTCTCAGGGATCTACAGGTGCTACGGTTGGTACAACAGGAGCCCCTACCTGTGGTCCTTCCCCAGTAATGCCTTGGAGCTT
GTGGTCACAGGTAGGTACCGCCCAGTCCAGCCCTGTGTCTGGGTTGGCTGTCCAGGGCCTTGCCACCGGGCAGGAATATGAAGACGTGCACTGAGAGTGA
AGTGAAGAGAGGCAAAGGCTCTCACTCCAGGACAGTGGAGAGAGAAAGGCTTCCCCACCACACTTTCCGCTTTCACTTCCTCGCTAGAGTTCTCCAGACA
GGGTTCATTGAAAACTTAGTCTGTGGAGAACAGAAGGGCTAACTCAGTTTGTTTCATTTTATTTATTTCATTTTATTTTCCGGGATAGAGTCTTGCTCTT
TCGCCAAGGCTGGAGTGCAGTGGCACGATCTCGACTCACTGCAACCTTCGCCTCCCAGGAGAATCACTTGAACCCAGGAGGCAGCTGTTGCAGTGAGCCA
AGATCACGCCATTGCACTCCAGCCTGGGTGACAGAACGAGACTCCACCTCAAGAAAAAAAAAAAAAA
- refseq_FCAR.F4 (forward primer)
- refseq_FCAR.R4 (reverse primer)
- Single exon skipping
Frame 0
STQEQPGTKLFGNELFHFVVRQ*MWKFPDEGLCPHTSCGAYCRKNICHPANVH*KESNGAEAVSARW
TPNRPPSCVLGTFPCLSYLPNRVL*FPWMDL*K
SSARPFVKLT*PS**S*KTPRTER*AED*SFGM
RLILSSSLTTWTQTRQGAISANIG*GTTDSGTVT
PWSW**QACMANPSSLQIGVWC*CQERIFPSRA
AQHTSHLIDFHWPRRENFLCHSTKVGNTRPTSL
WVLWTSMSQGSTGATVGTTGAPTCGPSPVMPWSL
WSQVGTAQSSPVSGLAVQGLATGQEYEDVH*E*
SEERQRLSLQDSGERKASPPHFPLSLPR*SSPD
RVH*KLSLWRTEGLTQFVSFYLFHFIFRDRVLLF
RQGWSAVARSRLTATFASQENHLNPGGSCCSEP
RSRHCTPAWVTERDSTSRKKKK
Frame 1
PPKSNLELSYSATNCSTLL*GNRCGNSLTRGSVLILPAELIVVRISVILLMCIERRATGLRPCQHD
GPQTDHPPVSWGLSHAFHICQIESCDSLGWICEN
PVPGHS*SLPDPADDHKKLHVPRDRQKTEVLE*
D*S*VRH*PHGRKQGRALSVPI*DRALQIPVQ*
HPGAGSDRLVWQTLPLCRSGSGVDARREYFPHVQ
LSTHPI**IFTGQGGRTFSATAPKWGTPGQLLF
GSCGPQCLRDLQVLRLVQQEPLPVVLPQ*CLGA
CGHR*VPPSPALCLGWLSRALPPGRNMKTCTESE
VKRGKGSHSRTVERERLPHHTFRFHFLARVLQT
GFIENLVCGEQKG*LSLFHFIYFILFSGIESCS
FAKAGVQWHDLDSLQPSPPRRIT*TQEAAVAVSQ
DHAIALQPG*QNETPPQEKKKK
Frame 2
HPRATWN*VIRQRTVPLCCEAIDVEIP*RGALSSYFLRSLLS*EYLSSC*CALKGEQRG*GRVSTM
DPKQTTLLCLGDFPMPFISAKSSPVIPLDGSVK
IQCQAIREAYLTQLMIIKNSTYREIGRRLKFWNE
TDPEFVIDHMDANKAGRYQCQYRIGHYRFRYSD
TLELVVTGLYGKPFLSADRGLVLMPGENISLTC
SSAHIPFDRFSLAKEGELSLPQHQSGEHPANFSL
GPVDLNVSGIYRCYGWYNRSPYLWSFPSNALEL
VVTGRYRPVQPCVWVGCPGPCHRAGI*RRALRV
K*REAKALTPGQWREKGFPTTLSAFTSSLEFSRQ
GSLKT*SVENRRANSVCFILFISFYFPG*SLAL
SPRLECSGTISTHCNLRLPGESLEPRRQLLQ*A
