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Sequences

Sequence NM_012174

GCGGCGGACACTTCCCTGGGCGGGACTGTCTCGTGGCACCCGGTGGAACCGAGGAGAACGTGGAGCGCCGGGAGCGGCGAATATGGACGACTACAGCCTG
GATGAGTTCCGTCG
GCGCTGGCAGGAGGAGCTGGCGCAGGCCCAGGCGCCGAAGAAGCGGCGACGGCCCGAGGCTGCCGAGAGGCGGGCTCGGCGGCCGG
AGAATGAAATGAATGATGTGCCTTTCTTTGATATCCAACTGCCTTACGAATTGGCAATCAATATATTTCAGTATCTGGACAGGAAAGAACTAGGAAGATG
TGCACAGGTGAGCAAGACGTGGAAGGTGATTGCAGAGGATGAGGTGCTGTGGTACAGGCTGTGCCAGCAGGAAGGGCACCTTCCGGATAGCAGCATCTCT
GACTATTCTTGCTGGAAGCTCATCTTCCAAGAGTGCCGAGCCAAGGAACACATGTTACGAACCAACTGGAAGAATCGCAAAGGTGCCGTGAGCGAGCTGG
AGCATGTTCCTGACACAGTTTTGTGTGATGTGCATTCTCACGATGGTGTGGTCATTGCGGGATATACATCAGGGGATGTGAGAGTGTGGGACACCCGCAC
CTGGGACTACGTAGCCCCCTTCCTGGAATCAGAGGACGAGGAGGATGAGCCTGGAATGCAGCCAAATGTCTCCTTTGTGAGGATAAACAGCTCGTTGGCA
GTAGCAGCTTATGAGGATGGGTTTCTTAATATTTGGGATTTAAGGACCGGAAAGTACCCTGTTCATCGTTTTGAGCACGATGCAAGAATACAGGCACTAG
CCCTCAGCCAGGACGATGCAACCGTGGCCACAGCTTCTGCTTTTGATGTCGTGATGTTATCCCCCAATGAGGAGGGGTACTGGCAGATAGCTGCGGAATT
TGAAGTTCCGAAACTGGTTCAGTACCTTGAAATAGTTCCAGAAACCAGAAGGTACCCTGTGGCAGTAGCCGCTGCTGGAGATCTGATGTACCTGCTCAAA
GCCGAAGACTCCGCCAGAACCCTCCTTTACGCCCACGGCCCGCCTGTCACATGTCTAGACGTCTCGGCCAACCAAGTTGCTTTTGGTGTACAGGGTCTGG
GATGGGTGTACGAAGGAAGCAAGATCCTGGTGTATAGCCTGGAAGCAGGACGCCGCCTCTTGAAGCTGGGTAACGTTCTCCGTGACTTCACGTGTGTCAA
CCTCAGCGACAGCCCTCCCAACCTCATGGTCAGTGGCAACATGGACGGGAGGGTGAGGATCCACGACCTCCGCAGTGGTAACATCGCCCTGTCGCTCTCC
GCCCATCAGCTCAGGGTCTCTGCTGTGCAGATGGATGACTGGAAGATCGTCAGTGGAGGCGAGGAAGGCCTGGTGTCCGTGTGGGATTATCGGATGAACC
AGAAGCTGTGGGAGGTGTATTCCGGGCACCCGGTGCAGCACATCTCATTCAGCAGCCACAGCCTCATCACGGCCAACGTGCCTTACCAGACGGTAATGCG
AAACGCCGACCTGGACAGCTTCACTACTCACAGGAGACACCGGGGGCTGATCCGCGCCTATGAGTTTGCGGTGGACCAGCTGGCCTTCCAGAGCCCTCTC
CCTGTCTGCCGTTCATCCTGTGACGCCATGGCCACTCACTACTACGACCTCGCACTGGCCTTTCCCTATAACCATGTTTAGGGATGTGCCTCAGTTGGGA
GCAAGGAGAAAAATGGGAAGAACCAGTTTTATCCATCTTAAAACGCCAGGCACCTCTTCACAGGTGGTAAACATTTAGGGGAAGAAAGCAGCCCAGGGTG
CCATGCCTGACAGCACGCATCTCCCTGACCCCTGCACTTCCCCCAGCGCCTGGGGCAAGCTGGCGTGTGCCAGGGCTCGAGTCCCACGTGCTGCCAACTC
AAACATAGCCTCCTTCCCCACCCAGCTGGCCACCCTGGCCTCAGCTCCCTCAGGACGCCTCAGGGATCTCGCTGCGCGGTCCTATACGGTCCCTGCTTAG
CCAGCTTCTGTGTGTCCGCCCTCCCAGCTCCAGCCCTGCAGGCCGTCTTTTCCGTCTCCAGCCCCTTACCTCTTTTCCTCCGAGGGCCTTTGGATGTGCT
TGTTCCTGGCCTCCAAGGCAATAACCTCCATGTCCTTTTCCCTGTATTTACTTGGGGTGCTCTTCTGTGACACTTGCCACCACCACCTTCCAGTAGCAAG
CTGGTAGAGCTGTACTTTTTCTTCACAGCTTTTACCATGGTTTATAATTATATACTTATACAGTTTAGTCATCAATGACTATTTTGCTTACTATTGTGTC
