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Sequences

Sequence NM_198830

AGCCGATGGGGGCGGGGAAAAGTCCGGCTGGGCCGGGACAAAAGCCGGATCCCGGGAAGCTACCGGCTGCTGGGGTGCTCCGGATTTTGCGGGGTTCGTC GGGCCTGTGGAAGAAGCGCCGCGCACGGACTTCGGCAGAGGTAGAGCAGGTCTCTCTGCAGCCATGTCGGCCAAGGCAATTTCAGAGCAGACGGGCAAAG AACTCCTTTACAAGTTCATCTGTACCACCTCAGCCATCCAGAATCGGTTCAAGTATGCTCGGGTCACTCCTGACACAGACTGGGCCCGCTTGCTGCAGGA CCACCCCTGGCTGCTCAGCCAGAACTTGGTAGTCAAGCCAGACCAGCTGATCAAACGTCGTGGAAAACTTGGTCTCGTTGGGGTCAACCTCACTCTGGAT GGGGTCAAGTCCTGGCTGAAGCCACGGCTGGGACAGGAAGCCACAGTTGGCAAGGCCACAGGCTTCCTCAAGAACTTTCTGATCGAGCCCTTCGTCCCCC ACAGTCAGGCTGAGGAGTTCTATGTCTGCATCTATGCCACCCGAGAAGGGGACTACGTCCTGTTCCACCACGAGGGGGGTGTGGACGTGGGTGATGTGGA CGCCAAGGCCCAGAAGCTGCTTGTTGGCGTGGATGAGAAACTGAATCCTGAGGACATCAAAAAACACCTGTTGGTCCACGCCCCTGAAGACAAGAAAGAA ATTCTGGCCAGTTTTATCTCCGGCCTCTTCAATTTCTACGAGGACTTGTACTTCACCTACCTCGAGATCAATCCCCTTGTAGTGACCAAAGATGGAGTCT ATGTCCTTGACTTGGCGGCCAAGGTGGACGCCACTGCCGACTACATCTGCAAAGTGAAGTGGGGTGACATCGAGTTCCCTCCCCCCTTCGGGCGGGAGGC ATATCCAGAGGAAGCCTACATTGCAGACCTCGATGCCAAAAGTGGGGCAAGCCTGAAGCTGACCTTGCTGAACCCCAAAGGGAGGATCTGGACCATGGTG GCCGGGGGTGGCGCCTCTGTCGTGTACAGCGATACCATCTGTGATCTAGGGGGTGTCAACGAGCTGGCAAACTATGGGGAGTACTCAGGCGCCCCCAGCG AGCAGCAGACCTATGACTATGCCAAGACTATCCTCTCCCTCATGACCCGAGAGAAGCACCCAGATGGCAAGATCCTCATCATTGGAGGCAGCATCGCAAA CTTCACCAACGTGGCTGCCACGTTCAAGGGCATCGTGAGAGCAATTCGAGATTACCAGGGCCCCCTGAAGGAGCACGAAGTCACAATCTTTGTCCGAAGA GGTGGCCCCAACTATCAGGAGGGCTTACGGGTGATGGGAGAAGTCGGGAAGACCACTGGGATCCCCATCCATGTCTTTGGCACAGAGACTCACATGACGG CCATTGTGGGCATGGCCCTGGGCCACCGGCCCATCCCCAACCAGCCACCCACAGCGGCCCACACTGCAAACTTCCTCCTCAACGCCAGCGGGAGCACATC GACGCCAGCCCCCAGCAGGACAGCATCTTTTTCTGAGTCCAGGGCCGATGAGGTGGCGCCTGCAAAGAAGGCCAAGCCTGCCATGCCACAAGGAAAGAGC ACCACCCTCTTCAGCCGCCACACCAAGGCCATTGTGTGGGGCATGCAGACCCGGGCCGTGCAAGGCATGCTGGACTTTGACTATGTCTGCTCCCGAGACG AGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGACCACAAGCAGAAGTTTTACTGGGGGCACAAAGAGATCCTGATCCCTGTCTTCAAGAACAT GGCTGATGCCATGAGGAAGCATCCGGAGGTAGATGTGCTCATCAACTTTGCCTCTCTCCGCTCTGCCTATGACAGCACCATGGAGACCATGAACTATGCC CAGATCCGGACCATCGCCATCATAGCTGAAGGCATCCCTGAGGCCCTCACGAGAAAGCTGATCAAGAAGGCGGACCAGAAGGGAGTGACCATCATCGGAC CTGCCACTGTTGGAGGCATCAAGCCTGGGTGCTTTAAGATTGGCAACACAGGTGGGATGCTGGACAACATCCTGGCCTCCAAACTGTACCGCCCAGGCAG