KITPLHSSLGDRTRLHLKKKKK
Sequence NM_002000
TCCACCCAAGAGCAACCTGGAACTAAGTTATTCGGCAACGAACTGTTCCACTTTGTTGTGAGGCAATAGATGTGGAAATTCCCTGACGAGGGGCTCTGTCCTCATACTTCCTGCGGAGCTTATTGTCGTAAGAATATCTGTCATCCTGCTAATGTGCATTGAAAGGAGAGCAACGGGGCTGAGGCCGTGTCAGCACGATG
GACCCCAAACAGACCACCCTCCTGTGTCTTGTGCTCTGTCTGGGCCAGAGGATTCAGGCACAGGAAGGGGACTTTCCCATGCCTTTCATATCTGCCAAAT
CGAGTCCTGTGATTCCCTTGGATGGATCTGTGAAAATCCAGTGCCAGGCCATTCGTGAAGCTTACCTGACCCAGCTGATGATCATAAAAAACTCCACGTA
CCGAGAGATAGGCAGAAGACTGAAGTTTTGGAATGAGACTGATCCTGAGTTCGTCATTGACCACATGGACGCAAACAAGGCAGGGCGCTATCAGTGCCAA
TATAGGATAGGGCACTACAGATTCCGGTACAGTGACACCCTGGAGCTGGTAGTGACAGGCTTGTATGGCAAACCCTTCCTCTCTGCAGATCGGGGTCTGG
TGTTGATGCCAGGAGAGAATATTTCCCTCACGTGCAGCTCAGCACACATCCCATTTGATAGATTTTCACTGGCCAAGGAGGGAGAACTTTCTCTGCCACA
GCACCAAAGTGGGGAACACCCGGCCAACTTCTCTTTGGGTCCTGTGGACCTCAATGTCTCAGGGATCTACAGGTGCTACGGTTGGTACAACAGGAGCCCC
TACCTGTGGTCCTTCCCCAGTAATGCCTTGGAGCTTGTGGTCACAGACTCCATCCACCAAGATTACACGACGCAGAACTTGATCCGCATGGCCGTGGCAG
GACTGGTCCTCGTGGCTCTCTTGGCCATACTGGTTGAAAATTGGCACAGCCATACGGCACTGAACAAGGAAGCCTCGGCAGATGTGGCTGAACCGAGCTG
GAGCCAACAGATGTGTCAGCCAGGATTGACCTTTGCACGAACACCAAGTGTCTGCAAGTAAACACCTGGAGGTGAAGGCAGAGAGGAGCCAGGACTGTGG
AGTCCGACAAAGCTACTTGAAGGACACAAGAGAGAAAAGCTCACTAAGAAGCTTGAATCTACTTTTTTTTTTTTTTGAGACAGAGTCTGGCTCTGTCACC
CAGGCTGGAGTGCAGTGGAGCAATCTCGGCTCATTGAACCTCTTGGGTTCAAGTGATTCTTGTGCCTCAGCCTCCCAAGTAGCTGGAATTACAGGCACAT
ACCACTGCACCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACTGTGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGGTGATCCACCCAC
CTTGGCCTCCCAAAGTGCTGAGATTATAGGCATGAGCCACCACGCCTGGCCAGATGCATGTTCAAACCAATCAAATGGTGTTTTCTTATGCAGGACTGAT
CGATTTGCACCCACCTTTCTGCACATAAGTTATGGTTTTCCATCTTATCTGTCTTCTGATTTTTTATATCCTGTTTAATTTCTTCCTTCATTGTTCTTCT
CTTTTTTTATTTATTTTATTTATTTTTATTTTTATTTTTATTTGAGACAGAGTCTCACTCTGTTGCCCAGG
- refseq_FCAR.F4 (forward primer)
- refseq_FCAR.R4 (reverse primer)
- Single exon skipping
Frame 0
STQEQPGTKLFGNELFHFVVRQ*MWKFPDEGLCPHTSCGAYCRKNICHPANVH*KESNGAEAVSARW