TATGATATGTGATACAAAGCAAATATTTTCTAAATATTCATTGACTAAACAGCCATCAAGATGAAACAGGACAGGAGAGAACTGTTCAGTGTGATACAGA
GCTGCCATTTCCAAAGTATTCTGGAAGCAAGCCATTGCCTTACTCTTGATTTTCAACAGTTCTAAACAGCAACAGCATCCACCTAATATACCTTCAGAAT
ATCAAAGCGAAAACTGATAGAACTACAAAGAGAAATAAAATCCATTCTCACGGCAGGCAAATCAGTGAGAAAAATGTAAAGATCTGGAGGATTTACTTAG
TATCGCTAACAAGCTGGATCCAGTAGATGCGTATGGAATTTCAAATACACATAGACGATGCACAAAAAACCACCCTGTACGAAGGCCACAAAGAACAGCT
CAGATGCACAAAGATTCACACCATACAGGCCAAATTATAGAATCCCCCAACCCCAAGACACTGCACCCTATGGGCTATACCCTCAGGCAGGGCCCTGCTT
TTCTAGGAATTGACTCCAATGCAGAATAGTGAGAAATCAACAAAAAGCAAAATAAGACAATTCCAGACACTCCTGAATTTACTTGGATTTAAAGAGGTTA
ATAAAAACAAACTATAAACTCTCTAGACTGGGCTGACAGTAAAAGCACAGAATACAAAACCCAGGGGATGGGGCTGGGAGGGGCTGTGAGGGGCTGGCAC
CTCAGCTCAGAAAAGGTAGCAGCAATCCCAAAGCAGCAGAAGGCAGGGGTTAAATCAGGATAAAAACAGACATCAGTGGAATCGAAAACCAATAGTTAGT
TAATATAAAGCCAGAAGGTTTTGTTTTTTTTCAGGTGGGGTCTCGCTCTGTCACCTAGACTAGACTGGAGTGCAATGGCCTGATCTCGGCTCACTGCAAC
CTCTGCCTCCCGAGTTCAAGCAATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGTCCGCTACCACAACTGGCTAATTTTTACATTTTTAG
TAGAGACGGGGGATATTGCTATGTTGGCCAGGCTGGTCTCAAACTTCTGACCTCAAATGATTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTG
TAAGTCACTGTACCCAGCCCAAAAGCAGTTTAAAAAAAAAAAAGACAAAATATACAACTCTTGATAAAACATAAAGGTACAGTGGTCTATGAGGAAGAGA
AAAGGTACCTGAGGATGCAGAAGTACCTACCACATGGGAACCGTTTGTCCACACTCATTCCAGAGAAAACCGAGTCCTCTCAGTTGCACACGTGTACGTA
TCAGTGGGAAGTGCTTGCCATTACTCCAAAGCCTAGAACCTTCACGTCATGAAGGTTCTGGAAGGTTTTTCAGATTGCTTAAGATACGCAGCCATTCCAT
ATTCATCTCCAACTACACAGGGGAACGGAGCAGATAGAGCTGCGACTGGGAAGCGTCACCTTCCCGTCCAGAGCGCTTTCTTTCAGACCCTGCCTACCTG
CAGGCAGATGGACCGGAGGGTTTTCTGCTTCCTTTCAACCAGATAACTTCCTAAGTGGAGATGGCCTGTAGGTAGCAAATGCAGGATTTTGTTTACTTTC
ATCATGTCATGTGGTGGTCAGACTGCTCGCTGGTGGCCTCGCTTTAGAAGGTTTTCATCAAGCCCCGCCCTTTCTCTCTCATAGTCTTAATGCGTCTGGA
CCACTGGGGAAAATATTTTTCTTTTCAAAAAGCAGCCCCTTCAGTCTGCGTTCCCAGTTCATTTTGCAGGCGCATTCGCCCTTCCTAGTTTGCAGCAATT
AAAGATAACGGAGTGTAAAGTCATTAGACCCAGAATTAGAGCAGCCAGCAGCTGGCCCAGTGTAAAAATGTCCCAAGTGTAGCCCCTCCCACCGCAGAGC
CTGTTTCTAACGTACCTGCCATTCGTGGAAGTCGCCTGGGTTTTGTAAAGGAGAATTTTTTGGACTCAAGCTGTCATCTTCAAGCACGCCAACTTCAGGC
AGTGTTTTCTACTGCAAACCTCCACATCCTGGAATCAATCACACTGACAGGGAAAGGATAAATGCTCCTTTATAAGCCAACATGTATCCAAATTAAGCCC
CCTTACCTTTATCCAGACCCTTTCACAAACAAGTTCTGAGTTTTTAGCCAATTGATCCTCTACTTTCCTTCTTTAGTCCCTCTCAGGGACGCCTCCATCC
CCGGCTCCCCTAAAGGTAGAAGATACTCGAGCATGCTGCCAGGGGAGCTGGATGGTGAAACACCTGAAGATCTCATATCGCCTCTCAAAACACTGTCGGA
CTTGCTTTCTGCTTTATGATGTCCGTAGTTGTTCTAATATTAAATGCTTTGAATTTC
  • refseq_FBXW8.F1 (forward primer)
  • refseq_FBXW8.R1 (reverse primer)
  • Alternative donor