CGTGGCCTATGTCTCACGTTCCGGAGGCATGTCCAACGAGCTCAACAATATCATCTCTCGGACCACGGATGGCGTCTATGAGGGCGTGGCCATTGGTGGG GACAGGTACCCGGGCTCCACATTCATGGATCATGTGTTACGCTATCAGGACACTCCAGGAGTCAAAATGATTGTGGTTCTTGGAGAGATTGGGGGCACTG AGGAATATAAGATTTGCCGGGGCATCAAGGAGGGCCGCCTCACTAAGCCCATCGTCTGCTGGTGCATCGGGACGTGTGCCACCATGTTCTCCTCTGAGGT CCAGTTTGGCCATGCTGGAGCTTGTGCCAACCAGGCTTCTGAAACTGCAGTAGCCAAGAACCAGGCTTTGAAGGAAGCAGGAGTGTTTGTGCCCCGGAGC TTTGATGAGCTTGGAGAGATCATCCAGTCTGTATACGAAGATCTCGTGGCCAATGGAGTCATTGTACCTGCCCAGGAGGTGCCGCCCCCAACCGTGCCCA TGGACTACTCCTGGGCCAGGGAGCTTGGTTTGATCCGCAAACCTGCCTCGTTCATGACCAGCATCTGCGATGAGCGAGGACAGGAGCTCATCTACGCGGG CATGCCCATCACTGAGGTCTTCAAGGAAGAGATGGGCATTGGCGGGGTCCTCGGCCTCCTCTGGTTCCAGAAAAGGTTGCCTAAGTACTCTTGCCAGTTC ATTGAGATGTGTCTGATGGTGACAGCTGATCACGGGCCAGCCGTCTCTGGAGCCCACAACACCATCATTTGTGCGCGAGCTGGGAAAGACCTGGTCTCCA GCCTCACCTCGGGGCTGCTCACCATCGGGGATCGGTTTGGGGGTGCCTTGGATGCAGCAGCCAAGATGTTCAGTAAAGCCTTTGACAGTGGCATTATCCC CATGGAGTTTGTGAACAAGATGAAGAAGGAAGGGAAGCTGATCATGGGCATTGGTCACCGAGTGAAGTCGATAAACAACCCAGACATGCGAGTGCAGATC CTCAAAGATTACGTCAGGCAGCACTTCCCTGCCACTCCTCTGCTCGATTATGCACTGGAAGTAGAGAAGATTACCACCTCGAAGAAGCCAAATCTTATCC TGAATGTAGATGGTCTCATCGGAGTCGCATTTGTAGACATGCTTAGAAACTGTGGGTCCTTTACTCGGGAGGAAGCTGATGAATATATTGACATTGGAGC CCTCAATGGCATCTTTGTGCTGGGAAGGAGTATGGGGTTCATTGGACACTATCTTGATCAGAAGAGGCTGAAGCAGGGGCTGTATCGTCATCCGTGGGAT GATATTTCATATGTTCTTCCGGAACACATGAGCATGTAACAGAGCCAGGAACCCTACTGCAGTAAACTGAAGACAAGATCTCTTCCCCCAAGAAAAAGTG TACAGACAGCTGGCAGTGGAGCCTGCTTTATTTAGCAGGGGCCTGGAATGTAAACAGCCACTGGGGTACAGGCACCGAAGACCAACATCCACAGGCTAAC ACCCCTTCAGTCCACACAAAGAAGCTTCATATTTTTTTTATAAGCATAGAAATAAAAACCAAGCCAATATTTGTGACTTTGCTCTGCTACCTGCTGTATT TATTATATGGAAGCATCTAAGTACTGTCAGGATGGGGTCTTCCTCATTGTAGGGCGTTAGGATGTTGCTTTCTTTTTCCATTAGTTAAACATTTTTTTCT CCTTTGGAGGAAGGGAATGAAACATTTATGGCCTCAAGATACTATACATTTAAAGCACCCCAATGTCTCTCTTTTTTTTTTTTTACTTCCCTTTCTTCTT CCTTATATAACATGAAGAACATTGTATTAATCTGATTTTTAAAGATCTTTTTGTATGTTACGTGTTAAGGGCTTGTTTGGTATCCCACTGAAATGTTCTG TGTTGCAGACCAGAGTCTGTTTATGTCAGGGGGATGGGGCCATTGCATCCTTAGCCATTGTCACAAAATATGTGGAGTAGTAACTTAATATGTAAAGTTG TAACATACATACATTTAAAATGGAAATGCAGAAAGCTGTGAAATGTCTTGTGTCTTATGTTCTCTGTATTTATGCAGCTGATTTGTCTGTCTGTAACTGA AGTGTGGGTCCAAGGACTCCTAACTACTTTGCATCTGTAATCCACAAAGATTCTGGGCAGCTGCCACCTCAGTCTCTTCTCTGTATTATCATAGTCTGGT TTAAATAAACTATATAGTAACAA