TPNRPPSCVLCSVWARGFRHRKGTFPCLSYLPN
RVL*FPWMDL*KSSARPFVKLT*PS**S*KTPR
TER*AED*SFGMRLILSSSLTTWTQTRQGAISAN
IG*GTTDSGTVTPWSW**QACMANPSSLQIGVW
C*CQERIFPSRAAQHTSHLIDFHWPRRENFLCH
STKVGNTRPTSLWVLWTSMSQGSTGATVGTTGAP
TCGPSPVMPWSLWSQTPSTKITRRRT*SAWPWQ
DWSSWLSWPYWLKIGTAIRH*TRKPRQMWLNRA
GANRCVSQD*PLHEHQVSASKHLEVKAERSQDCG
VRQSYLKDTREKSSLRSLNLLFFFFETESGSVT
QAGVQWSNLGSLNLLGSSDSCASASQVAGITGT
YHCTQLIFVFLVEMGFHCVGQAGLELLTSGDPPT
LASQSAEIIGMSHHAWPDACSNQSNGVFLCRTD
RFAPTFLHISYGFPSYLSSDFLYPV*FLPSLFF
SFFIYFIYFYFYFYLRQSLTLLPR
Frame 1
PPKSNLELSYSATNCSTLL*GNRCGNSLTRGSVLILPAELIVVRISVILLMCIERRATGLRPCQHD
GPQTDHPPVSCALSGPEDSGTGRGLSHAFHICQI
ESCDSLGWICENPVPGHS*SLPDPADDHKKLHV
PRDRQKTEVLE*D*S*VRH*PHGRKQGRALSVP
I*DRALQIPVQ*HPGAGSDRLVWQTLPLCRSGSG
VDARREYFPHVQLSTHPI**IFTGQGGRTFSAT
APKWGTPGQLLFGSCGPQCLRDLQVLRLVQQEP
LPVVLPQ*CLGACGHRLHPPRLHDAELDPHGRGR
TGPRGSLGHTG*KLAQPYGTEQGSLGRCG*TEL
EPTDVSARIDLCTNTKCLQVNTWR*RQRGARTV
ESDKAT*RTQERKAH*EA*IYFFFFLRQSLALSP
RLECSGAISAH*TSWVQVILVPQPPK*LELQAH
TTAPS*FLYF**RWGFTVLARLVSNS*PQVIHP
PWPPKVLRL*A*ATTPGQMHVQTNQMVFSYAGLI
DLHPPFCT*VMVFHLICLLIFYILFNFFLHCSS
LFLFILFIFIFIFI*DRVSLCCP
Frame 2
HPRATWN*VIRQRTVPLCCEAIDVEIP*RGALSSYFLRSLLS*EYLSSC*CALKGEQRG*GRVSTM
DPKQTTLLCLVLCLGQRIQAQEGDFPMPFISAK
SSPVIPLDGSVKIQCQAIREAYLTQLMIIKNSTY
REIGRRLKFWNETDPEFVIDHMDANKAGRYQCQ
YRIGHYRFRYSDTLELVVTGLYGKPFLSADRGL
VLMPGENISLTCSSAHIPFDRFSLAKEGELSLPQ
HQSGEHPANFSLGPVDLNVSGIYRCYGWYNRSP
YLWSFPSNALELVVTDSIHQDYTTQNLIRMAVA
GLVLVALLAILVENWHSHTALNKEASADVAEPSW
SQQMCQPGLTFARTPSVCK*TPGGEGREEPGLW
SPTKLLEGHKREKLTKKLESTFFFF*DRVWLCH
PGWSAVEQSRLIEPLGFK*FLCLSLPSSWNYRHI
PLHPANFCIFSRDGVSLCWPGWSRTPDLR*STH
LGLPKC*DYRHEPPRLARCMFKPIKWCFLMQD*
SICTHLSAHKLWFSILSVF*FFISCLISSFIVLL
FFYLFYLFLFLFLFETESHSVAQ
Sequence NM_133271
TCCACCCAAGAGCAACCTGGAACTAAGTTATTCGGCAACGAACTGTTCCACTTTGTTGTGAGGCAATAGATGTGGAAATTCCCTGACGAGGGGCTCTGTCCTCATACTTCCTGCGGAGCTTATTGTCGTAAGAATATCTGTCATCCTGCTAATGTGCATTGAAAGGAGAGCAACGGGGCTGAGGCCGTGTCAGCACGATG