Frame 0

AADTSLGGTVSWHPVEPRRTWSAGSGEYGRLQP
G*VPS
ALAGGAGAGPGAEEAATARGCREAGSAAG
E*NE*CAFL*YPTALRIGNQYISVSGQERTRKM
CTGEQDVEGDCRG*GAVVQAVPAGRAPSG*QHL
*LFLLEAHLPRVPSQGTHVTNQLEESQRCRERAG
ACS*HSFV*CAFSRWCGHCGIYIRGCESVGHPH
LGLRSPLPGIRGRGG*AWNAAKCLLCEDKQLVG
SSSL*GWVS*YLGFKDRKVPCSSF*ARCKNTGTS
PQPGRCNRGHSFCF*CRDVIPQ*GGVLADSCGI
*SSETGSVP*NSSRNQKVPCGSSRCWRSDVPAQ
SRRLRQNPPLRPRPACHMSRRLGQPSCFWCTGSG
MGVRRKQDPGV*PGSRTPPLEAG*RSP*LHVCQ
PQRQPSQPHGQWQHGREGEDPRPPQW*HRPVAL
RPSAQGLCCADG*LEDRQWRRGRPGVRVGLSDEP
EAVGGVFRAPGAAHLIQQPQPHHGQRALPDGNA
KRRPGQLHYSQETPGADPRL*VCGGPAGLPEPS
PCLPFIL*RHGHSLLRPRTGLSL*PCLGMCLSWE
QGEKWEEPVLSILKRQAPLHRW*TFRGRKQPRV
PCLTARISLTPALPPAPGASWRVPGLESHVLPT
QT*PPSPPSWPPWPQLPQDASGISLRGPIRSLLS
QLLCVRPPSSSPAGRLFRLQPLTSFPPRAFGCA
CSWPPRQ*PPCPFPCIYLGCSSVTLATTTFQ*Q
AGRAVLFLHSFYHGL*LYTYTV*SSMTILLTIVS
MICDTKQIFSKYSLTKQPSR*NRTGENCSV*YR
AAISKVFWKQAIALLLIFNSSKQQQHPPNIPSE
YQSEN**NYKEK*NPFSRQANQ*EKCKDLEDLLS
IANKLDPVDAYGISNTHRRCTKNHPVRRPQRTA
QMHKDSHHTGQIIESPNPKTLHPMGYTLRQGPA
FLGIDSNAE**EINKKQNKTIPDTPEFTWI*RG*
*KQTINSLDWADSKSTEYKTQGMGLGGAVRGWH
LSSEKVAAIPKQQKAGVKSG*KQTSVESKTNS*
LI*SQKVLFFFRWGLALSPRLDWSAMA*SRLTAT
SASRVQAILLPQPPE*LGLQASATTTG*FLHF*
*RRGILLCWPGWSQTSDLK*LPASASQSAGITG
VSHCTQPKSSLKKKKTKYTTLDKT*RYSGL*GRE
KVPEDAEVPTTWEPFVHTHSRENRVLSVAHVYV
SVGSACHYSKA*NLHVMKVLEGFSDCLRYAAIP
YSSPTTQGNGADRAATGKRHLPVQSAFFQTLPTC
RQMDRRVFCFLSTR*LPKWRWPVGSKCRILFTF
IMSCGGQTARWWPRFRRFSSSPALSLS*S*CVW
TTGENIFLFKKQPLQSAFPVHFAGAFALPSLQQL
KITECKVIRPRIRAASSWPSVKMSQV*PLPPQS
LFLTYLPFVEVAWVL*RRIFWTQAVIFKHANFR
QCFLLQTSTSWNQSH*QGKDKCSFISQHVSKLSP
LTFIQTLSQTSSEFLAN*SSTFLL*SLSGTPPS
PAPLKVEDTRACCQGSWMVKHLKISYRLSKHCR
TCFLLYDVRSCSNIKCFEF

Frame 1

RRTLPWAGLSRGTRWNRGERGAPGAANMDDYSL
DEFRR
RWQEELAQAQAPKKRRRPEAAERRARRP
ENEMNDVPFFDIQLPYELAINIFQYLDRKELGRC
AQVSKTWKVIAEDEVLWYRLCQQEGHLPDSSIS
DYSCWKLIFQECRAKEHMLRTNWKNRKGAVSEL
EHVPDTVLCDVHSHDGVVIAGYTSGDVRVWDTRT
WDYVAPFLESEDEEDEPGMQPNVSFVRINSSLA
VAAYEDGFLNIWDLRTGKYPVHRFEHDARIQAL
ALSQDDATVATASAFDVVMLSPNEEGYWQIAAEF
EVPKLVQYLEIVPETRRYPVAVAAAGDLMYLLK
AEDSARTLLYAHGPPVTCLDVSANQVAFGVQGL
GWVYEGSKILVYSLEAGRRLLKLGNVLRDFTCVN
LSDSPPNLMVSGNMDGRVRIHDLRSGNIALSLS
AHQLRVSAVQMDDWKIVSGGEEGLVSVWDYRMN
QKLWEVYSGHPVQHISFSSHSLITANVPYQTVMR
NADLDSFTTHRRHRGLIRAYEFAVDQLAFQSPL
PVCRSSCDAMATHYYDLALAFPYNHV*GCASVG
SKEKNGKNQFYPS*NARHLFTGGKHLGEESSPGC
HA*QHASP*PLHFPQRLGQAGVCQGSSPTCCQL
KHSLLPHPAGHPGLSSLRTPQGSRCAVLYGPCL
ASFCVSALPAPALQAVFSVSSPLPLFLRGPLDVL
VPGLQGNNLHVLFPVFTWGALL*HLPPPPSSSK
LVELYFFFTAFTMVYNYILIQFSHQ*LFCLLLC
L*YVIQSKYFLNIH*LNSHQDETGQERTVQCDTE
LPFPKYSGSKPLPYS*FSTVLNSNSIHLIYLQN
IKAKTDRTTKRNKIHSHGRQISEKNVKIWRIYL
VSLTSWIQ*MRMEFQIHIDDAQKTTLYEGHKEQL
RCTKIHTIQAKL*NPPTPRHCTLWAIPSGRALL
F*ELTPMQNSEKSTKSKIRQFQTLLNLLGFKEV
NKNKL*TL*TGLTVKAQNTKPRGWGWEGL*GAGT
SAQKR*QQSQSSRRQGLNQDKNRHQWNRKPIVS
*YKARRFCFFSGGVSLCHLD*TGVQWPDLGSLQ
PLPPEFKQFSCLSLLSSWDYRRPLPQLANFYIFS
RDGGYCYVGQAGLKLLTSNDCLPQPPKVLGLQV
*VTVPSPKAV*KKKRQNIQLLIKHKGTVVYEEE
KRYLRMQKYLPHGNRLSTLIPEKTESSQLHTCTY
QWEVLAITPKPRTFTS*RFWKVFQIA*DTQPFH
IHLQLHRGTEQIELRLGSVTFPSRALSFRPCLP
AGRWTGGFSASFQPDNFLSGDGL*VANAGFCLLS
SCHVVVRLLAGGLALEGFHQAPPFLSHSLNASG
PLGKIFFFSKSSPFSLRSQFILQAHSPFLVCSN
*R*RSVKSLDPELEQPAAGPV*KCPKCSPSHRRA
CF*RTCHSWKSPGFCKGEFFGLKLSSSSTPTSG
SVFYCKPPHPGINHTDRERINAPL*ANMYPN*A
PLPLSRPFHKQVLSF*PIDPLLSFFSPSQGRLHP
RLP*R*KILEHAARGAGW*NT*RSHIASQNTVG
LAFCFMMSVVVLILNALNF