refseq_ACLY.F1 (forward primer)
refseq_ACLY.R1 (reverse primer)
Single exon skipping

Frame 0

SRWGRGKVRLGRDKSRIPGSYRLLGCSGFCGVR RACGRSAAHGLRQR*SRSLCSHVGQGNFRADGQR TPLQVHLYHLSHPESVQVCSGHS*HRLGPLAAG PPLAAQPELGSQARPADQTSWKTWSRWGQPHSG WGQVLAEATAGTGSHSWQGHRLPQELSDRALRPP QSG*GVLCLHLCHPRRGLRPVPPRGGCGRG*CG RQGPEAACWRG*ETES*GHQKTPVGPRP*RQER NSGQFYLRPLQFLRGLVLHLPRDQSPCSDQRWSL CP*LGGQGGRHCRLHLQSEVG*HRVPSPLRAGG ISRGSLHCRPRCQKWGKPEADLAEPQREDLDHG GRGWRLCRVQRYHL*SRGCQRAGKLWGVLRRPQR AADL*LCQDYPLPHDPREAPRWQDPHHWRQHRK LHQRGCHVQGHRESNSRLPGPPEGARSHNLCPK RWPQLSGGLTGDGRSREDHWDPHPCLWHRDSHDG HCGHGPGPPAHPQPATHSGPHCKLPPQRQREHI DASPQQDSIFF*VQGR*GGACKEGQACHATRKE HHPLQPPHQGHCVGHADPGRARHAGL*LCLLPRR ALSGCHGLPFHWGPQAEVLLGAQRDPDPCLQEH G*CHEEASGGRCAHQLCLSPLCL*QHHGDHELC PDPDHRHHS*RHP*GPHEKADQEGGPEGSDHHRT CHCWRHQAWVL*DWQHRWDAGQHPGLQTVPPRQ RGLCLTFRRHVQRAQQYHLSDHGWRL*GRGHWW GQVPGLHIHGSCVTLSGHSRSQNDCGSWRDWGH* GI*DLPGHQGGPPH*AHRLLVHRDVCHHVLL*G PVWPCWSLCQPGF*NCSSQEPGFEGSRSVCAPE L**AWRDHPVCIRRSRGQWSHCTCPGGAAPNRAH GLLLGQGAWFDPQTCLVHDQHLR*ARTGAHLRG HAHH*GLQGRDGHWRGPRPPLVPEKVA*VLLPV H*DVSDGDS*SRASRLWSPQHHHLCASWERPGLQ PHLGAAHHRGSVWGCLGCSSQDVQ*SL*QWHYP HGVCEQDEEGREADHGHWSPSEVDKQPRHASAD PQRLRQAALPCHSSARLCTGSREDYHLEEAKSYP ECRWSHRSRICRHA*KLWVLYSGGS**IY*HWS PQWHLCAGKEYGVHWTLS*SEEAEAGAVSSSVG *YFICSSGTHEHVTEPGTLLQ*TEDKISSPKKKC TDSWQWSLLYLAGAWNVNSHWGTGTEDQHPQAN TPSVHTKKLHIFFISIEIKTKPIFVTLLCYLLY LLYGSI*VLSGWGLPHCRALGCCFLFPLVKHFFL LWRKGMKHLWPQDTIHLKHPNVSLFFFLLPFLL PYIT*RTLY*SDF*RSFCMLRVKGLFGIPLKCS VLQTRVCLCQGDGAIASLAIVTKYVE**LNM*SC NIHTFKMEMQKAVKCLVSYVLCIYAADLSVCN* SVGPRTPNYFASVIHKDSGQLPPQSLLCIIIVW FK*TI**Q

Frame 1

ADGGGEKSGWAGTKAGSREATGCWGAPDFAGFV GPVEEAPRTDFGRGRAGLSAAMSAKAISEQTGK ELLYKFICTTSAIQNRFKYARVTPDTDWARLLQD HPWLLSQNLVVKPDQLIKRRGKLGLVGVNLTLD GVKSWLKPRLGQEATVGKATGFLKNFLIEPFVP HSQAEEFYVCIYATREGDYVLFHHEGGVDVGDVD AKAQKLLVGVDEKLNPEDIKKHLLVHAPEDKKE ILASFISGLFNFYEDLYFTYLEINPLVVTKDGV YVLDLAAKVDATADYICKVKWGDIEFPPPFGREA YPEEAYIADLDAKSGASLKLTLLNPKGRIWTMV AGGGASVVYSDTICDLGGVNELANYGEYSGAPS EQQTYDYAKTILSLMTREKHPDGKILIIGGSIAN FTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRR GGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMT AIVGMALGHRPIPNQPPTAAHTANFLLNASGSTS TPAPSRTASFSESRADEVAPAKKAKPAMPQGKS TTLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRD EPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNM ADAMRKHPEVDVLINFASLRSAYDSTMETMNYA QIRTIAIIAEGIPEALTRKLIKKADQKGVTIIG PATVGGIKPGCFKIGNTGGMLDNILASKLYRPGS VAYVSRSGGMSNELNNIISRTTDGVYEGVAIGG DRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGT EEYKICRGIKEGRLTKPIVCWCIGTCATMFSSEV QFGHAGACANQASETAVAKNQALKEAGVFVPRS FDELGEIIQSVYEDLVANGVIVPAQEVPPPTVP MDYSWARELGLIRKPASFMTSICDERGQELIYAG MPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQF IEMCLMVTADHGPAVSGAHNTIICARAGKDLVS SLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIP MEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQI LKDYVRQHFPATPLLDYALEVEKITTSKKPNLI LNVDGLIGVAFVDMLRNCGSFTREEADEYIDIGA LNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWD DISYVLPEHMSM*QSQEPYCSKLKTRSLPPRKS VQTAGSGACFI*QGPGM*TATGVQAPKTNIHRLT PLQSTQRSFIFFL*A*K*KPSQYL*LCSATCCI YYMEASKYCQDGVFLIVGR*DVAFFFH*LNIFF SFGGRE*NIYGLKILYI*STPMSLFFFFYFPFFF LI*HEEHCINLIFKDLFVCYVLRACLVSH*NVL CCRPESVYVRGMGPLHP*PLSQNMWSSNLICKV VTYIHLKWKCRKL*NVLCLMFSVFMQLICLSVTE VWVQGLLTTLHL*STKILGSCHLSLFSVLS*SG LNKLYSN