GACCCCAAACAGACCACCCTCCTGTGTCTTGTGCTCTGTCTGGGCCAGAGGATTCAGGCACAGGAAGGGGACTTTCCCATGCCTTTCATATCTGCCAAAT
CGAGTCCTGTGATTCCCTTGGATGGATCTGTGAAAATCCAGTGCCAGGCCATTCGTGAAGCTTACCTGACCCAGCTGATGATCATAAAAAACTCCACGTA
CCGAGAGATAGGCAGAAGACTGAAGTTTTGGAATGAGACTGATCCTGAGTTCGTCATTGACCACATGGACGCAAACAAGGCAGGGCGCTATCAGTGCCAA
TATAGGATAGGGCACTACAGATTCCGGTACAGTGACACCCTGGAGCTGGTAGTGACAGACTCCATCCACCAAGATTACACGACGCAGAACTTGATCCGCA
TGGCCGTGGCAGGACTGGTCCTCGTGGCTCTCTTGGCCATACTGGTTGAAAATTGGCACAGCCATACGGCACTGAACAAGGAAGCCTCGGCAGATGTGGC
TGAACCGAGCTGGAGCCAACAGATGTGTCAGCCAGGATTGACCTTTGCACGAACACCAAGTGTCTGCAAGTAAACACCTGGAGGTGAAGGCAGAGAGGAG
CCAGGACTGTGGAGTCCGACAAAGCTACTTGAAGGACACAAGAGAGAAAAGCTCACTAAGAAGCTTGAATCTACTTTTTTTTTTTTTTGAGACAGAGTCT
GGCTCTGTCACCCAGGCTGGAGTGCAGTGGAGCAATCTCGGCTCATTGAACCTCTTGGGTTCAAGTGATTCTTGTGCCTCAGCCTCCCAAGTAGCTGGAA
TTACAGGCACATACCACTGCACCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACTGTGTTGGCCAGGCTGGTCTCGAACTCCTGACCTCAGG
TGATCCACCCACCTTGGCCTCCCAAAGTGCTGAGATTATAGGCATGAGCCACCACGCCTGGCCAGATGCATGTTCAAACCAATCAAATGGTGTTTTCTTA
TGCAGGACTGATCGATTTGCACCCACCTTTCTGCACATAAGTTATGGTTTTCCATCTTATCTGTCTTCTGATTTTTTATATCCTGTTTAATTTCTTCCTT
CATTGTTCTTCTCTTTTTTTATTTATTTTATTTATTTTTATTTTTATTTTTATTTGAGACAGAGTCTCACTCTGTTGCCCAGG
- refseq_FCAR.F4 (forward primer)
- refseq_FCAR.R4 (reverse primer)
- Single exon skipping
Frame 0
STQEQPGTKLFGNELFHFVVRQ*MWKFPDEGLCPHTSCGAYCRKNICHPANVH*KESNGAEAVSARW
TPNRPPSCVLCSVWARGFRHRKGTFPCLSYLPN
RVL*FPWMDL*KSSARPFVKLT*PS**S*KTPR
TER*AED*SFGMRLILSSSLTTWTQTRQGAISAN
IG*GTTDSGTVTPWSW**QTPSTKITRRRT*SA
WPWQDWSSWLSWPYWLKIGTAIRH*TRKPRQMW
LNRAGANRCVSQD*PLHEHQVSASKHLEVKAERS
QDCGVRQSYLKDTREKSSLRSLNLLFFFFETES
GSVTQAGVQWSNLGSLNLLGSSDSCASASQVAG
ITGTYHCTQLIFVFLVEMGFHCVGQAGLELLTSG
DPPTLASQSAEIIGMSHHAWPDACSNQSNGVFL
CRTDRFAPTFLHISYGFPSYLSSDFLYPV*FLP
SLFFSFFIYFIYFYFYFYLRQSLTLLPR
Frame 1
PPKSNLELSYSATNCSTLL*GNRCGNSLTRGSVLILPAELIVVRISVILLMCIERRATGLRPCQHD
GPQTDHPPVSCALSGPEDSGTGRGLSHAFHICQI
ESCDSLGWICENPVPGHS*SLPDPADDHKKLHV
PRDRQKTEVLE*D*S*VRH*PHGRKQGRALSVP
I*DRALQIPVQ*HPGAGSDRLHPPRLHDAELDPH