Frame 2

GGHFPGRDCLVAPGGTEENVERRERRIWTTTAW
MSSV
GAGRRSWRRPRRRRSGDGPRLPRGGLGGR
RMK*MMCLSLISNCLTNWQSIYFSIWTGKN*ED
VHR*ARRGR*LQRMRCCGTGCASRKGTFRIAASL
TILAGSSSSKSAEPRNTCYEPTGRIAKVP*ASW
SMFLTQFCVMCILTMVWSLRDIHQGM*ECGTPA
PGTT*PPSWNQRTRRMSLECSQMSPL*G*TARWQ
*QLMRMGFLIFGI*GPESTLFIVLSTMQEYRH*
PSARTMQPWPQLLLLMS*CYPPMRRGTGR*LRN
LKFRNWFSTLK*FQKPEGTLWQ*PLLEI*CTCSK
PKTPPEPSFTPTARLSHV*TSRPTKLLLVYRVW
DGCTKEARSWCIAWKQDAAS*SWVTFSVTSRVS
TSATALPTSWSVATWTGG*GSTTSAVVTSPCRSP
PISSGSLLCRWMTGRSSVEARKAWCPCGIIG*T
RSCGRCIPGTRCSTSHSAATASSRPTCLTRR*C
ETPTWTASLLTGDTGG*SAPMSLRWTSWPSRALS
LSAVHPVTPWPLTTTTSHWPFPITMFRDVPQLG
ARRKMGRTSFIHLKTPGTSSQVVNI*GKKAAQG
AMPDSTHLPDPCTSPSAWGKLACARARVPRAANS
NIASFPTQLATLASAPSGRLRDLAARSYTVPA*
PASVCPPSQLQPCRPSFPSPAPYLFSSEGLWMC
LFLASKAITSMSFSLYLLGVLFCDTCHHHLPVAS
W*SCTFSSQLLPWFIIIYLYSLVINDYFAYYCV
YDM*YKANIF*IFID*TAIKMKQDRRELFSVIQ
SCHFQSILEASHCLTLDFQQF*TATAST*YTFRI
SKRKLIELQREIKSILTAGKSVRKM*RSGGFT*
YR*QAGSSRCVWNFKYT*TMHKKPPCTKATKNS
SDAQRFTPYRPNYRIPQPQDTAPYGLYPQAGPCF
SRN*LQCRIVRNQQKAK*DNSRHS*IYLDLKRL
IKTNYKLSRLG*Q*KHRIQNPGDGAGRGCEGLA
PQLRKGSSNPKAAEGRG*IRIKTDISGIENQ*LV
NIKPEGFVFFQVGSRSVT*TRLECNGLISAHCN
LCLPSSSNSPASAS*VAGITGVRYHNWLIFTFL
VETGDIAMLARLVSNF*PQMIACLSLPKCWDYRC
KSLYPAQKQFKKKKDKIYNS**NIKVQWSMRKR
KGT*GCRSTYHMGTVCPHSFQRKPSPLSCTRVR
ISGKCLPLLQSLEPSRHEGSGRFFRLLKIRSHSI
FISNYTGERSR*SCDWEASPSRPERFLSDPAYL
QADGPEGFLLPFNQITS*VEMACR*QMQDFVYF
HHVMWWSDCSLVASL*KVFIKPRPFSLIVLMRLD
HWGKYFSFQKAAPSVCVPSSFCRRIRPS*FAAI
KDNGV*SH*TQN*SSQQLAQCKNVPSVAPPTAE
PVSNVPAIRGSRLGFVKENFLDSSCHLQARQLQA
VFSTANLHILESITLTGKG*MLLYKPTCIQIKP
PYLYPDPFTNKF*VFSQLILYFPSLVPLRDASI
PGSPKGRRYSSMLPGELDGETPEDLISPLKTLSD
LLSAL*CP*LF*Y*ML*I