Frame 2

PMGAGKSPAGPGQKPDPGKLPAAGVLRILRGSS GLWKKRRARTSAEVEQVSLQPCRPRQFQSRRAK NSFTSSSVPPQPSRIGSSMLGSLLTQTGPACCR TTPGCSARTW*SSQTS*SNVVENLVSLGSTSLWM GSSPG*SHGWDRKPQLARPQASSRTF*SSPSSP TVRLRSSMSASMPPEKGTTSCSTTRGVWTWVMW TPRPRSCLLAWMRN*ILRTSKNTCWSTPLKTRKK FWPVLSPASSISTRTCTSPTSRSIPL**PKMES MSLTWRPRWTPLPTTSAK*SGVTSSSLPPSGGR HIQRKPTLQTSMPKVGQA*S*PC*TPKGGSGPWW PGVAPLSCTAIPSVI*GVSTSWQTMGSTQAPPA SSRPMTMPRLSSPS*PERSTQMARSSSLEAASQ TSPTWLPRSRAS*EQFEITRAP*RSTKSQSLSEE VAPTIRRAYG*WEKSGRPLGSPSMSLAQRLT*R PLWAWPWATGPSPTSHPQRPTLQTSSSTPAGAH RRQPPAGQHLFLSPGPMRWRLQRRPSLPCHKERA PPSSAATPRPLCGACRPGPCKACWTLTMSAPET SPQWLPWSTLSLGTTSRSFTGGTKRS*SLSSRT WLMP*GSIRR*MCSSTLPLSALPMTAPWRP*TMP RSGPSPS*LKASLRPSRES*SRRRTRRE*PSSD LPLLEASSLGALRLATQVGCWTTSWPPNCTAQA AWPMSHVPEACPTSSTISSLGPRMASMRAWPLVG TGTRAPHSWIMCYAIRTLQESK*LWFLERLGAL RNIRFAGASRRAASLSPSSAGASGRVPPCSPLR SSLAMLELVPTRLLKLQ*PRTRL*RKQECLCPGA LMSLERSSSLYTKISWPMESLYLPRRCRPQPCP WTTPGPGSLV*SANLPRS*PASAMSEDRSSSTR ACPSLRSSRKRWALAGSSASSGSRKGCLSTLASS LRCV*W*QLITGQPSLEPTTPSFVRELGKTWSP ASPRGCSPSGIGLGVPWMQQPRCSVKPLTVALS PWSL*TR*RRKGS*SWALVTE*SR*TTQTCECRS SKITSGSTSLPLLCSIMHWK*RRLPPRRSQILS *M*MVSSESHL*TCLETVGPLLGRKLMNILTLE PSMASLCWEGVWGSLDTILIRRG*SRGCIVIRGM IFHMFFRNT*ACNRARNPTAVN*RQDLFPQEKV YRQLAVEPALFSRGLECKQPLGYRHRRPTSTG* HPFSPHKEASYFFYKHRNKNQANICDFALLPAVF IIWKHLSTVRMGSSSL*GVRMLLSFSIS*TFFS PLEEGNETFMASRYYTFKAPQCLSFFFFTSLSS SLYNMKNIVLI*FLKIFLYVTC*GLVWYPTEMFC VADQSLFMSGGWGHCILSHCHKICGVVT*YVKL *HTYI*NGNAESCEMSCVLCSLYLCS*FVCL*L KCGSKDS*LLCICNPQRFWAAATSVSSLYYHSLV *INYIVT