GRGRTGPRGSLGHTG*KLAQPYGTEQGSLGRCG
*TELEPTDVSARIDLCTNTKCLQVNTWR*RQRG
ARTVESDKAT*RTQERKAH*EA*IYFFFFLRQSL
ALSPRLECSGAISAH*TSWVQVILVPQPPK*LE
LQAHTTAPS*FLYF**RWGFTVLARLVSNS*PQ
VIHPPWPPKVLRL*A*ATTPGQMHVQTNQMVFSY
AGLIDLHPPFCT*VMVFHLICLLIFYILFNFFL
HCSSLFLFILFIFIFIFI*DRVSLCCP
Frame 2
HPRATWN*VIRQRTVPLCCEAIDVEIP*RGALSSYFLRSLLS*EYLSSC*CALKGEQRG*GRVSTM
DPKQTTLLCLVLCLGQRIQAQEGDFPMPFISAK
SSPVIPLDGSVKIQCQAIREAYLTQLMIIKNSTY
REIGRRLKFWNETDPEFVIDHMDANKAGRYQCQ
YRIGHYRFRYSDTLELVVTDSIHQDYTTQNLIR
MAVAGLVLVALLAILVENWHSHTALNKEASADVA
EPSWSQQMCQPGLTFARTPSVCK*TPGGEGREE
PGLWSPTKLLEGHKREKLTKKLESTFFFF*DRV
WLCHPGWSAVEQSRLIEPLGFK*FLCLSLPSSWN
YRHIPLHPANFCIFSRDGVSLCWPGWSRTPDLR
*STHLGLPKC*DYRHEPPRLARCMFKPIKWCFL
MQD*SICTHLSAHKLWFSILSVF*FFISCLISSF
IVLLFFYLFYLFLFLFLFETESHSVAQ
Sequence NM_133279
TCCACCCAAGAGCAACCTGGAACTAAGTTATTCGGCAACGAACTGTTCCACTTTGTTGTGAGGCAATAGATGTGGAAATTCCCTGACGAGGGGCTCTGTCCTCATACTTCCTGCGGAGCTTATTGTCGTAAGAATATCTGTCATCCTGCTAATGTGCATTGAAAGGAGAGCAACGGGGCTGAGGCCGTGTCAGCACGATG
GACCCCAAACAGACCACCCTCCTGTGTCTTGTGCTCTGTCTGGGCCAGAGGATTCAGGCACAGGAAGGGGACTTTCCCATGCCTTTCATATCTGCCAAAT
CGAGTCCTGTGATTCCCTTGGATGGATCTGTGAAAATCCAGTGCCAGGCCATTCGTGAAGCTTACCTGACCCAGCTGATGATCATAAAAAACTCCACGTA
CCGAGAGATAGGCAGAAGACTGAAGTTTTGGAATGAGACTGATCCTGAGTTCGTCATTGACCACATGGACGCAAACAAGGCAGGGCGCTATCAGTGCCAA
TATAGGATAGGGCACTACAGATTCCGGTACAGTGACACCCTGGAGCTGGTAGTGACAGGCTTGTATGGCAAACCCTTCCTCTCTGCAGATCGGGGTCTGG
TGTTGATGCCAGGAGAGAATATTTCCCTCACGTGCAGCTCAGCACACATCCCATTTGATAGATTTTCACTGGCCAAGGAGGGAGAACTTTCTCTGCCACA
GCACCAAAGTGGGGAACACCCGGCCAACTTCTCTTTGGGTCCTGTGGACCTCAATGTCTCAGGGATCTACAGGTGCTACGGTTGGTACAACAGGAGCCCC
TACCTGTGGTCCTTCCCCAGTAATGCCTTGGAGCTTGTGGTCACAGGTAGGTACCGCCCAGTCCAGCCCTGTGTCTGGGTTGGCTGTCCAGGGCCTTGCC
ACCGGGCAGGAATATGAAGACGTGCACTGAGAGTGAAGTGAAGAGAGGCAAAGGCTCTCACTCCAGGACAGTGGAGAGAGAAAGGCTTCCCCACCACACT
TTCCGCTTTCACTTCCTCGCTAGAGTTCTCCAGACAGGGTTCATTGAAAACTTAGTCTGTGGAGAACAGAAGGGCTAACTCAGTTTGTTTCATTTTATTT