Sequence NM_153348

GCGGCGGACACTTCCCTGGGCGGGACTGTCTCGTGGCACCCGGTGGAACCGAGGAGAACGTGGAGCGCCGGGAGCGGCGAATATGGACGACTACAGCCTG
GATGAGTTCCGTCG
GCGCTGGCAGGAGGAGCTGGCGCAGGCCCAGGCGCCGAAGAAGCGGCGACGGCCCGAGGCTGCCGAGAGGCGGGCTCGGCGGCCGG
AGGTGGGCTCCGGGCGCGGCGAACAGGCCTCGGGGGACCCGGCGCTGGCCCAGCGTCTCCTGGAGGGCGCGGGGAGGCCCCCGGCGGCGCGGGCGACTCG
GGCCGAGGGGCAGGACGTAGCGAGCCGCTCACGTTCTCCTCTGGCCCGCGAGGGCGCCGGGGGCGGGGAGCAGCTGGTGGACCAGCTCATCCGCGACCTG

AATGAAATGAATGATGTGCCTTTCTTTGATATCCAACTGCCTTACGAATTGGCAATCAATATATTTCAGTATCTGGACAGGAAAGAACTAGGAAGATGTG
CACAGGTGAGCAAGACGTGGAAGGTGATTGCAGAGGATGAGGTGCTGTGGTACAGGCTGTGCCAGCAGGAAGGGCACCTTCCGGATAGCAGCATCTCTGA
CTATTCTTGCTGGAAGCTCATCTTCCAAGAGTGCCGAGCCAAGGAACACATGTTACGAACCAACTGGAAGAATCGCAAAGGTGCCGTGAGCGAGCTGGAG
CATGTTCCTGACACAGTTTTGTGTGATGTGCATTCTCACGATGGTGTGGTCATTGCGGGATATACATCAGGGGATGTGAGAGTGTGGGACACCCGCACCT
GGGACTACGTAGCCCCCTTCCTGGAATCAGAGGACGAGGAGGATGAGCCTGGAATGCAGCCAAATGTCTCCTTTGTGAGGATAAACAGCTCGTTGGCAGT
AGCAGCTTATGAGGATGGGTTTCTTAATATTTGGGATTTAAGGACCGGAAAGTACCCTGTTCATCGTTTTGAGCACGATGCAAGAATACAGGCACTAGCC
CTCAGCCAGGACGATGCAACCGTGGCCACAGCTTCTGCTTTTGATGTCGTGATGTTATCCCCCAATGAGGAGGGGTACTGGCAGATAGCTGCGGAATTTG
AAGTTCCGAAACTGGTTCAGTACCTTGAAATAGTTCCAGAAACCAGAAGGTACCCTGTGGCAGTAGCCGCTGCTGGAGATCTGATGTACCTGCTCAAAGC
CGAAGACTCCGCCAGAACCCTCCTTTACGCCCACGGCCCGCCTGTCACATGTCTAGACGTCTCGGCCAACCAAGTTGCTTTTGGTGTACAGGGTCTGGGA
TGGGTGTACGAAGGAAGCAAGATCCTGGTGTATAGCCTGGAAGCAGGACGCCGCCTCTTGAAGCTGGGTAACGTTCTCCGTGACTTCACGTGTGTCAACC
TCAGCGACAGCCCTCCCAACCTCATGGTCAGTGGCAACATGGACGGGAGGGTGAGGATCCACGACCTCCGCAGTGGTAACATCGCCCTGTCGCTCTCCGC
CCATCAGCTCAGGGTCTCTGCTGTGCAGATGGATGACTGGAAGATCGTCAGTGGAGGCGAGGAAGGCCTGGTGTCCGTGTGGGATTATCGGATGAACCAG
AAGCTGTGGGAGGTGTATTCCGGGCACCCGGTGCAGCACATCTCATTCAGCAGCCACAGCCTCATCACGGCCAACGTGCCTTACCAGACGGTAATGCGAA
ACGCCGACCTGGACAGCTTCACTACTCACAGGAGACACCGGGGGCTGATCCGCGCCTATGAGTTTGCGGTGGACCAGCTGGCCTTCCAGAGCCCTCTCCC
TGTCTGCCGTTCATCCTGTGACGCCATGGCCACTCACTACTACGACCTCGCACTGGCCTTTCCCTATAACCATGTTTAGGGATGTGCCTCAGTTGGGAGC
AAGGAGAAAAATGGGAAGAACCAGTTTTATCCATCTTAAAACGCCAGGCACCTCTTCACAGGTGGTAAACATTTAGGGGAAGAAAGCAGCCCAGGGTGCC
ATGCCTGACAGCACGCATCTCCCTGACCCCTGCACTTCCCCCAGCGCCTGGGGCAAGCTGGCGTGTGCCAGGGCTCGAGTCCCACGTGCTGCCAACTCAA
ACATAGCCTCCTTCCCCACCCAGCTGGCCACCCTGGCCTCAGCTCCCTCAGGACGCCTCAGGGATCTCGCTGCGCGGTCCTATACGGTCCCTGCTTAGCC
AGCTTCTGTGTGTCCGCCCTCCCAGCTCCAGCCCTGCAGGCCGTCTTTTCCGTCTCCAGCCCCTTACCTCTTTTCCTCCGAGGGCCTTTGGATGTGCTTG
TTCCTGGCCTCCAAGGCAATAACCTCCATGTCCTTTTCCCTGTATTTACTTGGGGTGCTCTTCTGTGACACTTGCCACCACCACCTTCCAGTAGCAAGCT
GGTAGAGCTGTACTTTTTCTTCACAGCTTTTACCATGGTTTATAATTATATACTTATACAGTTTAGTCATCAATGACTATTTTGCTTACTATTGTGTCTA