Sequence NM_001096

AGCCGATGGGGGCGGGGAAAAGTCCGGCTGGGCCGGGACAAAAGCCGGATCCCGGGAAGCTACCGGCTGCTGGGGTGCTCCGGATTTTGCGGGGTTCGTC GGGCCTGTGGAAGAAGCGCCGCGCACGGACTTCGGCAGAGGTAGAGCAGGTCTCTCTGCAGCCATGTCGGCCAAGGCAATTTCAGAGCAGACGGGCAAAG AACTCCTTTACAAGTTCATCTGTACCACCTCAGCCATCCAGAATCGGTTCAAGTATGCTCGGGTCACTCCTGACACAGACTGGGCCCGCTTGCTGCAGGA CCACCCCTGGCTGCTCAGCCAGAACTTGGTAGTCAAGCCAGACCAGCTGATCAAACGTCGTGGAAAACTTGGTCTCGTTGGGGTCAACCTCACTCTGGAT GGGGTCAAGTCCTGGCTGAAGCCACGGCTGGGACAGGAAGCCACAGTTGGCAAGGCCACAGGCTTCCTCAAGAACTTTCTGATCGAGCCCTTCGTCCCCC ACAGTCAGGCTGAGGAGTTCTATGTCTGCATCTATGCCACCCGAGAAGGGGACTACGTCCTGTTCCACCACGAGGGGGGTGTGGACGTGGGTGATGTGGA CGCCAAGGCCCAGAAGCTGCTTGTTGGCGTGGATGAGAAACTGAATCCTGAGGACATCAAAAAACACCTGTTGGTCCACGCCCCTGAAGACAAGAAAGAA ATTCTGGCCAGTTTTATCTCCGGCCTCTTCAATTTCTACGAGGACTTGTACTTCACCTACCTCGAGATCAATCCCCTTGTAGTGACCAAAGATGGAGTCT ATGTCCTTGACTTGGCGGCCAAGGTGGACGCCACTGCCGACTACATCTGCAAAGTGAAGTGGGGTGACATCGAGTTCCCTCCCCCCTTCGGGCGGGAGGC ATATCCAGAGGAAGCCTACATTGCAGACCTCGATGCCAAAAGTGGGGCAAGCCTGAAGCTGACCTTGCTGAACCCCAAAGGGAGGATCTGGACCATGGTG GCCGGGGGTGGCGCCTCTGTCGTGTACAGCGATACCATCTGTGATCTAGGGGGTGTCAACGAGCTGGCAAACTATGGGGAGTACTCAGGCGCCCCCAGCG AGCAGCAGACCTATGACTATGCCAAGACTATCCTCTCCCTCATGACCCGAGAGAAGCACCCAGATGGCAAGATCCTCATCATTGGAGGCAGCATCGCAAA CTTCACCAACGTGGCTGCCACGTTCAAGGGCATCGTGAGAGCAATTCGAGATTACCAGGGCCCCCTGAAGGAGCACGAAGTCACAATCTTTGTCCGAAGA GGTGGCCCCAACTATCAGGAGGGCTTACGGGTGATGGGAGAAGTCGGGAAGACCACTGGGATCCCCATCCATGTCTTTGGCACAGAGACTCACATGACGG CCATTGTGGGCATGGCCCTGGGCCACCGGCCCATCCCCAACCAGCCACCCACAGCGGCCCACACTGCAAACTTCCTCCTCAACGCCAGCGGGAGCACATC GACGCCAGCCCCCAGCAGGACAGCATCTTTTTCTGAGTCCAGGGCCGATGAGGTGGCGCCTGCAAAGAAGGCCAAGCCTGCCATGCCACAAGATTCAGTC CCAAGTCCAAGATCCCTGCAAGGAAAGAGCACCACCCTCTTCAGCCGCCACACCAAGGCCATTGTGTGGGGCATGCAGACCCGGGCCGTGCAAGGCATGC TGGACTTTGACTATGTCTGCTCCCGAGACGAGCCCTCAGTGGCTGCCATGGTCTACCCTTTCACTGGGGACCACAAGCAGAAGTTTTACTGGGGGCACAA AGAGATCCTGATCCCTGTCTTCAAGAACATGGCTGATGCCATGAGGAAGCATCCGGAGGTAGATGTGCTCATCAACTTTGCCTCTCTCCGCTCTGCCTAT GACAGCACCATGGAGACCATGAACTATGCCCAGATCCGGACCATCGCCATCATAGCTGAAGGCATCCCTGAGGCCCTCACGAGAAAGCTGATCAAGAAGG CGGACCAGAAGGGAGTGACCATCATCGGACCTGCCACTGTTGGAGGCATCAAGCCTGGGTGCTTTAAGATTGGCAACACAGGTGGGATGCTGGACAACAT CCTGGCCTCCAAACTGTACCGCCCAGGCAGCGTGGCCTATGTCTCACGTTCCGGAGGCATGTCCAACGAGCTCAACAATATCATCTCTCGGACCACGGAT