ATTTCATTTTATTTTCCGGGATAGAGTCTTGCTCTTTCGCCAAGGCTGGAGTGCAGTGGCACGATCTCGACTCACTGCAACCTTCGCCTCCCAGGTTCAA
GCAATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACCACACAGACAGGGTTTCACCATGTTGGCCAGGCTGGTCTCGAACTCCCGACCTCAGGTGATCC
ACCTGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCTGGCCAGGCTGCACACATTCTTATTAGGATTCCACCTTGTTCTGGTGT
TGTAGAGATGTGATTAGGTATTTAGTGAATTCACCAAGTGAGGAGAGAATGAAAAGAAAACACAACCTGCCTGGCCGGGCGTGGTGGCGTGAGCCTGTCG
TCCCAGCTACTCAGGAGGCTGAGGCAGGAGAATCACTTGAACCCAGGAGGCAGCTGTTGCAGTGAGCCAAGATCACGCCATTGCACTCCAGCCTGGGTGA
CAGAACGAGACTCCACCTCAAGAAAAAAAAAAAAAA
- refseq_FCAR.F4 (forward primer)
- refseq_FCAR.R4 (reverse primer)
- Single exon skipping
Frame 0
STQEQPGTKLFGNELFHFVVRQ*MWKFPDEGLCPHTSCGAYCRKNICHPANVH*KESNGAEAVSARW
TPNRPPSCVLCSVWARGFRHRKGTFPCLSYLPN
RVL*FPWMDL*KSSARPFVKLT*PS**S*KTPR
TER*AED*SFGMRLILSSSLTTWTQTRQGAISAN
IG*GTTDSGTVTPWSW**QACMANPSSLQIGVW
C*CQERIFPSRAAQHTSHLIDFHWPRRENFLCH
STKVGNTRPTSLWVLWTSMSQGSTGATVGTTGAP
TCGPSPVMPWSLWSQVGTAQSSPVSGLAVQGLA
TGQEYEDVH*E*SEERQRLSLQDSGERKASPPH
FPLSLPR*SSPDRVH*KLSLWRTEGLTQFVSFYL
FHFIFRDRVLLFRQGWSAVARSRLTATFASQVQ
AILLPQPPE*LGPHRQGFTMLARLVSNSRPQVI
HLPRPPKVLGLQA*ATAPGQAAHILIRIPPCSGV
VEM*LGI**IHQVRRE*KENTTCLAGRGGVSLS
SQLLRRLRQENHLNPGGSCCSEPRSRHCTPAWV
TERDSTSRKKKK
Frame 1
PPKSNLELSYSATNCSTLL*GNRCGNSLTRGSVLILPAELIVVRISVILLMCIERRATGLRPCQHD
GPQTDHPPVSCALSGPEDSGTGRGLSHAFHICQI
ESCDSLGWICENPVPGHS*SLPDPADDHKKLHV
PRDRQKTEVLE*D*S*VRH*PHGRKQGRALSVP
I*DRALQIPVQ*HPGAGSDRLVWQTLPLCRSGSG
VDARREYFPHVQLSTHPI**IFTGQGGRTFSAT
APKWGTPGQLLFGSCGPQCLRDLQVLRLVQQEP
LPVVLPQ*CLGACGHR*VPPSPALCLGWLSRALP
PGRNMKTCTESEVKRGKGSHSRTVERERLPHHT
FRFHFLARVLQTGFIENLVCGEQKG*LSLFHFI
YFILFSGIESCSFAKAGVQWHDLDSLQPSPPRFK
QFSCLSLLSSWDHTDRVSPCWPGWSRTPDLR*S
TCLGLPKCWDYRREPPRLARLHTFLLGFHLVLV
L*RCD*VFSEFTK*GENEKKTQPAWPGVVA*ACR
PSYSGG*GRRIT*TQEAAVAVSQDHAIALQPG*
QNETPPQEKKKK
Frame 2
HPRATWN*VIRQRTVPLCCEAIDVEIP*RGALSSYFLRSLLS*EYLSSC*CALKGEQRG*GRVSTM
DPKQTTLLCLVLCLGQRIQAQEGDFPMPFISAK
SSPVIPLDGSVKIQCQAIREAYLTQLMIIKNSTY
REIGRRLKFWNETDPEFVIDHMDANKAGRYQCQ