TGATATGTGATACAAAGCAAATATTTTCTAAATATTCATTGACTAAACAGCCATCAAGATGAAACAGGACAGGAGAGAACTGTTCAGTGTGATACAGAGC
TGCCATTTCCAAAGTATTCTGGAAGCAAGCCATTGCCTTACTCTTGATTTTCAACAGTTCTAAACAGCAACAGCATCCACCTAATATACCTTCAGAATAT
CAAAGCGAAAACTGATAGAACTACAAAGAGAAATAAAATCCATTCTCACGGCAGGCAAATCAGTGAGAAAAATGTAAAGATCTGGAGGATTTACTTAGTA
TCGCTAACAAGCTGGATCCAGTAGATGCGTATGGAATTTCAAATACACATAGACGATGCACAAAAAACCACCCTGTACGAAGGCCACAAAGAACAGCTCA
GATGCACAAAGATTCACACCATACAGGCCAAATTATAGAATCCCCCAACCCCAAGACACTGCACCCTATGGGCTATACCCTCAGGCAGGGCCCTGCTTTT
CTAGGAATTGACTCCAATGCAGAATAGTGAGAAATCAACAAAAAGCAAAATAAGACAATTCCAGACACTCCTGAATTTACTTGGATTTAAAGAGGTTAAT
AAAAACAAACTATAAACTCTCTAGACTGGGCTGACAGTAAAAGCACAGAATACAAAACCCAGGGGATGGGGCTGGGAGGGGCTGTGAGGGGCTGGCACCT
CAGCTCAGAAAAGGTAGCAGCAATCCCAAAGCAGCAGAAGGCAGGGGTTAAATCAGGATAAAAACAGACATCAGTGGAATCGAAAACCAATAGTTAGTTA
ATATAAAGCCAGAAGGTTTTGTTTTTTTTCAGGTGGGGTCTCGCTCTGTCACCTAGACTAGACTGGAGTGCAATGGCCTGATCTCGGCTCACTGCAACCT
CTGCCTCCCGAGTTCAAGCAATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCGTCCGCTACCACAACTGGCTAATTTTTACATTTTTAGTA
GAGACGGGGGATATTGCTATGTTGGCCAGGCTGGTCTCAAACTTCTGACCTCAAATGATTGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTA
AGTCACTGTACCCAGCCCAAAAGCAGTTTAAAAAAAAAAAAGACAAAATATACAACTCTTGATAAAACATAAAGGTACAGTGGTCTATGAGGAAGAGAAA
AGGTACCTGAGGATGCAGAAGTACCTACCACATGGGAACCGTTTGTCCACACTCATTCCAGAGAAAACCGAGTCCTCTCAGTTGCACACGTGTACGTATC
AGTGGGAAGTGCTTGCCATTACTCCAAAGCCTAGAACCTTCACGTCATGAAGGTTCTGGAAGGTTTTTCAGATTGCTTAAGATACGCAGCCATTCCATAT
TCATCTCCAACTACACAGGGGAACGGAGCAGATAGAGCTGCGACTGGGAAGCGTCACCTTCCCGTCCAGAGCGCTTTCTTTCAGACCCTGCCTACCTGCA
GGCAGATGGACCGGAGGGTTTTCTGCTTCCTTTCAACCAGATAACTTCCTAAGTGGAGATGGCCTGTAGGTAGCAAATGCAGGATTTTGTTTACTTTCAT
CATGTCATGTGGTGGTCAGACTGCTCGCTGGTGGCCTCGCTTTAGAAGGTTTTCATCAAGCCCCGCCCTTTCTCTCTCATAGTCTTAATGCGTCTGGACC
ACTGGGGAAAATATTTTTCTTTTCAAAAAGCAGCCCCTTCAGTCTGCGTTCCCAGTTCATTTTGCAGGCGCATTCGCCCTTCCTAGTTTGCAGCAATTAA
AGATAACGGAGTGTAAAGTCATTAGACCCAGAATTAGAGCAGCCAGCAGCTGGCCCAGTGTAAAAATGTCCCAAGTGTAGCCCCTCCCACCGCAGAGCCT
GTTTCTAACGTACCTGCCATTCGTGGAAGTCGCCTGGGTTTTGTAAAGGAGAATTTTTTGGACTCAAGCTGTCATCTTCAAGCACGCCAACTTCAGGCAG
TGTTTTCTACTGCAAACCTCCACATCCTGGAATCAATCACACTGACAGGGAAAGGATAAATGCTCCTTTATAAGCCAACATGTATCCAAATTAAGCCCCC
TTACCTTTATCCAGACCCTTTCACAAACAAGTTCTGAGTTTTTAGCCAATTGATCCTCTACTTTCCTTCTTTAGTCCCTCTCAGGGACGCCTCCATCCCC
GGCTCCCCTAAAGGTAGAAGATACTCGAGCATGCTGCCAGGGGAGCTGGATGGTGAAACACCTGAAGATCTCATATCGCCTCTCAAAACACTGTCGGACT
TGCTTTCTGCTTTATGATGTCCGTAGTTGTTCTAATATTAAATGCTTTGAATTTC
  • refseq_FBXW8.F1 (forward primer)
  • refseq_FBXW8.R1 (reverse primer)
  • Alternative donor