GGCGTCTATGAGGGCGTGGCCATTGGTGGGGACAGGTACCCGGGCTCCACATTCATGGATCATGTGTTACGCTATCAGGACACTCCAGGAGTCAAAATGA TTGTGGTTCTTGGAGAGATTGGGGGCACTGAGGAATATAAGATTTGCCGGGGCATCAAGGAGGGCCGCCTCACTAAGCCCATCGTCTGCTGGTGCATCGG GACGTGTGCCACCATGTTCTCCTCTGAGGTCCAGTTTGGCCATGCTGGAGCTTGTGCCAACCAGGCTTCTGAAACTGCAGTAGCCAAGAACCAGGCTTTG AAGGAAGCAGGAGTGTTTGTGCCCCGGAGCTTTGATGAGCTTGGAGAGATCATCCAGTCTGTATACGAAGATCTCGTGGCCAATGGAGTCATTGTACCTG CCCAGGAGGTGCCGCCCCCAACCGTGCCCATGGACTACTCCTGGGCCAGGGAGCTTGGTTTGATCCGCAAACCTGCCTCGTTCATGACCAGCATCTGCGA TGAGCGAGGACAGGAGCTCATCTACGCGGGCATGCCCATCACTGAGGTCTTCAAGGAAGAGATGGGCATTGGCGGGGTCCTCGGCCTCCTCTGGTTCCAG AAAAGGTTGCCTAAGTACTCTTGCCAGTTCATTGAGATGTGTCTGATGGTGACAGCTGATCACGGGCCAGCCGTCTCTGGAGCCCACAACACCATCATTT GTGCGCGAGCTGGGAAAGACCTGGTCTCCAGCCTCACCTCGGGGCTGCTCACCATCGGGGATCGGTTTGGGGGTGCCTTGGATGCAGCAGCCAAGATGTT CAGTAAAGCCTTTGACAGTGGCATTATCCCCATGGAGTTTGTGAACAAGATGAAGAAGGAAGGGAAGCTGATCATGGGCATTGGTCACCGAGTGAAGTCG ATAAACAACCCAGACATGCGAGTGCAGATCCTCAAAGATTACGTCAGGCAGCACTTCCCTGCCACTCCTCTGCTCGATTATGCACTGGAAGTAGAGAAGA TTACCACCTCGAAGAAGCCAAATCTTATCCTGAATGTAGATGGTCTCATCGGAGTCGCATTTGTAGACATGCTTAGAAACTGTGGGTCCTTTACTCGGGA GGAAGCTGATGAATATATTGACATTGGAGCCCTCAATGGCATCTTTGTGCTGGGAAGGAGTATGGGGTTCATTGGACACTATCTTGATCAGAAGAGGCTG AAGCAGGGGCTGTATCGTCATCCGTGGGATGATATTTCATATGTTCTTCCGGAACACATGAGCATGTAACAGAGCCAGGAACCCTACTGCAGTAAACTGA AGACAAGATCTCTTCCCCCAAGAAAAAGTGTACAGACAGCTGGCAGTGGAGCCTGCTTTATTTAGCAGGGGCCTGGAATGTAAACAGCCACTGGGGTACA GGCACCGAAGACCAACATCCACAGGCTAACACCCCTTCAGTCCACACAAAGAAGCTTCATATTTTTTTTATAAGCATAGAAATAAAAACCAAGCCAATAT TTGTGACTTTGCTCTGCTACCTGCTGTATTTATTATATGGAAGCATCTAAGTACTGTCAGGATGGGGTCTTCCTCATTGTAGGGCGTTAGGATGTTGCTT TCTTTTTCCATTAGTTAAACATTTTTTTCTCCTTTGGAGGAAGGGAATGAAACATTTATGGCCTCAAGATACTATACATTTAAAGCACCCCAATGTCTCT CTTTTTTTTTTTTTACTTCCCTTTCTTCTTCCTTATATAACATGAAGAACATTGTATTAATCTGATTTTTAAAGATCTTTTTGTATGTTACGTGTTAAGG GCTTGTTTGGTATCCCACTGAAATGTTCTGTGTTGCAGACCAGAGTCTGTTTATGTCAGGGGGATGGGGCCATTGCATCCTTAGCCATTGTCACAAAATA TGTGGAGTAGTAACTTAATATGTAAAGTTGTAACATACATACATTTAAAATGGAAATGCAGAAAGCTGTGAAATGTCTTGTGTCTTATGTTCTCTGTATT TATGCAGCTGATTTGTCTGTCTGTAACTGAAGTGTGGGTCCAAGGACTCCTAACTACTTTGCATCTGTAATCCACAAAGATTCTGGGCAGCTGCCACCTC AGTCTCTTCTCTGTATTATCATAGTCTGGTTTAAATAAACTATATAGTAACAA