YRIGHYRFRYSDTLELVVTGLYGKPFLSADRGL
VLMPGENISLTCSSAHIPFDRFSLAKEGELSLPQ
HQSGEHPANFSLGPVDLNVSGIYRCYGWYNRSP
YLWSFPSNALELVVTGRYRPVQPCVWVGCPGPC
HRAGI*RRALRVK*REAKALTPGQWREKGFPTTL
SAFTSSLEFSRQGSLKT*SVENRRANSVCFILF
ISFYFPG*SLALSPRLECSGTISTHCNLRLPGS
SNSPASAS*VAGTTQTGFHHVGQAGLELPTSGDP
PASASQSAGITGVSHRAWPGCTHSY*DSTLFWC
CRDVIRYLVNSPSEERMKRKHNLPGRAWWREPV
VPATQEAEAGESLEPRRQLLQ*AKITPLHSSLGD
RTRLHLKKKKK
Sequence NM_133269
TCCACCCAAGAGCAACCTGGAACTAAGTTATTCGGCAACGAACTGTTCCACTTTGTTGTGAGGCAATAGATGTGGAAATTCCCTGACGAGGGGCTCTGTCCTCATACTTCCTGCGGAGCTTATTGTCGTAAGAATATCTGTCATCCTGCTAATGTGCATTGAAAGGAGAGCAACGGGGCTGAGGCCGTGTCAGCACGATG
GACCCCAAACAGACCACCCTCCTGTGTCTTGTGCTCTGTCTGGGCCAGAGGATTCAGGCACAGGAAGGGGACTTTCCCATGCCTTTCATATCTGCCAAAT
CGAGTCCTGTGATTCCCTTGGATGGATCTGTGAAAATCCAGTGCCAGGCCATTCGTGAAGCTTACCTGACCCAGCTGATGATCATAAAAAACTCCACGTA
CCGAGAGATAGGCAGAAGACTGAAGTTTTGGAATGAGACTGATCCTGAGTTCGTCATTGACCACATGGACGCAAACAAGGCAGGGCGCTATCAGTGCCAA
TATAGGATAGGGCACTACAGATTCCGGTACAGTGACACCCTGGAGCTGGTAGTGACAGGCTTGTATGGCAAACCCTTCCTCTCTGCAGATCGGGGTCTGG
TGTTGATGCCAGGAGAGAATATTTCCCTCACGTGCAGCTCAGCACACATCCCATTTGATAGATTTTCACTGGCCAAGGAGGGAGAACTTTCTCTGCCACA
GCACCAAAGTGGGGAACACCCGGCCAACTTCTCTTTGGGTCCTGTGGACCTCAATGTCTCAGGGATCTACAGGTGCTACGACTCCATCCACCAAGATTAC
ACGACGCAGAACTTGATCCGCATGGCCGTGGCAGGACTGGTCCTCGTGGCTCTCTTGGCCATACTGGTTGAAAATTGGCACAGCCATACGGCACTGAACA
AGGAAGCCTCGGCAGATGTGGCTGAACCGAGCTGGAGCCAACAGATGTGTCAGCCAGGATTGACCTTTGCACGAACACCAAGTGTCTGCAAGTAAACACC
TGGAGGTGAAGGCAGAGAGGAGCCAGGACTGTGGAGTCCGACAAAGCTACTTGAAGGACACAAGAGAGAAAAGCTCACTAAGAAGCTTGAATCTACTTTT
TTTTTTTTTTGAGACAGAGTCTGGCTCTGTCACCCAGGCTGGAGTGCAGTGGAGCAATCTCGGCTCATTGAACCTCTTGGGTTCAAGTGATTCTTGTGCC
TCAGCCTCCCAAGTAGCTGGAATTACAGGCACATACCACTGCACCCAGCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTCACTGTGTTGGCCAGGCTG
GTCTCGAACTCCTGACCTCAGGTGATCCACCCACCTTGGCCTCCCAAAGTGCTGAGATTATAGGCATGAGCCACCACGCCTGGCCAGATGCATGTTCAAA
CCAATCAAATGGTGTTTTCTTATGCAGGACTGATCGATTTGCACCCACCTTTCTGCACATAAGTTATGGTTTTCCATCTTATCTGTCTTCTGATTTTTTA