Frame 0

AADTSLGGTVSWHPVEPRRTWSAGSGEYGRLQP
G*VPS
ALAGGAGAGPGAEEAATARGCREAGSAAG
GGLRARRTGLGGPGAGPASPGGRGEAPGGAGDS
GRGAGRSEPLTFSSGPRGRRGRGAAGGPAHPRP

E*NE*CAFL*YPTALRIGNQYISVSGQERTRKMC
TGEQDVEGDCRG*GAVVQAVPAGRAPSG*QHL*
LFLLEAHLPRVPSQGTHVTNQLEESQRCRERAG
ACS*HSFV*CAFSRWCGHCGIYIRGCESVGHPHL
GLRSPLPGIRGRGG*AWNAAKCLLCEDKQLVGS
SSL*GWVS*YLGFKDRKVPCSSF*ARCKNTGTS
PQPGRCNRGHSFCF*CRDVIPQ*GGVLADSCGI*
SSETGSVP*NSSRNQKVPCGSSRCWRSDVPAQS
RRLRQNPPLRPRPACHMSRRLGQPSCFWCTGSG
MGVRRKQDPGV*PGSRTPPLEAG*RSP*LHVCQP
QRQPSQPHGQWQHGREGEDPRPPQW*HRPVALR
PSAQGLCCADG*LEDRQWRRGRPGVRVGLSDEP
EAVGGVFRAPGAAHLIQQPQPHHGQRALPDGNAK
RRPGQLHYSQETPGADPRL*VCGGPAGLPEPSP
CLPFIL*RHGHSLLRPRTGLSL*PCLGMCLSWE
QGEKWEEPVLSILKRQAPLHRW*TFRGRKQPRVP
CLTARISLTPALPPAPGASWRVPGLESHVLPTQ
T*PPSPPSWPPWPQLPQDASGISLRGPIRSLLS
QLLCVRPPSSSPAGRLFRLQPLTSFPPRAFGCAC
SWPPRQ*PPCPFPCIYLGCSSVTLATTTFQ*QA
GRAVLFLHSFYHGL*LYTYTV*SSMTILLTIVS
MICDTKQIFSKYSLTKQPSR*NRTGENCSV*YRA
AISKVFWKQAIALLLIFNSSKQQQHPPNIPSEY
QSEN**NYKEK*NPFSRQANQ*EKCKDLEDLLS
IANKLDPVDAYGISNTHRRCTKNHPVRRPQRTAQ
MHKDSHHTGQIIESPNPKTLHPMGYTLRQGPAF
LGIDSNAE**EINKKQNKTIPDTPEFTWI*RG*
*KQTINSLDWADSKSTEYKTQGMGLGGAVRGWHL
SSEKVAAIPKQQKAGVKSG*KQTSVESKTNS*L
I*SQKVLFFFRWGLALSPRLDWSAMA*SRLTAT
SASRVQAILLPQPPE*LGLQASATTTG*FLHF**
RRGILLCWPGWSQTSDLK*LPASASQSAGITGV
SHCTQPKSSLKKKKTKYTTLDKT*RYSGL*GRE
KVPEDAEVPTTWEPFVHTHSRENRVLSVAHVYVS
VGSACHYSKA*NLHVMKVLEGFSDCLRYAAIPY
SSPTTQGNGADRAATGKRHLPVQSAFFQTLPTC
RQMDRRVFCFLSTR*LPKWRWPVGSKCRILFTFI
MSCGGQTARWWPRFRRFSSSPALSLS*S*CVWT
TGENIFLFKKQPLQSAFPVHFAGAFALPSLQQL
KITECKVIRPRIRAASSWPSVKMSQV*PLPPQSL
FLTYLPFVEVAWVL*RRIFWTQAVIFKHANFRQ
CFLLQTSTSWNQSH*QGKDKCSFISQHVSKLSP
LTFIQTLSQTSSEFLAN*SSTFLL*SLSGTPPSP
APLKVEDTRACCQGSWMVKHLKISYRLSKHCRT
CFLLYDVRSCSNIKCFEF

Frame 1

RRTLPWAGLSRGTRWNRGERGAPGAANMDDYSL
DEFRR
RWQEELAQAQAPKKRRRPEAAERRARRP
EVGSGRGEQASGDPALAQRLLEGAGRPPAARATR
AEGQDVASRSRSPLAREGAGGGEQLVDQLIRDL

NEMNDVPFFDIQLPYELAINIFQYLDRKELGRC
AQVSKTWKVIAEDEVLWYRLCQQEGHLPDSSISD
YSCWKLIFQECRAKEHMLRTNWKNRKGAVSELE
HVPDTVLCDVHSHDGVVIAGYTSGDVRVWDTRT
WDYVAPFLESEDEEDEPGMQPNVSFVRINSSLAV
AAYEDGFLNIWDLRTGKYPVHRFEHDARIQALA
LSQDDATVATASAFDVVMLSPNEEGYWQIAAEF
EVPKLVQYLEIVPETRRYPVAVAAAGDLMYLLKA
EDSARTLLYAHGPPVTCLDVSANQVAFGVQGLG
WVYEGSKILVYSLEAGRRLLKLGNVLRDFTCVN
LSDSPPNLMVSGNMDGRVRIHDLRSGNIALSLSA
HQLRVSAVQMDDWKIVSGGEEGLVSVWDYRMNQ
KLWEVYSGHPVQHISFSSHSLITANVPYQTVMR
NADLDSFTTHRRHRGLIRAYEFAVDQLAFQSPLP
VCRSSCDAMATHYYDLALAFPYNHV*GCASVGS
KEKNGKNQFYPS*NARHLFTGGKHLGEESSPGC
HA*QHASP*PLHFPQRLGQAGVCQGSSPTCCQLK
HSLLPHPAGHPGLSSLRTPQGSRCAVLYGPCLA
SFCVSALPAPALQAVFSVSSPLPLFLRGPLDVL
VPGLQGNNLHVLFPVFTWGALL*HLPPPPSSSKL
VELYFFFTAFTMVYNYILIQFSHQ*LFCLLLCL
*YVIQSKYFLNIH*LNSHQDETGQERTVQCDTE
LPFPKYSGSKPLPYS*FSTVLNSNSIHLIYLQNI
KAKTDRTTKRNKIHSHGRQISEKNVKIWRIYLV
SLTSWIQ*MRMEFQIHIDDAQKTTLYEGHKEQL
RCTKIHTIQAKL*NPPTPRHCTLWAIPSGRALLF
*ELTPMQNSEKSTKSKIRQFQTLLNLLGFKEVN
KNKL*TL*TGLTVKAQNTKPRGWGWEGL*GAGT
SAQKR*QQSQSSRRQGLNQDKNRHQWNRKPIVS*
YKARRFCFFSGGVSLCHLD*TGVQWPDLGSLQP
LPPEFKQFSCLSLLSSWDYRRPLPQLANFYIFS
RDGGYCYVGQAGLKLLTSNDCLPQPPKVLGLQV*
VTVPSPKAV*KKKRQNIQLLIKHKGTVVYEEEK
RYLRMQKYLPHGNRLSTLIPEKTESSQLHTCTY
QWEVLAITPKPRTFTS*RFWKVFQIA*DTQPFHI
HLQLHRGTEQIELRLGSVTFPSRALSFRPCLPA
GRWTGGFSASFQPDNFLSGDGL*VANAGFCLLS
SCHVVVRLLAGGLALEGFHQAPPFLSHSLNASGP
LGKIFFFSKSSPFSLRSQFILQAHSPFLVCSN*
R*RSVKSLDPELEQPAAGPV*KCPKCSPSHRRA
CF*RTCHSWKSPGFCKGEFFGLKLSSSSTPTSGS
VFYCKPPHPGINHTDRERINAPL*ANMYPN*AP
LPLSRPFHKQVLSF*PIDPLLSFFSPSQGRLHP
RLP*R*KILEHAARGAGW*NT*RSHIASQNTVGL
AFCFMMSVVVLILNALNF