refseq_ACLY.F1 (forward primer)
refseq_ACLY.R1 (reverse primer)
Single exon skipping

Frame 0

SRWGRGKVRLGRDKSRIPGSYRLLGCSGFCGVR RACGRSAAHGLRQR*SRSLCSHVGQGNFRADGQR TPLQVHLYHLSHPESVQVCSGHS*HRLGPLAAG PPLAAQPELGSQARPADQTSWKTWSRWGQPHSG WGQVLAEATAGTGSHSWQGHRLPQELSDRALRPP QSG*GVLCLHLCHPRRGLRPVPPRGGCGRG*CG RQGPEAACWRG*ETES*GHQKTPVGPRP*RQER NSGQFYLRPLQFLRGLVLHLPRDQSPCSDQRWSL CP*LGGQGGRHCRLHLQSEVG*HRVPSPLRAGG ISRGSLHCRPRCQKWGKPEADLAEPQREDLDHG GRGWRLCRVQRYHL*SRGCQRAGKLWGVLRRPQR AADL*LCQDYPLPHDPREAPRWQDPHHWRQHRK LHQRGCHVQGHRESNSRLPGPPEGARSHNLCPK RWPQLSGGLTGDGRSREDHWDPHPCLWHRDSHDG HCGHGPGPPAHPQPATHSGPHCKLPPQRQREHI DASPQQDSIFF*VQGR*GGACKEGQACHATRFS PKSKIPARKEHHPLQPPHQGHCVGHADPGRARHA GL*LCLLPRRALSGCHGLPFHWGPQAEVLLGAQ RDPDPCLQEHG*CHEEASGGRCAHQLCLSPLCL *QHHGDHELCPDPDHRHHS*RHP*GPHEKADQEG GPEGSDHHRTCHCWRHQAWVL*DWQHRWDAGQH PGLQTVPPRQRGLCLTFRRHVQRAQQYHLSDHG WRL*GRGHWWGQVPGLHIHGSCVTLSGHSRSQND CGSWRDWGH*GI*DLPGHQGGPPH*AHRLLVHR DVCHHVLL*GPVWPCWSLCQPGF*NCSSQEPGF EGSRSVCAPEL**AWRDHPVCIRRSRGQWSHCTC PGGAAPNRAHGLLLGQGAWFDPQTCLVHDQHLR *ARTGAHLRGHAHH*GLQGRDGHWRGPRPPLVP EKVA*VLLPVH*DVSDGDS*SRASRLWSPQHHHL CASWERPGLQPHLGAAHHRGSVWGCLGCSSQDV Q*SL*QWHYPHGVCEQDEEGREADHGHWSPSEV DKQPRHASADPQRLRQAALPCHSSARLCTGSRED YHLEEAKSYPECRWSHRSRICRHA*KLWVLYSG GS**IY*HWSPQWHLCAGKEYGVHWTLS*SEEA EAGAVSSSVG*YFICSSGTHEHVTEPGTLLQ*TE DKISSPKKKCTDSWQWSLLYLAGAWNVNSHWGT GTEDQHPQANTPSVHTKKLHIFFISIEIKTKPI FVTLLCYLLYLLYGSI*VLSGWGLPHCRALGCCF LFPLVKHFFLLWRKGMKHLWPQDTIHLKHPNVS LFFFLLPFLLPYIT*RTLY*SDF*RSFCMLRVK GLFGIPLKCSVLQTRVCLCQGDGAIASLAIVTKY VE**LNM*SCNIHTFKMEMQKAVKCLVSYVLCI YAADLSVCN*SVGPRTPNYFASVIHKDSGQLPP QSLLCIIIVWFK*TI**Q

Frame 1

ADGGGEKSGWAGTKAGSREATGCWGAPDFAGFV GPVEEAPRTDFGRGRAGLSAAMSAKAISEQTGK ELLYKFICTTSAIQNRFKYARVTPDTDWARLLQD HPWLLSQNLVVKPDQLIKRRGKLGLVGVNLTLD GVKSWLKPRLGQEATVGKATGFLKNFLIEPFVP HSQAEEFYVCIYATREGDYVLFHHEGGVDVGDVD AKAQKLLVGVDEKLNPEDIKKHLLVHAPEDKKE ILASFISGLFNFYEDLYFTYLEINPLVVTKDGV YVLDLAAKVDATADYICKVKWGDIEFPPPFGREA YPEEAYIADLDAKSGASLKLTLLNPKGRIWTMV AGGGASVVYSDTICDLGGVNELANYGEYSGAPS EQQTYDYAKTILSLMTREKHPDGKILIIGGSIAN FTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRR GGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMT AIVGMALGHRPIPNQPPTAAHTANFLLNASGSTS TPAPSRTASFSESRADEVAPAKKAKPAMPQDSV PSPRSLQGKSTTLFSRHTKAIVWGMQTRAVQGM LDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHK EILIPVFKNMADAMRKHPEVDVLINFASLRSAY DSTMETMNYAQIRTIAIIAEGIPEALTRKLIKK ADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNI LASKLYRPGSVAYVSRSGGMSNELNNIISRTTD GVYEGVAIGGDRYPGSTFMDHVLRYQDTPGVKM IVVLGEIGGTEEYKICRGIKEGRLTKPIVCWCIG TCATMFSSEVQFGHAGACANQASETAVAKNQAL KEAGVFVPRSFDELGEIIQSVYEDLVANGVIVP AQEVPPPTVPMDYSWARELGLIRKPASFMTSICD ERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQ KRLPKYSCQFIEMCLMVTADHGPAVSGAHNTII CARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMF SKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKS INNPDMRVQILKDYVRQHFPATPLLDYALEVEK ITTSKKPNLILNVDGLIGVAFVDMLRNCGSFTRE EADEYIDIGALNGIFVLGRSMGFIGHYLDQKRL KQGLYRHPWDDISYVLPEHMSM*QSQEPYCSKL KTRSLPPRKSVQTAGSGACFI*QGPGM*TATGVQ APKTNIHRLTPLQSTQRSFIFFL*A*K*KPSQY L*LCSATCCIYYMEASKYCQDGVFLIVGR*DVA FFFH*LNIFFSFGGRE*NIYGLKILYI*STPMSL FFFFYFPFFFLI*HEEHCINLIFKDLFVCYVLR ACLVSH*NVLCCRPESVYVRGMGPLHP*PLSQN MWSSNLICKVVTYIHLKWKCRKL*NVLCLMFSVF MQLICLSVTEVWVQGLLTTLHL*STKILGSCHL SLFSVLS*SGLNKLYSN