TATCCTGTTTAATTTCTTCCTTCATTGTTCTTCTCTTTTTTTATTTATTTTATTTATTTTTATTTTTATTTTTATTTGAGACAGAGTCTCACTCTGTTGC
CCAGG
- refseq_FCAR.F4 (forward primer)
- refseq_FCAR.R4 (reverse primer)
- Single exon skipping
Frame 0
STQEQPGTKLFGNELFHFVVRQ*MWKFPDEGLCPHTSCGAYCRKNICHPANVH*KESNGAEAVSARW
TPNRPPSCVLCSVWARGFRHRKGTFPCLSYLPN
RVL*FPWMDL*KSSARPFVKLT*PS**S*KTPR
TER*AED*SFGMRLILSSSLTTWTQTRQGAISAN
IG*GTTDSGTVTPWSW**QACMANPSSLQIGVW
C*CQERIFPSRAAQHTSHLIDFHWPRRENFLCH
STKVGNTRPTSLWVLWTSMSQGSTGATTPSTKIT
RRRT*SAWPWQDWSSWLSWPYWLKIGTAIRH*T
RKPRQMWLNRAGANRCVSQD*PLHEHQVSASKH
LEVKAERSQDCGVRQSYLKDTREKSSLRSLNLLF
FFFETESGSVTQAGVQWSNLGSLNLLGSSDSCA
SASQVAGITGTYHCTQLIFVFLVEMGFHCVGQA
GLELLTSGDPPTLASQSAEIIGMSHHAWPDACSN
QSNGVFLCRTDRFAPTFLHISYGFPSYLSSDFL
YPV*FLPSLFFSFFIYFIYFYFYFYLRQSLTLL
PR
Frame 1
PPKSNLELSYSATNCSTLL*GNRCGNSLTRGSVLILPAELIVVRISVILLMCIERRATGLRPCQHD
GPQTDHPPVSCALSGPEDSGTGRGLSHAFHICQI
ESCDSLGWICENPVPGHS*SLPDPADDHKKLHV
PRDRQKTEVLE*D*S*VRH*PHGRKQGRALSVP
I*DRALQIPVQ*HPGAGSDRLVWQTLPLCRSGSG
VDARREYFPHVQLSTHPI**IFTGQGGRTFSAT
APKWGTPGQLLFGSCGPQCLRDLQVLRLHPPRL
HDAELDPHGRGRTGPRGSLGHTG*KLAQPYGTEQ
GSLGRCG*TELEPTDVSARIDLCTNTKCLQVNT
WR*RQRGARTVESDKAT*RTQERKAH*EA*IYF
FFFLRQSLALSPRLECSGAISAH*TSWVQVILVP
QPPK*LELQAHTTAPS*FLYF**RWGFTVLARL
VSNS*PQVIHPPWPPKVLRL*A*ATTPGQMHVQ
TNQMVFSYAGLIDLHPPFCT*VMVFHLICLLIFY
ILFNFFLHCSSLFLFILFIFIFIFI*DRVSLCC
P
Frame 2
HPRATWN*VIRQRTVPLCCEAIDVEIP*RGALSSYFLRSLLS*EYLSSC*CALKGEQRG*GRVSTM
DPKQTTLLCLVLCLGQRIQAQEGDFPMPFISAK
SSPVIPLDGSVKIQCQAIREAYLTQLMIIKNSTY
REIGRRLKFWNETDPEFVIDHMDANKAGRYQCQ
YRIGHYRFRYSDTLELVVTGLYGKPFLSADRGL
VLMPGENISLTCSSAHIPFDRFSLAKEGELSLPQ
HQSGEHPANFSLGPVDLNVSGIYRCYDSIHQDY
TTQNLIRMAVAGLVLVALLAILVENWHSHTALN
KEASADVAEPSWSQQMCQPGLTFARTPSVCK*TP
GGEGREEPGLWSPTKLLEGHKREKLTKKLESTF
FFF*DRVWLCHPGWSAVEQSRLIEPLGFK*FLC
LSLPSSWNYRHIPLHPANFCIFSRDGVSLCWPGW
SRTPDLR*STHLGLPKC*DYRHEPPRLARCMFK
PIKWCFLMQD*SICTHLSAHKLWFSILSVF*FF
ISCLISSFIVLLFFYLFYLFLFLFLFETESHSVA
Q
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