Frame 2

GGHFPGRDCLVAPGGTEENVERRERRIWTTTAW
MSSV
GAGRRSWRRPRRRRSGDGPRLPRGGLGGR
RWAPGAANRPRGTRRWPSVSWRARGGPRRRGRL
GPRGRT*RAAHVLLWPARAPGAGSSWWTSSSAT*

MK*MMCLSLISNCLTNWQSIYFSIWTGKN*EDV
HR*ARRGR*LQRMRCCGTGCASRKGTFRIAASL
TILAGSSSSKSAEPRNTCYEPTGRIAKVP*ASWS
MFLTQFCVMCILTMVWSLRDIHQGM*ECGTPAP
GTT*PPSWNQRTRRMSLECSQMSPL*G*TARWQ
*QLMRMGFLIFGI*GPESTLFIVLSTMQEYRH*P
SARTMQPWPQLLLLMS*CYPPMRRGTGR*LRNL
KFRNWFSTLK*FQKPEGTLWQ*PLLEI*CTCSK
PKTPPEPSFTPTARLSHV*TSRPTKLLLVYRVWD
GCTKEARSWCIAWKQDAAS*SWVTFSVTSRVST
SATALPTSWSVATWTGG*GSTTSAVVTSPCRSP
PISSGSLLCRWMTGRSSVEARKAWCPCGIIG*TR
SCGRCIPGTRCSTSHSAATASSRPTCLTRR*CE
TPTWTASLLTGDTGG*SAPMSLRWTSWPSRALS
LSAVHPVTPWPLTTTTSHWPFPITMFRDVPQLGA
RRKMGRTSFIHLKTPGTSSQVVNI*GKKAAQGA
MPDSTHLPDPCTSPSAWGKLACARARVPRAANS
NIASFPTQLATLASAPSGRLRDLAARSYTVPA*P
ASVCPPSQLQPCRPSFPSPAPYLFSSEGLWMCL
FLASKAITSMSFSLYLLGVLFCDTCHHHLPVAS
W*SCTFSSQLLPWFIIIYLYSLVINDYFAYYCVY
DM*YKANIF*IFID*TAIKMKQDRRELFSVIQS
CHFQSILEASHCLTLDFQQF*TATAST*YTFRI
SKRKLIELQREIKSILTAGKSVRKM*RSGGFT*Y
R*QAGSSRCVWNFKYT*TMHKKPPCTKATKNSS
DAQRFTPYRPNYRIPQPQDTAPYGLYPQAGPCF
SRN*LQCRIVRNQQKAK*DNSRHS*IYLDLKRLI
KTNYKLSRLG*Q*KHRIQNPGDGAGRGCEGLAP
QLRKGSSNPKAAEGRG*IRIKTDISGIENQ*LV
NIKPEGFVFFQVGSRSVT*TRLECNGLISAHCNL
CLPSSSNSPASAS*VAGITGVRYHNWLIFTFLV
ETGDIAMLARLVSNF*PQMIACLSLPKCWDYRC
KSLYPAQKQFKKKKDKIYNS**NIKVQWSMRKRK
GT*GCRSTYHMGTVCPHSFQRKPSPLSCTRVRI
SGKCLPLLQSLEPSRHEGSGRFFRLLKIRSHSI
FISNYTGERSR*SCDWEASPSRPERFLSDPAYLQ
ADGPEGFLLPFNQITS*VEMACR*QMQDFVYFH
HVMWWSDCSLVASL*KVFIKPRPFSLIVLMRLD
HWGKYFSFQKAAPSVCVPSSFCRRIRPS*FAAIK
DNGV*SH*TQN*SSQQLAQCKNVPSVAPPTAEP
VSNVPAIRGSRLGFVKENFLDSSCHLQARQLQA
VFSTANLHILESITLTGKG*MLLYKPTCIQIKPP
YLYPDPFTNKF*VFSQLILYFPSLVPLRDASIP
GSPKGRRYSSMLPGELDGETPEDLISPLKTLSD
LLSAL*CP*LF*Y*ML*I

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