Frame 2

PMGAGKSPAGPGQKPDPGKLPAAGVLRILRGSS GLWKKRRARTSAEVEQVSLQPCRPRQFQSRRAK NSFTSSSVPPQPSRIGSSMLGSLLTQTGPACCR TTPGCSARTW*SSQTS*SNVVENLVSLGSTSLWM GSSPG*SHGWDRKPQLARPQASSRTF*SSPSSP TVRLRSSMSASMPPEKGTTSCSTTRGVWTWVMW TPRPRSCLLAWMRN*ILRTSKNTCWSTPLKTRKK FWPVLSPASSISTRTCTSPTSRSIPL**PKMES MSLTWRPRWTPLPTTSAK*SGVTSSSLPPSGGR HIQRKPTLQTSMPKVGQA*S*PC*TPKGGSGPWW PGVAPLSCTAIPSVI*GVSTSWQTMGSTQAPPA SSRPMTMPRLSSPS*PERSTQMARSSSLEAASQ TSPTWLPRSRAS*EQFEITRAP*RSTKSQSLSEE VAPTIRRAYG*WEKSGRPLGSPSMSLAQRLT*R PLWAWPWATGPSPTSHPQRPTLQTSSSTPAGAH RRQPPAGQHLFLSPGPMRWRLQRRPSLPCHKIQS QVQDPCKERAPPSSAATPRPLCGACRPGPCKAC WTLTMSAPETSPQWLPWSTLSLGTTSRSFTGGT KRS*SLSSRTWLMP*GSIRR*MCSSTLPLSALPM TAPWRP*TMPRSGPSPS*LKASLRPSRES*SRR RTRRE*PSSDLPLLEASSLGALRLATQVGCWTT SWPPNCTAQAAWPMSHVPEACPTSSTISSLGPRM ASMRAWPLVGTGTRAPHSWIMCYAIRTLQESK* LWFLERLGALRNIRFAGASRRAASLSPSSAGAS GRVPPCSPLRSSLAMLELVPTRLLKLQ*PRTRL* RKQECLCPGALMSLERSSSLYTKISWPMESLYL PRRCRPQPCPWTTPGPGSLV*SANLPRS*PASA MSEDRSSSTRACPSLRSSRKRWALAGSSASSGSR KGCLSTLASSLRCV*W*QLITGQPSLEPTTPSF VRELGKTWSPASPRGCSPSGIGLGVPWMQQPRC SVKPLTVALSPWSL*TR*RRKGS*SWALVTE*SR *TTQTCECRSSKITSGSTSLPLLCSIMHWK*RR LPPRRSQILS*M*MVSSESHL*TCLETVGPLLG RKLMNILTLEPSMASLCWEGVWGSLDTILIRRG* SRGCIVIRGMIFHMFFRNT*ACNRARNPTAVN* RQDLFPQEKVYRQLAVEPALFSRGLECKQPLGY RHRRPTSTG*HPFSPHKEASYFFYKHRNKNQANI CDFALLPAVFIIWKHLSTVRMGSSSL*GVRMLL SFSIS*TFFSPLEEGNETFMASRYYTFKAPQCL SFFFFTSLSSSLYNMKNIVLI*FLKIFLYVTC*G LVWYPTEMFCVADQSLFMSGGWGHCILSHCHKI CGVVT*YVKL*HTYI*NGNAESCEMSCVLCSLY LCS*FVCL*LKCGSKDS*LLCICNPQRFWAAATS VSSLYYHSLV*INYIVT